RLG00000012139
BHLH Family

basic helix-loop-helix protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010418
Physical Location & Seq
Forward (+)
15678154 .. 15682907
4754 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012139

Sequence Viewer

Length: 2100 bp
ATGGCTACACCGCCACCGAGTAGTAGCAGGCTCCGTGGCATGTTACAGTCTGCAGTGCAATCTGTTCAATGGACTTACAGTCTCTTTTGGCAAATCTGTCCCCAACAAGGGATGTTAATATGGGCAGATGGGTATTACAATGGGGCAATCAAGACGAGGAAGACTGTGCAACCTATGGAAGTGAGCTCTGAGGAGGCATCTCTTCAAAGAAGCCAACAGCTTAGAGAGCTCTACGACTCACTGTCCGCCGGAGAAACAAACCAGCCAGCTCGCCGCCCTTGTGCTGCCTTGTCCCCGGAGGACTTAACCGAGTCGGAATGGTTCTACTTGATGTGTGTATCTTTCTCATTTCCCCCGGGAGTTGGGTTGCCGGGAAAAGCATACACAAGGAGGCAACACGTATGGCTCACTGGTGCAAATGAAGTAGATAGCAAAACCTTCTCCAGAGCTATTTTAGCCAAGAGTGCTCGCGTTCAAACTGTAGTGTGCATTCCTCTCTTAGACGGCGTCGTTGAGCTAGGCACAACAGATAGGGTTCCAGAAGACCTTGCCTTCGTCCAACACGTCAAAACTTTCTTCGTCGACCACCACCACCTTCCACCACCAAAGCCCGCCCTCTCGGAGCACTCCACCTCCAATCCCGCCACCTCCTCCGACCACCACCCCCGTTTCCACTCTCCACATCTCACCGCCATCCGCAACAACGCTGCCGCAAACAACCCCCCGCCCCTCCACGCAGCCGCCCAAGAAGACGAAGAGGACGAGGACGAGGAAGAAGACGACCAGGACGAAGAAGGAGAGTCCGACTCCGAAGCCGAAACCGGCCGTAACGGTCGTCCTCTCTCTGCTGCTGCGGGACCCAACGTTCCAGCTGCCGAGCCGAGCGAGCTCATGCAGCTCGAAATGTCCGAAGACATCCGCCTCGGCTCCCCCGACGACGCCTCCAACAACTTGGACTCGGACTTCCACATGCTGGCCGTCAGCCAGTCCGCCAATCAGCAGCGCCAAGCAGACTCGTATCGCGCAGAGTCGACCCGGCGGTGGCCGCCTGTCCAACCCCCAATGACCGCTGTCCAACCACCTCCCTCAGGACCGCTGGCATTGGAGGAATTGACCCACGACGACACACACTACTCGGAAACAGTGTCGACCATACTTCAAACCCAAGCTACTCGGTGGACCGATTCGTCGTCCAATGACTACGTCACCTACTCCACCCAATCAGCTTTCGCCAAGTGGATGAGCCGGGCTGACCACCACCTCTTGGTTCCGGTCGAGGGCACATCCCAGTGGCTCCTGAAGTACATTTTGTTTAGTGTACCGTTCCTACATACGAAATATCGGGACGAGAATTCCCCCAAGTCTCACGACGTCGACGGCTCCAGCCGGTTGAGGAAGGGGACGTCACAAGACGAGCTAAGTGCTAACCATGTGTTAGCGGAGCGGCGTCGTAGAGAGAAGCTTAATGAGAGGTTTATTATATTGAGGTCTTTGGTGCCTTTTGTGACAAAAATGGACAAGGCCTCGATTTTGGGGGACACGATCGAGTACGTGAAGCAACTGCGTAAGAAGATTCAGGATCTCGAGGCACGTAACGTGCATTTGGAGGACGATCAACGGACGAGATCGGCGGGGGAGATGCAACGGTCGAGTAGCATGAAAGAGTTGCGGAGCGGGCTCACGGTGACGGAGCGCTCCCGCGTGGGCCCGCCCGGGTCGGACAAGAGAAAGTTAAGGATTGTGGAGGGGAGTGGCGGAGCAGCGATCGCTAAACCTAAAGTGATAGAGGAACCGACACCACCTCCACCACCACCGGCACCAGAACCGGCCCCGCCAACGCCCATGTTGACGGGAACGTCTCTAGAGGTGTCGATAATAGAGAGCGATGGATTGTTGGAGCTCCAATGCCCGTACAGAGAAGGGTTGTTACTTGATGTCATGCAAACCCTACGAGATCTCCGAATTGAGACCACGGTTGTGCAATCCTCGTTGAATAGTGGCACCTTCGTAGCTGAGCTAAGGGCCAAGGTGGGCAAGAAAACAACCATTACAGAAGTGAAGAGGGCAGTAAATCAAGTGATACCCCAATCTGACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH bHLH-MYC_N

Protein Analysis

700

Amino Acids

77.56

Weight (kDa)

5.45

Isoelectric Point (pI)

65.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 11 - 193 3.8e-53 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 475 - 522 7e-13 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 621 - 694 3.1e-15 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1186, 1855
AatII GACGTC 2 cut(s) 1374, 1406
AccB1I GGYRCC 3 cut(s) 1495, 1816, 2000
AccB7I CCANNNNNTGG 2 cut(s) 973, 1264
AccBSI CCGCTC 2 cut(s) 1444, 1674
AccI GTMKAC 4 cut(s) 582, 1031, 1148, 1374
AccII CGCG 3 cut(s) 471, 1023, 1701
AclI AACGTT 1 cut(s) 864
AclWI GGATC 1 cut(s) 1587
AcoI YGGCCR 3 cut(s) 823, 975, 1043
AcsI RAATTY 1 cut(s) 1351
AcuI CTGAAG 1 cut(s) 1319
AcyI GRCGYC 5 cut(s) 507, 939, 1371, 1403, 1447
AfaI GTAC 4 cut(s) 1304, 1320, 1550, 1913
AfeI AGCGCT 1 cut(s) 1694
AfiI CCNNNNNNNGG 8 cut(s) 107, 108, 362, 973, 1041, 1088, 1264, 1277
AflIII ACRYGT 2 cut(s) 397, 562
AgsI TTSAA 5 cut(s) 68, 206, 476, 1160, 1993
AhdI GACNNNNNGTC 2 cut(s) 78, 241
AjiI CACGTC 1 cut(s) 565
AjnI CCWGG 1 cut(s) 783
AleI CACNNNNGTG 1 cut(s) 1976
AloI GAACNNNNNNTCC 2 cut(s) 849, 881
Alw21I GWGCWC 6 cut(s) 188, 231, 469, 627, 891, 1902
Alw26I GTCTC 4 cut(s) 86, 1368, 1863, 1961
AlwI GGATC 1 cut(s) 1587
Ama87I CYCGRG 3 cut(s) 355, 1583, 1712
Aor51HI AGCGCT 1 cut(s) 1694
AoxI GGCC 7 cut(s) 823, 975, 1043, 1521, 1705, 1827, 2022
ApaI GGGCCC 1 cut(s) 1709
ApeKI GCWGC 9 cut(s) 284, 707, 737, 848, 851, 872, 895, 1000, 1760
ApoI RAATTY 1 cut(s) 1351
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
AsiSI GCGATCGC 1 cut(s) 1767
AspLEI GCGC 3 cut(s) 1005, 1025, 1695
AspS9I GGNCC 7 cut(s) 857, 1091, 1179, 1705, 1706, 1828, 2022
AsuC2I CCSGG 8 cut(s) 296, 356, 357, 372, 1036, 1247, 1713, 1714
AsuHPI GGTGA 3 cut(s) 679, 1198, 1696
AvaI CYCGRG 3 cut(s) 355, 1583, 1712
AvaII GGWCC 3 cut(s) 857, 1091, 1179
AxyI CCTNAGG 1 cut(s) 1087
BaeGI GKGCMC 2 cut(s) 1283, 1709
BanI GGYRCC 3 cut(s) 1495, 1816, 2000
BanII GRGCYC 6 cut(s) 188, 231, 891, 1680, 1709, 1902
BarI GAAGNNNNNNTAC 2 cut(s) 1911, 1943
BbsI GAAGAC 5 cut(s) 167, 549, 756, 783, 918
Bbv12I GWGCWC 6 cut(s) 188, 231, 469, 627, 891, 1902
BbvI GCAGC 9 cut(s) 271, 694, 749, 835, 838, 859, 907, 1012, 1772
BccI CCATC 3 cut(s) 122, 701, 1880
BceAI ACGGC 4 cut(s) 520, 810, 962, 1393
BcgI CGANNNNNNTGC 2 cut(s) 1403, 1437
BciT130I CCWGG 1 cut(s) 785
BcnI CCSGG 8 cut(s) 296, 356, 357, 372, 1036, 1247, 1713, 1714
BcoDI GTCTC 4 cut(s) 86, 1368, 1863, 1961
BfaI CTAG 2 cut(s) 518, 1862
BfmI CTRYAG 2 cut(s) 51, 480
BfoI RGCGCY 2 cut(s) 1006, 1696
BglII AGATCT 1 cut(s) 1954
BlpI GCTNAGC 1 cut(s) 2013
Bme1390I CCNGG 9 cut(s) 296, 356, 357, 372, 785, 1036, 1247, 1713, 1714
Bme18I GGWCC 3 cut(s) 857, 1091, 1179
BmeRI GACNNNNNGTC 2 cut(s) 78, 241
BmeT110I CYCGRG 3 cut(s) 355, 1583, 1712
BmgBI CACGTC 1 cut(s) 565
BmgT120I GGNCC 7 cut(s) 857, 1091, 1179, 1705, 1706, 1828, 2022
BmrFI CCNGG 9 cut(s) 296, 356, 357, 372, 785, 1036, 1247, 1713, 1714
BmrI ACTGGG 1 cut(s) 1282
BmsI GCATC 2 cut(s) 206, 1629
BmuI ACTGGG 1 cut(s) 1282
BoxI GACNNNNGTC 1 cut(s) 1070
BpiI GAAGAC 5 cut(s) 167, 549, 756, 783, 918
BplI GAGNNNNNCTC 2 cut(s) 791, 823
BpmI CTGGAG 2 cut(s) 427, 1366
Bpu10I CCTNAGC 1 cut(s) 2018
Bpu1102I GCTNAGC 1 cut(s) 2013
BpuMI CCSGG 8 cut(s) 296, 356, 357, 372, 1036, 1247, 1713, 1714
BsaAI YACGTR 3 cut(s) 400, 1552, 1592
BsaHI GRCGYC 5 cut(s) 507, 939, 1371, 1403, 1447
BsaI GGTCTC 1 cut(s) 1961
BsaJI CCNNGG 8 cut(s) 34, 294, 354, 355, 922, 1712, 1971, 2025
BsaWI WCCGGW 1 cut(s) 1270
BsaXI ACNNNNNCTCC 8 cut(s) 617, 647, 926, 956, 1786, 1816, 1941, 1971
Bsc4I CCNNNNNNNGG 8 cut(s) 107, 108, 362, 973, 1041, 1088, 1264, 1277
Bse118I RCCGGY 4 cut(s) 821, 1386, 1813, 1825
Bse1I ACTGG 3 cut(s) 415, 985, 1288
Bse21I CCTNAGG 1 cut(s) 1087
BseBI CCWGG 1 cut(s) 785
BseDI CCNNGG 8 cut(s) 34, 294, 354, 355, 922, 1712, 1971, 2025
BseGI GGATG 5 cut(s) 117, 693, 915, 1245, 1283
BseLI CCNNNNNNNGG 8 cut(s) 107, 108, 362, 973, 1041, 1088, 1264, 1277
BseMII CTCAG 3 cut(s) 180, 1101, 2004
BseNI ACTGG 3 cut(s) 415, 985, 1288
BseRI GAGGAG 2 cut(s) 206, 640
BseSI GKGCMC 2 cut(s) 1283, 1709
BseX3I CGGCCG 1 cut(s) 823
BseXI GCAGC 9 cut(s) 271, 694, 749, 835, 838, 859, 907, 1012, 1772
Bsh1236I CGCG 3 cut(s) 471, 1023, 1701
Bsh1285I CGRYCG 6 cut(s) 826, 835, 1275, 1545, 1649, 1767
BshFI GGCC 7 cut(s) 825, 977, 1045, 1523, 1707, 1829, 2024
BshNI GGYRCC 3 cut(s) 1495, 1816, 2000
BsiEI CGRYCG 6 cut(s) 826, 835, 1275, 1545, 1649, 1767
BsiHKAI GWGCWC 6 cut(s) 188, 231, 469, 627, 891, 1902
BsiHKCI CYCGRG 3 cut(s) 355, 1583, 1712
BslFI GGGAC 6 cut(s) 84, 277, 870, 1358, 1414, 1550
BslI CCNNNNNNNGG 8 cut(s) 107, 108, 362, 973, 1041, 1088, 1264, 1277
BsmAI GTCTC 4 cut(s) 86, 1368, 1863, 1961
BsmBI CGTCTC 1 cut(s) 1863
BsmFI GGGAC 6 cut(s) 84, 277, 870, 1358, 1414, 1550
BsmI GAATGC 1 cut(s) 489
BsnI GGCC 7 cut(s) 825, 977, 1045, 1523, 1707, 1829, 2024
Bso31I GGTCTC 1 cut(s) 1961
BsoBI CYCGRG 3 cut(s) 355, 1583, 1712
Bsp120I GGGCCC 1 cut(s) 1705
Bsp1286I GDGCHC 9 cut(s) 188, 231, 469, 627, 891, 1283, 1680, 1709, 1902
Bsp143I GATC 6 cut(s) 1542, 1579, 1612, 1625, 1764, 1954
Bsp1720I GCTNAGC 1 cut(s) 2013
BspANI GGCC 7 cut(s) 825, 977, 1045, 1523, 1707, 1829, 2024
BspCNI CTCAG 3 cut(s) 181, 1100, 2005
BspFNI CGCG 3 cut(s) 471, 1023, 1701
BspMAI CTGCAG 1 cut(s) 55
BspPI GGATC 1 cut(s) 1587
BspT107I GGYRCC 3 cut(s) 1495, 1816, 2000
BspTNI GGTCTC 1 cut(s) 1961
BsrBI CCGCTC 2 cut(s) 1444, 1674
BsrFI RCCGGY 4 cut(s) 821, 1386, 1813, 1825
BsrI ACTGG 3 cut(s) 415, 985, 1288
BssAI RCCGGY 4 cut(s) 821, 1386, 1813, 1825
BssECI CCNNGG 8 cut(s) 34, 294, 354, 355, 922, 1712, 1971, 2025
BssMI GATC 6 cut(s) 1542, 1579, 1612, 1625, 1764, 1954
BssNI GRCGYC 5 cut(s) 507, 939, 1371, 1403, 1447
BssT1I CCWWGG 1 cut(s) 2025
Bst2UI CCWGG 1 cut(s) 785
Bst6I CTCTTC 3 cut(s) 207, 750, 2054
BstACI GRCGYC 5 cut(s) 507, 939, 1371, 1403, 1447
BstBAI YACGTR 3 cut(s) 400, 1552, 1592
BstDEI CTNAG 7 cut(s) 189, 221, 499, 1087, 1418, 2013, 2018
BstDSI CCRYGG 2 cut(s) 34, 1971
BstENI CCTNNNNNAGG 1 cut(s) 1086
BstF5I GGATG 5 cut(s) 117, 693, 915, 1245, 1283
BstFNI CGCG 3 cut(s) 471, 1023, 1701
BstH2I RGCGCY 2 cut(s) 1006, 1696
BstHHI GCGC 3 cut(s) 1005, 1025, 1695
BstKTI GATC 6 cut(s) 1545, 1582, 1615, 1628, 1767, 1957
BstMAI GTCTC 4 cut(s) 86, 1368, 1863, 1961
BstMBI GATC 6 cut(s) 1542, 1579, 1612, 1625, 1764, 1954
BstMCI CGRYCG 6 cut(s) 826, 835, 1275, 1545, 1649, 1767
BstMWI GCNNNNNNNGC 8 cut(s) 226, 455, 464, 886, 895, 1045, 1675, 1766
BstNI CCWGG 1 cut(s) 785
BstNSI RCATGY 2 cut(s) 43, 973
BstPAI GACNNNNGTC 1 cut(s) 1070
BstSCI CCNGG 9 cut(s) 294, 354, 355, 370, 783, 1034, 1245, 1711, 1712
BstSFI CTRYAG 2 cut(s) 51, 480
BstSLI GKGCMC 2 cut(s) 1283, 1709
BstUI CGCG 3 cut(s) 471, 1023, 1701
BstV1I GCAGC 9 cut(s) 271, 694, 749, 835, 838, 859, 907, 1012, 1772
BstV2I GAAGAC 5 cut(s) 167, 549, 756, 783, 918
BstX2I RGATCY 2 cut(s) 1579, 1954
BstXI CCANNNNNNTGG 1 cut(s) 952
BstYI RGATCY 2 cut(s) 1579, 1954
BstZI CGGCCG 1 cut(s) 823
Bsu36I CCTNAGG 1 cut(s) 1087
BsuRI GGCC 7 cut(s) 825, 977, 1045, 1523, 1707, 1829, 2024
BtgI CCRYGG 2 cut(s) 34, 1971
BtgZI GCGATG 1 cut(s) 1899
BtrI CACGTC 1 cut(s) 565
BtsCI GGATG 5 cut(s) 117, 693, 915, 1245, 1283
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 5 cut(s) 60, 239, 408, 1149, 1295
CfoI GCGC 3 cut(s) 1005, 1025, 1695
Cfr10I RCCGGY 4 cut(s) 821, 1386, 1813, 1825
Cfr13I GGNCC 7 cut(s) 857, 1091, 1179, 1705, 1706, 1828, 2022
Cfr9I CCCGGG 2 cut(s) 355, 1712
CseI GACGC 3 cut(s) 496, 947, 1436
Csp6I GTAC 4 cut(s) 1303, 1319, 1549, 1912
CviAII CATG 7 cut(s) 40, 892, 970, 1430, 1657, 1843, 1939
CviQI GTAC 4 cut(s) 1303, 1319, 1549, 1912
DdeI CTNAG 7 cut(s) 189, 221, 499, 1087, 1418, 2013, 2018
DpnI GATC 6 cut(s) 1544, 1581, 1614, 1627, 1766, 1956
DpnII GATC 6 cut(s) 1542, 1579, 1612, 1625, 1764, 1954
DrdI GACNNNNNNGTC 2 cut(s) 1186, 1855
DriI GACNNNNNGTC 2 cut(s) 78, 241
DseDI GACNNNNNNGTC 2 cut(s) 1186, 1855
EaeI YGGCCR 3 cut(s) 823, 975, 1043
EagI CGGCCG 1 cut(s) 823
Eam1104I CTCTTC 3 cut(s) 207, 750, 2054
Eam1105I GACNNNNNGTC 2 cut(s) 78, 241
EarI CTCTTC 3 cut(s) 207, 750, 2054
EciI GGCGGA 4 cut(s) 235, 908, 979, 1770
Ecl136II GAGCTC 4 cut(s) 186, 229, 889, 1900
EclXI CGGCCG 1 cut(s) 823
Eco130I CCWWGG 1 cut(s) 2025
Eco147I AGGCCT 1 cut(s) 1523
Eco24I GRGCYC 6 cut(s) 188, 231, 891, 1680, 1709, 1902
Eco31I GGTCTC 1 cut(s) 1961
Eco47I GGWCC 3 cut(s) 857, 1091, 1179
Eco47III AGCGCT 1 cut(s) 1694
Eco52I CGGCCG 1 cut(s) 823
Eco53kI GAGCTC 4 cut(s) 186, 229, 889, 1900
Eco57I CTGAAG 1 cut(s) 1319
Eco81I CCTNAGG 1 cut(s) 1087
Eco88I CYCGRG 3 cut(s) 355, 1583, 1712
EcoICRI GAGCTC 4 cut(s) 186, 229, 889, 1900
EcoNI CCTNNNNNAGG 1 cut(s) 1086
EcoO109I RGGNCCY 1 cut(s) 857
EcoRI GAATTC 1 cut(s) 1351
EcoRII CCWGG 1 cut(s) 783
EcoT14I CCWWGG 1 cut(s) 2025
EcoT38I GRGCYC 6 cut(s) 188, 231, 891, 1680, 1709, 1902
ErhI CCWWGG 1 cut(s) 2025
Esp3I CGTCTC 1 cut(s) 1863
FaeI CATG 7 cut(s) 43, 895, 973, 1433, 1660, 1846, 1942
FaqI GGGAC 6 cut(s) 84, 277, 870, 1358, 1414, 1550
FatI CATG 7 cut(s) 39, 891, 969, 1429, 1656, 1842, 1938
FauI CCCGC 9 cut(s) 619, 649, 732, 847, 1624, 1667, 1706, 1716, 1839
FblI GTMKAC 4 cut(s) 582, 1031, 1148, 1374
FokI GGATG 5 cut(s) 124, 680, 902, 1252, 1270
FriOI GRGCYC 6 cut(s) 188, 231, 891, 1680, 1709, 1902
FspBI CTAG 2 cut(s) 518, 1862
GlaI GCGC 3 cut(s) 1004, 1024, 1694
GsuI CTGGAG 2 cut(s) 427, 1366
HaeII RGCGCY 2 cut(s) 1006, 1696
HaeIII GGCC 7 cut(s) 825, 977, 1045, 1523, 1707, 1829, 2024
HgaI GACGC 3 cut(s) 496, 947, 1436
HhaI GCGC 3 cut(s) 1005, 1025, 1695
Hin1I GRCGYC 5 cut(s) 507, 939, 1371, 1403, 1447
Hin1II CATG 7 cut(s) 43, 895, 973, 1433, 1660, 1846, 1942
Hin6I GCGC 3 cut(s) 1003, 1023, 1693
HinP1I GCGC 3 cut(s) 1003, 1023, 1693
HincII GTYRAC 5 cut(s) 583, 1032, 1149, 1375, 1848
HindII GTYRAC 5 cut(s) 583, 1032, 1149, 1375, 1848
HindIII AAGCTT 1 cut(s) 1460
HphI GGTGA 3 cut(s) 679, 1198, 1696
Hpy166II GTNNAC 7 cut(s) 583, 1032, 1149, 1179, 1319, 1375, 1848
Hpy8I GTNNAC 7 cut(s) 583, 1032, 1149, 1179, 1319, 1375, 1848
HpyAV CCTTC 7 cut(s) 448, 562, 605, 788, 1390, 1913, 2014
HpyF10VI GCNNNNNNNGC 8 cut(s) 226, 455, 464, 886, 895, 1045, 1675, 1766
HpyF3I CTNAG 7 cut(s) 189, 221, 499, 1087, 1418, 2013, 2018
Hsp92I GRCGYC 5 cut(s) 507, 939, 1371, 1403, 1447
Hsp92II CATG 7 cut(s) 43, 895, 973, 1433, 1660, 1846, 1942
HspAI GCGC 3 cut(s) 1003, 1023, 1693
KflI GGGWCCC 1 cut(s) 857
Kzo9I GATC 6 cut(s) 1542, 1579, 1612, 1625, 1764, 1954
Lsp1109I GCAGC 9 cut(s) 271, 694, 749, 835, 838, 859, 907, 1012, 1772
LweI GCATC 2 cut(s) 206, 1629
MaeI CTAG 2 cut(s) 518, 1862
MaeIII GTNAC 8 cut(s) 42, 827, 1204, 1404, 1504, 1592, 1684, 1926
MalI GATC 6 cut(s) 1544, 1581, 1614, 1627, 1766, 1956
MbiI CCGCTC 2 cut(s) 1444, 1674
MboI GATC 6 cut(s) 1542, 1579, 1612, 1625, 1764, 1954
MflI RGATCY 2 cut(s) 1579, 1954
MhlI GDGCHC 9 cut(s) 188, 231, 469, 627, 891, 1283, 1680, 1709, 1902
MluCI AATT 3 cut(s) 1109, 1351, 1962
MlyI GAGTC 8 cut(s) 230, 320, 800, 809, 950, 1007, 1037, 2087
MmeI TCCRAC 9 cut(s) 294, 583, 678, 828, 969, 1078, 1099, 1698, 1875
MseI TTAA 5 cut(s) 116, 305, 1464, 1733, 2098
MslI CAYNNNNRTG 2 cut(s) 1288, 1976
MspA1I CMGCKG 3 cut(s) 872, 1070, 1096
MspR9I CCNGG 9 cut(s) 296, 356, 357, 372, 785, 1036, 1247, 1713, 1714
Mva1269I GAATGC 1 cut(s) 489
MvaI CCWGG 1 cut(s) 785
MvnI CGCG 3 cut(s) 471, 1023, 1701
MwoI GCNNNNNNNGC 8 cut(s) 226, 455, 464, 886, 895, 1045, 1675, 1766
NciI CCSGG 8 cut(s) 296, 356, 357, 372, 1036, 1247, 1713, 1714
NdeII GATC 6 cut(s) 1542, 1579, 1612, 1625, 1764, 1954
NlaIII CATG 7 cut(s) 43, 895, 973, 1433, 1660, 1846, 1942
NmeAIII GCCGAG 3 cut(s) 901, 903, 906
NmuCI GTSAC 4 cut(s) 1204, 1404, 1504, 1684
NspI RCATGY 2 cut(s) 43, 973
OliI CACNNNNGTG 1 cut(s) 1976
PaeR7I CTCGAG 1 cut(s) 1583
PceI AGGCCT 1 cut(s) 1523
PcsI WCGNNNNNNNCGW 6 cut(s) 561, 759, 786, 906, 930, 1957
PctI GAATGC 1 cut(s) 489
PfeI GAWTC 2 cut(s) 1184, 1573
PflFI GACNNNGTC 2 cut(s) 506, 1202
PflMI CCANNNNNTGG 2 cut(s) 973, 1264
Ple19I CGATCG 2 cut(s) 1545, 1767
PleI GAGTC 8 cut(s) 230, 319, 800, 808, 950, 1007, 1036, 2087
PpsI GAGTC 8 cut(s) 230, 319, 800, 808, 950, 1007, 1036, 2087
Ppu21I YACGTR 3 cut(s) 400, 1552, 1592
PpuMI RGGWCCY 1 cut(s) 857
PshAI GACNNNNGTC 1 cut(s) 1070
Psp124BI GAGCTC 4 cut(s) 188, 231, 891, 1902
Psp1406I AACGTT 1 cut(s) 864
Psp5II RGGWCCY 1 cut(s) 857
Psp6I CCWGG 1 cut(s) 783
PspGI CCWGG 1 cut(s) 783
PspOMI GGGCCC 1 cut(s) 1705
PspPI GGNCC 7 cut(s) 857, 1091, 1179, 1705, 1706, 1828, 2022
PspPPI RGGWCCY 1 cut(s) 857
PstI CTGCAG 1 cut(s) 55
PsuI RGATCY 2 cut(s) 1579, 1954
PsyI GACNNNGTC 2 cut(s) 506, 1202
PvuI CGATCG 2 cut(s) 1545, 1767
PvuII CAGCTG 1 cut(s) 872
RgaI GCGATCGC 1 cut(s) 1767
RsaI GTAC 4 cut(s) 1304, 1320, 1550, 1913
RsaNI GTAC 4 cut(s) 1303, 1319, 1549, 1912
RseI CAYNNNNRTG 2 cut(s) 1288, 1976
SacI GAGCTC 4 cut(s) 188, 231, 891, 1902
SalI GTCGAC 4 cut(s) 581, 1030, 1147, 1373
SaqAI TTAA 5 cut(s) 116, 305, 1464, 1733, 2098
Sau3AI GATC 6 cut(s) 1542, 1579, 1612, 1625, 1764, 1954
Sau96I GGNCC 7 cut(s) 857, 1091, 1179, 1705, 1706, 1828, 2022
SchI GAGTC 8 cut(s) 230, 320, 800, 809, 950, 1007, 1037, 2087
ScrFI CCNGG 9 cut(s) 296, 356, 357, 372, 785, 1036, 1247, 1713, 1714
SduI GDGCHC 9 cut(s) 188, 231, 469, 627, 891, 1283, 1680, 1709, 1902
SfaAI GCGATCGC 1 cut(s) 1767
SfaNI GCATC 2 cut(s) 206, 1629
SfcI CTRYAG 2 cut(s) 51, 480
Sfr274I CTCGAG 1 cut(s) 1583
SgfI GCGATCGC 1 cut(s) 1767
SgrDI CGTCGACG 1 cut(s) 1373
SinI GGWCC 3 cut(s) 857, 1091, 1179
SlaI CTCGAG 1 cut(s) 1583
SmaI CCCGGG 2 cut(s) 357, 1714
SmiMI CAYNNNNRTG 2 cut(s) 1288, 1976
SmlI CTYRAG 1 cut(s) 1583
SmoI CTYRAG 1 cut(s) 1583
Sse9I AATT 3 cut(s) 1109, 1351, 1962
SseBI AGGCCT 1 cut(s) 1523
SspMI CTAG 2 cut(s) 518, 1862
SstI GAGCTC 4 cut(s) 188, 231, 891, 1902
StuI AGGCCT 1 cut(s) 1523
StyD4I CCNGG 9 cut(s) 294, 354, 355, 370, 783, 1034, 1245, 1711, 1712
StyI CCWWGG 1 cut(s) 2025
TaqII GACCGA 1 cut(s) 1196
TasI AATT 3 cut(s) 1109, 1351, 1962
TatI WGTACW 1 cut(s) 1302
TauI GCSGC 5 cut(s) 276, 713, 743, 1048, 1447
TfiI GAWTC 2 cut(s) 1184, 1573
Tru1I TTAA 5 cut(s) 116, 305, 1464, 1733, 2098
Tru9I TTAA 5 cut(s) 116, 305, 1464, 1733, 2098
TscAI CASTG 5 cut(s) 60, 246, 415, 1149, 1295
TseFI GTSAC 4 cut(s) 1204, 1404, 1504, 1684
TseI GCWGC 9 cut(s) 284, 707, 737, 848, 851, 872, 895, 1000, 1760
Tsp45I GTSAC 4 cut(s) 1204, 1404, 1504, 1684
TspDTI ATGAA 2 cut(s) 435, 1673
TspGWI ACGGA 3 cut(s) 23, 1633, 1703
TspMI CCCGGG 2 cut(s) 355, 1712
TspRI CASTG 5 cut(s) 60, 246, 415, 1149, 1295
Tth111I GACNNNGTC 2 cut(s) 506, 1202
Van91I CCANNNNNTGG 2 cut(s) 973, 1264
VpaK11BI GGWCC 3 cut(s) 857, 1091, 1179
XagI CCTNNNNNAGG 1 cut(s) 1086
XapI RAATTY 1 cut(s) 1351
XbaI TCTAGA 1 cut(s) 1861
XceI RCATGY 2 cut(s) 43, 973
XhoI CTCGAG 1 cut(s) 1583
XmaI CCCGGG 2 cut(s) 355, 1712
XmiI GTMKAC 4 cut(s) 582, 1031, 1148, 1374
XspI CTAG 2 cut(s) 518, 1862
ZraI GACGTC 2 cut(s) 1372, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.