FvH4_2g25340

Protein LOW PSII ACCUMULATION 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011276
Physical Location & Seq
Forward (+)
20603037 .. 20606137
3101 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25340.t4

Sequence Viewer

Length: 1329 bp
ATGGCTGCTCAAGCTCTTCTGCACCAGCAATGGCTCTGCTTCTCCTCAAACCCGATATCAACCACAACCACAAGGCCATGGCTTCCCGCCAGAACCACCACCCTTTCCTTTTTCCAAATCCACAAGAAACTCATTCCCTGCTCTTCTTCTTCTTCAACTTCTCAGTCCCCTGAAGCCAATACAGAAACCGCCGAGTCCTGTGTCAACTTGGGCCTCCAGCTCTTCTCCAAAGGACGGGTTAAAGATGCTTTAGTTCAGTTCGAGACAGCACTCAATTTAGATCCCAATCCTATAGAGGCCCAAGCTGCTCTATATAATAAAGCTTGCTGTCACGCCTACAGAGGGGAAGGAAGCAAAGCTGCTGATTGCCTACGTACTTGCTTAAGAGAATATAATCTCAAATTTGGAACAATTTTGAATGATCCTGACTTGGCCTCTTTTCGAGCTTTGCCTGAATTTAAGGAATTGCAAGAAGAGGCCAGAATAGGTGGGGAAGACATAGGCTACAGTTTTAGGAGAGATCTGAAACTCATTAGTGAAGTTCAAGCACCATTCCGTGGGGTTAGGAGATTCTTTTATGTGGCATTCTCTGCAGCTGCAGGAATCTCTACATTCTTCACTGTACCTAGGCTGTTCCGTGCAATTTCAGGTGGTGATGGTGCTCCTGCTCTCTTGGAAACGGCTGGGAATGTTGCTATTAATGTTGGAGGTTTTACAGTTCTTGTGGCATTGTTTTTATGGGAAAACAAAAAGGAGGAGGAACAACTTGCACAAATATCTCGTGATGAAAGTCTGTCAAGGCTGCCATTACGGCTCTCCACCAATCGGGTTGTTGAACTTGTGCAGTTGCGGGACACTGTTAGACCTGTCATCCTAGCAGGGAAGAAGGAGACTGTTAATTCAAGTATGCAGCAGGCAGCGAGGTTCCGAACAGATCTTCTCTTAAGGGGAGTTCTATTGGTTCCTGTTATCTTTGGTGAAGTTAGGGCACCTAAAATAGAGAAGAAAGGTTTTGGCACTTCTTCAAAGGCAGCTGCCGCCCTGCCGTCTATTGGGGAAGATTTCGAGAAACGAACTCAGTCTATAACTGCAAAGTCAAAATTAAAAGCTGAAATTCGATTCAAGGCCGAGGTTGTATCCCCTGAGGAATGGGAAAGTTGGATAAGGGATCAGCAGAAGTCTGAAGGAGTCACTCCTGGTGAGGATGTCTACATCATACTGCGCCTGGATGGCCGGGTTCGAAGATCAGGGAAAGGTATGCCTGACTGGCAACAAATTCTGCAGGAGCTGCCTCCAGTTGAAGCATTGCTTAGCAAACTTGAAAGATAG

Protein Analysis

443

Amino Acids

49.07

Weight (kDa)

8.97

Isoelectric Point (pI)

52.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3493 PF11998 172 - 249 2.7e-24 Low psii accumulation1 / Rep27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02910 AT1G02910
fragaria_vesca FvH4_2g25340 FvH4_2g25340 FvH4_2g25340 FvH4_2g25340
malus_domestica MD03G1284800.v1.1 MD11G1304300.v1.1
prunus_persica Prupe.8G257900_v2.0.a1
pyrus_communis pycom11g26620
rosa_chinensis RchiOBHm_Chr6g0293521
rosa_laevigata RLG00000011944
rosa_multiflora Rmu_sc0006269.1_g000009
rosa_roxburghii Rroxscaffold_7G00174120
rosa_rugosa Rorug06G0236000 Rorug06G0236100.1
rosa_samantha Rh6AG348800 Rh6CG362900 Rh6DG349800
rosa_wichuraiana Rw6G030350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 988
AccB7I CCANNNNNTGG 1 cut(s) 557
AccI GTMKAC 1 cut(s) 1209
AciI CCGC 4 cut(s) 87, 189, 850, 1038
AclWI GGATC 3 cut(s) 275, 416, 1176
AcoI YGGCCR 1 cut(s) 1231
AcsI RAATTY 4 cut(s) 401, 455, 1113, 1275
AcuI CTGAAG 2 cut(s) 192, 1203
AfaI GTAC 2 cut(s) 376, 624
AfiI CCNNNNNNNGG 5 cut(s) 234, 342, 557, 825, 1052
AflII CTTAAG 2 cut(s) 382, 943
AgsI TTSAA 9 cut(s) 156, 418, 545, 836, 903, 1026, 1123, 1301, 1322
AjnI CCWGG 2 cut(s) 1195, 1224
Alw21I GWGCWC 1 cut(s) 664
Alw26I GTCTC 2 cut(s) 257, 884
AlwI GGATC 3 cut(s) 275, 416, 1176
AlwNI CAGNNNCTG 1 cut(s) 1288
AoxI GGCC 7 cut(s) 74, 211, 297, 432, 477, 1125, 1231
ApoI RAATTY 4 cut(s) 401, 455, 1113, 1275
AseI ATTAAT 1 cut(s) 699
AspA2I CCTAGG 1 cut(s) 626
AspLEI GCGC 1 cut(s) 1224
AspS9I GGNCC 2 cut(s) 211, 298
AsuC2I CCSGG 1 cut(s) 1235
AsuHPI GGTGA 3 cut(s) 665, 989, 1211
AsuII TTCGAA 1 cut(s) 1240
AvrII CCTAGG 1 cut(s) 626
AxyI CCTNAGG 1 cut(s) 1143
BaeGI GKGCMC 1 cut(s) 991
BanI GGYRCC 1 cut(s) 988
BauI CACGAG 1 cut(s) 780
BbsI GAAGAC 1 cut(s) 501
Bbv12I GWGCWC 1 cut(s) 664
BccI CCATC 2 cut(s) 650, 1223
BceAI ACGGC 3 cut(s) 696, 827, 1030
BciT130I CCWGG 2 cut(s) 1197, 1226
BciVI GTATCC 1 cut(s) 1147
BcnI CCSGG 1 cut(s) 1235
BcoDI GTCTC 2 cut(s) 257, 884
BfaI CTAG 2 cut(s) 627, 875
BfmI CTRYAG 6 cut(s) 291, 337, 505, 591, 597, 1280
BfrI CTTAAG 2 cut(s) 382, 943
BfuI GTATCC 1 cut(s) 1147
BglI GCCNNNNNGGC 3 cut(s) 811, 1230, 1267
BglII AGATCT 2 cut(s) 520, 934
BlnI CCTAGG 1 cut(s) 626
BlpI GCTNAGC 1 cut(s) 1310
Bme1390I CCNGG 3 cut(s) 1197, 1226, 1235
BmgT120I GGNCC 2 cut(s) 211, 298
BmiI GGNNCC 3 cut(s) 926, 963, 990
BmrFI CCNGG 3 cut(s) 1197, 1226, 1235
BmsI GCATC 1 cut(s) 235
BpiI GAAGAC 1 cut(s) 501
BpmI CTGGAG 2 cut(s) 200, 1278
Bpu1102I GCTNAGC 1 cut(s) 1310
Bpu14I TTCGAA 1 cut(s) 1240
BpuMI CCSGG 1 cut(s) 1235
BsaAI YACGTR 1 cut(s) 374
BsaBI GATNNNNATC 1 cut(s) 285
BsaJI CCNNGG 4 cut(s) 77, 556, 626, 1128
Bsc4I CCNNNNNNNGG 5 cut(s) 234, 342, 557, 825, 1052
Bse1I ACTGG 2 cut(s) 1271, 1295
Bse21I CCTNAGG 1 cut(s) 1143
Bse3DI GCAATG 2 cut(s) 35, 1304
Bse8I GATNNNNATC 1 cut(s) 285
BseBI CCWGG 2 cut(s) 1197, 1226
BseDI CCNNGG 4 cut(s) 77, 556, 626, 1128
BseGI GGATG 3 cut(s) 870, 1210, 1234
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 5 cut(s) 234, 342, 557, 825, 1052
BseMI GCAATG 2 cut(s) 35, 1304
BseMII CTCAG 3 cut(s) 176, 1091, 1134
BseNI ACTGG 2 cut(s) 1271, 1295
BseRI GAGGAG 2 cut(s) 34, 770
BseSI GKGCMC 1 cut(s) 991
BseYI CCCAGC 1 cut(s) 683
BsgI GTGCAG 2 cut(s) 5, 863
BshFI GGCC 7 cut(s) 76, 213, 299, 434, 479, 1127, 1233
BshNI GGYRCC 1 cut(s) 988
BsiHKAI GWGCWC 1 cut(s) 664
BsiSI CCGG 1 cut(s) 1234
BslFI GGGAC 2 cut(s) 151, 866
BslI CCNNNNNNNGG 5 cut(s) 234, 342, 557, 825, 1052
BsmAI GTCTC 2 cut(s) 257, 884
BsmFI GGGAC 2 cut(s) 151, 866
BsmI GAATGC 1 cut(s) 584
BsnI GGCC 7 cut(s) 76, 213, 299, 434, 479, 1127, 1233
Bsp119I TTCGAA 1 cut(s) 1240
Bsp1286I GDGCHC 2 cut(s) 664, 991
Bsp143I GATC 6 cut(s) 280, 421, 520, 934, 1168, 1244
Bsp1720I GCTNAGC 1 cut(s) 1310
Bsp19I CCATGG 1 cut(s) 77
BspACI CCGC 4 cut(s) 87, 189, 850, 1038
BspANI GGCC 7 cut(s) 76, 213, 299, 434, 479, 1127, 1233
BspCNI CTCAG 3 cut(s) 175, 1090, 1135
BspLI GGNNCC 3 cut(s) 926, 963, 990
BspMAI CTGCAG 3 cut(s) 595, 601, 1284
BspPI GGATC 3 cut(s) 275, 416, 1176
BspQI GCTCTTC 3 cut(s) 21, 148, 227
BspT104I TTCGAA 1 cut(s) 1240
BspT107I GGYRCC 1 cut(s) 988
BspTI CTTAAG 2 cut(s) 382, 943
BsrDI GCAATG 2 cut(s) 35, 1304
BsrI ACTGG 2 cut(s) 1271, 1295
BssECI CCNNGG 4 cut(s) 77, 556, 626, 1128
BssMI GATC 6 cut(s) 280, 421, 520, 934, 1168, 1244
BssSI CACGAG 1 cut(s) 780
BssT1I CCWWGG 2 cut(s) 77, 626
Bst2BI CACGAG 1 cut(s) 780
Bst2UI CCWGG 2 cut(s) 1197, 1226
Bst4CI ACNGT 5 cut(s) 509, 622, 718, 859, 895
Bst6I CTCTTC 4 cut(s) 21, 148, 227, 468
BstAFI CTTAAG 2 cut(s) 382, 943
BstAPI GCANNNNNTGC 2 cut(s) 590, 1288
BstBAI YACGTR 1 cut(s) 374
BstBI TTCGAA 1 cut(s) 1240
BstC8I GCNNGC 2 cut(s) 325, 915
BstDEI CTNAG 4 cut(s) 162, 1077, 1143, 1310
BstDSI CCRYGG 2 cut(s) 77, 556
BstF5I GGATG 3 cut(s) 870, 1210, 1234
BstHHI GCGC 1 cut(s) 1224
BstKTI GATC 6 cut(s) 283, 424, 523, 937, 1171, 1247
BstMAI GTCTC 2 cut(s) 257, 884
BstMBI GATC 6 cut(s) 280, 421, 520, 934, 1168, 1244
BstMWI GCNNNNNNNGC 8 cut(s) 11, 305, 590, 811, 1037, 1230, 1267, 1288
BstNI CCWGG 2 cut(s) 1197, 1226
BstSCI CCNGG 3 cut(s) 1195, 1224, 1233
BstSFI CTRYAG 6 cut(s) 291, 337, 505, 591, 597, 1280
BstSLI GKGCMC 1 cut(s) 991
BstSNI TACGTA 1 cut(s) 374
BstV2I GAAGAC 1 cut(s) 501
BstX2I RGATCY 3 cut(s) 280, 520, 934
BstYI RGATCY 3 cut(s) 280, 520, 934
Bsu36I CCTNAGG 1 cut(s) 1143
BsuI GTATCC 1 cut(s) 1147
BsuRI GGCC 7 cut(s) 76, 213, 299, 434, 479, 1127, 1233
BtgI CCRYGG 2 cut(s) 77, 556
BtsCI GGATG 3 cut(s) 870, 1210, 1234
BtsIMutI CAGTG 2 cut(s) 618, 855
Cac8I GCNNGC 2 cut(s) 325, 915
CaiI CAGNNNCTG 1 cut(s) 1288
CfoI GCGC 1 cut(s) 1224
Cfr13I GGNCC 2 cut(s) 211, 298
Csp6I GTAC 2 cut(s) 375, 623
CviAII CATG 1 cut(s) 78
CviQI GTAC 2 cut(s) 375, 623
DdeI CTNAG 4 cut(s) 162, 1077, 1143, 1310
DpnI GATC 6 cut(s) 282, 423, 522, 936, 1170, 1246
DpnII GATC 6 cut(s) 280, 421, 520, 934, 1168, 1244
EaeI YGGCCR 1 cut(s) 1231
Eam1104I CTCTTC 4 cut(s) 21, 148, 227, 468
EarI CTCTTC 4 cut(s) 21, 148, 227, 468
Eco105I TACGTA 1 cut(s) 374
Eco130I CCWWGG 2 cut(s) 77, 626
Eco32I GATATC 1 cut(s) 57
Eco57I CTGAAG 2 cut(s) 192, 1203
Eco81I CCTNAGG 1 cut(s) 1143
EcoRII CCWGG 2 cut(s) 1195, 1224
EcoRV GATATC 1 cut(s) 57
EcoT14I CCWWGG 2 cut(s) 77, 626
ErhI CCWWGG 2 cut(s) 77, 626
FaeI CATG 1 cut(s) 81
FalI AAGNNNNNCTT 2 cut(s) 1293, 1325
FaqI GGGAC 2 cut(s) 151, 866
FatI CATG 1 cut(s) 77
FauI CCCGC 2 cut(s) 94, 843
FblI GTMKAC 1 cut(s) 1209
FokI GGATG 3 cut(s) 857, 1217, 1241
FspBI CTAG 2 cut(s) 627, 875
GlaI GCGC 1 cut(s) 1223
GsaI CCCAGC 1 cut(s) 687
GsuI CTGGAG 2 cut(s) 200, 1278
HaeIII GGCC 7 cut(s) 76, 213, 299, 434, 479, 1127, 1233
HapII CCGG 1 cut(s) 1234
HhaI GCGC 1 cut(s) 1224
Hin1II CATG 1 cut(s) 81
Hin6I GCGC 1 cut(s) 1222
HinP1I GCGC 1 cut(s) 1222
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 321
HinfI GANTC 5 cut(s) 194, 570, 603, 1119, 1188
HpaII CCGG 1 cut(s) 1234
HphI GGTGA 3 cut(s) 665, 989, 1211
Hpy166II GTNNAC 2 cut(s) 205, 1210
Hpy188I TCNGA 3 cut(s) 525, 929, 1183
Hpy188III TCNNGA 4 cut(s) 262, 425, 782, 1066
Hpy8I GTNNAC 2 cut(s) 205, 1210
HpyAV CCTTC 3 cut(s) 341, 880, 1178
HpyCH4III ACNGT 5 cut(s) 509, 622, 718, 859, 895
HpyCH4IV ACGT 1 cut(s) 373
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 305, 590, 811, 1037, 1230, 1267, 1288
HpyF3I CTNAG 4 cut(s) 162, 1077, 1143, 1310
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 1 cut(s) 81
HspAI GCGC 1 cut(s) 1222
Kzo9I GATC 6 cut(s) 280, 421, 520, 934, 1168, 1244
LguI GCTCTTC 3 cut(s) 21, 148, 227
LmnI GCTCC 2 cut(s) 667, 1285
LweI GCATC 1 cut(s) 235
MaeI CTAG 2 cut(s) 627, 875
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 2 cut(s) 329, 1189
MalI GATC 6 cut(s) 282, 423, 522, 936, 1170, 1246
MboI GATC 6 cut(s) 280, 421, 520, 934, 1168, 1244
MflI RGATCY 3 cut(s) 280, 520, 934
MhlI GDGCHC 2 cut(s) 664, 991
MlyI GAGTC 2 cut(s) 203, 1197
MmeI TCCRAC 2 cut(s) 685, 1139
MseI TTAA 7 cut(s) 240, 383, 459, 699, 897, 944, 1103
MspA1I CMGCKG 2 cut(s) 596, 1034
MspCI CTTAAG 2 cut(s) 382, 943
MspI CCGG 1 cut(s) 1234
MspR9I CCNGG 3 cut(s) 1197, 1226, 1235
Mva1269I GAATGC 1 cut(s) 584
MvaI CCWGG 2 cut(s) 1197, 1226
MwoI GCNNNNNNNGC 8 cut(s) 11, 305, 590, 811, 1037, 1230, 1267, 1288
NciI CCSGG 1 cut(s) 1235
NcoI CCATGG 1 cut(s) 77
NdeII GATC 6 cut(s) 280, 421, 520, 934, 1168, 1244
NlaIII CATG 1 cut(s) 81
NlaIV GGNNCC 3 cut(s) 926, 963, 990
NmeAIII GCCGAG 2 cut(s) 217, 1153
NmuCI GTSAC 2 cut(s) 329, 1189
NspV TTCGAA 1 cut(s) 1240
PciSI GCTCTTC 3 cut(s) 21, 148, 227
PctI GAATGC 1 cut(s) 584
PfeI GAWTC 3 cut(s) 570, 603, 1119
PflMI CCANNNNNTGG 1 cut(s) 557
PleI GAGTC 2 cut(s) 202, 1196
PpsI GAGTC 2 cut(s) 202, 1196
Ppu21I YACGTR 1 cut(s) 374
PshBI ATTAAT 1 cut(s) 699
Psp6I CCWGG 2 cut(s) 1195, 1224
PspFI CCCAGC 1 cut(s) 683
PspGI CCWGG 2 cut(s) 1195, 1224
PspN4I GGNNCC 3 cut(s) 926, 963, 990
PspPI GGNCC 2 cut(s) 211, 298
PstI CTGCAG 3 cut(s) 595, 601, 1284
PstNI CAGNNNCTG 1 cut(s) 1288
PsuI RGATCY 3 cut(s) 280, 520, 934
PvuII CAGCTG 2 cut(s) 596, 1034
RsaI GTAC 2 cut(s) 376, 624
RsaNI GTAC 2 cut(s) 375, 623
SapI GCTCTTC 3 cut(s) 21, 148, 227
SaqAI TTAA 7 cut(s) 240, 383, 459, 699, 897, 944, 1103
Sau3AI GATC 6 cut(s) 280, 421, 520, 934, 1168, 1244
Sau96I GGNCC 2 cut(s) 211, 298
SchI GAGTC 2 cut(s) 203, 1197
ScrFI CCNGG 3 cut(s) 1197, 1226, 1235
SduI GDGCHC 2 cut(s) 664, 991
SfaNI GCATC 1 cut(s) 235
SfcI CTRYAG 6 cut(s) 291, 337, 505, 591, 597, 1280
SfuI TTCGAA 1 cut(s) 1240
SmlI CTYRAG 3 cut(s) 9, 382, 943
SmoI CTYRAG 3 cut(s) 9, 382, 943
SnaBI TACGTA 1 cut(s) 374
SsiI CCGC 4 cut(s) 87, 189, 850, 1038
SspMI CTAG 2 cut(s) 627, 875
StyD4I CCNGG 3 cut(s) 1195, 1224, 1233
StyI CCWWGG 2 cut(s) 77, 626
TaaI ACNGT 5 cut(s) 509, 622, 718, 859, 895
TaiI ACGT 1 cut(s) 376
TaqI TCGA 5 cut(s) 261, 442, 1065, 1117, 1240
TauI GCSGC 1 cut(s) 1040
TfiI GAWTC 3 cut(s) 570, 603, 1119
Tru1I TTAA 7 cut(s) 240, 383, 459, 699, 897, 944, 1103
Tru9I TTAA 7 cut(s) 240, 383, 459, 699, 897, 944, 1103
TscAI CASTG 2 cut(s) 625, 862
TseFI GTSAC 2 cut(s) 329, 1189
Tsp45I GTSAC 2 cut(s) 329, 1189
TspDTI ATGAA 1 cut(s) 801
TspGWI ACGGA 2 cut(s) 545, 626
TspRI CASTG 2 cut(s) 625, 862
Van91I CCANNNNNTGG 1 cut(s) 557
Vha464I CTTAAG 2 cut(s) 382, 943
VspI ATTAAT 1 cut(s) 699
XapI RAATTY 4 cut(s) 401, 455, 1113, 1275
XmaJI CCTAGG 1 cut(s) 626
XmiI GTMKAC 1 cut(s) 1209
XspI CTAG 2 cut(s) 627, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.