Rh6BG267300

Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0013250
Physical Location & Seq
Forward (+)
48427901 .. 48431158
3258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG267300.1

Sequence Viewer

Length: 1107 bp
ATGTGTGGGAGGGCGCGCTGTACTCTCAGAGCCGACGACGTTTCTAGGGCTTGCTACCGCAACCATGGCGCCGTTCGTACGATTAACATGGACCGGTATCGGCCGTCGTATAATGTGTCGCCGGGAGCGGATTTTCCGGTCGTGCGTAGAGGAGATGGTGGGTCAGATGGTGAAGACGGCGTTGTTCTTCATTGCATGAAATGGGGACTGATTCCGAGTTTCTCCAAGAAAACGGAGAAGCCCGATCATTACAGAATGTTCAATGCTCGCTCTGAGTCCATTTGTGAAAAGGCTTCTTTTCGTCGTCTAGTTCCTAAAAACCGCTGCCTAGTTGCTGTAGAAGGATTTTATGAATGGAAAAAAGATGGTTCTAAGAAACAGCCTTACTATGTCCATTTTAAGGATGGTCGGCCTCTTCTGTTTGCTGCTCTTTATGATTCGTGGGAGAATTCTGAAGGTGAGAAACTTTATACCTTCACTATCATTACAACTTCTTCATCTTCAGCTTTGGCGTGGCTCCATGACAGGATGCCAGTTATTTTGGGTGACAAGGAATCTATTGATACATGGCTTAATGATTCTTCATCTTCCAATTTTGACAAGTTACTTAAACCATATGAAGGGTCTGATTTGGTATGGTACCCAGTAACACCAGCAATGGGTAAGGTGTCTTTTAATGGGCCAGAGTGCAGCAATGAGATACAGTTGAAGACAGAGGGCACTAACTCAATCACAAAATTTTTCTCAACAAAGGGAACCAAAAAAGAAGAGCTAAAGCCAAATGATACAAGTTCTCATGACTCATCTGTGAAGACTGATTTTCCAGAAAGCATAAAAAAAGAACCTGAATACACAGAGGACAGGGTGCAACCATCCTTGACTGTTAAATGTGAAGAAGATTCAAAAAGTAGTGTCTCTACATTCTCTCAAGAGGAGGCATCTAAGGGGCAGACGAAGCGGGACTATGAAGAGTTCTTAGCTGATTCTAAACCGGTTCTTAATAAAGCTGATAAGATTTCTAGTCCTAGTCCAGCAAAGAAGAAGGTCAACCTGAAGACTTCTCAAGATAAGCAACCTACACTATTTTCATACTTTGGGAAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRAP

Protein Analysis

368

Amino Acids

41.56

Weight (kDa)

8.57

Isoelectric Point (pI)

49.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRAP PF02586 1 - 223 8.1e-72 SOS response associated peptidase (SRAP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26470 AT2G26470
fragaria_vesca FvH4_2g17880 FvH4_2g17880 FvH4_2g17880
malus_domestica MD05G1163100.v1.1
prunus_persica Prupe.8G191400_v2.0.a1
pyrus_communis pycom05g15260
rosa_chinensis RchiOBHm_Chr6g0283131
rosa_laevigata RLG00000012842
rosa_roxburghii Rroxscaffold_7G00185750
rosa_rugosa Rorug06G0151500.1 Rorug06G0151600
rosa_samantha Rh6AG264400 Rh6BG267300 Rh6CG266300 Rh6DG259600
rosa_wichuraiana Rw6G022880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 639
AccB1I GGYRCC 2 cut(s) 68, 639
AccBSI CCGCTC 1 cut(s) 128
AccII CGCG 1 cut(s) 16
AciI CCGC 4 cut(s) 58, 128, 322, 958
AcoI YGGCCR 1 cut(s) 101
AcsI RAATTY 2 cut(s) 448, 737
AcuI CTGAAG 3 cut(s) 474, 486, 1073
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 3 cut(s) 22, 79, 641
AfiI CCNNNNNNNGG 3 cut(s) 400, 620, 659
AgeI ACCGGT 2 cut(s) 93, 991
AgsI TTSAA 3 cut(s) 262, 709, 903
AluBI AGCT 5 cut(s) 506, 772, 980, 1007, 1104
AluI AGCT 5 cut(s) 506, 772, 980, 1007, 1104
Alw26I GTCTC 1 cut(s) 919
AoxI GGCC 3 cut(s) 101, 410, 680
ApeKI GCWGC 3 cut(s) 324, 425, 690
ApoI RAATTY 2 cut(s) 448, 737
AsiGI ACCGGT 2 cut(s) 93, 991
Asp718I GGTACC 1 cut(s) 639
AspLEI GCGC 3 cut(s) 16, 18, 71
AspS9I GGNCC 2 cut(s) 91, 680
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 3 cut(s) 182, 470, 557
AvaII GGWCC 1 cut(s) 91
BaeGI GKGCMC 1 cut(s) 722
BanI GGYRCC 2 cut(s) 68, 639
BbsI GAAGAC 4 cut(s) 180, 716, 818, 1061
BbvI GCAGC 3 cut(s) 311, 412, 702
BccI CCATC 5 cut(s) 149, 161, 359, 398, 880
BceAI ACGGC 3 cut(s) 56, 88, 193
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 919
BfaI CTAG 6 cut(s) 45, 308, 329, 1020, 1026, 1105
BfmI CTRYAG 1 cut(s) 336
BfoI RGCGCY 1 cut(s) 72
BisI GCNGC 3 cut(s) 325, 426, 691
BlsI GCNGC 3 cut(s) 326, 427, 692
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 2 cut(s) 91, 680
BmiI GGNNCC 4 cut(s) 70, 518, 641, 757
BmrFI CCNGG 1 cut(s) 123
BmrI ACTGGG 1 cut(s) 638
BmsI GCATC 2 cut(s) 519, 947
BmuI ACTGGG 1 cut(s) 638
BpiI GAAGAC 4 cut(s) 180, 716, 818, 1061
BpuEI CTTGAG 2 cut(s) 912, 1047
BpuMI CCSGG 1 cut(s) 123
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 64
BsaWI WCCGGW 3 cut(s) 93, 136, 991
Bsc4I CCNNNNNNNGG 3 cut(s) 400, 620, 659
Bse118I RCCGGY 2 cut(s) 93, 991
Bse1I ACTGG 2 cut(s) 533, 644
Bse3DI GCAATG 3 cut(s) 190, 663, 700
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 3 cut(s) 409, 534, 872
BseLI CCNNNNNNNGG 3 cut(s) 400, 620, 659
BseMI GCAATG 3 cut(s) 190, 663, 700
BseMII CTCAG 2 cut(s) 40, 264
BseNI ACTGG 2 cut(s) 533, 644
BsePI GCGCGC 1 cut(s) 14
BseRI GAGGAG 2 cut(s) 165, 947
BseSI GKGCMC 1 cut(s) 722
BseX3I CGGCCG 1 cut(s) 101
BseXI GCAGC 3 cut(s) 311, 412, 702
BsgI GTGCAG 1 cut(s) 709
Bsh1236I CGCG 1 cut(s) 16
Bsh1285I CGRYCG 2 cut(s) 104, 141
BshFI GGCC 3 cut(s) 103, 412, 682
BshNI GGYRCC 2 cut(s) 68, 639
BshTI ACCGGT 2 cut(s) 93, 991
BsiEI CGRYCG 2 cut(s) 104, 141
BsiSI CCGG 4 cut(s) 94, 122, 137, 992
BsiWI CGTACG 1 cut(s) 77
BslFI GGGAC 2 cut(s) 219, 974
BslI CCNNNNNNNGG 3 cut(s) 400, 620, 659
BsmAI GTCTC 1 cut(s) 919
BsmFI GGGAC 2 cut(s) 219, 974
BsnI GGCC 3 cut(s) 103, 412, 682
Bsp1286I GDGCHC 1 cut(s) 722
Bsp143I GATC 1 cut(s) 244
Bsp19I CCATGG 1 cut(s) 64
BspACI CCGC 4 cut(s) 58, 128, 322, 958
BspANI GGCC 3 cut(s) 103, 412, 682
BspCNI CTCAG 2 cut(s) 39, 265
BspFNI CGCG 1 cut(s) 16
BspHI TCATGA 1 cut(s) 796
BspLI GGNNCC 4 cut(s) 70, 518, 641, 757
BspQI GCTCTTC 1 cut(s) 762
BspT107I GGYRCC 2 cut(s) 68, 639
BsrBI CCGCTC 1 cut(s) 128
BsrDI GCAATG 3 cut(s) 190, 663, 700
BsrFI RCCGGY 2 cut(s) 93, 991
BsrI ACTGG 2 cut(s) 533, 644
BssAI RCCGGY 2 cut(s) 93, 991
BssECI CCNNGG 1 cut(s) 64
BssHII GCGCGC 1 cut(s) 14
BssMI GATC 1 cut(s) 244
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 64
Bst4CI ACNGT 2 cut(s) 705, 883
Bst6I CTCTTC 3 cut(s) 420, 762, 963
BstACI GRCGYC 1 cut(s) 69
BstC8I GCNNGC 3 cut(s) 16, 52, 268
BstDEI CTNAG 5 cut(s) 26, 273, 372, 942, 976
BstDSI CCRYGG 1 cut(s) 64
BstF5I GGATG 3 cut(s) 409, 534, 872
BstFNI CGCG 1 cut(s) 16
BstH2I RGCGCY 1 cut(s) 72
BstHHI GCGC 3 cut(s) 16, 18, 71
BstKTI GATC 1 cut(s) 247
BstMAI GTCTC 1 cut(s) 919
BstMBI GATC 1 cut(s) 244
BstMCI CGRYCG 2 cut(s) 104, 141
BstMWI GCNNNNNNNGC 2 cut(s) 66, 955
BstSCI CCNGG 1 cut(s) 121
BstSFI CTRYAG 1 cut(s) 336
BstSLI GKGCMC 1 cut(s) 722
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 3 cut(s) 311, 412, 702
BstV2I GAAGAC 4 cut(s) 180, 716, 818, 1061
BstZI CGGCCG 1 cut(s) 101
BsuRI GGCC 3 cut(s) 103, 412, 682
BtgI CCRYGG 1 cut(s) 64
BtsCI GGATG 3 cut(s) 409, 534, 872
Cac8I GCNNGC 3 cut(s) 16, 52, 268
CciI TCATGA 1 cut(s) 796
CfoI GCGC 3 cut(s) 16, 18, 71
Cfr10I RCCGGY 2 cut(s) 93, 991
Cfr13I GGNCC 2 cut(s) 91, 680
Csp6I GTAC 3 cut(s) 21, 78, 640
CspAI ACCGGT 2 cut(s) 93, 991
CviAII CATG 6 cut(s) 65, 88, 196, 521, 567, 797
CviQI GTAC 3 cut(s) 21, 78, 640
DdeI CTNAG 5 cut(s) 26, 273, 372, 942, 976
DinI GGCGCC 1 cut(s) 70
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 101
EagI CGGCCG 1 cut(s) 101
Eam1104I CTCTTC 3 cut(s) 420, 762, 963
EarI CTCTTC 3 cut(s) 420, 762, 963
EclXI CGGCCG 1 cut(s) 101
Eco130I CCWWGG 1 cut(s) 64
Eco47I GGWCC 1 cut(s) 91
Eco52I CGGCCG 1 cut(s) 101
Eco57I CTGAAG 3 cut(s) 474, 486, 1073
EcoRI GAATTC 1 cut(s) 448
EcoT14I CCWWGG 1 cut(s) 64
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 64
FaeI CATG 6 cut(s) 68, 91, 199, 524, 570, 800
FaqI GGGAC 2 cut(s) 219, 974
FatI CATG 6 cut(s) 64, 87, 195, 520, 566, 796
FauI CCCGC 1 cut(s) 951
FauNDI CATATG 1 cut(s) 616
Fnu4HI GCNGC 3 cut(s) 325, 426, 691
FokI GGATG 3 cut(s) 416, 541, 859
Fsp4HI GCNGC 3 cut(s) 325, 426, 691
FspBI CTAG 6 cut(s) 45, 308, 329, 1020, 1026, 1105
GlaI GCGC 3 cut(s) 15, 17, 70
GluI GCNGC 3 cut(s) 325, 426, 691
HaeII RGCGCY 1 cut(s) 72
HaeIII GGCC 3 cut(s) 103, 412, 682
HapII CCGG 4 cut(s) 94, 122, 137, 992
HhaI GCGC 3 cut(s) 16, 18, 71
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 6 cut(s) 68, 91, 199, 524, 570, 800
Hin6I GCGC 3 cut(s) 14, 16, 69
HinP1I GCGC 3 cut(s) 14, 16, 69
HincII GTYRAC 1 cut(s) 1048
HindII GTYRAC 1 cut(s) 1048
HinfI GANTC 8 cut(s) 211, 275, 437, 554, 578, 800, 899, 983
HpaII CCGG 4 cut(s) 94, 122, 137, 992
HphI GGTGA 3 cut(s) 182, 470, 557
Hpy166II GTNNAC 1 cut(s) 1048
Hpy188I TCNGA 6 cut(s) 29, 166, 216, 274, 454, 628
Hpy188III TCNNGA 4 cut(s) 797, 824, 929, 1064
Hpy8I GTNNAC 1 cut(s) 1048
Hpy99I CGWCG 4 cut(s) 38, 41, 109, 306
HpyAV CCTTC 5 cut(s) 335, 449, 484, 614, 1036
HpyCH4III ACNGT 2 cut(s) 705, 883
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 3 cut(s) 195, 690, 868
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 955
HpyF3I CTNAG 5 cut(s) 26, 273, 372, 942, 976
HpySE526I ACGT 1 cut(s) 39
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 6 cut(s) 68, 91, 199, 524, 570, 800
HspAI GCGC 3 cut(s) 14, 16, 69
KasI GGCGCC 1 cut(s) 68
KpnI GGTACC 1 cut(s) 643
Kzo9I GATC 1 cut(s) 244
LguI GCTCTTC 1 cut(s) 762
LmnI GCTCC 2 cut(s) 125, 522
Lsp1109I GCAGC 3 cut(s) 311, 412, 702
LweI GCATC 2 cut(s) 519, 947
MaeI CTAG 6 cut(s) 45, 308, 329, 1020, 1026, 1105
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 3 cut(s) 545, 603, 646
MalI GATC 1 cut(s) 246
MbiI CCGCTC 1 cut(s) 128
MboI GATC 1 cut(s) 244
MhlI GDGCHC 1 cut(s) 722
MluCI AATT 3 cut(s) 448, 592, 737
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 2 cut(s) 284, 794
MnlI CCTC 7 cut(s) 3, 143, 423, 709, 850, 925, 928
MseI TTAA 7 cut(s) 84, 399, 573, 609, 675, 885, 999
MspA1I CMGCKG 1 cut(s) 324
MspI CCGG 4 cut(s) 94, 122, 137, 992
MspR9I CCNGG 1 cut(s) 123
MvnI CGCG 1 cut(s) 16
MwoI GCNNNNNNNGC 2 cut(s) 66, 955
NarI GGCGCC 1 cut(s) 69
NciI CCSGG 1 cut(s) 123
NcoI CCATGG 1 cut(s) 64
NdeI CATATG 1 cut(s) 616
NdeII GATC 1 cut(s) 244
NlaIII CATG 6 cut(s) 68, 91, 199, 524, 570, 800
NlaIV GGNNCC 4 cut(s) 70, 518, 641, 757
NmuCI GTSAC 1 cut(s) 545
PagI TCATGA 1 cut(s) 796
PauI GCGCGC 1 cut(s) 14
PciSI GCTCTTC 1 cut(s) 762
PfeI GAWTC 6 cut(s) 211, 437, 554, 578, 899, 983
Pfl23II CGTACG 1 cut(s) 77
PinAI ACCGGT 2 cut(s) 93, 991
PkrI GCNGC 3 cut(s) 326, 427, 692
PleI GAGTC 2 cut(s) 283, 794
PluTI GGCGCC 1 cut(s) 72
PpsI GAGTC 2 cut(s) 283, 794
PspLI CGTACG 1 cut(s) 77
PspN4I GGNNCC 4 cut(s) 70, 518, 641, 757
PspPI GGNCC 2 cut(s) 91, 680
PteI GCGCGC 1 cut(s) 14
RsaI GTAC 3 cut(s) 22, 79, 641
RsaNI GTAC 3 cut(s) 21, 78, 640
SapI GCTCTTC 1 cut(s) 762
SaqAI TTAA 7 cut(s) 84, 399, 573, 609, 675, 885, 999
SatI GCNGC 3 cut(s) 325, 426, 691
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 2 cut(s) 91, 680
SchI GAGTC 2 cut(s) 284, 794
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 1 cut(s) 722
SfaNI GCATC 2 cut(s) 519, 947
SfcI CTRYAG 1 cut(s) 336
SfoI GGCGCC 1 cut(s) 70
SinI GGWCC 1 cut(s) 91
SmlI CTYRAG 2 cut(s) 927, 1062
SmoI CTYRAG 2 cut(s) 927, 1062
Sse9I AATT 3 cut(s) 448, 592, 737
SsiI CCGC 4 cut(s) 58, 128, 322, 958
SspDI GGCGCC 1 cut(s) 68
SspMI CTAG 6 cut(s) 45, 308, 329, 1020, 1026, 1105
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 1 cut(s) 64
TaaI ACNGT 2 cut(s) 705, 883
TaiI ACGT 1 cut(s) 42
TasI AATT 3 cut(s) 448, 592, 737
TatI WGTACW 1 cut(s) 20
TfiI GAWTC 6 cut(s) 211, 437, 554, 578, 899, 983
Tru1I TTAA 7 cut(s) 84, 399, 573, 609, 675, 885, 999
Tru9I TTAA 7 cut(s) 84, 399, 573, 609, 675, 885, 999
TseFI GTSAC 1 cut(s) 545
TseI GCWGC 3 cut(s) 324, 425, 690
Tsp45I GTSAC 1 cut(s) 545
TspDTI ATGAA 8 cut(s) 179, 212, 366, 486, 573, 633, 981, 1077
TspGWI ACGGA 1 cut(s) 248
VpaK11BI GGWCC 1 cut(s) 91
XapI RAATTY 2 cut(s) 448, 737
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XspI CTAG 6 cut(s) 45, 308, 329, 1020, 1026, 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.