FvH4_2g24060

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014665
Physical Location & Seq
Reverse (-)
19650339 .. 19652055
1717 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24060.t2

Sequence Viewer

Length: 1122 bp
ATGGCAATGAAAGTAGTCTTTAACCTTCATTTCCTAGCCTTTGTTTCTGTACTGTTACCTATAACTTGTATTCCCATCAATCTCACTTATCCGGCAGTTTTTAACTTCGGTGATTCGAATTCAGACACCGGAGGCATCCAAGCAGGAGTCGGTTTTCCCATTAAACGCTTCTGCAATGGCTATTTGATCATTGACTATTTTGTGACTGCAATGAACCTATCCTATTTGAATCCTTACTTGGATTCAGTTGGTGCACCAAGCTTCCAAACAGGATGTAATTTCGCAACCGGAGGGGCTACCATTCTTCCAGCCAAAGCATCTGCAACAAATCCATTTTCCTATGGAATTCAGGTTGCTCAATTCGTACGATTCAAGGCTCGAGTTCTTGAGTTACTGGCTAAGAAAGACAAGAAGCTTCAACCAGTCCTCCCATATGAAGATTATTTCAAGCAAGGCCTATACACTTTTGATGTGGGACAGAATGATCTTGATGGTGCATTTTATTCCAAATCAGAAGACCAAGTTGTTGCTTCAATTCCAGCTATTCTGACAGAATTGGAGACCGGATTTGAGAAACTATATAACCAGGGGGCTAGGAATTTTTGGATTCATAACACAGGTCCACTAGGATGCCTACCAAGAATTATAGCAACATTTGGGAAAGATCCATCATATCTGAACCAGGTTGGATGTGTTGCTTCACATAACCGTGCTGCTACTGCTTTCAATGAACAGCTACATGATCTTTGCTTGAAATTCAAGAAGAATTTTCCTGAAGCTAATGTTACTTATGTTGACATCTTCAAGATAAAGTCGGACCTCATCACAAACTATGCTCAATATGGTTTCAAACAAGGCATAGCTGCTTGCTGTGGGTACGGTGGTCCGCCTTTAAATTTTGATAACCGGATAGCTTGTGGCCAAACCAAGAATCTGAATGGGACTGTGGTCACTGCTACACCGTGCAGTAATCCAGCCGAGTATGTGAATTGGGATGGTAATCACTATACAGAAGCTGCAAATCAATACGTATCCTCGCAGATATTAATAGGAAACTACACAGACTACTTCAACTCTTCCATTGAAGGCAAACTTTCTTATGGGAATCCAAACGTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

374

Amino Acids

41.0

Weight (kDa)

6.22

Isoelectric Point (pI)

21.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 353 2.4e-45 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 887
AclI AACGTT 1 cut(s) 1113
AclWI GGATC 1 cut(s) 659
AcoI YGGCCR 1 cut(s) 919
AcsI RAATTY 6 cut(s) 118, 345, 598, 755, 766, 895
AcuI CTGAAG 1 cut(s) 795
AfaI GTAC 3 cut(s) 51, 366, 878
AjnI CCWGG 2 cut(s) 585, 681
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 8 cut(s) 261, 415, 542, 736, 779, 863, 914, 1016
AluI AGCT 8 cut(s) 261, 415, 542, 736, 779, 863, 914, 1016
Alw21I GWGCWC 1 cut(s) 256
Alw26I GTCTC 1 cut(s) 554
Alw44I GTGCAC 1 cut(s) 252
AlwI GGATC 1 cut(s) 659
AlwNI CAGNNNCTG 1 cut(s) 1016
Ama87I CYCGRG 1 cut(s) 378
AoxI GGCC 2 cut(s) 454, 919
ApaLI GTGCAC 1 cut(s) 252
ApeKI GCWGC 3 cut(s) 713, 863, 1016
ApoI RAATTY 6 cut(s) 118, 345, 598, 755, 766, 895
AseI ATTAAT 1 cut(s) 1046
AspS9I GGNCC 3 cut(s) 620, 817, 884
AsuHPI GGTGA 1 cut(s) 122
AsuII TTCGAA 1 cut(s) 116
AvaI CYCGRG 1 cut(s) 378
AvaII GGWCC 3 cut(s) 620, 817, 884
BaeGI GKGCMC 1 cut(s) 256
BaeI ACNNNNGTAYC 2 cut(s) 868, 901
BalI TGGCCA 1 cut(s) 921
BbsI GAAGAC 1 cut(s) 522
Bbv12I GWGCWC 1 cut(s) 256
BbvI GCAGC 3 cut(s) 700, 850, 1003
BccI CCATC 4 cut(s) 83, 485, 676, 989
BciT130I CCWGG 2 cut(s) 587, 683
BciVI GTATCC 1 cut(s) 1042
BclI TGATCA 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 554
BfaI CTAG 3 cut(s) 35, 594, 626
BfuI GTATCC 1 cut(s) 1042
BisI GCNGC 3 cut(s) 714, 864, 1017
BlsI GCNGC 3 cut(s) 715, 865, 1018
Bme1390I CCNGG 2 cut(s) 587, 683
Bme18I GGWCC 3 cut(s) 620, 817, 884
BmeT110I CYCGRG 1 cut(s) 378
BmgT120I GGNCC 3 cut(s) 620, 817, 884
BmrFI CCNGG 2 cut(s) 587, 683
BmsI GCATC 3 cut(s) 144, 326, 620
BoxI GACNNNNGTC 1 cut(s) 947
BpiI GAAGAC 1 cut(s) 522
Bpu14I TTCGAA 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 407
BsaAI YACGTR 1 cut(s) 1030
BsaBI GATNNNNATC 1 cut(s) 999
BsaI GGTCTC 1 cut(s) 554
BsaJI CCNNGG 1 cut(s) 586
BsaWI WCCGGW 4 cut(s) 128, 287, 563, 906
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bse1I ACTGG 2 cut(s) 399, 422
Bse3DI GCAATG 3 cut(s) 12, 181, 216
Bse8I GATNNNNATC 1 cut(s) 999
BseBI CCWGG 2 cut(s) 587, 683
BseDI CCNNGG 1 cut(s) 586
BseGI GGATG 5 cut(s) 135, 278, 635, 695, 1000
BseJI GATNNNNATC 1 cut(s) 999
BseMI GCAATG 3 cut(s) 12, 181, 216
BseNI ACTGG 2 cut(s) 399, 422
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 3 cut(s) 700, 850, 1003
BsgI GTGCAG 1 cut(s) 985
BshFI GGCC 2 cut(s) 456, 921
BsiHKAI GWGCWC 1 cut(s) 256
BsiHKCI CYCGRG 1 cut(s) 378
BsiSI CCGG 5 cut(s) 92, 129, 288, 564, 907
BsiWI CGTACG 1 cut(s) 364
BslFI GGGAC 2 cut(s) 489, 955
BsmAI GTCTC 1 cut(s) 554
BsmFI GGGAC 2 cut(s) 489, 955
BsnI GGCC 2 cut(s) 456, 921
Bso31I GGTCTC 1 cut(s) 554
BsoBI CYCGRG 1 cut(s) 378
Bsp119I TTCGAA 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 256
Bsp143I GATC 4 cut(s) 186, 484, 664, 742
BspACI CCGC 1 cut(s) 887
BspANI GGCC 2 cut(s) 456, 921
BspPI GGATC 1 cut(s) 659
BspT104I TTCGAA 1 cut(s) 116
BspTNI GGTCTC 1 cut(s) 554
BsrDI GCAATG 3 cut(s) 12, 181, 216
BsrI ACTGG 2 cut(s) 399, 422
BssECI CCNNGG 1 cut(s) 586
BssMI GATC 4 cut(s) 186, 484, 664, 742
Bst2UI CCWGG 2 cut(s) 587, 683
Bst4CI ACNGT 5 cut(s) 54, 710, 881, 946, 963
Bst6I CTCTTC 1 cut(s) 1081
BstBAI YACGTR 1 cut(s) 1030
BstBI TTCGAA 1 cut(s) 116
BstC8I GCNNGC 1 cut(s) 868
BstDEI CTNAG 1 cut(s) 399
BstF5I GGATG 5 cut(s) 135, 278, 635, 695, 1000
BstKTI GATC 4 cut(s) 189, 487, 667, 745
BstMAI GTCTC 1 cut(s) 554
BstMBI GATC 4 cut(s) 186, 484, 664, 742
BstMWI GCNNNNNNNGC 1 cut(s) 719
BstNI CCWGG 2 cut(s) 587, 683
BstPAI GACNNNNGTC 1 cut(s) 947
BstSCI CCNGG 2 cut(s) 585, 681
BstSLI GKGCMC 1 cut(s) 256
BstSNI TACGTA 1 cut(s) 1030
BstV1I GCAGC 3 cut(s) 700, 850, 1003
BstV2I GAAGAC 1 cut(s) 522
BstX2I RGATCY 1 cut(s) 664
BstYI RGATCY 1 cut(s) 664
BsuI GTATCC 1 cut(s) 1042
BsuRI GGCC 2 cut(s) 456, 921
BtsCI GGATG 5 cut(s) 135, 278, 635, 695, 1000
BtsI GCAGTG 1 cut(s) 951
BtsIMutI CAGTG 1 cut(s) 951
Cac8I GCNNGC 1 cut(s) 868
CaiI CAGNNNCTG 1 cut(s) 1016
Cfr13I GGNCC 3 cut(s) 620, 817, 884
CsiI ACCWGGT 1 cut(s) 681
Csp6I GTAC 3 cut(s) 50, 365, 877
CviAII CATG 1 cut(s) 740
CviQI GTAC 3 cut(s) 50, 365, 877
DdeI CTNAG 1 cut(s) 399
DpnI GATC 4 cut(s) 188, 486, 666, 744
DpnII GATC 4 cut(s) 186, 484, 664, 742
DraI TTTAAA 1 cut(s) 894
EaeI YGGCCR 1 cut(s) 919
Eam1104I CTCTTC 1 cut(s) 1081
EarI CTCTTC 1 cut(s) 1081
EciI GGCGGA 1 cut(s) 876
Eco105I TACGTA 1 cut(s) 1030
Eco147I AGGCCT 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 554
Eco47I GGWCC 3 cut(s) 620, 817, 884
Eco57I CTGAAG 1 cut(s) 795
Eco88I CYCGRG 1 cut(s) 378
EcoRI GAATTC 2 cut(s) 118, 345
EcoRII CCWGG 2 cut(s) 585, 681
FaeI CATG 1 cut(s) 743
FalI AAGNNNNNCTT 2 cut(s) 1077, 1109
FaqI GGGAC 2 cut(s) 489, 955
FatI CATG 1 cut(s) 739
FauNDI CATATG 1 cut(s) 433
FbaI TGATCA 1 cut(s) 186
Fnu4HI GCNGC 3 cut(s) 714, 864, 1017
FokI GGATG 5 cut(s) 122, 285, 642, 702, 1007
Fsp4HI GCNGC 3 cut(s) 714, 864, 1017
FspBI CTAG 3 cut(s) 35, 594, 626
GluI GCNGC 3 cut(s) 714, 864, 1017
HaeIII GGCC 2 cut(s) 456, 921
HapII CCGG 5 cut(s) 92, 129, 288, 564, 907
Hin1II CATG 1 cut(s) 743
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HindIII AAGCTT 2 cut(s) 259, 413
HinfI GANTC 8 cut(s) 113, 147, 229, 242, 369, 607, 931, 1105
HpaII CCGG 5 cut(s) 92, 129, 288, 564, 907
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 3 cut(s) 254, 623, 796
Hpy188I TCNGA 6 cut(s) 124, 514, 549, 678, 817, 936
Hpy188III TCNNGA 5 cut(s) 386, 488, 760, 773, 805
Hpy8I GTNNAC 3 cut(s) 254, 623, 796
HpyAV CCTTC 2 cut(s) 35, 1079
HpyCH4III ACNGT 5 cut(s) 54, 710, 881, 946, 963
HpyCH4IV ACGT 2 cut(s) 1029, 1113
HpyCH4V TGCA 7 cut(s) 174, 209, 254, 323, 497, 966, 1019
HpyF10VI GCNNNNNNNGC 1 cut(s) 719
HpyF3I CTNAG 1 cut(s) 399
HpySE526I ACGT 2 cut(s) 1029, 1113
Hsp92II CATG 1 cut(s) 743
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 4 cut(s) 186, 484, 664, 742
Lsp1109I GCAGC 3 cut(s) 700, 850, 1003
LweI GCATC 3 cut(s) 144, 326, 620
MabI ACCWGGT 1 cut(s) 681
MaeI CTAG 3 cut(s) 35, 594, 626
MaeII ACGT 2 cut(s) 1029, 1113
MaeIII GTNAC 5 cut(s) 54, 202, 390, 784, 949
MalI GATC 4 cut(s) 188, 486, 666, 744
MboI GATC 4 cut(s) 186, 484, 664, 742
MboII GAAGA 6 cut(s) 296, 449, 527, 775, 793, 1068
MflI RGATCY 1 cut(s) 664
MhlI GDGCHC 1 cut(s) 256
MlsI TGGCCA 1 cut(s) 921
MluNI TGGCCA 1 cut(s) 921
MlyI GAGTC 1 cut(s) 156
MmeI TCCRAC 2 cut(s) 667, 795
MnlI CCTC 5 cut(s) 125, 284, 437, 830, 1045
Mox20I TGGCCA 1 cut(s) 921
MscI TGGCCA 1 cut(s) 921
MseI TTAA 5 cut(s) 21, 102, 162, 893, 1046
MslI CAYNNNNRTG 2 cut(s) 628, 708
Msp20I TGGCCA 1 cut(s) 921
MspI CCGG 5 cut(s) 92, 129, 288, 564, 907
MspR9I CCNGG 2 cut(s) 587, 683
MvaI CCWGG 2 cut(s) 587, 683
MwoI GCNNNNNNNGC 1 cut(s) 719
NdeI CATATG 1 cut(s) 433
NdeII GATC 4 cut(s) 186, 484, 664, 742
NlaIII CATG 1 cut(s) 743
NmeAIII GCCGAG 1 cut(s) 1003
NmuCI GTSAC 2 cut(s) 202, 949
NspV TTCGAA 1 cut(s) 116
PaeR7I CTCGAG 1 cut(s) 378
PceI AGGCCT 1 cut(s) 456
PfeI GAWTC 7 cut(s) 113, 229, 242, 369, 607, 931, 1105
Pfl23II CGTACG 1 cut(s) 364
PkrI GCNGC 3 cut(s) 715, 865, 1018
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
Ppu21I YACGTR 1 cut(s) 1030
PshAI GACNNNNGTC 1 cut(s) 947
PshBI ATTAAT 1 cut(s) 1046
Psp1406I AACGTT 1 cut(s) 1113
Psp6I CCWGG 2 cut(s) 585, 681
PspGI CCWGG 2 cut(s) 585, 681
PspLI CGTACG 1 cut(s) 364
PspPI GGNCC 3 cut(s) 620, 817, 884
PspXI VCTCGAGB 1 cut(s) 378
PstNI CAGNNNCTG 1 cut(s) 1016
PsuI RGATCY 1 cut(s) 664
RsaI GTAC 3 cut(s) 51, 366, 878
RsaNI GTAC 3 cut(s) 50, 365, 877
RseI CAYNNNNRTG 2 cut(s) 628, 708
SaqAI TTAA 5 cut(s) 21, 102, 162, 893, 1046
SatI GCNGC 3 cut(s) 714, 864, 1017
Sau3AI GATC 4 cut(s) 186, 484, 664, 742
Sau96I GGNCC 3 cut(s) 620, 817, 884
SchI GAGTC 1 cut(s) 156
ScrFI CCNGG 2 cut(s) 587, 683
SduI GDGCHC 1 cut(s) 256
SexAI ACCWGGT 1 cut(s) 681
SfaNI GCATC 3 cut(s) 144, 326, 620
Sfr274I CTCGAG 1 cut(s) 378
SfuI TTCGAA 1 cut(s) 116
SinI GGWCC 3 cut(s) 620, 817, 884
SlaI CTCGAG 1 cut(s) 378
SmiMI CAYNNNNRTG 2 cut(s) 628, 708
SmlI CTYRAG 2 cut(s) 378, 386
SmoI CTYRAG 2 cut(s) 378, 386
SnaBI TACGTA 1 cut(s) 1030
SseBI AGGCCT 1 cut(s) 456
SsiI CCGC 1 cut(s) 887
SspMI CTAG 3 cut(s) 35, 594, 626
StuI AGGCCT 1 cut(s) 456
StyD4I CCNGG 2 cut(s) 585, 681
TaaI ACNGT 5 cut(s) 54, 710, 881, 946, 963
TaiI ACGT 2 cut(s) 1032, 1116
TaqI TCGA 2 cut(s) 116, 379
TatI WGTACW 1 cut(s) 49
TfiI GAWTC 7 cut(s) 113, 229, 242, 369, 607, 931, 1105
Tru1I TTAA 5 cut(s) 21, 102, 162, 893, 1046
Tru9I TTAA 5 cut(s) 21, 102, 162, 893, 1046
TscAI CASTG 1 cut(s) 958
TseFI GTSAC 2 cut(s) 202, 949
TseI GCWGC 3 cut(s) 713, 863, 1016
Tsp45I GTSAC 2 cut(s) 202, 949
TspDTI ATGAA 6 cut(s) 17, 23, 227, 450, 599, 744
TspRI CASTG 1 cut(s) 958
VneI GTGCAC 1 cut(s) 252
VpaK11BI GGWCC 3 cut(s) 620, 817, 884
VspI ATTAAT 1 cut(s) 1046
XapI RAATTY 6 cut(s) 118, 345, 598, 755, 766, 895
XhoI CTCGAG 1 cut(s) 378
XspI CTAG 3 cut(s) 35, 594, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.