Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005520

Physical Location & Seq

Genomic Coordinates Reverse (-)

2708631 .. 2730233

Gene Structure 21603 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1021900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1662 bp

ATGGATTTGACCGAGGGCGTCGGAGAGAGCTCGTCGCCGCCGAGAAGCTTCGTCAGCTTCAGTAACTACGATGTCCGAAACGATGTCTACAACCGCTTGGTGGAGAGCGGCCATGAAGACGCTCTGACTCTGCCCGAATTCCGTGAACAGTTGGACGGTCACTTCAACCGCTTGCCGCCTAGTTACGGACTTGATGTTAACTTGGATAGAGTGGAAGATGTCTTGTTACATCAAAAGCTTCTTGCATTGGCAAAAGACCCTGAGAAGCGGCCCGTGTATCATATCCGTTTCATGGAGAATACTTCTATGGGGACAGACGACGAAGATGATGATGTTCAACAATGTACAAGTGTTGTTTCAACCCCAAGGTCATCATGTGATGAAACTAGTGAAGGAGCTACTCTGTCACATGATAGGAATTGTGCGGCTGACTTTGAACCTTGCTCTAAGCTCGGGGACCTAAATTTGGATGTTAGAAAGAATGCTAAGGATATGGACGGAAGACATCTGACCAAGGATTCTCCCACAAGGCAAGATATACCACATGTTCCCATTCATGAAGTAATATTTTCAACTATTGACAAGCCTAAGCTTCTTAGCCAGCTTTCTTCCTTGCTTTCGGATTTGGAACTTAACATCCGTGAAGCACATGTCTTCTCGACGAGCGATGGCTACTCTTTAGATGTATTTGTGGTAGATGGATGGCCTGTTGAGGATACAGATGGTCTGTATGAAGCTATGGAAAAGGCAATTGCTAGAAGTGAGGGGTCATGGTCAAGATCTTCACATTCTCATTCGGCTGTGGAGAAAGAATTATCGGTGAAAGAAAAGACAGGACTCTGGGAAATAGACCGAAGATTATTAATGATCGGAGACAGAATTGCATCTGGGTCATGTGGAGATTTGTATCGTGGAATTTATCTTGGTCAAGATGTTGCTGTTAAGATTTTGAGATCTGAACATTTGAATGATGCTCTAGAGGATGAGTTTGCTCAAGAAGTGGCAATTTTAAGAGAGGTCCATCATAGTAATGTTGTGCGCTTTATCGGTGCATGTACAAAGTCTCCACATTTGTGCATAGTGACAGAGTATATGCCCGGTGGAAGTCTTTACGATTATTTGCATAAGAATCATAACATCTTGAAGCTTTCAGAACTGCTAAAGTTTGCAATTGATGTCTGTAAAGGAATGGAGTATTTGCATCATAATAACATAATTCATAGGGATCTAAAGACGGCAAATCTGCTAATGGACACTAACAATGTTGTAAAGGTGGCAGATTTTGGTGTTGCTCGGTTTCAGAATCAAGAGGGAGTAATGACAGCAGAGACTGGAACCTATCGATGGATGGCACCTGAGGTTATCAACCATCAACCATATGATCAGAAAGCAGATGTGTTCAGTTTCGCAATTGTACTTTGGGAGCTAGTAACAGCCAAAGTTCCATATGATACCATGACTCCATTGCAAGCTGCCTTGGGAGTTAGACAGGGGCTGAGACCGGAAATTCCGAATAATGGACACCCCAAGGTTTTGGAATTAATGCAGAGTTGCTGGGATTCAGTTCCTTCCAATCGGCCTTCCTTCTCCGATATAACAGCTCAACTTGAATCTCTCCTCCAAGAAGTCCAGGAAATTTCAGAACCCTCCAATGGCACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ACT Pkinase_Tyr

Protein Analysis

554

Amino Acids

62.12

Weight (kDa)

4.96

Isoelectric Point (pI)

49.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACT	PF01842	188 - 233	8.6e-06	ACT domain
PK_Tyr_Ser-Thr	PF07714	290 - 536	5.5e-74	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	292 - 533	1.5e-58	Protein kinase domain
Pkinase_fungal	PF17667	382 - 473	3.5e-06	Fungal protein kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005520)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g14210 FvH4_2g14210 FvH4_2g14210 FvH4_2g14210 FvH4_2g14210 FvH4_2g14210 FvH4_2g14210
malus_domestica	MD05G1021200.v1.1 MD10G1021900.v1.1
prunus_persica	Prupe.8G024900_v2.0.a1 Prupe.8G024900_v2.0.a1 Prupe.8G024900_v2.0.a1 Prupe.8G024900_v2.0.a1
pyrus_communis	pycom05g01230 pycom10g01520
rosa_chinensis	RchiOBHm_Chr6g0276351
rosa_laevigata	RLG00000013399
rosa_multiflora	Rmu_sc0001919.1_g000011
rosa_roxburghii	Rroxscaffold_7G00192330
rosa_rugosa	Rorug06G0100600 Rorug06G0100700 Rorug06G0100800 Rorug06G0100900 Rorug06G0101000
rosa_samantha	Rh6AG211700 Rh6BG215900 Rh6CG218200 Rh6DG208200
rosa_wichuraiana	Rw6G018480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1351
AccBSI	CCGCTC	1 cut(s)	108
AccI	GTMKAC	1 cut(s)	87
AciI	CCGC	7 cut(s)	38, 94, 108, 169, 176, 268, 425
AclWI	GGATC	1 cut(s)	1233
AcoI	YGGCCR	1 cut(s)	109
AcsI	RAATTY	5 cut(s)	137, 463, 915, 1506, 1635
AcuI	CTGAAG	1 cut(s)	43
AcyI	GRCGYC	1 cut(s)	18
AfaI	GTAC	3 cut(s)	346, 1057, 1416
AfiI	CCNNNNNNNGG	7 cut(s)	100, 185, 292, 466, 1344, 1517, 1652
AflIII	ACRYGT	2 cut(s)	544, 649
AgsI	TTSAA	8 cut(s)	166, 338, 360, 437, 573, 967, 1144, 1610
AhlI	ACTAGT	1 cut(s)	386
AjnI	CCWGG	1 cut(s)	1629
AleI	CACNNNNGTG	1 cut(s)	1072
AloI	GAACNNNNNNTCC	2 cut(s)	621, 653
Alw21I	GWGCWC	1 cut(s)	32
Alw26I	GTCTC	4 cut(s)	867, 1068, 1322, 1492
AlwI	GGATC	1 cut(s)	1233
AlwNI	CAGNNNCTG	2 cut(s)	1331, 1495
Ama87I	CYCGRG	1 cut(s)	452
AoxI	GGCC	4 cut(s)	109, 269, 704, 1577
ApeKI	GCWGC	1 cut(s)	1472
ApoI	RAATTY	5 cut(s)	137, 463, 915, 1506, 1635
AseI	ATTAAT	2 cut(s)	863, 1541
AspLEI	GCGC	1 cut(s)	1041
AspS9I	GGNCC	3 cut(s)	270, 457, 1018
AsuC2I	CCSGG	1 cut(s)	1098
AsuHPI	GGTGA	1 cut(s)	832
AvaI	CYCGRG	1 cut(s)	452
AvaII	GGWCC	2 cut(s)	457, 1018
AxyI	CCTNAGG	1 cut(s)	1356
BanI	GGYRCC	1 cut(s)	1351
BanII	GRGCYC	1 cut(s)	32
BbsI	GAAGAC	3 cut(s)	123, 508, 646
Bbv12I	GWGCWC	1 cut(s)	32
BbvI	GCAGC	1 cut(s)	1459
BccI	CCATC	8 cut(s)	662, 692, 696, 716, 1029, 1338, 1342, 1377
BceAI	ACGGC	1 cut(s)	1251
BciT130I	CCWGG	1 cut(s)	1631
BciVI	GTATCC	1 cut(s)	709
BclI	TGATCA	1 cut(s)	1381
BcnI	CCSGG	1 cut(s)	1098
BcoDI	GTCTC	4 cut(s)	867, 1068, 1322, 1492
BcuI	ACTAGT	1 cut(s)	386
BfaI	CTAG	5 cut(s)	180, 387, 756, 977, 1427
BfuI	GTATCC	1 cut(s)	709
BglII	AGATCT	2 cut(s)	779, 953
BisI	GCNGC	6 cut(s)	38, 109, 176, 269, 426, 1473
BlsI	GCNGC	6 cut(s)	39, 110, 177, 270, 427, 1474
Bme1390I	CCNGG	2 cut(s)	1098, 1631
Bme18I	GGWCC	2 cut(s)	457, 1018
BmeT110I	CYCGRG	1 cut(s)	452
BmgT120I	GGNCC	3 cut(s)	270, 457, 1018
BmiI	GGNNCC	3 cut(s)	458, 1336, 1353
BmrFI	CCNGG	2 cut(s)	1098, 1631
BmsI	GCATC	3 cut(s)	893, 961, 1210
BpiI	GAAGAC	3 cut(s)	123, 508, 646
Bpu10I	CCTNAGC	2 cut(s)	486, 588
BpuEI	CTTGAG	1 cut(s)	978
BpuMI	CCSGG	1 cut(s)	1098
Bsa29I	ATCGAT	1 cut(s)	1342
BsaBI	GATNNNNATC	1 cut(s)	906
BsaHI	GRCGYC	1 cut(s)	18
BsaI	GGTCTC	1 cut(s)	1492
BsaJI	CCNNGG	5 cut(s)	12, 365, 513, 1476, 1527
BsaWI	WCCGGW	1 cut(s)	1501
BsaXI	ACNNNNNCTCC	4 cut(s)	1048, 1078, 1444, 1474
Bsc4I	CCNNNNNNNGG	7 cut(s)	100, 185, 292, 466, 1344, 1517, 1652
Bse1I	ACTGG	1 cut(s)	1336
Bse21I	CCTNAGG	1 cut(s)	1356
Bse3DI	GCAATG	1 cut(s)	1463
Bse8I	GATNNNNATC	1 cut(s)	906
BseBI	CCWGG	1 cut(s)	1631
BseCI	ATCGAT	1 cut(s)	1342
BseDI	CCNNGG	5 cut(s)	12, 365, 513, 1476, 1527
BseGI	GGATG	5 cut(s)	475, 636, 707, 988, 1353
BseJI	GATNNNNATC	1 cut(s)	906
BseLI	CCNNNNNNNGG	7 cut(s)	100, 185, 292, 466, 1344, 1517, 1652
BseMI	GCAATG	1 cut(s)	1463
BseMII	CTCAG	3 cut(s)	252, 1347, 1487
BseNI	ACTGG	1 cut(s)	1336
BseRI	GAGGAG	1 cut(s)	1607
BseXI	GCAGC	1 cut(s)	1459
BseYI	CCCAGC	1 cut(s)	1554
BshFI	GGCC	4 cut(s)	111, 271, 706, 1579
BshNI	GGYRCC	1 cut(s)	1351
BshVI	ATCGAT	1 cut(s)	1342
BsiHKAI	GWGCWC	1 cut(s)	32
BsiHKCI	CYCGRG	1 cut(s)	452
BsiSI	CCGG	2 cut(s)	1098, 1502
BslFI	GGGAC	2 cut(s)	325, 470
BslI	CCNNNNNNNGG	7 cut(s)	100, 185, 292, 466, 1344, 1517, 1652
BsmAI	GTCTC	4 cut(s)	867, 1068, 1322, 1492
BsmFI	GGGAC	2 cut(s)	325, 470
BsmI	GAATGC	1 cut(s)	487
BsnI	GGCC	4 cut(s)	111, 271, 706, 1579
Bso31I	GGTCTC	1 cut(s)	1492
BsoBI	CYCGRG	1 cut(s)	452
Bsp1286I	GDGCHC	1 cut(s)	32
Bsp1407I	TGTACA	2 cut(s)	344, 1055
Bsp143I	GATC	5 cut(s)	779, 867, 953, 1225, 1381
BspACI	CCGC	7 cut(s)	38, 94, 108, 169, 176, 268, 425
BspANI	GGCC	4 cut(s)	111, 271, 706, 1579
BspCNI	CTCAG	3 cut(s)	253, 1348, 1488
BspDI	ATCGAT	1 cut(s)	1342
BspHI	TCATGA	1 cut(s)	556
BspLI	GGNNCC	3 cut(s)	458, 1336, 1353
BspPI	GGATC	1 cut(s)	1233
BspT107I	GGYRCC	1 cut(s)	1351
BspTNI	GGTCTC	1 cut(s)	1492
BsrBI	CCGCTC	1 cut(s)	108
BsrDI	GCAATG	1 cut(s)	1463
BsrGI	TGTACA	2 cut(s)	344, 1055
BsrI	ACTGG	1 cut(s)	1336
BssECI	CCNNGG	5 cut(s)	12, 365, 513, 1476, 1527
BssMI	GATC	5 cut(s)	779, 867, 953, 1225, 1381
BssNI	GRCGYC	1 cut(s)	18
BssT1I	CCWWGG	4 cut(s)	365, 513, 1476, 1527
Bst2UI	CCWGG	1 cut(s)	1631
Bst4CI	ACNGT	2 cut(s)	150, 158
BstACI	GRCGYC	1 cut(s)	18
BstAUI	TGTACA	2 cut(s)	344, 1055
BstC8I	GCNNGC	3 cut(s)	173, 602, 1470
BstDEI	CTNAG	7 cut(s)	261, 447, 486, 588, 596, 1356, 1496
BstF5I	GGATG	5 cut(s)	475, 636, 707, 988, 1353
BstHHI	GCGC	1 cut(s)	1041
BstKTI	GATC	5 cut(s)	782, 870, 956, 1228, 1384
BstMAI	GTCTC	4 cut(s)	867, 1068, 1322, 1492
BstMBI	GATC	5 cut(s)	779, 867, 953, 1225, 1381
BstMWI	GCNNNNNNNGC	1 cut(s)	54
BstNI	CCWGG	1 cut(s)	1631
BstNSI	RCATGY	3 cut(s)	548, 653, 1056
BstSCI	CCNGG	2 cut(s)	1096, 1629
BstV1I	GCAGC	1 cut(s)	1459
BstV2I	GAAGAC	3 cut(s)	123, 508, 646
BstX2I	RGATCY	3 cut(s)	779, 953, 1225
BstXI	CCANNNNNNTGG	1 cut(s)	1534
BstYI	RGATCY	3 cut(s)	779, 953, 1225
Bsu15I	ATCGAT	1 cut(s)	1342
Bsu36I	CCTNAGG	1 cut(s)	1356
BsuI	GTATCC	1 cut(s)	709
BsuRI	GGCC	4 cut(s)	111, 271, 706, 1579
BsuTUI	ATCGAT	1 cut(s)	1342
BtgZI	GCGATG	1 cut(s)	681
BtsCI	GGATG	5 cut(s)	475, 636, 707, 988, 1353
Cac8I	GCNNGC	3 cut(s)	173, 602, 1470
CaiI	CAGNNNCTG	2 cut(s)	1331, 1495
CciI	TCATGA	1 cut(s)	556
CfoI	GCGC	1 cut(s)	1041
Cfr13I	GGNCC	3 cut(s)	270, 457, 1018
ClaI	ATCGAT	1 cut(s)	1342
CseI	GACGC	2 cut(s)	7, 128
Csp6I	GTAC	3 cut(s)	345, 1056, 1415
CviQI	GTAC	3 cut(s)	345, 1056, 1415
DdeI	CTNAG	7 cut(s)	261, 447, 486, 588, 596, 1356, 1496
DpnI	GATC	5 cut(s)	781, 869, 955, 1227, 1383
DpnII	GATC	5 cut(s)	779, 867, 953, 1225, 1381
EaeI	YGGCCR	1 cut(s)	109
Ecl136II	GAGCTC	1 cut(s)	30
Eco130I	CCWWGG	4 cut(s)	365, 513, 1476, 1527
Eco24I	GRGCYC	1 cut(s)	32
Eco31I	GGTCTC	1 cut(s)	1492
Eco47I	GGWCC	2 cut(s)	457, 1018
Eco53kI	GAGCTC	1 cut(s)	30
Eco57I	CTGAAG	1 cut(s)	43
Eco81I	CCTNAGG	1 cut(s)	1356
Eco88I	CYCGRG	1 cut(s)	452
EcoICRI	GAGCTC	1 cut(s)	30
EcoO109I	RGGNCCY	1 cut(s)	457
EcoRI	GAATTC	1 cut(s)	137
EcoRII	CCWGG	1 cut(s)	1629
EcoT14I	CCWWGG	4 cut(s)	365, 513, 1476, 1527
EcoT38I	GRGCYC	1 cut(s)	32
ErhI	CCWWGG	4 cut(s)	365, 513, 1476, 1527
FaqI	GGGAC	2 cut(s)	325, 470
FauNDI	CATATG	2 cut(s)	1378, 1447
FbaI	TGATCA	1 cut(s)	1381
FblI	GTMKAC	1 cut(s)	87
Fnu4HI	GCNGC	6 cut(s)	38, 109, 176, 269, 426, 1473
FokI	GGATG	5 cut(s)	482, 623, 714, 995, 1360
FriOI	GRGCYC	1 cut(s)	32
Fsp4HI	GCNGC	6 cut(s)	38, 109, 176, 269, 426, 1473
FspBI	CTAG	5 cut(s)	180, 387, 756, 977, 1427
GlaI	GCGC	1 cut(s)	1040
GluI	GCNGC	6 cut(s)	38, 109, 176, 269, 426, 1473
GsaI	CCCAGC	1 cut(s)	1558
HaeIII	GGCC	4 cut(s)	111, 271, 706, 1579
HapII	CCGG	2 cut(s)	1098, 1502
HgaI	GACGC	2 cut(s)	7, 128
HhaI	GCGC	1 cut(s)	1041
Hin1I	GRCGYC	1 cut(s)	18
Hin6I	GCGC	1 cut(s)	1039
HinP1I	GCGC	1 cut(s)	1039
HincII	GTYRAC	1 cut(s)	199
HindII	GTYRAC	1 cut(s)	199
HindIII	AAGCTT	4 cut(s)	46, 236, 590, 1145
HinfI	GANTC	8 cut(s)	127, 518, 837, 1129, 1303, 1459, 1559, 1610
HpaI	GTTAAC	1 cut(s)	199
HpaII	CCGG	2 cut(s)	1098, 1502
HphI	GGTGA	1 cut(s)	832
Hpy166II	GTNNAC	3 cut(s)	88, 146, 199
Hpy188III	TCNNGA	8 cut(s)	557, 658, 777, 929, 977, 995, 1141, 1307
Hpy8I	GTNNAC	3 cut(s)	88, 146, 199
Hpy99I	CGWCG	4 cut(s)	23, 37, 323, 664
HpyAV	CCTTC	4 cut(s)	386, 1578, 1590, 1594
HpyCH4III	ACNGT	2 cut(s)	150, 158
HpyCH4V	TGCA	9 cut(s)	245, 884, 1052, 1077, 1123, 1169, 1201, 1468, 1546
HpyF10VI	GCNNNNNNNGC	1 cut(s)	54
HpyF3I	CTNAG	7 cut(s)	261, 447, 486, 588, 596, 1356, 1496
Hsp92I	GRCGYC	1 cut(s)	18
HspAI	GCGC	1 cut(s)	1039
Ksp22I	TGATCA	1 cut(s)	1381
KspAI	GTTAAC	1 cut(s)	199
Kzo9I	GATC	5 cut(s)	779, 867, 953, 1225, 1381
LmnI	GCTCC	2 cut(s)	395, 1423
Lsp1109I	GCAGC	1 cut(s)	1459
LweI	GCATC	3 cut(s)	893, 961, 1210
MaeI	CTAG	5 cut(s)	180, 387, 756, 977, 1427
MaeIII	GTNAC	7 cut(s)	62, 158, 182, 225, 405, 1081, 1429
MalI	GATC	5 cut(s)	781, 869, 955, 1227, 1383
MbiI	CCGCTC	1 cut(s)	108
MboI	GATC	5 cut(s)	779, 867, 953, 1225, 1381
MboII	GAAGA	8 cut(s)	128, 227, 335, 513, 600, 646, 774, 867
MfeI	CAATTG	3 cut(s)	750, 1170, 1410
MflI	RGATCY	3 cut(s)	779, 953, 1225
MhlI	GDGCHC	1 cut(s)	32
MlyI	GAGTC	3 cut(s)	121, 831, 1453
MmeI	TCCRAC	1 cut(s)	132
MnlI	CCTC	9 cut(s)	7, 706, 757, 973, 1009, 1303, 1351, 1628, 1657
MseI	TTAA	6 cut(s)	198, 633, 863, 942, 1010, 1541
MslI	CAYNNNNRTG	3 cut(s)	966, 1029, 1072
MspI	CCGG	2 cut(s)	1098, 1502
MspR9I	CCNGG	2 cut(s)	1098, 1631
MunI	CAATTG	3 cut(s)	750, 1170, 1410
Mva1269I	GAATGC	1 cut(s)	487
MvaI	CCWGG	1 cut(s)	1631
MwoI	GCNNNNNNNGC	1 cut(s)	54
NciI	CCSGG	1 cut(s)	1098
NdeI	CATATG	2 cut(s)	1378, 1447
NdeII	GATC	5 cut(s)	779, 867, 953, 1225, 1381
NlaIV	GGNNCC	3 cut(s)	458, 1336, 1353
NmeAIII	GCCGAG	1 cut(s)	66
NmuCI	GTSAC	3 cut(s)	158, 405, 1081
NspI	RCATGY	3 cut(s)	548, 653, 1056
OliI	CACNNNNGTG	1 cut(s)	1072
PagI	TCATGA	1 cut(s)	556
PciI	ACATGT	2 cut(s)	544, 649
PcsI	WCGNNNNNNNCGW	1 cut(s)	38
PctI	GAATGC	1 cut(s)	487
PfeI	GAWTC	5 cut(s)	518, 1129, 1303, 1559, 1610
PfoI	TCCNGGA	1 cut(s)	1629
PkrI	GCNGC	6 cut(s)	39, 110, 177, 270, 427, 1474
PleI	GAGTC	3 cut(s)	121, 831, 1453
PpsI	GAGTC	3 cut(s)	121, 831, 1453
PpuMI	RGGWCCY	1 cut(s)	457
PscI	ACATGT	2 cut(s)	544, 649
PshBI	ATTAAT	2 cut(s)	863, 1541
Psp124BI	GAGCTC	1 cut(s)	32
Psp5II	RGGWCCY	1 cut(s)	457
Psp6I	CCWGG	1 cut(s)	1629
PspFI	CCCAGC	1 cut(s)	1554
PspGI	CCWGG	1 cut(s)	1629
PspN4I	GGNNCC	3 cut(s)	458, 1336, 1353
PspPI	GGNCC	3 cut(s)	270, 457, 1018
PspPPI	RGGWCCY	1 cut(s)	457
PstNI	CAGNNNCTG	2 cut(s)	1331, 1495
PsuI	RGATCY	3 cut(s)	779, 953, 1225
RsaI	GTAC	3 cut(s)	346, 1057, 1416
RsaNI	GTAC	3 cut(s)	345, 1056, 1415
RseI	CAYNNNNRTG	3 cut(s)	966, 1029, 1072
SacI	GAGCTC	1 cut(s)	32
SaqAI	TTAA	6 cut(s)	198, 633, 863, 942, 1010, 1541
SatI	GCNGC	6 cut(s)	38, 109, 176, 269, 426, 1473
Sau3AI	GATC	5 cut(s)	779, 867, 953, 1225, 1381
Sau96I	GGNCC	3 cut(s)	270, 457, 1018
SchI	GAGTC	3 cut(s)	121, 831, 1453
ScrFI	CCNGG	2 cut(s)	1098, 1631
SduI	GDGCHC	1 cut(s)	32
SfaNI	GCATC	3 cut(s)	893, 961, 1210
SinI	GGWCC	2 cut(s)	457, 1018
SmiMI	CAYNNNNRTG	3 cut(s)	966, 1029, 1072
SmlI	CTYRAG	1 cut(s)	993
SmoI	CTYRAG	1 cut(s)	993
SpeI	ACTAGT	1 cut(s)	386
SsiI	CCGC	7 cut(s)	38, 94, 108, 169, 176, 268, 425
SspI	AATATT	1 cut(s)	567
SspMI	CTAG	5 cut(s)	180, 387, 756, 977, 1427
SstI	GAGCTC	1 cut(s)	32
StyD4I	CCNGG	2 cut(s)	1096, 1629
StyI	CCWWGG	4 cut(s)	365, 513, 1476, 1527
TaaI	ACNGT	2 cut(s)	150, 158
TaqI	TCGA	2 cut(s)	659, 1342
TaqII	GACCGA	2 cut(s)	26, 867
TatI	WGTACW	3 cut(s)	344, 1055, 1414
TauI	GCSGC	5 cut(s)	40, 111, 178, 271, 428
TfiI	GAWTC	5 cut(s)	518, 1129, 1303, 1559, 1610
Tru1I	TTAA	6 cut(s)	198, 633, 863, 942, 1010, 1541
Tru9I	TTAA	6 cut(s)	198, 633, 863, 942, 1010, 1541
TseFI	GTSAC	3 cut(s)	158, 405, 1081
TseI	GCWGC	1 cut(s)	1472
Tsp45I	GTSAC	3 cut(s)	158, 405, 1081
TspDTI	ATGAA	7 cut(s)	129, 280, 396, 545, 573, 747, 1208
TspGWI	ACGGA	5 cut(s)	131, 201, 275, 513, 629
VpaK11BI	GGWCC	2 cut(s)	457, 1018
VspI	ATTAAT	2 cut(s)	863, 1541
XapI	RAATTY	5 cut(s)	137, 463, 915, 1506, 1635
XbaI	TCTAGA	1 cut(s)	976
XceI	RCATGY	3 cut(s)	548, 653, 1056
XmiI	GTMKAC	1 cut(s)	87
XspI	CTAG	5 cut(s)	180, 387, 756, 977, 1427

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.