MD11G1310300.v1.1

Peter Pan-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008352
Physical Location & Seq
Forward (+)
42341024 .. 42343568
2545 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1310300.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGCTCGCTTCAAAAATAAGAAGAAGGTGTTTGTGAAACCGGTTTCGTCGAAGAAGCAGGCTAATGTGGACCATGTCACTGGGGACAAAATCCCAAGGAGCTTTGTGTTTTCCAGATGCAAGTTGCCTGGTCCTCTGAGGCAACTTCAAGCTGATTTGAGGAAGCTGATGCTTCCCTACACTGCCCTTAAGCTTAAGGAGAAGAGGCGGAATAATCTTAGAGACTTTTTGAATGTGGCCGGGCCAATGGGGGTTACACACTTCCTCATGTTGTCGAAAACTCCAACTGCTCCGTACCTTAGGGTTGCGAGGACGCCTCAAGGCCCAACGCTTACATTTAAGATACAGGAGTACTCTTTGGCAGTAGATATTGCGCGGTCTCAGAAGCGTCCTCGATGCCCAGCAGATCTTTTTAAGAATGCCCCTTTGATTGTTCTTTCTGGTTTTGGGACTGGAGAGCAACATTTGAAGCTCACAACCATACTGTTTCAGAACATCTTTCCAGCCATTGATGTTAACACTGTGAAACTTTCTTCATGCCAAAGAATAGTGTTGCTTAATTACAACAAAGACACAAAACTGATTGATTTTCGGCATTACTCCATAAGATTACAGCCTGTGGGTGTCTCCCAGAGATTAAGAAGATTTGTTCAGAACCATCAAGTCCCTGATCTGAGAAATCTTCAAGACGTGAGCGACTTTGTCACGAAGGCTGGTTACGGATCAGAAAGTGAAGCAGATGATGAGGCAGCGACCGTGACTTTGGCTAATGATCTCGGTAGGGTTAATCGGGCTTCCTCAAAAAGTGCTGTCAAGCTTCAAGAGATAGGACCCAGGATGACTCTTCAACTCATGAAGATTGAGGAAGGATTGTGCTCCGGTGGAGTCATCTTCAGTGAATACGGAAATGGCGATGGGAAGAAAAAGGAAGACGACCACGAAGATGAGGATAACGATGACGAAGATGAAGAAATTGATGAGGAAGATGAGGAAAATGATGAAGAAGAAGATGGTGACGATATTGACCAAAACTAG

Protein Analysis

345

Amino Acids

38.87

Weight (kDa)

6.36

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 46 - 286 1.5e-42 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 376
AciI CCGC 2 cut(s) 208, 376
AclWI GGATC 1 cut(s) 730
AcoI YGGCCR 1 cut(s) 237
AcuI CTGAAG 1 cut(s) 877
AcyI GRCGYC 1 cut(s) 314
AfaI GTAC 2 cut(s) 296, 353
AflII CTTAAG 2 cut(s) 188, 194
AgeI ACCGGT 1 cut(s) 40
AgsI TTSAA 7 cut(s) 13, 149, 232, 469, 686, 821, 848
AjiI CACGTC 1 cut(s) 691
AjnI CCWGG 2 cut(s) 127, 833
AluBI AGCT 6 cut(s) 102, 152, 166, 193, 472, 817
AluI AGCT 6 cut(s) 102, 152, 166, 193, 472, 817
Alw21I GWGCWC 1 cut(s) 878
Alw26I GTCTC 3 cut(s) 216, 384, 631
AlwI GGATC 1 cut(s) 730
AoxI GGCC 3 cut(s) 237, 242, 322
ApeKI GCWGC 1 cut(s) 749
ArsI GACNNNNNNTTYG 2 cut(s) 745, 777
AsiGI ACCGGT 1 cut(s) 40
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 5 cut(s) 70, 131, 242, 323, 830
AsuC2I CCSGG 1 cut(s) 241
AsuHPI GGTGA 1 cut(s) 1025
AvaII GGWCC 3 cut(s) 70, 131, 830
AxyI CCTNAGG 1 cut(s) 299
BaeI ACNNNNGTAYC 2 cut(s) 335, 368
BarI GAAGNNNNNNTAC 2 cut(s) 701, 733
BbsI GAAGAC 1 cut(s) 936
Bbv12I GWGCWC 1 cut(s) 878
BbvI GCAGC 1 cut(s) 761
BccI CCATC 3 cut(s) 666, 908, 1004
BciT130I CCWGG 2 cut(s) 129, 835
BcnI CCSGG 1 cut(s) 241
BcoDI GTCTC 3 cut(s) 216, 384, 631
BfaI CTAG 1 cut(s) 1033
BfrI CTTAAG 2 cut(s) 188, 194
BglII AGATCT 1 cut(s) 406
BisI GCNGC 1 cut(s) 750
BlsI GCNGC 1 cut(s) 751
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 3 cut(s) 129, 241, 835
Bme18I GGWCC 3 cut(s) 70, 131, 830
BmgBI CACGTC 1 cut(s) 691
BmgT120I GGNCC 5 cut(s) 70, 131, 242, 323, 830
BmiI GGNNCC 1 cut(s) 832
BmrFI CCNGG 3 cut(s) 129, 241, 835
BmrI ACTGGG 1 cut(s) 90
BmsI GCATC 3 cut(s) 107, 159, 386
BmuI ACTGGG 1 cut(s) 90
BpiI GAAGAC 1 cut(s) 936
BplI GAGNNNNNCTC 2 cut(s) 301, 333
BpmI CTGGAG 1 cut(s) 474
BpuEI CTTGAG 1 cut(s) 303
BpuMI CCSGG 1 cut(s) 241
BsaHI GRCGYC 1 cut(s) 314
BsaI GGTCTC 1 cut(s) 384
BsaJI CCNNGG 2 cut(s) 95, 833
BsaWI WCCGGW 2 cut(s) 40, 878
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 2 cut(s) 85, 457
Bse21I CCTNAGG 1 cut(s) 299
BseBI CCWGG 2 cut(s) 129, 835
BseDI CCNNGG 2 cut(s) 95, 833
BseGI GGATG 1 cut(s) 843
BseMII CTCAG 3 cut(s) 128, 395, 665
BseNI ACTGG 2 cut(s) 85, 457
BseXI GCAGC 1 cut(s) 761
BseYI CCCAGC 1 cut(s) 400
Bsh1236I CGCG 1 cut(s) 376
Bsh1285I CGRYCG 1 cut(s) 756
BshFI GGCC 3 cut(s) 239, 244, 324
BshTI ACCGGT 1 cut(s) 40
BsiEI CGRYCG 1 cut(s) 756
BsiHKAI GWGCWC 1 cut(s) 878
BsiSI CCGG 3 cut(s) 41, 240, 879
BslFI GGGAC 3 cut(s) 98, 463, 650
BsmAI GTCTC 3 cut(s) 216, 384, 631
BsmFI GGGAC 3 cut(s) 98, 463, 650
BsmI GAATGC 1 cut(s) 424
BsnI GGCC 3 cut(s) 239, 244, 324
Bso31I GGTCTC 1 cut(s) 384
Bsp1286I GDGCHC 1 cut(s) 878
Bsp143I GATC 4 cut(s) 406, 670, 722, 772
BspACI CCGC 2 cut(s) 208, 376
BspANI GGCC 3 cut(s) 239, 244, 324
BspCNI CTCAG 3 cut(s) 129, 394, 666
BspFNI CGCG 1 cut(s) 376
BspHI TCATGA 1 cut(s) 852
BspLI GGNNCC 1 cut(s) 832
BspPI GGATC 1 cut(s) 730
BspTI CTTAAG 2 cut(s) 188, 194
BspTNI GGTCTC 1 cut(s) 384
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 2 cut(s) 85, 457
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 2 cut(s) 95, 833
BssMI GATC 4 cut(s) 406, 670, 722, 772
BssNI GRCGYC 1 cut(s) 314
BssT1I CCWWGG 1 cut(s) 95
Bst2UI CCWGG 2 cut(s) 129, 835
Bst4CI ACNGT 3 cut(s) 486, 523, 757
Bst6I CTCTTC 2 cut(s) 197, 849
BstACI GRCGYC 1 cut(s) 314
BstAFI CTTAAG 2 cut(s) 188, 194
BstC8I GCNNGC 2 cut(s) 7, 60
BstDEI CTNAG 5 cut(s) 137, 218, 299, 381, 674
BstF5I GGATG 1 cut(s) 843
BstFNI CGCG 1 cut(s) 376
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 4 cut(s) 409, 673, 725, 775
BstMAI GTCTC 3 cut(s) 216, 384, 631
BstMBI GATC 4 cut(s) 406, 670, 722, 772
BstMCI CGRYCG 1 cut(s) 756
BstNI CCWGG 2 cut(s) 129, 835
BstSCI CCNGG 3 cut(s) 127, 239, 833
BstUI CGCG 1 cut(s) 376
BstV1I GCAGC 1 cut(s) 761
BstV2I GAAGAC 1 cut(s) 936
BstX2I RGATCY 1 cut(s) 406
BstXI CCANNNNNNTGG 1 cut(s) 80
BstYI RGATCY 1 cut(s) 406
Bsu36I CCTNAGG 1 cut(s) 299
BsuRI GGCC 3 cut(s) 239, 244, 324
BtgZI GCGATG 1 cut(s) 927
BtrI CACGTC 1 cut(s) 691
BtsCI GGATG 1 cut(s) 843
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 4 cut(s) 78, 180, 519, 901
Cac8I GCNNGC 2 cut(s) 7, 60
CciI TCATGA 1 cut(s) 852
CfoI GCGC 1 cut(s) 376
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 5 cut(s) 70, 131, 242, 323, 830
CseI GACGC 2 cut(s) 322, 377
Csp6I GTAC 2 cut(s) 295, 352
CspAI ACCGGT 1 cut(s) 40
CviAII CATG 4 cut(s) 74, 268, 537, 853
CviQI GTAC 2 cut(s) 295, 352
DdeI CTNAG 5 cut(s) 137, 218, 299, 381, 674
DpnI GATC 4 cut(s) 408, 672, 724, 774
DpnII GATC 4 cut(s) 406, 670, 722, 772
EaeI YGGCCR 1 cut(s) 237
Eam1104I CTCTTC 2 cut(s) 197, 849
EarI CTCTTC 2 cut(s) 197, 849
EciI GGCGGA 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 95
Eco31I GGTCTC 1 cut(s) 384
Eco47I GGWCC 3 cut(s) 70, 131, 830
Eco57I CTGAAG 1 cut(s) 877
Eco81I CCTNAGG 1 cut(s) 299
EcoO109I RGGNCCY 1 cut(s) 830
EcoRII CCWGG 2 cut(s) 127, 833
EcoT14I CCWWGG 1 cut(s) 95
ErhI CCWWGG 1 cut(s) 95
FaeI CATG 4 cut(s) 77, 271, 540, 856
FaiI YATR 6 cut(s) 75, 269, 482, 538, 605, 854
FaqI GGGAC 3 cut(s) 98, 463, 650
FatI CATG 4 cut(s) 73, 267, 536, 852
Fnu4HI GCNGC 1 cut(s) 750
FokI GGATG 1 cut(s) 850
Fsp4HI GCNGC 1 cut(s) 750
FspBI CTAG 1 cut(s) 1033
GlaI GCGC 1 cut(s) 375
GluI GCNGC 1 cut(s) 750
GsaI CCCAGC 1 cut(s) 404
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 3 cut(s) 239, 244, 324
HapII CCGG 3 cut(s) 41, 240, 879
HgaI GACGC 2 cut(s) 322, 377
HhaI GCGC 1 cut(s) 376
Hin1I GRCGYC 1 cut(s) 314
Hin1II CATG 4 cut(s) 77, 271, 540, 856
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HindIII AAGCTT 2 cut(s) 191, 815
HinfI GANTC 2 cut(s) 841, 885
HpaI GTTAAC 1 cut(s) 517
HpaII CCGG 3 cut(s) 41, 240, 879
HphI GGTGA 1 cut(s) 1025
Hpy166II GTNNAC 2 cut(s) 70, 517
Hpy188I TCNGA 6 cut(s) 138, 384, 492, 654, 675, 727
Hpy188III TCNNGA 5 cut(s) 114, 686, 706, 821, 853
Hpy8I GTNNAC 2 cut(s) 70, 517
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 3 cut(s) 19, 703, 860
HpyCH4III ACNGT 3 cut(s) 486, 523, 757
HpyCH4IV ACGT 1 cut(s) 690
HpyCH4V TGCA 1 cut(s) 120
HpyF3I CTNAG 5 cut(s) 137, 218, 299, 381, 674
HpySE526I ACGT 1 cut(s) 690
Hsp92I GRCGYC 1 cut(s) 314
Hsp92II CATG 4 cut(s) 77, 271, 540, 856
HspAI GCGC 1 cut(s) 374
KspAI GTTAAC 1 cut(s) 517
Kzo9I GATC 4 cut(s) 406, 670, 722, 772
LmnI GCTCC 3 cut(s) 99, 295, 881
Lsp1109I GCAGC 1 cut(s) 761
LweI GCATC 3 cut(s) 107, 159, 386
MaeI CTAG 1 cut(s) 1033
MaeII ACGT 1 cut(s) 690
MaeIII GTNAC 6 cut(s) 76, 253, 703, 716, 757, 1013
MalI GATC 4 cut(s) 408, 672, 724, 774
MboI GATC 4 cut(s) 406, 670, 722, 772
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 1 cut(s) 878
MluCI AATT 2 cut(s) 559, 972
MlyI GAGTC 2 cut(s) 835, 894
MmeI TCCRAC 1 cut(s) 308
MseI TTAA 8 cut(s) 189, 195, 339, 414, 516, 558, 638, 786
MslI CAYNNNNRTG 1 cut(s) 942
MspCI CTTAAG 2 cut(s) 188, 194
MspI CCGG 3 cut(s) 41, 240, 879
MspR9I CCNGG 3 cut(s) 129, 241, 835
Mva1269I GAATGC 1 cut(s) 424
MvaI CCWGG 2 cut(s) 129, 835
MvnI CGCG 1 cut(s) 376
NciI CCSGG 1 cut(s) 241
NdeII GATC 4 cut(s) 406, 670, 722, 772
NlaIII CATG 4 cut(s) 77, 271, 540, 856
NlaIV GGNNCC 1 cut(s) 832
NmuCI GTSAC 4 cut(s) 76, 703, 757, 1013
PagI TCATGA 1 cut(s) 852
PcsI WCGNNNNNNNCGW 1 cut(s) 909
PctI GAATGC 1 cut(s) 424
PflFI GACNNNGTC 2 cut(s) 74, 701
PinAI ACCGGT 1 cut(s) 40
PkrI GCNGC 1 cut(s) 751
PleI GAGTC 2 cut(s) 835, 893
PpsI GAGTC 2 cut(s) 835, 893
PpuMI RGGWCCY 1 cut(s) 830
Psp5II RGGWCCY 1 cut(s) 830
Psp6I CCWGG 2 cut(s) 127, 833
PspFI CCCAGC 1 cut(s) 400
PspGI CCWGG 2 cut(s) 127, 833
PspN4I GGNNCC 1 cut(s) 832
PspPI GGNCC 5 cut(s) 70, 131, 242, 323, 830
PspPPI RGGWCCY 1 cut(s) 830
PsuI RGATCY 1 cut(s) 406
PsyI GACNNNGTC 2 cut(s) 74, 701
RsaI GTAC 2 cut(s) 296, 353
RsaNI GTAC 2 cut(s) 295, 352
RseI CAYNNNNRTG 1 cut(s) 942
SaqAI TTAA 8 cut(s) 189, 195, 339, 414, 516, 558, 638, 786
SatI GCNGC 1 cut(s) 750
Sau3AI GATC 4 cut(s) 406, 670, 722, 772
Sau96I GGNCC 5 cut(s) 70, 131, 242, 323, 830
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 2 cut(s) 835, 894
ScrFI CCNGG 3 cut(s) 129, 241, 835
SduI GDGCHC 1 cut(s) 878
SetI ASST 9 cut(s) 30, 104, 154, 168, 195, 300, 474, 693, 819
SfaNI GCATC 3 cut(s) 107, 159, 386
SinI GGWCC 3 cut(s) 70, 131, 830
SmiMI CAYNNNNRTG 1 cut(s) 942
SmlI CTYRAG 3 cut(s) 188, 194, 318
SmoI CTYRAG 3 cut(s) 188, 194, 318
Sse9I AATT 2 cut(s) 559, 972
SsiI CCGC 2 cut(s) 208, 376
SspMI CTAG 1 cut(s) 1033
StyD4I CCNGG 3 cut(s) 127, 239, 833
StyI CCWWGG 1 cut(s) 95
TaaI ACNGT 3 cut(s) 486, 523, 757
TaiI ACGT 1 cut(s) 693
TaqI TCGA 3 cut(s) 50, 275, 394
TasI AATT 2 cut(s) 559, 972
TatI WGTACW 1 cut(s) 351
Tru1I TTAA 8 cut(s) 189, 195, 339, 414, 516, 558, 638, 786
Tru9I TTAA 8 cut(s) 189, 195, 339, 414, 516, 558, 638, 786
TscAI CASTG 4 cut(s) 85, 187, 526, 901
TseFI GTSAC 4 cut(s) 76, 703, 757, 1013
TseI GCWGC 1 cut(s) 749
Tsp45I GTSAC 4 cut(s) 76, 703, 757, 1013
TspDTI ATGAA 4 cut(s) 525, 869, 981, 1014
TspGWI ACGGA 3 cut(s) 282, 735, 918
TspRI CASTG 4 cut(s) 85, 187, 526, 901
Tth111I GACNNNGTC 2 cut(s) 74, 701
Vha464I CTTAAG 2 cut(s) 188, 194
VpaK11BI GGWCC 3 cut(s) 70, 131, 830
XspI CTAG 1 cut(s) 1033
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.