Rh6DG334900

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0014665
Physical Location & Seq
Reverse (-)
54000797 .. 54002295
1499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG334900.1

Sequence Viewer

Length: 1122 bp
ATGGCTCTGAAAGTAGTCTCCATCCTTCATTTCCTACCCTTTGTGTCTGCACTGTTACCTATAACTAGTATCCCCATCAATCTCACTTATCCGGCAGTTTTTAACTTCGGTGATTCGAATTCAGACACCGGAGGCCTTCAAGCAGGAGTCGGTTTTCCCATTAAACGCTTTTGCAATGGCTATTTGATCATAGACTATTTTGTGACTGCAATGGACCTACCCTATCTGAATCCTTACTTGGATTCAGTTGGTGCACCAAGCTTCCAAACAGGATGTAATTTTGCAACCGGGGGAGCTACCATTCTTCCAGCCAAAGCATCTTCAACAAATCCATTTTCCTTCGGAGTTCAGGTTGCTCAATTTGTCCGATTCAAGGCTCGAGTTCTTGAGCTACTGGCTGAGAAAGACAAGAAGCTTCAACCAGTCCTCCCATATGAAGATTATTTCAAGCAAAGCCTTTACACTTTTGATGTGGGACAGAATGATCTTGATGGTGCATTTTCTTCCAAATCAGAAGACCAAGTTGTTGCTTCAATTCCAGCTATTCTAACAGAACTCGAGACTGGATTTGAGAAACTATACAATCAGGGGGCTAGGAATTTTTGGATCCATAACACAGGTCCACTAGGATGCCTACCAAGAATTATAGCAACATTTGGGAAAGATCCATCAAATTTGAACCAGGTTGGATGTGTTGCTTCACATAACCGTGCTGCTACTGCTTTCAATGAACAACTACATGATCTTTGCTTGAAGTTTAAGAAAAACTTCCCTGAAGCTAATGTTACTTATGTTGACATCTTCACCATAAAGTTGGACCTCATCACAAACTATGCTCAATATGGTTTCAAACAAGGCATAGCAGCTTGCTGTGGGTATGGTGGTCCGCCATTAAATTTTGATAACCGGATAGCTTGTGGCCAAACCAAGAATCTAAATGGGACTGTGGTCACTGCAACACCATGCAGTAATCCAGCTGAGTATGTGAATTGGGATGGTAATCATTATACAGAAGCTGCAAATCAATACGTGTCATCGCAGATATTAACAGGAAACTATTCAGAAGACTTGAACTCTTCGATTGGAAGCAAAATTTCTTATGGGAATCCAAACCTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

373

Amino Acids

40.68

Weight (kDa)

5.43

Isoelectric Point (pI)

26.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 347 1.7e-47 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 887
AclWI GGATC 3 cut(s) 601, 614, 659
AcoI YGGCCR 1 cut(s) 919
AcsI RAATTY 5 cut(s) 118, 598, 673, 895, 1092
AcuI CTGAAG 1 cut(s) 795
AfiI CCNNNNNNNGG 1 cut(s) 373
AflIII ACRYGT 1 cut(s) 1029
AhlI ACTAGT 1 cut(s) 65
AjnI CCWGG 1 cut(s) 681
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
Alw21I GWGCWC 1 cut(s) 256
Alw26I GTCTC 2 cut(s) 22, 554
Alw44I GTGCAC 1 cut(s) 252
AlwI GGATC 3 cut(s) 601, 614, 659
AlwNI CAGNNNCTG 1 cut(s) 1016
Ama87I CYCGRG 2 cut(s) 378, 557
AoxI GGCC 2 cut(s) 133, 919
ApaLI GTGCAC 1 cut(s) 252
ApeKI GCWGC 3 cut(s) 713, 863, 1016
ApoI RAATTY 5 cut(s) 118, 598, 673, 895, 1092
Asp700I GAANNNNTTC 2 cut(s) 767, 1057
AspS9I GGNCC 4 cut(s) 214, 620, 817, 884
AsuC2I CCSGG 1 cut(s) 289
AsuHPI GGTGA 2 cut(s) 122, 796
AsuII TTCGAA 1 cut(s) 116
AvaI CYCGRG 2 cut(s) 378, 557
AvaII GGWCC 4 cut(s) 214, 620, 817, 884
BaeGI GKGCMC 1 cut(s) 256
BalI TGGCCA 1 cut(s) 921
BamHI GGATCC 1 cut(s) 606
BbsI GAAGAC 2 cut(s) 522, 1071
Bbv12I GWGCWC 1 cut(s) 256
BbvI GCAGC 3 cut(s) 700, 875, 1003
BccI CCATC 5 cut(s) 29, 83, 485, 676, 989
BciT130I CCWGG 1 cut(s) 683
BciVI GTATCC 1 cut(s) 80
BclI TGATCA 1 cut(s) 186
BcnI CCSGG 1 cut(s) 289
BcoDI GTCTC 2 cut(s) 22, 554
BcuI ACTAGT 1 cut(s) 65
BfaI CTAG 3 cut(s) 66, 594, 626
BfuI GTATCC 1 cut(s) 80
BisI GCNGC 3 cut(s) 714, 864, 1017
BlsI GCNGC 3 cut(s) 715, 865, 1018
Bme1390I CCNGG 2 cut(s) 289, 683
Bme18I GGWCC 4 cut(s) 214, 620, 817, 884
BmeT110I CYCGRG 2 cut(s) 378, 557
BmgT120I GGNCC 4 cut(s) 214, 620, 817, 884
BmiI GGNNCC 1 cut(s) 608
BmrFI CCNGG 2 cut(s) 289, 683
BmsI GCATC 2 cut(s) 326, 620
BoxI GACNNNNGTC 1 cut(s) 947
BpiI GAAGAC 2 cut(s) 522, 1071
Bpu14I TTCGAA 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 1 cut(s) 289
BsaAI YACGTR 1 cut(s) 1030
BsaBI GATNNNNATC 1 cut(s) 999
BsaJI CCNNGG 1 cut(s) 288
BsaWI WCCGGW 2 cut(s) 128, 906
BsaXI ACNNNNNCTCC 4 cut(s) 336, 366, 411, 441
Bsc4I CCNNNNNNNGG 1 cut(s) 373
Bse1I ACTGG 3 cut(s) 399, 422, 568
Bse3DI GCAATG 2 cut(s) 181, 216
Bse8I GATNNNNATC 1 cut(s) 999
BseBI CCWGG 1 cut(s) 683
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 5 cut(s) 21, 278, 635, 695, 1000
BseJI GATNNNNATC 1 cut(s) 999
BseLI CCNNNNNNNGG 1 cut(s) 373
BseMI GCAATG 2 cut(s) 181, 216
BseMII CTCAG 2 cut(s) 390, 969
BseNI ACTGG 3 cut(s) 399, 422, 568
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 3 cut(s) 700, 875, 1003
BsgI GTGCAG 1 cut(s) 33
BshFI GGCC 2 cut(s) 135, 921
BsiHKAI GWGCWC 1 cut(s) 256
BsiHKCI CYCGRG 2 cut(s) 378, 557
BsiSI CCGG 4 cut(s) 92, 129, 288, 907
BslFI GGGAC 2 cut(s) 489, 955
BslI CCNNNNNNNGG 1 cut(s) 373
BsmAI GTCTC 2 cut(s) 22, 554
BsmFI GGGAC 2 cut(s) 489, 955
BsnI GGCC 2 cut(s) 135, 921
BsoBI CYCGRG 2 cut(s) 378, 557
Bsp119I TTCGAA 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 256
Bsp143I GATC 5 cut(s) 186, 484, 606, 664, 742
BspACI CCGC 1 cut(s) 887
BspANI GGCC 2 cut(s) 135, 921
BspCNI CTCAG 2 cut(s) 391, 970
BspLI GGNNCC 1 cut(s) 608
BspPI GGATC 3 cut(s) 601, 614, 659
BspT104I TTCGAA 1 cut(s) 116
BsrDI GCAATG 2 cut(s) 181, 216
BsrI ACTGG 3 cut(s) 399, 422, 568
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 5 cut(s) 186, 484, 606, 664, 742
Bst2UI CCWGG 1 cut(s) 683
Bst4CI ACNGT 3 cut(s) 54, 710, 946
Bst6I CTCTTC 1 cut(s) 1081
BstBAI YACGTR 1 cut(s) 1030
BstBI TTCGAA 1 cut(s) 116
BstC8I GCNNGC 1 cut(s) 868
BstDEI CTNAG 2 cut(s) 399, 978
BstF5I GGATG 5 cut(s) 21, 278, 635, 695, 1000
BstKTI GATC 5 cut(s) 189, 487, 609, 667, 745
BstMAI GTCTC 2 cut(s) 22, 554
BstMBI GATC 5 cut(s) 186, 484, 606, 664, 742
BstMWI GCNNNNNNNGC 1 cut(s) 719
BstNI CCWGG 1 cut(s) 683
BstPAI GACNNNNGTC 1 cut(s) 947
BstSCI CCNGG 2 cut(s) 287, 681
BstSLI GKGCMC 1 cut(s) 256
BstV1I GCAGC 3 cut(s) 700, 875, 1003
BstV2I GAAGAC 2 cut(s) 522, 1071
BstX2I RGATCY 2 cut(s) 606, 664
BstXI CCANNNNNNTGG 1 cut(s) 814
BstYI RGATCY 2 cut(s) 606, 664
BsuI GTATCC 1 cut(s) 80
BsuRI GGCC 2 cut(s) 135, 921
BtgZI GCGATG 1 cut(s) 1020
BtsCI GGATG 5 cut(s) 21, 278, 635, 695, 1000
BtsI GCAGTG 1 cut(s) 951
BtsIMutI CAGTG 2 cut(s) 50, 951
Cac8I GCNNGC 1 cut(s) 868
CaiI CAGNNNCTG 1 cut(s) 1016
Cfr13I GGNCC 4 cut(s) 214, 620, 817, 884
CsiI ACCWGGT 1 cut(s) 681
CviAII CATG 2 cut(s) 740, 963
DdeI CTNAG 2 cut(s) 399, 978
DpnI GATC 5 cut(s) 188, 486, 608, 666, 744
DpnII GATC 5 cut(s) 186, 484, 606, 664, 742
EaeI YGGCCR 1 cut(s) 919
Eam1104I CTCTTC 1 cut(s) 1081
EarI CTCTTC 1 cut(s) 1081
EciI GGCGGA 1 cut(s) 876
Eco147I AGGCCT 1 cut(s) 135
Eco47I GGWCC 4 cut(s) 214, 620, 817, 884
Eco57I CTGAAG 1 cut(s) 795
Eco88I CYCGRG 2 cut(s) 378, 557
EcoRI GAATTC 1 cut(s) 118
EcoRII CCWGG 1 cut(s) 681
FaeI CATG 2 cut(s) 743, 966
FalI AAGNNNNNCTT 2 cut(s) 752, 784
FaqI GGGAC 2 cut(s) 489, 955
FatI CATG 2 cut(s) 739, 962
FauNDI CATATG 1 cut(s) 433
FbaI TGATCA 1 cut(s) 186
Fnu4HI GCNGC 3 cut(s) 714, 864, 1017
FokI GGATG 5 cut(s) 8, 285, 642, 702, 1007
Fsp4HI GCNGC 3 cut(s) 714, 864, 1017
FspBI CTAG 3 cut(s) 66, 594, 626
GluI GCNGC 3 cut(s) 714, 864, 1017
HaeIII GGCC 2 cut(s) 135, 921
HapII CCGG 4 cut(s) 92, 129, 288, 907
Hin1II CATG 2 cut(s) 743, 966
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HindIII AAGCTT 2 cut(s) 259, 413
HinfI GANTC 7 cut(s) 113, 147, 229, 242, 369, 931, 1105
HpaII CCGG 4 cut(s) 92, 129, 288, 907
HphI GGTGA 2 cut(s) 122, 796
Hpy166II GTNNAC 3 cut(s) 254, 623, 796
Hpy188I TCNGA 7 cut(s) 9, 124, 228, 344, 368, 514, 1063
Hpy188III TCNNGA 3 cut(s) 386, 488, 559
Hpy8I GTNNAC 3 cut(s) 254, 623, 796
HpyAV CCTTC 3 cut(s) 35, 146, 349
HpyCH4III ACNGT 3 cut(s) 54, 710, 946
HpyCH4IV ACGT 1 cut(s) 1029
HpyCH4V TGCA 9 cut(s) 50, 174, 209, 254, 284, 497, 956, 966, 1019
HpyF10VI GCNNNNNNNGC 1 cut(s) 719
HpyF3I CTNAG 2 cut(s) 399, 978
HpySE526I ACGT 1 cut(s) 1029
Hsp92II CATG 2 cut(s) 743, 966
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 5 cut(s) 186, 484, 606, 664, 742
LmnI GCTCC 1 cut(s) 293
Lsp1109I GCAGC 3 cut(s) 700, 875, 1003
LweI GCATC 2 cut(s) 326, 620
MabI ACCWGGT 1 cut(s) 681
MaeI CTAG 3 cut(s) 66, 594, 626
MaeII ACGT 1 cut(s) 1029
MaeIII GTNAC 4 cut(s) 54, 202, 784, 949
MalI GATC 5 cut(s) 188, 486, 608, 666, 744
MboI GATC 5 cut(s) 186, 484, 606, 664, 742
MboII GAAGA 8 cut(s) 296, 312, 449, 495, 527, 793, 1068, 1076
MflI RGATCY 2 cut(s) 606, 664
MhlI GDGCHC 1 cut(s) 256
MlsI TGGCCA 1 cut(s) 921
MluNI TGGCCA 1 cut(s) 921
MlyI GAGTC 1 cut(s) 156
MmeI TCCRAC 2 cut(s) 667, 795
MnlI CCTC 3 cut(s) 125, 437, 830
Mox20I TGGCCA 1 cut(s) 921
MroXI GAANNNNTTC 2 cut(s) 767, 1057
MscI TGGCCA 1 cut(s) 921
MseI TTAA 5 cut(s) 102, 162, 759, 893, 1046
MslI CAYNNNNRTG 2 cut(s) 628, 708
Msp20I TGGCCA 1 cut(s) 921
MspA1I CMGCKG 1 cut(s) 977
MspI CCGG 4 cut(s) 92, 129, 288, 907
MspR9I CCNGG 2 cut(s) 289, 683
MvaI CCWGG 1 cut(s) 683
MwoI GCNNNNNNNGC 1 cut(s) 719
NciI CCSGG 1 cut(s) 289
NdeI CATATG 1 cut(s) 433
NdeII GATC 5 cut(s) 186, 484, 606, 664, 742
NlaIII CATG 2 cut(s) 743, 966
NlaIV GGNNCC 1 cut(s) 608
NmuCI GTSAC 2 cut(s) 202, 949
NspV TTCGAA 1 cut(s) 116
PaeR7I CTCGAG 2 cut(s) 378, 557
PceI AGGCCT 1 cut(s) 135
PdmI GAANNNNTTC 2 cut(s) 767, 1057
PfeI GAWTC 6 cut(s) 113, 229, 242, 369, 931, 1105
PkrI GCNGC 3 cut(s) 715, 865, 1018
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
Ppu21I YACGTR 1 cut(s) 1030
PshAI GACNNNNGTC 1 cut(s) 947
Psp6I CCWGG 1 cut(s) 681
PspGI CCWGG 1 cut(s) 681
PspN4I GGNNCC 1 cut(s) 608
PspPI GGNCC 4 cut(s) 214, 620, 817, 884
PspXI VCTCGAGB 1 cut(s) 378
PstNI CAGNNNCTG 1 cut(s) 1016
PsuI RGATCY 2 cut(s) 606, 664
PvuII CAGCTG 1 cut(s) 977
RseI CAYNNNNRTG 2 cut(s) 628, 708
SaqAI TTAA 5 cut(s) 102, 162, 759, 893, 1046
SatI GCNGC 3 cut(s) 714, 864, 1017
Sau3AI GATC 5 cut(s) 186, 484, 606, 664, 742
Sau96I GGNCC 4 cut(s) 214, 620, 817, 884
SchI GAGTC 1 cut(s) 156
ScrFI CCNGG 2 cut(s) 289, 683
SduI GDGCHC 1 cut(s) 256
SexAI ACCWGGT 1 cut(s) 681
SfaNI GCATC 2 cut(s) 326, 620
Sfr274I CTCGAG 2 cut(s) 378, 557
SfuI TTCGAA 1 cut(s) 116
SinI GGWCC 4 cut(s) 214, 620, 817, 884
SlaI CTCGAG 2 cut(s) 378, 557
SmiMI CAYNNNNRTG 2 cut(s) 628, 708
SmlI CTYRAG 4 cut(s) 378, 386, 557, 1115
SmoI CTYRAG 4 cut(s) 378, 386, 557, 1115
SpeI ACTAGT 1 cut(s) 65
SseBI AGGCCT 1 cut(s) 135
SsiI CCGC 1 cut(s) 887
SspMI CTAG 3 cut(s) 66, 594, 626
StuI AGGCCT 1 cut(s) 135
StyD4I CCNGG 2 cut(s) 287, 681
TaaI ACNGT 3 cut(s) 54, 710, 946
TaiI ACGT 1 cut(s) 1032
TaqI TCGA 4 cut(s) 116, 379, 558, 1079
TfiI GAWTC 6 cut(s) 113, 229, 242, 369, 931, 1105
Tru1I TTAA 5 cut(s) 102, 162, 759, 893, 1046
Tru9I TTAA 5 cut(s) 102, 162, 759, 893, 1046
TscAI CASTG 2 cut(s) 57, 958
TseFI GTSAC 2 cut(s) 202, 949
TseI GCWGC 3 cut(s) 713, 863, 1016
Tsp45I GTSAC 2 cut(s) 202, 949
TspDTI ATGAA 3 cut(s) 17, 450, 744
TspRI CASTG 2 cut(s) 57, 958
VneI GTGCAC 1 cut(s) 252
VpaK11BI GGWCC 4 cut(s) 214, 620, 817, 884
XapI RAATTY 5 cut(s) 118, 598, 673, 895, 1092
XhoI CTCGAG 2 cut(s) 378, 557
XmnI GAANNNNTTC 2 cut(s) 767, 1057
XspI CTAG 3 cut(s) 66, 594, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.