Rorug06G0151600

Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013250
Physical Location & Seq
Reverse (-)
21723361 .. 21723895
535 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0151600.1

Sequence Viewer

Length: 444 bp
ATGGAGTCATGCCTTACCATAAGGAACATCCTCCTCCTAGATTCTGAAGGAAAACGTATAGCTGTCAAGTATTATTCAGAGGACTGGCCAACGAATAGTGCAAAAGAAGCTTTTGAGAAGGCCACCTTCACTTTTTTGTTACCGGGGGGTGATAATGAAATTGAGCTCATTTTAGCCACAGTTCTTCAGGGGTTTTTTGATGCAGTTGGAATTCTCCTCAGAGGCAATGTGGATAAAAAGGAGGCACTTGAGTATTTGGACCTCATTCTACTATGCCTTCATGAAATTGTTGATGGCACCATTGTTCTTGAGACCGATTCAAATGTTATAGCAAGTAAGGTTGCGAGTCATAGTATTGATTCTGGAGCTCCGTTATCTGAGCAGACAATAGGCCAAGCACTTGCTACTGCACGCGAACATTTTGCAAGATCTCTTCTTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRAP

Protein Analysis

147

Amino Acids

16.11

Weight (kDa)

4.72

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 41 - 116 6.5e-06 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26470 AT2G26470
fragaria_vesca FvH4_2g17880 FvH4_2g17880 FvH4_2g17880
malus_domestica MD05G1163100.v1.1
prunus_persica Prupe.8G191400_v2.0.a1
pyrus_communis pycom05g15260
rosa_chinensis RchiOBHm_Chr6g0283131
rosa_laevigata RLG00000012842
rosa_roxburghii Rroxscaffold_7G00185750
rosa_rugosa Rorug06G0151500.1 Rorug06G0151600
rosa_samantha Rh6AG264400 Rh6BG267300 Rh6CG266300 Rh6DG259600
rosa_wichuraiana Rw6G022880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 296
AccII CGCG 1 cut(s) 414
AcoI YGGCCR 1 cut(s) 86
AcsI RAATTY 1 cut(s) 210
AcuI CTGAAG 2 cut(s) 66, 170
AflII CTTAAG 1 cut(s) 437
AgsI TTSAA 1 cut(s) 321
AluBI AGCT 4 cut(s) 62, 110, 166, 368
AluI AGCT 4 cut(s) 62, 110, 166, 368
Alw21I GWGCWC 2 cut(s) 168, 370
Alw26I GTCTC 1 cut(s) 305
AoxI GGCC 3 cut(s) 86, 120, 391
ApoI RAATTY 1 cut(s) 210
AspS9I GGNCC 1 cut(s) 259
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 1 cut(s) 161
AvaII GGWCC 1 cut(s) 259
BalI TGGCCA 1 cut(s) 88
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 2 cut(s) 168, 370
Bbv12I GWGCWC 2 cut(s) 168, 370
BccI CCATC 1 cut(s) 287
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 305
BfaI CTAG 1 cut(s) 38
BfrI CTTAAG 1 cut(s) 437
BglII AGATCT 1 cut(s) 428
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 1 cut(s) 144
BmsI GCATC 1 cut(s) 190
BpmI CTGGAG 1 cut(s) 384
BpuEI CTTGAG 2 cut(s) 269, 329
BpuMI CCSGG 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 143
Bse1I ACTGG 1 cut(s) 89
Bse3DI GCAATG 1 cut(s) 232
BseDI CCNNGG 1 cut(s) 143
BseGI GGATG 1 cut(s) 27
BseMI GCAATG 1 cut(s) 232
BseMII CTCAG 2 cut(s) 232, 369
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 2 cut(s) 23, 206
BsgI GTGCAG 1 cut(s) 393
Bsh1236I CGCG 1 cut(s) 414
BshFI GGCC 3 cut(s) 88, 122, 393
BshNI GGYRCC 1 cut(s) 296
BsiHKAI GWGCWC 2 cut(s) 168, 370
BsiSI CCGG 1 cut(s) 143
BsmAI GTCTC 1 cut(s) 305
BsnI GGCC 3 cut(s) 88, 122, 393
Bso31I GGTCTC 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 168, 370
Bsp143I GATC 1 cut(s) 428
BspANI GGCC 3 cut(s) 88, 122, 393
BspCNI CTCAG 2 cut(s) 231, 370
BspFNI CGCG 1 cut(s) 414
BspHI TCATGA 1 cut(s) 280
BspLI GGNNCC 1 cut(s) 298
BspT107I GGYRCC 1 cut(s) 296
BspTI CTTAAG 1 cut(s) 437
BspTNI GGTCTC 1 cut(s) 305
BsrDI GCAATG 1 cut(s) 232
BsrI ACTGG 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 143
BssMI GATC 1 cut(s) 428
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 1 cut(s) 438
BstAFI CTTAAG 1 cut(s) 437
BstC8I GCNNGC 1 cut(s) 412
BstDEI CTNAG 2 cut(s) 218, 378
BstF5I GGATG 1 cut(s) 27
BstFNI CGCG 1 cut(s) 414
BstKTI GATC 1 cut(s) 431
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 1 cut(s) 428
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 142
BstUI CGCG 1 cut(s) 414
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
BsuRI GGCC 3 cut(s) 88, 122, 393
BtsCI GGATG 1 cut(s) 27
Cac8I GCNNGC 1 cut(s) 412
CciI TCATGA 1 cut(s) 280
Cfr13I GGNCC 1 cut(s) 259
CviAII CATG 2 cut(s) 9, 281
CviJI RGCY 8 cut(s) 62, 88, 110, 122, 166, 176, 368, 393
CviKI_1 RGCY 8 cut(s) 62, 88, 110, 122, 166, 176, 368, 393
DdeI CTNAG 2 cut(s) 218, 378
DpnI GATC 1 cut(s) 430
DpnII GATC 1 cut(s) 428
EaeI YGGCCR 1 cut(s) 86
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Ecl136II GAGCTC 2 cut(s) 166, 368
Eco24I GRGCYC 2 cut(s) 168, 370
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 259
Eco53kI GAGCTC 2 cut(s) 166, 368
Eco57I CTGAAG 2 cut(s) 66, 170
EcoICRI GAGCTC 2 cut(s) 166, 368
EcoRI GAATTC 1 cut(s) 210
EcoT38I GRGCYC 2 cut(s) 168, 370
FaeI CATG 2 cut(s) 12, 284
FaiI YATR 7 cut(s) 10, 20, 59, 274, 282, 329, 351
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FatI CATG 2 cut(s) 8, 280
FokI GGATG 1 cut(s) 14
FriOI GRGCYC 2 cut(s) 168, 370
FspBI CTAG 1 cut(s) 38
GsuI CTGGAG 1 cut(s) 384
HaeIII GGCC 3 cut(s) 88, 122, 393
HapII CCGG 1 cut(s) 143
Hin1II CATG 2 cut(s) 12, 284
HindIII AAGCTT 1 cut(s) 108
HinfI GANTC 5 cut(s) 5, 41, 317, 346, 359
HpaII CCGG 1 cut(s) 143
HphI GGTGA 1 cut(s) 161
Hpy188I TCNGA 4 cut(s) 46, 79, 221, 379
Hpy188III TCNNGA 3 cut(s) 281, 308, 363
HpyAV CCTTC 4 cut(s) 41, 112, 136, 287
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 55
HpyCH4V TGCA 4 cut(s) 101, 203, 410, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 2 cut(s) 218, 378
HpySE526I ACGT 1 cut(s) 55
Hsp92II CATG 2 cut(s) 12, 284
Kzo9I GATC 1 cut(s) 428
LmnI GCTCC 2 cut(s) 365, 373
LpnPI CCDG 4 cut(s) 70, 156, 173, 348
LweI GCATC 1 cut(s) 190
MaeI CTAG 1 cut(s) 38
MaeII ACGT 1 cut(s) 55
MaeIII GTNAC 1 cut(s) 138
MalI GATC 1 cut(s) 430
MboI GATC 1 cut(s) 428
MboII GAAGA 2 cut(s) 176, 425
MflI RGATCY 1 cut(s) 428
MhlI GDGCHC 2 cut(s) 168, 370
MlsI TGGCCA 1 cut(s) 88
MluCI AATT 3 cut(s) 159, 210, 285
MluNI TGGCCA 1 cut(s) 88
MlyI GAGTC 2 cut(s) 14, 355
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 7 cut(s) 41, 44, 73, 215, 227, 235, 272
Mox20I TGGCCA 1 cut(s) 88
MscI TGGCCA 1 cut(s) 88
MseI TTAA 1 cut(s) 438
Msp20I TGGCCA 1 cut(s) 88
MspCI CTTAAG 1 cut(s) 437
MspI CCGG 1 cut(s) 143
MspR9I CCNGG 1 cut(s) 144
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 1 cut(s) 107
NciI CCSGG 1 cut(s) 144
NdeII GATC 1 cut(s) 428
NlaIII CATG 2 cut(s) 12, 284
NlaIV GGNNCC 1 cut(s) 298
PagI TCATGA 1 cut(s) 280
PfeI GAWTC 3 cut(s) 41, 317, 359
PleI GAGTC 2 cut(s) 13, 354
PpsI GAGTC 2 cut(s) 13, 354
Psp124BI GAGCTC 2 cut(s) 168, 370
PspN4I GGNNCC 1 cut(s) 298
PspPI GGNCC 1 cut(s) 259
PsuI RGATCY 1 cut(s) 428
SacI GAGCTC 2 cut(s) 168, 370
SaqAI TTAA 1 cut(s) 438
Sau3AI GATC 1 cut(s) 428
Sau96I GGNCC 1 cut(s) 259
SchI GAGTC 2 cut(s) 14, 355
ScrFI CCNGG 1 cut(s) 144
SduI GDGCHC 2 cut(s) 168, 370
SetI ASST 8 cut(s) 58, 64, 112, 128, 168, 264, 342, 370
SfaNI GCATC 1 cut(s) 190
SinI GGWCC 1 cut(s) 259
SmlI CTYRAG 3 cut(s) 248, 308, 437
SmoI CTYRAG 3 cut(s) 248, 308, 437
Sse9I AATT 3 cut(s) 159, 210, 285
SspMI CTAG 1 cut(s) 38
SstI GAGCTC 2 cut(s) 168, 370
StyD4I CCNGG 1 cut(s) 142
TaaI ACNGT 1 cut(s) 181
TaiI ACGT 1 cut(s) 58
TaqII GACCGA 1 cut(s) 329
TasI AATT 3 cut(s) 159, 210, 285
TfiI GAWTC 3 cut(s) 41, 317, 359
Tru1I TTAA 1 cut(s) 438
Tru9I TTAA 1 cut(s) 438
TspDTI ATGAA 3 cut(s) 171, 269, 297
TspGWI ACGGA 1 cut(s) 360
Vha464I CTTAAG 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 259
XapI RAATTY 1 cut(s) 210
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.