Rh6AG264400

Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0013250
Physical Location & Seq
Forward (+)
45588375 .. 45591869
3495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG264400.1

Sequence Viewer

Length: 801 bp
ATGGTGTGGCGTTTCGGTTCCCGCCTTGTTGAGTTTTCTTCTTCCTTGGAGATGTGTGGGAGGTCGCGCTGTACTCTCAGAGCCGACGACGTTTCTAGGGCTTGCTACCGCAACCATGGCGCCGTTCGTACGATTAACATGGACCGGTATCGGCCGTCGTATAATGTGTCGCCGGGAGCGGATTTGCCGGTCGTGCGTAGAGGAGATGGTGGGTCAGATGGTGAAGACGGCGTTGTTCTTCATTGCATGAAATGGGGACTGATTCCGAGTTTCTCCAAGAAAACGGAGAAGCCCGATCATTACAGGATGTTCAATGCTCGCTCTGAGTCCATTTGTGAAAAGGCTTCTTTTCGTCGTCTAGTTCCTAAAAACCGCTGCCTAGTTGCTGTAGAAGGATTTTATGAATGGAAAAAAGATGGTTCTAAGAAACAGCCTTACTATGTCCATTTTAAGGATGGTCGGCCTCTTCTGTTTGCTGCTCTTTATGATTCGTGGGAGAACTCTGAAGGTGAGAAACTTTATACCTTCACTATCATTACAACTTCTTCATCTTCAGCTTTGGCGTGGCTCCATGACAGGATGCCAGTTATTTTGGGTGACAAGGAATCTATTGATACATGGCTTAATGATTCTTCATCTTCCAATTTTGACAAGTTACTTAAACCATATGAAGGGTCTGATTTGGTATGGTACCCAGTAACACCAGCAATGGGTAAGGTGTCTTTTAATGGGCCAGAGTGCAGCAATGAGGTGCGACAGTTACTGTGTTTTCTTTTACACATGATGTGCATCATTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRAP

Protein Analysis

266

Amino Acids

30.45

Weight (kDa)

8.51

Isoelectric Point (pI)

46.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRAP PF02586 18 - 240 3.1e-72 SOS response associated peptidase (SRAP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26470 AT2G26470
fragaria_vesca FvH4_2g17880 FvH4_2g17880 FvH4_2g17880
malus_domestica MD05G1163100.v1.1
prunus_persica Prupe.8G191400_v2.0.a1
pyrus_communis pycom05g15260
rosa_chinensis RchiOBHm_Chr6g0283131
rosa_laevigata RLG00000012842
rosa_roxburghii Rroxscaffold_7G00185750
rosa_rugosa Rorug06G0151500.1 Rorug06G0151600
rosa_samantha Rh6AG264400 Rh6BG267300 Rh6CG266300 Rh6DG259600
rosa_wichuraiana Rw6G022880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 690
AccB1I GGYRCC 2 cut(s) 119, 690
AccBSI CCGCTC 1 cut(s) 179
AccII CGCG 1 cut(s) 67
AciI CCGC 4 cut(s) 22, 109, 179, 373
AcoI YGGCCR 1 cut(s) 152
AcuI CTGAAG 2 cut(s) 525, 537
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 3 cut(s) 73, 130, 692
AfiI CCNNNNNNNGG 3 cut(s) 451, 671, 710
AgeI ACCGGT 1 cut(s) 144
AgsI TTSAA 1 cut(s) 313
AluBI AGCT 1 cut(s) 557
AluI AGCT 1 cut(s) 557
AlwNI CAGNNNCTG 1 cut(s) 763
AoxI GGCC 3 cut(s) 152, 461, 731
ApeKI GCWGC 3 cut(s) 375, 476, 741
AsiGI ACCGGT 1 cut(s) 144
Asp718I GGTACC 1 cut(s) 690
AspLEI GCGC 2 cut(s) 69, 122
AspS9I GGNCC 2 cut(s) 142, 731
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 3 cut(s) 233, 521, 608
AvaII GGWCC 1 cut(s) 142
BanI GGYRCC 2 cut(s) 119, 690
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 3 cut(s) 362, 463, 753
BccI CCATC 4 cut(s) 200, 212, 410, 449
BceAI ACGGC 3 cut(s) 107, 139, 244
BcnI CCSGG 1 cut(s) 174
BfaI CTAG 3 cut(s) 96, 359, 380
BfmI CTRYAG 1 cut(s) 387
BfoI RGCGCY 1 cut(s) 123
BisI GCNGC 3 cut(s) 376, 477, 742
BlsI GCNGC 3 cut(s) 377, 478, 743
Bme1390I CCNGG 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 142
BmgT120I GGNCC 2 cut(s) 142, 731
BmiI GGNNCC 4 cut(s) 19, 121, 569, 692
BmrFI CCNGG 1 cut(s) 174
BmrI ACTGGG 1 cut(s) 689
BmsI GCATC 1 cut(s) 570
BmuI ACTGGG 1 cut(s) 689
BpiI GAAGAC 1 cut(s) 231
BpuMI CCSGG 1 cut(s) 174
BsaBI GATNNNNATC 1 cut(s) 788
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 2 cut(s) 45, 115
BsaWI WCCGGW 1 cut(s) 144
Bsc4I CCNNNNNNNGG 3 cut(s) 451, 671, 710
Bse118I RCCGGY 2 cut(s) 144, 187
Bse1I ACTGG 2 cut(s) 584, 695
Bse3DI GCAATG 3 cut(s) 241, 714, 751
Bse8I GATNNNNATC 1 cut(s) 788
BseDI CCNNGG 2 cut(s) 45, 115
BseGI GGATG 3 cut(s) 312, 460, 585
BseJI GATNNNNATC 1 cut(s) 788
BseLI CCNNNNNNNGG 3 cut(s) 451, 671, 710
BseMI GCAATG 3 cut(s) 241, 714, 751
BseMII CTCAG 2 cut(s) 91, 315
BseNI ACTGG 2 cut(s) 584, 695
BseRI GAGGAG 1 cut(s) 216
BseX3I CGGCCG 1 cut(s) 152
BseXI GCAGC 3 cut(s) 362, 463, 753
BsgI GTGCAG 1 cut(s) 760
Bsh1236I CGCG 1 cut(s) 67
Bsh1285I CGRYCG 2 cut(s) 155, 192
BshFI GGCC 3 cut(s) 154, 463, 733
BshNI GGYRCC 2 cut(s) 119, 690
BshTI ACCGGT 1 cut(s) 144
BsiEI CGRYCG 2 cut(s) 155, 192
BsiSI CCGG 3 cut(s) 145, 173, 188
BsiWI CGTACG 1 cut(s) 128
BslFI GGGAC 1 cut(s) 270
BslI CCNNNNNNNGG 3 cut(s) 451, 671, 710
BsmFI GGGAC 1 cut(s) 270
BsnI GGCC 3 cut(s) 154, 463, 733
Bsp143I GATC 1 cut(s) 295
Bsp19I CCATGG 1 cut(s) 115
BspACI CCGC 4 cut(s) 22, 109, 179, 373
BspANI GGCC 3 cut(s) 154, 463, 733
BspCNI CTCAG 2 cut(s) 90, 316
BspFNI CGCG 1 cut(s) 67
BspLI GGNNCC 4 cut(s) 19, 121, 569, 692
BspT107I GGYRCC 2 cut(s) 119, 690
BsrBI CCGCTC 1 cut(s) 179
BsrDI GCAATG 3 cut(s) 241, 714, 751
BsrFI RCCGGY 2 cut(s) 144, 187
BsrI ACTGG 2 cut(s) 584, 695
BssAI RCCGGY 2 cut(s) 144, 187
BssECI CCNNGG 2 cut(s) 45, 115
BssMI GATC 1 cut(s) 295
BssNI GRCGYC 1 cut(s) 120
BssT1I CCWWGG 2 cut(s) 45, 115
Bst4CI ACNGT 2 cut(s) 759, 765
Bst6I CTCTTC 1 cut(s) 471
BstACI GRCGYC 1 cut(s) 120
BstC8I GCNNGC 2 cut(s) 103, 319
BstDEI CTNAG 3 cut(s) 77, 324, 423
BstDSI CCRYGG 1 cut(s) 115
BstF5I GGATG 3 cut(s) 312, 460, 585
BstFNI CGCG 1 cut(s) 67
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 2 cut(s) 69, 122
BstKTI GATC 1 cut(s) 298
BstMBI GATC 1 cut(s) 295
BstMCI CGRYCG 2 cut(s) 155, 192
BstMWI GCNNNNNNNGC 2 cut(s) 117, 193
BstSCI CCNGG 1 cut(s) 172
BstSFI CTRYAG 1 cut(s) 387
BstUI CGCG 1 cut(s) 67
BstV1I GCAGC 3 cut(s) 362, 463, 753
BstV2I GAAGAC 1 cut(s) 231
BstZI CGGCCG 1 cut(s) 152
BsuRI GGCC 3 cut(s) 154, 463, 733
BtgI CCRYGG 1 cut(s) 115
BtsCI GGATG 3 cut(s) 312, 460, 585
Cac8I GCNNGC 2 cut(s) 103, 319
CaiI CAGNNNCTG 1 cut(s) 763
CfoI GCGC 2 cut(s) 69, 122
Cfr10I RCCGGY 2 cut(s) 144, 187
Cfr13I GGNCC 2 cut(s) 142, 731
Csp6I GTAC 3 cut(s) 72, 129, 691
CspAI ACCGGT 1 cut(s) 144
CviAII CATG 6 cut(s) 116, 139, 247, 572, 618, 781
CviQI GTAC 3 cut(s) 72, 129, 691
DdeI CTNAG 3 cut(s) 77, 324, 423
DinI GGCGCC 1 cut(s) 121
DpnI GATC 1 cut(s) 297
DpnII GATC 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 152
EagI CGGCCG 1 cut(s) 152
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
EclXI CGGCCG 1 cut(s) 152
Eco130I CCWWGG 2 cut(s) 45, 115
Eco47I GGWCC 1 cut(s) 142
Eco52I CGGCCG 1 cut(s) 152
Eco57I CTGAAG 2 cut(s) 525, 537
EcoT14I CCWWGG 2 cut(s) 45, 115
EgeI GGCGCC 1 cut(s) 121
EheI GGCGCC 1 cut(s) 121
ErhI CCWWGG 2 cut(s) 45, 115
FaeI CATG 6 cut(s) 119, 142, 250, 575, 621, 784
FaqI GGGAC 1 cut(s) 270
FatI CATG 6 cut(s) 115, 138, 246, 571, 617, 780
FauI CCCGC 1 cut(s) 29
FauNDI CATATG 1 cut(s) 667
Fnu4HI GCNGC 3 cut(s) 376, 477, 742
FokI GGATG 3 cut(s) 319, 467, 592
Fsp4HI GCNGC 3 cut(s) 376, 477, 742
FspBI CTAG 3 cut(s) 96, 359, 380
GlaI GCGC 2 cut(s) 68, 121
GluI GCNGC 3 cut(s) 376, 477, 742
HaeII RGCGCY 1 cut(s) 123
HaeIII GGCC 3 cut(s) 154, 463, 733
HapII CCGG 3 cut(s) 145, 173, 188
HhaI GCGC 2 cut(s) 69, 122
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 6 cut(s) 119, 142, 250, 575, 621, 784
Hin6I GCGC 2 cut(s) 67, 120
HinP1I GCGC 2 cut(s) 67, 120
HinfI GANTC 5 cut(s) 262, 326, 488, 605, 629
HpaII CCGG 3 cut(s) 145, 173, 188
HphI GGTGA 3 cut(s) 233, 521, 608
Hpy188I TCNGA 6 cut(s) 80, 217, 267, 325, 505, 679
Hpy99I CGWCG 4 cut(s) 89, 92, 160, 357
HpyAV CCTTC 4 cut(s) 386, 500, 535, 665
HpyCH4III ACNGT 2 cut(s) 759, 765
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 3 cut(s) 246, 741, 789
HpyF10VI GCNNNNNNNGC 2 cut(s) 117, 193
HpyF3I CTNAG 3 cut(s) 77, 324, 423
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 6 cut(s) 119, 142, 250, 575, 621, 784
HspAI GCGC 2 cut(s) 67, 120
KasI GGCGCC 1 cut(s) 119
KpnI GGTACC 1 cut(s) 694
Kzo9I GATC 1 cut(s) 295
LmnI GCTCC 2 cut(s) 176, 573
LpnPI CCDG 9 cut(s) 158, 186, 201, 289, 562, 597, 708, 717, 747
Lsp1109I GCAGC 3 cut(s) 362, 463, 753
LweI GCATC 1 cut(s) 570
MaeI CTAG 3 cut(s) 96, 359, 380
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 4 cut(s) 596, 654, 697, 759
MalI GATC 1 cut(s) 297
MbiI CCGCTC 1 cut(s) 179
MboI GATC 1 cut(s) 295
MboII GAAGA 9 cut(s) 30, 33, 230, 236, 458, 537, 543, 624, 630
MluCI AATT 1 cut(s) 643
Mly113I GGCGCC 1 cut(s) 120
MlyI GAGTC 1 cut(s) 335
MnlI CCTC 4 cut(s) 54, 194, 474, 742
MseI TTAA 5 cut(s) 135, 450, 624, 660, 726
MspA1I CMGCKG 1 cut(s) 375
MspI CCGG 3 cut(s) 145, 173, 188
MspR9I CCNGG 1 cut(s) 174
MvnI CGCG 1 cut(s) 67
MwoI GCNNNNNNNGC 2 cut(s) 117, 193
NarI GGCGCC 1 cut(s) 120
NciI CCSGG 1 cut(s) 174
NcoI CCATGG 1 cut(s) 115
NdeI CATATG 1 cut(s) 667
NdeII GATC 1 cut(s) 295
NlaIII CATG 6 cut(s) 119, 142, 250, 575, 621, 784
NlaIV GGNNCC 4 cut(s) 19, 121, 569, 692
NmuCI GTSAC 1 cut(s) 596
PfeI GAWTC 4 cut(s) 262, 488, 605, 629
Pfl23II CGTACG 1 cut(s) 128
PinAI ACCGGT 1 cut(s) 144
PkrI GCNGC 3 cut(s) 377, 478, 743
PleI GAGTC 1 cut(s) 334
PluTI GGCGCC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 334
PspLI CGTACG 1 cut(s) 128
PspN4I GGNNCC 4 cut(s) 19, 121, 569, 692
PspPI GGNCC 2 cut(s) 142, 731
PstNI CAGNNNCTG 1 cut(s) 763
RsaI GTAC 3 cut(s) 73, 130, 692
RsaNI GTAC 3 cut(s) 72, 129, 691
SaqAI TTAA 5 cut(s) 135, 450, 624, 660, 726
SatI GCNGC 3 cut(s) 376, 477, 742
Sau3AI GATC 1 cut(s) 295
Sau96I GGNCC 2 cut(s) 142, 731
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 1 cut(s) 174
SetI ASST 7 cut(s) 65, 93, 511, 527, 559, 720, 753
SfaNI GCATC 1 cut(s) 570
SfcI CTRYAG 1 cut(s) 387
SfoI GGCGCC 1 cut(s) 121
SinI GGWCC 1 cut(s) 142
Sse9I AATT 1 cut(s) 643
SsiI CCGC 4 cut(s) 22, 109, 179, 373
SspDI GGCGCC 1 cut(s) 119
SspMI CTAG 3 cut(s) 96, 359, 380
StyD4I CCNGG 1 cut(s) 172
StyI CCWWGG 2 cut(s) 45, 115
TaaI ACNGT 2 cut(s) 759, 765
TaiI ACGT 1 cut(s) 93
TasI AATT 1 cut(s) 643
TatI WGTACW 1 cut(s) 71
TfiI GAWTC 4 cut(s) 262, 488, 605, 629
Tru1I TTAA 5 cut(s) 135, 450, 624, 660, 726
Tru9I TTAA 5 cut(s) 135, 450, 624, 660, 726
TseFI GTSAC 1 cut(s) 596
TseI GCWGC 3 cut(s) 375, 476, 741
Tsp45I GTSAC 1 cut(s) 596
TspDTI ATGAA 6 cut(s) 230, 263, 417, 537, 624, 684
TspGWI ACGGA 1 cut(s) 299
VpaK11BI GGWCC 1 cut(s) 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 452
XspI CTAG 3 cut(s) 96, 359, 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.