Rh6AG331400

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0017506
Physical Location & Seq
Reverse (-)
53271388 .. 53275314
3927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG331400.1

Sequence Viewer

Length: 960 bp
ATGGAAAGCGGTGTTTGGCGTCGTGCTGCTGGAGATAGATCACTGAATGATGAGCAAAGGGCCCACAACTTTTCTAGGCTGGAGACGATGTGCGATAGCGTGGTGAAGAAGGGTCCAAAGGCTAGGATCCGGGTCGGGTCTGGATTTGCGCTTGGGGTTGGGTCCGGATTTGGGATCCTGCTACTAAATCTGGCAAACTTGAGAGCCCGCTCGTCGTTTAGGCCTGATGGGGCCCGCAGCTTCGACGGCGACGACGACCGTCATCTGAGATCTTGGCGTCGCAAACTCCTCTCCTCCCTCACCGCCGTCAAACTCAAGCTCACCTGGACCTCCGCCTTCAGATTCGCCCTCCTCCTCTTCCTCCTCGCCGCCATTGCCACCGCCTGCATTTCCCTCCCAATCGAAAAGAGTTTGAAGGATTTCTTGTTATGGATTAAGCAGGATCTTGGACCTTGGGGTCCACTTGTTTTGGCTGTCGCATACGTTCCTCTCACGGCGCTGGCAGTTCCGGCTTCTGTACTTACCCTTGGTGGAGGTTATCTTTTTGGTTTGCCCGTGGGATTCATTGCAGACTCTATCGGTGCAACATTAGGTGCAACAGCAGCATTCATTCTTGGAAGAACAATTGGAAGATCTTTTGTTATTTCCAAACTAAAGCATTATCCAAAGTTTCAAGCTGTTGCTGTTGCCATTCAGAGATCTGGTTTCAAGATAGTTCTTCTGCTGCGGCTTGCTCCTTTGCTTCCATTTAACATGCTGAACTACCTACTCTCTGTGACTCCTGTTCACATAGGGGACTATATGCTGGCAACTTGGTTGGGAATGATGCCCATCACATTTGCACTGGTATATGTTGGTACAACTCTTAAGGATCTTTCAGATGTTACACATGGATGGAATGAAGTTTCAACAACTCGTTGGGTGTTTATAGTACTTGGTTTTGGAATATCCGGTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

319

Amino Acids

34.78

Weight (kDa)

10.16

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 169 - 287 2.6e-24 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03260
fragaria_vesca FvH4_2g23830
malus_domestica MD03G1267800.v1.1
rosa_chinensis RchiOBHm_Chr6g0291411
rosa_laevigata RLG00000012116
rosa_multiflora Rmu_sc0003735.1_g000021
rosa_roxburghii Rroxscaffold_7G00176070
rosa_rugosa Rorug06G0219100
rosa_samantha Rh6AG331400 Rh6BG338300 Rh6CG344600
rosa_wichuraiana Rw6G028770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 456
AccBSI CCGCTC 1 cut(s) 210
AccIII TCCGGA 1 cut(s) 164
AciI CCGC 8 cut(s) 9, 208, 235, 303, 333, 369, 381, 727
AclWI GGATC 6 cut(s) 121, 134, 169, 182, 450, 879
AcuI CTGAAG 1 cut(s) 322
AcyI GRCGYC 2 cut(s) 19, 277
AfaI GTAC 3 cut(s) 519, 859, 933
AfiI CCNNNNNNNGG 1 cut(s) 171
AflII CTTAAG 1 cut(s) 866
AgsI TTSAA 4 cut(s) 415, 674, 709, 909
AjnI CCWGG 1 cut(s) 323
AluBI AGCT 3 cut(s) 240, 319, 677
AluI AGCT 3 cut(s) 240, 319, 677
Alw26I GTCTC 1 cut(s) 77
AlwI GGATC 6 cut(s) 121, 134, 169, 182, 450, 879
Aor13HI TCCGGA 1 cut(s) 164
AoxI GGCC 3 cut(s) 60, 221, 231
ApaI GGGCCC 2 cut(s) 64, 235
ApeKI GCWGC 4 cut(s) 26, 237, 602, 724
Asp700I GAANNNNTTC 1 cut(s) 419
AspLEI GCGC 2 cut(s) 151, 499
AspS9I GGNCC 9 cut(s) 60, 61, 113, 162, 231, 232, 327, 449, 458
AsuC2I CCSGG 1 cut(s) 131
AsuHPI GGTGA 3 cut(s) 115, 292, 313
AvaII GGWCC 5 cut(s) 113, 162, 327, 449, 458
BaeGI GKGCMC 2 cut(s) 64, 235
BamHI GGATCC 2 cut(s) 126, 174
BanII GRGCYC 3 cut(s) 64, 208, 235
BbvI GCAGC 4 cut(s) 13, 249, 614, 711
BccI CCATC 3 cut(s) 221, 839, 888
BceAI ACGGC 3 cut(s) 262, 290, 510
BciT130I CCWGG 1 cut(s) 325
BcnI CCSGG 1 cut(s) 131
BcoDI GTCTC 1 cut(s) 77
BfaI CTAG 2 cut(s) 75, 123
BfoI RGCGCY 1 cut(s) 500
BfrI CTTAAG 1 cut(s) 866
BglII AGATCT 3 cut(s) 269, 632, 698
BisI GCNGC 6 cut(s) 27, 238, 369, 603, 725, 728
BlsI GCNGC 6 cut(s) 28, 239, 370, 604, 726, 729
BmcAI AGTACT 1 cut(s) 933
Bme1390I CCNGG 2 cut(s) 131, 325
Bme18I GGWCC 5 cut(s) 113, 162, 327, 449, 458
BmgT120I GGNCC 9 cut(s) 60, 61, 113, 162, 231, 232, 327, 449, 458
BmiI GGNNCC 8 cut(s) 62, 114, 128, 163, 176, 232, 233, 459
BmrFI CCNGG 2 cut(s) 131, 325
BmsI GCATC 1 cut(s) 816
BoxI GACNNNNGTC 1 cut(s) 258
BpmI CTGGAG 2 cut(s) 51, 101
BpuEI CTTGAG 2 cut(s) 220, 299
BpuMI CCSGG 1 cut(s) 131
BsaBI GATNNNNATC 1 cut(s) 830
BsaHI GRCGYC 2 cut(s) 19, 277
BsaJI CCNNGG 3 cut(s) 452, 526, 555
BsaWI WCCGGW 2 cut(s) 164, 950
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 1 cut(s) 171
Bse1I ACTGG 1 cut(s) 849
Bse3DI GCAATG 2 cut(s) 372, 564
Bse8I GATNNNNATC 1 cut(s) 830
BseAI TCCGGA 1 cut(s) 164
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 3 cut(s) 452, 526, 555
BseGI GGATG 1 cut(s) 899
BseJI GATNNNNATC 1 cut(s) 830
BseLI CCNNNNNNNGG 1 cut(s) 171
BseMI GCAATG 2 cut(s) 372, 564
BseMII CTCAG 1 cut(s) 257
BseNI ACTGG 1 cut(s) 849
BseRI GAGGAG 5 cut(s) 278, 283, 341, 344, 353
BseSI GKGCMC 2 cut(s) 64, 235
BseXI GCAGC 4 cut(s) 13, 249, 614, 711
Bsh1285I CGRYCG 1 cut(s) 259
BshFI GGCC 3 cut(s) 62, 223, 233
BsiEI CGRYCG 1 cut(s) 259
BsiSI CCGG 4 cut(s) 130, 165, 509, 951
BslFI GGGAC 1 cut(s) 809
BslI CCNNNNNNNGG 1 cut(s) 171
BsmAI GTCTC 1 cut(s) 77
BsmBI CGTCTC 1 cut(s) 77
BsmFI GGGAC 1 cut(s) 809
BsmI GAATGC 1 cut(s) 605
BsnI GGCC 3 cut(s) 62, 223, 233
Bsp120I GGGCCC 2 cut(s) 60, 231
Bsp1286I GDGCHC 3 cut(s) 64, 208, 235
Bsp13I TCCGGA 1 cut(s) 164
Bsp143I GATC 8 cut(s) 38, 126, 174, 269, 442, 632, 698, 871
BspACI CCGC 8 cut(s) 9, 208, 235, 303, 333, 369, 381, 727
BspANI GGCC 3 cut(s) 62, 223, 233
BspCNI CTCAG 1 cut(s) 258
BspEI TCCGGA 1 cut(s) 164
BspLI GGNNCC 8 cut(s) 62, 114, 128, 163, 176, 232, 233, 459
BspPI GGATC 6 cut(s) 121, 134, 169, 182, 450, 879
BspTI CTTAAG 1 cut(s) 866
BsrBI CCGCTC 1 cut(s) 210
BsrDI GCAATG 2 cut(s) 372, 564
BsrI ACTGG 1 cut(s) 849
BssECI CCNNGG 3 cut(s) 452, 526, 555
BssMI GATC 8 cut(s) 38, 126, 174, 269, 442, 632, 698, 871
BssNI GRCGYC 2 cut(s) 19, 277
BssT1I CCWWGG 2 cut(s) 452, 526
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 1 cut(s) 260
Bst6I CTCTTC 1 cut(s) 362
BstACI GRCGYC 2 cut(s) 19, 277
BstAFI CTTAAG 1 cut(s) 866
BstC8I GCNNGC 6 cut(s) 208, 235, 385, 501, 732, 807
BstDEI CTNAG 1 cut(s) 266
BstDSI CCRYGG 1 cut(s) 555
BstF5I GGATG 1 cut(s) 899
BstH2I RGCGCY 1 cut(s) 500
BstHHI GCGC 2 cut(s) 151, 499
BstKTI GATC 8 cut(s) 41, 129, 177, 272, 445, 635, 701, 874
BstMAI GTCTC 1 cut(s) 77
BstMBI GATC 8 cut(s) 38, 126, 174, 269, 442, 632, 698, 871
BstMCI CGRYCG 1 cut(s) 259
BstMWI GCNNNNNNNGC 4 cut(s) 246, 374, 509, 602
BstNI CCWGG 1 cut(s) 325
BstNSI RCATGY 1 cut(s) 757
BstPAI GACNNNNGTC 1 cut(s) 258
BstSCI CCNGG 2 cut(s) 129, 323
BstSLI GKGCMC 2 cut(s) 64, 235
BstV1I GCAGC 4 cut(s) 13, 249, 614, 711
BstX2I RGATCY 7 cut(s) 126, 174, 269, 442, 632, 698, 871
BstYI RGATCY 7 cut(s) 126, 174, 269, 442, 632, 698, 871
BsuRI GGCC 3 cut(s) 62, 223, 233
BtgI CCRYGG 1 cut(s) 555
BtsCI GGATG 1 cut(s) 899
BtsIMutI CAGTG 2 cut(s) 41, 842
Cac8I GCNNGC 6 cut(s) 208, 235, 385, 501, 732, 807
CfoI GCGC 2 cut(s) 151, 499
Cfr13I GGNCC 9 cut(s) 60, 61, 113, 162, 231, 232, 327, 449, 458
CseI GACGC 2 cut(s) 8, 266
Csp6I GTAC 3 cut(s) 518, 858, 932
CspCI CAANNNNNGTGG 2 cut(s) 450, 485
CviAII CATG 2 cut(s) 754, 890
CviQI GTAC 3 cut(s) 518, 858, 932
DdeI CTNAG 1 cut(s) 266
DpnI GATC 8 cut(s) 40, 128, 176, 271, 444, 634, 700, 873
DpnII GATC 8 cut(s) 38, 126, 174, 269, 442, 632, 698, 871
DrdI GACNNNNNNGTC 1 cut(s) 456
DseDI GACNNNNNNGTC 1 cut(s) 456
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
EciI GGCGGA 1 cut(s) 322
Eco130I CCWWGG 2 cut(s) 452, 526
Eco147I AGGCCT 1 cut(s) 223
Eco24I GRGCYC 3 cut(s) 64, 208, 235
Eco47I GGWCC 5 cut(s) 113, 162, 327, 449, 458
Eco57I CTGAAG 1 cut(s) 322
EcoO109I RGGNCCY 2 cut(s) 60, 231
EcoRII CCWGG 1 cut(s) 323
EcoT14I CCWWGG 2 cut(s) 452, 526
EcoT38I GRGCYC 3 cut(s) 64, 208, 235
ErhI CCWWGG 2 cut(s) 452, 526
Esp3I CGTCTC 1 cut(s) 77
FaeI CATG 2 cut(s) 757, 893
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FaqI GGGAC 1 cut(s) 809
FatI CATG 2 cut(s) 753, 889
FauI CCCGC 2 cut(s) 215, 242
Fnu4HI GCNGC 6 cut(s) 27, 238, 369, 603, 725, 728
FokI GGATG 1 cut(s) 906
FriOI GRGCYC 3 cut(s) 64, 208, 235
Fsp4HI GCNGC 6 cut(s) 27, 238, 369, 603, 725, 728
FspBI CTAG 2 cut(s) 75, 123
GlaI GCGC 2 cut(s) 150, 498
GluI GCNGC 6 cut(s) 27, 238, 369, 603, 725, 728
GsuI CTGGAG 2 cut(s) 51, 101
HaeII RGCGCY 1 cut(s) 500
HaeIII GGCC 3 cut(s) 62, 223, 233
HapII CCGG 4 cut(s) 130, 165, 509, 951
HgaI GACGC 2 cut(s) 8, 266
HhaI GCGC 2 cut(s) 151, 499
Hin1I GRCGYC 2 cut(s) 19, 277
Hin1II CATG 2 cut(s) 757, 893
Hin6I GCGC 2 cut(s) 149, 497
HinP1I GCGC 2 cut(s) 149, 497
HinfI GANTC 4 cut(s) 342, 561, 572, 778
HpaII CCGG 4 cut(s) 130, 165, 509, 951
HphI GGTGA 3 cut(s) 115, 292, 313
Hpy166II GTNNAC 2 cut(s) 461, 787
Hpy188I TCNGA 4 cut(s) 267, 341, 696, 880
Hpy188III TCNNGA 3 cut(s) 141, 165, 709
Hpy8I GTNNAC 2 cut(s) 461, 787
Hpy99I CGWCG 6 cut(s) 24, 217, 248, 254, 257, 282
HpyAV CCTTC 3 cut(s) 103, 346, 409
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4IV ACGT 1 cut(s) 483
HpyCH4V TGCA 5 cut(s) 387, 569, 584, 596, 842
HpyF10VI GCNNNNNNNGC 4 cut(s) 246, 374, 509, 602
HpyF3I CTNAG 1 cut(s) 266
HpySE526I ACGT 1 cut(s) 483
Hsp92I GRCGYC 2 cut(s) 19, 277
Hsp92II CATG 2 cut(s) 757, 893
HspAI GCGC 2 cut(s) 149, 497
Kpn2I TCCGGA 1 cut(s) 164
Kzo9I GATC 8 cut(s) 38, 126, 174, 269, 442, 632, 698, 871
LmnI GCTCC 1 cut(s) 739
Lsp1109I GCAGC 4 cut(s) 13, 249, 614, 711
LweI GCATC 1 cut(s) 816
MaeI CTAG 2 cut(s) 75, 123
MaeII ACGT 1 cut(s) 483
MaeIII GTNAC 2 cut(s) 775, 883
MalI GATC 8 cut(s) 40, 128, 176, 271, 444, 634, 700, 873
MbiI CCGCTC 1 cut(s) 210
MboI GATC 8 cut(s) 38, 126, 174, 269, 442, 632, 698, 871
MboII GAAGA 5 cut(s) 118, 349, 630, 642, 710
MfeI CAATTG 1 cut(s) 624
MflI RGATCY 7 cut(s) 126, 174, 269, 442, 632, 698, 871
MhlI GDGCHC 3 cut(s) 64, 208, 235
MluCI AATT 1 cut(s) 624
MlyI GAGTC 2 cut(s) 566, 772
MroI TCCGGA 1 cut(s) 164
MroXI GAANNNNTTC 1 cut(s) 419
MseI TTAA 3 cut(s) 435, 750, 867
MslI CAYNNNNRTG 1 cut(s) 892
MspCI CTTAAG 1 cut(s) 866
MspI CCGG 4 cut(s) 130, 165, 509, 951
MspR9I CCNGG 2 cut(s) 131, 325
MunI CAATTG 1 cut(s) 624
Mva1269I GAATGC 1 cut(s) 605
MvaI CCWGG 1 cut(s) 325
MwoI GCNNNNNNNGC 4 cut(s) 246, 374, 509, 602
NciI CCSGG 1 cut(s) 131
NdeII GATC 8 cut(s) 38, 126, 174, 269, 442, 632, 698, 871
NlaIII CATG 2 cut(s) 757, 893
NlaIV GGNNCC 8 cut(s) 62, 114, 128, 163, 176, 232, 233, 459
NmuCI GTSAC 1 cut(s) 775
NspI RCATGY 1 cut(s) 757
PceI AGGCCT 1 cut(s) 223
PcsI WCGNNNNNNNCGW 2 cut(s) 249, 252
PctI GAATGC 1 cut(s) 605
PdmI GAANNNNTTC 1 cut(s) 419
PfeI GAWTC 2 cut(s) 342, 561
PkrI GCNGC 6 cut(s) 28, 239, 370, 604, 726, 729
PleI GAGTC 2 cut(s) 566, 772
PpsI GAGTC 2 cut(s) 566, 772
PshAI GACNNNNGTC 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 323
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 8 cut(s) 62, 114, 128, 163, 176, 232, 233, 459
PspOMI GGGCCC 2 cut(s) 60, 231
PspPI GGNCC 9 cut(s) 60, 61, 113, 162, 231, 232, 327, 449, 458
PsuI RGATCY 7 cut(s) 126, 174, 269, 442, 632, 698, 871
RsaI GTAC 3 cut(s) 519, 859, 933
RsaNI GTAC 3 cut(s) 518, 858, 932
RseI CAYNNNNRTG 1 cut(s) 892
SaqAI TTAA 3 cut(s) 435, 750, 867
SatI GCNGC 6 cut(s) 27, 238, 369, 603, 725, 728
Sau3AI GATC 8 cut(s) 38, 126, 174, 269, 442, 632, 698, 871
Sau96I GGNCC 9 cut(s) 60, 61, 113, 162, 231, 232, 327, 449, 458
ScaI AGTACT 1 cut(s) 933
SchI GAGTC 2 cut(s) 566, 772
ScrFI CCNGG 2 cut(s) 131, 325
SduI GDGCHC 3 cut(s) 64, 208, 235
SfaNI GCATC 1 cut(s) 816
SinI GGWCC 5 cut(s) 113, 162, 327, 449, 458
SmiMI CAYNNNNRTG 1 cut(s) 892
SmlI CTYRAG 3 cut(s) 199, 314, 866
SmoI CTYRAG 3 cut(s) 199, 314, 866
Sse9I AATT 1 cut(s) 624
SseBI AGGCCT 1 cut(s) 223
SsiI CCGC 8 cut(s) 9, 208, 235, 303, 333, 369, 381, 727
SspMI CTAG 2 cut(s) 75, 123
StuI AGGCCT 1 cut(s) 223
StyD4I CCNGG 2 cut(s) 129, 323
StyI CCWWGG 2 cut(s) 452, 526
TaaI ACNGT 1 cut(s) 260
TaiI ACGT 1 cut(s) 486
TaqI TCGA 2 cut(s) 243, 402
TasI AATT 1 cut(s) 624
TatI WGTACW 2 cut(s) 517, 931
TauI GCSGC 2 cut(s) 371, 730
TfiI GAWTC 2 cut(s) 342, 561
Tru1I TTAA 3 cut(s) 435, 750, 867
Tru9I TTAA 3 cut(s) 435, 750, 867
TscAI CASTG 2 cut(s) 48, 849
TseFI GTSAC 1 cut(s) 775
TseI GCWGC 4 cut(s) 26, 237, 602, 724
Tsp45I GTSAC 1 cut(s) 775
TspDTI ATGAA 3 cut(s) 553, 598, 915
TspRI CASTG 2 cut(s) 48, 849
Vha464I CTTAAG 1 cut(s) 866
VpaK11BI GGWCC 5 cut(s) 113, 162, 327, 449, 458
XceI RCATGY 1 cut(s) 757
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 2 cut(s) 75, 123
ZrmI AGTACT 1 cut(s) 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.