Rorug06G0100900

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005520
Physical Location & Seq
Forward (+)
13414745 .. 13415381
637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0100900.1

Sequence Viewer

Length: 348 bp
ATGGATTCTCAGACCCAGAACACGTCGTTGCAGAGGCTTCAAAATGTGGAGAAGAGAATCGTGAAGGTTTTGGAGCTGGCCGGAAGTGTTATGGACGAGCTTGCAAACCCTACTGGTCCCAGAAGGGAGGCCATCAACAACCATGTTCGCGAGTTCATGCAATTGATCAAGGATATTCAAGTGGCATTGCGGGATGAAATCAAAAGTGCGTGTGATTACCGTCCATTTGAGAAGTGTGATTACAGTTCGAGAATAGCTAACGAGATCTGTTGCAAGAAACTGGAATATGTCATGTCACAGTTGGATGGAATGAAGGAAACAATCGATGATTATCATACTGCGGTTTGA

Protein Analysis

115

Amino Acids

13.28

Weight (kDa)

5.39

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med11 PF10280 8 - 109 6.1e-21 Mediator complex protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 150
AciI CCGC 2 cut(s) 190, 341
AcoI YGGCCR 1 cut(s) 78
AflIII ACRYGT 1 cut(s) 21
AgsI TTSAA 2 cut(s) 41, 179
AjiI CACGTC 1 cut(s) 24
AluBI AGCT 3 cut(s) 76, 100, 257
AluI AGCT 3 cut(s) 76, 100, 257
AoxI GGCC 2 cut(s) 78, 129
AspS9I GGNCC 1 cut(s) 116
AvaII GGWCC 1 cut(s) 116
BarI GAAGNNNNNNTAC 2 cut(s) 224, 256
BccI CCATC 2 cut(s) 140, 299
BclI TGATCA 1 cut(s) 165
BglII AGATCT 1 cut(s) 264
Bme18I GGWCC 1 cut(s) 116
BmgBI CACGTC 1 cut(s) 24
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 118
Bsa29I ATCGAT 1 cut(s) 324
BsaBI GATNNNNATC 1 cut(s) 330
Bse1I ACTGG 2 cut(s) 118, 285
Bse3DI GCAATG 1 cut(s) 185
Bse8I GATNNNNATC 1 cut(s) 330
BseCI ATCGAT 1 cut(s) 324
BseGI GGATG 2 cut(s) 199, 310
BseJI GATNNNNATC 1 cut(s) 330
BseMI GCAATG 1 cut(s) 185
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 2 cut(s) 118, 285
Bsh1236I CGCG 1 cut(s) 150
BshFI GGCC 2 cut(s) 80, 131
BshVI ATCGAT 1 cut(s) 324
BsiSI CCGG 1 cut(s) 81
BslFI GGGAC 1 cut(s) 102
BsmFI GGGAC 1 cut(s) 102
BsnI GGCC 2 cut(s) 80, 131
Bsp143I GATC 2 cut(s) 165, 264
Bsp68I TCGCGA 1 cut(s) 150
BspACI CCGC 2 cut(s) 190, 341
BspANI GGCC 2 cut(s) 80, 131
BspCNI CTCAG 1 cut(s) 22
BspDI ATCGAT 1 cut(s) 324
BspFNI CGCG 1 cut(s) 150
BspLI GGNNCC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 185
BsrI ACTGG 2 cut(s) 118, 285
BssMI GATC 2 cut(s) 165, 264
Bst4CI ACNGT 3 cut(s) 221, 245, 300
Bst6I CTCTTC 1 cut(s) 47
BstC8I GCNNGC 2 cut(s) 78, 102
BstDEI CTNAG 1 cut(s) 9
BstF5I GGATG 2 cut(s) 199, 310
BstFNI CGCG 1 cut(s) 150
BstKTI GATC 2 cut(s) 168, 267
BstMBI GATC 2 cut(s) 165, 264
BstUI CGCG 1 cut(s) 150
BstX2I RGATCY 1 cut(s) 264
BstYI RGATCY 1 cut(s) 264
Bsu15I ATCGAT 1 cut(s) 324
BsuRI GGCC 2 cut(s) 80, 131
BsuTUI ATCGAT 1 cut(s) 324
BtrI CACGTC 1 cut(s) 24
BtsCI GGATG 2 cut(s) 199, 310
BtuMI TCGCGA 1 cut(s) 150
Cac8I GCNNGC 2 cut(s) 78, 102
Cfr13I GGNCC 1 cut(s) 116
ClaI ATCGAT 1 cut(s) 324
CviAII CATG 3 cut(s) 143, 157, 292
CviJI RGCY 6 cut(s) 37, 76, 80, 100, 131, 257
CviKI_1 RGCY 6 cut(s) 37, 76, 80, 100, 131, 257
DdeI CTNAG 1 cut(s) 9
DpnI GATC 2 cut(s) 167, 266
DpnII GATC 2 cut(s) 165, 264
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 116
FaeI CATG 3 cut(s) 146, 160, 295
FaiI YATR 6 cut(s) 92, 144, 158, 288, 293, 336
FaqI GGGAC 1 cut(s) 102
FatI CATG 3 cut(s) 142, 156, 291
FauI CCCGC 1 cut(s) 183
FbaI TGATCA 1 cut(s) 165
FokI GGATG 2 cut(s) 206, 317
HaeIII GGCC 2 cut(s) 80, 131
HapII CCGG 1 cut(s) 81
Hin1II CATG 3 cut(s) 146, 160, 295
HinfI GANTC 2 cut(s) 5, 57
HpaII CCGG 1 cut(s) 81
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 3 cut(s) 61, 149, 249
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 3 cut(s) 58, 117, 307
HpyCH4III ACNGT 3 cut(s) 221, 245, 300
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 4 cut(s) 31, 104, 160, 273
HpyF3I CTNAG 1 cut(s) 9
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 3 cut(s) 146, 160, 295
Ksp22I TGATCA 1 cut(s) 165
Kzo9I GATC 2 cut(s) 165, 264
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 6 cut(s) 29, 62, 94, 99, 133, 266
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 1 cut(s) 294
MalI GATC 2 cut(s) 167, 266
MboI GATC 2 cut(s) 165, 264
MboII GAAGA 1 cut(s) 64
MfeI CAATTG 1 cut(s) 161
MflI RGATCY 1 cut(s) 264
MluCI AATT 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 282
MnlI CCTC 2 cut(s) 27, 121
MspI CCGG 1 cut(s) 81
MunI CAATTG 1 cut(s) 161
MvnI CGCG 1 cut(s) 150
NdeII GATC 2 cut(s) 165, 264
NlaIII CATG 3 cut(s) 146, 160, 295
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 294
NruI TCGCGA 1 cut(s) 150
PfeI GAWTC 2 cut(s) 5, 57
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 1 cut(s) 116
PsuI RGATCY 1 cut(s) 264
RruI TCGCGA 1 cut(s) 150
Sau3AI GATC 2 cut(s) 165, 264
Sau96I GGNCC 1 cut(s) 116
SetI ASST 5 cut(s) 26, 69, 78, 102, 259
SinI GGWCC 1 cut(s) 116
Sse9I AATT 1 cut(s) 161
SsiI CCGC 2 cut(s) 190, 341
TaaI ACNGT 3 cut(s) 221, 245, 300
TaiI ACGT 1 cut(s) 26
TaqI TCGA 2 cut(s) 248, 324
TasI AATT 1 cut(s) 161
TfiI GAWTC 2 cut(s) 5, 57
TseFI GTSAC 1 cut(s) 294
Tsp45I GTSAC 1 cut(s) 294
TspDTI ATGAA 3 cut(s) 145, 210, 326
VpaK11BI GGWCC 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.