pycom05g15260

Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013250
Physical Location & Seq
Forward (+)
18766186 .. 18769608
3423 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g15260.5

Sequence Viewer

Length: 1182 bp
ATGGAAATTCTGTACGGGCAGAAAGTCCCACCGCATTCGCTCGCTTCCCGCCCTTTGTTCAGTTGCTTCGATTCTTGTATCGAGATGTGTGGAAGGGCTCGCTGTACTCTCAGAGCCGATGACGTCACTAGGGCTTGCCACCGCACCCACGCCCCCGTCCGCGCCGTTAACATGGACCGGTATCGTCCGTCGTACAATGTGTCGCCTGGATCGAATTTGCCTGTTGTTCGGAAAGAAGACGGGGCGGATGGCGACGGCGTCGTTCTTCAATGTATGAAATGGGGGCTGATTCCTAGTTTCACCAAGAAAACCGAAAAGCCCGATTTCTACAGGATGTTTAATGCTCGGTCCGAGTCCATTTGTGAAAAGGCTTCTTTTCGTCGTTTGGTTCCTAAAAGCCGCTGCATTGTTGCGGTTGAAGGTTTTTATGAATGGAAAAAAGATGGTTCTAAAAAGCAGCCTTACTACGTCCATTTCAAGGACGGCAGGCCTCTTCTGTTTGCTGCTCTTTATGATTCTTGGGAGAACTCTGAAGGAGAGAAACTTTACACATTCACTATCATAACAACTTCCTCATCATCAGCTTTAGGGTGGCTGCATGACAGGATGCCTGTTATCTTGGGTGATAAAGAATCAACTGACACATGGCTAGATGGTTCTTCATCGTCCAATTTTGATTCATTACTTAAACCATACGAAGGTCCAGATTTGGTATGGTACCCGGTGACACCAGCAATGGGTAAGGTGTCTTTTGATGGGCCAGAGTGCATCAATGAGATACAGTTGAAGACCGAGGGGAATAATTCAATCACAAAGTTTTTCTCAGCGAAGGGAACCAAAAAAGAAGAATTAAACCCAAAGGATACAAGTTCTGATGACACGTCTGCGAAGAAAGATTTGTCAAAAATGGTGAAAGAAGAACCTGAAAGCAAAGAGGACACAGAACAGCCGTACTCTACTGAACAATGTGAAGATGAATCAAAATGTAATGTGTCTACATTCTCTCAAGAAGGTGTATCTAAGGGCCAAGCAAAGCGAGACTACGAAGAATTTTCAGCTGATTCTAAGCCAGTTGCTTATGTAACGAATAAGAAATCTGCTAGTCTGGCAAAGAAGAAAGTTAACCCAAAGAGTTCTCTAGACAAGCAACCTACCCTATTTTCGTACTTTGGTAAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRAP

Protein Analysis

394

Amino Acids

44.19

Weight (kDa)

7.88

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26470 AT2G26470
fragaria_vesca FvH4_2g17880 FvH4_2g17880 FvH4_2g17880
malus_domestica MD05G1163100.v1.1
prunus_persica Prupe.8G191400_v2.0.a1
pyrus_communis pycom05g15260
rosa_chinensis RchiOBHm_Chr6g0283131
rosa_laevigata RLG00000012842
rosa_roxburghii Rroxscaffold_7G00185750
rosa_rugosa Rorug06G0151500.1 Rorug06G0151600
rosa_samantha Rh6AG264400 Rh6BG267300 Rh6CG266300 Rh6DG259600
rosa_wichuraiana Rw6G022880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 126
Acc65I GGTACC 1 cut(s) 717
AccB1I GGYRCC 1 cut(s) 717
AccI GTMKAC 1 cut(s) 995
AccII CGCG 1 cut(s) 162
AciI CCGC 7 cut(s) 32, 49, 142, 160, 245, 400, 413
AclWI GGATC 1 cut(s) 217
AcsI RAATTY 3 cut(s) 6, 214, 1049
AcuI CTGAAG 1 cut(s) 552
AcyI GRCGYC 2 cut(s) 123, 258
AfaI GTAC 6 cut(s) 14, 106, 194, 719, 953, 1166
AfiI CCNNNNNNNGG 3 cut(s) 478, 698, 737
AflIII ACRYGT 1 cut(s) 879
AgeI ACCGGT 1 cut(s) 177
AgsI TTSAA 5 cut(s) 269, 419, 478, 787, 807
AjiI CACGTC 1 cut(s) 882
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 3 cut(s) 584, 1058, 1179
AluI AGCT 3 cut(s) 584, 1058, 1179
Alw26I GTCTC 1 cut(s) 1032
AlwI GGATC 1 cut(s) 217
AoxI GGCC 3 cut(s) 488, 758, 1024
ApeKI GCWGC 4 cut(s) 402, 457, 503, 595
ApoI RAATTY 3 cut(s) 6, 214, 1049
AsiGI ACCGGT 1 cut(s) 177
Asp718I GGTACC 1 cut(s) 717
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 5 cut(s) 175, 348, 701, 758, 1024
AsuC2I CCSGG 1 cut(s) 722
AsuHPI GGTGA 4 cut(s) 292, 635, 736, 922
AvaII GGWCC 3 cut(s) 175, 348, 701
BanI GGYRCC 1 cut(s) 717
BanII GRGCYC 1 cut(s) 100
BbsI GAAGAC 2 cut(s) 243, 794
BbvI GCAGC 4 cut(s) 389, 469, 490, 582
BccI CCATC 4 cut(s) 242, 437, 647, 749
BceAI ACGGC 4 cut(s) 149, 271, 499, 934
BciT130I CCWGG 1 cut(s) 207
BciVI GTATCC 1 cut(s) 856
BcnI CCSGG 1 cut(s) 722
BcoDI GTCTC 1 cut(s) 1032
BfaI CTAG 6 cut(s) 129, 294, 650, 1101, 1139, 1180
BfmI CTRYAG 1 cut(s) 328
BfuI GTATCC 1 cut(s) 856
BisI GCNGC 5 cut(s) 400, 403, 458, 504, 596
BlsI GCNGC 5 cut(s) 401, 404, 459, 505, 597
Bme1390I CCNGG 2 cut(s) 207, 722
Bme18I GGWCC 3 cut(s) 175, 348, 701
BmgBI CACGTC 1 cut(s) 882
BmgT120I GGNCC 5 cut(s) 175, 348, 701, 758, 1024
BmiI GGNNCC 3 cut(s) 390, 719, 835
BmrFI CCNGG 2 cut(s) 207, 722
BmsI GCATC 2 cut(s) 597, 777
BpiI GAAGAC 2 cut(s) 243, 794
BpuEI CTTGAG 1 cut(s) 990
BpuMI CCSGG 1 cut(s) 722
BsaHI GRCGYC 2 cut(s) 123, 258
BsaJI CCNNGG 1 cut(s) 792
BsaWI WCCGGW 1 cut(s) 177
Bsc4I CCNNNNNNNGG 3 cut(s) 478, 698, 737
Bse118I RCCGGY 1 cut(s) 177
Bse1I ACTGG 1 cut(s) 1070
Bse3DI GCAATG 1 cut(s) 741
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 792
BseGI GGATG 3 cut(s) 253, 339, 612
BseLI CCNNNNNNNGG 3 cut(s) 478, 698, 737
BseMI GCAATG 1 cut(s) 741
BseMII CTCAG 2 cut(s) 124, 837
BseNI ACTGG 1 cut(s) 1070
BseXI GCAGC 4 cut(s) 389, 469, 490, 582
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 3 cut(s) 490, 760, 1026
BshNI GGYRCC 1 cut(s) 717
BshTI ACCGGT 1 cut(s) 177
BsiSI CCGG 2 cut(s) 178, 722
BslFI GGGAC 1 cut(s) 11
BslI CCNNNNNNNGG 3 cut(s) 478, 698, 737
BsmAI GTCTC 1 cut(s) 1032
BsmFI GGGAC 1 cut(s) 11
BsmI GAATGC 1 cut(s) 34
BsnI GGCC 3 cut(s) 490, 760, 1026
Bsp1286I GDGCHC 1 cut(s) 100
Bsp143I GATC 1 cut(s) 209
BspACI CCGC 7 cut(s) 32, 49, 142, 160, 245, 400, 413
BspANI GGCC 3 cut(s) 490, 760, 1026
BspCNI CTCAG 2 cut(s) 123, 836
BspFNI CGCG 1 cut(s) 162
BspLI GGNNCC 3 cut(s) 390, 719, 835
BspPI GGATC 1 cut(s) 217
BspT107I GGYRCC 1 cut(s) 717
BsrDI GCAATG 1 cut(s) 741
BsrFI RCCGGY 1 cut(s) 177
BsrI ACTGG 1 cut(s) 1070
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 1 cut(s) 792
BssMI GATC 1 cut(s) 209
BssNI GRCGYC 2 cut(s) 123, 258
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 1 cut(s) 783
Bst6I CTCTTC 1 cut(s) 498
BstACI GRCGYC 2 cut(s) 123, 258
BstC8I GCNNGC 4 cut(s) 42, 100, 136, 488
BstDEI CTNAG 4 cut(s) 110, 823, 1020, 1065
BstF5I GGATG 3 cut(s) 253, 339, 612
BstFNI CGCG 1 cut(s) 162
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 1 cut(s) 212
BstMAI GTCTC 1 cut(s) 1032
BstMBI GATC 1 cut(s) 209
BstMWI GCNNNNNNNGC 1 cut(s) 1106
BstNI CCWGG 1 cut(s) 207
BstSCI CCNGG 2 cut(s) 205, 720
BstSFI CTRYAG 1 cut(s) 328
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 4 cut(s) 389, 469, 490, 582
BstV2I GAAGAC 2 cut(s) 243, 794
BsuI GTATCC 1 cut(s) 856
BsuRI GGCC 3 cut(s) 490, 760, 1026
BtrI CACGTC 1 cut(s) 882
BtsCI GGATG 3 cut(s) 253, 339, 612
Cac8I GCNNGC 4 cut(s) 42, 100, 136, 488
CfoI GCGC 1 cut(s) 164
Cfr10I RCCGGY 1 cut(s) 177
Cfr13I GGNCC 5 cut(s) 175, 348, 701, 758, 1024
CpoI CGGWCCG 1 cut(s) 348
CseI GACGC 1 cut(s) 247
Csp6I GTAC 6 cut(s) 13, 105, 193, 718, 952, 1165
CspAI ACCGGT 1 cut(s) 177
CspI CGGWCCG 1 cut(s) 348
CviAII CATG 3 cut(s) 172, 599, 645
CviQI GTAC 6 cut(s) 13, 105, 193, 718, 952, 1165
DdeI CTNAG 4 cut(s) 110, 823, 1020, 1065
DpnI GATC 1 cut(s) 211
DpnII GATC 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
EciI GGCGGA 1 cut(s) 260
Eco147I AGGCCT 1 cut(s) 490
Eco24I GRGCYC 1 cut(s) 100
Eco47I GGWCC 3 cut(s) 175, 348, 701
Eco57I CTGAAG 1 cut(s) 552
EcoRII CCWGG 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 100
FaeI CATG 3 cut(s) 175, 602, 648
FaqI GGGAC 1 cut(s) 11
FatI CATG 3 cut(s) 171, 598, 644
FauI CCCGC 1 cut(s) 56
FblI GTMKAC 1 cut(s) 995
Fnu4HI GCNGC 5 cut(s) 400, 403, 458, 504, 596
FokI GGATG 3 cut(s) 260, 346, 619
FriOI GRGCYC 1 cut(s) 100
Fsp4HI GCNGC 5 cut(s) 400, 403, 458, 504, 596
FspBI CTAG 6 cut(s) 129, 294, 650, 1101, 1139, 1180
GlaI GCGC 1 cut(s) 163
GluI GCNGC 5 cut(s) 400, 403, 458, 504, 596
HaeIII GGCC 3 cut(s) 490, 760, 1026
HapII CCGG 2 cut(s) 178, 722
HgaI GACGC 1 cut(s) 247
HhaI GCGC 1 cut(s) 164
Hin1I GRCGYC 2 cut(s) 123, 258
Hin1II CATG 3 cut(s) 175, 602, 648
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HincII GTYRAC 2 cut(s) 169, 1123
HindII GTYRAC 2 cut(s) 169, 1123
HinfI GANTC 8 cut(s) 71, 289, 353, 515, 632, 677, 977, 1061
HpaI GTTAAC 2 cut(s) 169, 1123
HpaII CCGG 2 cut(s) 178, 722
HphI GGTGA 4 cut(s) 292, 635, 736, 922
Hpy166II GTNNAC 3 cut(s) 169, 996, 1123
Hpy188I TCNGA 5 cut(s) 113, 231, 352, 532, 874
Hpy188III TCNNGA 4 cut(s) 82, 704, 1007, 1139
Hpy8I GTNNAC 3 cut(s) 169, 996, 1123
Hpy99I CGWCG 4 cut(s) 193, 257, 263, 384
HpyAV CCTTC 6 cut(s) 87, 413, 527, 692, 823, 1004
HpyCH4III ACNGT 1 cut(s) 783
HpyCH4IV ACGT 3 cut(s) 123, 468, 881
HpyCH4V TGCA 3 cut(s) 405, 598, 768
HpyF10VI GCNNNNNNNGC 1 cut(s) 1106
HpyF3I CTNAG 4 cut(s) 110, 823, 1020, 1065
HpySE526I ACGT 3 cut(s) 123, 468, 881
Hsp92I GRCGYC 2 cut(s) 123, 258
Hsp92II CATG 3 cut(s) 175, 602, 648
HspAI GCGC 1 cut(s) 162
KpnI GGTACC 1 cut(s) 721
KspAI GTTAAC 2 cut(s) 169, 1123
Kzo9I GATC 1 cut(s) 209
Lsp1109I GCAGC 4 cut(s) 389, 469, 490, 582
LweI GCATC 2 cut(s) 597, 777
MaeI CTAG 6 cut(s) 129, 294, 650, 1101, 1139, 1180
MaeII ACGT 3 cut(s) 123, 468, 881
MaeIII GTNAC 3 cut(s) 124, 724, 1081
MalI GATC 1 cut(s) 211
MboI GATC 1 cut(s) 209
MhlI GDGCHC 1 cut(s) 100
MluCI AATT 6 cut(s) 6, 214, 670, 802, 848, 1049
MlyI GAGTC 1 cut(s) 362
MnlI CCTC 4 cut(s) 501, 583, 787, 928
MseI TTAA 5 cut(s) 168, 339, 687, 851, 1122
MspA1I CMGCKG 2 cut(s) 402, 1058
MspI CCGG 2 cut(s) 178, 722
MspR9I CCNGG 2 cut(s) 207, 722
Mva1269I GAATGC 1 cut(s) 34
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 1 cut(s) 1106
NciI CCSGG 1 cut(s) 722
NdeII GATC 1 cut(s) 209
NlaIII CATG 3 cut(s) 175, 602, 648
NlaIV GGNNCC 3 cut(s) 390, 719, 835
NmuCI GTSAC 2 cut(s) 124, 724
PceI AGGCCT 1 cut(s) 490
PcsI WCGNNNNNNNCGW 1 cut(s) 209
PctI GAATGC 1 cut(s) 34
PfeI GAWTC 7 cut(s) 71, 289, 515, 632, 677, 977, 1061
PflFI GACNNNGTC 1 cut(s) 257
PinAI ACCGGT 1 cut(s) 177
PkrI GCNGC 5 cut(s) 401, 404, 459, 505, 597
PleI GAGTC 1 cut(s) 361
PpsI GAGTC 1 cut(s) 361
Psp6I CCWGG 1 cut(s) 205
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 3 cut(s) 390, 719, 835
PspPI GGNCC 5 cut(s) 175, 348, 701, 758, 1024
PsyI GACNNNGTC 1 cut(s) 257
PvuII CAGCTG 1 cut(s) 1058
RsaI GTAC 6 cut(s) 14, 106, 194, 719, 953, 1166
RsaNI GTAC 6 cut(s) 13, 105, 193, 718, 952, 1165
Rsr2I CGGWCCG 1 cut(s) 348
RsrII CGGWCCG 1 cut(s) 348
SaqAI TTAA 5 cut(s) 168, 339, 687, 851, 1122
SatI GCNGC 5 cut(s) 400, 403, 458, 504, 596
Sau3AI GATC 1 cut(s) 209
Sau96I GGNCC 5 cut(s) 175, 348, 701, 758, 1024
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 2 cut(s) 207, 722
SduI GDGCHC 1 cut(s) 100
SfaNI GCATC 2 cut(s) 597, 777
SfcI CTRYAG 1 cut(s) 328
SinI GGWCC 3 cut(s) 175, 348, 701
SmlI CTYRAG 1 cut(s) 1005
SmoI CTYRAG 1 cut(s) 1005
Sse9I AATT 6 cut(s) 6, 214, 670, 802, 848, 1049
SseBI AGGCCT 1 cut(s) 490
SsiI CCGC 7 cut(s) 32, 49, 142, 160, 245, 400, 413
SspMI CTAG 6 cut(s) 129, 294, 650, 1101, 1139, 1180
StuI AGGCCT 1 cut(s) 490
StyD4I CCNGG 2 cut(s) 205, 720
TaaI ACNGT 1 cut(s) 783
TaiI ACGT 3 cut(s) 126, 471, 884
TaqI TCGA 3 cut(s) 69, 81, 212
TaqII GACCGA 2 cut(s) 336, 806
TasI AATT 6 cut(s) 6, 214, 670, 802, 848, 1049
TatI WGTACW 1 cut(s) 104
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 7 cut(s) 71, 289, 515, 632, 677, 977, 1061
Tru1I TTAA 5 cut(s) 168, 339, 687, 851, 1122
Tru9I TTAA 5 cut(s) 168, 339, 687, 851, 1122
TseFI GTSAC 2 cut(s) 124, 724
TseI GCWGC 4 cut(s) 402, 457, 503, 595
Tsp45I GTSAC 2 cut(s) 124, 724
TspDTI ATGAA 5 cut(s) 290, 444, 651, 669, 990
TspGWI ACGGA 1 cut(s) 177
Tth111I GACNNNGTC 1 cut(s) 257
VpaK11BI GGWCC 3 cut(s) 175, 348, 701
XapI RAATTY 3 cut(s) 6, 214, 1049
XbaI TCTAGA 1 cut(s) 1138
XcmI CCANNNNNNNNNTGG 1 cut(s) 711
XmiI GTMKAC 1 cut(s) 995
XspI CTAG 6 cut(s) 129, 294, 650, 1101, 1139, 1180
ZraI GACGTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.