MD03G1267800.v1.1

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0017506
Physical Location & Seq
Reverse (-)
35271562 .. 35274466
2905 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1267800.v1.1.491

Sequence Viewer

Length: 804 bp
ATGGCACTCCGCTACCTCTCCTGGGCTTGCGCCTTCCGATCCGCCGTCTTCCTCTTCCTCCTCGCCGCCGTCGTCACCGCCTGCTTTTTGAAGGACTTCTTGATATGGGTTAAGCAGGATCTTGGCCCCTGGGGTCCACTTGTGCTGGCTGTTGCATACATTCCTCTCACAGTCCTTTCTGTTCCAGCTTCTGTGCTTACACTTGGTGGAGGTTATCTTTTTGGCTTGCCTGTTGGATTCGTTGCCGACTCTGTTGGTGCAACATTAGGTGCCACGGCGTCATTTTTTCTTGGAAGAACAATTGGAAGATCTTATGTCACTTCCAAACTCAAGAATTATCCAAAGTTTCAAGCTGTTTCTATTGCAATTCACAGATCTGGTTTTAAGATAGTTCTGCTACTTCGGCTTGCTCCTTTGGTTCCATTTAACATGCTGAACTACCTCCTCTCGGTGACTCCTGTTAACTTAGGGGACTATGTACTGGCATCTTGGTTGGGGATGATGCCCACCACATTTGCACTGGTGTATGTTGGTACAACACTTAAGGATCTTTCAGACGTTACAGACGGGTGGAATGGGGTTTCAACCACTCACTGGGTGTTTATAGCCCTAGGTTTTGGAATATCCGTGATTCTAATGGCTTGCATTACTAAAGTTGCAAAGGCTTCATTGGACAAAGCAGTGGCTGAAAATGCTGAAATAGAAGGCATCTTGTCCCCTTCAATGCTATCCATCGGAGCAGATTCGTCCTTGGATCTTCTGAAGCCTCTCATAGTCCGGATAGACTCAGACCGGTTAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

268

Amino Acids

28.7

Weight (kDa)

9.13

Isoelectric Point (pI)

31.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 61 - 179 7.5e-26 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03260
fragaria_vesca FvH4_2g23830
malus_domestica MD03G1267800.v1.1
rosa_chinensis RchiOBHm_Chr6g0291411
rosa_laevigata RLG00000012116
rosa_multiflora Rmu_sc0003735.1_g000021
rosa_roxburghii Rroxscaffold_7G00176070
rosa_rugosa Rorug06G0219100
rosa_samantha Rh6AG331400 Rh6BG338300 Rh6CG344600
rosa_wichuraiana Rw6G028770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 269
AccB7I CCANNNNNTGG 1 cut(s) 594
AccIII TCCGGA 1 cut(s) 777
AciI CCGC 4 cut(s) 10, 42, 66, 78
AclWI GGATC 4 cut(s) 33, 126, 555, 762
AcuI CTGAAG 1 cut(s) 782
AcyI GRCGYC 1 cut(s) 278
AdeI CACNNNGTG 2 cut(s) 206, 598
AfaI GTAC 2 cut(s) 480, 535
AfiI CCNNNNNNNGG 3 cut(s) 22, 448, 594
AflII CTTAAG 1 cut(s) 542
AgeI ACCGGT 1 cut(s) 792
AgsI TTSAA 4 cut(s) 91, 350, 585, 723
AjnI CCWGG 2 cut(s) 20, 128
AluBI AGCT 2 cut(s) 188, 353
AluI AGCT 2 cut(s) 188, 353
AlwI GGATC 4 cut(s) 33, 126, 555, 762
AlwNI CAGNNNCTG 2 cut(s) 191, 686
Aor13HI TCCGGA 1 cut(s) 777
AoxI GGCC 1 cut(s) 124
AsiGI ACCGGT 1 cut(s) 792
Asp700I GAANNNNTTC 1 cut(s) 95
AspA2I CCTAGG 1 cut(s) 610
AspLEI GCGC 1 cut(s) 32
AspS9I GGNCC 2 cut(s) 125, 134
AsuHPI GGTGA 2 cut(s) 67, 463
AvaII GGWCC 1 cut(s) 134
AvrII CCTAGG 1 cut(s) 610
BanI GGYRCC 1 cut(s) 269
BbsI GAAGAC 1 cut(s) 40
BccI CCATC 1 cut(s) 740
BceAI ACGGC 3 cut(s) 29, 53, 291
BciT130I CCWGG 2 cut(s) 22, 130
BfaI CTAG 1 cut(s) 611
BfrI CTTAAG 1 cut(s) 542
BglII AGATCT 2 cut(s) 308, 374
BisI GCNGC 1 cut(s) 66
BlnI CCTAGG 1 cut(s) 610
BlsI GCNGC 1 cut(s) 67
Bme1390I CCNGG 2 cut(s) 22, 130
Bme18I GGWCC 1 cut(s) 134
BmgT120I GGNCC 2 cut(s) 125, 134
BmiI GGNNCC 4 cut(s) 127, 135, 271, 420
BmrFI CCNGG 2 cut(s) 22, 130
BmrI ACTGGG 1 cut(s) 604
BmsI GCATC 3 cut(s) 492, 494, 717
BmuI ACTGGG 1 cut(s) 604
BpiI GAAGAC 1 cut(s) 40
BpuEI CTTGAG 1 cut(s) 314
BsaHI GRCGYC 1 cut(s) 278
BsaJI CCNNGG 6 cut(s) 21, 128, 129, 273, 610, 750
BsaWI WCCGGW 2 cut(s) 777, 792
Bsc4I CCNNNNNNNGG 3 cut(s) 22, 448, 594
Bse118I RCCGGY 1 cut(s) 792
Bse1I ACTGG 3 cut(s) 486, 525, 599
BseAI TCCGGA 1 cut(s) 777
BseBI CCWGG 2 cut(s) 22, 130
BseDI CCNNGG 6 cut(s) 21, 128, 129, 273, 610, 750
BseGI GGATG 1 cut(s) 504
BseLI CCNNNNNNNGG 3 cut(s) 22, 448, 594
BseMII CTCAG 1 cut(s) 801
BseNI ACTGG 3 cut(s) 486, 525, 599
BseRI GAGGAG 2 cut(s) 50, 434
BshFI GGCC 1 cut(s) 126
BshNI GGYRCC 1 cut(s) 269
BshTI ACCGGT 1 cut(s) 792
BsiSI CCGG 2 cut(s) 778, 793
BslFI GGGAC 2 cut(s) 485, 700
BslI CCNNNNNNNGG 3 cut(s) 22, 448, 594
BsmFI GGGAC 2 cut(s) 485, 700
BsnI GGCC 1 cut(s) 126
Bsp13I TCCGGA 1 cut(s) 777
Bsp143I GATC 6 cut(s) 38, 118, 308, 374, 547, 754
BspACI CCGC 4 cut(s) 10, 42, 66, 78
BspANI GGCC 1 cut(s) 126
BspCNI CTCAG 1 cut(s) 800
BspEI TCCGGA 1 cut(s) 777
BspLI GGNNCC 4 cut(s) 127, 135, 271, 420
BspPI GGATC 4 cut(s) 33, 126, 555, 762
BspT107I GGYRCC 1 cut(s) 269
BspTI CTTAAG 1 cut(s) 542
BsrFI RCCGGY 1 cut(s) 792
BsrI ACTGG 3 cut(s) 486, 525, 599
BssAI RCCGGY 1 cut(s) 792
BssECI CCNNGG 6 cut(s) 21, 128, 129, 273, 610, 750
BssMI GATC 6 cut(s) 38, 118, 308, 374, 547, 754
BssNI GRCGYC 1 cut(s) 278
BssT1I CCWWGG 2 cut(s) 610, 750
Bst2UI CCWGG 2 cut(s) 22, 130
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 1 cut(s) 59
BstACI GRCGYC 1 cut(s) 278
BstAFI CTTAAG 1 cut(s) 542
BstC8I GCNNGC 6 cut(s) 28, 82, 147, 227, 408, 643
BstDEI CTNAG 2 cut(s) 466, 787
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 1 cut(s) 504
BstHHI GCGC 1 cut(s) 32
BstKTI GATC 6 cut(s) 41, 121, 311, 377, 550, 757
BstMBI GATC 6 cut(s) 38, 118, 308, 374, 547, 754
BstMWI GCNNNNNNNGC 2 cut(s) 403, 692
BstNI CCWGG 2 cut(s) 22, 130
BstNSI RCATGY 1 cut(s) 433
BstSCI CCNGG 2 cut(s) 20, 128
BstV2I GAAGAC 1 cut(s) 40
BstX2I RGATCY 5 cut(s) 118, 308, 374, 547, 754
BstYI RGATCY 5 cut(s) 118, 308, 374, 547, 754
BsuRI GGCC 1 cut(s) 126
BtgI CCRYGG 1 cut(s) 273
BtsCI GGATG 1 cut(s) 504
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 3 cut(s) 518, 592, 687
Cac8I GCNNGC 6 cut(s) 28, 82, 147, 227, 408, 643
CaiI CAGNNNCTG 2 cut(s) 191, 686
CfoI GCGC 1 cut(s) 32
Cfr10I RCCGGY 1 cut(s) 792
Cfr13I GGNCC 2 cut(s) 125, 134
CseI GACGC 1 cut(s) 267
Csp6I GTAC 2 cut(s) 479, 534
CspAI ACCGGT 1 cut(s) 792
CspCI CAANNNNNGTGG 2 cut(s) 496, 531
CviAII CATG 1 cut(s) 430
CviQI GTAC 2 cut(s) 479, 534
DdeI CTNAG 2 cut(s) 466, 787
DpnI GATC 6 cut(s) 40, 120, 310, 376, 549, 756
DpnII GATC 6 cut(s) 38, 118, 308, 374, 547, 754
DraIII CACNNNGTG 2 cut(s) 206, 598
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
EciI GGCGGA 1 cut(s) 31
Eco130I CCWWGG 2 cut(s) 610, 750
Eco47I GGWCC 1 cut(s) 134
Eco57I CTGAAG 1 cut(s) 782
EcoRII CCWGG 2 cut(s) 20, 128
EcoT14I CCWWGG 2 cut(s) 610, 750
ErhI CCWWGG 2 cut(s) 610, 750
FaeI CATG 1 cut(s) 433
FaiI YATR 8 cut(s) 106, 157, 315, 431, 477, 528, 605, 773
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FaqI GGGAC 2 cut(s) 485, 700
FatI CATG 1 cut(s) 429
Fnu4HI GCNGC 1 cut(s) 66
FokI GGATG 1 cut(s) 511
Fsp4HI GCNGC 1 cut(s) 66
FspBI CTAG 1 cut(s) 611
GlaI GCGC 1 cut(s) 31
GluI GCNGC 1 cut(s) 66
HaeIII GGCC 1 cut(s) 126
HapII CCGG 2 cut(s) 778, 793
HgaI GACGC 1 cut(s) 267
HhaI GCGC 1 cut(s) 32
Hin1I GRCGYC 1 cut(s) 278
Hin1II CATG 1 cut(s) 433
Hin6I GCGC 1 cut(s) 30
HinP1I GCGC 1 cut(s) 30
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HinfI GANTC 6 cut(s) 237, 248, 454, 631, 743, 785
HpaI GTTAAC 1 cut(s) 463
HpaII CCGG 2 cut(s) 778, 793
HphI GGTGA 2 cut(s) 67, 463
Hpy166II GTNNAC 2 cut(s) 137, 463
Hpy188I TCNGA 5 cut(s) 38, 556, 737, 762, 790
Hpy188III TCNNGA 3 cut(s) 100, 331, 778
Hpy8I GTNNAC 2 cut(s) 137, 463
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 4 cut(s) 43, 85, 698, 729
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 6 cut(s) 155, 260, 365, 518, 645, 659
HpyF10VI GCNNNNNNNGC 2 cut(s) 403, 692
HpyF3I CTNAG 2 cut(s) 466, 787
HpySE526I ACGT 1 cut(s) 558
Hsp92I GRCGYC 1 cut(s) 278
Hsp92II CATG 1 cut(s) 433
HspAI GCGC 1 cut(s) 30
Kpn2I TCCGGA 1 cut(s) 777
KspAI GTTAAC 1 cut(s) 463
Kzo9I GATC 6 cut(s) 38, 118, 308, 374, 547, 754
LmnI GCTCC 2 cut(s) 415, 737
LweI GCATC 3 cut(s) 492, 494, 717
MaeI CTAG 1 cut(s) 611
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 4 cut(s) 73, 316, 451, 559
MalI GATC 6 cut(s) 40, 120, 310, 376, 549, 756
MboI GATC 6 cut(s) 38, 118, 308, 374, 547, 754
MboII GAAGA 5 cut(s) 40, 46, 306, 318, 749
MfeI CAATTG 1 cut(s) 300
MflI RGATCY 5 cut(s) 118, 308, 374, 547, 754
MluCI AATT 3 cut(s) 300, 334, 366
MlyI GAGTC 3 cut(s) 242, 448, 779
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 9 cut(s) 26, 62, 68, 71, 174, 203, 452, 455, 777
MroI TCCGGA 1 cut(s) 777
MroXI GAANNNNTTC 1 cut(s) 95
MseI TTAA 6 cut(s) 111, 384, 426, 462, 543, 797
MspCI CTTAAG 1 cut(s) 542
MspI CCGG 2 cut(s) 778, 793
MspR9I CCNGG 2 cut(s) 22, 130
MunI CAATTG 1 cut(s) 300
MvaI CCWGG 2 cut(s) 22, 130
MwoI GCNNNNNNNGC 2 cut(s) 403, 692
NdeII GATC 6 cut(s) 38, 118, 308, 374, 547, 754
NlaIII CATG 1 cut(s) 433
NlaIV GGNNCC 4 cut(s) 127, 135, 271, 420
NmuCI GTSAC 3 cut(s) 73, 316, 451
NspI RCATGY 1 cut(s) 433
PasI CCCWGGG 1 cut(s) 129
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 3 cut(s) 237, 631, 743
PflMI CCANNNNNTGG 1 cut(s) 594
PinAI ACCGGT 1 cut(s) 792
PkrI GCNGC 1 cut(s) 67
PleI GAGTC 3 cut(s) 242, 448, 779
PpsI GAGTC 3 cut(s) 242, 448, 779
Psp6I CCWGG 2 cut(s) 20, 128
PspGI CCWGG 2 cut(s) 20, 128
PspN4I GGNNCC 4 cut(s) 127, 135, 271, 420
PspPI GGNCC 2 cut(s) 125, 134
PstNI CAGNNNCTG 2 cut(s) 191, 686
PsuI RGATCY 5 cut(s) 118, 308, 374, 547, 754
RsaI GTAC 2 cut(s) 480, 535
RsaNI GTAC 2 cut(s) 479, 534
SaqAI TTAA 6 cut(s) 111, 384, 426, 462, 543, 797
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 6 cut(s) 38, 118, 308, 374, 547, 754
Sau96I GGNCC 2 cut(s) 125, 134
SchI GAGTC 3 cut(s) 242, 448, 779
ScrFI CCNGG 2 cut(s) 22, 130
SetI ASST 8 cut(s) 18, 190, 214, 271, 355, 444, 561, 616
SfaNI GCATC 3 cut(s) 492, 494, 717
SinI GGWCC 1 cut(s) 134
SmlI CTYRAG 2 cut(s) 329, 542
SmoI CTYRAG 2 cut(s) 329, 542
Sse9I AATT 3 cut(s) 300, 334, 366
SsiI CCGC 4 cut(s) 10, 42, 66, 78
SspMI CTAG 1 cut(s) 611
StyD4I CCNGG 2 cut(s) 20, 128
StyI CCWWGG 2 cut(s) 610, 750
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 1 cut(s) 561
TasI AATT 3 cut(s) 300, 334, 366
TatI WGTACW 1 cut(s) 478
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 3 cut(s) 237, 631, 743
Tru1I TTAA 6 cut(s) 111, 384, 426, 462, 543, 797
Tru9I TTAA 6 cut(s) 111, 384, 426, 462, 543, 797
TscAI CASTG 3 cut(s) 525, 599, 687
TseFI GTSAC 3 cut(s) 73, 316, 451
Tsp45I GTSAC 3 cut(s) 73, 316, 451
TspDTI ATGAA 1 cut(s) 657
TspGWI ACGGA 1 cut(s) 616
TspRI CASTG 3 cut(s) 525, 599, 687
Van91I CCANNNNNTGG 1 cut(s) 594
Vha464I CTTAAG 1 cut(s) 542
VpaK11BI GGWCC 1 cut(s) 134
XceI RCATGY 1 cut(s) 433
XcmI CCANNNNNNNNNTGG 1 cut(s) 517
XmaJI CCTAGG 1 cut(s) 610
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.