Rorug06G0218100
BHLH Family

basic helix-loop-helix protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010418
Physical Location & Seq
Forward (+)
34863519 .. 34863800
282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0218100.1

Sequence Viewer

Length: 282 bp
ATGTTCCGACTAGAGGACGAGTTCAGGACCTTGGTCGAGCGCGGCGGCGAGTCGCTCGAGCTCAGCAGCGCGTTTCAGAGCAAGTCAAATGGTAACCTGTCGTTTGACTCGAGGGCTAAAGACGATGAAGACGAGGAAGGGGTGATCGGCGACGGCAAGGAGCACCAGATCCCTACAGTGGAGCCAATCACGGACTACGACATCGTCATCGGACGCGCTGCTTTCCAGGACCATCAACGATCTTCACGAGATCGCGTAGCGGTGGTGGCGATCTACATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH bHLH-MYC_N

Protein Analysis

93

Amino Acids

10.46

Weight (kDa)

4.53

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 42, 71, 216, 255
AciI CCGC 3 cut(s) 42, 45, 260
AclWI GGATC 1 cut(s) 163
AfiI CCNNNNNNNGG 2 cut(s) 13, 178
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 1 cut(s) 61
AluI AGCT 1 cut(s) 61
Alw21I GWGCWC 2 cut(s) 63, 165
AlwI GGATC 1 cut(s) 163
Ama87I CYCGRG 2 cut(s) 56, 109
ApeKI GCWGC 2 cut(s) 66, 218
AspLEI GCGC 3 cut(s) 42, 71, 218
AspS9I GGNCC 2 cut(s) 27, 229
AsuHPI GGTGA 1 cut(s) 154
AvaI CYCGRG 2 cut(s) 56, 109
AvaII GGWCC 2 cut(s) 27, 229
BanII GRGCYC 1 cut(s) 63
BauI CACGAG 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 2 cut(s) 63, 165
BbvI GCAGC 2 cut(s) 78, 205
BccI CCATC 1 cut(s) 240
BceAI ACGGC 1 cut(s) 169
BciT130I CCWGG 1 cut(s) 227
BfaI CTAG 1 cut(s) 11
BfmI CTRYAG 1 cut(s) 174
BisI GCNGC 4 cut(s) 43, 46, 67, 219
BlpI GCTNAGC 1 cut(s) 62
BlsI GCNGC 4 cut(s) 44, 47, 68, 220
Bme1390I CCNGG 1 cut(s) 227
Bme18I GGWCC 2 cut(s) 27, 229
BmeT110I CYCGRG 2 cut(s) 56, 109
BmgT120I GGNCC 2 cut(s) 27, 229
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 1 cut(s) 227
BoxI GACNNNNGTC 1 cut(s) 32
BpiI GAAGAC 1 cut(s) 135
Bpu1102I GCTNAGC 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 30
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 178
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 30
BseLI CCNNNNNNNGG 2 cut(s) 13, 178
BseMII CTCAG 1 cut(s) 76
BseXI GCAGC 2 cut(s) 78, 205
Bsh1236I CGCG 4 cut(s) 42, 71, 216, 255
BsiHKAI GWGCWC 2 cut(s) 63, 165
BsiHKCI CYCGRG 2 cut(s) 56, 109
BslI CCNNNNNNNGG 2 cut(s) 13, 178
BsoBI CYCGRG 2 cut(s) 56, 109
Bsp1286I GDGCHC 2 cut(s) 63, 165
Bsp143I GATC 5 cut(s) 144, 168, 239, 250, 270
Bsp1720I GCTNAGC 1 cut(s) 62
BspACI CCGC 3 cut(s) 42, 45, 260
BspCNI CTCAG 1 cut(s) 75
BspFNI CGCG 4 cut(s) 42, 71, 216, 255
BspLI GGNNCC 1 cut(s) 183
BspPI GGATC 1 cut(s) 163
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 5 cut(s) 144, 168, 239, 250, 270
BssSI CACGAG 1 cut(s) 246
BssT1I CCWWGG 1 cut(s) 30
Bst2BI CACGAG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 1 cut(s) 178
BstDEI CTNAG 1 cut(s) 62
BstEII GGTNACC 1 cut(s) 92
BstFNI CGCG 4 cut(s) 42, 71, 216, 255
BstHHI GCGC 3 cut(s) 42, 71, 218
BstKTI GATC 5 cut(s) 147, 171, 242, 253, 273
BstMBI GATC 5 cut(s) 144, 168, 239, 250, 270
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstNI CCWGG 1 cut(s) 227
BstPAI GACNNNNGTC 1 cut(s) 32
BstPI GGTNACC 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 225
BstSFI CTRYAG 1 cut(s) 174
BstUI CGCG 4 cut(s) 42, 71, 216, 255
BstV1I GCAGC 2 cut(s) 78, 205
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 168
BstYI RGATCY 1 cut(s) 168
BtsIMutI CAGTG 1 cut(s) 183
CfoI GCGC 3 cut(s) 42, 71, 218
Cfr13I GGNCC 2 cut(s) 27, 229
CseI GACGC 1 cut(s) 222
CviJI RGCY 3 cut(s) 61, 116, 184
CviKI_1 RGCY 3 cut(s) 61, 116, 184
DdeI CTNAG 1 cut(s) 62
DpnI GATC 5 cut(s) 146, 170, 241, 252, 272
DpnII GATC 5 cut(s) 144, 168, 239, 250, 270
Ecl136II GAGCTC 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 63
Eco47I GGWCC 2 cut(s) 27, 229
Eco53kI GAGCTC 1 cut(s) 61
Eco88I CYCGRG 2 cut(s) 56, 109
Eco91I GGTNACC 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 61
EcoO109I RGGNCCY 1 cut(s) 27
EcoO65I GGTNACC 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 225
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 63
ErhI CCWWGG 1 cut(s) 30
Fnu4HI GCNGC 4 cut(s) 43, 46, 67, 219
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 4 cut(s) 43, 46, 67, 219
FspBI CTAG 1 cut(s) 11
GlaI GCGC 3 cut(s) 41, 70, 217
GluI GCNGC 4 cut(s) 43, 46, 67, 219
HgaI GACGC 1 cut(s) 222
HhaI GCGC 3 cut(s) 42, 71, 218
Hin6I GCGC 3 cut(s) 40, 69, 216
HinP1I GCGC 3 cut(s) 40, 69, 216
HinfI GANTC 2 cut(s) 50, 107
HphI GGTGA 1 cut(s) 154
Hpy188I TCNGA 3 cut(s) 8, 78, 212
Hpy188III TCNNGA 2 cut(s) 25, 246
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 1 cut(s) 131
HpyCH4III ACNGT 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
HpyF3I CTNAG 1 cut(s) 62
HspAI GCGC 3 cut(s) 40, 69, 216
Kzo9I GATC 5 cut(s) 144, 168, 239, 250, 270
LmnI GCTCC 2 cut(s) 160, 181
LpnPI CCDG 5 cut(s) 10, 110, 179, 212, 239
Lsp1109I GCAGC 2 cut(s) 78, 205
MaeI CTAG 1 cut(s) 11
MaeIII GTNAC 1 cut(s) 92
MalI GATC 5 cut(s) 146, 170, 241, 252, 272
MboI GATC 5 cut(s) 144, 168, 239, 250, 270
MboII GAAGA 2 cut(s) 140, 234
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 2 cut(s) 63, 165
MlyI GAGTC 2 cut(s) 59, 101
MmeI TCCRAC 1 cut(s) 31
MnlI CCTC 3 cut(s) 7, 105, 127
MseI TTAA 1 cut(s) 280
MspR9I CCNGG 1 cut(s) 227
MvaI CCWGG 1 cut(s) 227
MvnI CGCG 4 cut(s) 42, 71, 216, 255
MwoI GCNNNNNNNGC 1 cut(s) 266
NdeII GATC 5 cut(s) 144, 168, 239, 250, 270
NlaIV GGNNCC 1 cut(s) 183
PaeR7I CTCGAG 2 cut(s) 56, 109
PcsI WCGNNNNNNNCGW 3 cut(s) 107, 129, 244
PflFI GACNNNGTC 1 cut(s) 203
PfoI TCCNGGA 1 cut(s) 225
PkrI GCNGC 4 cut(s) 44, 47, 68, 220
PleI GAGTC 2 cut(s) 58, 101
PpsI GAGTC 2 cut(s) 58, 101
PpuMI RGGWCCY 1 cut(s) 27
PshAI GACNNNNGTC 1 cut(s) 32
Psp124BI GAGCTC 1 cut(s) 63
Psp5II RGGWCCY 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 225
PspEI GGTNACC 1 cut(s) 92
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 1 cut(s) 183
PspPI GGNCC 2 cut(s) 27, 229
PspPPI RGGWCCY 1 cut(s) 27
PspXI VCTCGAGB 2 cut(s) 56, 109
PsuI RGATCY 1 cut(s) 168
PsyI GACNNNGTC 1 cut(s) 203
SacI GAGCTC 1 cut(s) 63
SaqAI TTAA 1 cut(s) 280
SatI GCNGC 4 cut(s) 43, 46, 67, 219
Sau3AI GATC 5 cut(s) 144, 168, 239, 250, 270
Sau96I GGNCC 2 cut(s) 27, 229
SchI GAGTC 2 cut(s) 59, 101
ScrFI CCNGG 1 cut(s) 227
SduI GDGCHC 2 cut(s) 63, 165
SetI ASST 3 cut(s) 32, 63, 99
SfcI CTRYAG 1 cut(s) 174
Sfr274I CTCGAG 2 cut(s) 56, 109
SinI GGWCC 2 cut(s) 27, 229
SlaI CTCGAG 2 cut(s) 56, 109
SmlI CTYRAG 2 cut(s) 56, 109
SmoI CTYRAG 2 cut(s) 56, 109
SsiI CCGC 3 cut(s) 42, 45, 260
SspMI CTAG 1 cut(s) 11
SstI GAGCTC 1 cut(s) 63
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 1 cut(s) 178
TaqI TCGA 3 cut(s) 36, 57, 110
TauI GCSGC 2 cut(s) 45, 48
Tru1I TTAA 1 cut(s) 280
Tru9I TTAA 1 cut(s) 280
TscAI CASTG 1 cut(s) 183
TseI GCWGC 2 cut(s) 66, 218
TspDTI ATGAA 1 cut(s) 141
TspGWI ACGGA 1 cut(s) 206
TspRI CASTG 1 cut(s) 183
Tth111I GACNNNGTC 1 cut(s) 203
VpaK11BI GGWCC 2 cut(s) 27, 229
XhoI CTCGAG 2 cut(s) 56, 109
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.