Rroxscaffold_7G00176330
BHLH Family

basic helix-loop-helix protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010418
Physical Location & Seq
Forward (+)
15799136 .. 15804563
5428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00176330.1

Sequence Viewer

Length: 2109 bp
ATGGCTACACCGCCACCGAGTAGTAGCAGGCTCCGTGGCATGTTACAGTCTGCAGTGCAATCTGTTCAATGGACTTACAGTCTCTTTTGGCAAATCTGTCCCCAACAAGGGATGTTAATATGGGCAGATGGGTATTACAATGGGGCAATCAAGACGAGGAAGACTGTGCAACCTATGGAAGTGAGCTCTGAGGAGGCATCTCTTCAAAGGAGCCAACAACTTAGAGAGCTCTACGACTCACTGTCCGCAGGAGAAACAAACCAGCCAGCTCGCCGCCCTTGTGCTGCTTTGTCCCCGGAGGACTTAACCGAGTCGGAATGGTTCTACTTGATGTGTGTATCTTTCTCATTTCCCCCGGGAGTTGGGTTGCCGGGAAAAGCATACACAAGGAGGCAACACGTATGGCTCACTGGTGCAAATGAAGTAGATAGCAAAACCTTCTCCAGAGCTATTTTAGCCAAGAGTGCTCGAGTTCAAACTGTAGTGTGCATTCCTCTCTTAGACGGCGTCGTTGAGCTAGGCACAACAGATAGGGTTCCAGAAGACCTTGCCTTCGTCCAACACGTCAAAACTTTCTTCGTCGACCACCACCACCTTCCACCACCAAAGCCCGCCCTCTCGGAGCACTCCACCTCCAACCCCGCCACCTCCTCCGACCACCACCCCCGTTTCCACTCTCCACATCTCACCGCCATCCGCAACAACGCTGCCGCAAACAACGCCCCGCCCCTCCACGCAGCCGCCCAAGAAGACGAAGAGGACGAGGACGAGGAAGAAGACGACCAGGACGAAGAAGGAGAGTCCGACTCCGAAGCCGAAACCGGCCGTAACGATCGCCCTCTCGCTGCTGCTGCGGGACCCAACGTTCCGGCAGCAGAACCGAGCGAGCTCATGCAGCTCGAAATGTCCGAAGACATCCGCCTCGGCTCCCCCGACGACGCCTCCAACAACTTGGACTCGGACTTCCACATGCTGGCCGTCAGCCAGTCCGCCAACGCCGCCAATCAGCAGCGCCAAGCAGACTCGTATCGAGCCGAGTCGACCCGGAGGTGGCCGCCGGTCCAACCGCCAATGACAGCTGTCCAACCACCTCCCTCAGGACCGCTGGCATTGGAGGAATTGACCCACGACGACACACACTATTCGGAAACAGTGTCGACCATACTTCAAACCCAAGCAACCCGGTGGACCGATTCGTCGAATAATGACTACGTCACCTACTCCACCCAATCAGCTTTCGCCAAGTGGACGAGCAGGGCTGACCACCACCTCTTGGTTCCGGTCGAGGGCACATCCCAGTGGCTCCTAAAGTACATTTTGTTTAGTGTACCGTTCCTACATACGAAATATAGGGACGAGAATTCCCCCAAGTCTCACGACGTCGACGGCTCGAGCCGGTTGAGGAAGGGGACGTCACAAGACGAGCTAAGTGCTAACCATGTGTTAGCGGAGCGGCGTCGTAGAGAGAAGCTTAATGAGAGGTTTATTATATTGAGGTCTTTGGTGCCTTTTGTGACAAAAATGGACAAGGCCTCGATTTTGGGGGATACGATCGAGTACGTGAAGCAACTGCGTAAGAAGATTCAGGATCTCGAGGCACGTAACGTGCATTTGGAGGACGATCAACGGACGAGATCGGCGGGGGAGATGCAACGGTCGAGTAGCATGAAAGAGTTGCGAAGCGGGCTCACGGTGACGGAGCGCTCCCGTGTGGGCCCGCCCGGGTCGGACAAGAGGAAGTTGAGGATTGTGGAGGGGAGTGGCGGAGGGGCGATCGCTAAGCCTAAAGTGATAGAGGAACCGACACCACCTCCGCCACCACCGGCACCGGAACCGGCCCCTCCAACGCCCATGTTGACGGGAACGTCTCTAGAGGTGTCGATAATAGAAAGCGATGGATTGTTGGAGCTCCAATGCCCGTACAGAGAAGGGTTGTTACTTGATGTCATGCAAACCCTACGAGATCTCCGAATTGAGACCACGGTTGTGCAATCCTCGTTGACTAGTGGCACCTTCGTAGCTGAGCTAAGGGCCAAGGTGGGCAAGAAAACAACCATTACAGAAGTGAAGAGGGCAGTAAATCAAGTGATACCCCAATCTGACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH bHLH-MYC_N

Protein Analysis

702

Amino Acids

77.8

Weight (kDa)

5.41

Isoelectric Point (pI)

64.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 11 - 193 3.9e-53 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 478 - 525 7.1e-13 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 624 - 697 2e-15 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1195, 1864
AatII GACGTC 2 cut(s) 1383, 1415
AccB1I GGYRCC 3 cut(s) 1504, 1825, 2009
AccB7I CCANNNNNTGG 2 cut(s) 973, 1273
AccBSI CCGCTC 1 cut(s) 1453
AccI GTMKAC 4 cut(s) 582, 1040, 1157, 1383
AclI AACGTT 1 cut(s) 864
AclWI GGATC 1 cut(s) 1596
AcoI YGGCCR 3 cut(s) 823, 975, 1052
AcsI RAATTY 1 cut(s) 1360
AcyI GRCGYC 5 cut(s) 507, 939, 1380, 1412, 1456
AfaI GTAC 4 cut(s) 1313, 1329, 1559, 1922
AfeI AGCGCT 1 cut(s) 1703
AfiI CCNNNNNNNGG 8 cut(s) 107, 108, 362, 973, 1050, 1097, 1273, 1286
AflIII ACRYGT 2 cut(s) 397, 562
AgsI TTSAA 4 cut(s) 68, 206, 476, 1169
AhdI GACNNNNNGTC 2 cut(s) 78, 241
AhlI ACTAGT 1 cut(s) 2003
AjiI CACGTC 1 cut(s) 565
AjnI CCWGG 1 cut(s) 783
AleI CACNNNNGTG 1 cut(s) 1985
AloI GAACNNNNNNTCC 2 cut(s) 849, 881
Alw21I GWGCWC 6 cut(s) 188, 231, 469, 627, 891, 1911
Alw26I GTCTC 4 cut(s) 86, 1377, 1872, 1970
AlwI GGATC 1 cut(s) 1596
Ama87I CYCGRG 5 cut(s) 355, 468, 1390, 1592, 1721
Aor51HI AGCGCT 1 cut(s) 1703
AoxI GGCC 7 cut(s) 823, 975, 1052, 1530, 1714, 1836, 2031
ApaI GGGCCC 1 cut(s) 1718
ApeKI GCWGC 9 cut(s) 284, 707, 737, 845, 848, 851, 872, 895, 1009
ApoI RAATTY 1 cut(s) 1360
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
AsiSI GCGATCGC 1 cut(s) 1776
AspLEI GCGC 2 cut(s) 1014, 1704
AspS9I GGNCC 8 cut(s) 857, 1060, 1100, 1188, 1714, 1715, 1837, 2031
AsuC2I CCSGG 8 cut(s) 296, 356, 357, 372, 1045, 1183, 1722, 1723
AsuHPI GGTGA 3 cut(s) 679, 1207, 1705
AvaI CYCGRG 5 cut(s) 355, 468, 1390, 1592, 1721
AvaII GGWCC 4 cut(s) 857, 1060, 1100, 1188
AxyI CCTNAGG 1 cut(s) 1096
BaeGI GKGCMC 2 cut(s) 1292, 1718
BanI GGYRCC 3 cut(s) 1504, 1825, 2009
BanII GRGCYC 6 cut(s) 188, 231, 891, 1689, 1718, 1911
BarI GAAGNNNNNNTAC 2 cut(s) 1920, 1952
BbsI GAAGAC 5 cut(s) 167, 549, 756, 783, 918
Bbv12I GWGCWC 6 cut(s) 188, 231, 469, 627, 891, 1911
BbvI GCAGC 9 cut(s) 271, 694, 749, 832, 835, 838, 884, 907, 1021
BccI CCATC 3 cut(s) 122, 701, 1889
BceAI ACGGC 4 cut(s) 520, 810, 962, 1402
BcgI CGANNNNNNTGC 2 cut(s) 1412, 1446
BciT130I CCWGG 1 cut(s) 785
BciVI GTATCC 1 cut(s) 1540
BcnI CCSGG 8 cut(s) 296, 356, 357, 372, 1045, 1183, 1722, 1723
BcoDI GTCTC 4 cut(s) 86, 1377, 1872, 1970
BcuI ACTAGT 1 cut(s) 2003
BfaI CTAG 3 cut(s) 518, 1871, 2004
BfmI CTRYAG 2 cut(s) 51, 480
BfoI RGCGCY 2 cut(s) 1015, 1705
BfuI GTATCC 1 cut(s) 1540
BglII AGATCT 1 cut(s) 1963
BlpI GCTNAGC 2 cut(s) 1779, 2022
Bme1390I CCNGG 9 cut(s) 296, 356, 357, 372, 785, 1045, 1183, 1722, 1723
Bme18I GGWCC 4 cut(s) 857, 1060, 1100, 1188
BmeRI GACNNNNNGTC 2 cut(s) 78, 241
BmeT110I CYCGRG 5 cut(s) 355, 468, 1390, 1592, 1721
BmgBI CACGTC 1 cut(s) 565
BmgT120I GGNCC 8 cut(s) 857, 1060, 1100, 1188, 1714, 1715, 1837, 2031
BmrFI CCNGG 9 cut(s) 296, 356, 357, 372, 785, 1045, 1183, 1722, 1723
BmrI ACTGGG 1 cut(s) 1291
BmsI GCATC 2 cut(s) 206, 1638
BmuI ACTGGG 1 cut(s) 1291
BoxI GACNNNNGTC 1 cut(s) 1079
BpiI GAAGAC 5 cut(s) 167, 549, 756, 783, 918
BplI GAGNNNNNCTC 2 cut(s) 791, 823
BpmI CTGGAG 1 cut(s) 427
Bpu10I CCTNAGC 1 cut(s) 2027
Bpu1102I GCTNAGC 2 cut(s) 1779, 2022
BpuMI CCSGG 8 cut(s) 296, 356, 357, 372, 1045, 1183, 1722, 1723
BsaAI YACGTR 3 cut(s) 400, 1561, 1601
BsaHI GRCGYC 5 cut(s) 507, 939, 1380, 1412, 1456
BsaI GGTCTC 1 cut(s) 1970
BsaJI CCNNGG 8 cut(s) 34, 294, 354, 355, 922, 1721, 1980, 2034
BsaWI WCCGGW 2 cut(s) 1279, 1828
BsaXI ACNNNNNCTCC 8 cut(s) 617, 647, 926, 956, 1795, 1825, 1950, 1980
Bsc4I CCNNNNNNNGG 8 cut(s) 107, 108, 362, 973, 1050, 1097, 1273, 1286
Bse118I RCCGGY 5 cut(s) 821, 1057, 1395, 1822, 1834
Bse1I ACTGG 3 cut(s) 415, 985, 1297
Bse21I CCTNAGG 1 cut(s) 1096
BseBI CCWGG 1 cut(s) 785
BseDI CCNNGG 8 cut(s) 34, 294, 354, 355, 922, 1721, 1980, 2034
BseGI GGATG 4 cut(s) 117, 693, 915, 1292
BseLI CCNNNNNNNGG 8 cut(s) 107, 108, 362, 973, 1050, 1097, 1273, 1286
BseMII CTCAG 3 cut(s) 180, 1110, 2013
BseNI ACTGG 3 cut(s) 415, 985, 1297
BseRI GAGGAG 2 cut(s) 206, 640
BseSI GKGCMC 2 cut(s) 1292, 1718
BseX3I CGGCCG 1 cut(s) 823
BseXI GCAGC 9 cut(s) 271, 694, 749, 832, 835, 838, 884, 907, 1021
Bsh1285I CGRYCG 6 cut(s) 826, 835, 1284, 1554, 1658, 1776
BshFI GGCC 7 cut(s) 825, 977, 1054, 1532, 1716, 1838, 2033
BshNI GGYRCC 3 cut(s) 1504, 1825, 2009
BsiEI CGRYCG 6 cut(s) 826, 835, 1284, 1554, 1658, 1776
BsiHKAI GWGCWC 6 cut(s) 188, 231, 469, 627, 891, 1911
BsiHKCI CYCGRG 5 cut(s) 355, 468, 1390, 1592, 1721
BslFI GGGAC 5 cut(s) 84, 277, 870, 1367, 1423
BslI CCNNNNNNNGG 8 cut(s) 107, 108, 362, 973, 1050, 1097, 1273, 1286
BsmAI GTCTC 4 cut(s) 86, 1377, 1872, 1970
BsmBI CGTCTC 1 cut(s) 1872
BsmFI GGGAC 5 cut(s) 84, 277, 870, 1367, 1423
BsmI GAATGC 1 cut(s) 489
BsnI GGCC 7 cut(s) 825, 977, 1054, 1532, 1716, 1838, 2033
Bso31I GGTCTC 1 cut(s) 1970
BsoBI CYCGRG 5 cut(s) 355, 468, 1390, 1592, 1721
Bsp120I GGGCCC 1 cut(s) 1714
Bsp1286I GDGCHC 9 cut(s) 188, 231, 469, 627, 891, 1292, 1689, 1718, 1911
Bsp143I GATC 7 cut(s) 832, 1551, 1588, 1621, 1634, 1773, 1963
Bsp1720I GCTNAGC 2 cut(s) 1779, 2022
BspANI GGCC 7 cut(s) 825, 977, 1054, 1532, 1716, 1838, 2033
BspCNI CTCAG 3 cut(s) 181, 1109, 2014
BspMAI CTGCAG 1 cut(s) 55
BspPI GGATC 1 cut(s) 1596
BspT107I GGYRCC 3 cut(s) 1504, 1825, 2009
BspTNI GGTCTC 1 cut(s) 1970
BsrBI CCGCTC 1 cut(s) 1453
BsrFI RCCGGY 5 cut(s) 821, 1057, 1395, 1822, 1834
BsrI ACTGG 3 cut(s) 415, 985, 1297
BssAI RCCGGY 5 cut(s) 821, 1057, 1395, 1822, 1834
BssECI CCNNGG 8 cut(s) 34, 294, 354, 355, 922, 1721, 1980, 2034
BssMI GATC 7 cut(s) 832, 1551, 1588, 1621, 1634, 1773, 1963
BssNI GRCGYC 5 cut(s) 507, 939, 1380, 1412, 1456
BssT1I CCWWGG 1 cut(s) 2034
Bst2UI CCWGG 1 cut(s) 785
Bst6I CTCTTC 3 cut(s) 207, 750, 2063
BstACI GRCGYC 5 cut(s) 507, 939, 1380, 1412, 1456
BstBAI YACGTR 3 cut(s) 400, 1561, 1601
BstC8I GCNNGC 9 cut(s) 29, 267, 271, 612, 887, 975, 1107, 1685, 1718
BstDEI CTNAG 8 cut(s) 189, 221, 499, 1096, 1427, 1779, 2022, 2027
BstDSI CCRYGG 2 cut(s) 34, 1980
BstENI CCTNNNNNAGG 1 cut(s) 1095
BstF5I GGATG 4 cut(s) 117, 693, 915, 1292
BstH2I RGCGCY 2 cut(s) 1015, 1705
BstHHI GCGC 2 cut(s) 1014, 1704
BstKTI GATC 7 cut(s) 835, 1554, 1591, 1624, 1637, 1776, 1966
BstMAI GTCTC 4 cut(s) 86, 1377, 1872, 1970
BstMBI GATC 7 cut(s) 832, 1551, 1588, 1621, 1634, 1773, 1963
BstMCI CGRYCG 6 cut(s) 826, 835, 1284, 1554, 1658, 1776
BstMWI GCNNNNNNNGC 7 cut(s) 455, 464, 719, 851, 895, 998, 1684
BstNI CCWGG 1 cut(s) 785
BstNSI RCATGY 2 cut(s) 43, 973
BstPAI GACNNNNGTC 1 cut(s) 1079
BstSCI CCNGG 9 cut(s) 294, 354, 355, 370, 783, 1043, 1181, 1720, 1721
BstSFI CTRYAG 2 cut(s) 51, 480
BstSLI GKGCMC 2 cut(s) 1292, 1718
BstV1I GCAGC 9 cut(s) 271, 694, 749, 832, 835, 838, 884, 907, 1021
BstV2I GAAGAC 5 cut(s) 167, 549, 756, 783, 918
BstX2I RGATCY 2 cut(s) 1588, 1963
BstXI CCANNNNNNTGG 1 cut(s) 952
BstYI RGATCY 2 cut(s) 1588, 1963
BstZI CGGCCG 1 cut(s) 823
Bsu36I CCTNAGG 1 cut(s) 1096
BsuI GTATCC 1 cut(s) 1540
BsuRI GGCC 7 cut(s) 825, 977, 1054, 1532, 1716, 1838, 2033
BtgI CCRYGG 2 cut(s) 34, 1980
BtgZI GCGATG 1 cut(s) 1908
BtrI CACGTC 1 cut(s) 565
BtsCI GGATG 4 cut(s) 117, 693, 915, 1292
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 5 cut(s) 60, 239, 408, 1158, 1304
Cac8I GCNNGC 9 cut(s) 29, 267, 271, 612, 887, 975, 1107, 1685, 1718
CfoI GCGC 2 cut(s) 1014, 1704
Cfr10I RCCGGY 5 cut(s) 821, 1057, 1395, 1822, 1834
Cfr13I GGNCC 8 cut(s) 857, 1060, 1100, 1188, 1714, 1715, 1837, 2031
Cfr9I CCCGGG 2 cut(s) 355, 1721
CseI GACGC 3 cut(s) 496, 947, 1445
Csp6I GTAC 4 cut(s) 1312, 1328, 1558, 1921
CviAII CATG 7 cut(s) 40, 892, 970, 1439, 1666, 1852, 1948
CviQI GTAC 4 cut(s) 1312, 1328, 1558, 1921
DdeI CTNAG 8 cut(s) 189, 221, 499, 1096, 1427, 1779, 2022, 2027
DpnI GATC 7 cut(s) 834, 1553, 1590, 1623, 1636, 1775, 1965
DpnII GATC 7 cut(s) 832, 1551, 1588, 1621, 1634, 1773, 1963
DrdI GACNNNNNNGTC 2 cut(s) 1195, 1864
DriI GACNNNNNGTC 2 cut(s) 78, 241
DseDI GACNNNNNNGTC 2 cut(s) 1195, 1864
EaeI YGGCCR 3 cut(s) 823, 975, 1052
EagI CGGCCG 1 cut(s) 823
Eam1104I CTCTTC 3 cut(s) 207, 750, 2063
Eam1105I GACNNNNNGTC 2 cut(s) 78, 241
EarI CTCTTC 3 cut(s) 207, 750, 2063
EciI GGCGGA 4 cut(s) 908, 979, 1779, 1803
Ecl136II GAGCTC 4 cut(s) 186, 229, 889, 1909
EclXI CGGCCG 1 cut(s) 823
Eco130I CCWWGG 1 cut(s) 2034
Eco147I AGGCCT 1 cut(s) 1532
Eco24I GRGCYC 6 cut(s) 188, 231, 891, 1689, 1718, 1911
Eco31I GGTCTC 1 cut(s) 1970
Eco47I GGWCC 4 cut(s) 857, 1060, 1100, 1188
Eco47III AGCGCT 1 cut(s) 1703
Eco52I CGGCCG 1 cut(s) 823
Eco53kI GAGCTC 4 cut(s) 186, 229, 889, 1909
Eco81I CCTNAGG 1 cut(s) 1096
Eco88I CYCGRG 5 cut(s) 355, 468, 1390, 1592, 1721
EcoICRI GAGCTC 4 cut(s) 186, 229, 889, 1909
EcoNI CCTNNNNNAGG 1 cut(s) 1095
EcoO109I RGGNCCY 1 cut(s) 857
EcoRI GAATTC 1 cut(s) 1360
EcoRII CCWGG 1 cut(s) 783
EcoT14I CCWWGG 1 cut(s) 2034
EcoT38I GRGCYC 6 cut(s) 188, 231, 891, 1689, 1718, 1911
ErhI CCWWGG 1 cut(s) 2034
Esp3I CGTCTC 1 cut(s) 1872
FaeI CATG 7 cut(s) 43, 895, 973, 1442, 1669, 1855, 1951
FaqI GGGAC 5 cut(s) 84, 277, 870, 1367, 1423
FatI CATG 7 cut(s) 39, 891, 969, 1438, 1665, 1851, 1947
FauI CCCGC 7 cut(s) 619, 649, 732, 847, 1633, 1676, 1725
FblI GTMKAC 4 cut(s) 582, 1040, 1157, 1383
FokI GGATG 4 cut(s) 124, 680, 902, 1279
FriOI GRGCYC 6 cut(s) 188, 231, 891, 1689, 1718, 1911
FspBI CTAG 3 cut(s) 518, 1871, 2004
GlaI GCGC 2 cut(s) 1013, 1703
GsuI CTGGAG 1 cut(s) 427
HaeII RGCGCY 2 cut(s) 1015, 1705
HaeIII GGCC 7 cut(s) 825, 977, 1054, 1532, 1716, 1838, 2033
HgaI GACGC 3 cut(s) 496, 947, 1445
HhaI GCGC 2 cut(s) 1014, 1704
Hin1I GRCGYC 5 cut(s) 507, 939, 1380, 1412, 1456
Hin1II CATG 7 cut(s) 43, 895, 973, 1442, 1669, 1855, 1951
Hin6I GCGC 2 cut(s) 1012, 1702
HinP1I GCGC 2 cut(s) 1012, 1702
HincII GTYRAC 6 cut(s) 583, 1041, 1158, 1384, 1857, 2001
HindII GTYRAC 6 cut(s) 583, 1041, 1158, 1384, 1857, 2001
HindIII AAGCTT 1 cut(s) 1469
HphI GGTGA 3 cut(s) 679, 1207, 1705
Hpy166II GTNNAC 9 cut(s) 583, 1041, 1158, 1188, 1248, 1328, 1384, 1857, 2001
Hpy188III TCNNGA 8 cut(s) 151, 444, 539, 1098, 1376, 1586, 1592, 1871
Hpy8I GTNNAC 9 cut(s) 583, 1041, 1158, 1188, 1248, 1328, 1384, 1857, 2001
HpyAV CCTTC 7 cut(s) 448, 562, 605, 788, 1399, 1922, 2023
HpyF10VI GCNNNNNNNGC 7 cut(s) 455, 464, 719, 851, 895, 998, 1684
HpyF3I CTNAG 8 cut(s) 189, 221, 499, 1096, 1427, 1779, 2022, 2027
Hsp92I GRCGYC 5 cut(s) 507, 939, 1380, 1412, 1456
Hsp92II CATG 7 cut(s) 43, 895, 973, 1442, 1669, 1855, 1951
HspAI GCGC 2 cut(s) 1012, 1702
KflI GGGWCCC 1 cut(s) 857
Kzo9I GATC 7 cut(s) 832, 1551, 1588, 1621, 1634, 1773, 1963
Lsp1109I GCAGC 9 cut(s) 271, 694, 749, 832, 835, 838, 884, 907, 1021
LweI GCATC 2 cut(s) 206, 1638
MaeI CTAG 3 cut(s) 518, 1871, 2004
MaeIII GTNAC 8 cut(s) 42, 827, 1213, 1413, 1513, 1601, 1693, 1935
MalI GATC 7 cut(s) 834, 1553, 1590, 1623, 1636, 1775, 1965
MbiI CCGCTC 1 cut(s) 1453
MboI GATC 7 cut(s) 832, 1551, 1588, 1621, 1634, 1773, 1963
MflI RGATCY 2 cut(s) 1588, 1963
MhlI GDGCHC 9 cut(s) 188, 231, 469, 627, 891, 1292, 1689, 1718, 1911
MluCI AATT 3 cut(s) 1118, 1360, 1971
MlyI GAGTC 8 cut(s) 230, 320, 800, 809, 950, 1016, 1046, 2096
MseI TTAA 4 cut(s) 116, 305, 1473, 2107
MslI CAYNNNNRTG 2 cut(s) 1297, 1985
MspA1I CMGCKG 2 cut(s) 1079, 1105
MspR9I CCNGG 9 cut(s) 296, 356, 357, 372, 785, 1045, 1183, 1722, 1723
Mva1269I GAATGC 1 cut(s) 489
MvaI CCWGG 1 cut(s) 785
MwoI GCNNNNNNNGC 7 cut(s) 455, 464, 719, 851, 895, 998, 1684
NciI CCSGG 8 cut(s) 296, 356, 357, 372, 1045, 1183, 1722, 1723
NdeII GATC 7 cut(s) 832, 1551, 1588, 1621, 1634, 1773, 1963
NlaIII CATG 7 cut(s) 43, 895, 973, 1442, 1669, 1855, 1951
NmeAIII GCCGAG 2 cut(s) 903, 1060
NmuCI GTSAC 4 cut(s) 1213, 1413, 1513, 1693
NspI RCATGY 2 cut(s) 43, 973
OliI CACNNNNGTG 1 cut(s) 1985
PaeR7I CTCGAG 3 cut(s) 468, 1390, 1592
PceI AGGCCT 1 cut(s) 1532
PcsI WCGNNNNNNNCGW 6 cut(s) 561, 759, 786, 906, 930, 1966
PctI GAATGC 1 cut(s) 489
PfeI GAWTC 2 cut(s) 1193, 1582
PflFI GACNNNGTC 2 cut(s) 506, 1211
PflMI CCANNNNNTGG 2 cut(s) 973, 1273
Ple19I CGATCG 3 cut(s) 835, 1554, 1776
PleI GAGTC 8 cut(s) 230, 319, 800, 808, 950, 1016, 1045, 2096
PpsI GAGTC 8 cut(s) 230, 319, 800, 808, 950, 1016, 1045, 2096
Ppu21I YACGTR 3 cut(s) 400, 1561, 1601
PpuMI RGGWCCY 1 cut(s) 857
PshAI GACNNNNGTC 1 cut(s) 1079
Psp124BI GAGCTC 4 cut(s) 188, 231, 891, 1911
Psp1406I AACGTT 1 cut(s) 864
Psp5II RGGWCCY 1 cut(s) 857
Psp6I CCWGG 1 cut(s) 783
PspGI CCWGG 1 cut(s) 783
PspOMI GGGCCC 1 cut(s) 1714
PspPI GGNCC 8 cut(s) 857, 1060, 1100, 1188, 1714, 1715, 1837, 2031
PspPPI RGGWCCY 1 cut(s) 857
PspXI VCTCGAGB 2 cut(s) 468, 1390
PstI CTGCAG 1 cut(s) 55
PsuI RGATCY 2 cut(s) 1588, 1963
PsyI GACNNNGTC 2 cut(s) 506, 1211
PvuI CGATCG 3 cut(s) 835, 1554, 1776
PvuII CAGCTG 1 cut(s) 1079
RgaI GCGATCGC 1 cut(s) 1776
RsaI GTAC 4 cut(s) 1313, 1329, 1559, 1922
RsaNI GTAC 4 cut(s) 1312, 1328, 1558, 1921
RseI CAYNNNNRTG 2 cut(s) 1297, 1985
SacI GAGCTC 4 cut(s) 188, 231, 891, 1911
SalI GTCGAC 4 cut(s) 581, 1039, 1156, 1382
SaqAI TTAA 4 cut(s) 116, 305, 1473, 2107
Sau3AI GATC 7 cut(s) 832, 1551, 1588, 1621, 1634, 1773, 1963
Sau96I GGNCC 8 cut(s) 857, 1060, 1100, 1188, 1714, 1715, 1837, 2031
SchI GAGTC 8 cut(s) 230, 320, 800, 809, 950, 1016, 1046, 2096
ScrFI CCNGG 9 cut(s) 296, 356, 357, 372, 785, 1045, 1183, 1722, 1723
SduI GDGCHC 9 cut(s) 188, 231, 469, 627, 891, 1292, 1689, 1718, 1911
SfaAI GCGATCGC 1 cut(s) 1776
SfaNI GCATC 2 cut(s) 206, 1638
SfcI CTRYAG 2 cut(s) 51, 480
Sfr274I CTCGAG 3 cut(s) 468, 1390, 1592
SgfI GCGATCGC 1 cut(s) 1776
SgrDI CGTCGACG 1 cut(s) 1382
SinI GGWCC 4 cut(s) 857, 1060, 1100, 1188
SlaI CTCGAG 3 cut(s) 468, 1390, 1592
SmaI CCCGGG 2 cut(s) 357, 1723
SmiMI CAYNNNNRTG 2 cut(s) 1297, 1985
SmlI CTYRAG 3 cut(s) 468, 1390, 1592
SmoI CTYRAG 3 cut(s) 468, 1390, 1592
SpeI ACTAGT 1 cut(s) 2003
Sse9I AATT 3 cut(s) 1118, 1360, 1971
SseBI AGGCCT 1 cut(s) 1532
SspMI CTAG 3 cut(s) 518, 1871, 2004
SstI GAGCTC 4 cut(s) 188, 231, 891, 1911
StuI AGGCCT 1 cut(s) 1532
StyD4I CCNGG 9 cut(s) 294, 354, 355, 370, 783, 1043, 1181, 1720, 1721
StyI CCWWGG 1 cut(s) 2034
TaqII GACCGA 1 cut(s) 1205
TasI AATT 3 cut(s) 1118, 1360, 1971
TatI WGTACW 1 cut(s) 1311
TauI GCSGC 6 cut(s) 276, 713, 743, 1001, 1057, 1456
TfiI GAWTC 2 cut(s) 1193, 1582
Tru1I TTAA 4 cut(s) 116, 305, 1473, 2107
Tru9I TTAA 4 cut(s) 116, 305, 1473, 2107
TscAI CASTG 5 cut(s) 60, 246, 415, 1158, 1304
TseFI GTSAC 4 cut(s) 1213, 1413, 1513, 1693
TseI GCWGC 9 cut(s) 284, 707, 737, 845, 848, 851, 872, 895, 1009
Tsp45I GTSAC 4 cut(s) 1213, 1413, 1513, 1693
TspDTI ATGAA 2 cut(s) 435, 1682
TspGWI ACGGA 3 cut(s) 23, 1642, 1712
TspMI CCCGGG 2 cut(s) 355, 1721
TspRI CASTG 5 cut(s) 60, 246, 415, 1158, 1304
Tth111I GACNNNGTC 2 cut(s) 506, 1211
Van91I CCANNNNNTGG 2 cut(s) 973, 1273
VpaK11BI GGWCC 4 cut(s) 857, 1060, 1100, 1188
XagI CCTNNNNNAGG 1 cut(s) 1095
XapI RAATTY 1 cut(s) 1360
XbaI TCTAGA 1 cut(s) 1870
XceI RCATGY 2 cut(s) 43, 973
XhoI CTCGAG 3 cut(s) 468, 1390, 1592
XmaI CCCGGG 2 cut(s) 355, 1721
XmiI GTMKAC 4 cut(s) 582, 1040, 1157, 1383
XspI CTAG 3 cut(s) 518, 1871, 2004
ZraI GACGTC 2 cut(s) 1381, 1413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.