Rorug06G0218000
BHLH Family

basic helix-loop-helix protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010418
Physical Location & Seq
Forward (+)
34851430 .. 34853564
2135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0218000.1

Sequence Viewer

Length: 411 bp
ATGATGCTCAGGTATCTTGAACAAACAACTAATTTTTTTGGTTCCTGGGCACCAGTAATCCAATTGCTCAAATCAGATGTGTTCTTAAAGAAGACATTGAAGTTAAGCAACGAGGACATTATTGGCTCTGGAGAGTATGGGACAATTTATATACTTACACAGGATGACACCATGGCCTTTGCTGTGAAAAGATTGAACAGAGGAAGTGCAGATAGAGATCGAAGATTTGAGAGGGAAATTGAAGCAATAGGGGATATAAAGCACCGGAACATCGTGACTCTTAATGGATATTACACTGCTCCACATTATAACCTTCTTATATACGAACTGGTAGCTTATGAAGGCTTGGATGGAGTGCTTCATGATCACTATCCAACAAGCATTTGGATTGGCTGTCTAGATACAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH bHLH-MYC_N

Protein Analysis

136

Amino Acids

15.7

Weight (kDa)

5.76

Isoelectric Point (pI)

28.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 37 - 123 2.7e-11 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 38 - 123 7.9e-10 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 309
AccB1I GGYRCC 1 cut(s) 49
AgsI TTSAA 4 cut(s) 20, 100, 196, 242
AjnI CCWGG 1 cut(s) 44
AluBI AGCT 1 cut(s) 335
AluI AGCT 1 cut(s) 335
AoxI GGCC 1 cut(s) 174
BaeGI GKGCMC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 49
BbsI GAAGAC 1 cut(s) 98
BccI CCATC 1 cut(s) 344
BciT130I CCWGG 1 cut(s) 46
BclI TGATCA 1 cut(s) 364
BfaI CTAG 1 cut(s) 398
Bme1390I CCNGG 1 cut(s) 46
BmiI GGNNCC 2 cut(s) 43, 51
BmrFI CCNGG 1 cut(s) 46
BpiI GAAGAC 1 cut(s) 98
BpmI CTGGAG 1 cut(s) 150
Bpu10I CCTNAGC 1 cut(s) 8
BsaBI GATNNNNATC 2 cut(s) 216, 369
BsaJI CCNNGG 2 cut(s) 45, 171
BsaWI WCCGGW 1 cut(s) 264
Bse1I ACTGG 2 cut(s) 53, 333
Bse8I GATNNNNATC 2 cut(s) 216, 369
BseBI CCWGG 1 cut(s) 46
BseDI CCNNGG 2 cut(s) 45, 171
BseGI GGATG 2 cut(s) 169, 355
BseJI GATNNNNATC 2 cut(s) 216, 369
BseMII CTCAG 1 cut(s) 22
BseNI ACTGG 2 cut(s) 53, 333
BseSI GKGCMC 1 cut(s) 52
BsgI GTGCAG 1 cut(s) 228
BshFI GGCC 1 cut(s) 176
BshNI GGYRCC 1 cut(s) 49
BsiSI CCGG 1 cut(s) 265
BslFI GGGAC 1 cut(s) 154
BsmFI GGGAC 1 cut(s) 154
BsnI GGCC 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 52
Bsp143I GATC 2 cut(s) 217, 364
Bsp19I CCATGG 1 cut(s) 171
BspANI GGCC 1 cut(s) 176
BspCNI CTCAG 1 cut(s) 21
BspHI TCATGA 1 cut(s) 361
BspLI GGNNCC 2 cut(s) 43, 51
BspT107I GGYRCC 1 cut(s) 49
BsrI ACTGG 2 cut(s) 53, 333
BssECI CCNNGG 2 cut(s) 45, 171
BssMI GATC 2 cut(s) 217, 364
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 46
BstDEI CTNAG 1 cut(s) 8
BstDSI CCRYGG 1 cut(s) 171
BstF5I GGATG 2 cut(s) 169, 355
BstKTI GATC 2 cut(s) 220, 367
BstMBI GATC 2 cut(s) 217, 364
BstNI CCWGG 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 44
BstSLI GKGCMC 1 cut(s) 52
BstV2I GAAGAC 1 cut(s) 98
BsuRI GGCC 1 cut(s) 176
BtgI CCRYGG 1 cut(s) 171
BtsCI GGATG 2 cut(s) 169, 355
BtsI GCAGTG 1 cut(s) 294
BtsIMutI CAGTG 1 cut(s) 294
CciI TCATGA 1 cut(s) 361
CviAII CATG 2 cut(s) 172, 362
CviJI RGCY 5 cut(s) 126, 176, 335, 345, 393
CviKI_1 RGCY 5 cut(s) 126, 176, 335, 345, 393
DdeI CTNAG 1 cut(s) 8
DpnI GATC 2 cut(s) 219, 366
DpnII GATC 2 cut(s) 217, 364
Eco130I CCWWGG 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 44
EcoT14I CCWWGG 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 2 cut(s) 175, 365
FaqI GGGAC 1 cut(s) 154
FatI CATG 2 cut(s) 171, 361
FbaI TGATCA 1 cut(s) 364
FokI GGATG 2 cut(s) 176, 362
FspBI CTAG 1 cut(s) 398
GsuI CTGGAG 1 cut(s) 150
HaeIII GGCC 1 cut(s) 176
HapII CCGG 1 cut(s) 265
Hin1II CATG 2 cut(s) 175, 365
HinfI GANTC 1 cut(s) 277
HpaII CCGG 1 cut(s) 265
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 5 cut(s) 17, 129, 274, 362, 398
HpyAV CCTTC 2 cut(s) 323, 335
HpyCH4V TGCA 1 cut(s) 209
HpyF3I CTNAG 1 cut(s) 8
Hsp92II CATG 2 cut(s) 175, 365
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 2 cut(s) 217, 364
LmnI GCTCC 1 cut(s) 304
LpnPI CCDG 7 cut(s) 31, 58, 66, 114, 146, 278, 314
MaeI CTAG 1 cut(s) 398
MaeIII GTNAC 1 cut(s) 274
MalI GATC 2 cut(s) 219, 366
MboI GATC 2 cut(s) 217, 364
MboII GAAGA 2 cut(s) 103, 234
MfeI CAATTG 1 cut(s) 62
MhlI GDGCHC 1 cut(s) 52
MluCI AATT 4 cut(s) 31, 62, 144, 237
MlyI GAGTC 1 cut(s) 271
MmeI TCCRAC 1 cut(s) 398
MnlI CCTC 3 cut(s) 106, 194, 225
MseI TTAA 3 cut(s) 86, 104, 282
MspI CCGG 1 cut(s) 265
MspR9I CCNGG 1 cut(s) 46
MunI CAATTG 1 cut(s) 62
MvaI CCWGG 1 cut(s) 46
NcoI CCATGG 1 cut(s) 171
NdeII GATC 2 cut(s) 217, 364
NlaIII CATG 2 cut(s) 175, 365
NlaIV GGNNCC 2 cut(s) 43, 51
NmuCI GTSAC 1 cut(s) 274
PagI TCATGA 1 cut(s) 361
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
PsiI TTATAA 1 cut(s) 309
Psp6I CCWGG 1 cut(s) 44
PspGI CCWGG 1 cut(s) 44
PspN4I GGNNCC 2 cut(s) 43, 51
SaqAI TTAA 3 cut(s) 86, 104, 282
Sau3AI GATC 2 cut(s) 217, 364
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 1 cut(s) 46
SduI GDGCHC 1 cut(s) 52
SetI ASST 3 cut(s) 14, 315, 337
Sse9I AATT 4 cut(s) 31, 62, 144, 237
SspMI CTAG 1 cut(s) 398
StyD4I CCNGG 1 cut(s) 44
StyI CCWWGG 1 cut(s) 171
TaqI TCGA 1 cut(s) 220
TasI AATT 4 cut(s) 31, 62, 144, 237
Tru1I TTAA 3 cut(s) 86, 104, 282
Tru9I TTAA 3 cut(s) 86, 104, 282
TscAI CASTG 1 cut(s) 301
TseFI GTSAC 1 cut(s) 274
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 2 cut(s) 350, 354
TspRI CASTG 1 cut(s) 301
XbaI TCTAGA 1 cut(s) 397
XcmI CCANNNNNNNNNTGG 1 cut(s) 381
XspI CTAG 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.