FvH4_2g24080

Mitochondrial ubiquitin ligase activator of NFKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011309
Physical Location & Seq
Forward (+)
19657214 .. 19660505
3292 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24080.t2

Sequence Viewer

Length: 1029 bp
ATGCTACCTTGGGGTGGATTGAGCTGCTGTTTGAGTGCTGCTGCTCTCTATCTTCTCGGCAGAAGCAGTGGCAGGGATGCTGATATTCTTAAGACCGCCACTCGGGTTAGCCAGTTGAAGGAATTGGCTAAATTGTTGGATTCTGAGTCTATATTGCCATTGGTGGTTGCCATTTCGGGGAGAGTTAGCTCGGAAACTCCCATCACCTGTGAGTTCAGTGGTTTGAGAGGTGTAGTTGTGGAAGAAACGGCAGAACAACATTTTCTGAAACACAATGATGCTGGATCATGGATACAGGACTCTGCGTTGATGTTGTCGATGAGTAAAGAAGTCCCTTGGTATCTGGATGATGGGACTGGTCGTGTGCATGTGGTGGGAGCTCGAGGTGCCACAGGCTTTACATTGCCGGTTGCAAGTGAAGTATTTGAGGAGTCAGGGCGATCTCTTGTACGAGGGACGTTAGATTATCTCCAAGGCCTCAAGATGCTTGGAGTCAAACGAATTGAGAGGGTATTACCAACTGGAACTTCCTTGAGTGTTGTTGGTGAGGCTGTTAAAGATGACATTGGTACCATTCGAATTCAACGGCCCCACAAAGGGCCATTTTATATCTCCCCTAAAACCATTGATCAGCTCATTGCAAACCTAGGGAAATGGGCAAGGTTGTACAAATATGCGTCTTTGGGTTTGGGTGTGTTTGGTGTTTATTTGATCGCTAAGCATTCCGTCCACTACATTATGGAAAGAAGGCGGCGTTGGGAATTACAGAAAAGGGTTCTTGCTGCAGCTGCTAAAAGATCTGGGGAAGATATTGAAGGTTCAAATGATATTGAGTATAATGCATCAGAGGCTCCCAAGAAAGACCGTCTGATGCCTGATCTATGTGTGATATGCCTTGAGCAGGAGTATAATGCGGTTTTTGTCCCGTGTGGTCATATGTGCTGTTGTACAACCTGCTCCTTGCACCTGACCAATTGTCCTCTCTGCCGGCGACGGATCGAGCAGGTCGTTAAAACTTTCCGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GIDE zf-C3HC4_3

Protein Analysis

343

Amino Acids

37.81

Weight (kDa)

8.66

Isoelectric Point (pI)

57.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIDE PF12483 80 - 219 2.1e-39 E3 Ubiquitin ligase
zf-C3HC4_3 PF13920 277 - 320 2e-14 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 279 - 319 2.5e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011309)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63900
fragaria_vesca FvH4_2g24080 FvH4_2g24080
malus_domestica MD03G1270200.v1.1 MD11G1291200.v1.1
prunus_persica Prupe.8G246900_v2.0.a1
pyrus_communis pycom03g21540 pycom11g25810
rosa_chinensis RchiOBHm_Chr6g0291861
rosa_laevigata RLG00000012082
rosa_multiflora Rmu_sc0001043.1_g000019 Rmu_sc0002484.1_g000001
rosa_roxburghii Rroxscaffold_7G00175690
rosa_rugosa Rorug06G0221700
rosa_samantha Rh6AG334800 Rh6BG342300 Rh6CG348100 Rh6DG335100
rosa_wichuraiana Rw6G028920 Rw6G029140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 962, 994
Acc65I GGTACC 1 cut(s) 569
AccB1I GGYRCC 2 cut(s) 386, 569
AciI CCGC 4 cut(s) 96, 751, 914, 1021
AclWI GGATC 2 cut(s) 292, 1004
AcsI RAATTY 1 cut(s) 579
AfaI GTAC 4 cut(s) 450, 571, 668, 949
AfiI CCNNNNNNNGG 7 cut(s) 14, 102, 118, 177, 596, 597, 901
AflII CTTAAG 1 cut(s) 89
AgsI TTSAA 4 cut(s) 118, 584, 815, 822
AhdI GACNNNNNGTC 1 cut(s) 975
AjuI GAANNNNNNNTTGG 4 cut(s) 511, 543, 739, 771
AluBI AGCT 5 cut(s) 24, 189, 380, 634, 788
AluI AGCT 5 cut(s) 24, 189, 380, 634, 788
Alw21I GWGCWC 1 cut(s) 382
AlwI GGATC 2 cut(s) 292, 1004
Ama87I CYCGRG 2 cut(s) 102, 381
AoxI GGCC 3 cut(s) 475, 587, 599
ApeKI GCWGC 6 cut(s) 24, 38, 41, 782, 785, 788
ApoI RAATTY 1 cut(s) 579
Asp718I GGTACC 1 cut(s) 569
AspA2I CCTAGG 1 cut(s) 646
AspS9I GGNCC 2 cut(s) 588, 599
AsuHPI GGTGA 2 cut(s) 196, 557
AsuII TTCGAA 1 cut(s) 577
AvaI CYCGRG 2 cut(s) 102, 381
AvrII CCTAGG 1 cut(s) 646
BaeI ACNNNNGTAYC 2 cut(s) 553, 586
BanI GGYRCC 2 cut(s) 386, 569
BanII GRGCYC 1 cut(s) 382
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 6 cut(s) 11, 25, 28, 769, 775, 797
BccI CCATC 2 cut(s) 209, 344
BceAI ACGGC 2 cut(s) 264, 602
BciVI GTATCC 1 cut(s) 285
BclI TGATCA 1 cut(s) 628
BfaI CTAG 1 cut(s) 647
BfmI CTRYAG 1 cut(s) 783
BfrI CTTAAG 1 cut(s) 89
BfuAI ACCTGC 2 cut(s) 962, 994
BfuI GTATCC 1 cut(s) 285
BglII AGATCT 1 cut(s) 797
BisI GCNGC 7 cut(s) 25, 39, 42, 752, 783, 786, 789
BlnI CCTAGG 1 cut(s) 646
BlpI GCTNAGC 1 cut(s) 717
BlsI GCNGC 7 cut(s) 26, 40, 43, 753, 784, 787, 790
BmeRI GACNNNNNGTC 1 cut(s) 975
BmeT110I CYCGRG 2 cut(s) 102, 381
BmgT120I GGNCC 2 cut(s) 588, 599
BmiI GGNNCC 4 cut(s) 388, 571, 590, 852
BmsI GCATC 5 cut(s) 67, 268, 474, 851, 861
Bpu1102I GCTNAGC 1 cut(s) 717
Bpu14I TTCGAA 1 cut(s) 577
BpuEI CTTGAG 3 cut(s) 464, 553, 917
BsaJI CCNNGG 4 cut(s) 8, 335, 472, 646
Bsc4I CCNNNNNNNGG 7 cut(s) 14, 102, 118, 177, 596, 597, 901
Bse118I RCCGGY 2 cut(s) 406, 987
Bse1I ACTGG 3 cut(s) 112, 361, 526
Bse3DI GCAATG 2 cut(s) 401, 636
BseDI CCNNGG 4 cut(s) 8, 335, 472, 646
BseGI GGATG 2 cut(s) 82, 352
BseLI CCNNNNNNNGG 7 cut(s) 14, 102, 118, 177, 596, 597, 901
BseMI GCAATG 2 cut(s) 401, 636
BseMII CTCAG 1 cut(s) 135
BseNI ACTGG 3 cut(s) 112, 361, 526
BseRI GAGGAG 1 cut(s) 443
BseXI GCAGC 6 cut(s) 11, 25, 28, 769, 775, 797
BshFI GGCC 3 cut(s) 477, 589, 601
BshNI GGYRCC 2 cut(s) 386, 569
BsiHKAI GWGCWC 1 cut(s) 382
BsiHKCI CYCGRG 2 cut(s) 102, 381
BsiSI CCGG 2 cut(s) 407, 988
BslFI GGGAC 4 cut(s) 317, 367, 469, 908
BslI CCNNNNNNNGG 7 cut(s) 14, 102, 118, 177, 596, 597, 901
BsmFI GGGAC 4 cut(s) 317, 367, 469, 908
BsmI GAATGC 1 cut(s) 721
BsnI GGCC 3 cut(s) 477, 589, 601
BsoBI CYCGRG 2 cut(s) 102, 381
Bsp119I TTCGAA 1 cut(s) 577
Bsp1286I GDGCHC 1 cut(s) 382
Bsp1407I TGTACA 2 cut(s) 666, 947
Bsp143I GATC 7 cut(s) 284, 440, 628, 711, 797, 877, 996
Bsp1720I GCTNAGC 1 cut(s) 717
BspACI CCGC 4 cut(s) 96, 751, 914, 1021
BspANI GGCC 3 cut(s) 477, 589, 601
BspCNI CTCAG 1 cut(s) 136
BspLI GGNNCC 4 cut(s) 388, 571, 590, 852
BspMAI CTGCAG 1 cut(s) 787
BspMI ACCTGC 2 cut(s) 962, 994
BspPI GGATC 2 cut(s) 292, 1004
BspT104I TTCGAA 1 cut(s) 577
BspT107I GGYRCC 2 cut(s) 386, 569
BspTI CTTAAG 1 cut(s) 89
BsrDI GCAATG 2 cut(s) 401, 636
BsrFI RCCGGY 2 cut(s) 406, 987
BsrGI TGTACA 2 cut(s) 666, 947
BsrI ACTGG 3 cut(s) 112, 361, 526
BssAI RCCGGY 2 cut(s) 406, 987
BssECI CCNNGG 4 cut(s) 8, 335, 472, 646
BssMI GATC 7 cut(s) 284, 440, 628, 711, 797, 877, 996
BssT1I CCWWGG 4 cut(s) 8, 335, 472, 646
Bst4CI ACNGT 1 cut(s) 866
BstAFI CTTAAG 1 cut(s) 89
BstAUI TGTACA 2 cut(s) 666, 947
BstBI TTCGAA 1 cut(s) 577
BstC8I GCNNGC 1 cut(s) 989
BstDEI CTNAG 2 cut(s) 144, 717
BstENI CCTNNNNNAGG 1 cut(s) 899
BstF5I GGATG 2 cut(s) 82, 352
BstKTI GATC 7 cut(s) 287, 443, 631, 714, 800, 880, 999
BstMBI GATC 7 cut(s) 284, 440, 628, 711, 797, 877, 996
BstMWI GCNNNNNNNGC 3 cut(s) 386, 788, 848
BstNSI RCATGY 1 cut(s) 371
BstSFI CTRYAG 1 cut(s) 783
BstV1I GCAGC 6 cut(s) 11, 25, 28, 769, 775, 797
BstX2I RGATCY 1 cut(s) 797
BstYI RGATCY 1 cut(s) 797
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 3 cut(s) 477, 589, 601
BtsCI GGATG 2 cut(s) 82, 352
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 2 cut(s) 73, 223
BveI ACCTGC 2 cut(s) 962, 994
Cac8I GCNNGC 1 cut(s) 989
Cfr10I RCCGGY 2 cut(s) 406, 987
Cfr13I GGNCC 2 cut(s) 588, 599
CseI GACGC 1 cut(s) 666
Csp6I GTAC 4 cut(s) 449, 570, 667, 948
CviAII CATG 2 cut(s) 288, 368
CviQI GTAC 4 cut(s) 449, 570, 667, 948
DdeI CTNAG 2 cut(s) 144, 717
DpnI GATC 7 cut(s) 286, 442, 630, 713, 799, 879, 998
DpnII GATC 7 cut(s) 284, 440, 628, 711, 797, 877, 996
DriI GACNNNNNGTC 1 cut(s) 975
Eam1105I GACNNNNNGTC 1 cut(s) 975
EciI GGCGGA 1 cut(s) 1010
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 4 cut(s) 8, 335, 472, 646
Eco147I AGGCCT 1 cut(s) 477
Eco24I GRGCYC 1 cut(s) 382
Eco53kI GAGCTC 1 cut(s) 380
Eco88I CYCGRG 2 cut(s) 102, 381
EcoICRI GAGCTC 1 cut(s) 380
EcoNI CCTNNNNNAGG 1 cut(s) 899
EcoRI GAATTC 1 cut(s) 579
EcoT14I CCWWGG 4 cut(s) 8, 335, 472, 646
EcoT22I ATGCAT 1 cut(s) 844
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 4 cut(s) 8, 335, 472, 646
FaeI CATG 2 cut(s) 291, 371
FaqI GGGAC 4 cut(s) 317, 367, 469, 908
FatI CATG 2 cut(s) 287, 367
FauNDI CATATG 1 cut(s) 936
FbaI TGATCA 1 cut(s) 628
Fnu4HI GCNGC 7 cut(s) 25, 39, 42, 752, 783, 786, 789
FokI GGATG 2 cut(s) 89, 359
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 7 cut(s) 25, 39, 42, 752, 783, 786, 789
FspBI CTAG 1 cut(s) 647
GluI GCNGC 7 cut(s) 25, 39, 42, 752, 783, 786, 789
HaeIII GGCC 3 cut(s) 477, 589, 601
HapII CCGG 2 cut(s) 407, 988
HgaI GACGC 1 cut(s) 666
Hin1II CATG 2 cut(s) 291, 371
HinfI GANTC 5 cut(s) 140, 146, 299, 431, 492
HpaII CCGG 2 cut(s) 407, 988
HphI GGTGA 2 cut(s) 196, 557
Hpy166II GTNNAC 1 cut(s) 730
Hpy188I TCNGA 5 cut(s) 145, 193, 267, 847, 870
Hpy188III TCNNGA 2 cut(s) 344, 481
Hpy8I GTNNAC 1 cut(s) 730
Hpy99I CGWCG 1 cut(s) 996
HpyAV CCTTC 3 cut(s) 112, 741, 809
HpyCH4III ACNGT 1 cut(s) 866
HpyCH4IV ACGT 1 cut(s) 458
HpyCH4V TGCA 6 cut(s) 367, 413, 641, 785, 842, 964
HpyF10VI GCNNNNNNNGC 3 cut(s) 386, 788, 848
HpyF3I CTNAG 2 cut(s) 144, 717
HpySE526I ACGT 1 cut(s) 458
Hsp92II CATG 2 cut(s) 291, 371
KpnI GGTACC 1 cut(s) 573
KroI GCCGGC 1 cut(s) 987
KroNI GCCGGC 1 cut(s) 989
Ksp22I TGATCA 1 cut(s) 628
Kzo9I GATC 7 cut(s) 284, 440, 628, 711, 797, 877, 996
LmnI GCTCC 3 cut(s) 377, 856, 962
Lsp1109I GCAGC 6 cut(s) 11, 25, 28, 769, 775, 797
LweI GCATC 5 cut(s) 67, 268, 474, 851, 861
MaeI CTAG 1 cut(s) 647
MaeII ACGT 1 cut(s) 458
MalI GATC 7 cut(s) 286, 442, 630, 713, 799, 879, 998
MboI GATC 7 cut(s) 284, 440, 628, 711, 797, 877, 996
MboII GAAGA 3 cut(s) 44, 254, 818
MfeI CAATTG 1 cut(s) 973
MflI RGATCY 1 cut(s) 797
MhlI GDGCHC 1 cut(s) 382
MluCI AATT 6 cut(s) 122, 131, 501, 579, 761, 973
MlyI GAGTC 4 cut(s) 155, 293, 440, 501
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 9 cut(s) 221, 377, 421, 446, 488, 501, 541, 841, 990
Mph1103I ATGCAT 1 cut(s) 844
MroNI GCCGGC 1 cut(s) 987
MseI TTAA 3 cut(s) 90, 555, 1011
MslI CAYNNNNRTG 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 788
MspCI CTTAAG 1 cut(s) 89
MspI CCGG 2 cut(s) 407, 988
MunI CAATTG 1 cut(s) 973
Mva1269I GAATGC 1 cut(s) 721
MwoI GCNNNNNNNGC 3 cut(s) 386, 788, 848
NaeI GCCGGC 1 cut(s) 989
NdeI CATATG 1 cut(s) 936
NdeII GATC 7 cut(s) 284, 440, 628, 711, 797, 877, 996
NgoMIV GCCGGC 1 cut(s) 987
NlaIII CATG 2 cut(s) 291, 371
NlaIV GGNNCC 4 cut(s) 388, 571, 590, 852
NmeAIII GCCGAG 1 cut(s) 36
NsiI ATGCAT 1 cut(s) 844
NspI RCATGY 1 cut(s) 371
NspV TTCGAA 1 cut(s) 577
PaeR7I CTCGAG 1 cut(s) 381
PceI AGGCCT 1 cut(s) 477
PcsI WCGNNNNNNNCGW 1 cut(s) 1005
PctI GAATGC 1 cut(s) 721
PdiI GCCGGC 1 cut(s) 989
PfeI GAWTC 1 cut(s) 140
PkrI GCNGC 7 cut(s) 26, 40, 43, 753, 784, 787, 790
PleI GAGTC 4 cut(s) 154, 293, 439, 500
PpsI GAGTC 4 cut(s) 154, 293, 439, 500
Psp124BI GAGCTC 1 cut(s) 382
PspN4I GGNNCC 4 cut(s) 388, 571, 590, 852
PspPI GGNCC 2 cut(s) 588, 599
PspXI VCTCGAGB 1 cut(s) 381
PstI CTGCAG 1 cut(s) 787
PsuI RGATCY 1 cut(s) 797
PvuII CAGCTG 1 cut(s) 788
RsaI GTAC 4 cut(s) 450, 571, 668, 949
RsaNI GTAC 4 cut(s) 449, 570, 667, 948
RseI CAYNNNNRTG 1 cut(s) 276
SacI GAGCTC 1 cut(s) 382
SaqAI TTAA 3 cut(s) 90, 555, 1011
SatI GCNGC 7 cut(s) 25, 39, 42, 752, 783, 786, 789
Sau3AI GATC 7 cut(s) 284, 440, 628, 711, 797, 877, 996
Sau96I GGNCC 2 cut(s) 588, 599
SchI GAGTC 4 cut(s) 155, 293, 440, 501
SduI GDGCHC 1 cut(s) 382
SfaNI GCATC 5 cut(s) 67, 268, 474, 851, 861
SfcI CTRYAG 1 cut(s) 783
Sfr274I CTCGAG 1 cut(s) 381
SfuI TTCGAA 1 cut(s) 577
SlaI CTCGAG 1 cut(s) 381
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 5 cut(s) 89, 381, 479, 532, 896
SmoI CTYRAG 5 cut(s) 89, 381, 479, 532, 896
Sse9I AATT 6 cut(s) 122, 131, 501, 579, 761, 973
SseBI AGGCCT 1 cut(s) 477
SsiI CCGC 4 cut(s) 96, 751, 914, 1021
SspMI CTAG 1 cut(s) 647
SstI GAGCTC 1 cut(s) 382
StuI AGGCCT 1 cut(s) 477
StyI CCWWGG 4 cut(s) 8, 335, 472, 646
TaaI ACNGT 1 cut(s) 866
TaiI ACGT 1 cut(s) 461
TaqI TCGA 4 cut(s) 317, 382, 577, 999
TasI AATT 6 cut(s) 122, 131, 501, 579, 761, 973
TatI WGTACW 2 cut(s) 666, 947
TauI GCSGC 1 cut(s) 754
TfiI GAWTC 1 cut(s) 140
Tru1I TTAA 3 cut(s) 90, 555, 1011
Tru9I TTAA 3 cut(s) 90, 555, 1011
TscAI CASTG 2 cut(s) 73, 223
TseI GCWGC 6 cut(s) 24, 38, 41, 782, 785, 788
TspGWI ACGGA 2 cut(s) 715, 1009
TspRI CASTG 2 cut(s) 73, 223
Vha464I CTTAAG 1 cut(s) 89
XagI CCTNNNNNAGG 1 cut(s) 899
XapI RAATTY 1 cut(s) 579
XceI RCATGY 1 cut(s) 371
XhoI CTCGAG 1 cut(s) 381
XmaJI CCTAGG 1 cut(s) 646
XspI CTAG 1 cut(s) 647
Zsp2I ATGCAT 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.