Rmu_sc0000447.1_g000012
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000447.1
OG0014921
Physical Location & Seq
Forward (+)
72258 .. 75524
3267 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000447.1_g000012.1.cds

Sequence Viewer

Length: 3174 bp
atgtccaaactaaccttggtctcctctctgtcccctgatggccaagctcttctctcccttcttcctgcaaagcaatcttcctctgtactctcctcatggaacccatcaagccagacaccatgttcatggcaaggaatcacatgctcaccacaaaacagagtcatctccctctctctccccaacgttttcctcaatctctcctctttacccccacagctctcctctctcacatctctccagcttctcaatctctcctccaccaacatttcaggcactatcccaccttcctttggacaacttactcatcttcgcctcctcgacctctcctccaattctctgtttggtccgattcctccagaactaggcgctctctctgctctccagttcctcttcttgaactcaaacagattgtcagataaaatccctcaacaacttgccaacctcacttcacttcaagtcctttgtctgcaagacaatctcatcaacggctcgataccctcccaactcggttctttggcttccctccaacagtttcgtgttggtgggaatccatatataaccggagaaattccttcccagttgggactactcaccaatctcaccacattcggagctgcggctactggactttccgggactattccacccacatttggaaacttgatcaatcttcagacactggcgctctatgatactgaaatagttggttcaataccacccgaaatcgggctttgttctgagctgaggaacttgtacctgcacatgaacaagctcactggttccattcctccacaattaggtaagctgcaaaagctcaccagcctgcttgtttggggaaatgacttatcaggatcaatccccgctgagatttccaactgttcctcacttgttgttctcgatgcttctgcaaatgatctcactggagagattccaagagacttggggaagctggtgctccttgaacagcttcacctatcggataactcgcttacagggtcgattccatcgcagctcagcaactgtagcagcctaacagctctccagctcgataagaatcagttatcagggacaattccatggcaggttggtaatctgaaatacttgcagagtttcttcttgtggggcaatgcagtgtctggaactattcctgcctcgtttgggaactgcactgaactgtatgcacttgatctttcgagaaacaagctcaccgggtcaatcccagaagagattttcggtttgaagaaactgagcaagcttttgcttctgggaaattctttatccggcaggttgccacctagtgtagcaaaatgtcaatctctagtgcggctgagactcggtgagaaccagctttcaggacaaattccaaaggagataggccaactgcaaaaccttgtgttccttgacttgtacatgaatcatttctctggaggccttcccattgagattgccaacatcacagttcttgagctactggatgtgcacaataactacattggaggcgaaatcccatctcagtttggggagcttgtaaatttggagcagcttgatctgagccgaaacagcttcaagggtgagattcctgggagctttgggaacttgagttacttgaacaagctcatcatcaacaacaatcttctcacaggttcaataccaaagtcttttcgaaatttgcagaaactgacgttgttggatttgagcttcaacagcctctctggtgcaatcccatctgaaattggtcatgtgacaagcttgcaaatcagtttggatttgagttccaatggttttacaggagaacttccggagacaatggcggccttgacacagttacagtgtctggatctttcccataatatgctctttgggaaaattaaggttcttggttctctaactagtctcgcttcccttaatatctcctgcaatgatttctcaggtgctatcccaataaccaccccgttcttcagaactctctcctcaaattcttaccttcaaaatccacatatttgtgagtctaatgatggctctacttgctcttcaagtctaatgcggaagaatggtttgaaatcttcgaaaactattgctttagtttgtgtgattctctcttcagtaaccgtagcagttgttgcttcatggattcttgtgatgcgaaatcatagatatatggtagagaaatctttaggagcattagcatcctcgtcaggggctgaagatttctcctatccgtggactttcatcccattccagaagttcaatttcactattgacaacatcttggattgtttgaaagaagaaaatgtgattggaaaaggatgttctggggtcgtctacaaggcagaaatgcaaaccggagagctaattgcggtgaaaaagttgtggaagacaaagcgagaggaagaaccaatagactctttcgctgcagagattcaaattcttggacacattcgccaccggaacattgtgaagctcctagggtactgttcaaatagaagtgtcaagctacttctttacaatttcattcccaatggcaatctacaacaactattgcaagacaataggaacttggactgggaaactaggtacaagattgctattgggtcagctcagggtttggcataccttcatcatgattgtgtcccagcaattcttcatagggatgtgaagtgcaataacatattactagattccaaatgcgaagcttatcttgcagattttggtcttgcgaaattgatgaactccccaacttaccatcatgctatgtccagagttgctggctcttatggttacattgcaccagagtatggatacacaatgaacataacagagaagagtgatgtctacagctatggtgtggttttgctagagatcctaagcggacgtaatgccgttcagccccagattggtgatggactacacattgttgaatgggtgaagaagaagatgggaagtttcgaaccagctttatcagtactagatacaaagctccaaggcctgccagatcaaatggttcaagagatgctgcaaacacttggaatagcaatgttttgtgtcaactcttcgcccgcagaacgacccaccatgaaggaagtggtggcattgctaatggaggttaagagccaacctgaagaatggggaaaaacttctcaacccctaataaagcagtcttcaaatcaaagttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1057

Amino Acids

114.86

Weight (kDa)

6.31

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34110 AT1G34110
fragaria_vesca FvH4_2g13450
malus_domestica MD05G1035300.v1.1 MD10G1037600.v1.1
prunus_persica Prupe.8G043000_v2.0.a1
pyrus_communis pycom05g02560
rosa_chinensis RchiOBHm_Chr6g0275071
rosa_laevigata RLG00000013500
rosa_multiflora Rmu_sc0000447.1_g000012
rosa_roxburghii Rroxscaffold_7G00193690
rosa_rugosa Rorug06G0087900
rosa_samantha Rh6AG202200 Rh6BG205600 Rh6CG207500 Rh6DG197400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 765, 1072, 1275
AccB7I CCANNNNNTGG 1 cut(s) 2795
AccI GTMKAC 2 cut(s) 2322, 2832
AccIII TCCGGA 1 cut(s) 1798
AciI CCGC 8 cut(s) 617, 861, 1324, 1811, 2044, 2357, 2868, 3057
AclI AACGTT 1 cut(s) 183
AclWI GGATC 3 cut(s) 859, 1845, 2854
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 7 cut(s) 567, 1270, 1359, 1531, 1666, 1975, 2424
AcuI CTGAAG 5 cut(s) 656, 1942, 2085, 2223, 3138
AdeI CACNNNGTG 1 cut(s) 1298
AfaI GTAC 6 cut(s) 87, 755, 1409, 2471, 2576, 2964
AhlI ACTAGT 1 cut(s) 1889
AjnI CCWGG 1 cut(s) 1579
AjuI GAANNNNNNNTTGG 2 cut(s) 2280, 2312
Alw21I GWGCWC 2 cut(s) 957, 1482
Alw26I GTCTC 5 cut(s) 25, 930, 1324, 1796, 1898
Alw44I GTGCAC 1 cut(s) 1478
AlwI GGATC 3 cut(s) 859, 1845, 2854
AlwNI CAGNNNCTG 6 cut(s) 679, 1020, 1136, 1678, 1834, 2201
Aor13HI TCCGGA 1 cut(s) 1798
AoxI GGCC 5 cut(s) 40, 1375, 1429, 1812, 2983
ApaLI GTGCAC 1 cut(s) 1478
ApeKI GCWGC 7 cut(s) 614, 805, 1009, 1026, 1540, 2411, 3013
ApoI RAATTY 7 cut(s) 567, 1270, 1359, 1531, 1666, 1975, 2424
ArsI GACNNNNNNTTYG 2 cut(s) 395, 427
Asp700I GAANNNNTTC 3 cut(s) 966, 1418, 3133
AspA2I CCTAGG 1 cut(s) 2464
AspLEI GCGC 2 cut(s) 368, 685
AspS9I GGNCC 1 cut(s) 344
AsuC2I CCSGG 2 cut(s) 634, 1210
AsuII TTCGAA 3 cut(s) 1663, 2066, 2946
AvaII GGWCC 1 cut(s) 344
AvrII CCTAGG 1 cut(s) 2464
BaeGI GKGCMC 1 cut(s) 1482
BalI TGGCCA 1 cut(s) 42
BbsI GAAGAC 2 cut(s) 2381, 3150
Bbv12I GWGCWC 2 cut(s) 957, 1482
BbvCI CCTCAGC 1 cut(s) 743
BbvI GCAGC 7 cut(s) 601, 792, 1021, 1038, 1552, 2398, 3000
BccI CCATC 9 cut(s) 32, 112, 1012, 1516, 1732, 2009, 2751, 2894, 2929
BceAI ACGGC 2 cut(s) 502, 2864
BcgI CGANNNNNNTGC 2 cut(s) 887, 921
BciT130I CCWGG 1 cut(s) 1581
BciVI GTATCC 1 cut(s) 2792
BclI TGATCA 1 cut(s) 663
BcnI CCSGG 2 cut(s) 634, 1210
BcoDI GTCTC 5 cut(s) 25, 930, 1324, 1796, 1898
BcuI ACTAGT 1 cut(s) 1889
BfaI CTAG 9 cut(s) 362, 1296, 1319, 1890, 2465, 2571, 2675, 2855, 2966
BfmI CTRYAG 3 cut(s) 1021, 2412, 2833
BfoI RGCGCY 2 cut(s) 369, 686
BfuAI ACCTGC 3 cut(s) 765, 1072, 1275
BfuI GTATCC 1 cut(s) 2792
BlnI CCTAGG 1 cut(s) 2464
BlpI GCTNAGC 1 cut(s) 1013
BmcAI AGTACT 1 cut(s) 2964
Bme1390I CCNGG 3 cut(s) 634, 1210, 1581
Bme18I GGWCC 1 cut(s) 344
BmgT120I GGNCC 1 cut(s) 344
BmiI GGNNCC 2 cut(s) 101, 781
BmrFI CCNGG 3 cut(s) 634, 1210, 1581
BmrI ACTGGG 2 cut(s) 571, 2572
BmsI GCATC 4 cut(s) 889, 2130, 2195, 3000
BmuI ACTGGG 2 cut(s) 571, 2572
BpiI GAAGAC 2 cut(s) 2381, 3150
BpmI CTGGAG 6 cut(s) 221, 339, 365, 942, 1025, 1446
Bpu10I CCTNAGC 3 cut(s) 743, 2598, 2864
Bpu1102I GCTNAGC 1 cut(s) 1013
Bpu14I TTCGAA 3 cut(s) 1663, 2066, 2946
BpuEI CTTGAG 2 cut(s) 1484, 1618
BpuMI CCSGG 2 cut(s) 634, 1210
BsaBI GATNNNNATC 1 cut(s) 1053
BsaI GGTCTC 1 cut(s) 25
BsaJI CCNNGG 6 cut(s) 15, 1076, 1580, 2219, 2464, 2980
BsaWI WCCGGW 4 cut(s) 560, 1798, 2342, 2445
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bse1I ACTGG 8 cut(s) 382, 577, 628, 684, 781, 925, 1476, 2567
Bse3DI GCAATG 5 cut(s) 1132, 1924, 2781, 3039, 3089
Bse8I GATNNNNATC 1 cut(s) 1053
BseAI TCCGGA 1 cut(s) 1798
BseBI CCWGG 1 cut(s) 1581
BseDI CCNNGG 6 cut(s) 15, 1076, 1580, 2219, 2464, 2980
BseGI GGATG 5 cut(s) 1480, 2186, 2229, 2312, 2656
BseJI GATNNNNATC 1 cut(s) 1053
BseMI GCAATG 5 cut(s) 1132, 1924, 2781, 3039, 3089
BseNI ACTGG 8 cut(s) 382, 577, 628, 684, 781, 925, 1476, 2567
BseRI GAGGAG 8 cut(s) 13, 82, 190, 211, 244, 305, 316, 1960
BseSI GKGCMC 1 cut(s) 1482
BseXI GCAGC 7 cut(s) 601, 792, 1021, 1038, 1552, 2398, 3000
BseYI CCCAGC 1 cut(s) 2632
BsgI GTGCAG 2 cut(s) 743, 1150
BshFI GGCC 5 cut(s) 42, 1377, 1431, 1814, 2985
BsiHKAI GWGCWC 2 cut(s) 957, 1482
BsiSI CCGG 7 cut(s) 561, 633, 1209, 1281, 1799, 2343, 2446
BslFI GGGAC 5 cut(s) 16, 597, 649, 1081, 2615
BsmAI GTCTC 5 cut(s) 25, 930, 1324, 1796, 1898
BsmFI GGGAC 5 cut(s) 16, 597, 649, 1081, 2615
BsnI GGCC 5 cut(s) 42, 1377, 1431, 1814, 2985
Bso31I GGTCTC 1 cut(s) 25
Bsp119I TTCGAA 3 cut(s) 1663, 2066, 2946
Bsp1286I GDGCHC 2 cut(s) 957, 1482
Bsp13I TCCGGA 1 cut(s) 1798
Bsp1407I TGTACA 1 cut(s) 1407
Bsp143I GATC 8 cut(s) 663, 851, 913, 1186, 1546, 1837, 2859, 2992
Bsp1720I GCTNAGC 1 cut(s) 1013
Bsp19I CCATGG 1 cut(s) 1076
BspACI CCGC 8 cut(s) 617, 861, 1324, 1811, 2044, 2357, 2868, 3057
BspANI GGCC 5 cut(s) 42, 1377, 1431, 1814, 2985
BspEI TCCGGA 1 cut(s) 1798
BspHI TCATGA 1 cut(s) 2620
BspLI GGNNCC 2 cut(s) 101, 781
BspMAI CTGCAG 1 cut(s) 2416
BspMI ACCTGC 3 cut(s) 765, 1072, 1275
BspPI GGATC 3 cut(s) 859, 1845, 2854
BspQI GCTCTTC 2 cut(s) 54, 2035
BspT104I TTCGAA 3 cut(s) 1663, 2066, 2946
BspTNI GGTCTC 1 cut(s) 25
BsrDI GCAATG 5 cut(s) 1132, 1924, 2781, 3039, 3089
BsrGI TGTACA 1 cut(s) 1407
BsrI ACTGG 8 cut(s) 382, 577, 628, 684, 781, 925, 1476, 2567
BssECI CCNNGG 6 cut(s) 15, 1076, 1580, 2219, 2464, 2980
BssMI GATC 8 cut(s) 663, 851, 913, 1186, 1546, 1837, 2859, 2992
BssT1I CCWWGG 4 cut(s) 15, 1076, 2464, 2980
Bst2UI CCWGG 1 cut(s) 1581
Bst4CI ACNGT 9 cut(s) 531, 878, 1022, 1176, 1459, 1824, 1830, 2110, 2474
Bst6I CTCTTC 7 cut(s) 54, 395, 1218, 2035, 2104, 2816, 3055
BstAPI GCANNNNNTGC 1 cut(s) 2120
BstAUI TGTACA 1 cut(s) 1407
BstBI TTCGAA 3 cut(s) 1663, 2066, 2946
BstC8I GCNNGC 6 cut(s) 824, 1253, 1751, 2767, 2987, 3057
BstDSI CCRYGG 2 cut(s) 1076, 2219
BstF5I GGATG 5 cut(s) 1480, 2186, 2229, 2312, 2656
BstH2I RGCGCY 2 cut(s) 369, 686
BstHHI GCGC 2 cut(s) 368, 685
BstKTI GATC 8 cut(s) 666, 854, 916, 1189, 1549, 1840, 2862, 2995
BstMAI GTCTC 5 cut(s) 25, 930, 1324, 1796, 1898
BstMBI GATC 8 cut(s) 663, 851, 913, 1186, 1546, 1837, 2859, 2992
BstMWI GCNNNNNNNGC 8 cut(s) 374, 811, 1023, 1560, 1704, 2025, 2120, 2699
BstNI CCWGG 1 cut(s) 1581
BstNSI RCATGY 1 cut(s) 144
BstSCI CCNGG 3 cut(s) 632, 1208, 1579
BstSFI CTRYAG 3 cut(s) 1021, 2412, 2833
BstSLI GKGCMC 1 cut(s) 1482
BstV1I GCAGC 7 cut(s) 601, 792, 1021, 1038, 1552, 2398, 3000
BstV2I GAAGAC 2 cut(s) 2381, 3150
BstX2I RGATCY 2 cut(s) 1837, 2859
BstXI CCANNNNNNTGG 1 cut(s) 126
BstYI RGATCY 2 cut(s) 1837, 2859
BsuI GTATCC 1 cut(s) 2792
BsuRI GGCC 5 cut(s) 42, 1377, 1431, 1814, 2985
BtgI CCRYGG 2 cut(s) 1076, 2219
BtgZI GCGATG 1 cut(s) 990
BtsCI GGATG 5 cut(s) 1480, 2186, 2229, 2312, 2656
BtsI GCAGTG 1 cut(s) 1137
BtsIMutI CAGTG 6 cut(s) 677, 774, 918, 1137, 1167, 1835
BveI ACCTGC 3 cut(s) 765, 1072, 1275
Cac8I GCNNGC 6 cut(s) 824, 1253, 1751, 2767, 2987, 3057
CaiI CAGNNNCTG 6 cut(s) 679, 1020, 1136, 1678, 1834, 2201
CciI TCATGA 1 cut(s) 2620
CfoI GCGC 2 cut(s) 368, 685
Cfr13I GGNCC 1 cut(s) 344
Csp6I GTAC 6 cut(s) 86, 754, 1408, 2470, 2575, 2963
CviQI GTAC 6 cut(s) 86, 754, 1408, 2470, 2575, 2963
DpnI GATC 8 cut(s) 665, 853, 915, 1188, 1548, 1839, 2861, 2994
DpnII GATC 8 cut(s) 663, 851, 913, 1186, 1546, 1837, 2859, 2992
DraIII CACNNNGTG 1 cut(s) 1298
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 7 cut(s) 54, 395, 1218, 2035, 2104, 2816, 3055
EarI CTCTTC 7 cut(s) 54, 395, 1218, 2035, 2104, 2816, 3055
Eco130I CCWWGG 4 cut(s) 15, 1076, 2464, 2980
Eco147I AGGCCT 2 cut(s) 1431, 2985
Eco31I GGTCTC 1 cut(s) 25
Eco47I GGWCC 1 cut(s) 344
Eco57I CTGAAG 5 cut(s) 656, 1942, 2085, 2223, 3138
EcoRII CCWGG 1 cut(s) 1579
EcoT14I CCWWGG 4 cut(s) 15, 1076, 2464, 2980
ErhI CCWWGG 4 cut(s) 15, 1076, 2464, 2980
FalI AAGNNNNNCTT 2 cut(s) 2682, 2714
FaqI GGGAC 5 cut(s) 16, 597, 649, 1081, 2615
FauI CCCGC 2 cut(s) 868, 3064
FbaI TGATCA 1 cut(s) 663
FblI GTMKAC 2 cut(s) 2322, 2832
FokI GGATG 5 cut(s) 1487, 2173, 2216, 2319, 2663
FspBI CTAG 9 cut(s) 362, 1296, 1319, 1890, 2465, 2571, 2675, 2855, 2966
GlaI GCGC 2 cut(s) 367, 684
GsaI CCCAGC 1 cut(s) 2636
GsuI CTGGAG 6 cut(s) 221, 339, 365, 942, 1025, 1446
HaeII RGCGCY 2 cut(s) 369, 686
HaeIII GGCC 5 cut(s) 42, 1377, 1431, 1814, 2985
HapII CCGG 7 cut(s) 561, 633, 1209, 1281, 1799, 2343, 2446
HhaI GCGC 2 cut(s) 368, 685
Hin6I GCGC 2 cut(s) 366, 683
HinP1I GCGC 2 cut(s) 366, 683
HincII GTYRAC 1 cut(s) 3046
HindII GTYRAC 1 cut(s) 3046
HindIII AAGCTT 3 cut(s) 1253, 1747, 2691
HpaII CCGG 7 cut(s) 561, 633, 1209, 1281, 1799, 2343, 2446
Hpy166II GTNNAC 5 cut(s) 1480, 2223, 2323, 2833, 3046
Hpy8I GTNNAC 5 cut(s) 1480, 2223, 2323, 2833, 3046
HpyAV CCTTC 7 cut(s) 68, 294, 582, 1442, 1994, 2624, 3070
HpyCH4III ACNGT 9 cut(s) 531, 878, 1022, 1176, 1459, 1824, 1830, 2110, 2474
HpyCH4IV ACGT 3 cut(s) 183, 1682, 2872
HpyF10VI GCNNNNNNNGC 8 cut(s) 374, 811, 1023, 1560, 1704, 2025, 2120, 2699
HpySE526I ACGT 3 cut(s) 183, 1682, 2872
HspAI GCGC 2 cut(s) 366, 683
Kpn2I TCCGGA 1 cut(s) 1798
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 8 cut(s) 663, 851, 913, 1186, 1546, 1837, 2859, 2992
LguI GCTCTTC 2 cut(s) 54, 2035
LmnI GCTCC 8 cut(s) 611, 960, 1522, 1537, 1584, 2177, 2466, 2982
Lsp1109I GCAGC 7 cut(s) 601, 792, 1021, 1038, 1552, 2398, 3000
LweI GCATC 4 cut(s) 889, 2130, 2195, 3000
MaeI CTAG 9 cut(s) 362, 1296, 1319, 1890, 2465, 2571, 2675, 2855, 2966
MaeII ACGT 3 cut(s) 183, 1682, 2872
MaeIII GTNAC 5 cut(s) 1601, 1741, 1824, 2104, 2777
MalI GATC 8 cut(s) 665, 853, 915, 1188, 1548, 1839, 2861, 2994
MboI GATC 8 cut(s) 663, 851, 913, 1186, 1546, 1837, 2859, 2992
MflI RGATCY 2 cut(s) 1837, 2859
MhlI GDGCHC 2 cut(s) 957, 1482
MlsI TGGCCA 1 cut(s) 42
MluNI TGGCCA 1 cut(s) 42
MlyI GAGTC 4 cut(s) 168, 1326, 2015, 2396
MmeI TCCRAC 3 cut(s) 550, 897, 1668
Mox20I TGGCCA 1 cut(s) 42
MroI TCCGGA 1 cut(s) 1798
MroXI GAANNNNTTC 3 cut(s) 966, 1418, 3133
MscI TGGCCA 1 cut(s) 42
MseI TTAA 4 cut(s) 1869, 1905, 3105, 3172
MslI CAYNNNNRTG 4 cut(s) 124, 2001, 2625, 2649
Msp20I TGGCCA 1 cut(s) 42
MspA1I CMGCKG 1 cut(s) 863
MspI CCGG 7 cut(s) 561, 633, 1209, 1281, 1799, 2343, 2446
MspR9I CCNGG 3 cut(s) 634, 1210, 1581
MvaI CCWGG 1 cut(s) 1581
MwoI GCNNNNNNNGC 8 cut(s) 374, 811, 1023, 1560, 1704, 2025, 2120, 2699
NciI CCSGG 2 cut(s) 634, 1210
NcoI CCATGG 1 cut(s) 1076
NdeII GATC 8 cut(s) 663, 851, 913, 1186, 1546, 1837, 2859, 2992
NlaIV GGNNCC 2 cut(s) 101, 781
NmuCI GTSAC 1 cut(s) 1741
NspI RCATGY 1 cut(s) 144
NspV TTCGAA 3 cut(s) 1663, 2066, 2946
PagI TCATGA 1 cut(s) 2620
PceI AGGCCT 2 cut(s) 1431, 2985
PciSI GCTCTTC 2 cut(s) 54, 2035
PdmI GAANNNNTTC 3 cut(s) 966, 1418, 3133
PflMI CCANNNNNTGG 1 cut(s) 2795
PfoI TCCNGGA 1 cut(s) 632
PleI GAGTC 4 cut(s) 167, 1326, 2014, 2396
PpsI GAGTC 4 cut(s) 167, 1326, 2014, 2396
Psp1406I AACGTT 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 1579
PspFI CCCAGC 1 cut(s) 2632
PspGI CCWGG 1 cut(s) 1579
PspN4I GGNNCC 2 cut(s) 101, 781
PspPI GGNCC 1 cut(s) 344
PsrI GAACNNNNNNTAC 2 cut(s) 1586, 1618
PstI CTGCAG 1 cut(s) 2416
PstNI CAGNNNCTG 6 cut(s) 679, 1020, 1136, 1678, 1834, 2201
PsuI RGATCY 2 cut(s) 1837, 2859
RsaI GTAC 6 cut(s) 87, 755, 1409, 2471, 2576, 2964
RsaNI GTAC 6 cut(s) 86, 754, 1408, 2470, 2575, 2963
RseI CAYNNNNRTG 4 cut(s) 124, 2001, 2625, 2649
SapI GCTCTTC 2 cut(s) 54, 2035
SaqAI TTAA 4 cut(s) 1869, 1905, 3105, 3172
Sau3AI GATC 8 cut(s) 663, 851, 913, 1186, 1546, 1837, 2859, 2992
Sau96I GGNCC 1 cut(s) 344
ScaI AGTACT 1 cut(s) 2964
SchI GAGTC 4 cut(s) 168, 1326, 2015, 2396
ScrFI CCNGG 3 cut(s) 634, 1210, 1581
SduI GDGCHC 2 cut(s) 957, 1482
SfaNI GCATC 4 cut(s) 889, 2130, 2195, 3000
SfcI CTRYAG 3 cut(s) 1021, 2412, 2833
SfuI TTCGAA 3 cut(s) 1663, 2066, 2946
SinI GGWCC 1 cut(s) 344
SmiMI CAYNNNNRTG 4 cut(s) 124, 2001, 2625, 2649
SmlI CTYRAG 2 cut(s) 1463, 1597
SmoI CTYRAG 2 cut(s) 1463, 1597
SpeI ACTAGT 1 cut(s) 1889
SseBI AGGCCT 2 cut(s) 1431, 2985
SsiI CCGC 8 cut(s) 617, 861, 1324, 1811, 2044, 2357, 2868, 3057
SspMI CTAG 9 cut(s) 362, 1296, 1319, 1890, 2465, 2571, 2675, 2855, 2966
StuI AGGCCT 2 cut(s) 1431, 2985
StyD4I CCNGG 3 cut(s) 632, 1208, 1579
StyI CCWWGG 4 cut(s) 15, 1076, 2464, 2980
TaaI ACNGT 9 cut(s) 531, 878, 1022, 1176, 1459, 1824, 1830, 2110, 2474
TaiI ACGT 3 cut(s) 186, 1685, 2875
TaqI TCGA 9 cut(s) 318, 491, 897, 998, 1047, 1193, 1663, 2066, 2946
TatI WGTACW 3 cut(s) 85, 1407, 2962
TauI GCSGC 3 cut(s) 620, 1327, 1814
Tru1I TTAA 4 cut(s) 1869, 1905, 3105, 3172
Tru9I TTAA 4 cut(s) 1869, 1905, 3105, 3172
TscAI CASTG 6 cut(s) 684, 781, 925, 1137, 1174, 1835
TseFI GTSAC 1 cut(s) 1741
TseI GCWGC 7 cut(s) 614, 805, 1009, 1026, 1540, 2411, 3013
Tsp45I GTSAC 1 cut(s) 1741
TspGWI ACGGA 1 cut(s) 2208
TspRI CASTG 6 cut(s) 684, 781, 925, 1137, 1174, 1835
Van91I CCANNNNNTGG 1 cut(s) 2795
VneI GTGCAC 1 cut(s) 1478
VpaK11BI GGWCC 1 cut(s) 344
XapI RAATTY 7 cut(s) 567, 1270, 1359, 1531, 1666, 1975, 2424
XceI RCATGY 1 cut(s) 144
XcmI CCANNNNNNNNNTGG 3 cut(s) 13, 2897, 3079
XmaJI CCTAGG 1 cut(s) 2464
XmiI GTMKAC 2 cut(s) 2322, 2832
XmnI GAANNNNTTC 3 cut(s) 966, 1418, 3133
XspI CTAG 9 cut(s) 362, 1296, 1319, 1890, 2465, 2571, 2675, 2855, 2966
ZrmI AGTACT 1 cut(s) 2964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.