Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0005520

Physical Location & Seq

Genomic Coordinates Forward (+)

12475115 .. 12488313

Gene Structure 13199 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g14210.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1665 bp

ATGGATTTGACTGAGGGCGTCGGAGAGAGCTCGTCGCCGCCGAGGAGCTTTGTGAGCTTCAATAACTACGATGTGAGGAACGACGTCTATAGCCGATTGGTGGAGAGCGGTCATCCTCTGGCTTTGACTCAACCGGACTTCCGTGACCAGTTGGACGCTCACTTCAATCGCTTGCCTGCTAGTTATGGGCTTGACGTAAACATGGACCGAGTCGAAGATGTTTTGCTGCATCAGAAGCTTCTTGCGTTGGCAAAGGATCCGGAGAAACGGCCTGTTTATCATGTCCGTTTCTTGGAGAGCATCTCTACGGCGGGGACGGATGATGATGAGGATGAACAATTCACTACTAGTGTTTCAACCTCAAGGCTATCATGTAATGAAATCAATGAGGGATCTGCGCAACCACATGATAGAATTAGGAATTGTGCTGTTGACTTTGAACCTTGCTCTAGGCTCGGAGACCTAAATTTGGACGTTAAAAAGAATACTCAGGATGTTGATGAAAGAGATCTGCTGGAGAGATTTCCCAAAAGGCAAGATACATCGCGTATACTCACTCATGAAGTAATTTTTTCATCCATTGACAAGCCTAAGCTTCTTAGCCAGCTTTCTGCGTTACTTTCAGATATAGGACTTAACATCCGTGAAGCACATGTCTTCTCGACCACTGATGGCTACTCCTTGGATGTATTTGTGGTGGATGGATGGCCTGTTGAGGAAACAGATGGTTTAAATGAAGCTATGGAAAAAGCAGTTGCTAGAAGTGAGGGATCGTGGTCAAGATCTTCACATTCCCATTCAGCTGGAGAGAAAATGATTTTGGCACAAGAAAAACCTGGAAGCTGGGAAATAGACAGGAGACTGTTGAAGATAGGAGAAAGAATTGCATCTGGATCCTGTGGAGATTTGCACCGTGGAATTTATCATGGTCAAGATGTTGCTGTTAAGATTCTACGATCTGAGCATTTAAATGCTGCTCTAGAGGATGAGTTTGCTCAAGAAGTGGCCATTCTAAGAGAGGTCCATCACAGAAATGTTGTGCGTTTTATTGGTGCATGCACGAAGTCTCCACATTTATGCATAGTGACAGAGTACATGCCTGGTGGAAGTCTCTATGATTATTTGCACAAGAGTCGTAATGTCTTGAAGCTTTCTGAACTGCTAAAGTTTGCAATTCATGTGTGCAAAGGAATGGAGTATTTGCATCATAATGACATCATCCATAGGGATTTGAAGACAGCAAATCTGCTAATGGACAGTAACAAGGTTGTAAAGGTGGCCGATTTTGGTGTTGCTCGGTTCCAAACACAAGGGGGAGTAATGACTGCAGAGACTGGAACCTATAGATGGATGGCACCTGAGGTTATTAATCATCAACCATATGATCAGAAAGCAGATGTGTTCAGTTTTGCCATTGTACTTTGGGAGCTGGTCACAGCCAAAGTTCCATATGATACAATGACTCCATTACAAGCTGCCTTGGGAGTTAGACAGGGTCTGAGACCTGAACTTCCTACTAACGCACACCCCAAACTGTTAGAATTAATGCAGAGATGTTGGGATGCAGTTCCTTCCAACCGGCCAGCTTTTTCTGACATAGCAGTAGAACTTGAGACCCTCGCACAAGAAGTCCAGGGTAATTCAGAAGCTGCAAATGGCACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ACT Pkinase_Tyr

Protein Analysis

555

Amino Acids

62.13

Weight (kDa)

5.37

Isoelectric Point (pI)

42.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	289 - 493	6.4e-61	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	291 - 488	1.2e-53	Protein kinase domain
ABC1	PF03109	360 - 434	6.1e-06	ABC1 atypical kinase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005520)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g14210 FvH4_2g14210 FvH4_2g14210 FvH4_2g14210 FvH4_2g14210 FvH4_2g14210 FvH4_2g14210
malus_domestica	MD05G1021200.v1.1 MD10G1021900.v1.1
prunus_persica	Prupe.8G024900_v2.0.a1 Prupe.8G024900_v2.0.a1 Prupe.8G024900_v2.0.a1 Prupe.8G024900_v2.0.a1
pyrus_communis	pycom05g01230 pycom10g01520
rosa_chinensis	RchiOBHm_Chr6g0276351
rosa_laevigata	RLG00000013399
rosa_multiflora	Rmu_sc0001919.1_g000011
rosa_roxburghii	Rroxscaffold_7G00192330
rosa_rugosa	Rorug06G0100600 Rorug06G0100700 Rorug06G0100800 Rorug06G0100900 Rorug06G0101000
rosa_samantha	Rh6AG211700 Rh6BG215900 Rh6CG218200 Rh6DG208200
rosa_wichuraiana	Rw6G018480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	87
Acc16I	TGCGCA	1 cut(s)	399
AccB1I	GGYRCC	1 cut(s)	1354
AccBSI	CCGCTC	1 cut(s)	108
AccI	GTMKAC	1 cut(s)	550
AccII	CGCG	1 cut(s)	547
AccIII	TCCGGA	1 cut(s)	259
AciI	CCGC	3 cut(s)	38, 108, 311
AclWI	GGATC	6 cut(s)	251, 264, 400, 778, 888, 901
AcoI	YGGCCR	3 cut(s)	1005, 1278, 1580
AcsI	RAATTY	2 cut(s)	466, 918
AcvI	CACGTG	1 cut(s)	1662
AcyI	GRCGYC	2 cut(s)	18, 84
AfaI	GTAC	2 cut(s)	1094, 1419
AfiI	CCNNNNNNNGG	4 cut(s)	100, 292, 469, 1347
AflIII	ACRYGT	1 cut(s)	652
AgsI	TTSAA	7 cut(s)	61, 166, 357, 440, 868, 1147, 1234
AhlI	ACTAGT	1 cut(s)	347
AjnI	CCWGG	3 cut(s)	835, 1099, 1632
AjuI	GAANNNNNNNTTGG	2 cut(s)	803, 835
Alw21I	GWGCWC	1 cut(s)	32
Alw26I	GTCTC	7 cut(s)	453, 853, 1071, 1115, 1325, 1495, 1607
AlwI	GGATC	6 cut(s)	251, 264, 400, 778, 888, 901
AlwNI	CAGNNNCTG	3 cut(s)	1334, 1498, 1649
Aor13HI	TCCGGA	1 cut(s)	259
AoxI	GGCC	5 cut(s)	269, 707, 1005, 1278, 1580
ApeKI	GCWGC	4 cut(s)	226, 974, 1475, 1649
ApoI	RAATTY	2 cut(s)	466, 918
AseI	ATTAAT	2 cut(s)	1368, 1544
AspLEI	GCGC	1 cut(s)	400
AspS9I	GGNCC	2 cut(s)	205, 1021
AvaII	GGWCC	2 cut(s)	205, 1021
AxyI	CCTNAGG	1 cut(s)	1359
BalI	TGGCCA	1 cut(s)	1007
BamHI	GGATCC	2 cut(s)	256, 893
BanI	GGYRCC	1 cut(s)	1354
BanII	GRGCYC	1 cut(s)	32
BbrPI	CACGTG	1 cut(s)	1662
BbsI	GAAGAC	2 cut(s)	649, 1241
Bbv12I	GWGCWC	1 cut(s)	32
BbvI	GCAGC	4 cut(s)	213, 961, 1462, 1636
BccI	CCATC	7 cut(s)	665, 695, 699, 719, 1032, 1341, 1345
BceAI	ACGGC	2 cut(s)	284, 324
BcgI	CGANNNNNNTGC	2 cut(s)	1115, 1149
BciT130I	CCWGG	3 cut(s)	837, 1101, 1634
BclI	TGATCA	1 cut(s)	1384
BcoDI	GTCTC	7 cut(s)	453, 853, 1071, 1115, 1325, 1495, 1607
BcuI	ACTAGT	1 cut(s)	347
BfaI	CTAG	5 cut(s)	180, 348, 450, 759, 980
BfmI	CTRYAG	3 cut(s)	88, 1326, 1342
BglII	AGATCT	2 cut(s)	508, 782
BisI	GCNGC	5 cut(s)	38, 227, 975, 1476, 1650
BlsI	GCNGC	5 cut(s)	39, 228, 976, 1477, 1651
Bme1390I	CCNGG	3 cut(s)	837, 1101, 1634
Bme18I	GGWCC	2 cut(s)	205, 1021
BmgT120I	GGNCC	2 cut(s)	205, 1021
BmiI	GGNNCC	5 cut(s)	258, 895, 1301, 1339, 1356
BmrFI	CCNGG	3 cut(s)	837, 1101, 1634
BmsI	GCATC	5 cut(s)	238, 309, 896, 1213, 1552
BpiI	GAAGAC	2 cut(s)	649, 1241
BplI	GAGNNNNNCTC	2 cut(s)	287, 319
BpmI	CTGGAG	2 cut(s)	536, 825
Bpu10I	CCTNAGC	1 cut(s)	591
BpuEI	CTTGAG	3 cut(s)	346, 981, 1631
BsaAI	YACGTR	1 cut(s)	1662
BsaHI	GRCGYC	2 cut(s)	18, 84
BsaI	GGTCTC	3 cut(s)	453, 1495, 1607
BsaJI	CCNNGG	5 cut(s)	41, 681, 913, 1479, 1633
BsaWI	WCCGGW	2 cut(s)	133, 259
BsaXI	ACNNNNNCTCC	4 cut(s)	1051, 1081, 1447, 1477
Bsc4I	CCNNNNNNNGG	4 cut(s)	100, 292, 469, 1347
Bse118I	RCCGGY	1 cut(s)	1578
Bse1I	ACTGG	2 cut(s)	148, 1339
Bse21I	CCTNAGG	1 cut(s)	1359
BseAI	TCCGGA	1 cut(s)	259
BseBI	CCWGG	3 cut(s)	837, 1101, 1634
BseDI	CCNNGG	5 cut(s)	41, 681, 913, 1479, 1633
BseLI	CCNNNNNNNGG	4 cut(s)	100, 292, 469, 1347
BseMII	CTCAG	4 cut(s)	503, 951, 1350, 1490
BseNI	ACTGG	2 cut(s)	148, 1339
BseRI	GAGGAG	1 cut(s)	58
BseXI	GCAGC	4 cut(s)	213, 961, 1462, 1636
BseYI	CCCAGC	1 cut(s)	843
Bsh1236I	CGCG	1 cut(s)	547
BshFI	GGCC	5 cut(s)	271, 709, 1007, 1280, 1582
BshNI	GGYRCC	1 cut(s)	1354
BsiHKAI	GWGCWC	1 cut(s)	32
BsiSI	CCGG	3 cut(s)	134, 260, 1579
BslFI	GGGAC	1 cut(s)	328
BslI	CCNNNNNNNGG	4 cut(s)	100, 292, 469, 1347
BsmAI	GTCTC	7 cut(s)	453, 853, 1071, 1115, 1325, 1495, 1607
BsmFI	GGGAC	1 cut(s)	328
BsnI	GGCC	5 cut(s)	271, 709, 1007, 1280, 1582
Bso31I	GGTCTC	3 cut(s)	453, 1495, 1607
Bsp1286I	GDGCHC	1 cut(s)	32
Bsp13I	TCCGGA	1 cut(s)	259
Bsp143I	GATC	8 cut(s)	256, 392, 508, 770, 782, 893, 956, 1384
BspACI	CCGC	3 cut(s)	38, 108, 311
BspANI	GGCC	5 cut(s)	271, 709, 1007, 1280, 1582
BspCNI	CTCAG	5 cut(s)	4, 502, 952, 1351, 1491
BspEI	TCCGGA	1 cut(s)	259
BspFNI	CGCG	1 cut(s)	547
BspHI	TCATGA	1 cut(s)	559
BspLI	GGNNCC	5 cut(s)	258, 895, 1301, 1339, 1356
BspMAI	CTGCAG	1 cut(s)	1330
BspPI	GGATC	6 cut(s)	251, 264, 400, 778, 888, 901
BspT107I	GGYRCC	1 cut(s)	1354
BspTNI	GGTCTC	3 cut(s)	453, 1495, 1607
BsrBI	CCGCTC	1 cut(s)	108
BsrFI	RCCGGY	1 cut(s)	1578
BsrI	ACTGG	2 cut(s)	148, 1339
BssAI	RCCGGY	1 cut(s)	1578
BssECI	CCNNGG	5 cut(s)	41, 681, 913, 1479, 1633
BssMI	GATC	8 cut(s)	256, 392, 508, 770, 782, 893, 956, 1384
BssNAI	GTATAC	1 cut(s)	551
BssNI	GRCGYC	2 cut(s)	18, 84
BssT1I	CCWWGG	2 cut(s)	681, 1479
Bst1107I	GTATAC	1 cut(s)	551
Bst2UI	CCWGG	3 cut(s)	837, 1101, 1634
Bst4CI	ACNGT	4 cut(s)	864, 914, 1259, 1536
BstACI	GRCGYC	2 cut(s)	18, 84
BstBAI	YACGTR	1 cut(s)	1662
BstC8I	GCNNGC	5 cut(s)	173, 177, 605, 1057, 1584
BstDEI	CTNAG	8 cut(s)	12, 489, 591, 599, 960, 1013, 1359, 1499
BstDSI	CCRYGG	1 cut(s)	913
BstFNI	CGCG	1 cut(s)	547
BstHHI	GCGC	1 cut(s)	400
BstKTI	GATC	8 cut(s)	259, 395, 511, 773, 785, 896, 959, 1387
BstMAI	GTCTC	7 cut(s)	453, 853, 1071, 1115, 1325, 1495, 1607
BstMBI	GATC	8 cut(s)	256, 392, 508, 770, 782, 893, 956, 1384
BstMWI	GCNNNNNNNGC	2 cut(s)	54, 235
BstNI	CCWGG	3 cut(s)	837, 1101, 1634
BstNSI	RCATGY	3 cut(s)	656, 1059, 1099
BstSCI	CCNGG	3 cut(s)	835, 1099, 1632
BstSFI	CTRYAG	3 cut(s)	88, 1326, 1342
BstUI	CGCG	1 cut(s)	547
BstV1I	GCAGC	4 cut(s)	213, 961, 1462, 1636
BstV2I	GAAGAC	2 cut(s)	649, 1241
BstX2I	RGATCY	5 cut(s)	256, 392, 508, 782, 893
BstXI	CCANNNNNNTGG	1 cut(s)	803
BstYI	RGATCY	5 cut(s)	256, 392, 508, 782, 893
BstZ17I	GTATAC	1 cut(s)	551
Bsu36I	CCTNAGG	1 cut(s)	1359
BsuRI	GGCC	5 cut(s)	271, 709, 1007, 1280, 1582
BtgI	CCRYGG	1 cut(s)	913
BtgZI	GCGATG	1 cut(s)	528
BtsIMutI	CAGTG	1 cut(s)	666
Cac8I	GCNNGC	5 cut(s)	173, 177, 605, 1057, 1584
CaiI	CAGNNNCTG	3 cut(s)	1334, 1498, 1649
CciI	TCATGA	1 cut(s)	559
CfoI	GCGC	1 cut(s)	400
Cfr10I	RCCGGY	1 cut(s)	1578
Cfr13I	GGNCC	2 cut(s)	205, 1021
CseI	GACGC	2 cut(s)	7, 164
Csp6I	GTAC	2 cut(s)	1093, 1418
CviQI	GTAC	2 cut(s)	1093, 1418
DdeI	CTNAG	8 cut(s)	12, 489, 591, 599, 960, 1013, 1359, 1499
DpnI	GATC	8 cut(s)	258, 394, 510, 772, 784, 895, 958, 1386
DpnII	GATC	8 cut(s)	256, 392, 508, 770, 782, 893, 956, 1384
DraI	TTTAAA	2 cut(s)	732, 969
EaeI	YGGCCR	3 cut(s)	1005, 1278, 1580
Ecl136II	GAGCTC	1 cut(s)	30
Eco130I	CCWWGG	2 cut(s)	681, 1479
Eco24I	GRGCYC	1 cut(s)	32
Eco31I	GGTCTC	3 cut(s)	453, 1495, 1607
Eco47I	GGWCC	2 cut(s)	205, 1021
Eco53kI	GAGCTC	1 cut(s)	30
Eco72I	CACGTG	1 cut(s)	1662
Eco81I	CCTNAGG	1 cut(s)	1359
EcoICRI	GAGCTC	1 cut(s)	30
EcoRII	CCWGG	3 cut(s)	835, 1099, 1632
EcoT14I	CCWWGG	2 cut(s)	681, 1479
EcoT22I	ATGCAT	1 cut(s)	1082
EcoT38I	GRGCYC	1 cut(s)	32
ErhI	CCWWGG	2 cut(s)	681, 1479
FaqI	GGGAC	1 cut(s)	328
FauI	CCCGC	1 cut(s)	304
FauNDI	CATATG	2 cut(s)	1381, 1450
FbaI	TGATCA	1 cut(s)	1384
FblI	GTMKAC	1 cut(s)	550
Fnu4HI	GCNGC	5 cut(s)	38, 227, 975, 1476, 1650
FriOI	GRGCYC	1 cut(s)	32
Fsp4HI	GCNGC	5 cut(s)	38, 227, 975, 1476, 1650
FspBI	CTAG	5 cut(s)	180, 348, 450, 759, 980
FspI	TGCGCA	1 cut(s)	399
GlaI	GCGC	1 cut(s)	399
GluI	GCNGC	5 cut(s)	38, 227, 975, 1476, 1650
GsaI	CCCAGC	1 cut(s)	847
GsuI	CTGGAG	2 cut(s)	536, 825
HaeIII	GGCC	5 cut(s)	271, 709, 1007, 1280, 1582
HapII	CCGG	3 cut(s)	134, 260, 1579
HgaI	GACGC	2 cut(s)	7, 164
HhaI	GCGC	1 cut(s)	400
Hin1I	GRCGYC	2 cut(s)	18, 84
Hin6I	GCGC	1 cut(s)	398
HinP1I	GCGC	1 cut(s)	398
HincII	GTYRAC	1 cut(s)	433
HindII	GTYRAC	1 cut(s)	433
HindIII	AAGCTT	3 cut(s)	236, 593, 1148
HinfI	GANTC	5 cut(s)	127, 210, 949, 1132, 1462
HpaII	CCGG	3 cut(s)	134, 260, 1579
Hpy166II	GTNNAC	3 cut(s)	199, 433, 551
Hpy8I	GTNNAC	3 cut(s)	199, 433, 551
Hpy99I	CGWCG	3 cut(s)	23, 37, 86
HpyAV	CCTTC	1 cut(s)	1581
HpyCH4III	ACNGT	4 cut(s)	864, 914, 1259, 1536
HpyCH4IV	ACGT	4 cut(s)	84, 195, 474, 1661
HpyF10VI	GCNNNNNNNGC	2 cut(s)	54, 235
HpyF3I	CTNAG	8 cut(s)	12, 489, 591, 599, 960, 1013, 1359, 1499
HpySE526I	ACGT	4 cut(s)	84, 195, 474, 1661
Hsp92I	GRCGYC	2 cut(s)	18, 84
HspAI	GCGC	1 cut(s)	398
Kpn2I	TCCGGA	1 cut(s)	259
Ksp22I	TGATCA	1 cut(s)	1384
Kzo9I	GATC	8 cut(s)	256, 392, 508, 770, 782, 893, 956, 1384
LmnI	GCTCC	2 cut(s)	45, 1426
Lsp1109I	GCAGC	4 cut(s)	213, 961, 1462, 1636
LweI	GCATC	5 cut(s)	238, 309, 896, 1213, 1552
MaeI	CTAG	5 cut(s)	180, 348, 450, 759, 980
MaeII	ACGT	4 cut(s)	84, 195, 474, 1661
MaeIII	GTNAC	5 cut(s)	143, 615, 1084, 1259, 1432
MalI	GATC	8 cut(s)	258, 394, 510, 772, 784, 895, 958, 1386
MbiI	CCGCTC	1 cut(s)	108
MboI	GATC	8 cut(s)	256, 392, 508, 770, 782, 893, 956, 1384
MboII	GAAGA	5 cut(s)	227, 649, 777, 880, 1246
MflI	RGATCY	5 cut(s)	256, 392, 508, 782, 893
MhlI	GDGCHC	1 cut(s)	32
MlsI	TGGCCA	1 cut(s)	1007
MluNI	TGGCCA	1 cut(s)	1007
MlyI	GAGTC	4 cut(s)	121, 219, 1141, 1456
MmeI	TCCRAC	2 cut(s)	132, 1599
Mox20I	TGGCCA	1 cut(s)	1007
Mph1103I	ATGCAT	1 cut(s)	1082
MroI	TCCGGA	1 cut(s)	259
MscI	TGGCCA	1 cut(s)	1007
MseI	TTAA	7 cut(s)	477, 636, 731, 945, 968, 1368, 1544
MslI	CAYNNNNRTG	4 cut(s)	969, 1032, 1075, 1209
Msp20I	TGGCCA	1 cut(s)	1007
MspA1I	CMGCKG	1 cut(s)	803
MspI	CCGG	3 cut(s)	134, 260, 1579
MspR9I	CCNGG	3 cut(s)	837, 1101, 1634
MvaI	CCWGG	3 cut(s)	837, 1101, 1634
MvnI	CGCG	1 cut(s)	547
MwoI	GCNNNNNNNGC	2 cut(s)	54, 235
NdeI	CATATG	2 cut(s)	1381, 1450
NdeII	GATC	8 cut(s)	256, 392, 508, 770, 782, 893, 956, 1384
NlaIV	GGNNCC	5 cut(s)	258, 895, 1301, 1339, 1356
NmeAIII	GCCGAG	1 cut(s)	66
NmuCI	GTSAC	3 cut(s)	143, 1084, 1432
NsbI	TGCGCA	1 cut(s)	399
NsiI	ATGCAT	1 cut(s)	1082
NspI	RCATGY	3 cut(s)	656, 1059, 1099
PaeI	GCATGC	1 cut(s)	1059
PagI	TCATGA	1 cut(s)	559
PciI	ACATGT	1 cut(s)	652
PcsI	WCGNNNNNNNCGW	1 cut(s)	38
PfeI	GAWTC	1 cut(s)	949
PflFI	GACNNNGTC	2 cut(s)	209, 1494
PkrI	GCNGC	5 cut(s)	39, 228, 976, 1477, 1651
PleI	GAGTC	4 cut(s)	121, 218, 1140, 1456
PmaCI	CACGTG	1 cut(s)	1662
PmlI	CACGTG	1 cut(s)	1662
PpsI	GAGTC	4 cut(s)	121, 218, 1140, 1456
Ppu21I	YACGTR	1 cut(s)	1662
PscI	ACATGT	1 cut(s)	652
PshBI	ATTAAT	2 cut(s)	1368, 1544
Psp124BI	GAGCTC	1 cut(s)	32
Psp6I	CCWGG	3 cut(s)	835, 1099, 1632
PspCI	CACGTG	1 cut(s)	1662
PspFI	CCCAGC	1 cut(s)	843
PspGI	CCWGG	3 cut(s)	835, 1099, 1632
PspN4I	GGNNCC	5 cut(s)	258, 895, 1301, 1339, 1356
PspPI	GGNCC	2 cut(s)	205, 1021
PstI	CTGCAG	1 cut(s)	1330
PstNI	CAGNNNCTG	3 cut(s)	1334, 1498, 1649
PsuI	RGATCY	5 cut(s)	256, 392, 508, 782, 893
PsyI	GACNNNGTC	2 cut(s)	209, 1494
PvuII	CAGCTG	1 cut(s)	803
RsaI	GTAC	2 cut(s)	1094, 1419
RsaNI	GTAC	2 cut(s)	1093, 1418
RseI	CAYNNNNRTG	4 cut(s)	969, 1032, 1075, 1209
SacI	GAGCTC	1 cut(s)	32
SaqAI	TTAA	7 cut(s)	477, 636, 731, 945, 968, 1368, 1544
SatI	GCNGC	5 cut(s)	38, 227, 975, 1476, 1650
Sau3AI	GATC	8 cut(s)	256, 392, 508, 770, 782, 893, 956, 1384
Sau96I	GGNCC	2 cut(s)	205, 1021
SchI	GAGTC	4 cut(s)	121, 219, 1141, 1456
ScrFI	CCNGG	3 cut(s)	837, 1101, 1634
SduI	GDGCHC	1 cut(s)	32
SfaNI	GCATC	5 cut(s)	238, 309, 896, 1213, 1552
SfcI	CTRYAG	3 cut(s)	88, 1326, 1342
SinI	GGWCC	2 cut(s)	205, 1021
SmiI	ATTTAAAT	1 cut(s)	969
SmiMI	CAYNNNNRTG	4 cut(s)	969, 1032, 1075, 1209
SmlI	CTYRAG	3 cut(s)	361, 996, 1610
SmoI	CTYRAG	3 cut(s)	361, 996, 1610
SpeI	ACTAGT	1 cut(s)	347
SphI	GCATGC	1 cut(s)	1059
SsiI	CCGC	3 cut(s)	38, 108, 311
SspMI	CTAG	5 cut(s)	180, 348, 450, 759, 980
SstI	GAGCTC	1 cut(s)	32
StyD4I	CCNGG	3 cut(s)	835, 1099, 1632
StyI	CCWWGG	2 cut(s)	681, 1479
SwaI	ATTTAAAT	1 cut(s)	969
TaaI	ACNGT	4 cut(s)	864, 914, 1259, 1536
TaiI	ACGT	4 cut(s)	87, 198, 477, 1664
TaqI	TCGA	2 cut(s)	213, 662
TaqII	GACCGA	1 cut(s)	222
TatI	WGTACW	2 cut(s)	1092, 1417
TauI	GCSGC	1 cut(s)	40
TfiI	GAWTC	1 cut(s)	949
Tru1I	TTAA	7 cut(s)	477, 636, 731, 945, 968, 1368, 1544
Tru9I	TTAA	7 cut(s)	477, 636, 731, 945, 968, 1368, 1544
TscAI	CASTG	1 cut(s)	673
TseFI	GTSAC	3 cut(s)	143, 1084, 1432
TseI	GCWGC	4 cut(s)	226, 974, 1475, 1649
Tsp45I	GTSAC	3 cut(s)	143, 1084, 1432
TspDTI	ATGAA	7 cut(s)	348, 393, 516, 564, 576, 750, 1166
TspGWI	ACGGA	4 cut(s)	131, 275, 332, 632
TspRI	CASTG	1 cut(s)	673
Tth111I	GACNNNGTC	2 cut(s)	209, 1494
VpaK11BI	GGWCC	2 cut(s)	205, 1021
VspI	ATTAAT	2 cut(s)	1368, 1544
XapI	RAATTY	2 cut(s)	466, 918
XbaI	TCTAGA	1 cut(s)	979
XceI	RCATGY	3 cut(s)	656, 1059, 1099
XmiI	GTMKAC	1 cut(s)	550
XspI	CTAG	5 cut(s)	180, 348, 450, 759, 980
ZraI	GACGTC	1 cut(s)	85
Zsp2I	ATGCAT	1 cut(s)	1082

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.