FvH4_2g14620

RimP N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015334
Physical Location & Seq
Forward (+)
12835935 .. 12839235
3301 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14620.t1

Sequence Viewer

Length: 924 bp
ATGGAATTAAGCAGAACCTCTGCTATTTCATTTGCATCACTATTCATCACTCCCCGTAGTTTCAGTAGCTCCCACTTCCCTTACCCTTGTCTCCCACTTGACAAGCTTTCTTTCCCATTTCGCTTTGCTCCCAACAAGTCCCTAGCACTGCAGGCTCGCAAGCGAAACTCAGAGCCTGAGCAGCAGCCACTTGTCAGCCCAACTATCATTCAAGAAGTTTCAGAGGATGATGAAGACGACGACGTTATCTTGGATGACTTTGATGATGAGGCATCAATGGATGATGATTTTTACGGGGATGAATCTGCGACGGATAAGGCTGAATTGTATGATGGAGATGGGGGCGGAGGAGGTGGAGTTTCTCTTGCAGGGACATGGTGGGATGAAAAAGCATTTGAAATAGCTGAGGAAGTTATTCTCACCTTTGAGGGTGACTTGAAAATATATGCTTTTAAAACATTGCCAAATTTCACCATTCAAGTGCGCATTGAGAAGCTTTCGAATAAATCTGGCTCCCCAAGTATGGAAGATATAGAAGCTTTCTCTAGAACCTATAGAACAAGACTAGATGAAGCAGAGCTTGCTCGATCAGTACCTGAGAACTTATGTCTTGAGGTGTCATCTCCAGGTGTAGAAAGGGTAGTTCGGATTCCACAAGATTTGGATCGATATAAAGATCGACCTATGTATGTGAAATATGTAACCGAGACGGATGCAACCGGTTCACTATCAGAAAGCGATGGTATTTTCAGGCTTGTTTCTTTCGATATGGAAACAAAATGTTGCATTTGGGGTGTAGCAGACGTGAGGATTAATAGAGAAAAAGCAGGAAAAGGGAGACCACTTAACAAAAAGCAGCGGGAATGGCGATTGAATACACCCTTTGATTCCTTACGGTTAGTCCGGTTGTATTCTGATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF150

Protein Analysis

308

Amino Acids

34.75

Weight (kDa)

4.59

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RimP_N PF02576 153 - 212 2.3e-06 RimP N-terminal domain
DUF7912 PF25498 214 - 304 1.7e-38 Domain of unknown function (DUF7912)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015334)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77122
fragaria_vesca FvH4_2g14620
malus_domestica MD05G1016000.v1.1
prunus_persica Prupe.8G019400_v2.0.a1
pyrus_communis pycom05g00880
rosa_chinensis RchiOBHm_Chr6g0277091
rosa_laevigata RLG00000013334
rosa_roxburghii Rroxscaffold_7G00191620
rosa_rugosa Rorug06G0106300
rosa_samantha Rh6AG216300 Rh6BG220900 Rh6CG213700 Rh6CG213900 Rh6CG223700 Rh6DG213300
rosa_wichuraiana Rw6G018870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 485
AciI CCGC 2 cut(s) 345, 859
AclWI GGATC 1 cut(s) 672
AcsI RAATTY 1 cut(s) 466
AfaI GTAC 1 cut(s) 594
AfiI CCNNNNNNNGG 1 cut(s) 523
AgeI ACCGGT 1 cut(s) 719
AgsI TTSAA 5 cut(s) 212, 398, 439, 479, 874
AjiI CACGTC 1 cut(s) 805
AjnI CCWGG 1 cut(s) 625
AluBI AGCT 6 cut(s) 69, 106, 404, 496, 539, 580
AluI AGCT 6 cut(s) 69, 106, 404, 496, 539, 580
Alw26I GTCTC 3 cut(s) 95, 701, 832
AlwI GGATC 1 cut(s) 672
AlwNI CAGNNNCTG 2 cut(s) 176, 596
ApeKI GCWGC 3 cut(s) 181, 184, 856
ApoI RAATTY 1 cut(s) 466
AseI ATTAAT 1 cut(s) 813
AsiGI ACCGGT 1 cut(s) 719
Asp700I GAANNNNTTC 1 cut(s) 414
AspLEI GCGC 1 cut(s) 486
AsuHPI GGTGA 3 cut(s) 412, 443, 463
AsuII TTCGAA 1 cut(s) 500
BbsI GAAGAC 1 cut(s) 240
BbvCI CCTCAGC 1 cut(s) 405
BbvI GCAGC 3 cut(s) 193, 196, 868
BccI CCATC 3 cut(s) 326, 332, 734
BcgI CGANNNNNNTGC 2 cut(s) 695, 729
BciT130I CCWGG 1 cut(s) 627
BcoDI GTCTC 3 cut(s) 95, 701, 832
BfaI CTAG 3 cut(s) 143, 546, 566
BfmI CTRYAG 2 cut(s) 149, 553
BisI GCNGC 3 cut(s) 182, 185, 857
BlsI GCNGC 3 cut(s) 183, 186, 858
Bme1390I CCNGG 1 cut(s) 627
BmgBI CACGTC 1 cut(s) 805
BmiI GGNNCC 1 cut(s) 514
BmrFI CCNGG 1 cut(s) 627
BmsI GCATC 3 cut(s) 44, 281, 703
BpiI GAAGAC 1 cut(s) 240
BpmI CTGGAG 1 cut(s) 609
Bpu10I CCTNAGC 2 cut(s) 177, 405
Bpu14I TTCGAA 1 cut(s) 500
BpuEI CTTGAG 1 cut(s) 632
Bsa29I ATCGAT 1 cut(s) 667
BsaBI GATNNNNATC 1 cut(s) 663
BsaI GGTCTC 1 cut(s) 832
BsaWI WCCGGW 2 cut(s) 719, 903
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 1 cut(s) 523
Bse118I RCCGGY 1 cut(s) 719
Bse3DI GCAATG 1 cut(s) 458
Bse8I GATNNNNATC 1 cut(s) 663
BseBI CCWGG 1 cut(s) 627
BseCI ATCGAT 1 cut(s) 667
BseGI GGATG 6 cut(s) 232, 259, 286, 304, 388, 718
BseJI GATNNNNATC 1 cut(s) 663
BseLI CCNNNNNNNGG 1 cut(s) 523
BseMI GCAATG 1 cut(s) 458
BseMII CTCAG 4 cut(s) 168, 183, 396, 588
BseRI GAGGAG 1 cut(s) 363
BseXI GCAGC 3 cut(s) 193, 196, 868
BshTI ACCGGT 1 cut(s) 719
BshVI ATCGAT 1 cut(s) 667
BsiSI CCGG 2 cut(s) 720, 904
BslFI GGGAC 2 cut(s) 124, 385
BslI CCNNNNNNNGG 1 cut(s) 523
BsmAI GTCTC 3 cut(s) 95, 701, 832
BsmBI CGTCTC 1 cut(s) 701
BsmFI GGGAC 2 cut(s) 124, 385
Bso31I GGTCTC 1 cut(s) 832
Bsp119I TTCGAA 1 cut(s) 500
Bsp143I GATC 3 cut(s) 587, 664, 676
BspACI CCGC 2 cut(s) 345, 859
BspCNI CTCAG 4 cut(s) 169, 182, 397, 589
BspDI ATCGAT 1 cut(s) 667
BspLI GGNNCC 1 cut(s) 514
BspMAI CTGCAG 1 cut(s) 153
BspPI GGATC 1 cut(s) 672
BspT104I TTCGAA 1 cut(s) 500
BspTNI GGTCTC 1 cut(s) 832
BsrDI GCAATG 1 cut(s) 458
BsrFI RCCGGY 1 cut(s) 719
BssAI RCCGGY 1 cut(s) 719
BssMI GATC 3 cut(s) 587, 664, 676
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 1 cut(s) 897
BstAPI GCANNNNNTGC 1 cut(s) 581
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 4 cut(s) 153, 157, 161, 582
BstDEI CTNAG 4 cut(s) 169, 177, 405, 597
BstF5I GGATG 6 cut(s) 232, 259, 286, 304, 388, 718
BstHHI GCGC 1 cut(s) 486
BstKTI GATC 3 cut(s) 590, 667, 679
BstMAI GTCTC 3 cut(s) 95, 701, 832
BstMBI GATC 3 cut(s) 587, 664, 676
BstMWI GCNNNNNNNGC 4 cut(s) 152, 181, 581, 865
BstNI CCWGG 1 cut(s) 627
BstSCI CCNGG 1 cut(s) 625
BstSFI CTRYAG 2 cut(s) 149, 553
BstV1I GCAGC 3 cut(s) 193, 196, 868
BstV2I GAAGAC 1 cut(s) 240
Bsu15I ATCGAT 1 cut(s) 667
BsuTUI ATCGAT 1 cut(s) 667
BtgZI GCGATG 1 cut(s) 753
BtrI CACGTC 1 cut(s) 805
BtsCI GGATG 6 cut(s) 232, 259, 286, 304, 388, 718
BtsI GCAGTG 1 cut(s) 146
BtsIMutI CAGTG 1 cut(s) 146
Cac8I GCNNGC 4 cut(s) 153, 157, 161, 582
CaiI CAGNNNCTG 2 cut(s) 176, 596
CfoI GCGC 1 cut(s) 486
Cfr10I RCCGGY 1 cut(s) 719
ClaI ATCGAT 1 cut(s) 667
Csp6I GTAC 1 cut(s) 593
CspAI ACCGGT 1 cut(s) 719
CspCI CAANNNNNGTGG 2 cut(s) 642, 677
CviAII CATG 1 cut(s) 375
CviQI GTAC 1 cut(s) 593
DdeI CTNAG 4 cut(s) 169, 177, 405, 597
DpnI GATC 3 cut(s) 589, 666, 678
DpnII GATC 3 cut(s) 587, 664, 676
DraI TTTAAA 1 cut(s) 454
EciI GGCGGA 1 cut(s) 360
Eco31I GGTCTC 1 cut(s) 832
EcoRII CCWGG 1 cut(s) 625
Esp3I CGTCTC 1 cut(s) 701
FaeI CATG 1 cut(s) 378
FalI AAGNNNNNCTT 2 cut(s) 564, 596
FaqI GGGAC 2 cut(s) 124, 385
FatI CATG 1 cut(s) 374
FauI CCCGC 1 cut(s) 852
Fnu4HI GCNGC 3 cut(s) 182, 185, 857
FokI GGATG 6 cut(s) 239, 266, 293, 311, 395, 725
Fsp4HI GCNGC 3 cut(s) 182, 185, 857
FspAI RTGCGCAY 1 cut(s) 485
FspBI CTAG 3 cut(s) 143, 546, 566
FspI TGCGCA 1 cut(s) 485
GlaI GCGC 1 cut(s) 485
GluI GCNGC 3 cut(s) 182, 185, 857
GsuI CTGGAG 1 cut(s) 609
HapII CCGG 2 cut(s) 720, 904
HhaI GCGC 1 cut(s) 486
Hin1II CATG 1 cut(s) 378
Hin6I GCGC 1 cut(s) 484
HinP1I GCGC 1 cut(s) 484
HindIII AAGCTT 3 cut(s) 104, 494, 537
HinfI GANTC 3 cut(s) 302, 649, 887
HpaII CCGG 2 cut(s) 720, 904
HphI GGTGA 3 cut(s) 412, 443, 463
Hpy166II GTNNAC 1 cut(s) 725
Hpy188I TCNGA 5 cut(s) 172, 223, 648, 733, 916
Hpy188III TCNNGA 3 cut(s) 212, 546, 611
Hpy8I GTNNAC 1 cut(s) 725
Hpy99I CGWCG 3 cut(s) 242, 245, 313
HpyCH4III ACNGT 1 cut(s) 897
HpyCH4IV ACGT 2 cut(s) 243, 804
HpyCH4V TGCA 5 cut(s) 35, 151, 368, 716, 786
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 181, 581, 865
HpyF3I CTNAG 4 cut(s) 169, 177, 405, 597
HpySE526I ACGT 2 cut(s) 243, 804
Hsp92II CATG 1 cut(s) 378
HspAI GCGC 1 cut(s) 484
Kzo9I GATC 3 cut(s) 587, 664, 676
LmnI GCTCC 3 cut(s) 74, 133, 518
Lsp1109I GCAGC 3 cut(s) 193, 196, 868
LweI GCATC 3 cut(s) 44, 281, 703
MaeI CTAG 3 cut(s) 143, 546, 566
MaeII ACGT 2 cut(s) 243, 804
MaeIII GTNAC 2 cut(s) 431, 700
MalI GATC 3 cut(s) 589, 666, 678
MboI GATC 3 cut(s) 587, 664, 676
MboII GAAGA 2 cut(s) 245, 539
MluCI AATT 3 cut(s) 5, 323, 466
MnlI CCTC 9 cut(s) 28, 217, 262, 341, 344, 400, 421, 607, 801
MroXI GAANNNNTTC 1 cut(s) 414
MseI TTAA 5 cut(s) 8, 453, 813, 846, 922
MslI CAYNNNNRTG 1 cut(s) 479
MspA1I CMGCKG 1 cut(s) 859
MspI CCGG 2 cut(s) 720, 904
MspR9I CCNGG 1 cut(s) 627
MvaI CCWGG 1 cut(s) 627
MwoI GCNNNNNNNGC 4 cut(s) 152, 181, 581, 865
NdeII GATC 3 cut(s) 587, 664, 676
NlaIII CATG 1 cut(s) 378
NlaIV GGNNCC 1 cut(s) 514
NmuCI GTSAC 1 cut(s) 431
NsbI TGCGCA 1 cut(s) 485
NspV TTCGAA 1 cut(s) 500
PdmI GAANNNNTTC 1 cut(s) 414
PfeI GAWTC 3 cut(s) 302, 649, 887
PinAI ACCGGT 1 cut(s) 719
PkrI GCNGC 3 cut(s) 183, 186, 858
PshBI ATTAAT 1 cut(s) 813
Psp6I CCWGG 1 cut(s) 625
PspGI CCWGG 1 cut(s) 625
PspN4I GGNNCC 1 cut(s) 514
PstI CTGCAG 1 cut(s) 153
PstNI CAGNNNCTG 2 cut(s) 176, 596
RsaI GTAC 1 cut(s) 594
RsaNI GTAC 1 cut(s) 593
RseI CAYNNNNRTG 1 cut(s) 479
SaqAI TTAA 5 cut(s) 8, 453, 813, 846, 922
SatI GCNGC 3 cut(s) 182, 185, 857
Sau3AI GATC 3 cut(s) 587, 664, 676
ScrFI CCNGG 1 cut(s) 627
SfaNI GCATC 3 cut(s) 44, 281, 703
SfcI CTRYAG 2 cut(s) 149, 553
SfuI TTCGAA 1 cut(s) 500
SmiMI CAYNNNNRTG 1 cut(s) 479
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
Sse9I AATT 3 cut(s) 5, 323, 466
SsiI CCGC 2 cut(s) 345, 859
SspMI CTAG 3 cut(s) 143, 546, 566
StyD4I CCNGG 1 cut(s) 625
TaaI ACNGT 1 cut(s) 897
TaiI ACGT 2 cut(s) 246, 807
TaqI TCGA 5 cut(s) 500, 586, 667, 679, 765
TasI AATT 3 cut(s) 5, 323, 466
TfiI GAWTC 3 cut(s) 302, 649, 887
Tru1I TTAA 5 cut(s) 8, 453, 813, 846, 922
Tru9I TTAA 5 cut(s) 8, 453, 813, 846, 922
TscAI CASTG 1 cut(s) 153
TseFI GTSAC 1 cut(s) 431
TseI GCWGC 3 cut(s) 181, 184, 856
Tsp45I GTSAC 1 cut(s) 431
TspDTI ATGAA 6 cut(s) 18, 34, 246, 315, 399, 585
TspGWI ACGGA 2 cut(s) 326, 725
TspRI CASTG 1 cut(s) 153
VspI ATTAAT 1 cut(s) 813
XapI RAATTY 1 cut(s) 466
XbaI TCTAGA 1 cut(s) 545
XmnI GAANNNNTTC 1 cut(s) 414
XspI CTAG 3 cut(s) 143, 546, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.