Rw6G028770

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0017506
Physical Location & Seq
Reverse (-)
52819511 .. 52821525
2015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G028770.1

Sequence Viewer

Length: 636 bp
ATGTGGCTACCTTTTGTGCGATTTTTGTTTTGTTTTGCTTTGCAGCTTGGTGGAGGTTATCTTTTTGGTTTGCCCGTGGGATTCATTGCAGACTCTATCGGTGCAACATTAGGTGCAACAGCAGCATTCATTCTTGGAAGAACAATTGGAAGATCTTATGTTATTTCCAAACTAAAGCATTATCCAAAGTTTCAAGCTGTTGCTGTTGCCATTCAGAGATCTGGTTTCAAGATAGTTCTTCTGCTGCGGCTTGCTCCTTTGCTTCCATTTAACATGCTAAACTACCTACTCTCTGTGACTCCTGTTCACATAGGGGACTATATGCTGGCAACTTGGTTGGGAATGATGCCCATCACATTTGCACTGGTATATGTTGGTACAACTCTTAAGGATCTTTCAGATGTTACACATGGATGGAATGAAGTTTCAACAACTCGTTGGGTGTTTATAGTACTTGGTTTTGTAATATCCGTGATTCTTCTGATTTGCATTAGTAAAGTTGCAAAGGCTTCTTTGGAAAAAGCACTGGCCGACAATGCTGAAATAGAAGGGATATTCTCATCTTCAACGCTGCCCATTGTAGCAGGTTCATCCCCGGATCTTCCAAAAACTCTCATCATACAGATAGACTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

211

Amino Acids

22.94

Weight (kDa)

9.14

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 14 - 127 5.4e-23 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03260
fragaria_vesca FvH4_2g23830
malus_domestica MD03G1267800.v1.1
rosa_chinensis RchiOBHm_Chr6g0291411
rosa_laevigata RLG00000012116
rosa_multiflora Rmu_sc0003735.1_g000021
rosa_roxburghii Rroxscaffold_7G00176070
rosa_rugosa Rorug06G0219100
rosa_samantha Rh6AG331400 Rh6BG338300 Rh6CG344600
rosa_wichuraiana Rw6G028770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 575
AciI CCGC 1 cut(s) 247
AclWI GGATC 2 cut(s) 399, 606
AcoI YGGCCR 1 cut(s) 528
AfaI GTAC 2 cut(s) 379, 453
AflII CTTAAG 1 cut(s) 386
AgsI TTSAA 4 cut(s) 194, 229, 429, 567
AluBI AGCT 2 cut(s) 46, 197
AluI AGCT 2 cut(s) 46, 197
AlwI GGATC 2 cut(s) 399, 606
AoxI GGCC 1 cut(s) 528
ApeKI GCWGC 4 cut(s) 43, 122, 244, 571
AsuC2I CCSGG 1 cut(s) 596
BbvI GCAGC 4 cut(s) 55, 134, 231, 558
BccI CCATC 2 cut(s) 359, 408
BcnI CCSGG 1 cut(s) 596
BfrI CTTAAG 1 cut(s) 386
BfuAI ACCTGC 1 cut(s) 575
BglII AGATCT 2 cut(s) 152, 218
BisI GCNGC 5 cut(s) 44, 123, 245, 248, 572
BlsI GCNGC 5 cut(s) 45, 124, 246, 249, 573
BmcAI AGTACT 1 cut(s) 453
Bme1390I CCNGG 1 cut(s) 596
BmrFI CCNGG 1 cut(s) 596
BmsI GCATC 1 cut(s) 336
BpuMI CCSGG 1 cut(s) 596
BsaBI GATNNNNATC 1 cut(s) 350
BsaJI CCNNGG 2 cut(s) 75, 594
Bse1I ACTGG 2 cut(s) 369, 531
Bse3DI GCAATG 1 cut(s) 84
Bse8I GATNNNNATC 1 cut(s) 350
BseDI CCNNGG 2 cut(s) 75, 594
BseGI GGATG 2 cut(s) 419, 590
BseJI GATNNNNATC 1 cut(s) 350
BseMI GCAATG 1 cut(s) 84
BseNI ACTGG 2 cut(s) 369, 531
BseXI GCAGC 4 cut(s) 55, 134, 231, 558
BshFI GGCC 1 cut(s) 530
BsiSI CCGG 1 cut(s) 596
BslFI GGGAC 1 cut(s) 329
BsmFI GGGAC 1 cut(s) 329
BsmI GAATGC 1 cut(s) 125
BsnI GGCC 1 cut(s) 530
Bsp143I GATC 4 cut(s) 152, 218, 391, 598
BspACI CCGC 1 cut(s) 247
BspANI GGCC 1 cut(s) 530
BspMI ACCTGC 1 cut(s) 575
BspPI GGATC 2 cut(s) 399, 606
BspTI CTTAAG 1 cut(s) 386
BsrDI GCAATG 1 cut(s) 84
BsrI ACTGG 2 cut(s) 369, 531
BssECI CCNNGG 2 cut(s) 75, 594
BssMI GATC 4 cut(s) 152, 218, 391, 598
BstAFI CTTAAG 1 cut(s) 386
BstC8I GCNNGC 2 cut(s) 252, 327
BstDSI CCRYGG 1 cut(s) 75
BstF5I GGATG 2 cut(s) 419, 590
BstKTI GATC 4 cut(s) 155, 221, 394, 601
BstMBI GATC 4 cut(s) 152, 218, 391, 598
BstMWI GCNNNNNNNGC 2 cut(s) 122, 536
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 594
BstV1I GCAGC 4 cut(s) 55, 134, 231, 558
BstX2I RGATCY 4 cut(s) 152, 218, 391, 598
BstYI RGATCY 4 cut(s) 152, 218, 391, 598
BsuRI GGCC 1 cut(s) 530
BtgI CCRYGG 1 cut(s) 75
BtsCI GGATG 2 cut(s) 419, 590
BtsIMutI CAGTG 2 cut(s) 362, 524
BveI ACCTGC 1 cut(s) 575
Cac8I GCNNGC 2 cut(s) 252, 327
Csp6I GTAC 2 cut(s) 378, 452
CviAII CATG 2 cut(s) 274, 410
CviJI RGCY 6 cut(s) 7, 46, 197, 250, 509, 530
CviKI_1 RGCY 6 cut(s) 7, 46, 197, 250, 509, 530
CviQI GTAC 2 cut(s) 378, 452
DpnI GATC 4 cut(s) 154, 220, 393, 600
DpnII GATC 4 cut(s) 152, 218, 391, 598
EaeI YGGCCR 1 cut(s) 528
FaeI CATG 2 cut(s) 277, 413
FaqI GGGAC 1 cut(s) 329
FatI CATG 2 cut(s) 273, 409
Fnu4HI GCNGC 5 cut(s) 44, 123, 245, 248, 572
FokI GGATG 2 cut(s) 426, 577
Fsp4HI GCNGC 5 cut(s) 44, 123, 245, 248, 572
GluI GCNGC 5 cut(s) 44, 123, 245, 248, 572
HaeIII GGCC 1 cut(s) 530
HapII CCGG 1 cut(s) 596
Hin1II CATG 2 cut(s) 277, 413
HinfI GANTC 5 cut(s) 81, 92, 298, 475, 629
HpaII CCGG 1 cut(s) 596
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 3 cut(s) 216, 400, 483
Hpy188III TCNNGA 1 cut(s) 229
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 1 cut(s) 542
HpyCH4V TGCA 7 cut(s) 43, 89, 104, 116, 362, 489, 503
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 536
Hsp92II CATG 2 cut(s) 277, 413
Kzo9I GATC 4 cut(s) 152, 218, 391, 598
LmnI GCTCC 1 cut(s) 259
LpnPI CCDG 7 cut(s) 207, 311, 315, 350, 512, 570, 609
Lsp1109I GCAGC 4 cut(s) 55, 134, 231, 558
LweI GCATC 1 cut(s) 336
MaeIII GTNAC 2 cut(s) 295, 403
MalI GATC 4 cut(s) 154, 220, 393, 600
MboI GATC 4 cut(s) 152, 218, 391, 598
MboII GAAGA 6 cut(s) 150, 162, 230, 470, 555, 593
MfeI CAATTG 1 cut(s) 144
MflI RGATCY 4 cut(s) 152, 218, 391, 598
MluCI AATT 1 cut(s) 144
MlyI GAGTC 3 cut(s) 86, 292, 623
MnlI CCTC 1 cut(s) 47
MseI TTAA 2 cut(s) 270, 387
MslI CAYNNNNRTG 1 cut(s) 412
MspCI CTTAAG 1 cut(s) 386
MspI CCGG 1 cut(s) 596
MspR9I CCNGG 1 cut(s) 596
MunI CAATTG 1 cut(s) 144
Mva1269I GAATGC 1 cut(s) 125
MwoI GCNNNNNNNGC 2 cut(s) 122, 536
NciI CCSGG 1 cut(s) 596
NdeII GATC 4 cut(s) 152, 218, 391, 598
NlaIII CATG 2 cut(s) 277, 413
NmuCI GTSAC 1 cut(s) 295
NspI RCATGY 1 cut(s) 277
PctI GAATGC 1 cut(s) 125
PfeI GAWTC 2 cut(s) 81, 475
PkrI GCNGC 5 cut(s) 45, 124, 246, 249, 573
PleI GAGTC 3 cut(s) 86, 292, 623
PpsI GAGTC 3 cut(s) 86, 292, 623
PsuI RGATCY 4 cut(s) 152, 218, 391, 598
RsaI GTAC 2 cut(s) 379, 453
RsaNI GTAC 2 cut(s) 378, 452
RseI CAYNNNNRTG 1 cut(s) 412
SaqAI TTAA 2 cut(s) 270, 387
SatI GCNGC 5 cut(s) 44, 123, 245, 248, 572
Sau3AI GATC 4 cut(s) 152, 218, 391, 598
ScaI AGTACT 1 cut(s) 453
SchI GAGTC 3 cut(s) 86, 292, 623
ScrFI CCNGG 1 cut(s) 596
SetI ASST 7 cut(s) 13, 48, 58, 115, 199, 288, 589
SfaNI GCATC 1 cut(s) 336
SmiMI CAYNNNNRTG 1 cut(s) 412
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 1 cut(s) 144
SsiI CCGC 1 cut(s) 247
StyD4I CCNGG 1 cut(s) 594
TasI AATT 1 cut(s) 144
TatI WGTACW 1 cut(s) 451
TauI GCSGC 1 cut(s) 250
TfiI GAWTC 2 cut(s) 81, 475
Tru1I TTAA 2 cut(s) 270, 387
Tru9I TTAA 2 cut(s) 270, 387
TscAI CASTG 2 cut(s) 369, 531
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 4 cut(s) 43, 122, 244, 571
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 4 cut(s) 73, 118, 435, 579
TspGWI ACGGA 1 cut(s) 460
TspRI CASTG 2 cut(s) 369, 531
Vha464I CTTAAG 1 cut(s) 386
XceI RCATGY 1 cut(s) 277
ZrmI AGTACT 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.