MD03G1266300.v1.1
BHLH Family

basic helix-loop-helix protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010418
Physical Location & Seq
Reverse (-)
35139501 .. 35147109
7609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1266300.v1.1.491

Sequence Viewer

Length: 2118 bp
ATGGCTTTGCCGCCGCCAAGTAGCAGCCGCCTCCGCGGTATGTTGCAGGCCTCAGTCCAATCTGTTCAATGGACTTACAGTCTCTTCTGGCAAATCTGTCCCCAACAAGGGATCTTAGTATGGTCAGATGGGTACTACAATGGAGCCATCAAGACGAGGAAGACGGTGCAACCAATGGAAGTGAGTGCCGAGGAGGCGTCTCTCCAGCGGAGCCAGCAGCTAAGAGAGCTCTACGACTCTTTGTCTGCTGGAGAGACGAACCAGCCACCGGCCCGCCGCCCTTGTGCCTCCTTATCCCCGGAGGACTTAACCGAGTCCGAATGGTTCTACTTGATGTGTGTCTCATTCTCCTTTCCCCCCGGCGTCGGGTTGCCAGGTAAAGCATACGCAAGGAGGCAGCATGTATGGCTCACCGGTGCAAACGAGGTCGATAGCAAAACCTTTTCCAGAGCTATTTTGGCAAAGAGTGCTCGTATACAGACTGTGGTGTGCATTCCTCTTCTAGACGGCGTCGTAGAGTTTGGCACCACAGAGAGGGTTCCAGAAGACCACGCCTTCGTCCAACGCGTCAAAACCTTCTTCGTTGACCACCACCACCCTCCGCCCCCAAAACCCGCCCTCTCCGAGCACTCCACCTCCAACCCCACCACCTCATCCGACCACCCACATTTCCACTCCTCAAACCTCCTACCAGCCATGTCCACAGACCCTCCTCTCAACGCCGCCCAAGAAGACGATGAGGACGAAGAAGATGATAATCAGGAGGAGGACGACGGAGCCGAATCCGACTCCGAAGCTGAAACGGGCCCCAATGGTGGAGCCATTGTTCCCGCTGCAAATCCTCCTCAGGCTTTGGCCGCAGTTGCCGAGCCAAGCGAGCTCATGCAACTCGAGATGTCCGAAGACATCCGGCTCGGCTCCCCTGACGACGCCTCAAATAACTTGGACTCTGATTTCCACTTGTTAGCCTTAAGTCAGTCTAGGAATCCAGCCGATCAGCAGCGCCAAGCTGACTCGTATCGAGCCGAGTCAACCCGGCGGTGGCCGTCAGTACAAGAGCCACTGAGCAGCGGCCTTCAACCGCCGCACTCAGGACCCCTAGCGTTAGAGGAGTTGACAAATGATGACACACATTACTCGGAGACGGTCTCCACCATACTCCAGGGACAATCGGCTCGGTGGATGATGAATTCGTCCACCGATTATGCAGTTTGTTCGACTCAGTCAGCTTTCGCAAAGTGGTCGAGCCGAGTTGATCACCACCTCCTTATGCCGGTCGAAGGCACATCCCAATGGCTCTTGAAATATATCTTGTTCAGTGTACCGTTCCTCCACTCAAAATACCGTGACGAGAACTCGCCGAAATCTCACGAGGGTGAAGCCTCGACTCGGTTGAGGAAAGGGACCCCACAAGACGAGCTCAGCGCCAATCATGTGTTAGCGGAACGACGACGTAGAGAGAAGCTTAATGAGAGGTTTATTATACTAAGGTCGCTTGTGCCTTTTGTGACAAAAATGGACAAGGCTTCCATATTAGGGGACACAATCGAGTACGTGAAGCAACTGCGTAAGAAAATTCAGGATCTCGAGGCACGTAACGTGCCGATGGAGGATGATCAACAGTCGAGATCATCCGGGGAAATGCAAAGGTCGAGTAGTTGTAAAGAGTTGCGAAGTGGGCTCCCGGTAGTAGAGCGGACACAGGCCGGTCACGGGTCGGATAAAAGGAAGTTGAGGATTGTGGAAGGAAGCGGTGGCGTCGCCATTGCTAAGCCTAAAGCAATGGAGGACTTACCGCCTCCACTGCCGCCACCACCACCTCAGCCAGAACCGTCACCGACACCTATGGTGACGGGAACTTCTTTAGAGGTGTCAATAATCGAGAGTGACGGGTTGTTGGAGCTCCAATGCCCGTATAGAGAAGGGTTATTGCTTGATGTGATGCAAACACTTAGAGAGCTAAGAATTGAGACCATGGTTGTCCATTCCTCATTGAATAACGGGTACTTCGTAGCTGAACTAAGGGCCAAGGTGAAGGATAACGTGAATGGCAAGAAAATAAGTATTACGGAAGTGAAGAGGGTGATAAATCAAATTATACCTCAATCTGACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH bHLH-MYC_N

Protein Analysis

706

Amino Acids

78.23

Weight (kDa)

5.25

Isoelectric Point (pI)

65.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 11 - 194 2.1e-52 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 476 - 523 7.1e-13 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 622 - 700 1.3e-13 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 524
AccBSI CCGCTC 1 cut(s) 1696
AccI GTMKAC 1 cut(s) 475
AccII CGCG 2 cut(s) 36, 567
AclWI GGATC 2 cut(s) 119, 1590
AcoI YGGCCR 2 cut(s) 855, 1043
AcsI RAATTY 2 cut(s) 1189, 1575
AcyI GRCGYC 5 cut(s) 197, 363, 510, 930, 1758
AfaI GTAC 5 cut(s) 134, 1053, 1323, 1553, 2006
AflII CTTAAG 1 cut(s) 970
AflIII ACRYGT 1 cut(s) 565
AgeI ACCGGT 1 cut(s) 413
AgsI TTSAA 4 cut(s) 68, 1079, 1303, 1996
AhdI GACNNNNNGTC 2 cut(s) 78, 241
AjnI CCWGG 2 cut(s) 373, 1161
AleI CACNNNNGTG 1 cut(s) 1374
AloI GAACNNNNNNTCC 2 cut(s) 810, 842
Alw21I GWGCWC 6 cut(s) 231, 472, 630, 882, 1422, 1905
Alw26I GTCTC 7 cut(s) 86, 204, 248, 346, 1136, 1153, 1964
AlwI GGATC 2 cut(s) 119, 1590
Ama87I CYCGRG 2 cut(s) 890, 1586
AoxI GGCC 8 cut(s) 48, 270, 805, 855, 1043, 1072, 1704, 2025
ApaI GGGCCC 1 cut(s) 809
ApeKI GCWGC 6 cut(s) 24, 217, 397, 833, 1000, 1068
ApoI RAATTY 2 cut(s) 1189, 1575
ArsI GACNNNNNNTTYG 2 cut(s) 539, 571
AsiGI ACCGGT 1 cut(s) 413
AspLEI GCGC 2 cut(s) 1005, 1427
AspS9I GGNCC 6 cut(s) 271, 805, 806, 1094, 1404, 2025
AsuC2I CCSGG 5 cut(s) 299, 360, 1036, 1636, 1685
AsuHPI GGTGA 7 cut(s) 403, 1250, 1388, 1827, 1861, 2044, 2095
AvaI CYCGRG 2 cut(s) 890, 1586
AvaII GGWCC 2 cut(s) 1094, 1404
AxyI CCTNAGG 1 cut(s) 846
BaeGI GKGCMC 1 cut(s) 809
BanI GGYRCC 1 cut(s) 524
BanII GRGCYC 6 cut(s) 231, 809, 882, 1422, 1683, 1905
BauI CACGAG 1 cut(s) 1370
BbsI GAAGAC 4 cut(s) 167, 552, 738, 909
Bbv12I GWGCWC 6 cut(s) 231, 472, 630, 882, 1422, 1905
BbvCI CCTCAGC 1 cut(s) 1821
BbvI GCAGC 6 cut(s) 36, 229, 409, 820, 1012, 1080
BccI CCATC 3 cut(s) 122, 155, 1600
BceAI ACGGC 2 cut(s) 523, 1030
BcgI CGANNNNNNTGC 4 cut(s) 1197, 1224, 1231, 1258
BciT130I CCWGG 2 cut(s) 375, 1163
BclI TGATCA 2 cut(s) 1255, 1615
BcnI CCSGG 5 cut(s) 299, 360, 1036, 1636, 1685
BcoDI GTCTC 7 cut(s) 86, 204, 248, 346, 1136, 1153, 1964
BfaI CTAG 3 cut(s) 503, 981, 1100
BfoI RGCGCY 2 cut(s) 1006, 1428
BfrI CTTAAG 1 cut(s) 970
BglI GCCNNNNNGGC 1 cut(s) 194
BlpI GCTNAGC 2 cut(s) 1421, 1770
Bme1390I CCNGG 7 cut(s) 299, 360, 375, 1036, 1163, 1636, 1685
Bme18I GGWCC 2 cut(s) 1094, 1404
BmeRI GACNNNNNGTC 2 cut(s) 78, 241
BmeT110I CYCGRG 2 cut(s) 890, 1586
BmgT120I GGNCC 6 cut(s) 271, 805, 806, 1094, 1404, 2025
BmrFI CCNGG 7 cut(s) 299, 360, 375, 1036, 1163, 1636, 1685
BmsI GCATC 1 cut(s) 1932
BpiI GAAGAC 4 cut(s) 167, 552, 738, 909
BplI GAGNNNNNCTC 2 cut(s) 1133, 1165
BpmI CTGGAG 3 cut(s) 188, 270, 1145
Bpu10I CCTNAGC 1 cut(s) 1821
Bpu1102I GCTNAGC 2 cut(s) 1421, 1770
BpuMI CCSGG 5 cut(s) 299, 360, 1036, 1636, 1685
BsaAI YACGTR 2 cut(s) 1555, 1595
BsaBI GATNNNNATC 1 cut(s) 756
BsaHI GRCGYC 5 cut(s) 197, 363, 510, 930, 1758
BsaI GGTCTC 2 cut(s) 1153, 1964
BsaJI CCNNGG 8 cut(s) 34, 189, 297, 358, 1162, 1635, 1974, 2028
BsaWI WCCGGW 1 cut(s) 413
BsaXI ACNNNNNCTCC 8 cut(s) 620, 650, 694, 724, 810, 840, 1314, 1344
Bse118I RCCGGY 4 cut(s) 268, 413, 1273, 1706
Bse21I CCTNAGG 1 cut(s) 846
Bse3DI GCAATG 2 cut(s) 1764, 1788
Bse8I GATNNNNATC 1 cut(s) 756
BseBI CCWGG 2 cut(s) 375, 1163
BseDI CCNNGG 8 cut(s) 34, 189, 297, 358, 1162, 1635, 1974, 2028
BseGI GGATG 6 cut(s) 653, 906, 1188, 1286, 1618, 1631
BseJI GATNNNNATC 1 cut(s) 756
BseMI GCAATG 2 cut(s) 1764, 1788
BseMII CTCAG 7 cut(s) 66, 860, 1055, 1104, 1235, 1435, 1835
BseRI GAGGAG 6 cut(s) 206, 667, 702, 779, 834, 1124
BseSI GKGCMC 1 cut(s) 809
BseXI GCAGC 6 cut(s) 36, 229, 409, 820, 1012, 1080
Bsh1236I CGCG 2 cut(s) 36, 567
Bsh1285I CGRYCG 1 cut(s) 1278
BshFI GGCC 8 cut(s) 50, 272, 807, 857, 1045, 1074, 1706, 2027
BshNI GGYRCC 1 cut(s) 524
BshTI ACCGGT 1 cut(s) 413
BsiEI CGRYCG 1 cut(s) 1278
BsiHKAI GWGCWC 6 cut(s) 231, 472, 630, 882, 1422, 1905
BsiHKCI CYCGRG 2 cut(s) 890, 1586
BslFI GGGAC 4 cut(s) 84, 1179, 1417, 1553
BsmAI GTCTC 7 cut(s) 86, 204, 248, 346, 1136, 1153, 1964
BsmBI CGTCTC 3 cut(s) 204, 248, 1136
BsmFI GGGAC 4 cut(s) 84, 1179, 1417, 1553
BsmI GAATGC 1 cut(s) 492
BsnI GGCC 8 cut(s) 50, 272, 807, 857, 1045, 1074, 1706, 2027
Bso31I GGTCTC 2 cut(s) 1153, 1964
BsoBI CYCGRG 2 cut(s) 890, 1586
Bsp120I GGGCCC 1 cut(s) 805
Bsp1286I GDGCHC 8 cut(s) 231, 472, 630, 809, 882, 1422, 1683, 1905
Bsp143I GATC 6 cut(s) 111, 994, 1255, 1582, 1615, 1628
Bsp1720I GCTNAGC 2 cut(s) 1421, 1770
Bsp19I CCATGG 1 cut(s) 1974
BspANI GGCC 8 cut(s) 50, 272, 807, 857, 1045, 1074, 1706, 2027
BspCNI CTCAG 7 cut(s) 65, 859, 1056, 1103, 1234, 1434, 1834
BspFNI CGCG 2 cut(s) 36, 567
BspPI GGATC 2 cut(s) 119, 1590
BspT107I GGYRCC 1 cut(s) 524
BspTI CTTAAG 1 cut(s) 970
BspTNI GGTCTC 2 cut(s) 1153, 1964
BsrBI CCGCTC 1 cut(s) 1696
BsrDI GCAATG 2 cut(s) 1764, 1788
BsrFI RCCGGY 4 cut(s) 268, 413, 1273, 1706
BssAI RCCGGY 4 cut(s) 268, 413, 1273, 1706
BssECI CCNNGG 8 cut(s) 34, 189, 297, 358, 1162, 1635, 1974, 2028
BssMI GATC 6 cut(s) 111, 994, 1255, 1582, 1615, 1628
BssNAI GTATAC 1 cut(s) 476
BssNI GRCGYC 5 cut(s) 197, 363, 510, 930, 1758
BssSI CACGAG 1 cut(s) 1370
BssT1I CCWWGG 2 cut(s) 1974, 2028
Bst1107I GTATAC 1 cut(s) 476
Bst2BI CACGAG 1 cut(s) 1370
Bst2UI CCWGG 2 cut(s) 375, 1163
Bst4CI ACNGT 8 cut(s) 80, 166, 484, 1147, 1326, 1346, 1623, 1833
Bst6I CTCTTC 3 cut(s) 89, 504, 2072
BstACI GRCGYC 5 cut(s) 197, 363, 510, 930, 1758
BstAFI CTTAAG 1 cut(s) 970
BstAPI GCANNNNNTGC 1 cut(s) 467
BstBAI YACGTR 2 cut(s) 1555, 1595
BstC8I GCNNGC 4 cut(s) 48, 215, 274, 878
BstDSI CCRYGG 2 cut(s) 34, 1974
BstF5I GGATG 6 cut(s) 653, 906, 1188, 1286, 1618, 1631
BstFNI CGCG 2 cut(s) 36, 567
BstH2I RGCGCY 2 cut(s) 1006, 1428
BstHHI GCGC 2 cut(s) 1005, 1427
BstKTI GATC 6 cut(s) 114, 997, 1258, 1585, 1618, 1631
BstMAI GTCTC 7 cut(s) 86, 204, 248, 346, 1136, 1153, 1964
BstMBI GATC 6 cut(s) 111, 994, 1255, 1582, 1615, 1628
BstMCI CGRYCG 1 cut(s) 1278
BstNI CCWGG 2 cut(s) 375, 1163
BstNSI RCATGY 1 cut(s) 404
BstSCI CCNGG 7 cut(s) 297, 358, 373, 1034, 1161, 1634, 1683
BstSLI GKGCMC 1 cut(s) 809
BstUI CGCG 2 cut(s) 36, 567
BstV1I GCAGC 6 cut(s) 36, 229, 409, 820, 1012, 1080
BstV2I GAAGAC 4 cut(s) 167, 552, 738, 909
BstX2I RGATCY 2 cut(s) 111, 1582
BstYI RGATCY 2 cut(s) 111, 1582
BstZ17I GTATAC 1 cut(s) 476
Bsu36I CCTNAGG 1 cut(s) 846
BsuRI GGCC 8 cut(s) 50, 272, 807, 857, 1045, 1074, 1706, 2027
BtgI CCRYGG 2 cut(s) 34, 1974
BtsCI GGATG 6 cut(s) 653, 906, 1188, 1286, 1618, 1631
BtsI GCAGTG 1 cut(s) 1802
BtsIMutI CAGTG 3 cut(s) 1061, 1324, 1802
Cac8I GCNNGC 4 cut(s) 48, 215, 274, 878
CfoI GCGC 2 cut(s) 1005, 1427
Cfr10I RCCGGY 4 cut(s) 268, 413, 1273, 1706
Cfr13I GGNCC 6 cut(s) 271, 805, 806, 1094, 1404, 2025
Cfr42I CCGCGG 1 cut(s) 37
CseI GACGC 6 cut(s) 186, 352, 499, 556, 938, 1747
Csp6I GTAC 5 cut(s) 133, 1052, 1322, 1552, 2005
CspAI ACCGGT 1 cut(s) 413
CviAII CATG 5 cut(s) 401, 697, 883, 1433, 1975
CviQI GTAC 5 cut(s) 133, 1052, 1322, 1552, 2005
DpnI GATC 6 cut(s) 113, 996, 1257, 1584, 1617, 1630
DpnII GATC 6 cut(s) 111, 994, 1255, 1582, 1615, 1628
DriI GACNNNNNGTC 2 cut(s) 78, 241
EaeI YGGCCR 2 cut(s) 855, 1043
Eam1104I CTCTTC 3 cut(s) 89, 504, 2072
Eam1105I GACNNNNNGTC 2 cut(s) 78, 241
EarI CTCTTC 3 cut(s) 89, 504, 2072
EciI GGCGGA 1 cut(s) 591
Ecl136II GAGCTC 4 cut(s) 229, 880, 1420, 1903
Eco130I CCWWGG 2 cut(s) 1974, 2028
Eco147I AGGCCT 1 cut(s) 50
Eco24I GRGCYC 6 cut(s) 231, 809, 882, 1422, 1683, 1905
Eco31I GGTCTC 2 cut(s) 1153, 1964
Eco47I GGWCC 2 cut(s) 1094, 1404
Eco53kI GAGCTC 4 cut(s) 229, 880, 1420, 1903
Eco81I CCTNAGG 1 cut(s) 846
Eco88I CYCGRG 2 cut(s) 890, 1586
EcoICRI GAGCTC 4 cut(s) 229, 880, 1420, 1903
EcoO109I RGGNCCY 3 cut(s) 806, 1094, 1404
EcoRI GAATTC 1 cut(s) 1189
EcoRII CCWGG 2 cut(s) 373, 1161
EcoT14I CCWWGG 2 cut(s) 1974, 2028
EcoT38I GRGCYC 6 cut(s) 231, 809, 882, 1422, 1683, 1905
ErhI CCWWGG 2 cut(s) 1974, 2028
Esp3I CGTCTC 3 cut(s) 204, 248, 1136
FaeI CATG 5 cut(s) 404, 700, 886, 1436, 1978
FaqI GGGAC 4 cut(s) 84, 1179, 1417, 1553
FatI CATG 5 cut(s) 400, 696, 882, 1432, 1974
FauI CCCGC 3 cut(s) 281, 622, 838
FbaI TGATCA 2 cut(s) 1255, 1615
FblI GTMKAC 1 cut(s) 475
FokI GGATG 6 cut(s) 640, 893, 1195, 1273, 1618, 1625
FriOI GRGCYC 6 cut(s) 231, 809, 882, 1422, 1683, 1905
FspBI CTAG 3 cut(s) 503, 981, 1100
GlaI GCGC 2 cut(s) 1004, 1426
GsuI CTGGAG 3 cut(s) 188, 270, 1145
HaeII RGCGCY 2 cut(s) 1006, 1428
HaeIII GGCC 8 cut(s) 50, 272, 807, 857, 1045, 1074, 1706, 2027
HgaI GACGC 6 cut(s) 186, 352, 499, 556, 938, 1747
HhaI GCGC 2 cut(s) 1005, 1427
Hin1I GRCGYC 5 cut(s) 197, 363, 510, 930, 1758
Hin1II CATG 5 cut(s) 404, 700, 886, 1436, 1978
Hin6I GCGC 2 cut(s) 1003, 1425
HinP1I GCGC 2 cut(s) 1003, 1425
HincII GTYRAC 3 cut(s) 586, 1032, 1116
HindII GTYRAC 3 cut(s) 586, 1032, 1116
HindIII AAGCTT 1 cut(s) 1463
HphI GGTGA 7 cut(s) 403, 1250, 1388, 1827, 1861, 2044, 2095
Hpy166II GTNNAC 7 cut(s) 476, 586, 702, 1032, 1116, 1197, 1322
Hpy8I GTNNAC 7 cut(s) 476, 586, 702, 1032, 1116, 1197, 1322
Hpy99I CGWCG 7 cut(s) 368, 515, 776, 932, 1452, 1455, 1763
HpyAV CCTTC 7 cut(s) 565, 586, 1085, 1274, 1739, 1916, 2029
HpyCH4III ACNGT 8 cut(s) 80, 166, 484, 1147, 1326, 1346, 1623, 1833
HpyCH4IV ACGT 5 cut(s) 1453, 1554, 1594, 1599, 2043
HpyCH4V TGCA 9 cut(s) 46, 169, 419, 492, 836, 886, 1208, 1645, 1945
HpySE526I ACGT 5 cut(s) 1453, 1554, 1594, 1599, 2043
Hsp92I GRCGYC 5 cut(s) 197, 363, 510, 930, 1758
Hsp92II CATG 5 cut(s) 404, 700, 886, 1436, 1978
HspAI GCGC 2 cut(s) 1003, 1425
KflI GGGWCCC 1 cut(s) 1404
Ksp22I TGATCA 2 cut(s) 1255, 1615
KspI CCGCGG 1 cut(s) 37
Kzo9I GATC 6 cut(s) 111, 994, 1255, 1582, 1615, 1628
LmnI GCTCC 8 cut(s) 143, 210, 776, 818, 923, 1686, 1900, 1908
Lsp1109I GCAGC 6 cut(s) 36, 229, 409, 820, 1012, 1080
LweI GCATC 1 cut(s) 1932
MaeI CTAG 3 cut(s) 503, 981, 1100
MaeII ACGT 5 cut(s) 1453, 1554, 1594, 1599, 2043
MaeIII GTNAC 7 cut(s) 1346, 1507, 1595, 1709, 1833, 1849, 1886
MalI GATC 6 cut(s) 113, 996, 1257, 1584, 1617, 1630
MbiI CCGCTC 1 cut(s) 1696
MboI GATC 6 cut(s) 111, 994, 1255, 1582, 1615, 1628
MflI RGATCY 2 cut(s) 111, 1582
MhlI GDGCHC 8 cut(s) 231, 472, 630, 809, 882, 1422, 1683, 1905
MluCI AATT 4 cut(s) 1189, 1575, 1965, 2094
MluI ACGCGT 1 cut(s) 565
MlyI GAGTC 9 cut(s) 230, 323, 782, 941, 1007, 1037, 1213, 1381, 2105
MmeI TCCRAC 6 cut(s) 586, 663, 681, 810, 1698, 1878
MseI TTAA 4 cut(s) 308, 971, 1467, 2116
MslI CAYNNNNRTG 2 cut(s) 1291, 1374
MspA1I CMGCKG 4 cut(s) 36, 208, 833, 1071
MspCI CTTAAG 1 cut(s) 970
MspR9I CCNGG 7 cut(s) 299, 360, 375, 1036, 1163, 1636, 1685
Mva1269I GAATGC 1 cut(s) 492
MvaI CCWGG 2 cut(s) 375, 1163
MvnI CGCG 2 cut(s) 36, 567
NciI CCSGG 5 cut(s) 299, 360, 1036, 1636, 1685
NcoI CCATGG 1 cut(s) 1974
NdeII GATC 6 cut(s) 111, 994, 1255, 1582, 1615, 1628
NlaIII CATG 5 cut(s) 404, 700, 886, 1436, 1978
NmeAIII GCCGAG 5 cut(s) 214, 892, 894, 1051, 1274
NmuCI GTSAC 6 cut(s) 1346, 1507, 1709, 1833, 1849, 1886
NspI RCATGY 1 cut(s) 404
OliI CACNNNNGTG 1 cut(s) 1374
PaeR7I CTCGAG 2 cut(s) 890, 1586
PceI AGGCCT 1 cut(s) 50
PcsI WCGNNNNNNNCGW 5 cut(s) 564, 741, 777, 897, 2007
PctI GAATGC 1 cut(s) 492
PfeI GAWTC 2 cut(s) 782, 985
PflFI GACNNNGTC 2 cut(s) 509, 1222
PinAI ACCGGT 1 cut(s) 413
PleI GAGTC 9 cut(s) 230, 322, 782, 941, 1007, 1036, 1213, 1381, 2105
PpsI GAGTC 9 cut(s) 230, 322, 782, 941, 1007, 1036, 1213, 1381, 2105
Ppu21I YACGTR 2 cut(s) 1555, 1595
PpuMI RGGWCCY 2 cut(s) 1094, 1404
Psp124BI GAGCTC 4 cut(s) 231, 882, 1422, 1905
Psp5II RGGWCCY 2 cut(s) 1094, 1404
Psp6I CCWGG 2 cut(s) 373, 1161
PspGI CCWGG 2 cut(s) 373, 1161
PspOMI GGGCCC 1 cut(s) 805
PspPI GGNCC 6 cut(s) 271, 805, 806, 1094, 1404, 2025
PspPPI RGGWCCY 2 cut(s) 1094, 1404
PsuI RGATCY 2 cut(s) 111, 1582
PsyI GACNNNGTC 2 cut(s) 509, 1222
RsaI GTAC 5 cut(s) 134, 1053, 1323, 1553, 2006
RsaNI GTAC 5 cut(s) 133, 1052, 1322, 1552, 2005
RseI CAYNNNNRTG 2 cut(s) 1291, 1374
SacI GAGCTC 4 cut(s) 231, 882, 1422, 1905
SacII CCGCGG 1 cut(s) 37
SaqAI TTAA 4 cut(s) 308, 971, 1467, 2116
Sau3AI GATC 6 cut(s) 111, 994, 1255, 1582, 1615, 1628
Sau96I GGNCC 6 cut(s) 271, 805, 806, 1094, 1404, 2025
SchI GAGTC 9 cut(s) 230, 323, 782, 941, 1007, 1037, 1213, 1381, 2105
ScrFI CCNGG 7 cut(s) 299, 360, 375, 1036, 1163, 1636, 1685
SduI GDGCHC 8 cut(s) 231, 472, 630, 809, 882, 1422, 1683, 1905
SfaNI GCATC 1 cut(s) 1932
Sfr274I CTCGAG 2 cut(s) 890, 1586
Sfr303I CCGCGG 1 cut(s) 37
SgrAI CRCCGGYG 1 cut(s) 413
SgrBI CCGCGG 1 cut(s) 37
SinI GGWCC 2 cut(s) 1094, 1404
SlaI CTCGAG 2 cut(s) 890, 1586
SmiMI CAYNNNNRTG 2 cut(s) 1291, 1374
SmlI CTYRAG 3 cut(s) 890, 970, 1586
SmoI CTYRAG 3 cut(s) 890, 970, 1586
Sse9I AATT 4 cut(s) 1189, 1575, 1965, 2094
SseBI AGGCCT 1 cut(s) 50
SspMI CTAG 3 cut(s) 503, 981, 1100
SstI GAGCTC 4 cut(s) 231, 882, 1422, 1905
StuI AGGCCT 1 cut(s) 50
StyD4I CCNGG 7 cut(s) 297, 358, 373, 1034, 1161, 1634, 1683
StyI CCWWGG 2 cut(s) 1974, 2028
TaaI ACNGT 8 cut(s) 80, 166, 484, 1147, 1326, 1346, 1623, 1833
TaiI ACGT 5 cut(s) 1456, 1557, 1597, 1602, 2046
TasI AATT 4 cut(s) 1189, 1575, 1965, 2094
TatI WGTACW 1 cut(s) 1051
TauI GCSGC 9 cut(s) 13, 16, 30, 279, 725, 860, 1074, 1087, 1810
TfiI GAWTC 2 cut(s) 782, 985
Tru1I TTAA 4 cut(s) 308, 971, 1467, 2116
Tru9I TTAA 4 cut(s) 308, 971, 1467, 2116
TscAI CASTG 3 cut(s) 1068, 1324, 1809
TseFI GTSAC 6 cut(s) 1346, 1507, 1709, 1833, 1849, 1886
TseI GCWGC 6 cut(s) 24, 217, 397, 833, 1000, 1068
Tsp45I GTSAC 6 cut(s) 1346, 1507, 1709, 1833, 1849, 1886
TspDTI ATGAA 1 cut(s) 1202
TspGWI ACGGA 2 cut(s) 789, 2084
TspRI CASTG 3 cut(s) 1068, 1324, 1809
Tth111I GACNNNGTC 2 cut(s) 509, 1222
Vha464I CTTAAG 1 cut(s) 970
VpaK11BI GGWCC 2 cut(s) 1094, 1404
XapI RAATTY 2 cut(s) 1189, 1575
XbaI TCTAGA 1 cut(s) 502
XceI RCATGY 1 cut(s) 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 454
XhoI CTCGAG 2 cut(s) 890, 1586
XmiI GTMKAC 1 cut(s) 475
XspI CTAG 3 cut(s) 503, 981, 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.