Rh6CG266300

Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0013250
Physical Location & Seq
Forward (+)
43983428 .. 43986922
3495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG266300.1

Sequence Viewer

Length: 885 bp
ATGGTTGTGATTTATGATTTGTGTAAACAACTGCAGTTCAATGCTCGCTCTGAGTCCATTTGTGAAAAGGCTTCTTTTCGTCGTCTAGTTCCTAAAAACCGCTGCCTAGTTGCTGTAGAAGGATTTTATGAATGGAAAAAAGATGGTTCTAAGAAACAGCCTTACTATGTCCATTTTAAGGATGGTCGGCCTCTTCTGTTTGCTGCTCTTTATGATTCGTGGGAGAACTCTGAAGGTGAGAAACTTTATACCTTCACTATCATTACAACTTCTTCATCTTCAGCTTTGGCGTGGCTCCATGACAGGATGCCAGTTATTTTGGGTGACAAGGAATCTATTGATACATGGCTTAATGATTCTTCATCTTCCAATTTTGACAAGTTACTTAAACCATATGAAGGGTCTGATTTGGTATGGTACCCAGTAACACCAGCAATGGGTAAGGTGTCTTTTAATGGGCCAGAGTGCAGCAATGAGATACAGTTGAAGACAGAGGGCACTAACTCAATCACAAAATTTTTCTCAACAAAGGGAACCAAAAAAGAAGAGCTAAAGCCAAATGATACAAGTTCTCATGACTCATCTGTGAAGACTGATTTTCCAGAAAGCATAAAAAAAGAACCTGAATACACAGAGGACAGGGTGCAACCATCCTTGACTGTGAAATGTGAAGAAGATTCAAAAAGTAGTGTCTCTACATTCTCTCAAGAGGAGGCATCTAAGGGGCAGACGAAGCGGAACTATGAAGAGTTCTTAGCTGATTCTAAACCGGTTCTTAATAAAGCTGATAAGATTTCTAGTGCTAGTCCAGCAAAGAAGAAGGTCAACCTGAAGACTTCTCAAGATAAGCAACCTACACTATTTTCTTACTTTGGGAAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRAP

Protein Analysis

294

Amino Acids

33.29

Weight (kDa)

7.54

Isoelectric Point (pI)

49.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRAP PF02586 11 - 149 4e-52 SOS response associated peptidase (SRAP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26470 AT2G26470
fragaria_vesca FvH4_2g17880 FvH4_2g17880 FvH4_2g17880
malus_domestica MD05G1163100.v1.1
prunus_persica Prupe.8G191400_v2.0.a1
pyrus_communis pycom05g15260
rosa_chinensis RchiOBHm_Chr6g0283131
rosa_laevigata RLG00000012842
rosa_roxburghii Rroxscaffold_7G00185750
rosa_rugosa Rorug06G0151500.1 Rorug06G0151600
rosa_samantha Rh6AG264400 Rh6BG267300 Rh6CG266300 Rh6DG259600
rosa_wichuraiana Rw6G022880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 417
AccB1I GGYRCC 1 cut(s) 417
AciI CCGC 2 cut(s) 100, 736
AcsI RAATTY 1 cut(s) 515
AcuI CTGAAG 3 cut(s) 252, 264, 851
AfaI GTAC 1 cut(s) 419
AfiI CCNNNNNNNGG 3 cut(s) 178, 398, 437
AgeI ACCGGT 1 cut(s) 769
AgsI TTSAA 3 cut(s) 40, 487, 681
AluBI AGCT 5 cut(s) 284, 550, 758, 785, 882
AluI AGCT 5 cut(s) 284, 550, 758, 785, 882
Alw26I GTCTC 1 cut(s) 697
AoxI GGCC 2 cut(s) 188, 458
ApeKI GCWGC 3 cut(s) 102, 203, 468
ApoI RAATTY 1 cut(s) 515
AsiGI ACCGGT 1 cut(s) 769
Asp718I GGTACC 1 cut(s) 417
AspS9I GGNCC 1 cut(s) 458
AsuHPI GGTGA 2 cut(s) 248, 335
BaeGI GKGCMC 1 cut(s) 500
BanI GGYRCC 1 cut(s) 417
BbsI GAAGAC 3 cut(s) 494, 596, 839
BbvI GCAGC 3 cut(s) 89, 190, 480
BccI CCATC 3 cut(s) 137, 176, 658
BcoDI GTCTC 1 cut(s) 697
BfaI CTAG 5 cut(s) 86, 107, 798, 804, 883
BfmI CTRYAG 2 cut(s) 32, 114
BisI GCNGC 3 cut(s) 103, 204, 469
BlsI GCNGC 3 cut(s) 104, 205, 470
BmgT120I GGNCC 1 cut(s) 458
BmiI GGNNCC 3 cut(s) 296, 419, 535
BmrI ACTGGG 1 cut(s) 416
BmsI GCATC 2 cut(s) 297, 725
BmuI ACTGGG 1 cut(s) 416
BpiI GAAGAC 3 cut(s) 494, 596, 839
BpuEI CTTGAG 2 cut(s) 690, 825
BsaWI WCCGGW 1 cut(s) 769
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 398, 437
Bse118I RCCGGY 1 cut(s) 769
Bse1I ACTGG 2 cut(s) 311, 422
Bse3DI GCAATG 2 cut(s) 441, 478
BseGI GGATG 3 cut(s) 187, 312, 650
BseLI CCNNNNNNNGG 3 cut(s) 178, 398, 437
BseMI GCAATG 2 cut(s) 441, 478
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 2 cut(s) 311, 422
BseRI GAGGAG 1 cut(s) 725
BseSI GKGCMC 1 cut(s) 500
BseXI GCAGC 3 cut(s) 89, 190, 480
BsgI GTGCAG 1 cut(s) 487
BshFI GGCC 2 cut(s) 190, 460
BshNI GGYRCC 1 cut(s) 417
BshTI ACCGGT 1 cut(s) 769
BsiSI CCGG 1 cut(s) 770
BslI CCNNNNNNNGG 3 cut(s) 178, 398, 437
BsmAI GTCTC 1 cut(s) 697
BsnI GGCC 2 cut(s) 190, 460
Bsp1286I GDGCHC 1 cut(s) 500
BspACI CCGC 2 cut(s) 100, 736
BspANI GGCC 2 cut(s) 190, 460
BspCNI CTCAG 1 cut(s) 43
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 3 cut(s) 296, 419, 535
BspMAI CTGCAG 1 cut(s) 36
BspQI GCTCTTC 1 cut(s) 540
BspT107I GGYRCC 1 cut(s) 417
BsrDI GCAATG 2 cut(s) 441, 478
BsrFI RCCGGY 1 cut(s) 769
BsrI ACTGG 2 cut(s) 311, 422
BssAI RCCGGY 1 cut(s) 769
Bst4CI ACNGT 2 cut(s) 483, 661
Bst6I CTCTTC 3 cut(s) 198, 540, 741
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 4 cut(s) 51, 150, 720, 754
BstF5I GGATG 3 cut(s) 187, 312, 650
BstMAI GTCTC 1 cut(s) 697
BstMWI GCNNNNNNNGC 2 cut(s) 733, 809
BstSFI CTRYAG 2 cut(s) 32, 114
BstSLI GKGCMC 1 cut(s) 500
BstV1I GCAGC 3 cut(s) 89, 190, 480
BstV2I GAAGAC 3 cut(s) 494, 596, 839
BsuRI GGCC 2 cut(s) 190, 460
BtsCI GGATG 3 cut(s) 187, 312, 650
Cac8I GCNNGC 1 cut(s) 46
CciI TCATGA 1 cut(s) 574
Cfr10I RCCGGY 1 cut(s) 769
Cfr13I GGNCC 1 cut(s) 458
Csp6I GTAC 1 cut(s) 418
CspAI ACCGGT 1 cut(s) 769
CviAII CATG 3 cut(s) 299, 345, 575
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 4 cut(s) 51, 150, 720, 754
Eam1104I CTCTTC 3 cut(s) 198, 540, 741
EarI CTCTTC 3 cut(s) 198, 540, 741
Eco57I CTGAAG 3 cut(s) 252, 264, 851
FaeI CATG 3 cut(s) 302, 348, 578
FatI CATG 3 cut(s) 298, 344, 574
FauNDI CATATG 1 cut(s) 394
Fnu4HI GCNGC 3 cut(s) 103, 204, 469
FokI GGATG 3 cut(s) 194, 319, 637
Fsp4HI GCNGC 3 cut(s) 103, 204, 469
FspBI CTAG 5 cut(s) 86, 107, 798, 804, 883
GluI GCNGC 3 cut(s) 103, 204, 469
HaeIII GGCC 2 cut(s) 190, 460
HapII CCGG 1 cut(s) 770
Hin1II CATG 3 cut(s) 302, 348, 578
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HinfI GANTC 7 cut(s) 53, 215, 332, 356, 578, 677, 761
HpaII CCGG 1 cut(s) 770
HphI GGTGA 2 cut(s) 248, 335
Hpy166II GTNNAC 2 cut(s) 26, 826
Hpy188I TCNGA 3 cut(s) 52, 232, 406
Hpy188III TCNNGA 4 cut(s) 575, 602, 707, 842
Hpy8I GTNNAC 2 cut(s) 26, 826
Hpy99I CGWCG 1 cut(s) 84
HpyAV CCTTC 5 cut(s) 113, 227, 262, 392, 814
HpyCH4III ACNGT 2 cut(s) 483, 661
HpyCH4V TGCA 3 cut(s) 34, 468, 646
HpyF10VI GCNNNNNNNGC 2 cut(s) 733, 809
HpyF3I CTNAG 4 cut(s) 51, 150, 720, 754
Hsp92II CATG 3 cut(s) 302, 348, 578
KpnI GGTACC 1 cut(s) 421
LguI GCTCTTC 1 cut(s) 540
LmnI GCTCC 1 cut(s) 300
Lsp1109I GCAGC 3 cut(s) 89, 190, 480
LweI GCATC 2 cut(s) 297, 725
MaeI CTAG 5 cut(s) 86, 107, 798, 804, 883
MaeIII GTNAC 3 cut(s) 323, 381, 424
MhlI GDGCHC 1 cut(s) 500
MluCI AATT 2 cut(s) 370, 515
MlyI GAGTC 2 cut(s) 62, 572
MnlI CCTC 5 cut(s) 201, 487, 628, 703, 706
MseI TTAA 5 cut(s) 177, 351, 387, 453, 777
MspA1I CMGCKG 1 cut(s) 102
MspI CCGG 1 cut(s) 770
MwoI GCNNNNNNNGC 2 cut(s) 733, 809
NdeI CATATG 1 cut(s) 394
NlaIII CATG 3 cut(s) 302, 348, 578
NlaIV GGNNCC 3 cut(s) 296, 419, 535
NmuCI GTSAC 1 cut(s) 323
PagI TCATGA 1 cut(s) 574
PciSI GCTCTTC 1 cut(s) 540
PfeI GAWTC 5 cut(s) 215, 332, 356, 677, 761
PinAI ACCGGT 1 cut(s) 769
PkrI GCNGC 3 cut(s) 104, 205, 470
PleI GAGTC 2 cut(s) 61, 572
PpsI GAGTC 2 cut(s) 61, 572
PspN4I GGNNCC 3 cut(s) 296, 419, 535
PspPI GGNCC 1 cut(s) 458
PstI CTGCAG 1 cut(s) 36
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
SapI GCTCTTC 1 cut(s) 540
SaqAI TTAA 5 cut(s) 177, 351, 387, 453, 777
SatI GCNGC 3 cut(s) 103, 204, 469
Sau96I GGNCC 1 cut(s) 458
SchI GAGTC 2 cut(s) 62, 572
SduI GDGCHC 1 cut(s) 500
SfaNI GCATC 2 cut(s) 297, 725
SfcI CTRYAG 2 cut(s) 32, 114
SmlI CTYRAG 2 cut(s) 705, 840
SmoI CTYRAG 2 cut(s) 705, 840
Sse9I AATT 2 cut(s) 370, 515
SsiI CCGC 2 cut(s) 100, 736
SspMI CTAG 5 cut(s) 86, 107, 798, 804, 883
TaaI ACNGT 2 cut(s) 483, 661
TasI AATT 2 cut(s) 370, 515
TfiI GAWTC 5 cut(s) 215, 332, 356, 677, 761
Tru1I TTAA 5 cut(s) 177, 351, 387, 453, 777
Tru9I TTAA 5 cut(s) 177, 351, 387, 453, 777
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 3 cut(s) 102, 203, 468
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 5 cut(s) 144, 264, 351, 411, 759
XapI RAATTY 1 cut(s) 515
XcmI CCANNNNNNNNNTGG 1 cut(s) 179
XspI CTAG 5 cut(s) 86, 107, 798, 804, 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.