Rh6BG205600
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0014921
Physical Location & Seq
Forward (+)
38724718 .. 38728047
3330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG205600.1

Sequence Viewer

Length: 2868 bp
ATGGACAACCAAAGACTCACCAGTACTACTTTTTCCTTCACATTGTTCATCATTTTCATAACAATGTCCAAACTAACCTTGGTCTCCTCTCTGTCCCCTGATGGCCAAGCTCTTCTCTCCCTTCTTCCTGCAAAGCAATCTTTCTCTGTACTCTCCTCATGGAACCCATCAAGCCAGACACCATGTTCCTGGCAAGGAATCACATGCTCACCACAAAACAGAGTCATCTCCCTCTCTCTCCCCAACGTTTTCCTCAATCTCTCCTCTTTACCCCCACAGCTCTCCTCTCTCTCATCTCTCCAGCTTCTCAATCTCTCCTCCACCAACATTTCAGGCACTATCCCATCTTCCTTTGGACAACTTACTCATCTTCGCCTCCTCGACCTCTCCTCCAACTCTCTGTTAGGTCCGATTCCTCCAGAACTAGGCGCTCTCTCTGCTCTCCAGTTCCTCTTCTTGAACTCAAACAGATTGTCAGATAAAATCCCTCAACAACTTGCCAACCTCACTTCACTTCAAGTCCTTTGTCTCCAAGACAATCTCATCAACGGCTCGATACCCTCCCAACTCGGTTCTTTGGCTTCCCTCCAACAGTTTCGTGTTGGTGGGAATCCATATATAACCGGAGAAATTCCTTCCCAGTTGGGACTACTCACCAATCTCACCACATTCGGAGCTGCGGCTACTGGACTTTCCGGGACTATTCCACCCACATTTGGAAACTTGATCAATCTTCAGACACTGGCGCTCTATGATACTGAAATAGTTGGTTCAATACCACCCGAAATCGGGCTTTGTTCTGAGCTGAGGAACTTGTACCTGCACATGAACAAGCTCACTGGTTCCATTCCTCCACAATTAGGTAAGCTGCAAAAGCTCACCAGCCTGCTTGTTTGGGGAAATGACTTATCAGGATCAATCCCCGCTGAGATTTCCAACTGTTCCTCACTTGTTGTTCTCGATGCTTCTGCAAATGATCTCACTGGAGAGATTCCAAGAGACTTGGGGAAGCTGGTGCTCCTTGAACAGCTTCACCTATCGGATAACTCGCTTACAGGGTCGATTCCATCGCAGCTCAGCAACTGTAGCAGCCTTACAGCTCTCCAGCTCGACAAGAATCAGTTATCAGGGACAATTCCATGGCAGGTTGGTAATCTGAAATACTTGCAGAGTTTCTTCTTGTGGGGCAATGCAGTGTCTGGAACTATTCCTGCCTCGTTTGGGAACTGCACTGAACTGTATGCACTTGATCTTTCGAGAAACAAGCTCACCGGGTCAATACCAGAAGAGATTTTCGGTTTGAAGAAACTGAGCAAGCTTTTGCTTCTGGGAAATTCTTTGTCCGGCAGGTTGCCACCTAGTGTAGCAAAATGTCAATCTCTAGTGCGGCTGAGACTCGGTGAGAACCAGCTTTCAGGACAAATTCCAAAGGAGATAGGCCAACTGCAAAACCTTGTGTTCCTTGACTTGTACATGAATCATTTCTCTGGAGGCCTTCCCATTGAGATTGCCAACATCACAGTTCTTGAGCTACTGGATGTGCACAATAACTACATTGGAGGCGAAATCCCATCTCAGTTTGGGGAGCTTGTAAATTTGGAGCAGCTTGATCTGAGCCGAAACAGCTTCAAGGGTGAGATTCCTTGGAGCTTTGGGAACTTGAGTTACTTGAACAAGCTCATCATCAACAACAATCTTCTCACAGGTTCAATACCAAAGTCTTTTCGAAATTTGCAGAAACTGACGTTGTTGGATTTGAGCTTCAACAGCCTCTCTGGTGCAATCCCATCTGAAATTGGTCATGTGACAAGCTTGCAAATCAGTTTGGATTTGAGTTCCAATGGTTTTACCGGAGAACTTCCGGAGACAATGGCGGCTTTGACACAGTTACAGTGTCTGGATCTTTCCCATAATATGCTCTTTGGGAAAATTAAGGTTCTTGGTTCTCTAACTAGTCTCGCTTCCCTTAATATCTCCTGCAATGATTTCTCAGGTGCTATCCCAATAACCACCCCGTTCTTCAGAACTCTCTCCTCAAATTCTTACCTTCAAAATCCACATATTTGTGAGTCTAATGATGGCTCTACTTGCTCTTCAAGTCTAATGCGGAAGAATGGTTTGAAATCTTCGAAAACTATTGCTTTAATTTGTGTGATTCTCTCTTCAGTAACCATAGCAGTTGTTGCTTCATGGATTCTTGTGATGCGAAATCATAGATATATGGTAGAGAAATCTTTAGGAGCATTAGCATCCTCGTCAGGGGCTGAAGATTTCTCCTATCCGTGGACTTTCATCCCATTCCAGAAGTTCAATTTCACTATTGACAACATCTTGGATTGTTTGAAAGAAGAAAATGTGATTGGAAAAGGATGTTCTGGGGTCGTCTACAAGGCAGAAATGCAAACCGGAGAGCTAATTGCGGTGAAAAAGTTGTGGAAGACAAAGCGAGAGGAAGAACCAATAGACTCTTTCGCTGCAGAGATTCAAATTCTTGGACACATTCGCCACCGGAACATTGTGAAGCTCCTAGGCTATGGTGTGGTTTTGCTAGAGATCCTAAGCGGACGTAATGCCGTTCAGCCCCAGATTGGTGATGGACTACACATTGTTGAATGGGTGAAGAAGAAGATGGGAAGTTTCGAACCAGCTTTATCAGTACTAGATACAAAGCTCCAAGGCCTGCCAGATCAAATGGTTCAAGAGATGCTGCAAACACTTGGAATAGCAATGTTTTGTGTCAACTCTTCGCCGGCGGAACGACCCACCATGAAGGAAGTGGTGGCATTGCTAATGGAGGTTAAGAGCCAACCTGAAGAATGGGGAAAAACTTCTCAACCCCTAATAAAGCAGTCTTCAAATCAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

955

Amino Acids

103.53

Weight (kDa)

6.12

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 32 - 70 3.2e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 85 - 224 6e-13 Leucine-rich repeat region
LRR_14 PF23598 233 - 323 7.9e-06 Leucine-rich repeat region
LRR_8 PF13855 389 - 447 1.7e-07 Leucine rich repeat
LRR_14 PF23598 503 - 586 1.5e-06 Leucine-rich repeat region
LRR_8 PF13855 556 - 615 4.9e-06 Leucine rich repeat
LRR_14 PF23598 594 - 660 2.5e-07 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 782 - 845 1.7e-07 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 783 - 844 4.5e-09 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34110 AT1G34110
fragaria_vesca FvH4_2g13450
malus_domestica MD05G1035300.v1.1 MD10G1037600.v1.1
prunus_persica Prupe.8G043000_v2.0.a1
pyrus_communis pycom05g02560
rosa_chinensis RchiOBHm_Chr6g0275071
rosa_laevigata RLG00000013500
rosa_multiflora Rmu_sc0000447.1_g000012
rosa_roxburghii Rroxscaffold_7G00193690
rosa_rugosa Rorug06G0087900
rosa_samantha Rh6AG202200 Rh6BG205600 Rh6CG207500 Rh6DG197400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 828, 1135, 1338
AccI GTMKAC 1 cut(s) 2385
AccIII TCCGGA 1 cut(s) 1861
AciI CCGC 8 cut(s) 680, 924, 1387, 1874, 2107, 2420, 2562, 2753
AclI AACGTT 1 cut(s) 246
AclWI GGATC 3 cut(s) 922, 1908, 2548
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 7 cut(s) 630, 1333, 1422, 1594, 1729, 2038, 2487
AcuI CTGAAG 5 cut(s) 719, 2005, 2148, 2286, 2832
AdeI CACNNNGTG 1 cut(s) 1361
AfaI GTAC 5 cut(s) 25, 150, 818, 1472, 2658
AfiI CCNNNNNNNGG 9 cut(s) 425, 716, 788, 789, 860, 1221, 2259, 2283, 2588
AhlI ACTAGT 1 cut(s) 1952
AjnI CCWGG 1 cut(s) 188
AjuI GAANNNNNNNTTGG 2 cut(s) 2343, 2375
Alw21I GWGCWC 2 cut(s) 1020, 1545
Alw26I GTCTC 6 cut(s) 88, 533, 993, 1387, 1859, 1961
Alw44I GTGCAC 1 cut(s) 1541
AlwI GGATC 3 cut(s) 922, 1908, 2548
AlwNI CAGNNNCTG 6 cut(s) 742, 1083, 1199, 1741, 1897, 2264
Aor13HI TCCGGA 1 cut(s) 1861
AoxI GGCC 4 cut(s) 103, 1438, 1492, 2677
ApaLI GTGCAC 1 cut(s) 1541
ApeKI GCWGC 7 cut(s) 677, 868, 1072, 1089, 1603, 2474, 2707
ApoI RAATTY 7 cut(s) 630, 1333, 1422, 1594, 1729, 2038, 2487
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
Asp700I GAANNNNTTC 3 cut(s) 1029, 1481, 2827
AspA2I CCTAGG 1 cut(s) 2527
AspLEI GCGC 2 cut(s) 431, 748
AspS9I GGNCC 1 cut(s) 407
AsuC2I CCSGG 2 cut(s) 697, 1273
AsuII TTCGAA 3 cut(s) 1726, 2129, 2640
AvaII GGWCC 1 cut(s) 407
AvrII CCTAGG 1 cut(s) 2527
BaeGI GKGCMC 1 cut(s) 1545
BalI TGGCCA 1 cut(s) 105
BbsI GAAGAC 2 cut(s) 2444, 2844
Bbv12I GWGCWC 2 cut(s) 1020, 1545
BbvCI CCTCAGC 1 cut(s) 806
BbvI GCAGC 7 cut(s) 664, 855, 1084, 1101, 1615, 2461, 2694
BccI CCATC 9 cut(s) 95, 175, 352, 1075, 1579, 1795, 2072, 2588, 2623
BceAI ACGGC 2 cut(s) 565, 2558
BcgI CGANNNNNNTGC 2 cut(s) 950, 984
BciT130I CCWGG 1 cut(s) 190
BclI TGATCA 1 cut(s) 726
BcnI CCSGG 2 cut(s) 697, 1273
BcoDI GTCTC 6 cut(s) 88, 533, 993, 1387, 1859, 1961
BcuI ACTAGT 1 cut(s) 1952
BfaI CTAG 7 cut(s) 425, 1359, 1382, 1953, 2528, 2549, 2660
BfmI CTRYAG 2 cut(s) 1084, 2475
BfoI RGCGCY 2 cut(s) 432, 749
BfuAI ACCTGC 3 cut(s) 828, 1135, 1338
BlnI CCTAGG 1 cut(s) 2527
BlpI GCTNAGC 1 cut(s) 1076
BmcAI AGTACT 2 cut(s) 25, 2658
Bme1390I CCNGG 3 cut(s) 190, 697, 1273
Bme18I GGWCC 1 cut(s) 407
BmgT120I GGNCC 1 cut(s) 407
BmiI GGNNCC 2 cut(s) 164, 844
BmrFI CCNGG 3 cut(s) 190, 697, 1273
BmrI ACTGGG 1 cut(s) 634
BmsI GCATC 4 cut(s) 952, 2193, 2258, 2694
BmuI ACTGGG 1 cut(s) 634
BpiI GAAGAC 2 cut(s) 2444, 2844
BpmI CTGGAG 6 cut(s) 284, 402, 428, 1005, 1088, 1509
Bpu10I CCTNAGC 2 cut(s) 806, 2558
Bpu1102I GCTNAGC 1 cut(s) 1076
Bpu14I TTCGAA 3 cut(s) 1726, 2129, 2640
BpuEI CTTGAG 2 cut(s) 1547, 1681
BpuMI CCSGG 2 cut(s) 697, 1273
BsaI GGTCTC 1 cut(s) 88
BsaJI CCNNGG 6 cut(s) 78, 1139, 1643, 2282, 2527, 2674
BsaWI WCCGGW 5 cut(s) 623, 1850, 1861, 2405, 2508
BsaXI ACNNNNNCTCC 2 cut(s) 374, 404
Bsc4I CCNNNNNNNGG 9 cut(s) 425, 716, 788, 789, 860, 1221, 2259, 2283, 2588
Bse118I RCCGGY 1 cut(s) 2749
Bse1I ACTGG 8 cut(s) 21, 445, 640, 691, 747, 844, 988, 1539
Bse3DI GCAATG 4 cut(s) 1195, 1987, 2733, 2783
BseAI TCCGGA 1 cut(s) 1861
BseBI CCWGG 1 cut(s) 190
BseDI CCNNGG 6 cut(s) 78, 1139, 1643, 2282, 2527, 2674
BseGI GGATG 4 cut(s) 1543, 2249, 2292, 2375
BseLI CCNNNNNNNGG 9 cut(s) 425, 716, 788, 789, 860, 1221, 2259, 2283, 2588
BseMI GCAATG 4 cut(s) 1195, 1987, 2733, 2783
BseMII CTCAG 9 cut(s) 792, 797, 918, 1090, 1301, 1382, 1589, 1604, 2004
BseNI ACTGG 8 cut(s) 21, 445, 640, 691, 747, 844, 988, 1539
BseRI GAGGAG 8 cut(s) 76, 145, 253, 274, 307, 368, 379, 2023
BseSI GKGCMC 1 cut(s) 1545
BseXI GCAGC 7 cut(s) 664, 855, 1084, 1101, 1615, 2461, 2694
BsgI GTGCAG 2 cut(s) 806, 1213
BshFI GGCC 4 cut(s) 105, 1440, 1494, 2679
BsiHKAI GWGCWC 2 cut(s) 1020, 1545
BsiSI CCGG 9 cut(s) 624, 696, 1272, 1344, 1851, 1862, 2406, 2509, 2750
BslFI GGGAC 4 cut(s) 79, 660, 712, 1144
BslI CCNNNNNNNGG 9 cut(s) 425, 716, 788, 789, 860, 1221, 2259, 2283, 2588
BsmAI GTCTC 6 cut(s) 88, 533, 993, 1387, 1859, 1961
BsmFI GGGAC 4 cut(s) 79, 660, 712, 1144
BsnI GGCC 4 cut(s) 105, 1440, 1494, 2679
Bso31I GGTCTC 1 cut(s) 88
Bsp119I TTCGAA 3 cut(s) 1726, 2129, 2640
Bsp1286I GDGCHC 2 cut(s) 1020, 1545
Bsp13I TCCGGA 1 cut(s) 1861
Bsp1407I TGTACA 1 cut(s) 1470
Bsp143I GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2553, 2686
Bsp1720I GCTNAGC 1 cut(s) 1076
Bsp19I CCATGG 1 cut(s) 1139
BspACI CCGC 8 cut(s) 680, 924, 1387, 1874, 2107, 2420, 2562, 2753
BspANI GGCC 4 cut(s) 105, 1440, 1494, 2679
BspCNI CTCAG 9 cut(s) 793, 798, 919, 1089, 1302, 1383, 1588, 1605, 2003
BspEI TCCGGA 1 cut(s) 1861
BspLI GGNNCC 2 cut(s) 164, 844
BspMAI CTGCAG 1 cut(s) 2479
BspMI ACCTGC 3 cut(s) 828, 1135, 1338
BspPI GGATC 3 cut(s) 922, 1908, 2548
BspQI GCTCTTC 2 cut(s) 117, 2098
BspT104I TTCGAA 3 cut(s) 1726, 2129, 2640
BspTNI GGTCTC 1 cut(s) 88
BsrDI GCAATG 4 cut(s) 1195, 1987, 2733, 2783
BsrFI RCCGGY 1 cut(s) 2749
BsrGI TGTACA 1 cut(s) 1470
BsrI ACTGG 8 cut(s) 21, 445, 640, 691, 747, 844, 988, 1539
BssAI RCCGGY 1 cut(s) 2749
BssECI CCNNGG 6 cut(s) 78, 1139, 1643, 2282, 2527, 2674
BssMI GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2553, 2686
BssT1I CCWWGG 5 cut(s) 78, 1139, 1643, 2527, 2674
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 7 cut(s) 594, 941, 1085, 1239, 1522, 1887, 1893
Bst6I CTCTTC 6 cut(s) 117, 458, 1281, 2098, 2167, 2749
BstAPI GCANNNNNTGC 1 cut(s) 2183
BstAUI TGTACA 1 cut(s) 1470
BstBI TTCGAA 3 cut(s) 1726, 2129, 2640
BstC8I GCNNGC 5 cut(s) 887, 1316, 1814, 2681, 2751
BstDSI CCRYGG 2 cut(s) 1139, 2282
BstF5I GGATG 4 cut(s) 1543, 2249, 2292, 2375
BstH2I RGCGCY 2 cut(s) 432, 749
BstHHI GCGC 2 cut(s) 431, 748
BstKTI GATC 8 cut(s) 729, 917, 979, 1252, 1612, 1903, 2556, 2689
BstMAI GTCTC 6 cut(s) 88, 533, 993, 1387, 1859, 1961
BstMBI GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2553, 2686
BstMWI GCNNNNNNNGC 7 cut(s) 437, 874, 1086, 1623, 1767, 2088, 2183
BstNI CCWGG 1 cut(s) 190
BstNSI RCATGY 1 cut(s) 207
BstSCI CCNGG 3 cut(s) 188, 695, 1271
BstSFI CTRYAG 2 cut(s) 1084, 2475
BstSLI GKGCMC 1 cut(s) 1545
BstV1I GCAGC 7 cut(s) 664, 855, 1084, 1101, 1615, 2461, 2694
BstV2I GAAGAC 2 cut(s) 2444, 2844
BstX2I RGATCY 2 cut(s) 1900, 2553
BstXI CCANNNNNNTGG 1 cut(s) 189
BstYI RGATCY 2 cut(s) 1900, 2553
BsuRI GGCC 4 cut(s) 105, 1440, 1494, 2679
BtgI CCRYGG 2 cut(s) 1139, 2282
BtgZI GCGATG 1 cut(s) 1053
BtsCI GGATG 4 cut(s) 1543, 2249, 2292, 2375
BtsI GCAGTG 1 cut(s) 1200
BtsIMutI CAGTG 6 cut(s) 740, 837, 981, 1200, 1230, 1898
BveI ACCTGC 3 cut(s) 828, 1135, 1338
Cac8I GCNNGC 5 cut(s) 887, 1316, 1814, 2681, 2751
CaiI CAGNNNCTG 6 cut(s) 742, 1083, 1199, 1741, 1897, 2264
CfoI GCGC 2 cut(s) 431, 748
Cfr10I RCCGGY 1 cut(s) 2749
Cfr13I GGNCC 1 cut(s) 407
Csp6I GTAC 5 cut(s) 24, 149, 817, 1471, 2657
CviAII CATG 9 cut(s) 159, 183, 204, 826, 1140, 1474, 1802, 2190, 2767
CviQI GTAC 5 cut(s) 24, 149, 817, 1471, 2657
DpnI GATC 8 cut(s) 728, 916, 978, 1251, 1611, 1902, 2555, 2688
DpnII GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2553, 2686
DraIII CACNNNGTG 1 cut(s) 1361
EaeI YGGCCR 1 cut(s) 103
Eam1104I CTCTTC 6 cut(s) 117, 458, 1281, 2098, 2167, 2749
EarI CTCTTC 6 cut(s) 117, 458, 1281, 2098, 2167, 2749
EciI GGCGGA 1 cut(s) 2768
Eco130I CCWWGG 5 cut(s) 78, 1139, 1643, 2527, 2674
Eco147I AGGCCT 2 cut(s) 1494, 2679
Eco31I GGTCTC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 407
Eco57I CTGAAG 5 cut(s) 719, 2005, 2148, 2286, 2832
EcoRII CCWGG 1 cut(s) 188
EcoT14I CCWWGG 5 cut(s) 78, 1139, 1643, 2527, 2674
ErhI CCWWGG 5 cut(s) 78, 1139, 1643, 2527, 2674
FaeI CATG 9 cut(s) 162, 186, 207, 829, 1143, 1477, 1805, 2193, 2770
FaqI GGGAC 4 cut(s) 79, 660, 712, 1144
FatI CATG 9 cut(s) 158, 182, 203, 825, 1139, 1473, 1801, 2189, 2766
FauI CCCGC 1 cut(s) 931
FbaI TGATCA 1 cut(s) 726
FblI GTMKAC 1 cut(s) 2385
FokI GGATG 4 cut(s) 1550, 2236, 2279, 2382
FspBI CTAG 7 cut(s) 425, 1359, 1382, 1953, 2528, 2549, 2660
GlaI GCGC 2 cut(s) 430, 747
GsuI CTGGAG 6 cut(s) 284, 402, 428, 1005, 1088, 1509
HaeII RGCGCY 2 cut(s) 432, 749
HaeIII GGCC 4 cut(s) 105, 1440, 1494, 2679
HapII CCGG 9 cut(s) 624, 696, 1272, 1344, 1851, 1862, 2406, 2509, 2750
HhaI GCGC 2 cut(s) 431, 748
Hin1II CATG 9 cut(s) 162, 186, 207, 829, 1143, 1477, 1805, 2193, 2770
Hin6I GCGC 2 cut(s) 429, 746
HinP1I GCGC 2 cut(s) 429, 746
HincII GTYRAC 1 cut(s) 2740
HindII GTYRAC 1 cut(s) 2740
HindIII AAGCTT 2 cut(s) 1316, 1810
HpaII CCGG 9 cut(s) 624, 696, 1272, 1344, 1851, 1862, 2406, 2509, 2750
Hpy166II GTNNAC 4 cut(s) 1543, 2286, 2386, 2740
Hpy8I GTNNAC 4 cut(s) 1543, 2286, 2386, 2740
HpyAV CCTTC 6 cut(s) 46, 131, 645, 1505, 2057, 2764
HpyCH4III ACNGT 7 cut(s) 594, 941, 1085, 1239, 1522, 1887, 1893
HpyCH4IV ACGT 3 cut(s) 246, 1745, 2566
HpyF10VI GCNNNNNNNGC 7 cut(s) 437, 874, 1086, 1623, 1767, 2088, 2183
HpySE526I ACGT 3 cut(s) 246, 1745, 2566
Hsp92II CATG 9 cut(s) 162, 186, 207, 829, 1143, 1477, 1805, 2193, 2770
HspAI GCGC 2 cut(s) 429, 746
Kpn2I TCCGGA 1 cut(s) 1861
KroI GCCGGC 1 cut(s) 2749
KroNI GCCGGC 1 cut(s) 2751
Ksp22I TGATCA 1 cut(s) 726
Kzo9I GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2553, 2686
LguI GCTCTTC 2 cut(s) 117, 2098
LmnI GCTCC 8 cut(s) 674, 1023, 1585, 1600, 1647, 2240, 2529, 2676
Lsp1109I GCAGC 7 cut(s) 664, 855, 1084, 1101, 1615, 2461, 2694
LweI GCATC 4 cut(s) 952, 2193, 2258, 2694
MaeI CTAG 7 cut(s) 425, 1359, 1382, 1953, 2528, 2549, 2660
MaeII ACGT 3 cut(s) 246, 1745, 2566
MaeIII GTNAC 4 cut(s) 1664, 1804, 1887, 2167
MalI GATC 8 cut(s) 728, 916, 978, 1251, 1611, 1902, 2555, 2688
MboI GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2553, 2686
MflI RGATCY 2 cut(s) 1900, 2553
MhlI GDGCHC 2 cut(s) 1020, 1545
MlsI TGGCCA 1 cut(s) 105
MluNI TGGCCA 1 cut(s) 105
MlyI GAGTC 5 cut(s) 9, 231, 1389, 2078, 2459
MmeI TCCRAC 4 cut(s) 417, 613, 960, 1731
Mox20I TGGCCA 1 cut(s) 105
MreI CGCCGGCG 1 cut(s) 2749
MroI TCCGGA 1 cut(s) 1861
MroNI GCCGGC 1 cut(s) 2749
MroXI GAANNNNTTC 3 cut(s) 1029, 1481, 2827
MscI TGGCCA 1 cut(s) 105
MseI TTAA 5 cut(s) 1932, 1968, 2144, 2799, 2866
MslI CAYNNNNRTG 2 cut(s) 62, 2064
Msp20I TGGCCA 1 cut(s) 105
MspA1I CMGCKG 1 cut(s) 926
MspI CCGG 9 cut(s) 624, 696, 1272, 1344, 1851, 1862, 2406, 2509, 2750
MspR9I CCNGG 3 cut(s) 190, 697, 1273
MvaI CCWGG 1 cut(s) 190
MwoI GCNNNNNNNGC 7 cut(s) 437, 874, 1086, 1623, 1767, 2088, 2183
NaeI GCCGGC 1 cut(s) 2751
NciI CCSGG 2 cut(s) 697, 1273
NcoI CCATGG 1 cut(s) 1139
NdeII GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2553, 2686
NgoMIV GCCGGC 1 cut(s) 2749
NlaIII CATG 9 cut(s) 162, 186, 207, 829, 1143, 1477, 1805, 2193, 2770
NlaIV GGNNCC 2 cut(s) 164, 844
NmuCI GTSAC 1 cut(s) 1804
NspI RCATGY 1 cut(s) 207
NspV TTCGAA 3 cut(s) 1726, 2129, 2640
PceI AGGCCT 2 cut(s) 1494, 2679
PciSI GCTCTTC 2 cut(s) 117, 2098
PdiI GCCGGC 1 cut(s) 2751
PdmI GAANNNNTTC 3 cut(s) 1029, 1481, 2827
PfoI TCCNGGA 1 cut(s) 695
PleI GAGTC 5 cut(s) 9, 230, 1389, 2077, 2459
PpsI GAGTC 5 cut(s) 9, 230, 1389, 2077, 2459
Psp1406I AACGTT 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 188
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 2 cut(s) 164, 844
PspPI GGNCC 1 cut(s) 407
PsrI GAACNNNNNNTAC 2 cut(s) 1649, 1681
PstI CTGCAG 1 cut(s) 2479
PstNI CAGNNNCTG 6 cut(s) 742, 1083, 1199, 1741, 1897, 2264
PsuI RGATCY 2 cut(s) 1900, 2553
RsaI GTAC 5 cut(s) 25, 150, 818, 1472, 2658
RsaNI GTAC 5 cut(s) 24, 149, 817, 1471, 2657
RseI CAYNNNNRTG 2 cut(s) 62, 2064
SapI GCTCTTC 2 cut(s) 117, 2098
SaqAI TTAA 5 cut(s) 1932, 1968, 2144, 2799, 2866
Sau3AI GATC 8 cut(s) 726, 914, 976, 1249, 1609, 1900, 2553, 2686
Sau96I GGNCC 1 cut(s) 407
ScaI AGTACT 2 cut(s) 25, 2658
SchI GAGTC 5 cut(s) 9, 231, 1389, 2078, 2459
ScrFI CCNGG 3 cut(s) 190, 697, 1273
SduI GDGCHC 2 cut(s) 1020, 1545
SfaNI GCATC 4 cut(s) 952, 2193, 2258, 2694
SfcI CTRYAG 2 cut(s) 1084, 2475
SfuI TTCGAA 3 cut(s) 1726, 2129, 2640
SgrAI CRCCGGYG 1 cut(s) 2749
SinI GGWCC 1 cut(s) 407
SmiMI CAYNNNNRTG 2 cut(s) 62, 2064
SmlI CTYRAG 2 cut(s) 1526, 1660
SmoI CTYRAG 2 cut(s) 1526, 1660
SpeI ACTAGT 1 cut(s) 1952
SseBI AGGCCT 2 cut(s) 1494, 2679
SsiI CCGC 8 cut(s) 680, 924, 1387, 1874, 2107, 2420, 2562, 2753
SspMI CTAG 7 cut(s) 425, 1359, 1382, 1953, 2528, 2549, 2660
StuI AGGCCT 2 cut(s) 1494, 2679
StyD4I CCNGG 3 cut(s) 188, 695, 1271
StyI CCWWGG 5 cut(s) 78, 1139, 1643, 2527, 2674
TaaI ACNGT 7 cut(s) 594, 941, 1085, 1239, 1522, 1887, 1893
TaiI ACGT 3 cut(s) 249, 1748, 2569
TaqI TCGA 9 cut(s) 381, 554, 960, 1061, 1110, 1256, 1726, 2129, 2640
TatI WGTACW 4 cut(s) 23, 148, 1470, 2656
TauI GCSGC 3 cut(s) 683, 1390, 1877
Tru1I TTAA 5 cut(s) 1932, 1968, 2144, 2799, 2866
Tru9I TTAA 5 cut(s) 1932, 1968, 2144, 2799, 2866
TscAI CASTG 6 cut(s) 747, 844, 988, 1200, 1237, 1898
TseFI GTSAC 1 cut(s) 1804
TseI GCWGC 7 cut(s) 677, 868, 1072, 1089, 1603, 2474, 2707
Tsp45I GTSAC 1 cut(s) 1804
TspDTI ATGAA 7 cut(s) 37, 46, 842, 1490, 2178, 2281, 2783
TspGWI ACGGA 1 cut(s) 2271
TspRI CASTG 6 cut(s) 747, 844, 988, 1200, 1237, 1898
VneI GTGCAC 1 cut(s) 1541
VpaK11BI GGWCC 1 cut(s) 407
XapI RAATTY 7 cut(s) 630, 1333, 1422, 1594, 1729, 2038, 2487
XceI RCATGY 1 cut(s) 207
XcmI CCANNNNNNNNNTGG 3 cut(s) 76, 2591, 2773
XmaJI CCTAGG 1 cut(s) 2527
XmiI GTMKAC 1 cut(s) 2385
XmnI GAANNNNTTC 3 cut(s) 1029, 1481, 2827
XspI CTAG 7 cut(s) 425, 1359, 1382, 1953, 2528, 2549, 2660
ZrmI AGTACT 2 cut(s) 25, 2658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.