FvH4_2g25700

Peter Pan-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008352
Physical Location & Seq
Reverse (-)
20769430 .. 20772204
2775 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25700.t2

Sequence Viewer

Length: 1041 bp
ATGGCTCGTTTTAATAATAAGAGGAAGCCATTTGTGAAACCAATTGCAAAGAAGAAGCTGGCTACTGTAGACCATATCACAGGTGATAAGATCCCAAAGAGCTTTGTGTTTTCAAGAGGGAGGTTGCCTGGTCCTCTTAGGCAACTCAAAGCTGACTTGAAACAGTTGATGAGTCCCCATACTGCTCTCAATCTTAAGGAGAAGAGACGGAACACTCTCAAGGACTTTCTGAATGTTGCTGGGCCTTATGGGGTTACACATTTCCTCATATTGTCGAAAACACTAACTGCACCCTACCTTAGAGTTGCCACAACTCCTCAAGGGCCAACTCTTACATTTAAAATAGAAGAGTACTCACTGGCAGTTGACATCAAACATTCTCAGAAGTTTCCAAGATGTCCGAAAGATCTTTTCAAGAATCCACCCATGAGTATTCTTTCTGGTTTTGGAAGTGGTGCTGGTCGCCACCAAGAACCTCCACAGTTAAAGCTCATATCTGCAGCATTTCGGAATATCTTTCCATCTATTGATGTTAACACTGTGAAACTTTCTTCCTGCCAGAGAATATTGTTGCTTCATTACAATGAAGACACAAAACTAATTGATTTTCGGCATTACTCTATAAGATTAGCGCCTGTTGGTGTTTCCCGCAGAATAAGAAAATTTGTTCAGAAGCATGAAGTACCTGATTTGAGGAATCTTCAAGACGTGAGTGACTTTGTCACAAAGGCTGGGTATGGATCAGAAAGTGAAGGAGATGAAGAAGCAGCAACGGTAACTTTGGCTAGTGATCTAGGTAGGGTTAATCGGGCTTCTTCAAAAAGTGCTATTAAGCTCCAAGAGATAGGACCCAGGATGACTCTTCGCCTGATTAAGGTTGAGAATGGATTGTGTCAAGGCGCAGTCCTTTTCAGTGAGTATGGTGATGGAAAGAAAAATGAAGACAACCACGAAGATGCTGAAAATGAGGAAGATGGCGATGAGGAGGAAGATGATGAAAATGAGGAAACTGATAGTGAGGACGAGGAAGAAGATGACTAG

Protein Analysis

347

Amino Acids

38.9

Weight (kDa)

6.78

Isoelectric Point (pI)

50.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 29 - 255 3.3e-35 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 69
AciI CCGC 1 cut(s) 649
AclWI GGATC 2 cut(s) 85, 748
AcsI RAATTY 1 cut(s) 662
AfaI GTAC 2 cut(s) 353, 684
AfiI CCNNNNNNNGG 1 cut(s) 874
AflII CTTAAG 1 cut(s) 194
AgsI TTSAA 5 cut(s) 114, 160, 415, 704, 819
AjiI CACGTC 1 cut(s) 709
AjnI CCWGG 2 cut(s) 127, 851
AluBI AGCT 5 cut(s) 58, 102, 152, 490, 835
AluI AGCT 5 cut(s) 58, 102, 152, 490, 835
Alw26I GTCTC 1 cut(s) 199
AlwI GGATC 2 cut(s) 85, 748
AoxI GGCC 2 cut(s) 242, 323
ApeKI GCWGC 2 cut(s) 500, 767
ApoI RAATTY 1 cut(s) 662
AspLEI GCGC 2 cut(s) 634, 902
AspS9I GGNCC 4 cut(s) 131, 242, 323, 848
AsuHPI GGTGA 2 cut(s) 95, 935
AvaII GGWCC 2 cut(s) 131, 848
BbsI GAAGAC 2 cut(s) 594, 948
BbvI GCAGC 2 cut(s) 512, 779
BccI CCATC 3 cut(s) 529, 920, 968
BciT130I CCWGG 2 cut(s) 129, 853
BcoDI GTCTC 1 cut(s) 199
BfaI CTAG 3 cut(s) 786, 794, 1039
BfmI CTRYAG 2 cut(s) 66, 498
BfoI RGCGCY 1 cut(s) 635
BfrI CTTAAG 1 cut(s) 194
BglII AGATCT 1 cut(s) 406
BisI GCNGC 2 cut(s) 501, 768
BlsI GCNGC 2 cut(s) 502, 769
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 2 cut(s) 129, 853
Bme18I GGWCC 2 cut(s) 131, 848
BmgBI CACGTC 1 cut(s) 709
BmgT120I GGNCC 4 cut(s) 131, 242, 323, 848
BmiI GGNNCC 1 cut(s) 850
BmrFI CCNGG 2 cut(s) 129, 853
BmsI GCATC 1 cut(s) 946
BpiI GAAGAC 2 cut(s) 594, 948
BpuEI CTTGAG 2 cut(s) 203, 303
BsaJI CCNNGG 1 cut(s) 851
Bsc4I CCNNNNNNNGG 1 cut(s) 874
Bse1I ACTGG 1 cut(s) 363
BseBI CCWGG 2 cut(s) 129, 853
BseDI CCNNGG 1 cut(s) 851
BseGI GGATG 1 cut(s) 861
BseLI CCNNNNNNNGG 1 cut(s) 874
BseMII CTCAG 1 cut(s) 395
BseNI ACTGG 1 cut(s) 363
BseRI GAGGAG 2 cut(s) 306, 998
BseXI GCAGC 2 cut(s) 512, 779
BseYI CCCAGC 2 cut(s) 239, 731
BsgI GTGCAG 1 cut(s) 273
BshFI GGCC 2 cut(s) 244, 325
BslFI GGGAC 1 cut(s) 159
BslI CCNNNNNNNGG 1 cut(s) 874
BsmAI GTCTC 1 cut(s) 199
BsmBI CGTCTC 1 cut(s) 199
BsmFI GGGAC 1 cut(s) 159
BsnI GGCC 2 cut(s) 244, 325
Bsp143I GATC 4 cut(s) 90, 406, 740, 790
BspACI CCGC 1 cut(s) 649
BspANI GGCC 2 cut(s) 244, 325
BspCNI CTCAG 1 cut(s) 394
BspLI GGNNCC 1 cut(s) 850
BspMAI CTGCAG 1 cut(s) 502
BspPI GGATC 2 cut(s) 85, 748
BspTI CTTAAG 1 cut(s) 194
BsrI ACTGG 1 cut(s) 363
BssECI CCNNGG 1 cut(s) 851
BssMI GATC 4 cut(s) 90, 406, 740, 790
Bst2UI CCWGG 2 cut(s) 129, 853
Bst4CI ACNGT 5 cut(s) 67, 165, 483, 541, 775
Bst6I CTCTTC 3 cut(s) 197, 342, 867
BstAFI CTTAAG 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 3 cut(s) 137, 299, 381
BstENI CCTNNNNNAGG 1 cut(s) 872
BstF5I GGATG 1 cut(s) 861
BstH2I RGCGCY 1 cut(s) 635
BstHHI GCGC 2 cut(s) 634, 902
BstKTI GATC 4 cut(s) 93, 409, 743, 793
BstMAI GTCTC 1 cut(s) 199
BstMBI GATC 4 cut(s) 90, 406, 740, 790
BstNI CCWGG 2 cut(s) 129, 853
BstSCI CCNGG 2 cut(s) 127, 851
BstSFI CTRYAG 2 cut(s) 66, 498
BstV1I GCAGC 2 cut(s) 512, 779
BstV2I GAAGAC 2 cut(s) 594, 948
BstX2I RGATCY 2 cut(s) 90, 406
BstYI RGATCY 2 cut(s) 90, 406
BsuRI GGCC 2 cut(s) 244, 325
BtgZI GCGATG 1 cut(s) 993
BtrI CACGTC 1 cut(s) 709
BtsCI GGATG 1 cut(s) 861
BtsIMutI CAGTG 3 cut(s) 356, 537, 919
Cac8I GCNNGC 1 cut(s) 60
CfoI GCGC 2 cut(s) 634, 902
Cfr13I GGNCC 4 cut(s) 131, 242, 323, 848
Csp6I GTAC 2 cut(s) 352, 683
CviAII CATG 2 cut(s) 427, 677
CviQI GTAC 2 cut(s) 352, 683
DdeI CTNAG 3 cut(s) 137, 299, 381
DpnI GATC 4 cut(s) 92, 408, 742, 792
DpnII GATC 4 cut(s) 90, 406, 740, 790
DraI TTTAAA 1 cut(s) 340
Eam1104I CTCTTC 3 cut(s) 197, 342, 867
EarI CTCTTC 3 cut(s) 197, 342, 867
Eco47I GGWCC 2 cut(s) 131, 848
EcoNI CCTNNNNNAGG 1 cut(s) 872
EcoO109I RGGNCCY 1 cut(s) 848
EcoRII CCWGG 2 cut(s) 127, 851
Esp3I CGTCTC 1 cut(s) 199
FaeI CATG 2 cut(s) 430, 680
FaqI GGGAC 1 cut(s) 159
FatI CATG 2 cut(s) 426, 676
FauI CCCGC 1 cut(s) 656
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 2 cut(s) 501, 768
FokI GGATG 1 cut(s) 868
Fsp4HI GCNGC 2 cut(s) 501, 768
FspBI CTAG 3 cut(s) 786, 794, 1039
GlaI GCGC 2 cut(s) 633, 901
GluI GCNGC 2 cut(s) 501, 768
GsaI CCCAGC 2 cut(s) 243, 735
HaeII RGCGCY 1 cut(s) 635
HaeIII GGCC 2 cut(s) 244, 325
HhaI GCGC 2 cut(s) 634, 902
Hin1II CATG 2 cut(s) 430, 680
Hin6I GCGC 2 cut(s) 632, 900
HinP1I GCGC 2 cut(s) 632, 900
HincII GTYRAC 2 cut(s) 367, 535
HindII GTYRAC 2 cut(s) 367, 535
HinfI GANTC 4 cut(s) 172, 418, 697, 859
HpaI GTTAAC 1 cut(s) 535
HphI GGTGA 2 cut(s) 95, 935
Hpy166II GTNNAC 3 cut(s) 70, 367, 535
Hpy188I TCNGA 6 cut(s) 231, 384, 402, 510, 672, 745
Hpy188III TCNNGA 3 cut(s) 114, 415, 704
Hpy8I GTNNAC 3 cut(s) 70, 367, 535
HpyAV CCTTC 1 cut(s) 746
HpyCH4III ACNGT 5 cut(s) 67, 165, 483, 541, 775
HpyCH4IV ACGT 1 cut(s) 708
HpyCH4V TGCA 3 cut(s) 47, 290, 500
HpyF3I CTNAG 3 cut(s) 137, 299, 381
HpySE526I ACGT 1 cut(s) 708
Hsp92II CATG 2 cut(s) 430, 680
HspAI GCGC 2 cut(s) 632, 900
KspAI GTTAAC 1 cut(s) 535
Kzo9I GATC 4 cut(s) 90, 406, 740, 790
LmnI GCTCC 1 cut(s) 840
Lsp1109I GCAGC 2 cut(s) 512, 779
LweI GCATC 1 cut(s) 946
MaeI CTAG 3 cut(s) 786, 794, 1039
MaeII ACGT 1 cut(s) 708
MaeIII GTNAC 4 cut(s) 253, 713, 721, 775
MalI GATC 4 cut(s) 92, 408, 742, 792
MboI GATC 4 cut(s) 90, 406, 740, 790
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 2 cut(s) 90, 406
MluCI AATT 3 cut(s) 42, 600, 662
MlyI GAGTC 2 cut(s) 181, 853
MseI TTAA 8 cut(s) 12, 195, 339, 485, 534, 804, 831, 873
MslI CAYNNNNRTG 2 cut(s) 582, 954
MspCI CTTAAG 1 cut(s) 194
MspR9I CCNGG 2 cut(s) 129, 853
MunI CAATTG 1 cut(s) 42
MvaI CCWGG 2 cut(s) 129, 853
NdeII GATC 4 cut(s) 90, 406, 740, 790
NlaIII CATG 2 cut(s) 430, 680
NlaIV GGNNCC 1 cut(s) 850
NmuCI GTSAC 2 cut(s) 713, 721
PfeI GAWTC 2 cut(s) 418, 697
PflFI GACNNNGTC 1 cut(s) 719
PkrI GCNGC 2 cut(s) 502, 769
PleI GAGTC 2 cut(s) 180, 853
PpsI GAGTC 2 cut(s) 180, 853
PpuMI RGGWCCY 1 cut(s) 848
Psp5II RGGWCCY 1 cut(s) 848
Psp6I CCWGG 2 cut(s) 127, 851
PspFI CCCAGC 2 cut(s) 239, 731
PspGI CCWGG 2 cut(s) 127, 851
PspN4I GGNNCC 1 cut(s) 850
PspPI GGNCC 4 cut(s) 131, 242, 323, 848
PspPPI RGGWCCY 1 cut(s) 848
PstI CTGCAG 1 cut(s) 502
PsuI RGATCY 2 cut(s) 90, 406
PsyI GACNNNGTC 1 cut(s) 719
RsaI GTAC 2 cut(s) 353, 684
RsaNI GTAC 2 cut(s) 352, 683
RseI CAYNNNNRTG 2 cut(s) 582, 954
SaqAI TTAA 8 cut(s) 12, 195, 339, 485, 534, 804, 831, 873
SatI GCNGC 2 cut(s) 501, 768
Sau3AI GATC 4 cut(s) 90, 406, 740, 790
Sau96I GGNCC 4 cut(s) 131, 242, 323, 848
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 2 cut(s) 181, 853
ScrFI CCNGG 2 cut(s) 129, 853
SfaNI GCATC 1 cut(s) 946
SfcI CTRYAG 2 cut(s) 66, 498
SinI GGWCC 2 cut(s) 131, 848
SmiMI CAYNNNNRTG 2 cut(s) 582, 954
SmlI CTYRAG 3 cut(s) 194, 218, 318
SmoI CTYRAG 3 cut(s) 194, 218, 318
Sse9I AATT 3 cut(s) 42, 600, 662
SsiI CCGC 1 cut(s) 649
SspI AATATT 1 cut(s) 567
SspMI CTAG 3 cut(s) 786, 794, 1039
StyD4I CCNGG 2 cut(s) 127, 851
TaaI ACNGT 5 cut(s) 67, 165, 483, 541, 775
TaiI ACGT 1 cut(s) 711
TaqI TCGA 1 cut(s) 275
TasI AATT 3 cut(s) 42, 600, 662
TatI WGTACW 1 cut(s) 351
TfiI GAWTC 2 cut(s) 418, 697
Tru1I TTAA 8 cut(s) 12, 195, 339, 485, 534, 804, 831, 873
Tru9I TTAA 8 cut(s) 12, 195, 339, 485, 534, 804, 831, 873
TscAI CASTG 3 cut(s) 363, 544, 919
TseFI GTSAC 2 cut(s) 713, 721
TseI GCWGC 2 cut(s) 500, 767
Tsp45I GTSAC 2 cut(s) 713, 721
TspDTI ATGAA 6 cut(s) 566, 600, 693, 774, 954, 1011
TspGWI ACGGA 1 cut(s) 223
TspRI CASTG 3 cut(s) 363, 544, 919
Tth111I GACNNNGTC 1 cut(s) 719
Vha464I CTTAAG 1 cut(s) 194
VpaK11BI GGWCC 2 cut(s) 131, 848
XagI CCTNNNNNAGG 1 cut(s) 872
XapI RAATTY 1 cut(s) 662
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 3 cut(s) 786, 794, 1039
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.