RLG00000012084

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0014665
Physical Location & Seq
Forward (+)
15074836 .. 15076311
1476 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012084

Sequence Viewer

Length: 1185 bp
ATGGCTCTGAAAGTAGTCTCTATCCTTCATTTCCTACCCTTTGTGTCTGCATTGTTACCTATAACTAGTATCGCCATCAATCCCACTTATCCGGCAGTTTTTAACTTCGGTGATTCGAATTCAGACACCGGAGGCCTTCAAGCAGGAGTCGGTTTTCCCATTAAACGCTTCAGCAATGGCTATTTGATCATAGACTATTTTGTGACTGCAATGGACCTACCCTATCTGAATCCTTACTTGGATTCAGTTGGTGCACCAAGCTTCCAAACAGGATGTAATTTTGCAACCGGGGGAGCTACCATTCTTCCAGCCAAAGTGTCTTCAACGAATCCATTTTCCTTCGGAGTTCAGGTTGCTCAATTTGTCCGATTCAAGGCTCGAGTTCTTGAGTTACTGGCTGAGAAAGGTACTAATGTTGTGCATGGATGCATGATGTGTTTGGTTTGTCATGTGGAACTTACTGATCATGAGAAGGAGCTTCAACCAGTCCTCCCATATGAAGATTATTTCAAGCAAGGCCTATACACTTTTGATGTGGGACAGAATGATCTTGATGGTGCATTTTATTCCAAATCAGAAGACCAAGTTGTTGCTTCAATTCCAGCTATTCTGACAGAACTCGAGACTGGATTTGAGAAACTATACAATCAAGGGGCTAGGAATTTTTGGATCCATAACACAGGTCCACTAGGATGCCTACCAAAAATTATAGCAACATTTGGGAAAGATCCATCAAATTTGAACCAGGTTGGATGTGTTGCTTCACATAACCGTGCTGCTGCTGCTTTCAATGAACAACTACATGATATTTGCTTGAAGTTTAAGAAAAACTTCCCTGAAGTTAATGTTACTTATGTTGACATCTTCAAGATAAAGTTGGACCTCATCACAAACTATGCTCAAAATGGTTTCAAACAAGGCATAGCAGCTTGCTGTGGGTACGGTGGTCCACCATTAAATTTTGATAACCGGATAGCTTGTGGCCAAACCAAGAATCTAAATGGGACTGTGGTCACTGCAACCCCATGCAGTAATCCAGCGGAGTATGTGAATTGGGATGGTATTCATTATACAGAAGCTGCAAATCAATACGTGTCATCGCAGATATTAACAGGAAACTACTCAGACTTCTTGAACTCTTCCATTGAATGCAAAATTTCTTATGGGAATCCAAACCTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

395

Amino Acids

43.04

Weight (kDa)

5.39

Isoelectric Point (pI)

27.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 368 3.2e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 1040
AclWI GGATC 3 cut(s) 664, 677, 722
AcoI YGGCCR 1 cut(s) 982
AcsI RAATTY 5 cut(s) 118, 661, 736, 958, 1155
AcuI CTGAAG 2 cut(s) 154, 858
AfaI GTAC 2 cut(s) 409, 941
AfiI CCNNNNNNNGG 1 cut(s) 373
AflIII ACRYGT 1 cut(s) 1092
AhlI ACTAGT 1 cut(s) 65
AjnI CCWGG 1 cut(s) 744
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 7 cut(s) 261, 296, 478, 605, 929, 977, 1079
AluI AGCT 7 cut(s) 261, 296, 478, 605, 929, 977, 1079
Alw21I GWGCWC 1 cut(s) 256
Alw26I GTCTC 2 cut(s) 22, 617
Alw44I GTGCAC 1 cut(s) 252
AlwI GGATC 3 cut(s) 664, 677, 722
AlwNI CAGNNNCTG 1 cut(s) 1079
Ama87I CYCGRG 2 cut(s) 378, 620
AoxI GGCC 3 cut(s) 133, 517, 982
ApaLI GTGCAC 1 cut(s) 252
ApeKI GCWGC 5 cut(s) 776, 779, 782, 926, 1079
ApoI RAATTY 5 cut(s) 118, 661, 736, 958, 1155
Asp700I GAANNNNTTC 1 cut(s) 830
AspS9I GGNCC 4 cut(s) 214, 683, 880, 947
AsuC2I CCSGG 1 cut(s) 289
AsuHPI GGTGA 1 cut(s) 122
AsuII TTCGAA 1 cut(s) 116
AvaI CYCGRG 2 cut(s) 378, 620
AvaII GGWCC 4 cut(s) 214, 683, 880, 947
BaeGI GKGCMC 1 cut(s) 256
BaeI ACNNNNGTAYC 4 cut(s) 399, 432, 931, 964
BalI TGGCCA 1 cut(s) 984
BamHI GGATCC 1 cut(s) 669
BbsI GAAGAC 2 cut(s) 312, 585
Bbv12I GWGCWC 1 cut(s) 256
BbvI GCAGC 5 cut(s) 763, 766, 769, 938, 1066
BccI CCATC 4 cut(s) 83, 548, 739, 1052
BciT130I CCWGG 1 cut(s) 746
BclI TGATCA 2 cut(s) 186, 463
BcnI CCSGG 1 cut(s) 289
BcoDI GTCTC 2 cut(s) 22, 617
BcuI ACTAGT 1 cut(s) 65
BfaI CTAG 3 cut(s) 66, 657, 689
BisI GCNGC 5 cut(s) 777, 780, 783, 927, 1080
BlsI GCNGC 5 cut(s) 778, 781, 784, 928, 1081
Bme1390I CCNGG 2 cut(s) 289, 746
Bme18I GGWCC 4 cut(s) 214, 683, 880, 947
BmeT110I CYCGRG 2 cut(s) 378, 620
BmgT120I GGNCC 4 cut(s) 214, 683, 880, 947
BmiI GGNNCC 1 cut(s) 671
BmrFI CCNGG 2 cut(s) 289, 746
BmsI GCATC 2 cut(s) 416, 683
BoxI GACNNNNGTC 1 cut(s) 1010
BpiI GAAGAC 2 cut(s) 312, 585
Bpu14I TTCGAA 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 1 cut(s) 289
BsaAI YACGTR 1 cut(s) 1093
BsaJI CCNNGG 1 cut(s) 288
BsaWI WCCGGW 2 cut(s) 128, 969
BsaXI ACNNNNNCTCC 4 cut(s) 336, 366, 474, 504
Bsc4I CCNNNNNNNGG 1 cut(s) 373
Bse1I ACTGG 3 cut(s) 399, 485, 631
Bse3DI GCAATG 2 cut(s) 181, 216
BseBI CCWGG 1 cut(s) 746
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 5 cut(s) 278, 431, 698, 758, 1063
BseLI CCNNNNNNNGG 1 cut(s) 373
BseMI GCAATG 2 cut(s) 181, 216
BseMII CTCAG 2 cut(s) 390, 1137
BseNI ACTGG 3 cut(s) 399, 485, 631
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 5 cut(s) 763, 766, 769, 938, 1066
BshFI GGCC 3 cut(s) 135, 519, 984
BsiHKAI GWGCWC 1 cut(s) 256
BsiHKCI CYCGRG 2 cut(s) 378, 620
BsiSI CCGG 4 cut(s) 92, 129, 288, 970
BslFI GGGAC 2 cut(s) 552, 1018
BslI CCNNNNNNNGG 1 cut(s) 373
BsmAI GTCTC 2 cut(s) 22, 617
BsmFI GGGAC 2 cut(s) 552, 1018
BsmI GAATGC 1 cut(s) 1154
BsnI GGCC 3 cut(s) 135, 519, 984
BsoBI CYCGRG 2 cut(s) 378, 620
Bsp119I TTCGAA 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 256
Bsp143I GATC 5 cut(s) 186, 463, 547, 669, 727
BspACI CCGC 1 cut(s) 1040
BspANI GGCC 3 cut(s) 135, 519, 984
BspCNI CTCAG 2 cut(s) 391, 1136
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 1 cut(s) 671
BspPI GGATC 3 cut(s) 664, 677, 722
BspT104I TTCGAA 1 cut(s) 116
BsrDI GCAATG 2 cut(s) 181, 216
BsrI ACTGG 3 cut(s) 399, 485, 631
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 5 cut(s) 186, 463, 547, 669, 727
Bst2UI CCWGG 1 cut(s) 746
Bst4CI ACNGT 3 cut(s) 773, 944, 1009
Bst6I CTCTTC 1 cut(s) 1144
BstBAI YACGTR 1 cut(s) 1093
BstBI TTCGAA 1 cut(s) 116
BstC8I GCNNGC 1 cut(s) 931
BstDEI CTNAG 2 cut(s) 399, 1123
BstF5I GGATG 5 cut(s) 278, 431, 698, 758, 1063
BstKTI GATC 5 cut(s) 189, 466, 550, 672, 730
BstMAI GTCTC 2 cut(s) 22, 617
BstMBI GATC 5 cut(s) 186, 463, 547, 669, 727
BstMWI GCNNNNNNNGC 1 cut(s) 782
BstNI CCWGG 1 cut(s) 746
BstPAI GACNNNNGTC 1 cut(s) 1010
BstSCI CCNGG 2 cut(s) 287, 744
BstSLI GKGCMC 1 cut(s) 256
BstV1I GCAGC 5 cut(s) 763, 766, 769, 938, 1066
BstV2I GAAGAC 2 cut(s) 312, 585
BstX2I RGATCY 2 cut(s) 669, 727
BstYI RGATCY 2 cut(s) 669, 727
BsuRI GGCC 3 cut(s) 135, 519, 984
BtgZI GCGATG 1 cut(s) 1083
BtsCI GGATG 5 cut(s) 278, 431, 698, 758, 1063
BtsI GCAGTG 1 cut(s) 1014
BtsIMutI CAGTG 1 cut(s) 1014
Cac8I GCNNGC 1 cut(s) 931
CaiI CAGNNNCTG 1 cut(s) 1079
CciI TCATGA 1 cut(s) 466
Cfr13I GGNCC 4 cut(s) 214, 683, 880, 947
CsiI ACCWGGT 1 cut(s) 744
Csp6I GTAC 2 cut(s) 408, 940
CviAII CATG 6 cut(s) 422, 430, 449, 467, 803, 1026
CviQI GTAC 2 cut(s) 408, 940
DdeI CTNAG 2 cut(s) 399, 1123
DpnI GATC 5 cut(s) 188, 465, 549, 671, 729
DpnII GATC 5 cut(s) 186, 463, 547, 669, 727
EaeI YGGCCR 1 cut(s) 982
Eam1104I CTCTTC 1 cut(s) 1144
EarI CTCTTC 1 cut(s) 1144
Eco147I AGGCCT 2 cut(s) 135, 519
Eco47I GGWCC 4 cut(s) 214, 683, 880, 947
Eco57I CTGAAG 2 cut(s) 154, 858
Eco88I CYCGRG 2 cut(s) 378, 620
EcoRI GAATTC 1 cut(s) 118
EcoRII CCWGG 1 cut(s) 744
EcoT22I ATGCAT 1 cut(s) 431
FaeI CATG 6 cut(s) 425, 433, 452, 470, 806, 1029
FalI AAGNNNNNCTT 2 cut(s) 815, 847
FaqI GGGAC 2 cut(s) 552, 1018
FatI CATG 6 cut(s) 421, 429, 448, 466, 802, 1025
FauNDI CATATG 1 cut(s) 496
FbaI TGATCA 2 cut(s) 186, 463
Fnu4HI GCNGC 5 cut(s) 777, 780, 783, 927, 1080
FokI GGATG 5 cut(s) 285, 438, 705, 765, 1070
Fsp4HI GCNGC 5 cut(s) 777, 780, 783, 927, 1080
FspBI CTAG 3 cut(s) 66, 657, 689
GluI GCNGC 5 cut(s) 777, 780, 783, 927, 1080
HaeIII GGCC 3 cut(s) 135, 519, 984
HapII CCGG 4 cut(s) 92, 129, 288, 970
Hin1II CATG 6 cut(s) 425, 433, 452, 470, 806, 1029
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HindIII AAGCTT 1 cut(s) 259
HinfI GANTC 8 cut(s) 113, 147, 229, 242, 328, 369, 994, 1168
HpaII CCGG 4 cut(s) 92, 129, 288, 970
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 4 cut(s) 254, 686, 859, 950
Hpy188I TCNGA 8 cut(s) 9, 124, 228, 344, 368, 577, 612, 1126
Hpy188III TCNNGA 6 cut(s) 386, 467, 551, 622, 868, 1132
Hpy8I GTNNAC 4 cut(s) 254, 686, 859, 950
HpyAV CCTTC 4 cut(s) 35, 146, 349, 466
HpyCH4III ACNGT 3 cut(s) 773, 944, 1009
HpyCH4IV ACGT 1 cut(s) 1092
HpyF10VI GCNNNNNNNGC 1 cut(s) 782
HpyF3I CTNAG 2 cut(s) 399, 1123
HpySE526I ACGT 1 cut(s) 1092
Hsp92II CATG 6 cut(s) 425, 433, 452, 470, 806, 1029
Ksp22I TGATCA 2 cut(s) 186, 463
Kzo9I GATC 5 cut(s) 186, 463, 547, 669, 727
LmnI GCTCC 2 cut(s) 293, 475
Lsp1109I GCAGC 5 cut(s) 763, 766, 769, 938, 1066
LweI GCATC 2 cut(s) 416, 683
MabI ACCWGGT 1 cut(s) 744
MaeI CTAG 3 cut(s) 66, 657, 689
MaeII ACGT 1 cut(s) 1092
MaeIII GTNAC 5 cut(s) 54, 202, 390, 847, 1012
MalI GATC 5 cut(s) 188, 465, 549, 671, 729
MboI GATC 5 cut(s) 186, 463, 547, 669, 727
MboII GAAGA 6 cut(s) 296, 312, 512, 590, 856, 1131
MflI RGATCY 2 cut(s) 669, 727
MhlI GDGCHC 1 cut(s) 256
MlsI TGGCCA 1 cut(s) 984
MluNI TGGCCA 1 cut(s) 984
MlyI GAGTC 1 cut(s) 156
MmeI TCCRAC 2 cut(s) 730, 858
MnlI CCTC 3 cut(s) 125, 500, 893
Mox20I TGGCCA 1 cut(s) 984
Mph1103I ATGCAT 1 cut(s) 431
MroXI GAANNNNTTC 1 cut(s) 830
MscI TGGCCA 1 cut(s) 984
MseI TTAA 6 cut(s) 102, 162, 822, 843, 956, 1109
MslI CAYNNNNRTG 2 cut(s) 691, 771
Msp20I TGGCCA 1 cut(s) 984
MspA1I CMGCKG 1 cut(s) 1040
MspI CCGG 4 cut(s) 92, 129, 288, 970
MspR9I CCNGG 2 cut(s) 289, 746
Mva1269I GAATGC 1 cut(s) 1154
MvaI CCWGG 1 cut(s) 746
MwoI GCNNNNNNNGC 1 cut(s) 782
NciI CCSGG 1 cut(s) 289
NdeI CATATG 1 cut(s) 496
NdeII GATC 5 cut(s) 186, 463, 547, 669, 727
NlaIII CATG 6 cut(s) 425, 433, 452, 470, 806, 1029
NlaIV GGNNCC 1 cut(s) 671
NmuCI GTSAC 2 cut(s) 202, 1012
NsiI ATGCAT 1 cut(s) 431
NspV TTCGAA 1 cut(s) 116
PaeR7I CTCGAG 2 cut(s) 378, 620
PagI TCATGA 1 cut(s) 466
PceI AGGCCT 2 cut(s) 135, 519
PctI GAATGC 1 cut(s) 1154
PdmI GAANNNNTTC 1 cut(s) 830
PfeI GAWTC 7 cut(s) 113, 229, 242, 328, 369, 994, 1168
PkrI GCNGC 5 cut(s) 778, 781, 784, 928, 1081
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
Ppu21I YACGTR 1 cut(s) 1093
PshAI GACNNNNGTC 1 cut(s) 1010
Psp6I CCWGG 1 cut(s) 744
PspGI CCWGG 1 cut(s) 744
PspN4I GGNNCC 1 cut(s) 671
PspPI GGNCC 4 cut(s) 214, 683, 880, 947
PspXI VCTCGAGB 1 cut(s) 378
PstNI CAGNNNCTG 1 cut(s) 1079
PsuI RGATCY 2 cut(s) 669, 727
RsaI GTAC 2 cut(s) 409, 941
RsaNI GTAC 2 cut(s) 408, 940
RseI CAYNNNNRTG 2 cut(s) 691, 771
SaqAI TTAA 6 cut(s) 102, 162, 822, 843, 956, 1109
SatI GCNGC 5 cut(s) 777, 780, 783, 927, 1080
Sau3AI GATC 5 cut(s) 186, 463, 547, 669, 727
Sau96I GGNCC 4 cut(s) 214, 683, 880, 947
SchI GAGTC 1 cut(s) 156
ScrFI CCNGG 2 cut(s) 289, 746
SduI GDGCHC 1 cut(s) 256
SexAI ACCWGGT 1 cut(s) 744
SfaNI GCATC 2 cut(s) 416, 683
Sfr274I CTCGAG 2 cut(s) 378, 620
SfuI TTCGAA 1 cut(s) 116
SinI GGWCC 4 cut(s) 214, 683, 880, 947
SlaI CTCGAG 2 cut(s) 378, 620
SmiMI CAYNNNNRTG 2 cut(s) 691, 771
SmlI CTYRAG 4 cut(s) 378, 386, 620, 1178
SmoI CTYRAG 4 cut(s) 378, 386, 620, 1178
SpeI ACTAGT 1 cut(s) 65
SseBI AGGCCT 2 cut(s) 135, 519
SsiI CCGC 1 cut(s) 1040
SspMI CTAG 3 cut(s) 66, 657, 689
StuI AGGCCT 2 cut(s) 135, 519
StyD4I CCNGG 2 cut(s) 287, 744
TaaI ACNGT 3 cut(s) 773, 944, 1009
TaiI ACGT 1 cut(s) 1095
TaqI TCGA 3 cut(s) 116, 379, 621
TfiI GAWTC 7 cut(s) 113, 229, 242, 328, 369, 994, 1168
Tru1I TTAA 6 cut(s) 102, 162, 822, 843, 956, 1109
Tru9I TTAA 6 cut(s) 102, 162, 822, 843, 956, 1109
TscAI CASTG 1 cut(s) 1021
TseFI GTSAC 2 cut(s) 202, 1012
TseI GCWGC 5 cut(s) 776, 779, 782, 926, 1079
Tsp45I GTSAC 2 cut(s) 202, 1012
TspDTI ATGAA 4 cut(s) 17, 513, 807, 1055
TspRI CASTG 1 cut(s) 1021
VneI GTGCAC 1 cut(s) 252
VpaK11BI GGWCC 4 cut(s) 214, 683, 880, 947
XapI RAATTY 5 cut(s) 118, 661, 736, 958, 1155
XhoI CTCGAG 2 cut(s) 378, 620
XmnI GAANNNNTTC 1 cut(s) 830
XspI CTAG 3 cut(s) 66, 657, 689
Zsp2I ATGCAT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.