RchiOBHm_Chr6g0275071
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0014921
Physical Location & Seq
Forward (+)
36715659 .. 36719805
4147 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24676

Sequence Viewer

Length: 3237 bp
ATGGAAAACCAAAGACTCAGCAGTACTACTTTTTCCTTCACATTGTTCATCATTTTCATAACAATGTCCAAACTAACCTTGGTCTCCTCTCTGTCCCCTGATGGCCAAGCTCTTCTCTCCCTTCTTCCTGCTAAGCAATCTTCCTCTGTACTCTCCTCATGGAACCCATCAAACCAGACACCATGTTCATGGCAAGGAATCACATGCTCACCACAAAACAGAGTCATCTCCCTCTCTCTCCCCAACGTTTTCCTCAATCTCTCCTCTTTACCCCCACAGCTCTCCTCTCTCTCATCTCTCCAGCTTCTCAATCTCTCCTCCACCAACATTTCAGGCACTATCCCACCTTCCTTTGGACAACTTACTCATCTTCGCCTCCTCGACCTCTCCTCCAATTCTCTGTTTGGTCCGATTCCTCCAGAACTAGGCGCTCTCTCTGCTCTCCAGTTCCTCTTCATGAACTCAAACAGATTGTCAGATAAAATCCCTCAACAACTTGCCAACCTCACTTCACTTCAAGTCCTTTGTCTCCAAGACAATCTCATCAACGGCTCGATACCCTCCCAACTCGGTTCTTTGGCTTCCCTCCAACAGTTTCGTGTTGGTGGGAATCCATATATAACCGGAGAAATTCCTTCCCAGTTGGGACTACTCACCAATCTCACCACATTCGGAGCTGCGGCTACTGGACTTTCCGGGACTATTCCACCCACATTTGGAAACTTGATCAATCTTCAGACACTGGCGCTCTATGATACTGAAATAGTTGGTTCAATACCACCCGAAATCGGGCTTTGTTCTGAGCTGAGGAACTTGTACCTGCACATGAACAAGCTCACTGGCTCCATTCCTCCACAATTAGGTAAGCTGCGAAAGCTCACCAGCCTGCTTGTTTGGGGAAATGACTTATCAGGATCAATCCCCGCTGAGATTTCCAACTGTTCCTCACTTGTTGTTCTCGATGCTTCTGCAAATGATCTCACTGGAGAGATTCCAAGAGACTTGGGGAAGCTGGTGCTCCTTGAACAGCTTCACCTATCGGATAACTCGCTTACAGGGTCGATTCCATCGCAGCTCAGCAACTGTAGCAGCCTTACAGCTCTCCAGCTCGACAAGAATCAGTTATCAGGGACAATTCCATGGCAGGTTGGTAATCTGAAATACTTGCAGAGTTTCTTCTTGTGGGGCAATGCAGTGTCTGGAACTATTCCTGCCTCGTTTGGGAACTGCACTGAACTGTATGCACTTGATCTTTCGAGAAACAAGCTCACCGGGTCAATCCCAGAAGAGATTTTCGGTTTGAAGAAACTGAGCAAGCTTTTGCTTCTGGGAAATTCTTTATCCGGCAGGTTGCCACCTAGTGTAGCAAAATGTCAATCTCTAGTGCGGCTGAGACTCGGTGAGAACCAGCTTTCAGGACAAATTCCAAAGGAGATAGGCCAACTGCAAAACCTTGTGTTCCTTGACTTGTACATGAATCATTTCTCTGGAGGCCTTCCCATTGAGATTGCCAACATCACAGTTCTTGAGCTACTGGATGTGCACAATAACTACATTGGTGGCGAAATCCCATCTCAGTTTGGGGAGCTTGTAAATTTGGAGCAGCTTGATCTGAGCCGAAACAGCTTCAAGGGTGAGATTCCTTGGAGCTTTGGGAACTTGAGTTACTTGAACAAGCTCATCATCAACAACAATCTTCTCACAGGTTCAATACCAAAGTCTTTTCGAAATTTGCAGAAGCTGACGTTGTTGGATTTGAGCTTCAACAGCCTCTCTGGTGCAATCCCATCTGAAATCGGTCATGTGACAAGCTTGCAAATCAGTTTGGACTTGAGTTCCAATGGTTTTACAGGAGAACTTCCGGAGACCATGGCGGCTTTGACACAGTTACAGTGTCTGGATCTTTCCCATAACATGCTCTTTGGGAAAATTAAGGTTCTTGGTTCTCTAACTAGTCTCGCTTCCCTTAATATCTCCTGCAATGATTTCTCAGGTGCTATCCCAATAACCACCCCGTTCTTCAGAACTCTCTCCTCAAATTCTTACCTTCAAAATCCACATATTTGTGAGTCTAATGATGGCTCTACTTGCTCTTCAAGTCTAATGAGGAAGAATGGTTTGAAATCTTCGAAAACTATTGCTTTAATTTCTGTGATTCTCTCTTCCGTAACCATAGCAGTTGTTGCTTCATGGATTCTTGTGATGCGAAATCATAGATATATGGTAGAGAAATCTTTAGGAGCATTAGCATCCTCGTCGGGGGCAGAAGATTTCTCCTATCCGTGGACTTTCATCCCATTCCAGAAGTTCAATTTCACTATTGACAACATCTTGGATTGTTTGAAAGAAGAAAATGTGATTGGAAAAGGATGTTCTGGGGTCGTCTACAAGGCAGAAATGCAAACCGGAGAGCTAATTGCGGTGAAAAAGTTGTGGAAGACAAAGCGAGAGGAAGAACCAATAGACTCTTTCGCTGCAGAGATTCAAATTCTTGGACACATTCGCCACAGGAACATTGTGAAGCTCCTAGGGTACTGTTCAAATAGAAGCGTCAAGCTACTTCTTTACAATTTCATTCCCAATGGCAATCTACAACAACTATTGCAAGACAATAGGAACTTGGACTGGGAAACTAGGTACAAGATTGCTATTGGGTCAGCTCAGGGTCTGGCATACCTTCATCATGATTGTGTCCCAGCAATTCTTCACAGGGATGTGAAGTGCAATAACATATTACTAGATTCCAAGTGCGAAGCTTATCTTGCAGATTTTGGTCTTGCGAAATTGATGAACTCCCCAACTTACCATCATGCTATGTCCAGAGTTGCTGGCTCTTATGGTTACATTGCACCAGAGTATGGATACACAATGAACATAACAGAGAAGAGTGATGTCTACAGCTATGGTGTGGTTTTGCTAGAGATCCTAAGTGGACGTAATGCCGTTCAGCCCCAGATTGGTGATGGACTACACATTGTTGAATGGGTGAAGAAGAAGATGGGAAGTTTCGAACCAGCTTTATCAGTACTAGATACAAAGCTCCAAGGCCTGCCAGATCAAATGGTTCAAGAGATGCTGCAAACACTTGGAATAGCAATGTTTTGTGTCAACTCTTCGCCGGCAGAACGACCCACCATGAAGGAAGTGGTGGCATTGCTAATGGAGGTTAAGAGCCAACCTGAAGAATGGGGAAAAACTTCTCAACCCCTAATAAAGCAGTCTTCAAATCAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1078

Amino Acids

117.54

Weight (kDa)

6.41

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 32 - 70 4.1e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 86 - 224 1.3e-12 Leucine-rich repeat region
LRR_8 PF13855 389 - 447 2e-07 Leucine rich repeat
LRR_14 PF23598 408 - 491 7.5e-06 Leucine-rich repeat region
LRR_14 PF23598 503 - 586 1.9e-06 Leucine-rich repeat region
LRR_8 PF13855 556 - 615 5.6e-06 Leucine rich repeat
LRR_14 PF23598 594 - 660 2.9e-07 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 782 - 1052 1.2e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 783 - 1050 1.2e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34110 AT1G34110
fragaria_vesca FvH4_2g13450
malus_domestica MD05G1035300.v1.1 MD10G1037600.v1.1
prunus_persica Prupe.8G043000_v2.0.a1
pyrus_communis pycom05g02560
rosa_chinensis RchiOBHm_Chr6g0275071
rosa_laevigata RLG00000013500
rosa_multiflora Rmu_sc0000447.1_g000012
rosa_roxburghii Rroxscaffold_7G00193690
rosa_rugosa Rorug06G0087900
rosa_samantha Rh6AG202200 Rh6BG205600 Rh6CG207500 Rh6DG197400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 828, 1135, 1338
AccB7I CCANNNNNTGG 1 cut(s) 2858
AccI GTMKAC 2 cut(s) 2385, 2895
AccIII TCCGGA 1 cut(s) 1861
AciI CCGC 5 cut(s) 680, 924, 1387, 1874, 2420
AclI AACGTT 1 cut(s) 246
AclWI GGATC 3 cut(s) 922, 1908, 2917
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 7 cut(s) 630, 1333, 1422, 1594, 1729, 2038, 2487
AcuI CTGAAG 3 cut(s) 719, 2005, 3201
AdeI CACNNNGTG 1 cut(s) 1361
AfaI GTAC 7 cut(s) 25, 150, 818, 1472, 2534, 2639, 3027
AhlI ACTAGT 1 cut(s) 1952
AjuI GAANNNNNNNTTGG 2 cut(s) 2343, 2375
Alw21I GWGCWC 2 cut(s) 1020, 1545
Alw26I GTCTC 6 cut(s) 88, 533, 993, 1387, 1859, 1961
Alw44I GTGCAC 1 cut(s) 1541
AlwI GGATC 3 cut(s) 922, 1908, 2917
AlwNI CAGNNNCTG 6 cut(s) 742, 1083, 1199, 1741, 1897, 2668
Aor13HI TCCGGA 1 cut(s) 1861
AoxI GGCC 4 cut(s) 103, 1438, 1492, 3046
ApaLI GTGCAC 1 cut(s) 1541
ApeKI GCWGC 7 cut(s) 677, 868, 1072, 1089, 1603, 2474, 3076
ApoI RAATTY 7 cut(s) 630, 1333, 1422, 1594, 1729, 2038, 2487
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
Asp700I GAANNNNTTC 3 cut(s) 1029, 1481, 3196
AspA2I CCTAGG 1 cut(s) 2527
AspLEI GCGC 2 cut(s) 431, 748
AspS9I GGNCC 1 cut(s) 407
AsuC2I CCSGG 2 cut(s) 697, 1273
AsuII TTCGAA 3 cut(s) 1726, 2129, 3009
AvaII GGWCC 1 cut(s) 407
AvrII CCTAGG 1 cut(s) 2527
BaeGI GKGCMC 1 cut(s) 1545
BalI TGGCCA 1 cut(s) 105
BbsI GAAGAC 2 cut(s) 2444, 3213
Bbv12I GWGCWC 2 cut(s) 1020, 1545
BbvCI CCTCAGC 1 cut(s) 806
BbvI GCAGC 7 cut(s) 664, 855, 1084, 1101, 1615, 2461, 3063
BccI CCATC 9 cut(s) 95, 175, 1075, 1579, 1795, 2072, 2814, 2957, 2992
BceAI ACGGC 2 cut(s) 565, 2927
BcgI CGANNNNNNTGC 4 cut(s) 950, 984, 2238, 2272
BciVI GTATCC 1 cut(s) 2855
BclI TGATCA 1 cut(s) 726
BcnI CCSGG 2 cut(s) 697, 1273
BcoDI GTCTC 6 cut(s) 88, 533, 993, 1387, 1859, 1961
BcuI ACTAGT 1 cut(s) 1952
BfaI CTAG 9 cut(s) 425, 1359, 1382, 1953, 2528, 2634, 2738, 2918, 3029
BfmI CTRYAG 3 cut(s) 1084, 2475, 2896
BfoI RGCGCY 2 cut(s) 432, 749
BfuAI ACCTGC 3 cut(s) 828, 1135, 1338
BfuI GTATCC 1 cut(s) 2855
BlnI CCTAGG 1 cut(s) 2527
BlpI GCTNAGC 2 cut(s) 132, 1076
BmcAI AGTACT 2 cut(s) 25, 3027
Bme1390I CCNGG 2 cut(s) 697, 1273
Bme18I GGWCC 1 cut(s) 407
BmgT120I GGNCC 1 cut(s) 407
BmiI GGNNCC 2 cut(s) 164, 844
BmrFI CCNGG 2 cut(s) 697, 1273
BmrI ACTGGG 2 cut(s) 634, 2635
BmsI GCATC 4 cut(s) 952, 2193, 2258, 3063
BmuI ACTGGG 2 cut(s) 634, 2635
BpiI GAAGAC 2 cut(s) 2444, 3213
BpmI CTGGAG 6 cut(s) 284, 402, 428, 1005, 1088, 1509
Bpu10I CCTNAGC 2 cut(s) 806, 2661
Bpu1102I GCTNAGC 2 cut(s) 132, 1076
Bpu14I TTCGAA 3 cut(s) 1726, 2129, 3009
BpuEI CTTGAG 3 cut(s) 1547, 1681, 1852
BpuMI CCSGG 2 cut(s) 697, 1273
BsaI GGTCTC 2 cut(s) 88, 1859
BsaJI CCNNGG 7 cut(s) 78, 1139, 1643, 1869, 2282, 2527, 3043
BsaWI WCCGGW 3 cut(s) 623, 1861, 2405
BsaXI ACNNNNNCTCC 2 cut(s) 374, 404
Bse118I RCCGGY 1 cut(s) 3118
Bse1I ACTGG 8 cut(s) 445, 640, 691, 747, 844, 988, 1539, 2630
Bse3DI GCAATG 5 cut(s) 1195, 1987, 2844, 3102, 3152
BseAI TCCGGA 1 cut(s) 1861
BseDI CCNNGG 7 cut(s) 78, 1139, 1643, 1869, 2282, 2527, 3043
BseGI GGATG 5 cut(s) 1543, 2249, 2292, 2375, 2719
BseMI GCAATG 5 cut(s) 1195, 1987, 2844, 3102, 3152
BseNI ACTGG 8 cut(s) 445, 640, 691, 747, 844, 988, 1539, 2630
BseRI GAGGAG 8 cut(s) 76, 145, 253, 274, 307, 368, 379, 2023
BseSI GKGCMC 1 cut(s) 1545
BseXI GCAGC 7 cut(s) 664, 855, 1084, 1101, 1615, 2461, 3063
BseYI CCCAGC 1 cut(s) 2695
BsgI GTGCAG 2 cut(s) 806, 1213
BshFI GGCC 4 cut(s) 105, 1440, 1494, 3048
BsiHKAI GWGCWC 2 cut(s) 1020, 1545
BsiSI CCGG 7 cut(s) 624, 696, 1272, 1344, 1862, 2406, 3119
BslFI GGGAC 5 cut(s) 79, 660, 712, 1144, 2678
BsmAI GTCTC 6 cut(s) 88, 533, 993, 1387, 1859, 1961
BsmFI GGGAC 5 cut(s) 79, 660, 712, 1144, 2678
BsnI GGCC 4 cut(s) 105, 1440, 1494, 3048
Bso31I GGTCTC 2 cut(s) 88, 1859
Bsp119I TTCGAA 3 cut(s) 1726, 2129, 3009
Bsp1286I GDGCHC 2 cut(s) 1020, 1545
Bsp13I TCCGGA 1 cut(s) 1861
Bsp1407I TGTACA 1 cut(s) 1470
Bsp143I GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2922, 3055
Bsp1720I GCTNAGC 2 cut(s) 132, 1076
Bsp19I CCATGG 2 cut(s) 1139, 1869
BspACI CCGC 5 cut(s) 680, 924, 1387, 1874, 2420
BspANI GGCC 4 cut(s) 105, 1440, 1494, 3048
BspEI TCCGGA 1 cut(s) 1861
BspHI TCATGA 2 cut(s) 456, 2683
BspLI GGNNCC 2 cut(s) 164, 844
BspMAI CTGCAG 1 cut(s) 2479
BspMI ACCTGC 3 cut(s) 828, 1135, 1338
BspPI GGATC 3 cut(s) 922, 1908, 2917
BspQI GCTCTTC 2 cut(s) 117, 2098
BspT104I TTCGAA 3 cut(s) 1726, 2129, 3009
BspTNI GGTCTC 2 cut(s) 88, 1859
BsrDI GCAATG 5 cut(s) 1195, 1987, 2844, 3102, 3152
BsrFI RCCGGY 1 cut(s) 3118
BsrGI TGTACA 1 cut(s) 1470
BsrI ACTGG 8 cut(s) 445, 640, 691, 747, 844, 988, 1539, 2630
BssAI RCCGGY 1 cut(s) 3118
BssECI CCNNGG 7 cut(s) 78, 1139, 1643, 1869, 2282, 2527, 3043
BssMI GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2922, 3055
BssT1I CCWWGG 6 cut(s) 78, 1139, 1643, 1869, 2527, 3043
Bst4CI ACNGT 8 cut(s) 594, 941, 1085, 1239, 1522, 1887, 1893, 2537
Bst6I CTCTTC 7 cut(s) 117, 458, 1281, 2098, 2167, 2879, 3118
BstAPI GCANNNNNTGC 1 cut(s) 2183
BstAUI TGTACA 1 cut(s) 1470
BstBI TTCGAA 3 cut(s) 1726, 2129, 3009
BstC8I GCNNGC 6 cut(s) 887, 1316, 1814, 2830, 3050, 3120
BstDSI CCRYGG 3 cut(s) 1139, 1869, 2282
BstF5I GGATG 5 cut(s) 1543, 2249, 2292, 2375, 2719
BstH2I RGCGCY 2 cut(s) 432, 749
BstHHI GCGC 2 cut(s) 431, 748
BstKTI GATC 8 cut(s) 729, 917, 979, 1252, 1612, 1903, 2925, 3058
BstMAI GTCTC 6 cut(s) 88, 533, 993, 1387, 1859, 1961
BstMBI GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2922, 3055
BstMWI GCNNNNNNNGC 8 cut(s) 437, 874, 1086, 1623, 1767, 2088, 2183, 2762
BstNSI RCATGY 2 cut(s) 207, 1918
BstSCI CCNGG 2 cut(s) 695, 1271
BstSFI CTRYAG 3 cut(s) 1084, 2475, 2896
BstSLI GKGCMC 1 cut(s) 1545
BstV1I GCAGC 7 cut(s) 664, 855, 1084, 1101, 1615, 2461, 3063
BstV2I GAAGAC 2 cut(s) 2444, 3213
BstX2I RGATCY 2 cut(s) 1900, 2922
BstXI CCANNNNNNTGG 1 cut(s) 189
BstYI RGATCY 2 cut(s) 1900, 2922
BsuI GTATCC 1 cut(s) 2855
BsuRI GGCC 4 cut(s) 105, 1440, 1494, 3048
BtgI CCRYGG 3 cut(s) 1139, 1869, 2282
BtgZI GCGATG 1 cut(s) 1053
BtsCI GGATG 5 cut(s) 1543, 2249, 2292, 2375, 2719
BtsI GCAGTG 1 cut(s) 1200
BtsIMutI CAGTG 6 cut(s) 740, 837, 981, 1200, 1230, 1898
BveI ACCTGC 3 cut(s) 828, 1135, 1338
Cac8I GCNNGC 6 cut(s) 887, 1316, 1814, 2830, 3050, 3120
CaiI CAGNNNCTG 6 cut(s) 742, 1083, 1199, 1741, 1897, 2668
CciI TCATGA 2 cut(s) 456, 2683
CfoI GCGC 2 cut(s) 431, 748
Cfr10I RCCGGY 1 cut(s) 3118
Cfr13I GGNCC 1 cut(s) 407
CseI GACGC 1 cut(s) 2539
Csp6I GTAC 7 cut(s) 24, 149, 817, 1471, 2533, 2638, 3026
CviQI GTAC 7 cut(s) 24, 149, 817, 1471, 2533, 2638, 3026
DpnI GATC 8 cut(s) 728, 916, 978, 1251, 1611, 1902, 2924, 3057
DpnII GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2922, 3055
DraIII CACNNNGTG 1 cut(s) 1361
EaeI YGGCCR 1 cut(s) 103
Eam1104I CTCTTC 7 cut(s) 117, 458, 1281, 2098, 2167, 2879, 3118
EarI CTCTTC 7 cut(s) 117, 458, 1281, 2098, 2167, 2879, 3118
Eco130I CCWWGG 6 cut(s) 78, 1139, 1643, 1869, 2527, 3043
Eco147I AGGCCT 2 cut(s) 1494, 3048
Eco31I GGTCTC 2 cut(s) 88, 1859
Eco47I GGWCC 1 cut(s) 407
Eco57I CTGAAG 3 cut(s) 719, 2005, 3201
EcoT14I CCWWGG 6 cut(s) 78, 1139, 1643, 1869, 2527, 3043
ErhI CCWWGG 6 cut(s) 78, 1139, 1643, 1869, 2527, 3043
FalI AAGNNNNNCTT 2 cut(s) 2745, 2777
FaqI GGGAC 5 cut(s) 79, 660, 712, 1144, 2678
FauI CCCGC 1 cut(s) 931
FbaI TGATCA 1 cut(s) 726
FblI GTMKAC 2 cut(s) 2385, 2895
FokI GGATG 5 cut(s) 1550, 2236, 2279, 2382, 2726
FspBI CTAG 9 cut(s) 425, 1359, 1382, 1953, 2528, 2634, 2738, 2918, 3029
GlaI GCGC 2 cut(s) 430, 747
GsaI CCCAGC 1 cut(s) 2699
GsuI CTGGAG 6 cut(s) 284, 402, 428, 1005, 1088, 1509
HaeII RGCGCY 2 cut(s) 432, 749
HaeIII GGCC 4 cut(s) 105, 1440, 1494, 3048
HapII CCGG 7 cut(s) 624, 696, 1272, 1344, 1862, 2406, 3119
HgaI GACGC 1 cut(s) 2539
HhaI GCGC 2 cut(s) 431, 748
Hin6I GCGC 2 cut(s) 429, 746
HinP1I GCGC 2 cut(s) 429, 746
HincII GTYRAC 1 cut(s) 3109
HindII GTYRAC 1 cut(s) 3109
HindIII AAGCTT 3 cut(s) 1316, 1810, 2754
HpaII CCGG 7 cut(s) 624, 696, 1272, 1344, 1862, 2406, 3119
Hpy166II GTNNAC 6 cut(s) 1543, 2286, 2386, 2896, 2933, 3109
Hpy8I GTNNAC 6 cut(s) 1543, 2286, 2386, 2896, 2933, 3109
Hpy99I CGWCG 1 cut(s) 2260
HpyAV CCTTC 8 cut(s) 46, 131, 357, 645, 1505, 2057, 2687, 3133
HpyCH4III ACNGT 8 cut(s) 594, 941, 1085, 1239, 1522, 1887, 1893, 2537
HpyCH4IV ACGT 3 cut(s) 246, 1745, 2935
HpyF10VI GCNNNNNNNGC 8 cut(s) 437, 874, 1086, 1623, 1767, 2088, 2183, 2762
HpySE526I ACGT 3 cut(s) 246, 1745, 2935
HspAI GCGC 2 cut(s) 429, 746
Kpn2I TCCGGA 1 cut(s) 1861
KroI GCCGGC 1 cut(s) 3118
KroNI GCCGGC 1 cut(s) 3120
Ksp22I TGATCA 1 cut(s) 726
Kzo9I GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2922, 3055
LguI GCTCTTC 2 cut(s) 117, 2098
LmnI GCTCC 9 cut(s) 674, 848, 1023, 1585, 1600, 1647, 2240, 2529, 3045
Lsp1109I GCAGC 7 cut(s) 664, 855, 1084, 1101, 1615, 2461, 3063
LweI GCATC 4 cut(s) 952, 2193, 2258, 3063
MaeI CTAG 9 cut(s) 425, 1359, 1382, 1953, 2528, 2634, 2738, 2918, 3029
MaeII ACGT 3 cut(s) 246, 1745, 2935
MaeIII GTNAC 5 cut(s) 1664, 1804, 1887, 2167, 2840
MalI GATC 8 cut(s) 728, 916, 978, 1251, 1611, 1902, 2924, 3057
MboI GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2922, 3055
MflI RGATCY 2 cut(s) 1900, 2922
MhlI GDGCHC 2 cut(s) 1020, 1545
MlsI TGGCCA 1 cut(s) 105
MluNI TGGCCA 1 cut(s) 105
MlyI GAGTC 5 cut(s) 9, 231, 1389, 2078, 2459
MmeI TCCRAC 3 cut(s) 613, 960, 1731
Mox20I TGGCCA 1 cut(s) 105
MroI TCCGGA 1 cut(s) 1861
MroNI GCCGGC 1 cut(s) 3118
MroXI GAANNNNTTC 3 cut(s) 1029, 1481, 3196
MscI TGGCCA 1 cut(s) 105
MseI TTAA 5 cut(s) 1932, 1968, 2144, 3168, 3235
MslI CAYNNNNRTG 5 cut(s) 62, 187, 2064, 2688, 2712
Msp20I TGGCCA 1 cut(s) 105
MspA1I CMGCKG 1 cut(s) 926
MspI CCGG 7 cut(s) 624, 696, 1272, 1344, 1862, 2406, 3119
MspR9I CCNGG 2 cut(s) 697, 1273
MwoI GCNNNNNNNGC 8 cut(s) 437, 874, 1086, 1623, 1767, 2088, 2183, 2762
NaeI GCCGGC 1 cut(s) 3120
NciI CCSGG 2 cut(s) 697, 1273
NcoI CCATGG 2 cut(s) 1139, 1869
NdeII GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2922, 3055
NgoMIV GCCGGC 1 cut(s) 3118
NlaIV GGNNCC 2 cut(s) 164, 844
NmuCI GTSAC 1 cut(s) 1804
NspI RCATGY 2 cut(s) 207, 1918
NspV TTCGAA 3 cut(s) 1726, 2129, 3009
PagI TCATGA 2 cut(s) 456, 2683
PceI AGGCCT 2 cut(s) 1494, 3048
PciSI GCTCTTC 2 cut(s) 117, 2098
PdiI GCCGGC 1 cut(s) 3120
PdmI GAANNNNTTC 3 cut(s) 1029, 1481, 3196
PflMI CCANNNNNTGG 1 cut(s) 2858
PfoI TCCNGGA 1 cut(s) 695
PleI GAGTC 5 cut(s) 9, 230, 1389, 2077, 2459
PpsI GAGTC 5 cut(s) 9, 230, 1389, 2077, 2459
Psp1406I AACGTT 1 cut(s) 246
PspFI CCCAGC 1 cut(s) 2695
PspN4I GGNNCC 2 cut(s) 164, 844
PspPI GGNCC 1 cut(s) 407
PsrI GAACNNNNNNTAC 2 cut(s) 1649, 1681
PstI CTGCAG 1 cut(s) 2479
PstNI CAGNNNCTG 6 cut(s) 742, 1083, 1199, 1741, 1897, 2668
PsuI RGATCY 2 cut(s) 1900, 2922
RsaI GTAC 7 cut(s) 25, 150, 818, 1472, 2534, 2639, 3027
RsaNI GTAC 7 cut(s) 24, 149, 817, 1471, 2533, 2638, 3026
RseI CAYNNNNRTG 5 cut(s) 62, 187, 2064, 2688, 2712
SapI GCTCTTC 2 cut(s) 117, 2098
SaqAI TTAA 5 cut(s) 1932, 1968, 2144, 3168, 3235
Sau3AI GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2922, 3055
Sau96I GGNCC 1 cut(s) 407
ScaI AGTACT 2 cut(s) 25, 3027
SchI GAGTC 5 cut(s) 9, 231, 1389, 2078, 2459
ScrFI CCNGG 2 cut(s) 697, 1273
SduI GDGCHC 2 cut(s) 1020, 1545
SfaNI GCATC 4 cut(s) 952, 2193, 2258, 3063
SfcI CTRYAG 3 cut(s) 1084, 2475, 2896
SfuI TTCGAA 3 cut(s) 1726, 2129, 3009
SinI GGWCC 1 cut(s) 407
SmiMI CAYNNNNRTG 5 cut(s) 62, 187, 2064, 2688, 2712
SmlI CTYRAG 3 cut(s) 1526, 1660, 1831
SmoI CTYRAG 3 cut(s) 1526, 1660, 1831
SpeI ACTAGT 1 cut(s) 1952
SseBI AGGCCT 2 cut(s) 1494, 3048
SsiI CCGC 5 cut(s) 680, 924, 1387, 1874, 2420
SspMI CTAG 9 cut(s) 425, 1359, 1382, 1953, 2528, 2634, 2738, 2918, 3029
StuI AGGCCT 2 cut(s) 1494, 3048
StyD4I CCNGG 2 cut(s) 695, 1271
StyI CCWWGG 6 cut(s) 78, 1139, 1643, 1869, 2527, 3043
TaaI ACNGT 8 cut(s) 594, 941, 1085, 1239, 1522, 1887, 1893, 2537
TaiI ACGT 3 cut(s) 249, 1748, 2938
TaqI TCGA 9 cut(s) 381, 554, 960, 1061, 1110, 1256, 1726, 2129, 3009
TaqII GACCGA 1 cut(s) 1787
TatI WGTACW 4 cut(s) 23, 148, 1470, 3025
TauI GCSGC 3 cut(s) 683, 1390, 1877
Tru1I TTAA 5 cut(s) 1932, 1968, 2144, 3168, 3235
Tru9I TTAA 5 cut(s) 1932, 1968, 2144, 3168, 3235
TscAI CASTG 6 cut(s) 747, 844, 988, 1200, 1237, 1898
TseFI GTSAC 1 cut(s) 1804
TseI GCWGC 7 cut(s) 677, 868, 1072, 1089, 1603, 2474, 3076
Tsp45I GTSAC 1 cut(s) 1804
TspGWI ACGGA 2 cut(s) 2155, 2271
TspRI CASTG 6 cut(s) 747, 844, 988, 1200, 1237, 1898
Van91I CCANNNNNTGG 1 cut(s) 2858
VneI GTGCAC 1 cut(s) 1541
VpaK11BI GGWCC 1 cut(s) 407
XapI RAATTY 7 cut(s) 630, 1333, 1422, 1594, 1729, 2038, 2487
XceI RCATGY 2 cut(s) 207, 1918
XcmI CCANNNNNNNNNTGG 3 cut(s) 76, 2960, 3142
XmaJI CCTAGG 1 cut(s) 2527
XmiI GTMKAC 2 cut(s) 2385, 2895
XmnI GAANNNNTTC 3 cut(s) 1029, 1481, 3196
XspI CTAG 9 cut(s) 425, 1359, 1382, 1953, 2528, 2634, 2738, 2918, 3029
ZrmI AGTACT 2 cut(s) 25, 3027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.