RchiOBHm_Chr6g0291411

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0017506
Physical Location & Seq
Reverse (-)
54560118 .. 54563150
3033 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26147

Sequence Viewer

Length: 732 bp
ATGTGGTTTGAGCTTAATTCAGGAAGTCTGAAGGATTTCTTGTTATGGATTAAGCAGGATCTTGGACCTTGGGGTCCACTTGTTTTGGCTGTCGCATACGTTCCTCTCACGGTGCTGGCAGTTCCGGCTTCTGTACTTACCCTTGGTGGAGGTTATCTTTTTGGTTTGCCCGTGGGATTCATTGCAGACTCTATCGGTGCAACATTAGGTGCAACAGCAGCATTCATTCTTGGAAGAACAATTGGAAGATCGTATGTTATTTCCAAACTAAAGCATTATCCAAAGTTTCAAGCTGTTGCTGTTGCCATTCAGAGATCTGGTTTCAAGATAGTTCTTCTGCTGCGGCTTGCTCCTTTGCTTCCATTTAACATGCTGAACTACCTACTCTCTGTGACTCCTGTTCACATAGGGGACTATATGCTGGCAACTTGGTTGGGAATGATGCCCATCACATTTGCACTGGTATATGTTGGTACAACGCTTAAGGATCTTTCAGATGTTACACATGGATGGAATGAAGTTTCAACAACTCGTTGGGTGTTTATAGTACTTGGTTTTGGAATATCCGTGATTCTTCTGATTTGCATTAGTAAAGTTGCAAAGGCTGCTTTGGAAAAAGCACTGGCCGACAATGCTGAAATAGAAGGGATATTCTCATCTTCAACGCTGCCCATTGTAGCAGGTTCATCCCCAGATCTTCCAAAAACTCTCATCATACAGATAGACTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

243

Amino Acids

26.13

Weight (kDa)

8.63

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 41 - 159 8.4e-25 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03260
fragaria_vesca FvH4_2g23830
malus_domestica MD03G1267800.v1.1
rosa_chinensis RchiOBHm_Chr6g0291411
rosa_laevigata RLG00000012116
rosa_multiflora Rmu_sc0003735.1_g000021
rosa_roxburghii Rroxscaffold_7G00176070
rosa_rugosa Rorug06G0219100
rosa_samantha Rh6AG331400 Rh6BG338300 Rh6CG344600
rosa_wichuraiana Rw6G028770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 72
Acc36I ACCTGC 1 cut(s) 671
AciI CCGC 1 cut(s) 343
AclWI GGATC 2 cut(s) 66, 495
AcoI YGGCCR 1 cut(s) 624
AcuI CTGAAG 1 cut(s) 50
AfaI GTAC 3 cut(s) 135, 475, 549
AflII CTTAAG 1 cut(s) 482
AgsI TTSAA 4 cut(s) 290, 325, 525, 663
AluBI AGCT 2 cut(s) 13, 293
AluI AGCT 2 cut(s) 13, 293
AlwI GGATC 2 cut(s) 66, 495
AoxI GGCC 1 cut(s) 624
ApeKI GCWGC 4 cut(s) 218, 340, 605, 667
Asp700I GAANNNNTTC 1 cut(s) 35
AspS9I GGNCC 2 cut(s) 65, 74
AvaII GGWCC 2 cut(s) 65, 74
BbvI GCAGC 4 cut(s) 230, 327, 592, 654
BccI CCATC 2 cut(s) 455, 504
BfrI CTTAAG 1 cut(s) 482
BfuAI ACCTGC 1 cut(s) 671
BglII AGATCT 2 cut(s) 314, 694
BisI GCNGC 5 cut(s) 219, 341, 344, 606, 668
BlsI GCNGC 5 cut(s) 220, 342, 345, 607, 669
BmcAI AGTACT 1 cut(s) 549
Bme18I GGWCC 2 cut(s) 65, 74
BmgT120I GGNCC 2 cut(s) 65, 74
BmiI GGNNCC 1 cut(s) 75
BmsI GCATC 1 cut(s) 432
BsaBI GATNNNNATC 1 cut(s) 446
BsaJI CCNNGG 3 cut(s) 68, 142, 171
Bse1I ACTGG 2 cut(s) 465, 627
Bse3DI GCAATG 1 cut(s) 180
Bse8I GATNNNNATC 1 cut(s) 446
BseDI CCNNGG 3 cut(s) 68, 142, 171
BseGI GGATG 2 cut(s) 515, 686
BseJI GATNNNNATC 1 cut(s) 446
BseMI GCAATG 1 cut(s) 180
BseNI ACTGG 2 cut(s) 465, 627
BseXI GCAGC 4 cut(s) 230, 327, 592, 654
BshFI GGCC 1 cut(s) 626
BsiSI CCGG 1 cut(s) 125
BslFI GGGAC 1 cut(s) 425
BsmFI GGGAC 1 cut(s) 425
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 1 cut(s) 626
Bsp143I GATC 5 cut(s) 58, 248, 314, 487, 694
BspACI CCGC 1 cut(s) 343
BspANI GGCC 1 cut(s) 626
BspLI GGNNCC 1 cut(s) 75
BspMI ACCTGC 1 cut(s) 671
BspPI GGATC 2 cut(s) 66, 495
BspTI CTTAAG 1 cut(s) 482
BsrDI GCAATG 1 cut(s) 180
BsrI ACTGG 2 cut(s) 465, 627
BssECI CCNNGG 3 cut(s) 68, 142, 171
BssMI GATC 5 cut(s) 58, 248, 314, 487, 694
BssT1I CCWWGG 2 cut(s) 68, 142
Bst4CI ACNGT 1 cut(s) 112
BstAFI CTTAAG 1 cut(s) 482
BstAPI GCANNNNNTGC 1 cut(s) 605
BstC8I GCNNGC 3 cut(s) 117, 348, 423
BstDSI CCRYGG 1 cut(s) 171
BstF5I GGATG 2 cut(s) 515, 686
BstKTI GATC 5 cut(s) 61, 251, 317, 490, 697
BstMBI GATC 5 cut(s) 58, 248, 314, 487, 694
BstMWI GCNNNNNNNGC 4 cut(s) 125, 218, 605, 632
BstNSI RCATGY 1 cut(s) 373
BstV1I GCAGC 4 cut(s) 230, 327, 592, 654
BstX2I RGATCY 4 cut(s) 58, 314, 487, 694
BstYI RGATCY 4 cut(s) 58, 314, 487, 694
BsuRI GGCC 1 cut(s) 626
BtgI CCRYGG 1 cut(s) 171
BtsCI GGATG 2 cut(s) 515, 686
BtsIMutI CAGTG 2 cut(s) 458, 620
BveI ACCTGC 1 cut(s) 671
Cac8I GCNNGC 3 cut(s) 117, 348, 423
Cfr13I GGNCC 2 cut(s) 65, 74
Csp6I GTAC 3 cut(s) 134, 474, 548
CspCI CAANNNNNGTGG 2 cut(s) 66, 101
CviAII CATG 2 cut(s) 370, 506
CviJI RGCY 7 cut(s) 13, 89, 128, 293, 346, 605, 626
CviKI_1 RGCY 7 cut(s) 13, 89, 128, 293, 346, 605, 626
CviQI GTAC 3 cut(s) 134, 474, 548
DpnI GATC 5 cut(s) 60, 250, 316, 489, 696
DpnII GATC 5 cut(s) 58, 248, 314, 487, 694
DrdI GACNNNNNNGTC 1 cut(s) 72
DseDI GACNNNNNNGTC 1 cut(s) 72
EaeI YGGCCR 1 cut(s) 624
Eco130I CCWWGG 2 cut(s) 68, 142
Eco47I GGWCC 2 cut(s) 65, 74
Eco57I CTGAAG 1 cut(s) 50
EcoT14I CCWWGG 2 cut(s) 68, 142
ErhI CCWWGG 2 cut(s) 68, 142
FaeI CATG 2 cut(s) 373, 509
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FaqI GGGAC 1 cut(s) 425
FatI CATG 2 cut(s) 369, 505
Fnu4HI GCNGC 5 cut(s) 219, 341, 344, 606, 668
FokI GGATG 2 cut(s) 522, 673
Fsp4HI GCNGC 5 cut(s) 219, 341, 344, 606, 668
GluI GCNGC 5 cut(s) 219, 341, 344, 606, 668
HaeIII GGCC 1 cut(s) 626
HapII CCGG 1 cut(s) 125
Hin1II CATG 2 cut(s) 373, 509
HinfI GANTC 5 cut(s) 177, 188, 394, 571, 725
HpaII CCGG 1 cut(s) 125
Hpy166II GTNNAC 2 cut(s) 77, 403
Hpy188I TCNGA 4 cut(s) 30, 312, 496, 579
Hpy188III TCNNGA 2 cut(s) 21, 325
Hpy8I GTNNAC 2 cut(s) 77, 403
HpyAV CCTTC 2 cut(s) 25, 638
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 6 cut(s) 185, 200, 212, 458, 585, 599
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 218, 605, 632
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 2 cut(s) 373, 509
Kzo9I GATC 5 cut(s) 58, 248, 314, 487, 694
LmnI GCTCC 1 cut(s) 355
Lsp1109I GCAGC 4 cut(s) 230, 327, 592, 654
LweI GCATC 1 cut(s) 432
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 2 cut(s) 391, 499
MalI GATC 5 cut(s) 60, 250, 316, 489, 696
MboI GATC 5 cut(s) 58, 248, 314, 487, 694
MboII GAAGA 6 cut(s) 246, 258, 326, 566, 651, 689
MfeI CAATTG 1 cut(s) 240
MflI RGATCY 4 cut(s) 58, 314, 487, 694
MluCI AATT 2 cut(s) 16, 240
MlyI GAGTC 3 cut(s) 182, 388, 719
MnlI CCTC 2 cut(s) 114, 143
MroXI GAANNNNTTC 1 cut(s) 35
MseI TTAA 4 cut(s) 15, 51, 366, 483
MslI CAYNNNNRTG 1 cut(s) 508
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 1 cut(s) 125
MunI CAATTG 1 cut(s) 240
Mva1269I GAATGC 1 cut(s) 221
MwoI GCNNNNNNNGC 4 cut(s) 125, 218, 605, 632
NdeII GATC 5 cut(s) 58, 248, 314, 487, 694
NlaIII CATG 2 cut(s) 373, 509
NlaIV GGNNCC 1 cut(s) 75
NmuCI GTSAC 1 cut(s) 391
NspI RCATGY 1 cut(s) 373
PctI GAATGC 1 cut(s) 221
PdmI GAANNNNTTC 1 cut(s) 35
PfeI GAWTC 2 cut(s) 177, 571
PkrI GCNGC 5 cut(s) 220, 342, 345, 607, 669
PleI GAGTC 3 cut(s) 182, 388, 719
PpsI GAGTC 3 cut(s) 182, 388, 719
PspN4I GGNNCC 1 cut(s) 75
PspPI GGNCC 2 cut(s) 65, 74
PsuI RGATCY 4 cut(s) 58, 314, 487, 694
RsaI GTAC 3 cut(s) 135, 475, 549
RsaNI GTAC 3 cut(s) 134, 474, 548
RseI CAYNNNNRTG 1 cut(s) 508
SaqAI TTAA 4 cut(s) 15, 51, 366, 483
SatI GCNGC 5 cut(s) 219, 341, 344, 606, 668
Sau3AI GATC 5 cut(s) 58, 248, 314, 487, 694
Sau96I GGNCC 2 cut(s) 65, 74
ScaI AGTACT 1 cut(s) 549
SchI GAGTC 3 cut(s) 182, 388, 719
SetI ASST 8 cut(s) 15, 70, 102, 154, 211, 295, 384, 685
SfaNI GCATC 1 cut(s) 432
SinI GGWCC 2 cut(s) 65, 74
SmiMI CAYNNNNRTG 1 cut(s) 508
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
Sse9I AATT 2 cut(s) 16, 240
SsiI CCGC 1 cut(s) 343
StyI CCWWGG 2 cut(s) 68, 142
TaaI ACNGT 1 cut(s) 112
TaiI ACGT 1 cut(s) 102
TasI AATT 2 cut(s) 16, 240
TatI WGTACW 2 cut(s) 133, 547
TauI GCSGC 1 cut(s) 346
TfiI GAWTC 2 cut(s) 177, 571
Tru1I TTAA 4 cut(s) 15, 51, 366, 483
Tru9I TTAA 4 cut(s) 15, 51, 366, 483
TscAI CASTG 2 cut(s) 465, 627
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 4 cut(s) 218, 340, 605, 667
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 4 cut(s) 169, 214, 531, 675
TspGWI ACGGA 1 cut(s) 556
TspRI CASTG 2 cut(s) 465, 627
Vha464I CTTAAG 1 cut(s) 482
VpaK11BI GGWCC 2 cut(s) 65, 74
XceI RCATGY 1 cut(s) 373
XmnI GAANNNNTTC 1 cut(s) 35
ZrmI AGTACT 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.