FvH4_2g23700
BHLH Family

basic helix-loop-helix protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010418
Physical Location & Seq
Reverse (-)
19392632 .. 19399111
6480 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g23700.t3

Sequence Viewer

Length: 1935 bp
ATGGAAGTGAGCTCTGAAGAGGCGTCTCTTCAAAGGAGTCAACAGCTTAGGGAGCTCTACGACACACTGTCTGCCGGAGAAACAAACCAGCCAGCGCGCCGCCCTTGTGCCGCCTTGTCCCCGGAGGACTTAACCGAGTCGGAATGGTTCTACTTGATGTGTGTATCGTTCTCATTTCCCCCGGGAGTTGGGTTGCCGGGAAAAGCATACTCAAGGAGGCAACATGTATGGCTCACTGGTGCAAATGAAGTAGATAGCAAAACCTTCTCCAGAGCTATTTTAGCCAAGAGTGCTCGAGTTCAAACTGTAGTGTGCATTCCTCTCCTTGACGGCGTCGTCGAGCTAGGCACAACTGACAGGGTTCCAGAAGACCTAGCCTTCGTCCAACACGTCAAAACTTTCTTCGTCGATCACCACCACCTTCCACCGCCCAAGCCGGCCCTCTCGGAGCACTCCACCTCCAACCCCGCCACCTCCTCCGACCACCACCCCCGCTTCCATTCTCCTCACCTCACTGCCATCCGCAACAACGCGGCAGCAAACAACCCTCCTCCTCTCCACGCAGCCGCGCAAGAAGACGACGAGGACGAGGACGAAGAAGAAGACGACGAAGGAGAGTCCGACTCCGAAGCAGAAACCGGCCGTAACGGTAACGGACGTCCTCTCCCTCCTGCGGGAACAAACGTCCCCGCAGCCGAGCCTAGCGAGCTCATGCAGCTCGAAATGTCGGAAGACATCCGCCTCGGCTCCCCTGACGACGCCTCCAACAACTTGGACTCGGACTTCCACATGCTGGCCGTCAGCCAGTCCGCCAACGCCGCCGATCAGCAGCGCCAAGCCGACTCGTATCGCGCCGAGTCCGCCCGGCGCTGGCCTCCCGTCCAACCGCCTATGAGCGCCGTCCAACCACCACCCTCAGGACCGCTGGCGTTGGAGGAATTGAACCACGACGACACACACTACTCGGAAACGGTCTCCACCATACTTCAGACGCAAGCCACACGGTGGACCGATTCGTCGTCCAATGACTACGTCACCTACTCCAGCCAATCGGCTTTCGCCAAGTGGACGAGCCGAGCCGACCACCACCTTCTGCTGCCGGTTGAGGGCGCGTCCCAATGGCTACTAAAGTACATTTTGTTTAGTGTGCCCTTCCTGCACACGAAATATCGGGACGAGAATTCGCCGAAGTCGTCCCATGACGGCGAAGGCTCGACCCGGTTGAGGAAGGGGACGTCACAAGACGAGCTGAGTGCTAATCATGTGTTGGCAGAAAGGCGTAGAAGGGAGAAGCTTAACGAGAGGTTTATTATATTAAGGTCGTTGGTGCCATTTGTGACAAAAATGGACAAGGCCTCGATTTTGGGGGATACGATCGAGTATGTGAAGCAGCTGCGTAAGAAGATTCAGGACCTCGAGGCACGTAACGTGCACTTGGAGGACGATCAACAACGGACGAGATCGTTGGGGGAGATACAACGGTCTAGTAGCATGAAAGAGTTACGGAGCGGGCTCACGGTCACGGAGCGCTCCCGAGTGGGCCCGCCCGGGTCGGACAAGAGGAAGTTGAGGATCGTGGAAGGGAGTGGTGGTGTGGCGGTCGCTAAGCCTAAAGCGGTAGAGGAACCGACCCCGCCTCCGCCACCACCGGCACCTGAGCTGACCCCACCAACGCCCATGTTGACGGGGACGTCTCTAGAGGTGTCGATAATAGAGAGCGACGGATTGTTGGAGCTCCAATGCCCGTACCGAGAAGGGTTGTTACTTGATGTCATGCAGACACTACGAGATCTCCGTATTGAGACCACGGTTGTGCAATCCTCTTTGAACAGTGGCAATTTCGTAGCTGAGCTAAGGGCCAAGGTGGGCAAGAAAACAACCATCACACAAGTGAAGAGGGCAGTAAATCAAGTCATACCCCAGTCTGACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH bHLH-MYC_N

Protein Analysis

645

Amino Acids

71.18

Weight (kDa)

5.35

Isoelectric Point (pI)

63.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 11 - 193 2.9e-53 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 477 - 524 7e-13 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 624 - 678 5.9e-11 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 335, 1015, 1690
AatII GACGTC 3 cut(s) 661, 1238, 1694
AbsI CCTCGAGG 1 cut(s) 1415
AccB1I GGYRCC 2 cut(s) 1327, 1651
AccB7I CCANNNNNTGG 2 cut(s) 793, 1005
AccBSI CCGCTC 1 cut(s) 1509
AccII CGCG 5 cut(s) 97, 533, 569, 852, 1112
AclWI GGATC 1 cut(s) 1580
AcoI YGGCCR 2 cut(s) 640, 795
AcsI RAATTY 1 cut(s) 1180
AcuI CTGAAG 2 cut(s) 36, 971
AcyI GRCGYC 6 cut(s) 23, 333, 658, 759, 1235, 1691
AdeI CACNNNGTG 1 cut(s) 1005
AfaI GTAC 2 cut(s) 1133, 1748
AfeI AGCGCT 1 cut(s) 1529
AfiI CCNNNNNNNGG 9 cut(s) 188, 673, 674, 793, 870, 917, 1005, 1106, 1224
AflIII ACRYGT 2 cut(s) 223, 388
AgsI TTSAA 4 cut(s) 32, 302, 943, 1828
AhdI GACNNNNNGTC 1 cut(s) 67
AjiI CACGTC 1 cut(s) 391
AleI CACNNNNGTG 2 cut(s) 1811, 1889
Alw21I GWGCWC 7 cut(s) 14, 57, 295, 453, 711, 1434, 1737
Alw26I GTCTC 4 cut(s) 30, 979, 1698, 1796
Alw44I GTGCAC 1 cut(s) 1430
AlwI GGATC 1 cut(s) 1580
Ama87I CYCGRG 5 cut(s) 181, 294, 1415, 1533, 1547
Aor51HI AGCGCT 1 cut(s) 1529
AoxI GGCC 7 cut(s) 438, 640, 795, 872, 1353, 1540, 1857
ApaI GGGCCC 1 cut(s) 1544
ApaLI GTGCAC 1 cut(s) 1430
ApeKI GCWGC 8 cut(s) 536, 563, 692, 715, 829, 1096, 1390, 1393
ApoI RAATTY 1 cut(s) 1180
ArsI GACNNNNNNTTYG 2 cut(s) 362, 394
AspLEI GCGC 9 cut(s) 97, 99, 571, 834, 854, 870, 899, 1112, 1530
AspS9I GGNCC 7 cut(s) 439, 920, 1008, 1411, 1540, 1541, 1857
AsuC2I CCSGG 8 cut(s) 122, 182, 183, 198, 865, 1219, 1548, 1549
AsuHPI GGTGA 3 cut(s) 404, 500, 1027
AvaI CYCGRG 5 cut(s) 181, 294, 1415, 1533, 1547
AvaII GGWCC 3 cut(s) 920, 1008, 1411
AxyI CCTNAGG 1 cut(s) 916
BaeGI GKGCMC 3 cut(s) 1152, 1434, 1544
BaeI ACNNNNGTAYC 2 cut(s) 1466, 1499
BanI GGYRCC 2 cut(s) 1327, 1651
BanII GRGCYC 6 cut(s) 14, 57, 711, 1515, 1544, 1737
BarI GAAGNNNNNNTAC 2 cut(s) 1746, 1778
BbsI GAAGAC 4 cut(s) 375, 582, 609, 738
Bbv12I GWGCWC 7 cut(s) 14, 57, 295, 453, 711, 1434, 1737
BbvI GCAGC 8 cut(s) 548, 575, 704, 727, 841, 1083, 1380, 1402
BccI CCATC 2 cut(s) 527, 1889
BceAI ACGGC 5 cut(s) 346, 627, 782, 884, 1219
BcgI CGANNNNNNTGC 2 cut(s) 1235, 1269
BciVI GTATCC 1 cut(s) 1363
BcnI CCSGG 8 cut(s) 122, 182, 183, 198, 865, 1219, 1548, 1549
BcoDI GTCTC 4 cut(s) 30, 979, 1698, 1796
BfaI CTAG 5 cut(s) 344, 374, 702, 1485, 1697
BfmI CTRYAG 1 cut(s) 306
BfoI RGCGCY 4 cut(s) 835, 871, 900, 1531
BfuI GTATCC 1 cut(s) 1363
BglII AGATCT 1 cut(s) 1789
BlpI GCTNAGC 2 cut(s) 1605, 1848
Bme1390I CCNGG 8 cut(s) 122, 182, 183, 198, 865, 1219, 1548, 1549
Bme18I GGWCC 3 cut(s) 920, 1008, 1411
BmeRI GACNNNNNGTC 1 cut(s) 67
BmeT110I CYCGRG 5 cut(s) 181, 294, 1415, 1533, 1547
BmgBI CACGTC 1 cut(s) 391
BmgT120I GGNCC 7 cut(s) 439, 920, 1008, 1411, 1540, 1541, 1857
BmiI GGNNCC 6 cut(s) 363, 748, 1329, 1542, 1626, 1653
BmrFI CCNGG 8 cut(s) 122, 182, 183, 198, 865, 1219, 1548, 1549
BmrI ACTGGG 1 cut(s) 1915
BmuI ACTGGG 1 cut(s) 1915
BpiI GAAGAC 4 cut(s) 375, 582, 609, 738
BplI GAGNNNNNCTC 2 cut(s) 608, 640
BpmI CTGGAG 2 cut(s) 253, 1027
Bpu10I CCTNAGC 3 cut(s) 47, 1656, 1853
Bpu1102I GCTNAGC 2 cut(s) 1605, 1848
BpuEI CTTGAG 1 cut(s) 196
BpuMI CCSGG 8 cut(s) 122, 182, 183, 198, 865, 1219, 1548, 1549
BsaAI YACGTR 1 cut(s) 1424
BsaHI GRCGYC 6 cut(s) 23, 333, 658, 759, 1235, 1691
BsaI GGTCTC 2 cut(s) 979, 1796
BsaJI CCNNGG 7 cut(s) 120, 180, 181, 742, 1547, 1806, 1860
Bsc4I CCNNNNNNNGG 9 cut(s) 188, 673, 674, 793, 870, 917, 1005, 1106, 1224
Bse118I RCCGGY 4 cut(s) 436, 638, 1099, 1648
Bse1I ACTGG 3 cut(s) 241, 805, 1921
Bse21I CCTNAGG 1 cut(s) 916
BseDI CCNNGG 7 cut(s) 120, 180, 181, 742, 1547, 1806, 1860
BseGI GGATG 2 cut(s) 519, 735
BseLI CCNNNNNNNGG 9 cut(s) 188, 673, 674, 793, 870, 917, 1005, 1106, 1224
BseMII CTCAG 4 cut(s) 930, 1241, 1647, 1839
BseNI ACTGG 3 cut(s) 241, 805, 1921
BsePI GCGCGC 1 cut(s) 95
BseRI GAGGAG 4 cut(s) 466, 495, 540, 543
BseSI GKGCMC 3 cut(s) 1152, 1434, 1544
BseX3I CGGCCG 1 cut(s) 640
BseXI GCAGC 8 cut(s) 548, 575, 704, 727, 841, 1083, 1380, 1402
BsgI GTGCAG 1 cut(s) 1142
Bsh1236I CGCG 5 cut(s) 97, 533, 569, 852, 1112
Bsh1285I CGRYCG 3 cut(s) 643, 1377, 1602
BshFI GGCC 7 cut(s) 440, 642, 797, 874, 1355, 1542, 1859
BshNI GGYRCC 2 cut(s) 1327, 1651
BsiEI CGRYCG 3 cut(s) 643, 1377, 1602
BsiHKAI GWGCWC 7 cut(s) 14, 57, 295, 453, 711, 1434, 1737
BsiHKCI CYCGRG 5 cut(s) 181, 294, 1415, 1533, 1547
BslFI GGGAC 7 cut(s) 103, 671, 1099, 1180, 1187, 1246, 1702
BslI CCNNNNNNNGG 9 cut(s) 188, 673, 674, 793, 870, 917, 1005, 1106, 1224
BsmAI GTCTC 4 cut(s) 30, 979, 1698, 1796
BsmBI CGTCTC 2 cut(s) 30, 1698
BsmFI GGGAC 7 cut(s) 103, 671, 1099, 1180, 1187, 1246, 1702
BsmI GAATGC 1 cut(s) 315
BsnI GGCC 7 cut(s) 440, 642, 797, 874, 1355, 1542, 1859
Bso31I GGTCTC 2 cut(s) 979, 1796
BsoBI CYCGRG 5 cut(s) 181, 294, 1415, 1533, 1547
Bsp120I GGGCCC 1 cut(s) 1540
Bsp143I GATC 7 cut(s) 409, 823, 1374, 1444, 1460, 1572, 1789
Bsp1720I GCTNAGC 2 cut(s) 1605, 1848
BspANI GGCC 7 cut(s) 440, 642, 797, 874, 1355, 1542, 1859
BspCNI CTCAG 4 cut(s) 929, 1242, 1648, 1840
BspFNI CGCG 5 cut(s) 97, 533, 569, 852, 1112
BspLI GGNNCC 6 cut(s) 363, 748, 1329, 1542, 1626, 1653
BspPI GGATC 1 cut(s) 1580
BspT107I GGYRCC 2 cut(s) 1327, 1651
BspTNI GGTCTC 2 cut(s) 979, 1796
BsrBI CCGCTC 1 cut(s) 1509
BsrFI RCCGGY 4 cut(s) 436, 638, 1099, 1648
BsrI ACTGG 3 cut(s) 241, 805, 1921
BssAI RCCGGY 4 cut(s) 436, 638, 1099, 1648
BssECI CCNNGG 7 cut(s) 120, 180, 181, 742, 1547, 1806, 1860
BssHII GCGCGC 1 cut(s) 95
BssMI GATC 7 cut(s) 409, 823, 1374, 1444, 1460, 1572, 1789
BssNI GRCGYC 6 cut(s) 23, 333, 658, 759, 1235, 1691
BssT1I CCWWGG 1 cut(s) 1860
Bst4CI ACNGT 9 cut(s) 69, 307, 650, 973, 1005, 1482, 1519, 1810, 1832
Bst6I CTCTTC 3 cut(s) 12, 33, 1889
BstACI GRCGYC 6 cut(s) 23, 333, 658, 759, 1235, 1691
BstBAI YACGTR 1 cut(s) 1424
BstDEI CTNAG 7 cut(s) 47, 916, 1250, 1605, 1656, 1848, 1853
BstDSI CCRYGG 1 cut(s) 1806
BstF5I GGATG 2 cut(s) 519, 735
BstFNI CGCG 5 cut(s) 97, 533, 569, 852, 1112
BstH2I RGCGCY 4 cut(s) 835, 871, 900, 1531
BstHHI GCGC 9 cut(s) 97, 99, 571, 834, 854, 870, 899, 1112, 1530
BstKTI GATC 7 cut(s) 412, 826, 1377, 1447, 1463, 1575, 1792
BstMAI GTCTC 4 cut(s) 30, 979, 1698, 1796
BstMBI GATC 7 cut(s) 409, 823, 1374, 1444, 1460, 1572, 1789
BstMCI CGRYCG 3 cut(s) 643, 1377, 1602
BstMWI GCNNNNNNNGC 8 cut(s) 52, 281, 290, 706, 715, 818, 860, 1156
BstNSI RCATGY 2 cut(s) 227, 793
BstSCI CCNGG 8 cut(s) 120, 180, 181, 196, 863, 1217, 1546, 1547
BstSFI CTRYAG 1 cut(s) 306
BstSLI GKGCMC 3 cut(s) 1152, 1434, 1544
BstUI CGCG 5 cut(s) 97, 533, 569, 852, 1112
BstV1I GCAGC 8 cut(s) 548, 575, 704, 727, 841, 1083, 1380, 1402
BstV2I GAAGAC 4 cut(s) 375, 582, 609, 738
BstX2I RGATCY 1 cut(s) 1789
BstXI CCANNNNNNTGG 1 cut(s) 772
BstYI RGATCY 1 cut(s) 1789
BstZI CGGCCG 1 cut(s) 640
Bsu36I CCTNAGG 1 cut(s) 916
BsuI GTATCC 1 cut(s) 1363
BsuRI GGCC 7 cut(s) 440, 642, 797, 874, 1355, 1542, 1859
BtgI CCRYGG 1 cut(s) 1806
BtrI CACGTC 1 cut(s) 391
BtsCI GGATG 2 cut(s) 519, 735
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 4 cut(s) 65, 234, 513, 1837
CfoI GCGC 9 cut(s) 97, 99, 571, 834, 854, 870, 899, 1112, 1530
Cfr10I RCCGGY 4 cut(s) 436, 638, 1099, 1648
Cfr13I GGNCC 7 cut(s) 439, 920, 1008, 1411, 1540, 1541, 1857
Cfr9I CCCGGG 2 cut(s) 181, 1547
CseI GACGC 5 cut(s) 12, 322, 767, 1000, 1101
Csp6I GTAC 2 cut(s) 1132, 1747
CviAII CATG 8 cut(s) 224, 712, 790, 1199, 1262, 1492, 1678, 1774
CviQI GTAC 2 cut(s) 1132, 1747
DdeI CTNAG 7 cut(s) 47, 916, 1250, 1605, 1656, 1848, 1853
DpnI GATC 7 cut(s) 411, 825, 1376, 1446, 1462, 1574, 1791
DpnII GATC 7 cut(s) 409, 823, 1374, 1444, 1460, 1572, 1789
DraIII CACNNNGTG 1 cut(s) 1005
DrdI GACNNNNNNGTC 3 cut(s) 335, 1015, 1690
DriI GACNNNNNGTC 1 cut(s) 67
DseDI GACNNNNNNGTC 3 cut(s) 335, 1015, 1690
EaeI YGGCCR 2 cut(s) 640, 795
EagI CGGCCG 1 cut(s) 640
Eam1104I CTCTTC 3 cut(s) 12, 33, 1889
Eam1105I GACNNNNNGTC 1 cut(s) 67
EarI CTCTTC 3 cut(s) 12, 33, 1889
EciI GGCGGA 4 cut(s) 728, 799, 850, 1629
Ecl136II GAGCTC 4 cut(s) 12, 55, 709, 1735
EclXI CGGCCG 1 cut(s) 640
Eco130I CCWWGG 1 cut(s) 1860
Eco147I AGGCCT 1 cut(s) 1355
Eco24I GRGCYC 6 cut(s) 14, 57, 711, 1515, 1544, 1737
Eco31I GGTCTC 2 cut(s) 979, 1796
Eco47I GGWCC 3 cut(s) 920, 1008, 1411
Eco47III AGCGCT 1 cut(s) 1529
Eco52I CGGCCG 1 cut(s) 640
Eco53kI GAGCTC 4 cut(s) 12, 55, 709, 1735
Eco57I CTGAAG 2 cut(s) 36, 971
Eco81I CCTNAGG 1 cut(s) 916
Eco88I CYCGRG 5 cut(s) 181, 294, 1415, 1533, 1547
EcoICRI GAGCTC 4 cut(s) 12, 55, 709, 1735
EcoO109I RGGNCCY 1 cut(s) 1411
EcoRI GAATTC 1 cut(s) 1180
EcoT14I CCWWGG 1 cut(s) 1860
EcoT38I GRGCYC 6 cut(s) 14, 57, 711, 1515, 1544, 1737
ErhI CCWWGG 1 cut(s) 1860
Esp3I CGTCTC 2 cut(s) 30, 1698
FaeI CATG 8 cut(s) 227, 715, 793, 1202, 1265, 1495, 1681, 1777
FaqI GGGAC 7 cut(s) 103, 671, 1099, 1180, 1187, 1246, 1702
FatI CATG 8 cut(s) 223, 711, 789, 1198, 1261, 1491, 1677, 1773
FauI CCCGC 7 cut(s) 475, 500, 667, 697, 1502, 1551, 1641
FokI GGATG 2 cut(s) 506, 722
FriOI GRGCYC 6 cut(s) 14, 57, 711, 1515, 1544, 1737
FspBI CTAG 5 cut(s) 344, 374, 702, 1485, 1697
GlaI GCGC 9 cut(s) 96, 98, 570, 833, 853, 869, 898, 1111, 1529
GsuI CTGGAG 2 cut(s) 253, 1027
HaeII RGCGCY 4 cut(s) 835, 871, 900, 1531
HaeIII GGCC 7 cut(s) 440, 642, 797, 874, 1355, 1542, 1859
HgaI GACGC 5 cut(s) 12, 322, 767, 1000, 1101
HhaI GCGC 9 cut(s) 97, 99, 571, 834, 854, 870, 899, 1112, 1530
Hin1I GRCGYC 6 cut(s) 23, 333, 658, 759, 1235, 1691
Hin1II CATG 8 cut(s) 227, 715, 793, 1202, 1265, 1495, 1681, 1777
Hin6I GCGC 9 cut(s) 95, 97, 569, 832, 852, 868, 897, 1110, 1528
HinP1I GCGC 9 cut(s) 95, 97, 569, 832, 852, 868, 897, 1110, 1528
HincII GTYRAC 2 cut(s) 41, 1683
HindII GTYRAC 2 cut(s) 41, 1683
HindIII AAGCTT 1 cut(s) 1292
HphI GGTGA 3 cut(s) 404, 500, 1027
Hpy166II GTNNAC 5 cut(s) 41, 1008, 1068, 1432, 1683
Hpy188III TCNNGA 7 cut(s) 270, 365, 918, 1172, 1409, 1533, 1697
Hpy8I GTNNAC 5 cut(s) 41, 1008, 1068, 1432, 1683
Hpy99I CGWCG 9 cut(s) 338, 341, 410, 584, 611, 761, 953, 1021, 1724
HpyCH4III ACNGT 9 cut(s) 69, 307, 650, 973, 1005, 1482, 1519, 1810, 1832
HpyCH4IV ACGT 8 cut(s) 390, 658, 684, 1032, 1235, 1423, 1428, 1691
HpyCH4V TGCA 7 cut(s) 242, 315, 715, 1159, 1432, 1777, 1816
HpyF10VI GCNNNNNNNGC 8 cut(s) 52, 281, 290, 706, 715, 818, 860, 1156
HpyF3I CTNAG 7 cut(s) 47, 916, 1250, 1605, 1656, 1848, 1853
HpySE526I ACGT 8 cut(s) 390, 658, 684, 1032, 1235, 1423, 1428, 1691
Hsp92I GRCGYC 6 cut(s) 23, 333, 658, 759, 1235, 1691
Hsp92II CATG 8 cut(s) 227, 715, 793, 1202, 1265, 1495, 1681, 1777
HspAI GCGC 9 cut(s) 95, 97, 569, 832, 852, 868, 897, 1110, 1528
KroI GCCGGC 1 cut(s) 436
KroNI GCCGGC 1 cut(s) 438
Kzo9I GATC 7 cut(s) 409, 823, 1374, 1444, 1460, 1572, 1789
LmnI GCTCC 8 cut(s) 52, 448, 752, 1506, 1525, 1535, 1732, 1740
Lsp1109I GCAGC 8 cut(s) 548, 575, 704, 727, 841, 1083, 1380, 1402
MaeI CTAG 5 cut(s) 344, 374, 702, 1485, 1697
MaeII ACGT 8 cut(s) 390, 658, 684, 1032, 1235, 1423, 1428, 1691
MaeIII GTNAC 9 cut(s) 644, 650, 1033, 1236, 1336, 1424, 1500, 1519, 1761
MalI GATC 7 cut(s) 411, 825, 1376, 1446, 1462, 1574, 1791
MbiI CCGCTC 1 cut(s) 1509
MboI GATC 7 cut(s) 409, 823, 1374, 1444, 1460, 1572, 1789
MflI RGATCY 1 cut(s) 1789
MluCI AATT 3 cut(s) 938, 1180, 1837
MlyI GAGTC 8 cut(s) 46, 146, 617, 626, 770, 836, 866, 1922
MroNI GCCGGC 1 cut(s) 436
MseI TTAA 4 cut(s) 131, 1296, 1316, 1933
MslI CAYNNNNRTG 2 cut(s) 1811, 1889
MspA1I CMGCKG 2 cut(s) 925, 1393
MspR9I CCNGG 8 cut(s) 122, 182, 183, 198, 865, 1219, 1548, 1549
Mva1269I GAATGC 1 cut(s) 315
MvnI CGCG 5 cut(s) 97, 533, 569, 852, 1112
MwoI GCNNNNNNNGC 8 cut(s) 52, 281, 290, 706, 715, 818, 860, 1156
NaeI GCCGGC 1 cut(s) 438
NciI CCSGG 8 cut(s) 122, 182, 183, 198, 865, 1219, 1548, 1549
NdeII GATC 7 cut(s) 409, 823, 1374, 1444, 1460, 1572, 1789
NgoMIV GCCGGC 1 cut(s) 436
NlaIII CATG 8 cut(s) 227, 715, 793, 1202, 1265, 1495, 1681, 1777
NlaIV GGNNCC 6 cut(s) 363, 748, 1329, 1542, 1626, 1653
NmeAIII GCCGAG 4 cut(s) 721, 723, 880, 1100
NmuCI GTSAC 4 cut(s) 1033, 1236, 1336, 1519
NspI RCATGY 2 cut(s) 227, 793
OliI CACNNNNGTG 2 cut(s) 1811, 1889
PaeR7I CTCGAG 2 cut(s) 294, 1415
PauI GCGCGC 1 cut(s) 95
PceI AGGCCT 1 cut(s) 1355
PciI ACATGT 1 cut(s) 223
PcsI WCGNNNNNNNCGW 5 cut(s) 336, 387, 585, 1190, 1792
PctI GAATGC 1 cut(s) 315
PdiI GCCGGC 1 cut(s) 438
PfeI GAWTC 2 cut(s) 1013, 1405
PflFI GACNNNGTC 2 cut(s) 332, 1031
PflMI CCANNNNNTGG 2 cut(s) 793, 1005
Ple19I CGATCG 1 cut(s) 1377
PleI GAGTC 8 cut(s) 45, 145, 617, 625, 770, 836, 865, 1922
PpsI GAGTC 8 cut(s) 45, 145, 617, 625, 770, 836, 865, 1922
Ppu21I YACGTR 1 cut(s) 1424
PpuMI RGGWCCY 1 cut(s) 1411
PscI ACATGT 1 cut(s) 223
Psp124BI GAGCTC 4 cut(s) 14, 57, 711, 1737
Psp5II RGGWCCY 1 cut(s) 1411
PspN4I GGNNCC 6 cut(s) 363, 748, 1329, 1542, 1626, 1653
PspOMI GGGCCC 1 cut(s) 1540
PspPI GGNCC 7 cut(s) 439, 920, 1008, 1411, 1540, 1541, 1857
PspPPI RGGWCCY 1 cut(s) 1411
PspXI VCTCGAGB 2 cut(s) 294, 1415
PsuI RGATCY 1 cut(s) 1789
PsyI GACNNNGTC 2 cut(s) 332, 1031
PteI GCGCGC 1 cut(s) 95
PvuI CGATCG 1 cut(s) 1377
PvuII CAGCTG 1 cut(s) 1393
RsaI GTAC 2 cut(s) 1133, 1748
RsaNI GTAC 2 cut(s) 1132, 1747
RseI CAYNNNNRTG 2 cut(s) 1811, 1889
SacI GAGCTC 4 cut(s) 14, 57, 711, 1737
SaqAI TTAA 4 cut(s) 131, 1296, 1316, 1933
Sau3AI GATC 7 cut(s) 409, 823, 1374, 1444, 1460, 1572, 1789
Sau96I GGNCC 7 cut(s) 439, 920, 1008, 1411, 1540, 1541, 1857
SchI GAGTC 8 cut(s) 46, 146, 617, 626, 770, 836, 866, 1922
ScrFI CCNGG 8 cut(s) 122, 182, 183, 198, 865, 1219, 1548, 1549
SfcI CTRYAG 1 cut(s) 306
Sfr274I CTCGAG 2 cut(s) 294, 1415
SinI GGWCC 3 cut(s) 920, 1008, 1411
SlaI CTCGAG 2 cut(s) 294, 1415
SmaI CCCGGG 2 cut(s) 183, 1549
SmiMI CAYNNNNRTG 2 cut(s) 1811, 1889
SmlI CTYRAG 3 cut(s) 211, 294, 1415
SmoI CTYRAG 3 cut(s) 211, 294, 1415
Sse9I AATT 3 cut(s) 938, 1180, 1837
SseBI AGGCCT 1 cut(s) 1355
SspMI CTAG 5 cut(s) 344, 374, 702, 1485, 1697
SstI GAGCTC 4 cut(s) 14, 57, 711, 1737
StuI AGGCCT 1 cut(s) 1355
StyD4I CCNGG 8 cut(s) 120, 180, 181, 196, 863, 1217, 1546, 1547
StyI CCWWGG 1 cut(s) 1860
TaaI ACNGT 9 cut(s) 69, 307, 650, 973, 1005, 1482, 1519, 1810, 1832
TaiI ACGT 8 cut(s) 393, 661, 687, 1035, 1238, 1426, 1431, 1694
TaqI TCGA 9 cut(s) 295, 339, 408, 720, 1214, 1358, 1377, 1416, 1706
TaqII GACCGA 1 cut(s) 1025
TasI AATT 3 cut(s) 938, 1180, 1837
TatI WGTACW 1 cut(s) 1131
TauI GCSGC 5 cut(s) 102, 113, 536, 569, 821
TfiI GAWTC 2 cut(s) 1013, 1405
Tru1I TTAA 4 cut(s) 131, 1296, 1316, 1933
Tru9I TTAA 4 cut(s) 131, 1296, 1316, 1933
TscAI CASTG 4 cut(s) 72, 241, 520, 1837
TseFI GTSAC 4 cut(s) 1033, 1236, 1336, 1519
TseI GCWGC 8 cut(s) 536, 563, 692, 715, 829, 1096, 1390, 1393
Tsp45I GTSAC 4 cut(s) 1033, 1236, 1336, 1519
TspDTI ATGAA 2 cut(s) 261, 1508
TspGWI ACGGA 6 cut(s) 669, 1468, 1519, 1538, 1737, 1784
TspMI CCCGGG 2 cut(s) 181, 1547
TspRI CASTG 4 cut(s) 72, 241, 520, 1837
Tth111I GACNNNGTC 2 cut(s) 332, 1031
Van91I CCANNNNNTGG 2 cut(s) 793, 1005
VneI GTGCAC 1 cut(s) 1430
VpaK11BI GGWCC 3 cut(s) 920, 1008, 1411
XapI RAATTY 1 cut(s) 1180
XbaI TCTAGA 1 cut(s) 1696
XceI RCATGY 2 cut(s) 227, 793
XhoI CTCGAG 2 cut(s) 294, 1415
XmaI CCCGGG 2 cut(s) 181, 1547
XspI CTAG 5 cut(s) 344, 374, 702, 1485, 1697
ZraI GACGTC 3 cut(s) 659, 1236, 1692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.