FvH4_2g17880

Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013250
Physical Location & Seq
Forward (+)
15337038 .. 15341027
3990 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g17880.t1

Sequence Viewer

Length: 1134 bp
ATGTGTGGGAGGGCAAGGTGTACTCTGAGAGCCGACGACATCTCTAGGGCTTGTTATCGGAACCATGGACCCGTCCGTTCTGTTAATATGGACCGGTATCAGCCGAGGTACAATGTGTCGCCGGGAGCGAATTTGCCGGTGGTTCGGAGAGGAGACGGGGCAGATGGTGAAGACGGCGTCGTTCTTCATTGTATGAAATGGGGACTGATTCCGAGTTTTACTAAGAAAACGGAGAAGCCTGATCATTACAGGATGTTCAATGCTCGCTCTGAATCCATTTGTGAGAAGGCGTCTTTTCGTCGTCTGGTTCCTAAGAGCCGCTGCGTGGTTGCAGTAGAAGGGTTTTATGAATGGAAAAAAGATGGTTCTAAGAAGCAGCCTTACTATGTCCATTTTAAGGATGGCCGGCCTCTTCTGTTTGCTGCTCTTTATGATTCATGGGAGAACTCTGAAGGTACTAATGTATATACTGAATGCGAGACTGCAGGTGAGAAACTTTACACCTTCACTATCATCACAACTTCTTCATCTTCAGCTTTGGGTTGGCTCCATGACAGGATGCCAGTTGTTTTGGGTGACAAGGAATCTGTTGATACATGGCTTGATGGTTCTTCGGCTTCCAATTTTGATAAGTTACTTAAACCATATGAAGGGCCTGATCTGGTATGGTACCCGGTAACACCAGCAATGGGTAAGGTGTCTTTTGATGGCCCAGAGTGTAGCAATGAGATAAAATTGAAGACAGATGGCACTAATTCAATCACAAAATTTTTCTCAACAAAGGGAACCAAAAAAGAAGAGATAAACCCAAAGGATACAAGTTTGCATGACTCATCTGTGAAGACTGAGTTTCCAGAAAGCTTAAACGAAGAACCTGAAACCAAAGAGGAGAAGGTGCAACCATCCTCAACTGTGAAATGTGAAGATTCAAAAAGTAGTGTCTCTATATTGTCTCAAGAGGATGCATCTAAGGAGCAGACGAAGCGGGACTATGAAGAGTTTTTAGCTGATTCTAAACCGCTTCCTAATGAATCTGATAAGAAGTCTAGTGCTAGTCCGGCAAAGAAGAAGGTCAACCTGAAGACTTCTCATGACAAGCAACCTACACTATTTTCGTACTTTCGGAAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRAP

Protein Analysis

378

Amino Acids

42.5

Weight (kDa)

7.94

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRAP PF02586 1 - 233 2.3e-69 SOS response associated peptidase (SRAP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26470 AT2G26470
fragaria_vesca FvH4_2g17880 FvH4_2g17880 FvH4_2g17880
malus_domestica MD05G1163100.v1.1
prunus_persica Prupe.8G191400_v2.0.a1
pyrus_communis pycom05g15260
rosa_chinensis RchiOBHm_Chr6g0283131
rosa_laevigata RLG00000012842
rosa_roxburghii Rroxscaffold_7G00185750
rosa_rugosa Rorug06G0151500.1 Rorug06G0151600
rosa_samantha Rh6AG264400 Rh6BG267300 Rh6CG266300 Rh6DG259600
rosa_wichuraiana Rw6G022880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 476
Acc36I ACCTGC 1 cut(s) 476
Acc65I GGTACC 1 cut(s) 669
AccB1I GGYRCC 1 cut(s) 669
AciI CCGC 3 cut(s) 319, 985, 1019
AcoI YGGCCR 1 cut(s) 403
AcsI RAATTY 2 cut(s) 130, 767
AcuI CTGAAG 3 cut(s) 471, 516, 1100
AcyI GRCGYC 2 cut(s) 177, 290
AfaI GTAC 5 cut(s) 22, 110, 457, 671, 1118
AfiI CCNNNNNNNGG 4 cut(s) 325, 397, 650, 689
AgeI ACCGGT 1 cut(s) 93
AgsI TTSAA 4 cut(s) 259, 739, 759, 930
AluBI AGCT 4 cut(s) 536, 861, 1007, 1131
AluI AGCT 4 cut(s) 536, 861, 1007, 1131
Alw26I GTCTC 4 cut(s) 147, 473, 946, 957
AoxI GGCC 4 cut(s) 403, 407, 653, 709
ApeKI GCWGC 3 cut(s) 321, 376, 422
ApoI RAATTY 2 cut(s) 130, 767
AsiGI ACCGGT 1 cut(s) 93
Asp718I GGTACC 1 cut(s) 669
AspS9I GGNCC 4 cut(s) 68, 91, 653, 710
AsuC2I CCSGG 2 cut(s) 123, 674
AsuHPI GGTGA 3 cut(s) 179, 500, 587
AvaII GGWCC 2 cut(s) 68, 91
BaeI ACNNNNGTAYC 2 cut(s) 447, 480
BanI GGYRCC 1 cut(s) 669
BarI GAAGNNNNNNTAC 2 cut(s) 365, 397
BbsI GAAGAC 4 cut(s) 177, 746, 848, 1088
BbvI GCAGC 3 cut(s) 308, 388, 409
BccI CCATC 7 cut(s) 158, 356, 395, 599, 701, 740, 910
BceAI ACGGC 1 cut(s) 190
BciVI GTATCC 1 cut(s) 808
BclI TGATCA 1 cut(s) 241
BcnI CCSGG 2 cut(s) 123, 674
BcoDI GTCTC 4 cut(s) 147, 473, 946, 957
BfaI CTAG 4 cut(s) 45, 1047, 1053, 1132
BfmI CTRYAG 1 cut(s) 483
BfuAI ACCTGC 1 cut(s) 476
BfuI GTATCC 1 cut(s) 808
BisI GCNGC 4 cut(s) 319, 322, 377, 423
BlsI GCNGC 4 cut(s) 320, 323, 378, 424
Bme1390I CCNGG 2 cut(s) 123, 674
Bme18I GGWCC 2 cut(s) 68, 91
BmgT120I GGNCC 4 cut(s) 68, 91, 653, 710
BmiI GGNNCC 6 cut(s) 62, 70, 309, 548, 671, 787
BmrFI CCNGG 2 cut(s) 123, 674
BmsI GCATC 3 cut(s) 549, 952, 974
BpiI GAAGAC 4 cut(s) 177, 746, 848, 1088
BpuEI CTTGAG 1 cut(s) 939
BpuMI CCSGG 2 cut(s) 123, 674
BsaHI GRCGYC 2 cut(s) 177, 290
BsaJI CCNNGG 2 cut(s) 64, 104
BsaWI WCCGGW 1 cut(s) 93
Bsc4I CCNNNNNNNGG 4 cut(s) 325, 397, 650, 689
Bse118I RCCGGY 3 cut(s) 93, 136, 405
Bse1I ACTGG 1 cut(s) 563
Bse3DI GCAATG 2 cut(s) 693, 730
BseDI CCNNGG 2 cut(s) 64, 104
BseGI GGATG 5 cut(s) 258, 406, 564, 902, 967
BseLI CCNNNNNNNGG 4 cut(s) 325, 397, 650, 689
BseMI GCAATG 2 cut(s) 693, 730
BseMII CTCAG 2 cut(s) 17, 837
BseNI ACTGG 1 cut(s) 563
BseRI GAGGAG 2 cut(s) 165, 902
BseXI GCAGC 3 cut(s) 308, 388, 409
BshFI GGCC 4 cut(s) 405, 409, 655, 711
BshNI GGYRCC 1 cut(s) 669
BshTI ACCGGT 1 cut(s) 93
BsiSI CCGG 6 cut(s) 94, 122, 137, 406, 674, 1058
BslFI GGGAC 2 cut(s) 216, 1001
BslI CCNNNNNNNGG 4 cut(s) 325, 397, 650, 689
BsmAI GTCTC 4 cut(s) 147, 473, 946, 957
BsmBI CGTCTC 1 cut(s) 147
BsmFI GGGAC 2 cut(s) 216, 1001
BsmI GAATGC 1 cut(s) 479
BsnI GGCC 4 cut(s) 405, 409, 655, 711
Bsp143I GATC 2 cut(s) 241, 658
Bsp19I CCATGG 1 cut(s) 64
BspACI CCGC 3 cut(s) 319, 985, 1019
BspANI GGCC 4 cut(s) 405, 409, 655, 711
BspCNI CTCAG 2 cut(s) 18, 838
BspHI TCATGA 1 cut(s) 1090
BspLI GGNNCC 6 cut(s) 62, 70, 309, 548, 671, 787
BspMAI CTGCAG 1 cut(s) 487
BspMI ACCTGC 1 cut(s) 476
BspT107I GGYRCC 1 cut(s) 669
BsrDI GCAATG 2 cut(s) 693, 730
BsrFI RCCGGY 3 cut(s) 93, 136, 405
BsrI ACTGG 1 cut(s) 563
BssAI RCCGGY 3 cut(s) 93, 136, 405
BssECI CCNNGG 2 cut(s) 64, 104
BssMI GATC 2 cut(s) 241, 658
BssNI GRCGYC 2 cut(s) 177, 290
BssT1I CCWWGG 1 cut(s) 64
Bst4CI ACNGT 1 cut(s) 913
Bst6I CTCTTC 3 cut(s) 417, 792, 990
BstACI GRCGYC 2 cut(s) 177, 290
BstC8I GCNNGC 2 cut(s) 265, 407
BstDEI CTNAG 6 cut(s) 26, 222, 312, 369, 846, 969
BstDSI CCRYGG 1 cut(s) 64
BstF5I GGATG 5 cut(s) 258, 406, 564, 902, 967
BstKTI GATC 2 cut(s) 244, 661
BstMAI GTCTC 4 cut(s) 147, 473, 946, 957
BstMBI GATC 2 cut(s) 241, 658
BstMWI GCNNNNNNNGC 2 cut(s) 982, 1058
BstSCI CCNGG 2 cut(s) 121, 672
BstSFI CTRYAG 1 cut(s) 483
BstV1I GCAGC 3 cut(s) 308, 388, 409
BstV2I GAAGAC 4 cut(s) 177, 746, 848, 1088
BsuI GTATCC 1 cut(s) 808
BsuRI GGCC 4 cut(s) 405, 409, 655, 711
BtgI CCRYGG 1 cut(s) 64
BtsCI GGATG 5 cut(s) 258, 406, 564, 902, 967
BveI ACCTGC 1 cut(s) 476
Cac8I GCNNGC 2 cut(s) 265, 407
CciI TCATGA 1 cut(s) 1090
Cfr10I RCCGGY 3 cut(s) 93, 136, 405
Cfr13I GGNCC 4 cut(s) 68, 91, 653, 710
CseI GACGC 2 cut(s) 166, 279
Csp6I GTAC 5 cut(s) 21, 109, 456, 670, 1117
CspAI ACCGGT 1 cut(s) 93
CviAII CATG 6 cut(s) 65, 438, 551, 597, 827, 1091
CviQI GTAC 5 cut(s) 21, 109, 456, 670, 1117
DdeI CTNAG 6 cut(s) 26, 222, 312, 369, 846, 969
DpnI GATC 2 cut(s) 243, 660
DpnII GATC 2 cut(s) 241, 658
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 3 cut(s) 417, 792, 990
EarI CTCTTC 3 cut(s) 417, 792, 990
Eco130I CCWWGG 1 cut(s) 64
Eco47I GGWCC 2 cut(s) 68, 91
Eco57I CTGAAG 3 cut(s) 471, 516, 1100
EcoO109I RGGNCCY 1 cut(s) 653
EcoT14I CCWWGG 1 cut(s) 64
EcoT22I ATGCAT 1 cut(s) 967
ErhI CCWWGG 1 cut(s) 64
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 6 cut(s) 68, 441, 554, 600, 830, 1094
FaqI GGGAC 2 cut(s) 216, 1001
FatI CATG 6 cut(s) 64, 437, 550, 596, 826, 1090
FauI CCCGC 1 cut(s) 978
FauNDI CATATG 1 cut(s) 646
FbaI TGATCA 1 cut(s) 241
Fnu4HI GCNGC 4 cut(s) 319, 322, 377, 423
FokI GGATG 5 cut(s) 265, 413, 571, 889, 974
FseI GGCCGGCC 1 cut(s) 409
Fsp4HI GCNGC 4 cut(s) 319, 322, 377, 423
FspBI CTAG 4 cut(s) 45, 1047, 1053, 1132
GluI GCNGC 4 cut(s) 319, 322, 377, 423
HaeIII GGCC 4 cut(s) 405, 409, 655, 711
HapII CCGG 6 cut(s) 94, 122, 137, 406, 674, 1058
HgaI GACGC 2 cut(s) 166, 279
Hin1I GRCGYC 2 cut(s) 177, 290
Hin1II CATG 6 cut(s) 68, 441, 554, 600, 830, 1094
HincII GTYRAC 1 cut(s) 1075
HindII GTYRAC 1 cut(s) 1075
HindIII AAGCTT 1 cut(s) 859
HinfI GANTC 8 cut(s) 208, 272, 434, 584, 830, 926, 1010, 1031
HpaII CCGG 6 cut(s) 94, 122, 137, 406, 674, 1058
HphI GGTGA 3 cut(s) 179, 500, 587
Hpy166II GTNNAC 2 cut(s) 21, 1075
Hpy188I TCNGA 8 cut(s) 27, 60, 147, 213, 271, 451, 1036, 1125
Hpy188III TCNNGA 3 cut(s) 854, 956, 1091
Hpy8I GTNNAC 2 cut(s) 21, 1075
Hpy99I CGWCG 3 cut(s) 38, 182, 303
HpyAV CCTTC 7 cut(s) 280, 332, 446, 514, 644, 886, 1063
HpyCH4III ACNGT 1 cut(s) 913
HpyCH4V TGCA 5 cut(s) 332, 485, 826, 898, 965
HpyF10VI GCNNNNNNNGC 2 cut(s) 982, 1058
HpyF3I CTNAG 6 cut(s) 26, 222, 312, 369, 846, 969
Hsp92I GRCGYC 2 cut(s) 177, 290
Hsp92II CATG 6 cut(s) 68, 441, 554, 600, 830, 1094
KpnI GGTACC 1 cut(s) 673
KroI GCCGGC 1 cut(s) 405
KroNI GCCGGC 1 cut(s) 407
Ksp22I TGATCA 1 cut(s) 241
Kzo9I GATC 2 cut(s) 241, 658
LmnI GCTCC 3 cut(s) 125, 552, 973
Lsp1109I GCAGC 3 cut(s) 308, 388, 409
LweI GCATC 3 cut(s) 549, 952, 974
MaeI CTAG 4 cut(s) 45, 1047, 1053, 1132
MaeIII GTNAC 3 cut(s) 575, 633, 676
MalI GATC 2 cut(s) 243, 660
MboI GATC 2 cut(s) 241, 658
MluCI AATT 5 cut(s) 130, 622, 734, 754, 767
MlyI GAGTC 1 cut(s) 824
MnlI CCTC 7 cut(s) 3, 99, 143, 420, 880, 916, 952
Mph1103I ATGCAT 1 cut(s) 967
MroNI GCCGGC 1 cut(s) 405
MseI TTAA 4 cut(s) 84, 396, 639, 863
MspA1I CMGCKG 1 cut(s) 321
MspI CCGG 6 cut(s) 94, 122, 137, 406, 674, 1058
MspR9I CCNGG 2 cut(s) 123, 674
Mva1269I GAATGC 1 cut(s) 479
MwoI GCNNNNNNNGC 2 cut(s) 982, 1058
NaeI GCCGGC 1 cut(s) 407
NciI CCSGG 2 cut(s) 123, 674
NcoI CCATGG 1 cut(s) 64
NdeI CATATG 1 cut(s) 646
NdeII GATC 2 cut(s) 241, 658
NgoMIV GCCGGC 1 cut(s) 405
NlaIII CATG 6 cut(s) 68, 441, 554, 600, 830, 1094
NlaIV GGNNCC 6 cut(s) 62, 70, 309, 548, 671, 787
NmeAIII GCCGAG 1 cut(s) 129
NmuCI GTSAC 1 cut(s) 575
NsiI ATGCAT 1 cut(s) 967
PagI TCATGA 1 cut(s) 1090
PaqCI CACCTGC 1 cut(s) 476
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PctI GAATGC 1 cut(s) 479
PdiI GCCGGC 1 cut(s) 407
PfeI GAWTC 7 cut(s) 208, 272, 434, 584, 926, 1010, 1031
PflFI GACNNNGTC 1 cut(s) 176
PinAI ACCGGT 1 cut(s) 93
PkrI GCNGC 4 cut(s) 320, 323, 378, 424
PleI GAGTC 1 cut(s) 824
PpsI GAGTC 1 cut(s) 824
PspN4I GGNNCC 6 cut(s) 62, 70, 309, 548, 671, 787
PspPI GGNCC 4 cut(s) 68, 91, 653, 710
PstI CTGCAG 1 cut(s) 487
PsyI GACNNNGTC 1 cut(s) 176
RigI GGCCGGCC 1 cut(s) 409
RsaI GTAC 5 cut(s) 22, 110, 457, 671, 1118
RsaNI GTAC 5 cut(s) 21, 109, 456, 670, 1117
SaqAI TTAA 4 cut(s) 84, 396, 639, 863
SatI GCNGC 4 cut(s) 319, 322, 377, 423
Sau3AI GATC 2 cut(s) 241, 658
Sau96I GGNCC 4 cut(s) 68, 91, 653, 710
SchI GAGTC 1 cut(s) 824
ScrFI CCNGG 2 cut(s) 123, 674
SfaNI GCATC 3 cut(s) 549, 952, 974
SfcI CTRYAG 1 cut(s) 483
SinI GGWCC 2 cut(s) 68, 91
SmlI CTYRAG 1 cut(s) 954
SmoI CTYRAG 1 cut(s) 954
Sse9I AATT 5 cut(s) 130, 622, 734, 754, 767
SsiI CCGC 3 cut(s) 319, 985, 1019
SspMI CTAG 4 cut(s) 45, 1047, 1053, 1132
StyD4I CCNGG 2 cut(s) 121, 672
StyI CCWWGG 1 cut(s) 64
TaaI ACNGT 1 cut(s) 913
TasI AATT 5 cut(s) 130, 622, 734, 754, 767
TatI WGTACW 1 cut(s) 20
TauI GCSGC 1 cut(s) 321
TfiI GAWTC 7 cut(s) 208, 272, 434, 584, 926, 1010, 1031
Tru1I TTAA 4 cut(s) 84, 396, 639, 863
Tru9I TTAA 4 cut(s) 84, 396, 639, 863
TseFI GTSAC 1 cut(s) 575
TseI GCWGC 3 cut(s) 321, 376, 422
Tsp45I GTSAC 1 cut(s) 575
TspDTI ATGAA 8 cut(s) 176, 209, 363, 426, 516, 663, 1008, 1044
TspGWI ACGGA 2 cut(s) 65, 245
Tth111I GACNNNGTC 1 cut(s) 176
VpaK11BI GGWCC 2 cut(s) 68, 91
XapI RAATTY 2 cut(s) 130, 767
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XspI CTAG 4 cut(s) 45, 1047, 1053, 1132
Zsp2I ATGCAT 1 cut(s) 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.