MD05G1035300.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014921
Physical Location & Seq
Forward (+)
5703360 .. 5706692
3333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1035300.v1.1.491

Sequence Viewer

Length: 3225 bp
ATGGTCCAAAACACAACCCATTTACTATTTTCCTTCAGATTGTTGATTTTCATAACCATGACCAAAATCTCATTAATTACCTCTCTGTCCTCTGATGGCCAAGCCCTCCTCTCCCTCCTCCCTGAAAAGCAATATTCTGTGCTTTCCTCATGGGACTCATCAAGTGAAACACCATGCTCATGGCAAGGGATAACATGCTCTCCCCAAAACAGAGTAATCTCACTCTCTCTCCCCAACATTTTCCTCAACCTCTCCTCCCTCCCTCCGCATCTCTCCTCCCTCTCATATCTCCAGCTCCTCAACCTCTCCTCCACCAACATATCCGGCACCATCCCACCTTCCTTTGGCCAACTCACCCACCTCCGCCTCCTCGACTTGTCCTCCAACTCCCTCACCGGCCCCATTCCACCGGAGCTAGGCAACCTCTCCGCTCTCCAGTTCCTTTTACTCAACTCCAACAAACTTTCAGAGAAAATACCTCAACAACTTGCCAACTTCATTTCCCTCCAAGTGCTTTGTCTCCAAGACAACCTCATCAACGGCCCAATCCCATCTCAGTTGGGCTCTCTGGTTTCCCTCCAACAGTTTCGTGTTGGAGGGAATCCGTATATCACCGGCGAAATCCCATCCCAACTAGGCCTGCTCACTAACCTCACAACTTTTGGCGCAGCGGCTACCTCCCTTTCGGGGGTGATCCCATCCACATTTGGGAACTTGATCAACCTCCAAACACTGGCTCTTTATGATACTGAGATATTTGGCTCAATACCTCCGGAACTCGGACTTTGTTCGGAGCTGAGGGATTTGTATTTGCATATGAACAAGCTCACTGGTTCAATTCCTCCACAGCTGGGGAAACTGCAAAAGCTCACCAACCTGCTTGTTTGGGGCAATGCATTGTCAGGACCAATCCCAGCTGAGATTTCAAACTGTTCTTCCCTTGTTATTCTCGATGCTTCTGCCAATGATCTCCCTGGACCAATCCCAACAGACTTGGGGAAGCTGGTGGTTCTCGAACAGCTTCATCTCTCCGATAATATGCTGACCGGAGCAATTCCATCCCAGCTCAGCAACTGCACCAGCCTCACTGCTCTTCAGCTTGACAAGAATCAGTTGTCAGGTACAATTCCTTGGCAGGTTGGCAATCTCAAACTCTTGCAGAGTTTTTTCTTGTGGGGCAATATTGTATCGGGAACTATACCAGCTTCGTTCGGCAACTGCACTGAGCTCTATGCACTTGATCTCTCAAGAAACAAGCTTACTGGGTCGATCCCAGAAGAGATTTTCGGTTTGAAGAAGCTGAGCAAGCTCTTGCTGTTGGGGAATTCTTTGTCGGGCGGGTTGCCACGAAGCGTTGCACATTGTCAATCTCTAGTCAGGTTAAGGCTTGGGGAGAACCAGCTTTCAGGACAGATTCCTATGGAGATAGGCAAGCTGCAAAACCTCGTTTTCCTTGACTTGTACATGAATCATTTCTCTGGAGGATTGCCGTTTGAGATTGCCAACATCACAGTTCTTGAAATGTTGGATGTGCACAACAACAATATCAGTGGTGAAATCCCGCCTCAGCTTGGGGAGCTTGTGAATTTGGAGCAGCTTGATCTTAGCAGAAACAGCTTTACCGGAGAAATTCCTTGGAGCTTTGGCAACTTAAGTTACTTGGACAAGCTCATTGCCAACAACAATCTACTCACGGGTACGATACCAAAATCTATCCGGAACCTGCAGAAGCTAACTCTGCTGGATTTGAGCTTTAACAGCCTCTCCGGTCCAATCCCACCCGAAATTGGTCGTGTAACAAGCTTGACAATCAGTTTGGACTTGAGCTCCAATAGTTTTACTGGAGAAATCCCAGAGACAATGGCGGATTTGATGCAGTTGCAATCACTGGACCTGTCGCATAATATGCTCTATGGAAAAATTAAGGTTCTTGGTTCTCTAACTAGTCTCACTTCCCTGAATATTTCCTGCAACAATTTTTCGGGTGCCATCCCAGTTACGCCATTCTTTAGAACTCTTTCTTCGAATTCATATGCTCAGAATCCACATCTTTGTGAGTCTGTTGATGGGACTACTTGTTCGTCAAGTCTAATGCTGAAGAATGGTTTGAAATCCGCAAAAACTGTTGCTTTAATTACTGTGATTCTGGTGTCAGTAACCATGGCAGTTGTTTCCTCGTGGATTCTTGTTATGCGAAATCATAGACATATGGTAAAGAAATCTTTAGGAGCAATGGCAGCCTCGTCGGGGGCTGAAGATTTCTCGTATCCATGGACTTTCATCCCATTTCAGAAGCTCAATTTCACCATTGATAATATCTTGGATTGTTTGAAGGAGGAAAATGTGATTGGAAAAGGTTGTTCTGGAATCGTCTACAAGGCTGAATTGCATAATGGGGATTTGATTGCGGTGAAAAAACTGTGGAAAACGAAGCAAGAGGAAGAACCGATAGACTCTTTTGCTGCAGAGATTCAAATTCTTGGACACATTCGCCATCGGAACATTGTCAAACTCCTAGGTTACTGTTCGAATAGAAGCGTCAAGCTGCTTCTTTATAACTACATTCCCAATGGTAATCTGCAGCAGCTATTGCAAGGGAATAGGAACTTGGATTGGGAAACTCGGTACAAGATTGCAATCGGATCAGCTCAAGGTTTAGCATACCTTCATCACGATTGTGTCCCGACAATTCTGCACAGGGATGTCAAGTGCAATAACATACTGCTGGATTCAAAGTATGAGGCCTATCTGGCAGATTTTGGTCTGGCAAAGCTGATGAACTCCCCAACTTACCACCACGCAATGTCGAGAGTAGCTGGCTCTTATGGTTATATTGCCCCAGAGTACGGATACACGATGAACATAACAGAGAAGAGTGATGTCTACAGCTATGGTGTGGTTCTGCTGGAGATCTTAAGCGGGCGTAGTGCTGTACAGCCACAGATTGGTGACGGGCTACACATTGTTGAATGGGTGAAGAAGAAAATGGGAAGCTTCGAACCAGCTGTATCGATACTAGATACAAAGCTCCAAGGCCTACCGGATCAAATGGTGCAAGAGATGCTGCAAACACTTGGAATAGCAATGTTCTGTGTAAACTCGTCGCCTGCAGAAAGACCAAGCATGAAGGAAGTGGTGGCATTGCTAATGGAGGTTAAGAGCCAGCCTGAAGAATGCGGAAAGACCTCCCAACCCCTAATAAAGCAATCTTCAAACCAGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1075

Amino Acids

117.19

Weight (kDa)

6.18

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 30 - 67 7e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 83 - 174 1e-08 Leucine-rich repeat region
LRR_14 PF23598 85 - 221 7.7e-12 Leucine-rich repeat region
LRR_8 PF13855 96 - 155 4.9e-07 Leucine rich repeat
LRR_14 PF23598 162 - 298 1.3e-08 Leucine-rich repeat region
LRR_8 PF13855 386 - 444 7.6e-07 Leucine rich repeat
LRR_14 PF23598 453 - 557 3.9e-08 Leucine-rich repeat region
LRR_14 PF23598 475 - 581 2.6e-08 Leucine-rich repeat region
LRR_14 PF23598 567 - 660 1e-06 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 778 - 1048 2.5e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 779 - 1046 4.1e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34110 AT1G34110
fragaria_vesca FvH4_2g13450
malus_domestica MD05G1035300.v1.1 MD10G1037600.v1.1
prunus_persica Prupe.8G043000_v2.0.a1
pyrus_communis pycom05g02560
rosa_chinensis RchiOBHm_Chr6g0275071
rosa_laevigata RLG00000013500
rosa_multiflora Rmu_sc0000447.1_g000012
rosa_roxburghii Rroxscaffold_7G00193690
rosa_rugosa Rorug06G0087900
rosa_samantha Rh6AG202200 Rh6BG205600 Rh6CG207500 Rh6DG197400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2556
Acc36I ACCTGC 3 cut(s) 885, 1126, 1731
AccB1I GGYRCC 2 cut(s) 326, 1985
AccB7I CCANNNNNTGG 2 cut(s) 733, 2945
AccBSI CCGCTC 1 cut(s) 431
AccI GTMKAC 2 cut(s) 2373, 2883
AccIII TCCGGA 2 cut(s) 772, 1716
AclWI GGATC 4 cut(s) 688, 1264, 2650, 3051
AcoI YGGCCR 2 cut(s) 97, 346
AcsI RAATTY 5 cut(s) 1324, 1585, 1629, 2026, 2475
AcuI CTGAAG 5 cut(s) 19, 1079, 2117, 2274, 3189
AfaI GTAC 6 cut(s) 1123, 1463, 1699, 2627, 2846, 2934
AflII CTTAAG 2 cut(s) 1651, 2914
AhlI ACTAGT 1 cut(s) 1943
AjnI CCWGG 1 cut(s) 973
AjuI GAANNNNNNNTTGG 4 cut(s) 2331, 2363, 2591, 2623
AleI CACNNNNGTG 1 cut(s) 2676
AloI GAACNNNNNNTCC 2 cut(s) 2062, 2094
Alw21I GWGCWC 3 cut(s) 1230, 1536, 1829
Alw26I GTCTC 3 cut(s) 524, 1850, 1952
Alw44I GTGCAC 1 cut(s) 1532
AlwI GGATC 4 cut(s) 688, 1264, 2650, 3051
AlwNI CAGNNNCTG 1 cut(s) 1074
Aor13HI TCCGGA 2 cut(s) 772, 1716
AoxI GGCC 7 cut(s) 97, 346, 397, 541, 637, 2742, 3034
ApaLI GTGCAC 1 cut(s) 1532
ApeKI GCWGC 9 cut(s) 668, 1435, 1594, 2237, 2462, 2545, 2581, 2584, 3064
ApoI RAATTY 5 cut(s) 1324, 1585, 1629, 2026, 2475
ArsI GACNNNNNNTTYG 4 cut(s) 1798, 1830, 2062, 2094
AseI ATTAAT 1 cut(s) 74
Asp700I GAANNNNTTC 3 cut(s) 1020, 1472, 2017
AspA2I CCTAGG 1 cut(s) 2515
AspLEI GCGC 1 cut(s) 668
AspS9I GGNCC 7 cut(s) 4, 398, 542, 905, 977, 1769, 1891
AsuII TTCGAA 3 cut(s) 2024, 2528, 2997
AvaII GGWCC 5 cut(s) 4, 905, 977, 1769, 1891
AvrII CCTAGG 1 cut(s) 2515
BaeGI GKGCMC 1 cut(s) 1536
BalI TGGCCA 2 cut(s) 99, 348
BanI GGYRCC 2 cut(s) 326, 1985
BanII GRGCYC 3 cut(s) 566, 1230, 1829
BauI CACGAG 1 cut(s) 2176
Bbv12I GWGCWC 3 cut(s) 1230, 1536, 1829
BbvCI CCTCAGC 2 cut(s) 797, 1566
BbvI GCAGC 9 cut(s) 680, 1422, 1606, 2249, 2449, 2532, 2593, 2596, 3051
BccI CCATC 9 cut(s) 89, 338, 559, 634, 706, 1066, 1997, 2060, 2502
BceAI ACGGC 2 cut(s) 556, 1474
BcgI CGANNNNNNTGC 6 cut(s) 941, 975, 2226, 2260, 2674, 2708
BciT130I CCWGG 1 cut(s) 975
BciVI GTATCC 2 cut(s) 2277, 2843
BclI TGATCA 1 cut(s) 717
BcoDI GTCTC 3 cut(s) 524, 1850, 1952
BcuI ACTAGT 1 cut(s) 1943
BfaI CTAG 6 cut(s) 416, 635, 1373, 1944, 2516, 3017
BfmI CTRYAG 5 cut(s) 1724, 2463, 2579, 2884, 3108
BfrI CTTAAG 2 cut(s) 1651, 2914
BfuAI ACCTGC 3 cut(s) 885, 1126, 1731
BfuI GTATCC 2 cut(s) 2277, 2843
BglI GCCNNNNNGGC 1 cut(s) 2750
BglII AGATCT 1 cut(s) 2910
BlnI CCTAGG 1 cut(s) 2515
BlpI GCTNAGC 2 cut(s) 1067, 1301
Bme1390I CCNGG 1 cut(s) 975
Bme18I GGWCC 5 cut(s) 4, 905, 977, 1769, 1891
BmgT120I GGNCC 7 cut(s) 4, 398, 542, 905, 977, 1769, 1891
BmiI GGNNCC 4 cut(s) 328, 400, 1721, 1987
BmrFI CCNGG 1 cut(s) 975
BmrI ACTGGG 2 cut(s) 1272, 1988
BmsI GCATC 4 cut(s) 277, 943, 1863, 3051
BmuI ACTGGG 2 cut(s) 1272, 1988
BpmI CTGGAG 5 cut(s) 275, 419, 1500, 1863, 2927
Bpu10I CCTNAGC 2 cut(s) 797, 1566
Bpu1102I GCTNAGC 2 cut(s) 1067, 1301
Bpu14I TTCGAA 3 cut(s) 2024, 2528, 2997
BpuEI CTTGAG 3 cut(s) 1231, 1843, 2634
Bsa29I ATCGAT 1 cut(s) 3011
BsaBI GATNNNNATC 1 cut(s) 2636
BsaJI CCNNGG 7 cut(s) 973, 1130, 1634, 2160, 2270, 2515, 3031
BsaWI WCCGGW 7 cut(s) 409, 772, 1046, 1622, 1716, 1766, 3040
Bse118I RCCGGY 2 cut(s) 395, 614
Bse1I ACTGG 7 cut(s) 436, 738, 835, 1267, 1846, 1893, 1994
Bse3DI GCAATG 6 cut(s) 898, 1671, 2238, 2808, 3090, 3140
Bse8I GATNNNNATC 1 cut(s) 2636
BseAI TCCGGA 2 cut(s) 772, 1716
BseBI CCWGG 1 cut(s) 975
BseCI ATCGAT 1 cut(s) 3011
BseDI CCNNGG 7 cut(s) 973, 1130, 1634, 2160, 2270, 2515, 3031
BseGI GGATG 8 cut(s) 330, 626, 698, 1058, 1534, 1989, 2280, 2707
BseJI GATNNNNATC 1 cut(s) 2636
BseMI GCAATG 6 cut(s) 898, 1671, 2238, 2808, 3090, 3140
BseMII CTCAG 9 cut(s) 569, 741, 788, 909, 1081, 1215, 1292, 1580, 2051
BseNI ACTGG 7 cut(s) 436, 738, 835, 1267, 1846, 1893, 1994
BseRI GAGGAG 7 cut(s) 98, 107, 244, 265, 287, 298, 359
BseSI GKGCMC 1 cut(s) 1536
BseXI GCAGC 9 cut(s) 680, 1422, 1606, 2249, 2449, 2532, 2593, 2596, 3051
BseYI CCCAGC 3 cut(s) 850, 913, 1062
BsgI GTGCAG 3 cut(s) 1060, 1204, 2678
BshFI GGCC 7 cut(s) 99, 348, 399, 543, 639, 2744, 3036
BshNI GGYRCC 2 cut(s) 326, 1985
BshVI ATCGAT 1 cut(s) 3011
BsiHKAI GWGCWC 3 cut(s) 1230, 1536, 1829
BslFI GGGAC 3 cut(s) 167, 2083, 2666
BsmAI GTCTC 3 cut(s) 524, 1850, 1952
BsmFI GGGAC 3 cut(s) 167, 2083, 2666
BsmI GAATGC 1 cut(s) 3179
BsnI GGCC 7 cut(s) 99, 348, 399, 543, 639, 2744, 3036
Bsp119I TTCGAA 3 cut(s) 2024, 2528, 2997
Bsp1286I GDGCHC 4 cut(s) 566, 1230, 1536, 1829
Bsp13I TCCGGA 2 cut(s) 772, 1716
Bsp1407I TGTACA 2 cut(s) 1461, 2932
Bsp143I GATC 9 cut(s) 693, 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
Bsp1720I GCTNAGC 2 cut(s) 1067, 1301
Bsp19I CCATGG 2 cut(s) 2160, 2270
BspANI GGCC 7 cut(s) 99, 348, 399, 543, 639, 2744, 3036
BspCNI CTCAG 9 cut(s) 568, 742, 789, 910, 1080, 1216, 1293, 1579, 2050
BspDI ATCGAT 1 cut(s) 3011
BspEI TCCGGA 2 cut(s) 772, 1716
BspLI GGNNCC 4 cut(s) 328, 400, 1721, 1987
BspMAI CTGCAG 4 cut(s) 1728, 2467, 2583, 3112
BspMI ACCTGC 3 cut(s) 885, 1126, 1731
BspPI GGATC 4 cut(s) 688, 1264, 2650, 3051
BspQI GCTCTTC 1 cut(s) 1098
BspT104I TTCGAA 3 cut(s) 2024, 2528, 2997
BspT107I GGYRCC 2 cut(s) 326, 1985
BspTI CTTAAG 2 cut(s) 1651, 2914
BsrBI CCGCTC 1 cut(s) 431
BsrDI GCAATG 6 cut(s) 898, 1671, 2238, 2808, 3090, 3140
BsrFI RCCGGY 2 cut(s) 395, 614
BsrGI TGTACA 2 cut(s) 1461, 2932
BsrI ACTGG 7 cut(s) 436, 738, 835, 1267, 1846, 1893, 1994
BssAI RCCGGY 2 cut(s) 395, 614
BssECI CCNNGG 7 cut(s) 973, 1130, 1634, 2160, 2270, 2515, 3031
BssMI GATC 9 cut(s) 693, 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
BssSI CACGAG 1 cut(s) 2176
BssT1I CCWWGG 6 cut(s) 1130, 1634, 2160, 2270, 2515, 3031
Bst2BI CACGAG 1 cut(s) 2176
Bst2UI CCWGG 1 cut(s) 975
Bst4CI ACNGT 7 cut(s) 585, 932, 1513, 2125, 2140, 2421, 2525
Bst6I CTCTTC 3 cut(s) 1098, 1272, 2867
BstAFI CTTAAG 2 cut(s) 1651, 2914
BstAPI GCANNNNNTGC 3 cut(s) 1906, 2590, 3061
BstAUI TGTACA 2 cut(s) 1461, 2932
BstBI TTCGAA 3 cut(s) 2024, 2528, 2997
BstC8I GCNNGC 7 cut(s) 641, 1307, 1433, 2818, 2921, 3108, 3164
BstDSI CCRYGG 2 cut(s) 2160, 2270
BstF5I GGATG 8 cut(s) 330, 626, 698, 1058, 1534, 1989, 2280, 2707
BstHHI GCGC 1 cut(s) 668
BstKTI GATC 9 cut(s) 696, 720, 970, 1243, 1272, 1603, 2645, 2913, 3046
BstMAI GTCTC 3 cut(s) 524, 1850, 1952
BstMBI GATC 9 cut(s) 693, 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
BstNI CCWGG 1 cut(s) 975
BstNSI RCATGY 1 cut(s) 198
BstSCI CCNGG 1 cut(s) 973
BstSFI CTRYAG 5 cut(s) 1724, 2463, 2579, 2884, 3108
BstSLI GKGCMC 1 cut(s) 1536
BstV1I GCAGC 9 cut(s) 680, 1422, 1606, 2249, 2449, 2532, 2593, 2596, 3051
BstX2I RGATCY 1 cut(s) 2910
BstXI CCANNNNNNTGG 1 cut(s) 180
BstYI RGATCY 1 cut(s) 2910
Bsu15I ATCGAT 1 cut(s) 3011
BsuI GTATCC 2 cut(s) 2277, 2843
BsuRI GGCC 7 cut(s) 99, 348, 399, 543, 639, 2744, 3036
BsuTUI ATCGAT 1 cut(s) 3011
BtgI CCRYGG 2 cut(s) 2160, 2270
BtsCI GGATG 8 cut(s) 330, 626, 698, 1058, 1534, 1989, 2280, 2707
BtsI GCAGTG 1 cut(s) 1086
BtsIMutI CAGTG 6 cut(s) 731, 828, 1086, 1221, 1555, 1886
BveI ACCTGC 3 cut(s) 885, 1126, 1731
Cac8I GCNNGC 7 cut(s) 641, 1307, 1433, 2818, 2921, 3108, 3164
CaiI CAGNNNCTG 1 cut(s) 1074
CfoI GCGC 1 cut(s) 668
Cfr10I RCCGGY 2 cut(s) 395, 614
Cfr13I GGNCC 7 cut(s) 4, 398, 542, 905, 977, 1769, 1891
ClaI ATCGAT 1 cut(s) 3011
CseI GACGC 1 cut(s) 2527
Csp6I GTAC 6 cut(s) 1122, 1462, 1698, 2626, 2845, 2933
CspCI CAANNNNNGTGG 2 cut(s) 1531, 1566
CviAII CATG 9 cut(s) 58, 150, 174, 180, 195, 1465, 2161, 2271, 3124
CviQI GTAC 6 cut(s) 1122, 1462, 1698, 2626, 2845, 2933
DpnI GATC 9 cut(s) 695, 719, 969, 1242, 1271, 1602, 2644, 2912, 3045
DpnII GATC 9 cut(s) 693, 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
EaeI YGGCCR 2 cut(s) 97, 346
Eam1104I CTCTTC 3 cut(s) 1098, 1272, 2867
EarI CTCTTC 3 cut(s) 1098, 1272, 2867
EciI GGCGGA 2 cut(s) 353, 1880
Ecl136II GAGCTC 2 cut(s) 1228, 1827
Eco130I CCWWGG 6 cut(s) 1130, 1634, 2160, 2270, 2515, 3031
Eco147I AGGCCT 3 cut(s) 639, 2744, 3036
Eco24I GRGCYC 3 cut(s) 566, 1230, 1829
Eco47I GGWCC 5 cut(s) 4, 905, 977, 1769, 1891
Eco53kI GAGCTC 2 cut(s) 1228, 1827
Eco57I CTGAAG 5 cut(s) 19, 1079, 2117, 2274, 3189
EcoICRI GAGCTC 2 cut(s) 1228, 1827
EcoRI GAATTC 2 cut(s) 1324, 2026
EcoRII CCWGG 1 cut(s) 973
EcoT14I CCWWGG 6 cut(s) 1130, 1634, 2160, 2270, 2515, 3031
EcoT22I ATGCAT 1 cut(s) 898
EcoT38I GRGCYC 3 cut(s) 566, 1230, 1829
ErhI CCWWGG 6 cut(s) 1130, 1634, 2160, 2270, 2515, 3031
FaeI CATG 9 cut(s) 61, 153, 177, 183, 198, 1468, 2164, 2274, 3127
FaqI GGGAC 3 cut(s) 167, 2083, 2666
FatI CATG 9 cut(s) 57, 149, 173, 179, 194, 1464, 2160, 2270, 3123
FauI CCCGC 3 cut(s) 1331, 1569, 2912
FauNDI CATATG 3 cut(s) 816, 2032, 2208
FbaI TGATCA 1 cut(s) 717
FblI GTMKAC 2 cut(s) 2373, 2883
FokI GGATG 8 cut(s) 317, 613, 685, 1045, 1541, 1976, 2267, 2714
FriOI GRGCYC 3 cut(s) 566, 1230, 1829
FspBI CTAG 6 cut(s) 416, 635, 1373, 1944, 2516, 3017
GlaI GCGC 1 cut(s) 667
GsaI CCCAGC 3 cut(s) 854, 917, 1066
GsuI CTGGAG 5 cut(s) 275, 419, 1500, 1863, 2927
HaeIII GGCC 7 cut(s) 99, 348, 399, 543, 639, 2744, 3036
HgaI GACGC 1 cut(s) 2527
HhaI GCGC 1 cut(s) 668
Hin1II CATG 9 cut(s) 61, 153, 177, 183, 198, 1468, 2164, 2274, 3127
Hin6I GCGC 1 cut(s) 666
HinP1I GCGC 1 cut(s) 666
HindIII AAGCTT 3 cut(s) 1256, 1801, 2992
Hpy166II GTNNAC 4 cut(s) 1534, 2374, 2884, 3097
Hpy8I GTNNAC 4 cut(s) 1534, 2374, 2884, 3097
Hpy99I CGWCG 2 cut(s) 2248, 3106
HpyAV CCTTC 5 cut(s) 43, 348, 2326, 2675, 3121
HpyCH4III ACNGT 7 cut(s) 585, 932, 1513, 2125, 2140, 2421, 2525
Hsp92II CATG 9 cut(s) 61, 153, 177, 183, 198, 1468, 2164, 2274, 3127
HspAI GCGC 1 cut(s) 666
Kpn2I TCCGGA 2 cut(s) 772, 1716
Ksp22I TGATCA 1 cut(s) 717
Kzo9I GATC 9 cut(s) 693, 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
LguI GCTCTTC 1 cut(s) 1098
Lsp1109I GCAGC 9 cut(s) 680, 1422, 1606, 2249, 2449, 2532, 2593, 2596, 3051
LweI GCATC 4 cut(s) 277, 943, 1863, 3051
MaeI CTAG 6 cut(s) 416, 635, 1373, 1944, 2516, 3017
MaeIII GTNAC 6 cut(s) 1655, 1795, 1996, 2155, 2519, 2948
MalI GATC 9 cut(s) 695, 719, 969, 1242, 1271, 1602, 2644, 2912, 3045
MbiI CCGCTC 1 cut(s) 431
MboI GATC 9 cut(s) 693, 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
MflI RGATCY 1 cut(s) 2910
MhlI GDGCHC 4 cut(s) 566, 1230, 1536, 1829
MlsI TGGCCA 2 cut(s) 99, 348
MluNI TGGCCA 2 cut(s) 99, 348
MlyI GAGTC 3 cut(s) 149, 2066, 2447
MmeI TCCRAC 5 cut(s) 408, 480, 574, 604, 1506
Mox20I TGGCCA 2 cut(s) 99, 348
Mph1103I ATGCAT 1 cut(s) 898
MroI TCCGGA 2 cut(s) 772, 1716
MroXI GAANNNNTTC 3 cut(s) 1020, 1472, 2017
MscI TGGCCA 2 cut(s) 99, 348
MseI TTAA 9 cut(s) 74, 1382, 1652, 1755, 1923, 2132, 2915, 3156, 3223
MslI CAYNNNNRTG 5 cut(s) 56, 178, 2052, 2676, 2700
Msp20I TGGCCA 2 cut(s) 99, 348
MspA1I CMGCKG 4 cut(s) 671, 850, 917, 3005
MspCI CTTAAG 2 cut(s) 1651, 2914
MspR9I CCNGG 1 cut(s) 975
Mva1269I GAATGC 1 cut(s) 3179
MvaI CCWGG 1 cut(s) 975
NcoI CCATGG 2 cut(s) 2160, 2270
NdeI CATATG 3 cut(s) 816, 2032, 2208
NdeII GATC 9 cut(s) 693, 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
NlaIII CATG 9 cut(s) 61, 153, 177, 183, 198, 1468, 2164, 2274, 3127
NlaIV GGNNCC 4 cut(s) 328, 400, 1721, 1987
NmuCI GTSAC 1 cut(s) 2948
NsiI ATGCAT 1 cut(s) 898
NspI RCATGY 1 cut(s) 198
NspV TTCGAA 3 cut(s) 2024, 2528, 2997
OliI CACNNNNGTG 1 cut(s) 2676
PceI AGGCCT 3 cut(s) 639, 2744, 3036
PciSI GCTCTTC 1 cut(s) 1098
PctI GAATGC 1 cut(s) 3179
PdmI GAANNNNTTC 3 cut(s) 1020, 1472, 2017
PflMI CCANNNNNTGG 2 cut(s) 733, 2945
PleI GAGTC 3 cut(s) 149, 2065, 2447
PpsI GAGTC 3 cut(s) 149, 2065, 2447
PshBI ATTAAT 1 cut(s) 74
PsiI TTATAA 1 cut(s) 2556
Psp124BI GAGCTC 2 cut(s) 1230, 1829
Psp6I CCWGG 1 cut(s) 973
PspFI CCCAGC 3 cut(s) 850, 913, 1062
PspGI CCWGG 1 cut(s) 973
PspN4I GGNNCC 4 cut(s) 328, 400, 1721, 1987
PspPI GGNCC 7 cut(s) 4, 398, 542, 905, 977, 1769, 1891
PstI CTGCAG 4 cut(s) 1728, 2467, 2583, 3112
PstNI CAGNNNCTG 1 cut(s) 1074
PsuI RGATCY 1 cut(s) 2910
PvuII CAGCTG 3 cut(s) 850, 917, 3005
RsaI GTAC 6 cut(s) 1123, 1463, 1699, 2627, 2846, 2934
RsaNI GTAC 6 cut(s) 1122, 1462, 1698, 2626, 2845, 2933
RseI CAYNNNNRTG 5 cut(s) 56, 178, 2052, 2676, 2700
SacI GAGCTC 2 cut(s) 1230, 1829
SapI GCTCTTC 1 cut(s) 1098
SaqAI TTAA 9 cut(s) 74, 1382, 1652, 1755, 1923, 2132, 2915, 3156, 3223
Sau3AI GATC 9 cut(s) 693, 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
Sau96I GGNCC 7 cut(s) 4, 398, 542, 905, 977, 1769, 1891
SchI GAGTC 3 cut(s) 149, 2066, 2447
ScrFI CCNGG 1 cut(s) 975
SduI GDGCHC 4 cut(s) 566, 1230, 1536, 1829
SfaNI GCATC 4 cut(s) 277, 943, 1863, 3051
SfcI CTRYAG 5 cut(s) 1724, 2463, 2579, 2884, 3108
SfuI TTCGAA 3 cut(s) 2024, 2528, 2997
SgrAI CRCCGGYG 1 cut(s) 614
SinI GGWCC 5 cut(s) 4, 905, 977, 1769, 1891
SmiMI CAYNNNNRTG 5 cut(s) 56, 178, 2052, 2676, 2700
SmlI CTYRAG 5 cut(s) 1246, 1651, 1822, 2649, 2914
SmoI CTYRAG 5 cut(s) 1246, 1651, 1822, 2649, 2914
SpeI ACTAGT 1 cut(s) 1943
SseBI AGGCCT 3 cut(s) 639, 2744, 3036
SspI AATATT 3 cut(s) 134, 1183, 1963
SspMI CTAG 6 cut(s) 416, 635, 1373, 1944, 2516, 3017
SstI GAGCTC 2 cut(s) 1230, 1829
StuI AGGCCT 3 cut(s) 639, 2744, 3036
StyD4I CCNGG 1 cut(s) 973
StyI CCWWGG 6 cut(s) 1130, 1634, 2160, 2270, 2515, 3031
TaaI ACNGT 7 cut(s) 585, 932, 1513, 2125, 2140, 2421, 2525
TaqI TCGA 9 cut(s) 372, 951, 1014, 1268, 2024, 2528, 2807, 2997, 3011
TatI WGTACW 2 cut(s) 1461, 2932
TauI GCSGC 1 cut(s) 674
Tru1I TTAA 9 cut(s) 74, 1382, 1652, 1755, 1923, 2132, 2915, 3156, 3223
Tru9I TTAA 9 cut(s) 74, 1382, 1652, 1755, 1923, 2132, 2915, 3156, 3223
TscAI CASTG 6 cut(s) 738, 835, 1093, 1228, 1555, 1893
TseFI GTSAC 1 cut(s) 2948
TseI GCWGC 9 cut(s) 668, 1435, 1594, 2237, 2462, 2545, 2581, 2584, 3064
Tsp45I GTSAC 1 cut(s) 2948
TspGWI ACGGA 2 cut(s) 594, 2862
TspRI CASTG 6 cut(s) 738, 835, 1093, 1228, 1555, 1893
Van91I CCANNNNNTGG 2 cut(s) 733, 2945
Vha464I CTTAAG 2 cut(s) 1651, 2914
VneI GTGCAC 1 cut(s) 1532
VpaK11BI GGWCC 5 cut(s) 4, 905, 977, 1769, 1891
VspI ATTAAT 1 cut(s) 74
XapI RAATTY 5 cut(s) 1324, 1585, 1629, 2026, 2475
XceI RCATGY 1 cut(s) 198
XmaJI CCTAGG 1 cut(s) 2515
XmiI GTMKAC 2 cut(s) 2373, 2883
XmnI GAANNNNTTC 3 cut(s) 1020, 1472, 2017
XspI CTAG 6 cut(s) 416, 635, 1373, 1944, 2516, 3017
Zsp2I ATGCAT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.