MD03G1270200.v1.1

Mitochondrial ubiquitin ligase activator of NFKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011309
Physical Location & Seq
Forward (+)
35412063 .. 35415775
3713 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1270200.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGCTACCTTGGGGTGGATTGAGTTGTTGTCTGAGTGGAGCTGCTCTTTACCTTCTTGGCAGGAGCAGTGGCAGGGATGCAGAGATTCTTAAATCCGCCACTCGAATTAATCAGTTGAAGGAATTGGCAAAATTGTTGGATTCCGAATATGTATTGCCATTGGTGGTTGCGGTCTCGGGGAGAGTTAGCTCTGAAACCCCCATCAGCTGTGAGTTTACTGGTTTACGAGGCGTAGTTGTGGAGGAAACGGCAGAACAACATTTTTTGAAGCACAATGATGCTGGCTCATGGATACAGGATTCTGCTTTGATGCTTTCAATGAGTAAAGAAGTCCCCTGGTACTTGGATGATGGAACTGGTCGCGTGTTTGTGGTGGGAGCTCGAGCTGCCACAGGATTTGTATTGCCGGTTGCAAGCGAGGTATTTGAAGAGTCAGGACGATCCCTCGTACGTGGGACATTAGATTATCTCCAAGGCCTCAAGATGCTTGGAGTCAAACGAATTGAGAGGGTACTTCCAACTGGTACTTCTATGAGTGTTGTTGGTGAGGCTGTCAAAGATGACATTGGTACAATTCGGATTCAACGACCTCAGAAAGGGCCATTTTATGTCTCTCCCAAAACCATTGATCAGCTTATTTCAAACCTTGGAAAATGGGCAAGGTGGTACAAATATGCATCTATGGGTTTGACCGTGTTTGGTGTTTATTTGGTTGCAAAGCATTCTATCCAATACTTCATGGAGAGAAAGCGGCGTAGGGAATTGCAGAAAAGGGTTCTTGCTGCAGCTGCTAAAAGATCTGGGGAAGATAGTGAAGGTTCAAACAAAAAAGATGACAATGCATCAGATGGAAGCAAGAGAGACCGTATGATGCCAAATTTATGTGTGATATGCCTTGAGCAGGAGTACAATGCGGTTTTTGTCCCGTGCGGTCATATGTGCTGTTGTACAACTTGCTCCTTACACCTGACCAGTTGCCCTCTCTGCCGGAGACGAATTGATCAGGCTGTGAAGACTTTCCGTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GIDE zf-C3HC4_3

Protein Analysis

343

Amino Acids

37.9

Weight (kDa)

9.02

Isoelectric Point (pI)

55.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIDE PF12483 85 - 234 6.4e-40 E3 Ubiquitin ligase
zf-C3HC4_3 PF13920 293 - 335 4.2e-14 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 295 - 336 9.6e-08 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011309)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63900
fragaria_vesca FvH4_2g24080 FvH4_2g24080
malus_domestica MD03G1270200.v1.1 MD11G1291200.v1.1
prunus_persica Prupe.8G246900_v2.0.a1
pyrus_communis pycom03g21540 pycom11g25810
rosa_chinensis RchiOBHm_Chr6g0291861
rosa_laevigata RLG00000012082
rosa_multiflora Rmu_sc0001043.1_g000019 Rmu_sc0002484.1_g000001
rosa_roxburghii Rroxscaffold_7G00175690
rosa_rugosa Rorug06G0221700
rosa_samantha Rh6AG334800 Rh6BG342300 Rh6CG348100 Rh6DG335100
rosa_wichuraiana Rw6G028920 Rw6G029140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 363
AciI CCGC 5 cut(s) 96, 170, 751, 914, 930
AclWI GGATC 1 cut(s) 435
AcsI RAATTY 1 cut(s) 877
AfaI GTAC 8 cut(s) 341, 450, 513, 526, 571, 668, 908, 949
AfiI CCNNNNNNNGG 3 cut(s) 14, 596, 901
AgsI TTSAA 7 cut(s) 118, 268, 318, 428, 584, 642, 822
AhdI GACNNNNNGTC 1 cut(s) 1020
AjnI CCWGG 1 cut(s) 335
AjuI GAANNNNNNNTTGG 2 cut(s) 511, 543
AluBI AGCT 7 cut(s) 41, 189, 207, 380, 386, 634, 788
AluI AGCT 7 cut(s) 41, 189, 207, 380, 386, 634, 788
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 4 cut(s) 178, 616, 855, 985
AlwI GGATC 1 cut(s) 435
Ama87I CYCGRG 2 cut(s) 175, 381
AoxI GGCC 2 cut(s) 475, 599
ApeKI GCWGC 5 cut(s) 41, 386, 782, 785, 788
ApoI RAATTY 1 cut(s) 877
AseI ATTAAT 1 cut(s) 108
Asp700I GAANNNNTTC 1 cut(s) 1016
AspS9I GGNCC 1 cut(s) 599
AsuHPI GGTGA 1 cut(s) 557
AvaI CYCGRG 2 cut(s) 175, 381
BanII GRGCYC 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 1019
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 5 cut(s) 28, 373, 769, 775, 797
BccI CCATC 3 cut(s) 209, 344, 842
BceAI ACGGC 1 cut(s) 264
BciT130I CCWGG 1 cut(s) 337
BciVI GTATCC 1 cut(s) 285
BclI TGATCA 2 cut(s) 628, 1000
BcoDI GTCTC 4 cut(s) 178, 616, 855, 985
BfmI CTRYAG 1 cut(s) 783
BfuI GTATCC 1 cut(s) 285
BglII AGATCT 1 cut(s) 797
BisI GCNGC 6 cut(s) 42, 387, 752, 783, 786, 789
BlsI GCNGC 6 cut(s) 43, 388, 753, 784, 787, 790
Bme1390I CCNGG 1 cut(s) 337
BmeRI GACNNNNNGTC 1 cut(s) 1020
BmeT110I CYCGRG 2 cut(s) 175, 381
BmgT120I GGNCC 1 cut(s) 599
BmrFI CCNGG 1 cut(s) 337
BmsI GCATC 7 cut(s) 67, 268, 300, 474, 686, 851, 861
BpiI GAAGAC 1 cut(s) 1019
BpuEI CTTGAG 2 cut(s) 464, 917
BsaAI YACGTR 1 cut(s) 452
BsaI GGTCTC 2 cut(s) 178, 855
BsaJI CCNNGG 4 cut(s) 8, 335, 472, 646
Bsc4I CCNNNNNNNGG 3 cut(s) 14, 596, 901
Bse118I RCCGGY 1 cut(s) 406
Bse1I ACTGG 4 cut(s) 223, 361, 526, 972
BseBI CCWGG 1 cut(s) 337
BseDI CCNNGG 4 cut(s) 8, 335, 472, 646
BseGI GGATG 2 cut(s) 82, 352
BseLI CCNNNNNNNGG 3 cut(s) 14, 596, 901
BseMII CTCAG 2 cut(s) 23, 605
BseNI ACTGG 4 cut(s) 223, 361, 526, 972
BseXI GCAGC 5 cut(s) 28, 373, 769, 775, 797
Bsh1236I CGCG 1 cut(s) 363
BshFI GGCC 2 cut(s) 477, 601
BsiHKAI GWGCWC 1 cut(s) 382
BsiHKCI CYCGRG 2 cut(s) 175, 381
BsiSI CCGG 2 cut(s) 407, 988
BsiWI CGTACG 1 cut(s) 448
BslFI GGGAC 3 cut(s) 317, 469, 908
BslI CCNNNNNNNGG 3 cut(s) 14, 596, 901
BsmAI GTCTC 4 cut(s) 178, 616, 855, 985
BsmBI CGTCTC 1 cut(s) 985
BsmFI GGGAC 3 cut(s) 317, 469, 908
BsmI GAATGC 1 cut(s) 721
BsnI GGCC 2 cut(s) 477, 601
Bso31I GGTCTC 2 cut(s) 178, 855
BsoBI CYCGRG 2 cut(s) 175, 381
Bsp1286I GDGCHC 1 cut(s) 382
Bsp1407I TGTACA 1 cut(s) 947
Bsp143I GATC 4 cut(s) 440, 628, 797, 1000
BspACI CCGC 5 cut(s) 96, 170, 751, 914, 930
BspANI GGCC 2 cut(s) 477, 601
BspCNI CTCAG 2 cut(s) 24, 604
BspFNI CGCG 1 cut(s) 363
BspMAI CTGCAG 1 cut(s) 787
BspPI GGATC 1 cut(s) 435
BspTNI GGTCTC 2 cut(s) 178, 855
BsrFI RCCGGY 1 cut(s) 406
BsrGI TGTACA 1 cut(s) 947
BsrI ACTGG 4 cut(s) 223, 361, 526, 972
BssAI RCCGGY 1 cut(s) 406
BssECI CCNNGG 4 cut(s) 8, 335, 472, 646
BssMI GATC 4 cut(s) 440, 628, 797, 1000
BssT1I CCWWGG 3 cut(s) 8, 472, 646
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 2 cut(s) 694, 866
Bst6I CTCTTC 1 cut(s) 423
BstAUI TGTACA 1 cut(s) 947
BstBAI YACGTR 1 cut(s) 452
BstC8I GCNNGC 2 cut(s) 283, 415
BstDEI CTNAG 2 cut(s) 32, 591
BstENI CCTNNNNNAGG 2 cut(s) 594, 899
BstF5I GGATG 2 cut(s) 82, 352
BstFNI CGCG 1 cut(s) 363
BstKTI GATC 4 cut(s) 443, 631, 800, 1003
BstMAI GTCTC 4 cut(s) 178, 616, 855, 985
BstMBI GATC 4 cut(s) 440, 628, 797, 1000
BstMWI GCNNNNNNNGC 3 cut(s) 386, 788, 984
BstNI CCWGG 1 cut(s) 337
BstSCI CCNGG 1 cut(s) 335
BstSFI CTRYAG 1 cut(s) 783
BstUI CGCG 1 cut(s) 363
BstV1I GCAGC 5 cut(s) 28, 373, 769, 775, 797
BstV2I GAAGAC 1 cut(s) 1019
BstX2I RGATCY 1 cut(s) 797
BstYI RGATCY 1 cut(s) 797
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 2 cut(s) 477, 601
BtsCI GGATG 2 cut(s) 82, 352
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 1 cut(s) 73
Cac8I GCNNGC 2 cut(s) 283, 415
Cfr10I RCCGGY 1 cut(s) 406
Cfr13I GGNCC 1 cut(s) 599
Csp6I GTAC 8 cut(s) 340, 449, 512, 525, 570, 667, 907, 948
CspCI CAANNNNNGTGG 2 cut(s) 379, 414
CviAII CATG 2 cut(s) 288, 739
CviQI GTAC 8 cut(s) 340, 449, 512, 525, 570, 667, 907, 948
DdeI CTNAG 2 cut(s) 32, 591
DpnI GATC 4 cut(s) 442, 630, 799, 1002
DpnII GATC 4 cut(s) 440, 628, 797, 1000
DriI GACNNNNNGTC 1 cut(s) 1020
Eam1104I CTCTTC 1 cut(s) 423
Eam1105I GACNNNNNGTC 1 cut(s) 1020
EarI CTCTTC 1 cut(s) 423
EciI GGCGGA 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 3 cut(s) 8, 472, 646
Eco147I AGGCCT 1 cut(s) 477
Eco24I GRGCYC 1 cut(s) 382
Eco31I GGTCTC 2 cut(s) 178, 855
Eco53kI GAGCTC 1 cut(s) 380
Eco88I CYCGRG 2 cut(s) 175, 381
EcoICRI GAGCTC 1 cut(s) 380
EcoNI CCTNNNNNAGG 2 cut(s) 594, 899
EcoRII CCWGG 1 cut(s) 335
EcoT14I CCWWGG 3 cut(s) 8, 472, 646
EcoT22I ATGCAT 2 cut(s) 679, 844
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 3 cut(s) 8, 472, 646
Esp3I CGTCTC 1 cut(s) 985
FaeI CATG 2 cut(s) 291, 742
FaqI GGGAC 3 cut(s) 317, 469, 908
FatI CATG 2 cut(s) 287, 738
FauNDI CATATG 1 cut(s) 936
FbaI TGATCA 2 cut(s) 628, 1000
Fnu4HI GCNGC 6 cut(s) 42, 387, 752, 783, 786, 789
FokI GGATG 2 cut(s) 89, 359
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 6 cut(s) 42, 387, 752, 783, 786, 789
GluI GCNGC 6 cut(s) 42, 387, 752, 783, 786, 789
HaeIII GGCC 2 cut(s) 477, 601
HapII CCGG 2 cut(s) 407, 988
Hin1II CATG 2 cut(s) 291, 742
HinfI GANTC 6 cut(s) 85, 140, 299, 431, 492, 580
HpaII CCGG 2 cut(s) 407, 988
HphI GGTGA 1 cut(s) 557
Hpy166II GTNNAC 2 cut(s) 216, 224
Hpy188I TCNGA 6 cut(s) 33, 145, 193, 579, 594, 847
Hpy188III TCNNGA 2 cut(s) 435, 481
Hpy8I GTNNAC 2 cut(s) 216, 224
HpyAV CCTTC 3 cut(s) 62, 112, 809
HpyCH4III ACNGT 2 cut(s) 694, 866
HpyCH4IV ACGT 1 cut(s) 451
HpyCH4V TGCA 7 cut(s) 80, 413, 677, 716, 766, 785, 842
HpyF10VI GCNNNNNNNGC 3 cut(s) 386, 788, 984
HpyF3I CTNAG 2 cut(s) 32, 591
HpySE526I ACGT 1 cut(s) 451
Hsp92II CATG 2 cut(s) 291, 742
Ksp22I TGATCA 2 cut(s) 628, 1000
Kzo9I GATC 4 cut(s) 440, 628, 797, 1000
LmnI GCTCC 4 cut(s) 38, 63, 377, 962
Lsp1109I GCAGC 5 cut(s) 28, 373, 769, 775, 797
LweI GCATC 7 cut(s) 67, 268, 300, 474, 686, 851, 861
MaeII ACGT 1 cut(s) 451
MalI GATC 4 cut(s) 442, 630, 799, 1002
MboI GATC 4 cut(s) 440, 628, 797, 1000
MboII GAAGA 3 cut(s) 440, 818, 1024
MflI RGATCY 1 cut(s) 797
MhlI GDGCHC 1 cut(s) 382
MluCI AATT 8 cut(s) 105, 122, 131, 501, 573, 761, 877, 996
MlyI GAGTC 2 cut(s) 440, 501
MmeI TCCRAC 2 cut(s) 117, 542
MnlI CCTC 9 cut(s) 221, 235, 412, 455, 488, 501, 541, 600, 990
Mph1103I ATGCAT 2 cut(s) 679, 844
MroXI GAANNNNTTC 1 cut(s) 1016
MseI TTAA 3 cut(s) 90, 108, 1027
MslI CAYNNNNRTG 1 cut(s) 276
MspA1I CMGCKG 2 cut(s) 207, 788
MspI CCGG 2 cut(s) 407, 988
MspR9I CCNGG 1 cut(s) 337
Mva1269I GAATGC 1 cut(s) 721
MvaI CCWGG 1 cut(s) 337
MvnI CGCG 1 cut(s) 363
MwoI GCNNNNNNNGC 3 cut(s) 386, 788, 984
NdeI CATATG 1 cut(s) 936
NdeII GATC 4 cut(s) 440, 628, 797, 1000
NlaIII CATG 2 cut(s) 291, 742
NsiI ATGCAT 2 cut(s) 679, 844
PaeR7I CTCGAG 1 cut(s) 381
PceI AGGCCT 1 cut(s) 477
PcsI WCGNNNNNNNCGW 1 cut(s) 583
PctI GAATGC 1 cut(s) 721
PdmI GAANNNNTTC 1 cut(s) 1016
PfeI GAWTC 4 cut(s) 85, 140, 299, 580
Pfl23II CGTACG 1 cut(s) 448
PkrI GCNGC 6 cut(s) 43, 388, 753, 784, 787, 790
PleI GAGTC 2 cut(s) 439, 500
PpsI GAGTC 2 cut(s) 439, 500
Ppu21I YACGTR 1 cut(s) 452
PshBI ATTAAT 1 cut(s) 108
Psp124BI GAGCTC 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 335
PspGI CCWGG 1 cut(s) 335
PspLI CGTACG 1 cut(s) 448
PspPI GGNCC 1 cut(s) 599
PspXI VCTCGAGB 1 cut(s) 381
PstI CTGCAG 1 cut(s) 787
PsuI RGATCY 1 cut(s) 797
PvuII CAGCTG 2 cut(s) 207, 788
RsaI GTAC 8 cut(s) 341, 450, 513, 526, 571, 668, 908, 949
RsaNI GTAC 8 cut(s) 340, 449, 512, 525, 570, 667, 907, 948
RseI CAYNNNNRTG 1 cut(s) 276
SacI GAGCTC 1 cut(s) 382
SaqAI TTAA 3 cut(s) 90, 108, 1027
SatI GCNGC 6 cut(s) 42, 387, 752, 783, 786, 789
Sau3AI GATC 4 cut(s) 440, 628, 797, 1000
Sau96I GGNCC 1 cut(s) 599
SchI GAGTC 2 cut(s) 440, 501
ScrFI CCNGG 1 cut(s) 337
SduI GDGCHC 1 cut(s) 382
SfaNI GCATC 7 cut(s) 67, 268, 300, 474, 686, 851, 861
SfcI CTRYAG 1 cut(s) 783
Sfr274I CTCGAG 1 cut(s) 381
SlaI CTCGAG 1 cut(s) 381
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 3 cut(s) 381, 479, 896
SmoI CTYRAG 3 cut(s) 381, 479, 896
Sse9I AATT 8 cut(s) 105, 122, 131, 501, 573, 761, 877, 996
SseBI AGGCCT 1 cut(s) 477
SsiI CCGC 5 cut(s) 96, 170, 751, 914, 930
SstI GAGCTC 1 cut(s) 382
StuI AGGCCT 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 335
StyI CCWWGG 3 cut(s) 8, 472, 646
TaaI ACNGT 2 cut(s) 694, 866
TaiI ACGT 1 cut(s) 454
TaqI TCGA 2 cut(s) 103, 382
TasI AATT 8 cut(s) 105, 122, 131, 501, 573, 761, 877, 996
TatI WGTACW 2 cut(s) 906, 947
TauI GCSGC 1 cut(s) 754
TfiI GAWTC 4 cut(s) 85, 140, 299, 580
Tru1I TTAA 3 cut(s) 90, 108, 1027
Tru9I TTAA 3 cut(s) 90, 108, 1027
TscAI CASTG 1 cut(s) 73
TseI GCWGC 5 cut(s) 41, 386, 782, 785, 788
TspDTI ATGAA 1 cut(s) 727
TspGWI ACGGA 1 cut(s) 1010
TspRI CASTG 1 cut(s) 73
VspI ATTAAT 1 cut(s) 108
XagI CCTNNNNNAGG 2 cut(s) 594, 899
XapI RAATTY 1 cut(s) 877
XhoI CTCGAG 1 cut(s) 381
XmnI GAANNNNTTC 1 cut(s) 1016
Zsp2I ATGCAT 2 cut(s) 679, 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.