MD05G1163100.v1.1

Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013250
Physical Location & Seq
Forward (+)
29203344 .. 29206663
3320 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1163100.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGTGTGGAAGGGCTCGCTGTACTCTCAGAGCCGATGACGTCACTAGGGCTTGCCACCGCACCCACGCCCCCGTCCGCACCGTTAACATGGACCGGTATCGTCCGTCGTACAATGTGTCGCCTGGATCGAATTTGCCTGTTGTTCGGAGAGAAGACGGGGCCGATGGCGACGGCGTCGTTCTTCAATGTATGAAATGGGGGCTAATTCCTAGTTTCACCAAGAAAACCGAAAAGCCCGATTTCTACAGGATGTTTAATGCTCGGTCTGAGTCCATTTGTGAAAAGGCTTCTTTTCGTCGTTTGGTTCCTAAAAGCCGCTGCATTGTTGCGGTTGAAGGTTTTTATGAATGGAAAAAAGATGGTTCTAAAAAGCAGCCTTACTATGTCCATTTCAAGGACGGCAGGCCTCTTCTGTTTGCTGCTCTTTATGATTCTTGGGAGAACTCTGAAGGAGAGAAACTTTACACATTCACTATCATAACAACTTCTTCATCATCAGCTTTAGGGTGGCTGCATGACAGGATGCCTGTTATCTTGGGTGATAAAGAATCAACTGACACATGGCTAGATGGTTCTTCATCATCCAATTTTGATTCATTACTTAAACCATTTGAAGGTCCAGATTTGGTATGGTACCCGGTGACATCGGCAATGGGTAAGGTGTCTTTTGATGGGCCAGAGTGCATCAATGAGATACAGTTGAAGACCGAGAGGAATAATTCCATCACAAAGTTTTTTTCGGCGAAGGGAACCAAAAAAGAAGAATTAAACCCAAAGGACACAAGTTCTGATGACACGTCTGCAAAGAAAGATTTGTCAAAAATGGTGAAAGAAGAACCTGAAAGCAAAGAGGACACAGAACAGCCGTACTCTACTCAACAATGTGAAGATGAATCAAAATGTAATGTGTCTACATTGTCTCAAGAAGGTGTATCTAAGGGCCAAGCAAAGCGAGACTATGAAGAATTTTCAGCTGATTCTAAGCCAGTTGCTTATGTAACGAATAAGAAATCTGCTACGAATAAGAAATCTGCTAGTCTGGCGAAGAAGAAAGTTAACCCGAAGAGTTCTCTAGACAAGCAACCTACCCTATTTTCGTACTTCGGTAAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRAP

Protein Analysis

372

Amino Acids

41.77

Weight (kDa)

8.75

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRAP PF02586 1 - 221 9.4e-72 SOS response associated peptidase (SRAP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26470 AT2G26470
fragaria_vesca FvH4_2g17880 FvH4_2g17880 FvH4_2g17880
malus_domestica MD05G1163100.v1.1
prunus_persica Prupe.8G191400_v2.0.a1
pyrus_communis pycom05g15260
rosa_chinensis RchiOBHm_Chr6g0283131
rosa_laevigata RLG00000012842
rosa_roxburghii Rroxscaffold_7G00185750
rosa_rugosa Rorug06G0151500.1 Rorug06G0151600
rosa_samantha Rh6AG264400 Rh6BG267300 Rh6CG266300 Rh6DG259600
rosa_wichuraiana Rw6G022880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 42
Acc65I GGTACC 1 cut(s) 633
AccB1I GGYRCC 1 cut(s) 633
AccI GTMKAC 1 cut(s) 911
AciI CCGC 4 cut(s) 58, 76, 316, 329
AclWI GGATC 1 cut(s) 133
AcsI RAATTY 2 cut(s) 130, 965
AcuI CTGAAG 1 cut(s) 468
AcyI GRCGYC 2 cut(s) 39, 174
AfaI GTAC 5 cut(s) 22, 110, 635, 869, 1100
AfiI CCNNNNNNNGG 2 cut(s) 394, 614
AflIII ACRYGT 1 cut(s) 795
AgeI ACCGGT 1 cut(s) 93
AgsI TTSAA 5 cut(s) 185, 335, 394, 614, 703
AjiI CACGTC 1 cut(s) 798
AjnI CCWGG 1 cut(s) 121
AluBI AGCT 3 cut(s) 500, 974, 1113
AluI AGCT 3 cut(s) 500, 974, 1113
Alw26I GTCTC 2 cut(s) 924, 948
AlwI GGATC 1 cut(s) 133
AoxI GGCC 4 cut(s) 159, 404, 674, 940
ApeKI GCWGC 4 cut(s) 318, 373, 419, 511
ApoI RAATTY 2 cut(s) 130, 965
AsiGI ACCGGT 1 cut(s) 93
Asp718I GGTACC 1 cut(s) 633
AspS9I GGNCC 5 cut(s) 91, 159, 617, 674, 940
AsuC2I CCSGG 1 cut(s) 638
AsuHPI GGTGA 4 cut(s) 208, 551, 652, 838
AvaII GGWCC 2 cut(s) 91, 617
BanI GGYRCC 1 cut(s) 633
BanII GRGCYC 1 cut(s) 16
BbsI GAAGAC 2 cut(s) 159, 710
BbvI GCAGC 4 cut(s) 305, 385, 406, 498
BccI CCATC 5 cut(s) 158, 353, 563, 665, 731
BceAI ACGGC 3 cut(s) 187, 415, 850
BciT130I CCWGG 1 cut(s) 123
BcnI CCSGG 1 cut(s) 638
BcoDI GTCTC 2 cut(s) 924, 948
BfaI CTAG 6 cut(s) 45, 210, 566, 1035, 1073, 1114
BfmI CTRYAG 1 cut(s) 244
BisI GCNGC 5 cut(s) 316, 319, 374, 420, 512
BlsI GCNGC 5 cut(s) 317, 320, 375, 421, 513
Bme1390I CCNGG 2 cut(s) 123, 638
Bme18I GGWCC 2 cut(s) 91, 617
BmgBI CACGTC 1 cut(s) 798
BmgT120I GGNCC 5 cut(s) 91, 159, 617, 674, 940
BmiI GGNNCC 4 cut(s) 160, 306, 635, 751
BmrFI CCNGG 2 cut(s) 123, 638
BmsI GCATC 2 cut(s) 513, 693
BpiI GAAGAC 2 cut(s) 159, 710
BpuEI CTTGAG 1 cut(s) 906
BpuMI CCSGG 1 cut(s) 638
BsaHI GRCGYC 2 cut(s) 39, 174
BsaWI WCCGGW 1 cut(s) 93
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 614
Bse118I RCCGGY 1 cut(s) 93
Bse1I ACTGG 1 cut(s) 986
Bse3DI GCAATG 1 cut(s) 657
BseBI CCWGG 1 cut(s) 123
BseGI GGATG 3 cut(s) 255, 528, 581
BseLI CCNNNNNNNGG 2 cut(s) 394, 614
BseMI GCAATG 1 cut(s) 657
BseMII CTCAG 2 cut(s) 40, 258
BseNI ACTGG 1 cut(s) 986
BseXI GCAGC 4 cut(s) 305, 385, 406, 498
BshFI GGCC 4 cut(s) 161, 406, 676, 942
BshNI GGYRCC 1 cut(s) 633
BshTI ACCGGT 1 cut(s) 93
BsiSI CCGG 2 cut(s) 94, 638
BslI CCNNNNNNNGG 2 cut(s) 394, 614
BsmAI GTCTC 2 cut(s) 924, 948
BsnI GGCC 4 cut(s) 161, 406, 676, 942
Bsp1286I GDGCHC 1 cut(s) 16
Bsp143I GATC 1 cut(s) 125
BspACI CCGC 4 cut(s) 58, 76, 316, 329
BspANI GGCC 4 cut(s) 161, 406, 676, 942
BspCNI CTCAG 2 cut(s) 39, 259
BspLI GGNNCC 4 cut(s) 160, 306, 635, 751
BspPI GGATC 1 cut(s) 133
BspT107I GGYRCC 1 cut(s) 633
BsrDI GCAATG 1 cut(s) 657
BsrFI RCCGGY 1 cut(s) 93
BsrI ACTGG 1 cut(s) 986
BssAI RCCGGY 1 cut(s) 93
BssMI GATC 1 cut(s) 125
BssNI GRCGYC 2 cut(s) 39, 174
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 2 cut(s) 82, 699
Bst6I CTCTTC 2 cut(s) 414, 1058
BstACI GRCGYC 2 cut(s) 39, 174
BstC8I GCNNGC 3 cut(s) 16, 52, 404
BstDEI CTNAG 4 cut(s) 26, 267, 936, 981
BstF5I GGATG 3 cut(s) 255, 528, 581
BstKTI GATC 1 cut(s) 128
BstMAI GTCTC 2 cut(s) 924, 948
BstMBI GATC 1 cut(s) 125
BstMWI GCNNNNNNNGC 1 cut(s) 1040
BstNI CCWGG 1 cut(s) 123
BstSCI CCNGG 2 cut(s) 121, 636
BstSFI CTRYAG 1 cut(s) 244
BstV1I GCAGC 4 cut(s) 305, 385, 406, 498
BstV2I GAAGAC 2 cut(s) 159, 710
BsuRI GGCC 4 cut(s) 161, 406, 676, 942
BtrI CACGTC 1 cut(s) 798
BtsCI GGATG 3 cut(s) 255, 528, 581
Cac8I GCNNGC 3 cut(s) 16, 52, 404
Cfr10I RCCGGY 1 cut(s) 93
Cfr13I GGNCC 5 cut(s) 91, 159, 617, 674, 940
CseI GACGC 1 cut(s) 163
Csp6I GTAC 5 cut(s) 21, 109, 634, 868, 1099
CspAI ACCGGT 1 cut(s) 93
CviAII CATG 3 cut(s) 88, 515, 561
CviQI GTAC 5 cut(s) 21, 109, 634, 868, 1099
DdeI CTNAG 4 cut(s) 26, 267, 936, 981
DpnI GATC 1 cut(s) 127
DpnII GATC 1 cut(s) 125
Eam1104I CTCTTC 2 cut(s) 414, 1058
EarI CTCTTC 2 cut(s) 414, 1058
Eco147I AGGCCT 1 cut(s) 406
Eco24I GRGCYC 1 cut(s) 16
Eco47I GGWCC 2 cut(s) 91, 617
Eco57I CTGAAG 1 cut(s) 468
EcoRII CCWGG 1 cut(s) 121
EcoT38I GRGCYC 1 cut(s) 16
FaeI CATG 3 cut(s) 91, 518, 564
FatI CATG 3 cut(s) 87, 514, 560
FblI GTMKAC 1 cut(s) 911
Fnu4HI GCNGC 5 cut(s) 316, 319, 374, 420, 512
FokI GGATG 3 cut(s) 262, 535, 568
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 5 cut(s) 316, 319, 374, 420, 512
FspBI CTAG 6 cut(s) 45, 210, 566, 1035, 1073, 1114
GluI GCNGC 5 cut(s) 316, 319, 374, 420, 512
HaeIII GGCC 4 cut(s) 161, 406, 676, 942
HapII CCGG 2 cut(s) 94, 638
HgaI GACGC 1 cut(s) 163
Hin1I GRCGYC 2 cut(s) 39, 174
Hin1II CATG 3 cut(s) 91, 518, 564
HincII GTYRAC 2 cut(s) 85, 1057
HindII GTYRAC 2 cut(s) 85, 1057
HinfI GANTC 6 cut(s) 269, 431, 548, 593, 893, 977
HpaI GTTAAC 2 cut(s) 85, 1057
HpaII CCGG 2 cut(s) 94, 638
HphI GGTGA 4 cut(s) 208, 551, 652, 838
Hpy166II GTNNAC 3 cut(s) 85, 912, 1057
Hpy188I TCNGA 5 cut(s) 29, 147, 268, 448, 790
Hpy188III TCNNGA 3 cut(s) 620, 923, 1073
Hpy8I GTNNAC 3 cut(s) 85, 912, 1057
Hpy99I CGWCG 4 cut(s) 109, 173, 179, 300
HpyAV CCTTC 6 cut(s) 3, 329, 443, 608, 739, 920
HpyCH4III ACNGT 2 cut(s) 82, 699
HpyCH4IV ACGT 2 cut(s) 39, 797
HpyCH4V TGCA 4 cut(s) 321, 514, 684, 803
HpyF10VI GCNNNNNNNGC 1 cut(s) 1040
HpyF3I CTNAG 4 cut(s) 26, 267, 936, 981
HpySE526I ACGT 2 cut(s) 39, 797
Hsp92I GRCGYC 2 cut(s) 39, 174
Hsp92II CATG 3 cut(s) 91, 518, 564
KpnI GGTACC 1 cut(s) 637
KspAI GTTAAC 2 cut(s) 85, 1057
Kzo9I GATC 1 cut(s) 125
Lsp1109I GCAGC 4 cut(s) 305, 385, 406, 498
LweI GCATC 2 cut(s) 513, 693
MaeI CTAG 6 cut(s) 45, 210, 566, 1035, 1073, 1114
MaeII ACGT 2 cut(s) 39, 797
MaeIII GTNAC 3 cut(s) 40, 640, 997
MalI GATC 1 cut(s) 127
MboI GATC 1 cut(s) 125
MhlI GDGCHC 1 cut(s) 16
MluCI AATT 6 cut(s) 130, 204, 586, 718, 764, 965
MlyI GAGTC 1 cut(s) 278
MnlI CCTC 3 cut(s) 417, 705, 844
MseI TTAA 5 cut(s) 84, 255, 603, 767, 1056
MspA1I CMGCKG 2 cut(s) 318, 974
MspI CCGG 2 cut(s) 94, 638
MspR9I CCNGG 2 cut(s) 123, 638
MvaI CCWGG 1 cut(s) 123
MwoI GCNNNNNNNGC 1 cut(s) 1040
NciI CCSGG 1 cut(s) 638
NdeII GATC 1 cut(s) 125
NlaIII CATG 3 cut(s) 91, 518, 564
NlaIV GGNNCC 4 cut(s) 160, 306, 635, 751
NmuCI GTSAC 2 cut(s) 40, 640
PceI AGGCCT 1 cut(s) 406
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PfeI GAWTC 5 cut(s) 431, 548, 593, 893, 977
PflFI GACNNNGTC 1 cut(s) 173
PinAI ACCGGT 1 cut(s) 93
PkrI GCNGC 5 cut(s) 317, 320, 375, 421, 513
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 4 cut(s) 160, 306, 635, 751
PspPI GGNCC 5 cut(s) 91, 159, 617, 674, 940
PsyI GACNNNGTC 1 cut(s) 173
PvuII CAGCTG 1 cut(s) 974
RsaI GTAC 5 cut(s) 22, 110, 635, 869, 1100
RsaNI GTAC 5 cut(s) 21, 109, 634, 868, 1099
SaqAI TTAA 5 cut(s) 84, 255, 603, 767, 1056
SatI GCNGC 5 cut(s) 316, 319, 374, 420, 512
Sau3AI GATC 1 cut(s) 125
Sau96I GGNCC 5 cut(s) 91, 159, 617, 674, 940
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 2 cut(s) 123, 638
SduI GDGCHC 1 cut(s) 16
SfaNI GCATC 2 cut(s) 513, 693
SfcI CTRYAG 1 cut(s) 244
SinI GGWCC 2 cut(s) 91, 617
SmlI CTYRAG 1 cut(s) 921
SmoI CTYRAG 1 cut(s) 921
Sse9I AATT 6 cut(s) 130, 204, 586, 718, 764, 965
SseBI AGGCCT 1 cut(s) 406
SsiI CCGC 4 cut(s) 58, 76, 316, 329
SspMI CTAG 6 cut(s) 45, 210, 566, 1035, 1073, 1114
StuI AGGCCT 1 cut(s) 406
StyD4I CCNGG 2 cut(s) 121, 636
TaaI ACNGT 2 cut(s) 82, 699
TaiI ACGT 2 cut(s) 42, 800
TaqI TCGA 1 cut(s) 128
TaqII GACCGA 2 cut(s) 252, 722
TasI AATT 6 cut(s) 130, 204, 586, 718, 764, 965
TatI WGTACW 1 cut(s) 20
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 5 cut(s) 431, 548, 593, 893, 977
Tru1I TTAA 5 cut(s) 84, 255, 603, 767, 1056
Tru9I TTAA 5 cut(s) 84, 255, 603, 767, 1056
TseFI GTSAC 2 cut(s) 40, 640
TseI GCWGC 4 cut(s) 318, 373, 419, 511
Tsp45I GTSAC 2 cut(s) 40, 640
TspDTI ATGAA 7 cut(s) 206, 360, 480, 567, 585, 906, 975
TspGWI ACGGA 1 cut(s) 93
Tth111I GACNNNGTC 1 cut(s) 173
VpaK11BI GGWCC 2 cut(s) 91, 617
XapI RAATTY 2 cut(s) 130, 965
XbaI TCTAGA 1 cut(s) 1072
XcmI CCANNNNNNNNNTGG 1 cut(s) 627
XmiI GTMKAC 1 cut(s) 911
XspI CTAG 6 cut(s) 45, 210, 566, 1035, 1073, 1114
ZraI GACGTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.