pycom03g21540

Mitochondrial ubiquitin ligase activator of NFKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0011309
Physical Location & Seq
Forward (+)
21835678 .. 21838438
2761 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g21540.2

Sequence Viewer

Length: 1002 bp
ATGATTTCTGTCTGGGTCCGGTTTTTGAGCTCTGACGCTTCCGGAGCAGTGGCAGAGATTCTTAAATCCACCACTCGAATTAATCAGTTGAAGGAATTGGCAAAATTGTTGGATTCCGAATGTGTATTGCCATTGGTAGTTGCGGTCTCAGGGAGAGTTAGCTCTGAAACCCCCATCAACTGTGAGTTCACTGGTCTAAGAGGCGTAGTTGTGGAGGAAACGGCAGAACAGCATTTTCTGAAACACAATGATGCTGGCTCATGGATACAGGATTCTGCTTTGATGCTTTCAATGAGTAAAGAAGTCCCCTGGTACTTGGATGATGGAACTGGTCGCGTGTTTGTGGTGGGAGCTCGAGCTGCCACAGGATTTCTATTGCCGGTTGCAAGTGAGGTATTTGAAGAGTCAGGACGATCCCTTGTACGTGGGACATTAGATTATCTCCAAGGCCTCAAGATGCTTGGAGTCAAACGAATTGAGAGGGTACTTCCAACCGGTACTTCTATGAGTGTTGTTGGTGAGGCTGTCAAAGATGACATTGGTACAATTCGGATTCAACGACCTCAGAAAGGGCCATTTTATGTCTCTCCCAAGACCATTGATCAGCTTATTGCAAACCTTGGAAAATGGGCAAGGTGGTACAAATATGCATCCATGGGTTTGACTGTGTTTGGTGTTTATTTGGTTGCTAAGCATTCTATCCAATACATCATGGAGAGAAAGCGGCGTAGGGAATTGCAGAAAAGGGTTCTCGCTGCAGCTGCTAAAAGATCTGGGGAAGATAGTGAAGGTTCAAACGAAAAGGATGATAATGCATTAGATGGAAGCAAGAGAGACCGTATGATGCCAAATTTATGTGTGATATGCCTTGAGCAGGAGTACAATGCGGTTTTTGTCCCGTGCGGTCATATGTGCTGTTGTACAACTTGCTCCTTACACCTGACCAGTTGCCCTCTCTGCCGGAGACGAATTGATCAGTCTGTGAAGACTTTCCGTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GIDE zf-C3HC4_3

Protein Analysis

334

Amino Acids

37.04

Weight (kDa)

8.82

Isoelectric Point (pI)

53.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011309)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63900
fragaria_vesca FvH4_2g24080 FvH4_2g24080
malus_domestica MD03G1270200.v1.1 MD11G1291200.v1.1
prunus_persica Prupe.8G246900_v2.0.a1
pyrus_communis pycom03g21540 pycom11g25810
rosa_chinensis RchiOBHm_Chr6g0291861
rosa_laevigata RLG00000012082
rosa_multiflora Rmu_sc0001043.1_g000019 Rmu_sc0002484.1_g000001
rosa_roxburghii Rroxscaffold_7G00175690
rosa_rugosa Rorug06G0221700
rosa_samantha Rh6AG334800 Rh6BG342300 Rh6CG348100 Rh6DG335100
rosa_wichuraiana Rw6G028920 Rw6G029140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 336
AccIII TCCGGA 1 cut(s) 41
AciI CCGC 4 cut(s) 143, 724, 887, 903
AclWI GGATC 1 cut(s) 408
AcsI RAATTY 1 cut(s) 850
AfaI GTAC 8 cut(s) 314, 423, 486, 499, 544, 641, 881, 922
AfiI CCNNNNNNNGG 2 cut(s) 569, 874
AgeI ACCGGT 1 cut(s) 494
AgsI TTSAA 5 cut(s) 91, 291, 401, 557, 795
AhdI GACNNNNNGTC 1 cut(s) 993
AjnI CCWGG 1 cut(s) 308
AjuI GAANNNNNNNTTGG 2 cut(s) 484, 516
AluBI AGCT 6 cut(s) 30, 162, 353, 359, 607, 761
AluI AGCT 6 cut(s) 30, 162, 353, 359, 607, 761
Alw21I GWGCWC 2 cut(s) 32, 355
Alw26I GTCTC 4 cut(s) 151, 589, 828, 958
AlwI GGATC 1 cut(s) 408
Ama87I CYCGRG 1 cut(s) 354
Aor13HI TCCGGA 1 cut(s) 41
AoxI GGCC 2 cut(s) 448, 572
ApeKI GCWGC 4 cut(s) 359, 755, 758, 761
ApoI RAATTY 1 cut(s) 850
AseI ATTAAT 1 cut(s) 81
AsiGI ACCGGT 1 cut(s) 494
Asp700I GAANNNNTTC 1 cut(s) 989
AspS9I GGNCC 2 cut(s) 16, 572
AsuHPI GGTGA 1 cut(s) 530
AvaI CYCGRG 1 cut(s) 354
AvaII GGWCC 1 cut(s) 16
BanII GRGCYC 2 cut(s) 32, 355
BbsI GAAGAC 1 cut(s) 992
Bbv12I GWGCWC 2 cut(s) 32, 355
BbvI GCAGC 4 cut(s) 346, 742, 748, 770
BccI CCATC 3 cut(s) 182, 317, 815
BceAI ACGGC 1 cut(s) 237
BciT130I CCWGG 1 cut(s) 310
BciVI GTATCC 1 cut(s) 258
BclI TGATCA 2 cut(s) 601, 973
BcoDI GTCTC 4 cut(s) 151, 589, 828, 958
BfmI CTRYAG 1 cut(s) 756
BfuI GTATCC 1 cut(s) 258
BglII AGATCT 1 cut(s) 770
BisI GCNGC 5 cut(s) 360, 725, 756, 759, 762
BlpI GCTNAGC 1 cut(s) 690
BlsI GCNGC 5 cut(s) 361, 726, 757, 760, 763
Bme1390I CCNGG 1 cut(s) 310
Bme18I GGWCC 1 cut(s) 16
BmeRI GACNNNNNGTC 1 cut(s) 993
BmeT110I CYCGRG 1 cut(s) 354
BmgT120I GGNCC 2 cut(s) 16, 572
BmiI GGNNCC 1 cut(s) 17
BmrFI CCNGG 1 cut(s) 310
BmsI GCATC 5 cut(s) 241, 273, 447, 659, 834
BpiI GAAGAC 1 cut(s) 992
Bpu1102I GCTNAGC 1 cut(s) 690
BpuEI CTTGAG 2 cut(s) 437, 890
BsaAI YACGTR 1 cut(s) 425
BsaI GGTCTC 2 cut(s) 151, 828
BsaJI CCNNGG 4 cut(s) 308, 445, 619, 654
BsaWI WCCGGW 3 cut(s) 18, 41, 494
Bsc4I CCNNNNNNNGG 2 cut(s) 569, 874
Bse118I RCCGGY 2 cut(s) 379, 494
Bse1I ACTGG 3 cut(s) 196, 334, 945
BseAI TCCGGA 1 cut(s) 41
BseBI CCWGG 1 cut(s) 310
BseDI CCNNGG 4 cut(s) 308, 445, 619, 654
BseGI GGATG 3 cut(s) 325, 650, 811
BseLI CCNNNNNNNGG 2 cut(s) 569, 874
BseMII CTCAG 2 cut(s) 162, 578
BseNI ACTGG 3 cut(s) 196, 334, 945
BseXI GCAGC 4 cut(s) 346, 742, 748, 770
Bsh1236I CGCG 1 cut(s) 336
BshFI GGCC 2 cut(s) 450, 574
BshTI ACCGGT 1 cut(s) 494
BsiHKAI GWGCWC 2 cut(s) 32, 355
BsiHKCI CYCGRG 1 cut(s) 354
BsiSI CCGG 5 cut(s) 19, 42, 380, 495, 961
BslFI GGGAC 3 cut(s) 290, 442, 881
BslI CCNNNNNNNGG 2 cut(s) 569, 874
BsmAI GTCTC 4 cut(s) 151, 589, 828, 958
BsmBI CGTCTC 1 cut(s) 958
BsmFI GGGAC 3 cut(s) 290, 442, 881
BsmI GAATGC 1 cut(s) 694
BsnI GGCC 2 cut(s) 450, 574
Bso31I GGTCTC 2 cut(s) 151, 828
BsoBI CYCGRG 1 cut(s) 354
Bsp1286I GDGCHC 2 cut(s) 32, 355
Bsp13I TCCGGA 1 cut(s) 41
Bsp1407I TGTACA 1 cut(s) 920
Bsp143I GATC 4 cut(s) 413, 601, 770, 973
Bsp1720I GCTNAGC 1 cut(s) 690
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 4 cut(s) 143, 724, 887, 903
BspANI GGCC 2 cut(s) 450, 574
BspCNI CTCAG 2 cut(s) 161, 577
BspEI TCCGGA 1 cut(s) 41
BspFNI CGCG 1 cut(s) 336
BspLI GGNNCC 1 cut(s) 17
BspMAI CTGCAG 1 cut(s) 760
BspPI GGATC 1 cut(s) 408
BspTNI GGTCTC 2 cut(s) 151, 828
BsrFI RCCGGY 2 cut(s) 379, 494
BsrGI TGTACA 1 cut(s) 920
BsrI ACTGG 3 cut(s) 196, 334, 945
BssAI RCCGGY 2 cut(s) 379, 494
BssECI CCNNGG 4 cut(s) 308, 445, 619, 654
BssMI GATC 4 cut(s) 413, 601, 770, 973
BssT1I CCWWGG 3 cut(s) 445, 619, 654
Bst2UI CCWGG 1 cut(s) 310
Bst4CI ACNGT 3 cut(s) 182, 667, 839
Bst6I CTCTTC 1 cut(s) 396
BstAUI TGTACA 1 cut(s) 920
BstBAI YACGTR 1 cut(s) 425
BstC8I GCNNGC 1 cut(s) 256
BstDEI CTNAG 4 cut(s) 148, 197, 564, 690
BstDSI CCRYGG 1 cut(s) 654
BstENI CCTNNNNNAGG 2 cut(s) 567, 872
BstF5I GGATG 3 cut(s) 325, 650, 811
BstFNI CGCG 1 cut(s) 336
BstKTI GATC 4 cut(s) 416, 604, 773, 976
BstMAI GTCTC 4 cut(s) 151, 589, 828, 958
BstMBI GATC 4 cut(s) 413, 601, 770, 973
BstMWI GCNNNNNNNGC 4 cut(s) 44, 359, 761, 957
BstNI CCWGG 1 cut(s) 310
BstSCI CCNGG 1 cut(s) 308
BstSFI CTRYAG 1 cut(s) 756
BstUI CGCG 1 cut(s) 336
BstV1I GCAGC 4 cut(s) 346, 742, 748, 770
BstV2I GAAGAC 1 cut(s) 992
BstX2I RGATCY 1 cut(s) 770
BstYI RGATCY 1 cut(s) 770
BsuI GTATCC 1 cut(s) 258
BsuRI GGCC 2 cut(s) 450, 574
BtgI CCRYGG 1 cut(s) 654
BtsCI GGATG 3 cut(s) 325, 650, 811
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 2 cut(s) 54, 189
Cac8I GCNNGC 1 cut(s) 256
Cfr10I RCCGGY 2 cut(s) 379, 494
Cfr13I GGNCC 2 cut(s) 16, 572
CseI GACGC 1 cut(s) 44
Csp6I GTAC 8 cut(s) 313, 422, 485, 498, 543, 640, 880, 921
CspAI ACCGGT 1 cut(s) 494
CviAII CATG 3 cut(s) 261, 655, 712
CviQI GTAC 8 cut(s) 313, 422, 485, 498, 543, 640, 880, 921
DdeI CTNAG 4 cut(s) 148, 197, 564, 690
DpnI GATC 4 cut(s) 415, 603, 772, 975
DpnII GATC 4 cut(s) 413, 601, 770, 973
DriI GACNNNNNGTC 1 cut(s) 993
Eam1104I CTCTTC 1 cut(s) 396
Eam1105I GACNNNNNGTC 1 cut(s) 993
EarI CTCTTC 1 cut(s) 396
Ecl136II GAGCTC 2 cut(s) 30, 353
Eco130I CCWWGG 3 cut(s) 445, 619, 654
Eco147I AGGCCT 1 cut(s) 450
Eco24I GRGCYC 2 cut(s) 32, 355
Eco31I GGTCTC 2 cut(s) 151, 828
Eco47I GGWCC 1 cut(s) 16
Eco53kI GAGCTC 2 cut(s) 30, 353
Eco88I CYCGRG 1 cut(s) 354
EcoICRI GAGCTC 2 cut(s) 30, 353
EcoNI CCTNNNNNAGG 2 cut(s) 567, 872
EcoRII CCWGG 1 cut(s) 308
EcoT14I CCWWGG 3 cut(s) 445, 619, 654
EcoT22I ATGCAT 2 cut(s) 652, 817
EcoT38I GRGCYC 2 cut(s) 32, 355
ErhI CCWWGG 3 cut(s) 445, 619, 654
Esp3I CGTCTC 1 cut(s) 958
FaeI CATG 3 cut(s) 264, 658, 715
FaqI GGGAC 3 cut(s) 290, 442, 881
FatI CATG 3 cut(s) 260, 654, 711
FauNDI CATATG 1 cut(s) 909
FbaI TGATCA 2 cut(s) 601, 973
Fnu4HI GCNGC 5 cut(s) 360, 725, 756, 759, 762
FokI GGATG 3 cut(s) 332, 637, 818
FriOI GRGCYC 2 cut(s) 32, 355
Fsp4HI GCNGC 5 cut(s) 360, 725, 756, 759, 762
GluI GCNGC 5 cut(s) 360, 725, 756, 759, 762
HaeIII GGCC 2 cut(s) 450, 574
HapII CCGG 5 cut(s) 19, 42, 380, 495, 961
HgaI GACGC 1 cut(s) 44
Hin1II CATG 3 cut(s) 264, 658, 715
HinfI GANTC 6 cut(s) 58, 113, 272, 404, 465, 553
HpaII CCGG 5 cut(s) 19, 42, 380, 495, 961
HphI GGTGA 1 cut(s) 530
Hpy166II GTNNAC 1 cut(s) 189
Hpy188I TCNGA 6 cut(s) 34, 118, 166, 240, 552, 567
Hpy188III TCNNGA 3 cut(s) 42, 408, 454
Hpy8I GTNNAC 1 cut(s) 189
HpyAV CCTTC 2 cut(s) 85, 782
HpyCH4III ACNGT 3 cut(s) 182, 667, 839
HpyCH4IV ACGT 1 cut(s) 424
HpyCH4V TGCA 6 cut(s) 386, 614, 650, 739, 758, 815
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 359, 761, 957
HpyF3I CTNAG 4 cut(s) 148, 197, 564, 690
HpySE526I ACGT 1 cut(s) 424
Hsp92II CATG 3 cut(s) 264, 658, 715
Kpn2I TCCGGA 1 cut(s) 41
Ksp22I TGATCA 2 cut(s) 601, 973
Kzo9I GATC 4 cut(s) 413, 601, 770, 973
LmnI GCTCC 3 cut(s) 44, 350, 935
Lsp1109I GCAGC 4 cut(s) 346, 742, 748, 770
LweI GCATC 5 cut(s) 241, 273, 447, 659, 834
MaeII ACGT 1 cut(s) 424
MalI GATC 4 cut(s) 415, 603, 772, 975
MboI GATC 4 cut(s) 413, 601, 770, 973
MboII GAAGA 3 cut(s) 413, 791, 997
MflI RGATCY 1 cut(s) 770
MhlI GDGCHC 2 cut(s) 32, 355
MluCI AATT 8 cut(s) 78, 95, 104, 474, 546, 734, 850, 969
MlyI GAGTC 2 cut(s) 413, 474
MmeI TCCRAC 2 cut(s) 90, 515
MnlI CCTC 8 cut(s) 194, 208, 385, 461, 474, 514, 573, 963
Mph1103I ATGCAT 2 cut(s) 652, 817
MroI TCCGGA 1 cut(s) 41
MroXI GAANNNNTTC 1 cut(s) 989
MseI TTAA 3 cut(s) 63, 81, 1000
MslI CAYNNNNRTG 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 761
MspI CCGG 5 cut(s) 19, 42, 380, 495, 961
MspR9I CCNGG 1 cut(s) 310
Mva1269I GAATGC 1 cut(s) 694
MvaI CCWGG 1 cut(s) 310
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 4 cut(s) 44, 359, 761, 957
NcoI CCATGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 909
NdeII GATC 4 cut(s) 413, 601, 770, 973
NlaIII CATG 3 cut(s) 264, 658, 715
NlaIV GGNNCC 1 cut(s) 17
NsiI ATGCAT 2 cut(s) 652, 817
PaeR7I CTCGAG 1 cut(s) 354
PceI AGGCCT 1 cut(s) 450
PcsI WCGNNNNNNNCGW 1 cut(s) 556
PctI GAATGC 1 cut(s) 694
PdmI GAANNNNTTC 1 cut(s) 989
PfeI GAWTC 4 cut(s) 58, 113, 272, 553
PinAI ACCGGT 1 cut(s) 494
PkrI GCNGC 5 cut(s) 361, 726, 757, 760, 763
PleI GAGTC 2 cut(s) 412, 473
PpsI GAGTC 2 cut(s) 412, 473
Ppu21I YACGTR 1 cut(s) 425
PshBI ATTAAT 1 cut(s) 81
Psp124BI GAGCTC 2 cut(s) 32, 355
Psp6I CCWGG 1 cut(s) 308
PspGI CCWGG 1 cut(s) 308
PspN4I GGNNCC 1 cut(s) 17
PspPI GGNCC 2 cut(s) 16, 572
PspXI VCTCGAGB 1 cut(s) 354
PstI CTGCAG 1 cut(s) 760
PsuI RGATCY 1 cut(s) 770
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 8 cut(s) 314, 423, 486, 499, 544, 641, 881, 922
RsaNI GTAC 8 cut(s) 313, 422, 485, 498, 543, 640, 880, 921
RseI CAYNNNNRTG 1 cut(s) 249
SacI GAGCTC 2 cut(s) 32, 355
SaqAI TTAA 3 cut(s) 63, 81, 1000
SatI GCNGC 5 cut(s) 360, 725, 756, 759, 762
Sau3AI GATC 4 cut(s) 413, 601, 770, 973
Sau96I GGNCC 2 cut(s) 16, 572
SchI GAGTC 2 cut(s) 413, 474
ScrFI CCNGG 1 cut(s) 310
SduI GDGCHC 2 cut(s) 32, 355
SfaNI GCATC 5 cut(s) 241, 273, 447, 659, 834
SfcI CTRYAG 1 cut(s) 756
Sfr274I CTCGAG 1 cut(s) 354
SinI GGWCC 1 cut(s) 16
SlaI CTCGAG 1 cut(s) 354
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 3 cut(s) 354, 452, 869
SmoI CTYRAG 3 cut(s) 354, 452, 869
Sse9I AATT 8 cut(s) 78, 95, 104, 474, 546, 734, 850, 969
SseBI AGGCCT 1 cut(s) 450
SsiI CCGC 4 cut(s) 143, 724, 887, 903
SstI GAGCTC 2 cut(s) 32, 355
StuI AGGCCT 1 cut(s) 450
StyD4I CCNGG 1 cut(s) 308
StyI CCWWGG 3 cut(s) 445, 619, 654
TaaI ACNGT 3 cut(s) 182, 667, 839
TaiI ACGT 1 cut(s) 427
TaqI TCGA 2 cut(s) 76, 355
TasI AATT 8 cut(s) 78, 95, 104, 474, 546, 734, 850, 969
TatI WGTACW 2 cut(s) 879, 920
TauI GCSGC 1 cut(s) 727
TfiI GAWTC 4 cut(s) 58, 113, 272, 553
Tru1I TTAA 3 cut(s) 63, 81, 1000
Tru9I TTAA 3 cut(s) 63, 81, 1000
TscAI CASTG 2 cut(s) 54, 196
TseI GCWGC 4 cut(s) 359, 755, 758, 761
TspGWI ACGGA 1 cut(s) 983
TspRI CASTG 2 cut(s) 54, 196
VpaK11BI GGWCC 1 cut(s) 16
VspI ATTAAT 1 cut(s) 81
XagI CCTNNNNNAGG 2 cut(s) 567, 872
XapI RAATTY 1 cut(s) 850
XhoI CTCGAG 1 cut(s) 354
XmnI GAANNNNTTC 1 cut(s) 989
Zsp2I ATGCAT 2 cut(s) 652, 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.