Rroxscaffold_7G00176070

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017506
Physical Location & Seq
Forward (+)
15551523 .. 15570347
18825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00176070.1

Sequence Viewer

Length: 1074 bp
ATGTCTTTGTCCCTTGTGGCGGTCGTGTCCGGCATACCAGGATTTGCAGCAAGAAGGGCGATATGGAAAGCGGTGTTTGGTGTCGTGCTGTTGGAGATAGATCACCGAATGATGAGCAAAGGGCCCACAACTTTTCTAGGATGGAGACGATGTGCGATAGCAGCCCGCTCGTCGTTTAGGCCTGATGGGGCCCGCAGTTTCGACGGCGACGACGACCGTCATCTGAGATCTTGGCGTCGTAAACTCCTCTCCTCCCTCACCGCCGTCAAACTCAAGCTCACTTGGACCTCCGCCTTCAGATTCGCCCTCCTCCTCTTCCTCCTCGCCGCCATTGCCACCGCCTGCATTTCCCTCCCAATCGAAAAGAGTTTGAAGGATTTCTTGTTATGGATTAAGCAGGATCTTGGACCTTGGGGTCCACTTGTTTTGGCTGTCGCATACGTTCCTCTCACGGTGCTGGCAGTTCCGGCTTCTGTACTTACTCTTGGTGGAGGTTATCTTTTTGGTTTGCTCGTGGGATTCATTGCAGACTCTATTGGTGCAACATTAGGTGCAACAGCGGCATTCATTCTTGGAAGAACAATTGGAAGATCTTATGTTATTTCCAAACTAAAGCATTATCCAAAGTTTCAAGCTGTTGCTGTTGCCATTCAGAGATCTGGTTTCAAGATAGTTCTTCTGCTGCGGCTTGCTCCTTTCCTTCCATTTAACATGCTGAACTACCTACTCTCTGTGACTCCTGTTCACATAGGGGACTATATGCTGGCAACTTGGTTGGGAATGATGCCCATCACATTTGCACTGGTATATGTTGGTACAACTCTTAAGGATCTTTCAGATGTTACACATGGATGGAATGAAGTTTCAACAACTCGTTGGGTGTTTATAGTACTTGGTTTTGGAATATCCGTGATTCTTCTGATTTGCATTAGTAAAGTTGCAAAGGCTTCTTTGGAAAAAGCACTGGCAGACAATGCTGAAATAGAAGGGATATTCTCGTCTTCAACGCTGCCCATTGTAGCAGGTTCATCCCCGGATCTTCCAAAAACTCTCATCATACAGATAGACTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

357

Amino Acids

38.77

Weight (kDa)

9.87

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 155 - 273 1.3e-24 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03260
fragaria_vesca FvH4_2g23830
malus_domestica MD03G1267800.v1.1
rosa_chinensis RchiOBHm_Chr6g0291411
rosa_laevigata RLG00000012116
rosa_multiflora Rmu_sc0003735.1_g000021
rosa_roxburghii Rroxscaffold_7G00176070
rosa_rugosa Rorug06G0219100
rosa_samantha Rh6AG331400 Rh6BG338300 Rh6CG344600
rosa_wichuraiana Rw6G028770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 414
Acc36I ACCTGC 1 cut(s) 1013
AccBSI CCGCTC 1 cut(s) 168
AclWI GGATC 3 cut(s) 408, 837, 1044
AcuI CTGAAG 1 cut(s) 280
AcyI GRCGYC 1 cut(s) 235
AfaI GTAC 3 cut(s) 477, 817, 891
AfiI CCNNNNNNNGG 1 cut(s) 19
AflII CTTAAG 1 cut(s) 824
AgsI TTSAA 5 cut(s) 373, 632, 667, 867, 1005
AjnI CCWGG 1 cut(s) 37
AluBI AGCT 2 cut(s) 277, 635
AluI AGCT 2 cut(s) 277, 635
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 3 cut(s) 408, 837, 1044
AoxI GGCC 3 cut(s) 122, 179, 189
ApaI GGGCCC 2 cut(s) 126, 193
ApeKI GCWGC 4 cut(s) 47, 161, 682, 1009
Asp700I GAANNNNTTC 1 cut(s) 377
AspS9I GGNCC 7 cut(s) 122, 123, 189, 190, 285, 407, 416
AsuC2I CCSGG 1 cut(s) 1034
AsuHPI GGTGA 2 cut(s) 95, 250
AvaII GGWCC 3 cut(s) 285, 407, 416
BaeGI GKGCMC 2 cut(s) 126, 193
BanII GRGCYC 2 cut(s) 126, 193
BauI CACGAG 1 cut(s) 512
BbsI GAAGAC 1 cut(s) 993
BbvI GCAGC 4 cut(s) 59, 173, 669, 996
BccI CCATC 4 cut(s) 135, 179, 797, 846
BceAI ACGGC 2 cut(s) 220, 248
BcgI CGANNNNNNTGC 2 cut(s) 150, 184
BciT130I CCWGG 1 cut(s) 39
BcnI CCSGG 1 cut(s) 1034
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 1 cut(s) 137
BfrI CTTAAG 1 cut(s) 824
BfuAI ACCTGC 1 cut(s) 1013
BglII AGATCT 3 cut(s) 227, 590, 656
BisI GCNGC 7 cut(s) 48, 162, 327, 561, 683, 686, 1010
BlsI GCNGC 7 cut(s) 49, 163, 328, 562, 684, 687, 1011
BmcAI AGTACT 1 cut(s) 891
Bme1390I CCNGG 2 cut(s) 39, 1034
Bme18I GGWCC 3 cut(s) 285, 407, 416
BmgT120I GGNCC 7 cut(s) 122, 123, 189, 190, 285, 407, 416
BmiI GGNNCC 4 cut(s) 124, 190, 191, 417
BmrFI CCNGG 2 cut(s) 39, 1034
BmsI GCATC 1 cut(s) 774
BoxI GACNNNNGTC 1 cut(s) 216
BpiI GAAGAC 1 cut(s) 993
BpuEI CTTGAG 1 cut(s) 257
BpuMI CCSGG 1 cut(s) 1034
BsaBI GATNNNNATC 1 cut(s) 788
BsaHI GRCGYC 1 cut(s) 235
BsaJI CCNNGG 2 cut(s) 410, 1032
BsaXI ACNNNNNCTCC 2 cut(s) 136, 166
Bsc4I CCNNNNNNNGG 1 cut(s) 19
Bse1I ACTGG 2 cut(s) 807, 969
Bse3DI GCAATG 2 cut(s) 330, 522
Bse8I GATNNNNATC 1 cut(s) 788
BseBI CCWGG 1 cut(s) 39
BseDI CCNNGG 2 cut(s) 410, 1032
BseGI GGATG 3 cut(s) 146, 857, 1028
BseJI GATNNNNATC 1 cut(s) 788
BseLI CCNNNNNNNGG 1 cut(s) 19
BseMI GCAATG 2 cut(s) 330, 522
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 2 cut(s) 807, 969
BseRI GAGGAG 5 cut(s) 236, 241, 299, 302, 311
BseSI GKGCMC 2 cut(s) 126, 193
BseXI GCAGC 4 cut(s) 59, 173, 669, 996
Bsh1285I CGRYCG 2 cut(s) 24, 217
BshFI GGCC 3 cut(s) 124, 181, 191
BsiEI CGRYCG 2 cut(s) 24, 217
BsiSI CCGG 3 cut(s) 30, 467, 1034
BslFI GGGAC 1 cut(s) 767
BslI CCNNNNNNNGG 1 cut(s) 19
BsmAI GTCTC 1 cut(s) 139
BsmBI CGTCTC 1 cut(s) 139
BsmFI GGGAC 1 cut(s) 767
BsmI GAATGC 1 cut(s) 563
BsnI GGCC 3 cut(s) 124, 181, 191
Bsp120I GGGCCC 2 cut(s) 122, 189
Bsp1286I GDGCHC 2 cut(s) 126, 193
Bsp143I GATC 7 cut(s) 100, 227, 400, 590, 656, 829, 1036
BspANI GGCC 3 cut(s) 124, 181, 191
BspCNI CTCAG 1 cut(s) 216
BspLI GGNNCC 4 cut(s) 124, 190, 191, 417
BspMI ACCTGC 1 cut(s) 1013
BspPI GGATC 3 cut(s) 408, 837, 1044
BspTI CTTAAG 1 cut(s) 824
BsrBI CCGCTC 1 cut(s) 168
BsrDI GCAATG 2 cut(s) 330, 522
BsrI ACTGG 2 cut(s) 807, 969
BssECI CCNNGG 2 cut(s) 410, 1032
BssMI GATC 7 cut(s) 100, 227, 400, 590, 656, 829, 1036
BssNI GRCGYC 1 cut(s) 235
BssSI CACGAG 1 cut(s) 512
BssT1I CCWWGG 1 cut(s) 410
Bst2BI CACGAG 1 cut(s) 512
Bst2UI CCWGG 1 cut(s) 39
Bst4CI ACNGT 2 cut(s) 218, 454
Bst6I CTCTTC 1 cut(s) 320
BstACI GRCGYC 1 cut(s) 235
BstAFI CTTAAG 1 cut(s) 824
BstAPI GCANNNNNTGC 1 cut(s) 974
BstC8I GCNNGC 6 cut(s) 166, 193, 343, 459, 690, 765
BstDEI CTNAG 1 cut(s) 224
BstF5I GGATG 3 cut(s) 146, 857, 1028
BstKTI GATC 7 cut(s) 103, 230, 403, 593, 659, 832, 1039
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 7 cut(s) 100, 227, 400, 590, 656, 829, 1036
BstMCI CGRYCG 2 cut(s) 24, 217
BstMWI GCNNNNNNNGC 6 cut(s) 56, 161, 332, 467, 560, 974
BstNI CCWGG 1 cut(s) 39
BstNSI RCATGY 1 cut(s) 715
BstPAI GACNNNNGTC 1 cut(s) 216
BstSCI CCNGG 2 cut(s) 37, 1032
BstSLI GKGCMC 2 cut(s) 126, 193
BstV1I GCAGC 4 cut(s) 59, 173, 669, 996
BstV2I GAAGAC 1 cut(s) 993
BstX2I RGATCY 6 cut(s) 227, 400, 590, 656, 829, 1036
BstYI RGATCY 6 cut(s) 227, 400, 590, 656, 829, 1036
BsuRI GGCC 3 cut(s) 124, 181, 191
BtsCI GGATG 3 cut(s) 146, 857, 1028
BtsIMutI CAGTG 2 cut(s) 800, 962
BveI ACCTGC 1 cut(s) 1013
Cac8I GCNNGC 6 cut(s) 166, 193, 343, 459, 690, 765
Cfr13I GGNCC 7 cut(s) 122, 123, 189, 190, 285, 407, 416
CseI GACGC 1 cut(s) 224
Csp6I GTAC 3 cut(s) 476, 816, 890
CspCI CAANNNNNGTGG 2 cut(s) 408, 443
CviAII CATG 2 cut(s) 712, 848
CviQI GTAC 3 cut(s) 476, 816, 890
DdeI CTNAG 1 cut(s) 224
DpnI GATC 7 cut(s) 102, 229, 402, 592, 658, 831, 1038
DpnII GATC 7 cut(s) 100, 227, 400, 590, 656, 829, 1036
DrdI GACNNNNNNGTC 1 cut(s) 414
DseDI GACNNNNNNGTC 1 cut(s) 414
Eam1104I CTCTTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 320
EciI GGCGGA 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 410
Eco147I AGGCCT 1 cut(s) 181
Eco24I GRGCYC 2 cut(s) 126, 193
Eco47I GGWCC 3 cut(s) 285, 407, 416
Eco57I CTGAAG 1 cut(s) 280
EcoO109I RGGNCCY 2 cut(s) 122, 189
EcoRII CCWGG 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 410
EcoT38I GRGCYC 2 cut(s) 126, 193
ErhI CCWWGG 1 cut(s) 410
Esp3I CGTCTC 1 cut(s) 139
FaeI CATG 2 cut(s) 715, 851
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FaqI GGGAC 1 cut(s) 767
FatI CATG 2 cut(s) 711, 847
FauI CCCGC 2 cut(s) 173, 200
Fnu4HI GCNGC 7 cut(s) 48, 162, 327, 561, 683, 686, 1010
FokI GGATG 3 cut(s) 153, 864, 1015
FriOI GRGCYC 2 cut(s) 126, 193
Fsp4HI GCNGC 7 cut(s) 48, 162, 327, 561, 683, 686, 1010
FspBI CTAG 1 cut(s) 137
GluI GCNGC 7 cut(s) 48, 162, 327, 561, 683, 686, 1010
HaeIII GGCC 3 cut(s) 124, 181, 191
HapII CCGG 3 cut(s) 30, 467, 1034
HgaI GACGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 235
Hin1II CATG 2 cut(s) 715, 851
HinfI GANTC 6 cut(s) 300, 519, 530, 736, 913, 1067
HpaII CCGG 3 cut(s) 30, 467, 1034
HphI GGTGA 2 cut(s) 95, 250
Hpy166II GTNNAC 3 cut(s) 242, 419, 745
Hpy188I TCNGA 5 cut(s) 225, 299, 654, 838, 921
Hpy188III TCNNGA 1 cut(s) 667
Hpy8I GTNNAC 3 cut(s) 242, 419, 745
Hpy99I CGWCG 5 cut(s) 175, 206, 212, 215, 240
HpyAV CCTTC 5 cut(s) 48, 304, 367, 710, 980
HpyCH4III ACNGT 2 cut(s) 218, 454
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 8 cut(s) 47, 345, 527, 542, 554, 800, 927, 941
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 161, 332, 467, 560, 974
HpyF3I CTNAG 1 cut(s) 224
HpySE526I ACGT 1 cut(s) 441
Hsp92I GRCGYC 1 cut(s) 235
Hsp92II CATG 2 cut(s) 715, 851
Kzo9I GATC 7 cut(s) 100, 227, 400, 590, 656, 829, 1036
LmnI GCTCC 1 cut(s) 697
Lsp1109I GCAGC 4 cut(s) 59, 173, 669, 996
LweI GCATC 1 cut(s) 774
MaeI CTAG 1 cut(s) 137
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 2 cut(s) 733, 841
MalI GATC 7 cut(s) 102, 229, 402, 592, 658, 831, 1038
MbiI CCGCTC 1 cut(s) 168
MboI GATC 7 cut(s) 100, 227, 400, 590, 656, 829, 1036
MboII GAAGA 7 cut(s) 307, 588, 600, 668, 908, 993, 1031
MfeI CAATTG 1 cut(s) 582
MflI RGATCY 6 cut(s) 227, 400, 590, 656, 829, 1036
MhlI GDGCHC 2 cut(s) 126, 193
MluCI AATT 1 cut(s) 582
MlyI GAGTC 3 cut(s) 524, 730, 1061
MmeI TCCRAC 1 cut(s) 72
MroXI GAANNNNTTC 1 cut(s) 377
MseI TTAA 3 cut(s) 393, 708, 825
MslI CAYNNNNRTG 1 cut(s) 850
MspA1I CMGCKG 1 cut(s) 560
MspCI CTTAAG 1 cut(s) 824
MspI CCGG 3 cut(s) 30, 467, 1034
MspR9I CCNGG 2 cut(s) 39, 1034
MunI CAATTG 1 cut(s) 582
Mva1269I GAATGC 1 cut(s) 563
MvaI CCWGG 1 cut(s) 39
MwoI GCNNNNNNNGC 6 cut(s) 56, 161, 332, 467, 560, 974
NciI CCSGG 1 cut(s) 1034
NdeII GATC 7 cut(s) 100, 227, 400, 590, 656, 829, 1036
NlaIII CATG 2 cut(s) 715, 851
NlaIV GGNNCC 4 cut(s) 124, 190, 191, 417
NmuCI GTSAC 1 cut(s) 733
NspI RCATGY 1 cut(s) 715
PceI AGGCCT 1 cut(s) 181
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 210
PctI GAATGC 1 cut(s) 563
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 3 cut(s) 300, 519, 913
PkrI GCNGC 7 cut(s) 49, 163, 328, 562, 684, 687, 1011
PleI GAGTC 3 cut(s) 524, 730, 1061
PpsI GAGTC 3 cut(s) 524, 730, 1061
PshAI GACNNNNGTC 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 37
PspGI CCWGG 1 cut(s) 37
PspN4I GGNNCC 4 cut(s) 124, 190, 191, 417
PspOMI GGGCCC 2 cut(s) 122, 189
PspPI GGNCC 7 cut(s) 122, 123, 189, 190, 285, 407, 416
PsuI RGATCY 6 cut(s) 227, 400, 590, 656, 829, 1036
RsaI GTAC 3 cut(s) 477, 817, 891
RsaNI GTAC 3 cut(s) 476, 816, 890
RseI CAYNNNNRTG 1 cut(s) 850
SaqAI TTAA 3 cut(s) 393, 708, 825
SatI GCNGC 7 cut(s) 48, 162, 327, 561, 683, 686, 1010
Sau3AI GATC 7 cut(s) 100, 227, 400, 590, 656, 829, 1036
Sau96I GGNCC 7 cut(s) 122, 123, 189, 190, 285, 407, 416
ScaI AGTACT 1 cut(s) 891
SchI GAGTC 3 cut(s) 524, 730, 1061
ScrFI CCNGG 2 cut(s) 39, 1034
SduI GDGCHC 2 cut(s) 126, 193
SetI ASST 9 cut(s) 279, 290, 412, 444, 496, 553, 637, 726, 1027
SfaNI GCATC 1 cut(s) 774
SinI GGWCC 3 cut(s) 285, 407, 416
SmiMI CAYNNNNRTG 1 cut(s) 850
SmlI CTYRAG 2 cut(s) 272, 824
SmoI CTYRAG 2 cut(s) 272, 824
Sse9I AATT 1 cut(s) 582
SseBI AGGCCT 1 cut(s) 181
SspMI CTAG 1 cut(s) 137
StuI AGGCCT 1 cut(s) 181
StyD4I CCNGG 2 cut(s) 37, 1032
StyI CCWWGG 1 cut(s) 410
TaaI ACNGT 2 cut(s) 218, 454
TaiI ACGT 1 cut(s) 444
TaqI TCGA 2 cut(s) 201, 360
TasI AATT 1 cut(s) 582
TatI WGTACW 2 cut(s) 475, 889
TauI GCSGC 3 cut(s) 329, 563, 688
TfiI GAWTC 3 cut(s) 300, 519, 913
Tru1I TTAA 3 cut(s) 393, 708, 825
Tru9I TTAA 3 cut(s) 393, 708, 825
TscAI CASTG 2 cut(s) 807, 969
TseFI GTSAC 1 cut(s) 733
TseI GCWGC 4 cut(s) 47, 161, 682, 1009
Tsp45I GTSAC 1 cut(s) 733
TspDTI ATGAA 4 cut(s) 511, 556, 873, 1017
TspGWI ACGGA 1 cut(s) 898
TspRI CASTG 2 cut(s) 807, 969
Vha464I CTTAAG 1 cut(s) 824
VpaK11BI GGWCC 3 cut(s) 285, 407, 416
XceI RCATGY 1 cut(s) 715
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 1 cut(s) 137
ZrmI AGTACT 1 cut(s) 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.