Rh6DG329400
BHLH Family

basic helix-loop-helix protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0010418
Physical Location & Seq
Reverse (-)
53390620 .. 53396068
5449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG329400.1

Sequence Viewer

Length: 2085 bp
ATGGCTACACCGCCACCGAGTAGTAGCAGGCTCCGTGGCATGTTACAGTCTGCAGTGCAATCTGTTCAATGGACTTACAGTCTCTTTTGGCAAATCTGTCCCCAACAAGGGATGTTAATATGGGCAGATGGGTATTACAATGGGGCAATCAAGACGAGGAAGACTGTGCAACCTATGGAAGTGAGCTCTGAGGAGGCATCTCTTCAAAGGAGCCAACAGCTTAGAGAGCTCTACGACTCACTGTCCGCGGGAGAAACAAACCAGCCAGCTCGCCGCCCTTGTGCTGCCTTGTCCCCGGAGGACTTAACTGAGTCGGAATGGTTCTACTTGATGTGTGTATCTTTCTCATTTCCCCCGGGAGTTGGGTTGCCGGGAAAAGCATACACAAGGAGGCAACACGTATGGCTCACTGGTGCAAATGAAGTAGATAGCAAAACCTTCTCCAGAGCTATTTTAGCCAAGAGTGCTCGCGTTCAAACTGTAGTGTGCATTCCTCTCTTAGACGGCGTGGTTGAGCTAGGCACAACAGATAGGGTTCCAGAAGACCTTGCCTTCGTCCAACACGTCAAAACTTTCTTCGTCGACCACCACCACCTTCCACCACCAAAGCCCGCCCTCTCGGAGCACTCCACCTCCAACCCCGCCACCTCCTCCGACCACCACCCCCGTTTCCACTCTCCACATCTCACCGCCATCCGCAACAACGCTGCCGCAAACAACGCCCAGCCCCTCCACGCAGCCGCCCAAGAAGACGAAGAGGACGAGGACGAGGAAGAAGACGACCAGGACGAAGAAGGAGAGTCCGACTCCGAAGCCGAAACCGGCCGTAACGGTCGTCCTCTCGCTGCTGCAGCGGGACCCAACGTTCCCGCAGCCGAGCCGAGCGAGCTAATGCAGCTCGAAATGTCCGAAGACATCCGCCTCGGCTCCCCCGACGACGCCTCCAACAATTTGGACTCGGACTTCCACATGCTGGCCGTCAGCCAGTCCGCCAACGCCGCCAATCAGCAGCGCCAAACAGACTCGTATCGCGCCGAGTCGACCCGGCGGTGGCCGCCGGTCCAACCGCCAATGACCGCTGTCCAACCACCTCCCTCAGGACCGCTGGCATTGGAGGAATTGACCCACGACGACACACACTATTCGGAAACAGTGTCGACCATACTTCAAACCACCAGGTGGACCGATTCGTCGTCCAATGACTACGTCACCTACTCCACCCAATCAGCTTTCGCCAAGTGGACGAGCCGGGCTGACCACCACCTCTTGGTTCCGGTCGAGGGCACATCCCAGTGGCTCCTAAAGTACATTTTGTTTAGTGTACCGTTCCTACATACGAAATATCGGGACGAGAATTCCCCCAAGTCTCACGACGTCGACGGCTCGAGCCGGTTGAGGAAGGGGACGTCACAAGACGAGCTAAGTGCTAACCATGTGTTAGCGGAGCGGCGTCGTAGAGAGAAGCTTAATGAGAGGTTTATTATATTGAGGTCTTTGGTGCCTTTTGTGACAAAAATGGACAAGGCCTCGATTTTGGGGGATACGATCGAGTACGTGAAGCAACTGCGTAAGAAGATTCAGGATCTCGAGGCACGTAACGTGCATTTGGAGGACGATCAACGCACGAGATCGGCGGGGGAGATGCAACGGTCGAGTAGCATGAAAGAGCTGCGAAGCGGGCTCACGGTGACGGAGCGCTCCCGCGTGGGCCCGCCCGGGTCGGACAAGAGGAAGTTGAGGATTGTGGAGGGGAGTGGCGGAGCGGCGATCGCTAAGCCTAAACTGATAGAGGAACCGACACCACCTCCGCCACCACCGGCACCGGCACCGGAACCGGCCCCGCCAACGCCCATGTTGACGGGGACGTCTCTAGAGGTGTCGATAATAGAGAGCGATGGATTGTTGGAGCTCCAATGCCCGTACAGAGAAGGGTTGTTACTTGATGTCATGCAAACCCTACGAGATCTCCGAATTGAGACCACGGTTGTGCAATCCTCGTTGAATAGTGGCACCTTCGTAGCTGAGCTAAGGGCCAAGGTAATACAATTTACTTTTTTACCGCCTTTAACCATAGCCCTGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH bHLH-MYC_N

Protein Analysis

694

Amino Acids

77.0

Weight (kDa)

5.39

Isoelectric Point (pI)

64.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 11 - 193 3.8e-53 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 476 - 523 7e-13 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 624 - 678 4.8e-11 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1189, 1864
AatII GACGTC 3 cut(s) 1377, 1409, 1868
AccB1I GGYRCC 4 cut(s) 1498, 1819, 1825, 2009
AccB7I CCANNNNNTGG 3 cut(s) 973, 1179, 1267
AccBSI CCGCTC 2 cut(s) 1447, 1763
AccI GTMKAC 4 cut(s) 582, 1040, 1157, 1377
AccII CGCG 4 cut(s) 248, 471, 1032, 1704
AclI AACGTT 1 cut(s) 864
AclWI GGATC 1 cut(s) 1590
AcoI YGGCCR 3 cut(s) 823, 975, 1052
AcsI RAATTY 1 cut(s) 1354
AcyI GRCGYC 5 cut(s) 939, 1374, 1406, 1450, 1865
AdeI CACNNNGTG 1 cut(s) 1179
AfaI GTAC 4 cut(s) 1307, 1323, 1553, 1922
AfeI AGCGCT 1 cut(s) 1697
AfiI CCNNNNNNNGG 9 cut(s) 107, 108, 362, 973, 1050, 1097, 1179, 1267, 1280
AflIII ACRYGT 2 cut(s) 397, 562
AgsI TTSAA 5 cut(s) 68, 206, 476, 1169, 2002
AhdI GACNNNNNGTC 2 cut(s) 78, 241
AjiI CACGTC 1 cut(s) 565
AjnI CCWGG 2 cut(s) 783, 1175
AleI CACNNNNGTG 1 cut(s) 1985
AloI GAACNNNNNNTCC 2 cut(s) 849, 881
Alw21I GWGCWC 5 cut(s) 188, 231, 469, 627, 1911
Alw26I GTCTC 4 cut(s) 86, 1371, 1872, 1970
AlwI GGATC 1 cut(s) 1590
Ama87I CYCGRG 4 cut(s) 355, 1384, 1586, 1715
Aor51HI AGCGCT 1 cut(s) 1697
AoxI GGCC 7 cut(s) 823, 975, 1052, 1524, 1708, 1836, 2031
ApaI GGGCCC 1 cut(s) 1712
ApoI RAATTY 1 cut(s) 1354
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
AsiSI GCGATCGC 1 cut(s) 1770
AspLEI GCGC 3 cut(s) 1014, 1034, 1698
AspS9I GGNCC 8 cut(s) 857, 1060, 1100, 1182, 1708, 1709, 1837, 2031
AsuC2I CCSGG 8 cut(s) 296, 356, 357, 372, 1045, 1250, 1716, 1717
AsuHPI GGTGA 3 cut(s) 679, 1201, 1699
AvaI CYCGRG 4 cut(s) 355, 1384, 1586, 1715
AvaII GGWCC 4 cut(s) 857, 1060, 1100, 1182
AxyI CCTNAGG 1 cut(s) 1096
BaeGI GKGCMC 2 cut(s) 1286, 1712
BanI GGYRCC 4 cut(s) 1498, 1819, 1825, 2009
BanII GRGCYC 5 cut(s) 188, 231, 1683, 1712, 1911
BarI GAAGNNNNNNTAC 2 cut(s) 1920, 1952
BauI CACGAG 1 cut(s) 1624
BbsI GAAGAC 5 cut(s) 167, 549, 756, 783, 918
Bbv12I GWGCWC 5 cut(s) 188, 231, 469, 627, 1911
BccI CCATC 3 cut(s) 122, 701, 1889
BceAI ACGGC 4 cut(s) 520, 810, 962, 1396
BcgI CGANNNNNNTGC 2 cut(s) 1406, 1440
BciT130I CCWGG 2 cut(s) 785, 1177
BciVI GTATCC 1 cut(s) 1534
BcnI CCSGG 8 cut(s) 296, 356, 357, 372, 1045, 1250, 1716, 1717
BcoDI GTCTC 4 cut(s) 86, 1371, 1872, 1970
BfaI CTAG 2 cut(s) 518, 1871
BfmI CTRYAG 3 cut(s) 51, 480, 849
BfoI RGCGCY 2 cut(s) 1015, 1699
BfuI GTATCC 1 cut(s) 1534
BglII AGATCT 1 cut(s) 1963
BlpI GCTNAGC 2 cut(s) 1773, 2022
Bme18I GGWCC 4 cut(s) 857, 1060, 1100, 1182
BmeRI GACNNNNNGTC 2 cut(s) 78, 241
BmeT110I CYCGRG 4 cut(s) 355, 1384, 1586, 1715
BmgBI CACGTC 1 cut(s) 565
BmgT120I GGNCC 8 cut(s) 857, 1060, 1100, 1182, 1708, 1709, 1837, 2031
BmrI ACTGGG 1 cut(s) 1285
BmsI GCATC 2 cut(s) 206, 1632
BmuI ACTGGG 1 cut(s) 1285
BoxI GACNNNNGTC 1 cut(s) 1079
BpiI GAAGAC 5 cut(s) 167, 549, 756, 783, 918
BplI GAGNNNNNCTC 2 cut(s) 791, 823
BpmI CTGGAG 1 cut(s) 427
Bpu10I CCTNAGC 1 cut(s) 2027
Bpu1102I GCTNAGC 2 cut(s) 1773, 2022
BpuMI CCSGG 8 cut(s) 296, 356, 357, 372, 1045, 1250, 1716, 1717
BsaAI YACGTR 3 cut(s) 400, 1555, 1595
BsaHI GRCGYC 5 cut(s) 939, 1374, 1406, 1450, 1865
BsaI GGTCTC 1 cut(s) 1970
BsaJI CCNNGG 9 cut(s) 34, 246, 294, 354, 355, 922, 1715, 1980, 2034
BsaWI WCCGGW 2 cut(s) 1273, 1828
BsaXI ACNNNNNCTCC 8 cut(s) 617, 647, 926, 956, 1789, 1819, 1950, 1980
Bsc4I CCNNNNNNNGG 9 cut(s) 107, 108, 362, 973, 1050, 1097, 1179, 1267, 1280
Bse118I RCCGGY 6 cut(s) 821, 1057, 1389, 1816, 1822, 1834
Bse1I ACTGG 3 cut(s) 415, 985, 1291
Bse21I CCTNAGG 1 cut(s) 1096
BseBI CCWGG 2 cut(s) 785, 1177
BseDI CCNNGG 9 cut(s) 34, 246, 294, 354, 355, 922, 1715, 1980, 2034
BseGI GGATG 4 cut(s) 117, 693, 915, 1286
BseLI CCNNNNNNNGG 9 cut(s) 107, 108, 362, 973, 1050, 1097, 1179, 1267, 1280
BseMII CTCAG 4 cut(s) 180, 300, 1110, 2013
BseNI ACTGG 3 cut(s) 415, 985, 1291
BseRI GAGGAG 2 cut(s) 206, 640
BseSI GKGCMC 2 cut(s) 1286, 1712
BseX3I CGGCCG 1 cut(s) 823
BseYI CCCAGC 1 cut(s) 723
Bsh1236I CGCG 4 cut(s) 248, 471, 1032, 1704
Bsh1285I CGRYCG 6 cut(s) 826, 835, 1278, 1548, 1652, 1770
BshFI GGCC 7 cut(s) 825, 977, 1054, 1526, 1710, 1838, 2033
BshNI GGYRCC 4 cut(s) 1498, 1819, 1825, 2009
BsiEI CGRYCG 6 cut(s) 826, 835, 1278, 1548, 1652, 1770
BsiHKAI GWGCWC 5 cut(s) 188, 231, 469, 627, 1911
BsiHKCI CYCGRG 4 cut(s) 355, 1384, 1586, 1715
BslFI GGGAC 6 cut(s) 84, 277, 870, 1361, 1417, 1876
BslI CCNNNNNNNGG 9 cut(s) 107, 108, 362, 973, 1050, 1097, 1179, 1267, 1280
BsmAI GTCTC 4 cut(s) 86, 1371, 1872, 1970
BsmBI CGTCTC 1 cut(s) 1872
BsmFI GGGAC 6 cut(s) 84, 277, 870, 1361, 1417, 1876
BsmI GAATGC 1 cut(s) 489
BsnI GGCC 7 cut(s) 825, 977, 1054, 1526, 1710, 1838, 2033
Bso31I GGTCTC 1 cut(s) 1970
BsoBI CYCGRG 4 cut(s) 355, 1384, 1586, 1715
Bsp120I GGGCCC 1 cut(s) 1708
Bsp1286I GDGCHC 8 cut(s) 188, 231, 469, 627, 1286, 1683, 1712, 1911
Bsp143I GATC 6 cut(s) 1545, 1582, 1615, 1628, 1767, 1963
Bsp1720I GCTNAGC 2 cut(s) 1773, 2022
BspANI GGCC 7 cut(s) 825, 977, 1054, 1526, 1710, 1838, 2033
BspCNI CTCAG 4 cut(s) 181, 301, 1109, 2014
BspFNI CGCG 4 cut(s) 248, 471, 1032, 1704
BspMAI CTGCAG 2 cut(s) 55, 853
BspPI GGATC 1 cut(s) 1590
BspT107I GGYRCC 4 cut(s) 1498, 1819, 1825, 2009
BspTNI GGTCTC 1 cut(s) 1970
BsrBI CCGCTC 2 cut(s) 1447, 1763
BsrFI RCCGGY 6 cut(s) 821, 1057, 1389, 1816, 1822, 1834
BsrI ACTGG 3 cut(s) 415, 985, 1291
BssAI RCCGGY 6 cut(s) 821, 1057, 1389, 1816, 1822, 1834
BssECI CCNNGG 9 cut(s) 34, 246, 294, 354, 355, 922, 1715, 1980, 2034
BssMI GATC 6 cut(s) 1545, 1582, 1615, 1628, 1767, 1963
BssNI GRCGYC 5 cut(s) 939, 1374, 1406, 1450, 1865
BssSI CACGAG 1 cut(s) 1624
BssT1I CCWWGG 1 cut(s) 2034
Bst2BI CACGAG 1 cut(s) 1624
Bst2UI CCWGG 2 cut(s) 785, 1177
Bst6I CTCTTC 2 cut(s) 207, 750
BstACI GRCGYC 5 cut(s) 939, 1374, 1406, 1450, 1865
BstBAI YACGTR 3 cut(s) 400, 1555, 1595
BstDEI CTNAG 9 cut(s) 189, 221, 309, 499, 1096, 1421, 1773, 2022, 2027
BstDSI CCRYGG 3 cut(s) 34, 246, 1980
BstENI CCTNNNNNAGG 1 cut(s) 1095
BstF5I GGATG 4 cut(s) 117, 693, 915, 1286
BstFNI CGCG 4 cut(s) 248, 471, 1032, 1704
BstH2I RGCGCY 2 cut(s) 1015, 1699
BstHHI GCGC 3 cut(s) 1014, 1034, 1698
BstKTI GATC 6 cut(s) 1548, 1585, 1618, 1631, 1770, 1966
BstMAI GTCTC 4 cut(s) 86, 1371, 1872, 1970
BstMBI GATC 6 cut(s) 1545, 1582, 1615, 1628, 1767, 1963
BstMCI CGRYCG 6 cut(s) 826, 835, 1278, 1548, 1652, 1770
BstNI CCWGG 2 cut(s) 785, 1177
BstNSI RCATGY 2 cut(s) 43, 973
BstPAI GACNNNNGTC 1 cut(s) 1079
BstSFI CTRYAG 3 cut(s) 51, 480, 849
BstSLI GKGCMC 2 cut(s) 1286, 1712
BstUI CGCG 4 cut(s) 248, 471, 1032, 1704
BstV2I GAAGAC 5 cut(s) 167, 549, 756, 783, 918
BstX2I RGATCY 2 cut(s) 1582, 1963
BstXI CCANNNNNNTGG 1 cut(s) 952
BstYI RGATCY 2 cut(s) 1582, 1963
BstZI CGGCCG 1 cut(s) 823
Bsu36I CCTNAGG 1 cut(s) 1096
BsuI GTATCC 1 cut(s) 1534
BsuRI GGCC 7 cut(s) 825, 977, 1054, 1526, 1710, 1838, 2033
BtgI CCRYGG 3 cut(s) 34, 246, 1980
BtgZI GCGATG 1 cut(s) 1908
BtrI CACGTC 1 cut(s) 565
BtsCI GGATG 4 cut(s) 117, 693, 915, 1286
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 5 cut(s) 60, 239, 408, 1158, 1298
CfoI GCGC 3 cut(s) 1014, 1034, 1698
Cfr10I RCCGGY 6 cut(s) 821, 1057, 1389, 1816, 1822, 1834
Cfr13I GGNCC 8 cut(s) 857, 1060, 1100, 1182, 1708, 1709, 1837, 2031
Cfr42I CCGCGG 1 cut(s) 249
Cfr9I CCCGGG 2 cut(s) 355, 1715
CseI GACGC 2 cut(s) 947, 1439
CsiI ACCWGGT 1 cut(s) 1175
Csp6I GTAC 4 cut(s) 1306, 1322, 1552, 1921
CviAII CATG 6 cut(s) 40, 970, 1433, 1660, 1852, 1948
CviQI GTAC 4 cut(s) 1306, 1322, 1552, 1921
DdeI CTNAG 9 cut(s) 189, 221, 309, 499, 1096, 1421, 1773, 2022, 2027
DpnI GATC 6 cut(s) 1547, 1584, 1617, 1630, 1769, 1965
DpnII GATC 6 cut(s) 1545, 1582, 1615, 1628, 1767, 1963
DraIII CACNNNGTG 1 cut(s) 1179
DrdI GACNNNNNNGTC 2 cut(s) 1189, 1864
DriI GACNNNNNGTC 2 cut(s) 78, 241
DseDI GACNNNNNNGTC 2 cut(s) 1189, 1864
EaeI YGGCCR 3 cut(s) 823, 975, 1052
EagI CGGCCG 1 cut(s) 823
Eam1104I CTCTTC 2 cut(s) 207, 750
Eam1105I GACNNNNNGTC 2 cut(s) 78, 241
EarI CTCTTC 2 cut(s) 207, 750
EciI GGCGGA 4 cut(s) 908, 979, 1773, 1797
Ecl136II GAGCTC 3 cut(s) 186, 229, 1909
EclXI CGGCCG 1 cut(s) 823
Eco130I CCWWGG 1 cut(s) 2034
Eco147I AGGCCT 1 cut(s) 1526
Eco24I GRGCYC 5 cut(s) 188, 231, 1683, 1712, 1911
Eco31I GGTCTC 1 cut(s) 1970
Eco47I GGWCC 4 cut(s) 857, 1060, 1100, 1182
Eco47III AGCGCT 1 cut(s) 1697
Eco52I CGGCCG 1 cut(s) 823
Eco53kI GAGCTC 3 cut(s) 186, 229, 1909
Eco81I CCTNAGG 1 cut(s) 1096
Eco88I CYCGRG 4 cut(s) 355, 1384, 1586, 1715
EcoICRI GAGCTC 3 cut(s) 186, 229, 1909
EcoNI CCTNNNNNAGG 1 cut(s) 1095
EcoO109I RGGNCCY 1 cut(s) 857
EcoRI GAATTC 1 cut(s) 1354
EcoRII CCWGG 2 cut(s) 783, 1175
EcoT14I CCWWGG 1 cut(s) 2034
EcoT38I GRGCYC 5 cut(s) 188, 231, 1683, 1712, 1911
ErhI CCWWGG 1 cut(s) 2034
Esp3I CGTCTC 1 cut(s) 1872
FaeI CATG 6 cut(s) 43, 973, 1436, 1663, 1855, 1951
FaqI GGGAC 6 cut(s) 84, 277, 870, 1361, 1417, 1876
FatI CATG 6 cut(s) 39, 969, 1432, 1659, 1851, 1947
FblI GTMKAC 4 cut(s) 582, 1040, 1157, 1377
FokI GGATG 4 cut(s) 124, 680, 902, 1273
FriOI GRGCYC 5 cut(s) 188, 231, 1683, 1712, 1911
FspBI CTAG 2 cut(s) 518, 1871
GlaI GCGC 3 cut(s) 1013, 1033, 1697
GsaI CCCAGC 1 cut(s) 727
GsuI CTGGAG 1 cut(s) 427
HaeII RGCGCY 2 cut(s) 1015, 1699
HaeIII GGCC 7 cut(s) 825, 977, 1054, 1526, 1710, 1838, 2033
HgaI GACGC 2 cut(s) 947, 1439
HhaI GCGC 3 cut(s) 1014, 1034, 1698
Hin1I GRCGYC 5 cut(s) 939, 1374, 1406, 1450, 1865
Hin1II CATG 6 cut(s) 43, 973, 1436, 1663, 1855, 1951
Hin6I GCGC 3 cut(s) 1012, 1032, 1696
HinP1I GCGC 3 cut(s) 1012, 1032, 1696
HincII GTYRAC 5 cut(s) 583, 1041, 1158, 1378, 1857
HindII GTYRAC 5 cut(s) 583, 1041, 1158, 1378, 1857
HindIII AAGCTT 1 cut(s) 1463
HinfI GANTC 9 cut(s) 236, 311, 800, 806, 956, 1022, 1037, 1187, 1576
HphI GGTGA 3 cut(s) 679, 1201, 1699
Hpy166II GTNNAC 8 cut(s) 583, 1041, 1158, 1182, 1242, 1322, 1378, 1857
Hpy188III TCNNGA 9 cut(s) 151, 444, 539, 1098, 1346, 1370, 1580, 1586, 1871
Hpy8I GTNNAC 8 cut(s) 583, 1041, 1158, 1182, 1242, 1322, 1378, 1857
Hpy99I CGWCG 9 cut(s) 584, 938, 941, 1133, 1195, 1376, 1379, 1382, 1455
HpyAV CCTTC 7 cut(s) 448, 562, 605, 788, 1393, 1922, 2023
HpyF3I CTNAG 9 cut(s) 189, 221, 309, 499, 1096, 1421, 1773, 2022, 2027
Hsp92I GRCGYC 5 cut(s) 939, 1374, 1406, 1450, 1865
Hsp92II CATG 6 cut(s) 43, 973, 1436, 1663, 1855, 1951
HspAI GCGC 3 cut(s) 1012, 1032, 1696
KflI GGGWCCC 1 cut(s) 857
KspI CCGCGG 1 cut(s) 249
Kzo9I GATC 6 cut(s) 1545, 1582, 1615, 1628, 1767, 1963
LweI GCATC 2 cut(s) 206, 1632
MabI ACCWGGT 1 cut(s) 1175
MaeI CTAG 2 cut(s) 518, 1871
MaeIII GTNAC 8 cut(s) 42, 827, 1207, 1407, 1507, 1595, 1687, 1935
MalI GATC 6 cut(s) 1547, 1584, 1617, 1630, 1769, 1965
MbiI CCGCTC 2 cut(s) 1447, 1763
MboI GATC 6 cut(s) 1545, 1582, 1615, 1628, 1767, 1963
MflI RGATCY 2 cut(s) 1582, 1963
MhlI GDGCHC 8 cut(s) 188, 231, 469, 627, 1286, 1683, 1712, 1911
MluCI AATT 5 cut(s) 949, 1118, 1354, 1971, 2045
MlyI GAGTC 7 cut(s) 230, 320, 800, 809, 950, 1016, 1046
MseI TTAA 5 cut(s) 116, 305, 1467, 2066, 2083
MslI CAYNNNNRTG 2 cut(s) 1291, 1985
MspA1I CMGCKG 4 cut(s) 248, 854, 1079, 1105
Mva1269I GAATGC 1 cut(s) 489
MvaI CCWGG 2 cut(s) 785, 1177
MvnI CGCG 4 cut(s) 248, 471, 1032, 1704
NciI CCSGG 8 cut(s) 296, 356, 357, 372, 1045, 1250, 1716, 1717
NdeII GATC 6 cut(s) 1545, 1582, 1615, 1628, 1767, 1963
NlaIII CATG 6 cut(s) 43, 973, 1436, 1663, 1855, 1951
NmeAIII GCCGAG 4 cut(s) 901, 903, 906, 1060
NmuCI GTSAC 4 cut(s) 1207, 1407, 1507, 1687
NspI RCATGY 2 cut(s) 43, 973
OliI CACNNNNGTG 1 cut(s) 1985
PaeR7I CTCGAG 2 cut(s) 1384, 1586
PceI AGGCCT 1 cut(s) 1526
PcsI WCGNNNNNNNCGW 6 cut(s) 561, 759, 786, 906, 930, 1966
PctI GAATGC 1 cut(s) 489
PfeI GAWTC 2 cut(s) 1187, 1576
PflFI GACNNNGTC 1 cut(s) 1205
PflMI CCANNNNNTGG 3 cut(s) 973, 1179, 1267
Ple19I CGATCG 2 cut(s) 1548, 1770
PleI GAGTC 7 cut(s) 230, 319, 800, 808, 950, 1016, 1045
PpsI GAGTC 7 cut(s) 230, 319, 800, 808, 950, 1016, 1045
Ppu21I YACGTR 3 cut(s) 400, 1555, 1595
PpuMI RGGWCCY 1 cut(s) 857
PshAI GACNNNNGTC 1 cut(s) 1079
Psp124BI GAGCTC 3 cut(s) 188, 231, 1911
Psp1406I AACGTT 1 cut(s) 864
Psp5II RGGWCCY 1 cut(s) 857
Psp6I CCWGG 2 cut(s) 783, 1175
PspFI CCCAGC 1 cut(s) 723
PspGI CCWGG 2 cut(s) 783, 1175
PspOMI GGGCCC 1 cut(s) 1708
PspPI GGNCC 8 cut(s) 857, 1060, 1100, 1182, 1708, 1709, 1837, 2031
PspPPI RGGWCCY 1 cut(s) 857
PspXI VCTCGAGB 1 cut(s) 1384
PstI CTGCAG 2 cut(s) 55, 853
PsuI RGATCY 2 cut(s) 1582, 1963
PsyI GACNNNGTC 1 cut(s) 1205
PvuI CGATCG 2 cut(s) 1548, 1770
RgaI GCGATCGC 1 cut(s) 1770
RsaI GTAC 4 cut(s) 1307, 1323, 1553, 1922
RsaNI GTAC 4 cut(s) 1306, 1322, 1552, 1921
RseI CAYNNNNRTG 2 cut(s) 1291, 1985
SacI GAGCTC 3 cut(s) 188, 231, 1911
SacII CCGCGG 1 cut(s) 249
SalI GTCGAC 4 cut(s) 581, 1039, 1156, 1376
SaqAI TTAA 5 cut(s) 116, 305, 1467, 2066, 2083
Sau3AI GATC 6 cut(s) 1545, 1582, 1615, 1628, 1767, 1963
Sau96I GGNCC 8 cut(s) 857, 1060, 1100, 1182, 1708, 1709, 1837, 2031
SchI GAGTC 7 cut(s) 230, 320, 800, 809, 950, 1016, 1046
SduI GDGCHC 8 cut(s) 188, 231, 469, 627, 1286, 1683, 1712, 1911
SexAI ACCWGGT 1 cut(s) 1175
SfaAI GCGATCGC 1 cut(s) 1770
SfaNI GCATC 2 cut(s) 206, 1632
SfcI CTRYAG 3 cut(s) 51, 480, 849
Sfr274I CTCGAG 2 cut(s) 1384, 1586
Sfr303I CCGCGG 1 cut(s) 249
SgfI GCGATCGC 1 cut(s) 1770
SgrBI CCGCGG 1 cut(s) 249
SgrDI CGTCGACG 1 cut(s) 1376
SinI GGWCC 4 cut(s) 857, 1060, 1100, 1182
SlaI CTCGAG 2 cut(s) 1384, 1586
SmaI CCCGGG 2 cut(s) 357, 1717
SmiMI CAYNNNNRTG 2 cut(s) 1291, 1985
SmlI CTYRAG 2 cut(s) 1384, 1586
SmoI CTYRAG 2 cut(s) 1384, 1586
Sse9I AATT 5 cut(s) 949, 1118, 1354, 1971, 2045
SseBI AGGCCT 1 cut(s) 1526
SspMI CTAG 2 cut(s) 518, 1871
SstI GAGCTC 3 cut(s) 188, 231, 1911
StuI AGGCCT 1 cut(s) 1526
StyI CCWWGG 1 cut(s) 2034
TaqII GACCGA 1 cut(s) 1199
TasI AATT 5 cut(s) 949, 1118, 1354, 1971, 2045
TatI WGTACW 1 cut(s) 1305
TauI GCSGC 7 cut(s) 276, 713, 743, 1001, 1057, 1450, 1766
TfiI GAWTC 2 cut(s) 1187, 1576
Tru1I TTAA 5 cut(s) 116, 305, 1467, 2066, 2083
Tru9I TTAA 5 cut(s) 116, 305, 1467, 2066, 2083
TscAI CASTG 5 cut(s) 60, 246, 415, 1158, 1298
TseFI GTSAC 4 cut(s) 1207, 1407, 1507, 1687
Tsp45I GTSAC 4 cut(s) 1207, 1407, 1507, 1687
TspDTI ATGAA 2 cut(s) 435, 1676
TspGWI ACGGA 2 cut(s) 23, 1706
TspMI CCCGGG 2 cut(s) 355, 1715
TspRI CASTG 5 cut(s) 60, 246, 415, 1158, 1298
Tth111I GACNNNGTC 1 cut(s) 1205
Van91I CCANNNNNTGG 3 cut(s) 973, 1179, 1267
VpaK11BI GGWCC 4 cut(s) 857, 1060, 1100, 1182
XagI CCTNNNNNAGG 1 cut(s) 1095
XapI RAATTY 1 cut(s) 1354
XbaI TCTAGA 1 cut(s) 1870
XceI RCATGY 2 cut(s) 43, 973
XhoI CTCGAG 2 cut(s) 1384, 1586
XmaI CCCGGG 2 cut(s) 355, 1715
XmiI GTMKAC 4 cut(s) 582, 1040, 1157, 1377
XspI CTAG 2 cut(s) 518, 1871
ZraI GACGTC 3 cut(s) 1375, 1407, 1866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.