Prupe.8G191400_v2.0.a1

Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0013250
Physical Location & Seq
Forward (+)
18581539 .. 18584634
3096 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G191400.1

Sequence Viewer

Length: 1092 bp
ATGTGTGGGAGGGCTCGCTGTACTCTCAGAGCGGACGACATCCCTAGGGCTTGCCACCGCAGCCATGGCCCCGTCCGTACTGTTAACATGGACCGGTTTCGCCCGCTGTTCAATGCGTCGCCGGGGTCGAATTTGCCTGTTGTTCGTAGAGAAGACGGAGGAGACGGCGACGGCGTTGTTGTTCACTGTATGAAATGGGGGCTGATCCCTAGTTTCACCAAGAAAACCGAGAAGCCCGATCACTATAAGATGTTTAATGCTAGGTCTGAGTCCATTTGCGAAAAGGCTTCTTTTCGACGTCTAATTCCTAAAAACCGCTGCCTCATTGCAGTTGAAGGATTTTATGAATGGAAAAAAGATGGTTCTAAAAAGCAGCCTTACTACGTCCACTTTAATGACGGCCGACCGCTTCTGTTTGCTGCTCTTTATGATTTTTGGGAGAACTCTGAAGGCGAGAAACTTTACACCTTCACTATCATTACAACGTCTTCATCTTCAGCTCTAGGGTGGCTGCATGACAGGATGCCTGTTATTTTGGGTGACAAGGGATCTACCGACTCATGGCTAAGTGGTTCTTCAACTTCCAATTTTGATTCATTACTTAAACCATATGAAGGTCCAGATTTGGTATGGTACCCGGTGACACAAGCAATGGGCAAGGTGTCTTTTGATGGACCAGAGTGCATCAATGAGATACAGCTGAAGACAGAGGGGAATAATTCAATCACAAAGTTTTTCATGTCGAAAGGAACCAAAAAAGAAGAATTAAACCCAAAGGACACTAGTTTTTACGACTCATCTGTGAAGAATGATTTGCCAAAAAGCGTGAAGGAAGAACCTGAAGGCAAAGAGAAAACAGAACAACCAGCCTCTACAGAAAAATGTGAGAATGATTCTAAGGGCCAAACAATATCGCAAGAGGGTGTATCTAAGGGCCAAACAAAGCGAGACTATGAAGAGTTTTCGGCTGACTCTAAGCCAGTTGCTTATGAAACTAGTGAGATGTCTGCTAGCCCAGCAAAGAAGAAGGTTAACCCGAAGAGTTCTGTTGACAAGCAACCTACCCTATTTTCGTACTTCGGAAAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRAP

Protein Analysis

364

Amino Acids

40.79

Weight (kDa)

8.44

Isoelectric Point (pI)

52.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26470 AT2G26470
fragaria_vesca FvH4_2g17880 FvH4_2g17880 FvH4_2g17880
malus_domestica MD05G1163100.v1.1
prunus_persica Prupe.8G191400_v2.0.a1
pyrus_communis pycom05g15260
rosa_chinensis RchiOBHm_Chr6g0283131
rosa_laevigata RLG00000012842
rosa_roxburghii Rroxscaffold_7G00185750
rosa_rugosa Rorug06G0151500.1 Rorug06G0151600
rosa_samantha Rh6AG264400 Rh6BG267300 Rh6CG266300 Rh6DG259600
rosa_wichuraiana Rw6G022880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 301
Acc65I GGTACC 1 cut(s) 633
AccB1I GGYRCC 1 cut(s) 633
AccBSI CCGCTC 1 cut(s) 32
AciI CCGC 5 cut(s) 32, 58, 104, 316, 407
AclWI GGATC 2 cut(s) 199, 556
AcoI YGGCCR 1 cut(s) 400
AcsI RAATTY 1 cut(s) 130
AcuI CTGAAG 4 cut(s) 468, 480, 722, 861
AcyI GRCGYC 1 cut(s) 298
AfaI GTAC 4 cut(s) 22, 79, 635, 1076
AfiI CCNNNNNNNGG 2 cut(s) 561, 614
AgeI ACCGGT 1 cut(s) 93
AgsI TTSAA 4 cut(s) 112, 335, 579, 723
AhlI ACTAGT 2 cut(s) 782, 995
AluBI AGCT 3 cut(s) 500, 700, 1089
AluI AGCT 3 cut(s) 500, 700, 1089
Alw26I GTCTC 2 cut(s) 156, 942
AlwI GGATC 2 cut(s) 199, 556
AoxI GGCC 4 cut(s) 67, 400, 901, 934
ApeKI GCWGC 5 cut(s) 60, 318, 373, 419, 511
ApoI RAATTY 1 cut(s) 130
AsiGI ACCGGT 1 cut(s) 93
Asp718I GGTACC 1 cut(s) 633
AspA2I CCTAGG 1 cut(s) 44
AspS9I GGNCC 6 cut(s) 68, 91, 617, 674, 901, 934
AsuC2I CCSGG 2 cut(s) 123, 638
AsuHPI GGTGA 3 cut(s) 208, 551, 652
AsuNHI GCTAGC 1 cut(s) 1010
AvaII GGWCC 3 cut(s) 91, 617, 674
AvrII CCTAGG 1 cut(s) 44
BanI GGYRCC 1 cut(s) 633
BanII GRGCYC 1 cut(s) 16
BbsI GAAGAC 3 cut(s) 159, 480, 710
BbvI GCAGC 5 cut(s) 72, 305, 385, 406, 498
BccI CCATC 2 cut(s) 353, 665
BceAI ACGGC 3 cut(s) 181, 187, 415
BcnI CCSGG 2 cut(s) 123, 638
BcoDI GTCTC 2 cut(s) 156, 942
BcuI ACTAGT 2 cut(s) 782, 995
BfaI CTAG 8 cut(s) 45, 210, 261, 503, 783, 996, 1011, 1090
BfmI CTRYAG 1 cut(s) 873
BisI GCNGC 5 cut(s) 61, 319, 374, 420, 512
BlnI CCTAGG 1 cut(s) 44
BlsI GCNGC 5 cut(s) 62, 320, 375, 421, 513
Bme1390I CCNGG 2 cut(s) 123, 638
Bme18I GGWCC 3 cut(s) 91, 617, 674
BmgT120I GGNCC 6 cut(s) 68, 91, 617, 674, 901, 934
BmiI GGNNCC 3 cut(s) 70, 635, 751
BmrFI CCNGG 2 cut(s) 123, 638
BmsI GCATC 2 cut(s) 513, 693
BmtI GCTAGC 1 cut(s) 1014
BpiI GAAGAC 3 cut(s) 159, 480, 710
BpuMI CCSGG 2 cut(s) 123, 638
BsaHI GRCGYC 1 cut(s) 298
BsaJI CCNNGG 3 cut(s) 44, 64, 122
BsaWI WCCGGW 1 cut(s) 93
Bsc4I CCNNNNNNNGG 2 cut(s) 561, 614
Bse118I RCCGGY 1 cut(s) 93
Bse1I ACTGG 1 cut(s) 980
Bse3DI GCAATG 2 cut(s) 324, 657
BseDI CCNNGG 3 cut(s) 44, 64, 122
BseGI GGATG 2 cut(s) 39, 528
BseLI CCNNNNNNNGG 2 cut(s) 561, 614
BseMI GCAATG 2 cut(s) 324, 657
BseMII CTCAG 2 cut(s) 40, 258
BseNI ACTGG 1 cut(s) 980
BseRI GAGGAG 1 cut(s) 174
BseX3I CGGCCG 1 cut(s) 400
BseXI GCAGC 5 cut(s) 72, 305, 385, 406, 498
BseYI CCCAGC 1 cut(s) 1015
Bsh1285I CGRYCG 2 cut(s) 403, 407
BshFI GGCC 4 cut(s) 69, 402, 903, 936
BshNI GGYRCC 1 cut(s) 633
BshTI ACCGGT 1 cut(s) 93
BsiEI CGRYCG 2 cut(s) 403, 407
BsiSI CCGG 3 cut(s) 94, 122, 638
BslI CCNNNNNNNGG 2 cut(s) 561, 614
BsmAI GTCTC 2 cut(s) 156, 942
BsmBI CGTCTC 1 cut(s) 156
BsnI GGCC 4 cut(s) 69, 402, 903, 936
Bsp1286I GDGCHC 1 cut(s) 16
Bsp143I GATC 3 cut(s) 204, 238, 548
Bsp19I CCATGG 1 cut(s) 64
BspACI CCGC 5 cut(s) 32, 58, 104, 316, 407
BspANI GGCC 4 cut(s) 69, 402, 903, 936
BspCNI CTCAG 2 cut(s) 39, 259
BspLI GGNNCC 3 cut(s) 70, 635, 751
BspOI GCTAGC 1 cut(s) 1014
BspPI GGATC 2 cut(s) 199, 556
BspT107I GGYRCC 1 cut(s) 633
BsrBI CCGCTC 1 cut(s) 32
BsrDI GCAATG 2 cut(s) 324, 657
BsrFI RCCGGY 1 cut(s) 93
BsrI ACTGG 1 cut(s) 980
BssAI RCCGGY 1 cut(s) 93
BssECI CCNNGG 3 cut(s) 44, 64, 122
BssMI GATC 3 cut(s) 204, 238, 548
BssNI GRCGYC 1 cut(s) 298
BssT1I CCWWGG 2 cut(s) 44, 64
Bst4CI ACNGT 2 cut(s) 82, 188
Bst6I CTCTTC 2 cut(s) 951, 1034
BstACI GRCGYC 1 cut(s) 298
BstC8I GCNNGC 4 cut(s) 16, 52, 104, 1012
BstDEI CTNAG 6 cut(s) 26, 267, 566, 897, 930, 975
BstDSI CCRYGG 1 cut(s) 64
BstF5I GGATG 2 cut(s) 39, 528
BstKTI GATC 3 cut(s) 207, 241, 551
BstMAI GTCTC 2 cut(s) 156, 942
BstMBI GATC 3 cut(s) 204, 238, 548
BstMCI CGRYCG 2 cut(s) 403, 407
BstMWI GCNNNNNNNGC 3 cut(s) 60, 66, 1016
BstSCI CCNGG 2 cut(s) 121, 636
BstSFI CTRYAG 1 cut(s) 873
BstV1I GCAGC 5 cut(s) 72, 305, 385, 406, 498
BstV2I GAAGAC 3 cut(s) 159, 480, 710
BstX2I RGATCY 1 cut(s) 548
BstYI RGATCY 1 cut(s) 548
BstZI CGGCCG 1 cut(s) 400
BsuRI GGCC 4 cut(s) 69, 402, 903, 936
BtgI CCRYGG 1 cut(s) 64
BtsCI GGATG 2 cut(s) 39, 528
BtsIMutI CAGTG 1 cut(s) 184
Cac8I GCNNGC 4 cut(s) 16, 52, 104, 1012
Cfr10I RCCGGY 1 cut(s) 93
Cfr13I GGNCC 6 cut(s) 68, 91, 617, 674, 901, 934
CseI GACGC 1 cut(s) 105
Csp6I GTAC 4 cut(s) 21, 78, 634, 1075
CspAI ACCGGT 1 cut(s) 93
CviAII CATG 5 cut(s) 65, 88, 515, 561, 739
CviQI GTAC 4 cut(s) 21, 78, 634, 1075
DdeI CTNAG 6 cut(s) 26, 267, 566, 897, 930, 975
DpnI GATC 3 cut(s) 206, 240, 550
DpnII GATC 3 cut(s) 204, 238, 548
EaeI YGGCCR 1 cut(s) 400
EagI CGGCCG 1 cut(s) 400
Eam1104I CTCTTC 2 cut(s) 951, 1034
EarI CTCTTC 2 cut(s) 951, 1034
EclXI CGGCCG 1 cut(s) 400
Eco130I CCWWGG 2 cut(s) 44, 64
Eco24I GRGCYC 1 cut(s) 16
Eco47I GGWCC 3 cut(s) 91, 617, 674
Eco52I CGGCCG 1 cut(s) 400
Eco57I CTGAAG 4 cut(s) 468, 480, 722, 861
EcoT14I CCWWGG 2 cut(s) 44, 64
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 2 cut(s) 44, 64
Esp3I CGTCTC 1 cut(s) 156
FaeI CATG 5 cut(s) 68, 91, 518, 564, 742
FalI AAGNNNNNCTT 2 cut(s) 559, 591
FatI CATG 5 cut(s) 64, 87, 514, 560, 738
FauI CCCGC 1 cut(s) 111
FauNDI CATATG 1 cut(s) 610
Fnu4HI GCNGC 5 cut(s) 61, 319, 374, 420, 512
FokI GGATG 2 cut(s) 26, 535
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 5 cut(s) 61, 319, 374, 420, 512
FspBI CTAG 8 cut(s) 45, 210, 261, 503, 783, 996, 1011, 1090
GluI GCNGC 5 cut(s) 61, 319, 374, 420, 512
GsaI CCCAGC 1 cut(s) 1019
HaeIII GGCC 4 cut(s) 69, 402, 903, 936
HapII CCGG 3 cut(s) 94, 122, 638
HgaI GACGC 1 cut(s) 105
Hin1I GRCGYC 1 cut(s) 298
Hin1II CATG 5 cut(s) 68, 91, 518, 564, 742
HincII GTYRAC 3 cut(s) 85, 1033, 1051
HindII GTYRAC 3 cut(s) 85, 1033, 1051
HinfI GANTC 6 cut(s) 269, 557, 593, 794, 893, 971
HpaI GTTAAC 2 cut(s) 85, 1033
HpaII CCGG 3 cut(s) 94, 122, 638
HphI GGTGA 3 cut(s) 208, 551, 652
Hpy166II GTNNAC 5 cut(s) 85, 184, 388, 1033, 1051
Hpy188I TCNGA 4 cut(s) 29, 268, 448, 1082
Hpy188III TCNNGA 1 cut(s) 620
Hpy8I GTNNAC 5 cut(s) 85, 184, 388, 1033, 1051
Hpy99I CGWCG 3 cut(s) 121, 173, 300
HpyAV CCTTC 7 cut(s) 329, 443, 478, 608, 823, 836, 1021
HpyCH4III ACNGT 2 cut(s) 82, 188
HpyCH4IV ACGT 3 cut(s) 298, 384, 485
HpyCH4V TGCA 3 cut(s) 329, 514, 684
HpyF10VI GCNNNNNNNGC 3 cut(s) 60, 66, 1016
HpyF3I CTNAG 6 cut(s) 26, 267, 566, 897, 930, 975
HpySE526I ACGT 3 cut(s) 298, 384, 485
Hsp92I GRCGYC 1 cut(s) 298
Hsp92II CATG 5 cut(s) 68, 91, 518, 564, 742
KpnI GGTACC 1 cut(s) 637
KspAI GTTAAC 2 cut(s) 85, 1033
Kzo9I GATC 3 cut(s) 204, 238, 548
Lsp1109I GCAGC 5 cut(s) 72, 305, 385, 406, 498
LweI GCATC 2 cut(s) 513, 693
MaeI CTAG 8 cut(s) 45, 210, 261, 503, 783, 996, 1011, 1090
MaeII ACGT 3 cut(s) 298, 384, 485
MaeIII GTNAC 2 cut(s) 539, 640
MalI GATC 3 cut(s) 206, 240, 550
MbiI CCGCTC 1 cut(s) 32
MboI GATC 3 cut(s) 204, 238, 548
MflI RGATCY 1 cut(s) 548
MhlI GDGCHC 1 cut(s) 16
MluCI AATT 5 cut(s) 130, 303, 586, 718, 764
MlyI GAGTC 4 cut(s) 278, 551, 788, 965
MnlI CCTC 6 cut(s) 3, 152, 332, 703, 880, 913
MseI TTAA 6 cut(s) 84, 255, 393, 603, 767, 1032
MslI CAYNNNNRTG 1 cut(s) 393
MspA1I CMGCKG 3 cut(s) 106, 318, 700
MspI CCGG 3 cut(s) 94, 122, 638
MspR9I CCNGG 2 cut(s) 123, 638
MwoI GCNNNNNNNGC 3 cut(s) 60, 66, 1016
NciI CCSGG 2 cut(s) 123, 638
NcoI CCATGG 1 cut(s) 64
NdeI CATATG 1 cut(s) 610
NdeII GATC 3 cut(s) 204, 238, 548
NheI GCTAGC 1 cut(s) 1010
NlaIII CATG 5 cut(s) 68, 91, 518, 564, 742
NlaIV GGNNCC 3 cut(s) 70, 635, 751
NmuCI GTSAC 2 cut(s) 539, 640
PcsI WCGNNNNNNNCGW 2 cut(s) 125, 171
PfeI GAWTC 2 cut(s) 593, 893
PinAI ACCGGT 1 cut(s) 93
PkrI GCNGC 5 cut(s) 62, 320, 375, 421, 513
PleI GAGTC 4 cut(s) 277, 551, 788, 965
PpsI GAGTC 4 cut(s) 277, 551, 788, 965
PspFI CCCAGC 1 cut(s) 1015
PspN4I GGNNCC 3 cut(s) 70, 635, 751
PspPI GGNCC 6 cut(s) 68, 91, 617, 674, 901, 934
PsuI RGATCY 1 cut(s) 548
PvuII CAGCTG 1 cut(s) 700
RsaI GTAC 4 cut(s) 22, 79, 635, 1076
RsaNI GTAC 4 cut(s) 21, 78, 634, 1075
RseI CAYNNNNRTG 1 cut(s) 393
SaqAI TTAA 6 cut(s) 84, 255, 393, 603, 767, 1032
SatI GCNGC 5 cut(s) 61, 319, 374, 420, 512
Sau3AI GATC 3 cut(s) 204, 238, 548
Sau96I GGNCC 6 cut(s) 68, 91, 617, 674, 901, 934
SchI GAGTC 4 cut(s) 278, 551, 788, 965
ScrFI CCNGG 2 cut(s) 123, 638
SduI GDGCHC 1 cut(s) 16
SfaNI GCATC 2 cut(s) 513, 693
SfcI CTRYAG 1 cut(s) 873
SinI GGWCC 3 cut(s) 91, 617, 674
SmiMI CAYNNNNRTG 1 cut(s) 393
SpeI ACTAGT 2 cut(s) 782, 995
Sse9I AATT 5 cut(s) 130, 303, 586, 718, 764
SsiI CCGC 5 cut(s) 32, 58, 104, 316, 407
SspMI CTAG 8 cut(s) 45, 210, 261, 503, 783, 996, 1011, 1090
StyD4I CCNGG 2 cut(s) 121, 636
StyI CCWWGG 2 cut(s) 44, 64
TaaI ACNGT 2 cut(s) 82, 188
TaiI ACGT 3 cut(s) 301, 387, 488
TaqI TCGA 3 cut(s) 128, 295, 743
TasI AATT 5 cut(s) 130, 303, 586, 718, 764
TatI WGTACW 1 cut(s) 20
TfiI GAWTC 2 cut(s) 593, 893
Tru1I TTAA 6 cut(s) 84, 255, 393, 603, 767, 1032
Tru9I TTAA 6 cut(s) 84, 255, 393, 603, 767, 1032
TscAI CASTG 1 cut(s) 191
TseFI GTSAC 2 cut(s) 539, 640
TseI GCWGC 5 cut(s) 60, 318, 373, 419, 511
Tsp45I GTSAC 2 cut(s) 539, 640
TspDTI ATGAA 8 cut(s) 206, 360, 480, 585, 627, 727, 969, 1005
TspGWI ACGGA 2 cut(s) 65, 171
TspRI CASTG 1 cut(s) 191
VpaK11BI GGWCC 3 cut(s) 91, 617, 674
XapI RAATTY 1 cut(s) 130
XcmI CCANNNNNNNNNTGG 2 cut(s) 62, 627
XmaJI CCTAGG 1 cut(s) 44
XspI CTAG 8 cut(s) 45, 210, 261, 503, 783, 996, 1011, 1090
ZraI GACGTC 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.