Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005520

Physical Location & Seq

Genomic Coordinates Reverse (-)

3577456 .. 3590825

Gene Structure 13370 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1021200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1665 bp

ATGGATTTGACCGAGGGCGTCGGAGAGAGCTCCTCGCCGCCGAGAAGCTTCGTCGGCTTCAGTAACTACGATGTCCGTAACGATGTCTACAACCGCTTGGTGGAGAGCGGCCATGAAGACGCTCTGACTCTGCCGGAGTTCCGTGGACACTTGGACGCGCACTTCAACCGCTTGCCGGCTAGTTATGGACTTGATGTTAACTTGGATAGAGTGGAAGATGTCTTGTTACATCAAAAGCTTCTTGCTTTGGCAAAAGACCCAGAAAAGCGGCCCGTTTATCATGTCCGTTTCATGGAGAATACTTCTACCAGGACAGATGACGACGACGATGATGTTCAACAATTTACAAGTATTGCTTCAACCCAAAGGCCATCATGTGATGAAACTAATGAAGGAGCTGCTCTGTCACATGGAACTAGGAATTGTGCGGTTGACTTTGAACCTTGCTCTAGGCTCGGGGACCTAAATTTGGATGTTAGAAAGAATGCTATGGACATGGACGGAAGACATCTGACGGAGTGTTCTCCCACAAGGCAAGATATACCACATGTTCCCATTCATGAAGTAATATTTTCAACTATTGACAGGCCTAAGCTTCTTAGCCAGCTTTCTTCGTTGCTTTCGGATTTAGGACTTAACATCCGTGAAGCACATGTCTTCTCAACAACTGATGGCTACTCTTTAGATGTATTTGTGGTGGATGGATGGCCTGTTGAGGATGCAGATGGTCTATATGAAGCTATGGAAAAGGCAATTGCGAGAAGTGAGGGGTCGTGGTCAAGATCTTCACATTCTCATTCGGCTGTGGAGAAAGCATTATCGGTGAAACAAAAACTGGGAAACTGGGAAATAGACCGAAGATTATTAAAGATAGGAGACAGAATTGCATCTGGGTCATGTGGAGATTTGTATCGCGGAATTTATCTTGGTCAAGATGTTGCTATTAAGATTTTGAGATCTGAACATTTGAATGATGCCCTAGAGGATGAGTTTGCTCAAGAAGTGGCAATTTTAAGAGAGGTCCATCATAGTAATGTTGTGCGCTTTATCGGTGCATGTACAAAGTCTCCACATTTGTGCATAGTGACAGAGTATATGCCTGGTGGAAGTCTATACGATTATTTGCATAAGAATCATAACATCTTGAAGCTTTCAGAACTGCTAAAGTTTGCGATTGATATCTGTAAAGGAATGGAGTATTTGCATCAGAATAACATAATTCATAGGGATCTGAAGACAGCAAATCTGCTAATGGACACTAACAATGTTGTAAAGGTGGCAGATTTTGGTGTTGCTCGGTTCCAGAATCAAGAGGGTGTAATGACAGCAGAGACTGGAACCTATAGATGGATGGCACCTGAGGTTATCAACCATCAACCGTATGATCAGAAAGCAGATGTGTTCAGTTTTGCAATTGTACTTTGGGAGCTAGTAACAGCCAAAGTTCCATATGATACCTTGACTCCGTTACAAGCTGCCTTGGGAGTTAGACAGGGGCTGAGACCGGAAATTCCCAGTAATGGACACCCTAAGCTTTTGGAATTAATGCAGAGATGTTGGGATTCATTTCCTTCCAATCGGCCTTCCTTCTCCGATATAACAGCTCAACTTGAATCTCTCCTCCAAGAAGTCCAGGAAATTTCGGAACCTTCCAATGGCACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ACT Pkinase_Tyr

Protein Analysis

555

Amino Acids

62.21

Weight (kDa)

5.14

Isoelectric Point (pI)

44.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACT	PF01842	188 - 234	1.6e-07	ACT domain
PK_Tyr_Ser-Thr	PF07714	289 - 537	9.2e-74	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	292 - 534	2.2e-58	Protein kinase domain
Pkinase_fungal	PF17667	383 - 474	9.3e-06	Fungal protein kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005520)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g14210 FvH4_2g14210 FvH4_2g14210 FvH4_2g14210 FvH4_2g14210 FvH4_2g14210 FvH4_2g14210
malus_domestica	MD05G1021200.v1.1 MD10G1021900.v1.1
prunus_persica	Prupe.8G024900_v2.0.a1 Prupe.8G024900_v2.0.a1 Prupe.8G024900_v2.0.a1 Prupe.8G024900_v2.0.a1
pyrus_communis	pycom05g01230 pycom10g01520
rosa_chinensis	RchiOBHm_Chr6g0276351
rosa_laevigata	RLG00000013399
rosa_multiflora	Rmu_sc0001919.1_g000011
rosa_roxburghii	Rroxscaffold_7G00192330
rosa_rugosa	Rorug06G0100600 Rorug06G0100700 Rorug06G0100800 Rorug06G0100900 Rorug06G0101000
rosa_samantha	Rh6AG211700 Rh6BG215900 Rh6CG218200 Rh6DG208200
rosa_wichuraiana	Rw6G018480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1354
AccBSI	CCGCTC	1 cut(s)	108
AccI	GTMKAC	1 cut(s)	87
AccII	CGCG	2 cut(s)	158, 915
AciI	CCGC	7 cut(s)	38, 94, 108, 169, 268, 428, 915
AclWI	GGATC	1 cut(s)	1236
AcoI	YGGCCR	1 cut(s)	109
AcsI	RAATTY	4 cut(s)	466, 918, 1509, 1638
AcuI	CTGAAG	2 cut(s)	43, 1253
AcyI	GRCGYC	1 cut(s)	18
AfaI	GTAC	2 cut(s)	1060, 1419
AfiI	CCNNNNNNNGG	7 cut(s)	100, 175, 292, 469, 1347, 1520, 1655
AflIII	ACRYGT	2 cut(s)	547, 652
AgsI	TTSAA	8 cut(s)	166, 338, 360, 440, 576, 970, 1147, 1613
AjnI	CCWGG	3 cut(s)	308, 1099, 1632
AleI	CACNNNNGTG	1 cut(s)	1075
Alw21I	GWGCWC	1 cut(s)	32
Alw26I	GTCTC	4 cut(s)	870, 1071, 1325, 1495
AlwI	GGATC	1 cut(s)	1236
AlwNI	CAGNNNCTG	2 cut(s)	1334, 1498
Ama87I	CYCGRG	1 cut(s)	455
AoxI	GGCC	6 cut(s)	109, 269, 368, 587, 707, 1580
ApeKI	GCWGC	2 cut(s)	398, 1475
ApoI	RAATTY	4 cut(s)	466, 918, 1509, 1638
AseI	ATTAAT	1 cut(s)	1544
AspLEI	GCGC	2 cut(s)	160, 1044
AspS9I	GGNCC	3 cut(s)	270, 460, 1021
AsuHPI	GGTGA	1 cut(s)	835
AvaI	CYCGRG	1 cut(s)	455
AvaII	GGWCC	2 cut(s)	460, 1021
AxyI	CCTNAGG	1 cut(s)	1359
BanI	GGYRCC	1 cut(s)	1354
BanII	GRGCYC	1 cut(s)	32
BbsI	GAAGAC	4 cut(s)	123, 511, 649, 1241
Bbv12I	GWGCWC	1 cut(s)	32
BbvI	GCAGC	2 cut(s)	385, 1462
BccI	CCATC	9 cut(s)	379, 665, 695, 699, 719, 1032, 1341, 1345, 1380
BciT130I	CCWGG	3 cut(s)	310, 1101, 1634
BclI	TGATCA	1 cut(s)	1384
BcoDI	GTCTC	4 cut(s)	870, 1071, 1325, 1495
BfaI	CTAG	5 cut(s)	180, 417, 450, 980, 1430
BfmI	CTRYAG	1 cut(s)	1342
BglII	AGATCT	2 cut(s)	782, 956
BisI	GCNGC	5 cut(s)	38, 109, 269, 399, 1476
BlsI	GCNGC	5 cut(s)	39, 110, 270, 400, 1477
Bme1390I	CCNGG	3 cut(s)	310, 1101, 1634
Bme18I	GGWCC	2 cut(s)	460, 1021
BmeT110I	CYCGRG	1 cut(s)	455
BmgT120I	GGNCC	3 cut(s)	270, 460, 1021
BmiI	GGNNCC	5 cut(s)	461, 1301, 1339, 1356, 1647
BmrFI	CCNGG	3 cut(s)	310, 1101, 1634
BmrI	ACTGGG	3 cut(s)	845, 853, 1509
BmsI	GCATC	4 cut(s)	709, 896, 964, 1213
BmuI	ACTGGG	3 cut(s)	845, 853, 1509
BpiI	GAAGAC	4 cut(s)	123, 511, 649, 1241
BplI	GAGNNNNNCTC	2 cut(s)	17, 49
Bpu10I	CCTNAGC	2 cut(s)	591, 1530
BpuEI	CTTGAG	1 cut(s)	981
BsaBI	GATNNNNATC	2 cut(s)	909, 1178
BsaHI	GRCGYC	1 cut(s)	18
BsaI	GGTCTC	1 cut(s)	1495
BsaJI	CCNNGG	3 cut(s)	12, 142, 1479
BsaWI	WCCGGW	1 cut(s)	1504
BsaXI	ACNNNNNCTCC	4 cut(s)	1051, 1081, 1447, 1477
Bsc4I	CCNNNNNNNGG	7 cut(s)	100, 175, 292, 469, 1347, 1520, 1655
Bse118I	RCCGGY	1 cut(s)	175
Bse1I	ACTGG	4 cut(s)	840, 848, 1339, 1515
Bse21I	CCTNAGG	1 cut(s)	1359
Bse8I	GATNNNNATC	2 cut(s)	909, 1178
BseBI	CCWGG	3 cut(s)	310, 1101, 1634
BseDI	CCNNGG	3 cut(s)	12, 142, 1479
BseGI	GGATG	7 cut(s)	478, 639, 706, 710, 724, 991, 1356
BseJI	GATNNNNATC	2 cut(s)	909, 1178
BseLI	CCNNNNNNNGG	7 cut(s)	100, 175, 292, 469, 1347, 1520, 1655
BseMII	CTCAG	2 cut(s)	1350, 1490
BseNI	ACTGG	4 cut(s)	840, 848, 1339, 1515
BseRI	GAGGAG	2 cut(s)	22, 1610
BseXI	GCAGC	2 cut(s)	385, 1462
Bsh1236I	CGCG	2 cut(s)	158, 915
BshFI	GGCC	6 cut(s)	111, 271, 370, 589, 709, 1582
BshNI	GGYRCC	1 cut(s)	1354
BsiHKAI	GWGCWC	1 cut(s)	32
BsiHKCI	CYCGRG	1 cut(s)	455
BsiSI	CCGG	3 cut(s)	134, 176, 1505
BslFI	GGGAC	1 cut(s)	473
BslI	CCNNNNNNNGG	7 cut(s)	100, 175, 292, 469, 1347, 1520, 1655
BsmAI	GTCTC	4 cut(s)	870, 1071, 1325, 1495
BsmFI	GGGAC	1 cut(s)	473
BsmI	GAATGC	1 cut(s)	490
BsnI	GGCC	6 cut(s)	111, 271, 370, 589, 709, 1582
Bso31I	GGTCTC	1 cut(s)	1495
BsoBI	CYCGRG	1 cut(s)	455
Bsp1286I	GDGCHC	1 cut(s)	32
Bsp1407I	TGTACA	1 cut(s)	1058
Bsp143I	GATC	4 cut(s)	782, 956, 1228, 1384
BspACI	CCGC	7 cut(s)	38, 94, 108, 169, 268, 428, 915
BspANI	GGCC	6 cut(s)	111, 271, 370, 589, 709, 1582
BspCNI	CTCAG	2 cut(s)	1351, 1491
BspFNI	CGCG	2 cut(s)	158, 915
BspHI	TCATGA	1 cut(s)	559
BspLI	GGNNCC	5 cut(s)	461, 1301, 1339, 1356, 1647
BspPI	GGATC	1 cut(s)	1236
BspT107I	GGYRCC	1 cut(s)	1354
BspTNI	GGTCTC	1 cut(s)	1495
BsrBI	CCGCTC	1 cut(s)	108
BsrFI	RCCGGY	1 cut(s)	175
BsrGI	TGTACA	1 cut(s)	1058
BsrI	ACTGG	4 cut(s)	840, 848, 1339, 1515
BssAI	RCCGGY	1 cut(s)	175
BssECI	CCNNGG	3 cut(s)	12, 142, 1479
BssMI	GATC	4 cut(s)	782, 956, 1228, 1384
BssNI	GRCGYC	1 cut(s)	18
BssT1I	CCWWGG	1 cut(s)	1479
Bst2UI	CCWGG	3 cut(s)	310, 1101, 1634
Bst4CI	ACNGT	1 cut(s)	1380
BstACI	GRCGYC	1 cut(s)	18
BstAUI	TGTACA	1 cut(s)	1058
BstC8I	GCNNGC	3 cut(s)	173, 177, 605
BstDEI	CTNAG	5 cut(s)	591, 599, 1359, 1499, 1530
BstDSI	CCRYGG	1 cut(s)	142
BstF5I	GGATG	7 cut(s)	478, 639, 706, 710, 724, 991, 1356
BstFNI	CGCG	2 cut(s)	158, 915
BstHHI	GCGC	2 cut(s)	160, 1044
BstKTI	GATC	4 cut(s)	785, 959, 1231, 1387
BstMAI	GTCTC	4 cut(s)	870, 1071, 1325, 1495
BstMBI	GATC	4 cut(s)	782, 956, 1228, 1384
BstMWI	GCNNNNNNNGC	1 cut(s)	54
BstNI	CCWGG	3 cut(s)	310, 1101, 1634
BstNSI	RCATGY	3 cut(s)	551, 656, 1059
BstSCI	CCNGG	3 cut(s)	308, 1099, 1632
BstSFI	CTRYAG	1 cut(s)	1342
BstUI	CGCG	2 cut(s)	158, 915
BstV1I	GCAGC	2 cut(s)	385, 1462
BstV2I	GAAGAC	4 cut(s)	123, 511, 649, 1241
BstX2I	RGATCY	3 cut(s)	782, 956, 1228
BstYI	RGATCY	3 cut(s)	782, 956, 1228
Bsu36I	CCTNAGG	1 cut(s)	1359
BsuRI	GGCC	6 cut(s)	111, 271, 370, 589, 709, 1582
BtgI	CCRYGG	1 cut(s)	142
BtsCI	GGATG	7 cut(s)	478, 639, 706, 710, 724, 991, 1356
Cac8I	GCNNGC	3 cut(s)	173, 177, 605
CaiI	CAGNNNCTG	2 cut(s)	1334, 1498
CciI	TCATGA	1 cut(s)	559
CfoI	GCGC	2 cut(s)	160, 1044
Cfr10I	RCCGGY	1 cut(s)	175
Cfr13I	GGNCC	3 cut(s)	270, 460, 1021
CseI	GACGC	3 cut(s)	7, 128, 164
Csp6I	GTAC	2 cut(s)	1059, 1418
CviQI	GTAC	2 cut(s)	1059, 1418
DdeI	CTNAG	5 cut(s)	591, 599, 1359, 1499, 1530
DpnI	GATC	4 cut(s)	784, 958, 1230, 1386
DpnII	GATC	4 cut(s)	782, 956, 1228, 1384
EaeI	YGGCCR	1 cut(s)	109
Ecl136II	GAGCTC	1 cut(s)	30
Eco130I	CCWWGG	1 cut(s)	1479
Eco147I	AGGCCT	1 cut(s)	589
Eco24I	GRGCYC	1 cut(s)	32
Eco31I	GGTCTC	1 cut(s)	1495
Eco32I	GATATC	1 cut(s)	1180
Eco47I	GGWCC	2 cut(s)	460, 1021
Eco53kI	GAGCTC	1 cut(s)	30
Eco57I	CTGAAG	2 cut(s)	43, 1253
Eco81I	CCTNAGG	1 cut(s)	1359
Eco88I	CYCGRG	1 cut(s)	455
EcoICRI	GAGCTC	1 cut(s)	30
EcoO109I	RGGNCCY	1 cut(s)	460
EcoRII	CCWGG	3 cut(s)	308, 1099, 1632
EcoRV	GATATC	1 cut(s)	1180
EcoT14I	CCWWGG	1 cut(s)	1479
EcoT38I	GRGCYC	1 cut(s)	32
ErhI	CCWWGG	1 cut(s)	1479
FalI	AAGNNNNNCTT	2 cut(s)	340, 372
FaqI	GGGAC	1 cut(s)	473
FauNDI	CATATG	1 cut(s)	1450
FbaI	TGATCA	1 cut(s)	1384
FblI	GTMKAC	1 cut(s)	87
Fnu4HI	GCNGC	5 cut(s)	38, 109, 269, 399, 1476
FokI	GGATG	7 cut(s)	485, 626, 713, 717, 731, 998, 1363
FriOI	GRGCYC	1 cut(s)	32
Fsp4HI	GCNGC	5 cut(s)	38, 109, 269, 399, 1476
FspBI	CTAG	5 cut(s)	180, 417, 450, 980, 1430
GlaI	GCGC	2 cut(s)	159, 1043
GluI	GCNGC	5 cut(s)	38, 109, 269, 399, 1476
HaeIII	GGCC	6 cut(s)	111, 271, 370, 589, 709, 1582
HapII	CCGG	3 cut(s)	134, 176, 1505
HgaI	GACGC	3 cut(s)	7, 128, 164
HhaI	GCGC	2 cut(s)	160, 1044
Hin1I	GRCGYC	1 cut(s)	18
Hin6I	GCGC	2 cut(s)	158, 1042
HinP1I	GCGC	2 cut(s)	158, 1042
HincII	GTYRAC	2 cut(s)	199, 433
HindII	GTYRAC	2 cut(s)	199, 433
HindIII	AAGCTT	5 cut(s)	46, 236, 593, 1148, 1532
HinfI	GANTC	6 cut(s)	127, 1132, 1306, 1462, 1562, 1613
HpaI	GTTAAC	1 cut(s)	199
HpaII	CCGG	3 cut(s)	134, 176, 1505
HphI	GGTGA	1 cut(s)	835
Hpy166II	GTNNAC	4 cut(s)	88, 146, 199, 433
Hpy188III	TCNNGA	7 cut(s)	560, 780, 932, 998, 1144, 1303, 1310
Hpy8I	GTNNAC	4 cut(s)	88, 146, 199, 433
Hpy99I	CGWCG	4 cut(s)	23, 56, 326, 329
HpyAV	CCTTC	5 cut(s)	386, 1581, 1593, 1597, 1659
HpyCH4III	ACNGT	1 cut(s)	1380
HpyCH4V	TGCA	8 cut(s)	722, 887, 1055, 1080, 1126, 1204, 1412, 1549
HpyF10VI	GCNNNNNNNGC	1 cut(s)	54
HpyF3I	CTNAG	5 cut(s)	591, 599, 1359, 1499, 1530
Hsp92I	GRCGYC	1 cut(s)	18
HspAI	GCGC	2 cut(s)	158, 1042
KroI	GCCGGC	1 cut(s)	175
KroNI	GCCGGC	1 cut(s)	177
Ksp22I	TGATCA	1 cut(s)	1384
KspAI	GTTAAC	1 cut(s)	199
Kzo9I	GATC	4 cut(s)	782, 956, 1228, 1384
LmnI	GCTCC	3 cut(s)	35, 395, 1426
Lsp1109I	GCAGC	2 cut(s)	385, 1462
LweI	GCATC	4 cut(s)	709, 896, 964, 1213
MaeI	CTAG	5 cut(s)	180, 417, 450, 980, 1430
MaeIII	GTNAC	7 cut(s)	62, 77, 225, 405, 1084, 1432, 1467
MalI	GATC	4 cut(s)	784, 958, 1230, 1386
MbiI	CCGCTC	1 cut(s)	108
MboI	GATC	4 cut(s)	782, 956, 1228, 1384
MboII	GAAGA	8 cut(s)	128, 227, 516, 603, 649, 777, 870, 1246
MfeI	CAATTG	2 cut(s)	753, 1413
MflI	RGATCY	3 cut(s)	782, 956, 1228
MhlI	GDGCHC	1 cut(s)	32
MlyI	GAGTC	2 cut(s)	121, 1456
MnlI	CCTC	9 cut(s)	7, 43, 709, 760, 976, 1012, 1306, 1354, 1631
MroNI	GCCGGC	1 cut(s)	175
MseI	TTAA	6 cut(s)	198, 636, 866, 945, 1013, 1544
MslI	CAYNNNNRTG	3 cut(s)	969, 1032, 1075
MspI	CCGG	3 cut(s)	134, 176, 1505
MspR9I	CCNGG	3 cut(s)	310, 1101, 1634
MunI	CAATTG	2 cut(s)	753, 1413
Mva1269I	GAATGC	1 cut(s)	490
MvaI	CCWGG	3 cut(s)	310, 1101, 1634
MvnI	CGCG	2 cut(s)	158, 915
MwoI	GCNNNNNNNGC	1 cut(s)	54
NaeI	GCCGGC	1 cut(s)	177
NdeI	CATATG	1 cut(s)	1450
NdeII	GATC	4 cut(s)	782, 956, 1228, 1384
NgoMIV	GCCGGC	1 cut(s)	175
NlaIV	GGNNCC	5 cut(s)	461, 1301, 1339, 1356, 1647
NmeAIII	GCCGAG	1 cut(s)	66
NmuCI	GTSAC	2 cut(s)	405, 1084
NspI	RCATGY	3 cut(s)	551, 656, 1059
OliI	CACNNNNGTG	1 cut(s)	1075
PagI	TCATGA	1 cut(s)	559
PceI	AGGCCT	1 cut(s)	589
PciI	ACATGT	2 cut(s)	547, 652
PctI	GAATGC	1 cut(s)	490
PdiI	GCCGGC	1 cut(s)	177
PfeI	GAWTC	4 cut(s)	1132, 1306, 1562, 1613
PfoI	TCCNGGA	1 cut(s)	1632
PkrI	GCNGC	5 cut(s)	39, 110, 270, 400, 1477
PleI	GAGTC	2 cut(s)	121, 1456
PpsI	GAGTC	2 cut(s)	121, 1456
PpuMI	RGGWCCY	1 cut(s)	460
PscI	ACATGT	2 cut(s)	547, 652
PshBI	ATTAAT	1 cut(s)	1544
Psp124BI	GAGCTC	1 cut(s)	32
Psp5II	RGGWCCY	1 cut(s)	460
Psp6I	CCWGG	3 cut(s)	308, 1099, 1632
PspGI	CCWGG	3 cut(s)	308, 1099, 1632
PspN4I	GGNNCC	5 cut(s)	461, 1301, 1339, 1356, 1647
PspPI	GGNCC	3 cut(s)	270, 460, 1021
PspPPI	RGGWCCY	1 cut(s)	460
PstNI	CAGNNNCTG	2 cut(s)	1334, 1498
PsuI	RGATCY	3 cut(s)	782, 956, 1228
RsaI	GTAC	2 cut(s)	1060, 1419
RsaNI	GTAC	2 cut(s)	1059, 1418
RseI	CAYNNNNRTG	3 cut(s)	969, 1032, 1075
SacI	GAGCTC	1 cut(s)	32
SaqAI	TTAA	6 cut(s)	198, 636, 866, 945, 1013, 1544
SatI	GCNGC	5 cut(s)	38, 109, 269, 399, 1476
Sau3AI	GATC	4 cut(s)	782, 956, 1228, 1384
Sau96I	GGNCC	3 cut(s)	270, 460, 1021
SchI	GAGTC	2 cut(s)	121, 1456
ScrFI	CCNGG	3 cut(s)	310, 1101, 1634
SduI	GDGCHC	1 cut(s)	32
SfaNI	GCATC	4 cut(s)	709, 896, 964, 1213
SfcI	CTRYAG	1 cut(s)	1342
SinI	GGWCC	2 cut(s)	460, 1021
SmiMI	CAYNNNNRTG	3 cut(s)	969, 1032, 1075
SmlI	CTYRAG	1 cut(s)	996
SmoI	CTYRAG	1 cut(s)	996
SseBI	AGGCCT	1 cut(s)	589
SsiI	CCGC	7 cut(s)	38, 94, 108, 169, 268, 428, 915
SspI	AATATT	1 cut(s)	570
SspMI	CTAG	5 cut(s)	180, 417, 450, 980, 1430
SstI	GAGCTC	1 cut(s)	32
StuI	AGGCCT	1 cut(s)	589
StyD4I	CCNGG	3 cut(s)	308, 1099, 1632
StyI	CCWWGG	1 cut(s)	1479
TaaI	ACNGT	1 cut(s)	1380
TaqII	GACCGA	2 cut(s)	26, 870
TatI	WGTACW	2 cut(s)	1058, 1417
TauI	GCSGC	3 cut(s)	40, 111, 271
TfiI	GAWTC	4 cut(s)	1132, 1306, 1562, 1613
Tru1I	TTAA	6 cut(s)	198, 636, 866, 945, 1013, 1544
Tru9I	TTAA	6 cut(s)	198, 636, 866, 945, 1013, 1544
TseFI	GTSAC	2 cut(s)	405, 1084
TseI	GCWGC	2 cut(s)	398, 1475
Tsp45I	GTSAC	2 cut(s)	405, 1084
TspDTI	ATGAA	9 cut(s)	129, 280, 396, 405, 548, 576, 750, 1211, 1554
TspGWI	ACGGA	7 cut(s)	65, 131, 275, 516, 530, 632, 1455
VpaK11BI	GGWCC	2 cut(s)	460, 1021
VspI	ATTAAT	1 cut(s)	1544
XapI	RAATTY	4 cut(s)	466, 918, 1509, 1638
XceI	RCATGY	3 cut(s)	551, 656, 1059
XmiI	GTMKAC	1 cut(s)	87
XspI	CTAG	5 cut(s)	180, 417, 450, 980, 1430

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.