FvH4_2g23830

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017506
Physical Location & Seq
Reverse (-)
19470316 .. 19474068
3753 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g23830.t1

Sequence Viewer

Length: 1071 bp
ATGGCTATGGAGAAGCTGCAAATGGCAGAGGGAAGGCCTATGCCTGCAGTCTTGGGTCTTTTAGTGGGGTACGACCTTATGGATTGGTCGTATCAAATTGCACTAGGAGGGGAGTGTGGAAAAATAAAAACGGCGGGTGACGGTGACCGGCCTCAGCTACTAAATCTGGCAAACTTGAGAGCCCGCTCGTCGTTTAGGCCTGATGGGGCCCCCTCTCTCCACGGCGACGACGGCCGTCATCTCAGGTCCTGGCGTCGCAGACTCCTCTCCTCCCTCTCCTCCGCCTCCGCCCTCAGATTCGCCCTCCTCCTCTTGCTCCTCTTCCTCCTCGCCACCGCCTTCATTTCCCTCCCTATCGAAAAGAGTTTGAAGGATTTCTTGCTATGGATCAAGAAGGATCTTGGACCTTGGGGTCCGCTTGTACTGGCTGTTGCATACATTCCTCTCACGGTCCTGGCAGTTCCGGCTTCTGTACTTACACTTGGTGGAGGTTATCTTTTTGGTTTGCCTGTGGGATTCGTTGCAGACTCTCTCGGTGCAACATTAGGTGCAACAGCGGCATTCTTACTTGGGAGAACAATGGGAAGATCTTATGTTATTTCCAAACTGAAGCATTATCCTAAGTTTCAAGCTGTTGCTGTTGCCATTCAGAGATCCGGTTTCAAGATAGTTTTGCTGCTGCGGCTTGCTCCTTTGCTTCCATTTAACATGCTGAACTACCTACTCTCTGTGACTCCTGTTCACATAGGGGACTTTATGCTGGCAACTTGGTTGGGAATGATGCCCATCACATTTGCACTGGTATATGTTGGTACAACTCTTAAGGATCTTTCAGATGTTACACATGGATGGAATGAAGTTTCAACAACTCGTTGGGTGTTTATAGTACTTGGTTTTGGAATATCCGTGGTTCTTCTGATTTGCATTAGTAAAGTAGCAAAGGCTTCTTTGGAGAAAGCACTGGCTGACAATGCTGAAATAGAAGGGATATTCTCATCCTCGACGCTGCCCATTGTGGCAGATTCATCCCCGGATCTTCCAAAAACTCTCATCATACAGATAGACTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

357

Amino Acids

38.49

Weight (kDa)

9.37

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 154 - 272 1.9e-24 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03260
fragaria_vesca FvH4_2g23830
malus_domestica MD03G1267800.v1.1
rosa_chinensis RchiOBHm_Chr6g0291411
rosa_laevigata RLG00000012116
rosa_multiflora Rmu_sc0003735.1_g000021
rosa_roxburghii Rroxscaffold_7G00176070
rosa_rugosa Rorug06G0219100
rosa_samantha Rh6AG331400 Rh6BG338300 Rh6CG344600
rosa_wichuraiana Rw6G028770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 411
AccBSI CCGCTC 1 cut(s) 186
AciI CCGC 8 cut(s) 134, 184, 282, 288, 336, 416, 557, 682
AclWI GGATC 5 cut(s) 395, 405, 648, 834, 1041
AcoI YGGCCR 1 cut(s) 232
AcuI CTGAAG 1 cut(s) 629
AcyI GRCGYC 1 cut(s) 253
AdeI CACNNNGTG 1 cut(s) 485
AfaI GTAC 5 cut(s) 71, 423, 474, 814, 888
AflII CTTAAG 1 cut(s) 821
AgsI TTSAA 4 cut(s) 370, 629, 664, 864
AjnI CCWGG 2 cut(s) 248, 453
AluBI AGCT 3 cut(s) 16, 157, 632
AluI AGCT 3 cut(s) 16, 157, 632
AlwI GGATC 5 cut(s) 395, 405, 648, 834, 1041
AlwNI CAGNNNCTG 1 cut(s) 249
AoxI GGCC 5 cut(s) 35, 149, 197, 207, 232
ApaI GGGCCC 1 cut(s) 211
ApeKI GCWGC 4 cut(s) 16, 676, 679, 1006
Asp700I GAANNNNTTC 1 cut(s) 374
AspS9I GGNCC 6 cut(s) 207, 208, 246, 404, 413, 451
AsuC2I CCSGG 1 cut(s) 1031
AsuHPI GGTGA 2 cut(s) 149, 155
AvaII GGWCC 4 cut(s) 246, 404, 413, 451
BaeGI GKGCMC 1 cut(s) 211
BanII GRGCYC 2 cut(s) 184, 211
BbvCI CCTCAGC 1 cut(s) 153
BbvI GCAGC 4 cut(s) 3, 663, 666, 993
BccI CCATC 3 cut(s) 197, 794, 843
BceAI ACGGC 4 cut(s) 147, 219, 238, 247
BciT130I CCWGG 2 cut(s) 250, 455
BcnI CCSGG 1 cut(s) 1031
BfaI CTAG 1 cut(s) 104
BfmI CTRYAG 1 cut(s) 45
BfrI CTTAAG 1 cut(s) 821
BglII AGATCT 1 cut(s) 587
BisI GCNGC 6 cut(s) 17, 558, 677, 680, 683, 1007
BlsI GCNGC 6 cut(s) 18, 559, 678, 681, 684, 1008
BmcAI AGTACT 1 cut(s) 888
Bme1390I CCNGG 3 cut(s) 250, 455, 1031
Bme18I GGWCC 4 cut(s) 246, 404, 413, 451
BmgT120I GGNCC 6 cut(s) 207, 208, 246, 404, 413, 451
BmiI GGNNCC 4 cut(s) 208, 209, 210, 414
BmrFI CCNGG 3 cut(s) 250, 455, 1031
BmsI GCATC 1 cut(s) 771
BoxI GACNNNNGTC 1 cut(s) 234
Bpu10I CCTNAGC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 196
BpuMI CCSGG 1 cut(s) 1031
BsaBI GATNNNNATC 1 cut(s) 785
BsaHI GRCGYC 1 cut(s) 253
BsaJI CCNNGG 4 cut(s) 220, 407, 906, 1029
BsaWI WCCGGW 1 cut(s) 656
Bse118I RCCGGY 1 cut(s) 147
Bse1I ACTGG 3 cut(s) 429, 804, 966
Bse8I GATNNNNATC 1 cut(s) 785
BseBI CCWGG 2 cut(s) 250, 455
BseDI CCNNGG 4 cut(s) 220, 407, 906, 1029
BseGI GGATG 3 cut(s) 854, 995, 1025
BseJI GATNNNNATC 1 cut(s) 785
BseMII CTCAG 3 cut(s) 167, 256, 307
BseNI ACTGG 3 cut(s) 429, 804, 966
BseRI GAGGAG 7 cut(s) 254, 259, 268, 296, 299, 308, 317
BseSI GKGCMC 1 cut(s) 211
BseX3I CGGCCG 1 cut(s) 232
BseXI GCAGC 4 cut(s) 3, 663, 666, 993
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 5 cut(s) 37, 151, 199, 209, 234
BsiEI CGRYCG 1 cut(s) 235
BsiSI CCGG 4 cut(s) 148, 464, 657, 1031
BslFI GGGAC 1 cut(s) 764
BsmFI GGGAC 1 cut(s) 764
BsmI GAATGC 1 cut(s) 560
BsnI GGCC 5 cut(s) 37, 151, 199, 209, 234
Bsp120I GGGCCC 1 cut(s) 207
Bsp1286I GDGCHC 2 cut(s) 184, 211
Bsp143I GATC 6 cut(s) 387, 397, 587, 653, 826, 1033
BspACI CCGC 8 cut(s) 134, 184, 282, 288, 336, 416, 557, 682
BspANI GGCC 5 cut(s) 37, 151, 199, 209, 234
BspCNI CTCAG 3 cut(s) 166, 255, 306
BspLI GGNNCC 4 cut(s) 208, 209, 210, 414
BspMAI CTGCAG 1 cut(s) 49
BspPI GGATC 5 cut(s) 395, 405, 648, 834, 1041
BspTI CTTAAG 1 cut(s) 821
BsrBI CCGCTC 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 147
BsrI ACTGG 3 cut(s) 429, 804, 966
BssAI RCCGGY 1 cut(s) 147
BssECI CCNNGG 4 cut(s) 220, 407, 906, 1029
BssMI GATC 6 cut(s) 387, 397, 587, 653, 826, 1033
BssNI GRCGYC 1 cut(s) 253
BssT1I CCWWGG 1 cut(s) 407
Bst2UI CCWGG 2 cut(s) 250, 455
Bst4CI ACNGT 2 cut(s) 143, 451
Bst6I CTCTTC 1 cut(s) 326
BstACI GRCGYC 1 cut(s) 253
BstAFI CTTAAG 1 cut(s) 821
BstC8I GCNNGC 4 cut(s) 45, 184, 687, 762
BstDEI CTNAG 4 cut(s) 153, 242, 293, 621
BstDSI CCRYGG 2 cut(s) 220, 906
BstEII GGTNACC 1 cut(s) 143
BstF5I GGATG 3 cut(s) 854, 995, 1025
BstKTI GATC 6 cut(s) 390, 400, 590, 656, 829, 1036
BstMBI GATC 6 cut(s) 387, 397, 587, 653, 826, 1033
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 5 cut(s) 231, 464, 557, 682, 971
BstNI CCWGG 2 cut(s) 250, 455
BstNSI RCATGY 1 cut(s) 712
BstPAI GACNNNNGTC 1 cut(s) 234
BstPI GGTNACC 1 cut(s) 143
BstSCI CCNGG 3 cut(s) 248, 453, 1029
BstSFI CTRYAG 1 cut(s) 45
BstSLI GKGCMC 1 cut(s) 211
BstV1I GCAGC 4 cut(s) 3, 663, 666, 993
BstX2I RGATCY 5 cut(s) 397, 587, 653, 826, 1033
BstYI RGATCY 5 cut(s) 397, 587, 653, 826, 1033
BstZI CGGCCG 1 cut(s) 232
BsuRI GGCC 5 cut(s) 37, 151, 199, 209, 234
BtgI CCRYGG 2 cut(s) 220, 906
BtsCI GGATG 3 cut(s) 854, 995, 1025
BtsIMutI CAGTG 2 cut(s) 797, 959
Cac8I GCNNGC 4 cut(s) 45, 184, 687, 762
CaiI CAGNNNCTG 1 cut(s) 249
Cfr10I RCCGGY 1 cut(s) 147
Cfr13I GGNCC 6 cut(s) 207, 208, 246, 404, 413, 451
CseI GACGC 2 cut(s) 242, 1012
Csp6I GTAC 5 cut(s) 70, 422, 473, 813, 887
CviAII CATG 2 cut(s) 709, 845
CviQI GTAC 5 cut(s) 70, 422, 473, 813, 887
DdeI CTNAG 4 cut(s) 153, 242, 293, 621
DpnI GATC 6 cut(s) 389, 399, 589, 655, 828, 1035
DpnII GATC 6 cut(s) 387, 397, 587, 653, 826, 1033
DraIII CACNNNGTG 1 cut(s) 485
DrdI GACNNNNNNGTC 1 cut(s) 411
DseDI GACNNNNNNGTC 1 cut(s) 411
EaeI YGGCCR 1 cut(s) 232
EagI CGGCCG 1 cut(s) 232
Eam1104I CTCTTC 1 cut(s) 326
EarI CTCTTC 1 cut(s) 326
EciI GGCGGA 2 cut(s) 271, 277
EclXI CGGCCG 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 407
Eco147I AGGCCT 2 cut(s) 37, 199
Eco24I GRGCYC 2 cut(s) 184, 211
Eco47I GGWCC 4 cut(s) 246, 404, 413, 451
Eco52I CGGCCG 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 629
Eco91I GGTNACC 1 cut(s) 143
EcoO109I RGGNCCY 3 cut(s) 207, 208, 246
EcoO65I GGTNACC 1 cut(s) 143
EcoRII CCWGG 2 cut(s) 248, 453
EcoT14I CCWWGG 1 cut(s) 407
EcoT38I GRGCYC 2 cut(s) 184, 211
ErhI CCWWGG 1 cut(s) 407
FaeI CATG 2 cut(s) 712, 848
FalI AAGNNNNNCTT 2 cut(s) 362, 394
FaqI GGGAC 1 cut(s) 764
FatI CATG 2 cut(s) 708, 844
FauI CCCGC 2 cut(s) 127, 191
Fnu4HI GCNGC 6 cut(s) 17, 558, 677, 680, 683, 1007
FokI GGATG 3 cut(s) 861, 982, 1012
FriOI GRGCYC 2 cut(s) 184, 211
Fsp4HI GCNGC 6 cut(s) 17, 558, 677, 680, 683, 1007
FspBI CTAG 1 cut(s) 104
GluI GCNGC 6 cut(s) 17, 558, 677, 680, 683, 1007
HaeIII GGCC 5 cut(s) 37, 151, 199, 209, 234
HapII CCGG 4 cut(s) 148, 464, 657, 1031
HgaI GACGC 2 cut(s) 242, 1012
Hin1I GRCGYC 1 cut(s) 253
Hin1II CATG 2 cut(s) 712, 848
HinfI GANTC 7 cut(s) 261, 297, 516, 527, 733, 1022, 1064
HpaII CCGG 4 cut(s) 148, 464, 657, 1031
HphI GGTGA 2 cut(s) 149, 155
Hpy166II GTNNAC 1 cut(s) 742
Hpy188I TCNGA 4 cut(s) 296, 651, 835, 918
Hpy188III TCNNGA 2 cut(s) 391, 664
Hpy8I GTNNAC 1 cut(s) 742
Hpy99I CGWCG 5 cut(s) 193, 230, 233, 258, 1006
HpyAV CCTTC 5 cut(s) 27, 349, 364, 388, 977
HpyCH4III ACNGT 2 cut(s) 143, 451
HpyCH4V TGCA 9 cut(s) 19, 47, 101, 434, 524, 539, 551, 797, 924
HpyF10VI GCNNNNNNNGC 5 cut(s) 231, 464, 557, 682, 971
HpyF3I CTNAG 4 cut(s) 153, 242, 293, 621
Hsp92I GRCGYC 1 cut(s) 253
Hsp92II CATG 2 cut(s) 712, 848
Kzo9I GATC 6 cut(s) 387, 397, 587, 653, 826, 1033
LmnI GCTCC 2 cut(s) 321, 694
Lsp1109I GCAGC 4 cut(s) 3, 663, 666, 993
LweI GCATC 1 cut(s) 771
MaeI CTAG 1 cut(s) 104
MaeIII GTNAC 4 cut(s) 137, 143, 730, 838
MalI GATC 6 cut(s) 389, 399, 589, 655, 828, 1035
MbiI CCGCTC 1 cut(s) 186
MboI GATC 6 cut(s) 387, 397, 587, 653, 826, 1033
MboII GAAGA 4 cut(s) 313, 597, 905, 1028
MflI RGATCY 5 cut(s) 397, 587, 653, 826, 1033
MhlI GDGCHC 2 cut(s) 184, 211
MluCI AATT 1 cut(s) 96
MlyI GAGTC 4 cut(s) 255, 521, 727, 1058
MroXI GAANNNNTTC 1 cut(s) 374
MseI TTAA 2 cut(s) 705, 822
MslI CAYNNNNRTG 1 cut(s) 847
MspA1I CMGCKG 1 cut(s) 557
MspCI CTTAAG 1 cut(s) 821
MspI CCGG 4 cut(s) 148, 464, 657, 1031
MspR9I CCNGG 3 cut(s) 250, 455, 1031
Mva1269I GAATGC 1 cut(s) 560
MvaI CCWGG 2 cut(s) 250, 455
MwoI GCNNNNNNNGC 5 cut(s) 231, 464, 557, 682, 971
NciI CCSGG 1 cut(s) 1031
NdeII GATC 6 cut(s) 387, 397, 587, 653, 826, 1033
NlaIII CATG 2 cut(s) 712, 848
NlaIV GGNNCC 4 cut(s) 208, 209, 210, 414
NmuCI GTSAC 3 cut(s) 137, 143, 730
NspI RCATGY 1 cut(s) 712
PceI AGGCCT 2 cut(s) 37, 199
PctI GAATGC 1 cut(s) 560
PdmI GAANNNNTTC 1 cut(s) 374
PfeI GAWTC 3 cut(s) 297, 516, 1022
PkrI GCNGC 6 cut(s) 18, 559, 678, 681, 684, 1008
PleI GAGTC 4 cut(s) 255, 521, 727, 1058
PpsI GAGTC 4 cut(s) 255, 521, 727, 1058
PpuMI RGGWCCY 1 cut(s) 246
PshAI GACNNNNGTC 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 246
Psp6I CCWGG 2 cut(s) 248, 453
PspEI GGTNACC 1 cut(s) 143
PspGI CCWGG 2 cut(s) 248, 453
PspN4I GGNNCC 4 cut(s) 208, 209, 210, 414
PspOMI GGGCCC 1 cut(s) 207
PspPI GGNCC 6 cut(s) 207, 208, 246, 404, 413, 451
PspPPI RGGWCCY 1 cut(s) 246
PstI CTGCAG 1 cut(s) 49
PstNI CAGNNNCTG 1 cut(s) 249
PsuI RGATCY 5 cut(s) 397, 587, 653, 826, 1033
RsaI GTAC 5 cut(s) 71, 423, 474, 814, 888
RsaNI GTAC 5 cut(s) 70, 422, 473, 813, 887
RseI CAYNNNNRTG 1 cut(s) 847
SaqAI TTAA 2 cut(s) 705, 822
SatI GCNGC 6 cut(s) 17, 558, 677, 680, 683, 1007
Sau3AI GATC 6 cut(s) 387, 397, 587, 653, 826, 1033
Sau96I GGNCC 6 cut(s) 207, 208, 246, 404, 413, 451
ScaI AGTACT 1 cut(s) 888
SchI GAGTC 4 cut(s) 255, 521, 727, 1058
ScrFI CCNGG 3 cut(s) 250, 455, 1031
SduI GDGCHC 2 cut(s) 184, 211
SetI ASST 9 cut(s) 18, 78, 159, 248, 409, 493, 550, 634, 723
SfaNI GCATC 1 cut(s) 771
SfcI CTRYAG 1 cut(s) 45
SinI GGWCC 4 cut(s) 246, 404, 413, 451
SmiMI CAYNNNNRTG 1 cut(s) 847
SmlI CTYRAG 2 cut(s) 175, 821
SmoI CTYRAG 2 cut(s) 175, 821
Sse9I AATT 1 cut(s) 96
SseBI AGGCCT 2 cut(s) 37, 199
SsiI CCGC 8 cut(s) 134, 184, 282, 288, 336, 416, 557, 682
SspMI CTAG 1 cut(s) 104
StuI AGGCCT 2 cut(s) 37, 199
StyD4I CCNGG 3 cut(s) 248, 453, 1029
StyI CCWWGG 1 cut(s) 407
TaaI ACNGT 2 cut(s) 143, 451
TaqI TCGA 2 cut(s) 357, 1001
TasI AATT 1 cut(s) 96
TatI WGTACW 3 cut(s) 421, 472, 886
TauI GCSGC 2 cut(s) 560, 685
TfiI GAWTC 3 cut(s) 297, 516, 1022
Tru1I TTAA 2 cut(s) 705, 822
Tru9I TTAA 2 cut(s) 705, 822
TscAI CASTG 2 cut(s) 804, 966
TseFI GTSAC 3 cut(s) 137, 143, 730
TseI GCWGC 4 cut(s) 16, 676, 679, 1006
Tsp45I GTSAC 3 cut(s) 137, 143, 730
TspDTI ATGAA 3 cut(s) 331, 870, 1014
TspGWI ACGGA 1 cut(s) 895
TspRI CASTG 2 cut(s) 804, 966
Vha464I CTTAAG 1 cut(s) 821
VpaK11BI GGWCC 4 cut(s) 246, 404, 413, 451
XceI RCATGY 1 cut(s) 712
XmnI GAANNNNTTC 1 cut(s) 374
XspI CTAG 1 cut(s) 104
ZrmI AGTACT 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.