Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008352

Physical Location & Seq

Genomic Coordinates Reverse (-)

36680075 .. 36682220

Gene Structure 2146 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1288000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1032 bp

ATGGCTCGCTTCAAAAATAAGAAGAAGGTGTTTGTGAAGCCGGTTTCGACGAAGAAGCAGGCTAATGTGGACCATATCACAGGGGATAAAATCCCAAGGAGCTTTGTGTTTTCCAGATGCAAGTTGCCTGGTCCTCTGAGGCAGCTTCAAGCTGATTTGAGGAAGCTCATGCTTCCCTACACTGCCCTTAAGCTTAAGGAGAAGAGGCGGAACAATCTTAGAGACTTTTTGAATGTGGCCGGGCCTATGGGGGTTACACATTTCCTCATGTTGTCGAAAACTCCAACTGCGCCATACCTTAGGGTTGCGAGGACACCTCAAGGCCCAACGCTTACATTTAAGATACAGGAGTACTCTTTGGCGGTTGATATTGCGCGGTCTCAAAAGCGTCCTAGATGCCCGGCAGATCTTTTTAAGAATGCCCCTTTGATTGTTCTTTCTGGTTTTGGGACCGGAGAGCAACATTTGAAGCTCACAACGATACTGTTTCAGAACATCTTTCCAGCCATTGATGTTAACACTGTGAAACTTTCTTCATGCCAAAGAATAGTGTTGCTTAATTACAACAAGGACACAAAACTGATTGATTTCCGGCATTACTCCATAAGATTACAGCCTGTGGGTGTCTCCCAGAGATTAAGAAGATTTGTTCAGAACCATCAAGTCCCTGATCTAAGAAATCTTCAAGACGTGAGTGACTTCGTCACAAAGGCTGGTTACGGATCAGAAAGTGAAGCAGATGATGAGGCAGCGACCGTGACTTTGGCTAATGATCTAGGTAGGGTTAATCGGGCTTCCTCAAAAAGTGCTGTCAAGCTTCAAGAGATAGGACCCAGAATGACCCTTCAACTCATGAAGATTGAGGAAGGATTGTGCACCGGTGGAGTCATCTTCAGTGAATATGGAAATGGCGATGGGAAGAAAAAGGAAGACAACCATGAAGATGAGGAAAACGATGACGAAGACGAAGAAATTGATGATGAAGATGAGGAAAATGATGAAGAAGATGGTGAAGATATCGACGAAGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000027 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0006996 GO:0008150 GO:0009987 GO:0016043 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0030684 GO:0030687 GO:0032991 GO:0034622 GO:0042254 GO:0042255 GO:0042273 GO:0043933 GO:0044085 GO:0065003 GO:0070925 GO:0071826 GO:0071840 GO:0097159 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Brix

Protein Analysis

344

Amino Acids

38.79

Weight (kDa)

6.36

Isoelectric Point (pI)

48.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Brix	PF04427	46 - 286	1.5e-42	Brix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008352)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61770 AT5G61770 AT5G61770
fragaria_vesca	FvH4_2g25700
malus_domestica	MD03G1288000.v1.1 MD11G1310300.v1.1
prunus_persica	Prupe.8G261200_v2.0.a1 Prupe.8G261200_v2.0.a1 Prupe.8G261200_v2.0.a1
pyrus_communis	pycom03g22800 pycom11g27110 pycom16g13360
rosa_chinensis	RchiOBHm_Chr6g0293981
rosa_laevigata	RLG00000011910
rosa_multiflora	Rmu_sc0005126.1_g000007 Rmu_sc0005647.1_g000003
rosa_roxburghii	Rroxscaffold_7G00173770
rosa_rugosa	Rorug06G0239800
rosa_samantha	Rh6AG352800 Rh6BG359800 Rh6CG367100 Rh6DG353500
rosa_wichuraiana	Rw6G030740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	376
AciI	CCGC	3 cut(s)	208, 362, 376
AclWI	GGATC	1 cut(s)	730
AcoI	YGGCCR	1 cut(s)	237
AcuI	CTGAAG	1 cut(s)	877
AfaI	GTAC	1 cut(s)	353
AflII	CTTAAG	2 cut(s)	188, 194
AgeI	ACCGGT	1 cut(s)	878
AgsI	TTSAA	7 cut(s)	13, 149, 232, 469, 686, 821, 848
AjiI	CACGTC	1 cut(s)	691
AjnI	CCWGG	1 cut(s)	127
AluBI	AGCT	7 cut(s)	102, 145, 152, 166, 193, 472, 817
AluI	AGCT	7 cut(s)	102, 145, 152, 166, 193, 472, 817
Alw21I	GWGCWC	1 cut(s)	878
Alw26I	GTCTC	3 cut(s)	216, 384, 631
Alw44I	GTGCAC	1 cut(s)	874
AlwI	GGATC	1 cut(s)	730
AoxI	GGCC	3 cut(s)	237, 242, 322
ApaLI	GTGCAC	1 cut(s)	874
ApeKI	GCWGC	2 cut(s)	142, 749
ArsI	GACNNNNNNTTYG	2 cut(s)	745, 777
AsiGI	ACCGGT	1 cut(s)	878
AspLEI	GCGC	2 cut(s)	292, 376
AspS9I	GGNCC	6 cut(s)	70, 131, 242, 323, 450, 830
AsuC2I	CCSGG	2 cut(s)	241, 401
AsuHPI	GGTGA	1 cut(s)	1022
AvaII	GGWCC	4 cut(s)	70, 131, 450, 830
AxyI	CCTNAGG	1 cut(s)	299
BaeGI	GKGCMC	1 cut(s)	878
BaeI	ACNNNNGTAYC	2 cut(s)	335, 368
BbsI	GAAGAC	2 cut(s)	936, 969
Bbv12I	GWGCWC	1 cut(s)	878
BbvI	GCAGC	2 cut(s)	154, 761
BccI	CCATC	3 cut(s)	666, 908, 1001
BciT130I	CCWGG	1 cut(s)	129
BcnI	CCSGG	2 cut(s)	241, 401
BcoDI	GTCTC	3 cut(s)	216, 384, 631
BfaI	CTAG	2 cut(s)	393, 776
BfrI	CTTAAG	2 cut(s)	188, 194
BglII	AGATCT	1 cut(s)	406
BisI	GCNGC	2 cut(s)	143, 750
BlsI	GCNGC	2 cut(s)	144, 751
BmcAI	AGTACT	1 cut(s)	353
Bme1390I	CCNGG	3 cut(s)	129, 241, 401
Bme18I	GGWCC	4 cut(s)	70, 131, 450, 830
BmgBI	CACGTC	1 cut(s)	691
BmgT120I	GGNCC	6 cut(s)	70, 131, 242, 323, 450, 830
BmiI	GGNNCC	2 cut(s)	451, 832
BmrFI	CCNGG	3 cut(s)	129, 241, 401
BmsI	GCATC	2 cut(s)	107, 386
BpiI	GAAGAC	2 cut(s)	936, 969
BplI	GAGNNNNNCTC	2 cut(s)	301, 333
BpuEI	CTTGAG	1 cut(s)	303
BpuMI	CCSGG	2 cut(s)	241, 401
BsaI	GGTCTC	1 cut(s)	384
BsaJI	CCNNGG	1 cut(s)	95
BsaWI	WCCGGW	2 cut(s)	452, 878
Bse118I	RCCGGY	2 cut(s)	40, 878
Bse21I	CCTNAGG	1 cut(s)	299
BseBI	CCWGG	1 cut(s)	129
BseDI	CCNNGG	1 cut(s)	95
BseMII	CTCAG	1 cut(s)	128
BseSI	GKGCMC	1 cut(s)	878
BseXI	GCAGC	2 cut(s)	154, 761
Bsh1236I	CGCG	1 cut(s)	376
Bsh1285I	CGRYCG	1 cut(s)	756
BshFI	GGCC	3 cut(s)	239, 244, 324
BshTI	ACCGGT	1 cut(s)	878
BsiEI	CGRYCG	1 cut(s)	756
BsiHKAI	GWGCWC	1 cut(s)	878
BsiSI	CCGG	6 cut(s)	41, 240, 401, 453, 592, 879
BslFI	GGGAC	2 cut(s)	463, 650
BsmAI	GTCTC	3 cut(s)	216, 384, 631
BsmFI	GGGAC	2 cut(s)	463, 650
BsmI	GAATGC	1 cut(s)	424
BsnI	GGCC	3 cut(s)	239, 244, 324
Bso31I	GGTCTC	1 cut(s)	384
Bsp1286I	GDGCHC	1 cut(s)	878
Bsp143I	GATC	4 cut(s)	406, 670, 722, 772
BspACI	CCGC	3 cut(s)	208, 362, 376
BspANI	GGCC	3 cut(s)	239, 244, 324
BspCNI	CTCAG	1 cut(s)	129
BspFNI	CGCG	1 cut(s)	376
BspHI	TCATGA	1 cut(s)	852
BspLI	GGNNCC	2 cut(s)	451, 832
BspPI	GGATC	1 cut(s)	730
BspTI	CTTAAG	2 cut(s)	188, 194
BspTNI	GGTCTC	1 cut(s)	384
BsrFI	RCCGGY	2 cut(s)	40, 878
BssAI	RCCGGY	2 cut(s)	40, 878
BssECI	CCNNGG	1 cut(s)	95
BssMI	GATC	4 cut(s)	406, 670, 722, 772
BssT1I	CCWWGG	1 cut(s)	95
Bst2UI	CCWGG	1 cut(s)	129
Bst4CI	ACNGT	3 cut(s)	486, 523, 757
Bst6I	CTCTTC	1 cut(s)	197
BstAFI	CTTAAG	2 cut(s)	188, 194
BstC8I	GCNNGC	2 cut(s)	7, 60
BstDEI	CTNAG	4 cut(s)	137, 218, 299, 674
BstFNI	CGCG	1 cut(s)	376
BstHHI	GCGC	2 cut(s)	292, 376
BstKTI	GATC	4 cut(s)	409, 673, 725, 775
BstMAI	GTCTC	3 cut(s)	216, 384, 631
BstMBI	GATC	4 cut(s)	406, 670, 722, 772
BstMCI	CGRYCG	1 cut(s)	756
BstNI	CCWGG	1 cut(s)	129
BstSCI	CCNGG	3 cut(s)	127, 239, 399
BstSLI	GKGCMC	1 cut(s)	878
BstUI	CGCG	1 cut(s)	376
BstV1I	GCAGC	2 cut(s)	154, 761
BstV2I	GAAGAC	2 cut(s)	936, 969
BstX2I	RGATCY	1 cut(s)	406
BstYI	RGATCY	1 cut(s)	406
Bsu36I	CCTNAGG	1 cut(s)	299
BsuRI	GGCC	3 cut(s)	239, 244, 324
BtgZI	GCGATG	1 cut(s)	927
BtrI	CACGTC	1 cut(s)	691
BtsI	GCAGTG	1 cut(s)	180
BtsIMutI	CAGTG	3 cut(s)	180, 519, 901
Cac8I	GCNNGC	2 cut(s)	7, 60
CciI	TCATGA	1 cut(s)	852
CfoI	GCGC	2 cut(s)	292, 376
Cfr10I	RCCGGY	2 cut(s)	40, 878
Cfr13I	GGNCC	6 cut(s)	70, 131, 242, 323, 450, 830
CseI	GACGC	1 cut(s)	377
Csp6I	GTAC	1 cut(s)	352
CspAI	ACCGGT	1 cut(s)	878
CviAII	CATG	5 cut(s)	169, 268, 537, 853, 938
CviQI	GTAC	1 cut(s)	352
DdeI	CTNAG	4 cut(s)	137, 218, 299, 674
DpnI	GATC	4 cut(s)	408, 672, 724, 774
DpnII	GATC	4 cut(s)	406, 670, 722, 772
EaeI	YGGCCR	1 cut(s)	237
Eam1104I	CTCTTC	1 cut(s)	197
EarI	CTCTTC	1 cut(s)	197
EciI	GGCGGA	1 cut(s)	223
Eco130I	CCWWGG	1 cut(s)	95
Eco31I	GGTCTC	1 cut(s)	384
Eco32I	GATATC	1 cut(s)	1018
Eco47I	GGWCC	4 cut(s)	70, 131, 450, 830
Eco57I	CTGAAG	1 cut(s)	877
Eco81I	CCTNAGG	1 cut(s)	299
EcoO109I	RGGNCCY	1 cut(s)	830
EcoRII	CCWGG	1 cut(s)	127
EcoRV	GATATC	1 cut(s)	1018
EcoT14I	CCWWGG	1 cut(s)	95
ErhI	CCWWGG	1 cut(s)	95
FaeI	CATG	5 cut(s)	172, 271, 540, 856, 941
FaqI	GGGAC	2 cut(s)	463, 650
FatI	CATG	5 cut(s)	168, 267, 536, 852, 937
Fnu4HI	GCNGC	2 cut(s)	143, 750
Fsp4HI	GCNGC	2 cut(s)	143, 750
FspBI	CTAG	2 cut(s)	393, 776
GlaI	GCGC	2 cut(s)	291, 375
GluI	GCNGC	2 cut(s)	143, 750
HaeIII	GGCC	3 cut(s)	239, 244, 324
HapII	CCGG	6 cut(s)	41, 240, 401, 453, 592, 879
HgaI	GACGC	1 cut(s)	377
HhaI	GCGC	2 cut(s)	292, 376
Hin1II	CATG	5 cut(s)	172, 271, 540, 856, 941
Hin6I	GCGC	2 cut(s)	290, 374
HinP1I	GCGC	2 cut(s)	290, 374
HincII	GTYRAC	1 cut(s)	517
HindII	GTYRAC	1 cut(s)	517
HindIII	AAGCTT	2 cut(s)	191, 815
HinfI	GANTC	1 cut(s)	885
HpaI	GTTAAC	1 cut(s)	517
HpaII	CCGG	6 cut(s)	41, 240, 401, 453, 592, 879
HphI	GGTGA	1 cut(s)	1022
Hpy166II	GTNNAC	3 cut(s)	70, 517, 876
Hpy188I	TCNGA	4 cut(s)	138, 492, 654, 727
Hpy188III	TCNNGA	4 cut(s)	114, 686, 821, 853
Hpy8I	GTNNAC	3 cut(s)	70, 517, 876
Hpy99I	CGWCG	2 cut(s)	52, 1025
HpyAV	CCTTC	3 cut(s)	19, 854, 860
HpyCH4III	ACNGT	3 cut(s)	486, 523, 757
HpyCH4IV	ACGT	1 cut(s)	690
HpyCH4V	TGCA	2 cut(s)	120, 876
HpyF3I	CTNAG	4 cut(s)	137, 218, 299, 674
HpySE526I	ACGT	1 cut(s)	690
Hsp92II	CATG	5 cut(s)	172, 271, 540, 856, 941
HspAI	GCGC	2 cut(s)	290, 374
KspAI	GTTAAC	1 cut(s)	517
Kzo9I	GATC	4 cut(s)	406, 670, 722, 772
LmnI	GCTCC	1 cut(s)	99
Lsp1109I	GCAGC	2 cut(s)	154, 761
LweI	GCATC	2 cut(s)	107, 386
MaeI	CTAG	2 cut(s)	393, 776
MaeII	ACGT	1 cut(s)	690
MaeIII	GTNAC	5 cut(s)	253, 695, 703, 716, 757
MalI	GATC	4 cut(s)	408, 672, 724, 774
MboI	GATC	4 cut(s)	406, 670, 722, 772
MflI	RGATCY	1 cut(s)	406
MhlI	GDGCHC	1 cut(s)	878
MluCI	AATT	2 cut(s)	559, 972
MlyI	GAGTC	1 cut(s)	894
MmeI	TCCRAC	1 cut(s)	308
MseI	TTAA	8 cut(s)	189, 195, 339, 414, 516, 558, 638, 786
MslI	CAYNNNNRTG	1 cut(s)	942
MspCI	CTTAAG	2 cut(s)	188, 194
MspI	CCGG	6 cut(s)	41, 240, 401, 453, 592, 879
MspR9I	CCNGG	3 cut(s)	129, 241, 401
Mva1269I	GAATGC	1 cut(s)	424
MvaI	CCWGG	1 cut(s)	129
MvnI	CGCG	1 cut(s)	376
NciI	CCSGG	2 cut(s)	241, 401
NdeII	GATC	4 cut(s)	406, 670, 722, 772
NlaIII	CATG	5 cut(s)	172, 271, 540, 856, 941
NlaIV	GGNNCC	2 cut(s)	451, 832
NmuCI	GTSAC	3 cut(s)	695, 703, 757
PagI	TCATGA	1 cut(s)	852
PctI	GAATGC	1 cut(s)	424
PflFI	GACNNNGTC	1 cut(s)	701
PinAI	ACCGGT	1 cut(s)	878
PkrI	GCNGC	2 cut(s)	144, 751
PleI	GAGTC	1 cut(s)	893
PpsI	GAGTC	1 cut(s)	893
PpuMI	RGGWCCY	1 cut(s)	830
Psp5II	RGGWCCY	1 cut(s)	830
Psp6I	CCWGG	1 cut(s)	127
PspGI	CCWGG	1 cut(s)	127
PspN4I	GGNNCC	2 cut(s)	451, 832
PspPI	GGNCC	6 cut(s)	70, 131, 242, 323, 450, 830
PspPPI	RGGWCCY	1 cut(s)	830
PsuI	RGATCY	1 cut(s)	406
PsyI	GACNNNGTC	1 cut(s)	701
RsaI	GTAC	1 cut(s)	353
RsaNI	GTAC	1 cut(s)	352
RseI	CAYNNNNRTG	1 cut(s)	942
SaqAI	TTAA	8 cut(s)	189, 195, 339, 414, 516, 558, 638, 786
SatI	GCNGC	2 cut(s)	143, 750
Sau3AI	GATC	4 cut(s)	406, 670, 722, 772
Sau96I	GGNCC	6 cut(s)	70, 131, 242, 323, 450, 830
ScaI	AGTACT	1 cut(s)	353
SchI	GAGTC	1 cut(s)	894
ScrFI	CCNGG	3 cut(s)	129, 241, 401
SduI	GDGCHC	1 cut(s)	878
SfaNI	GCATC	2 cut(s)	107, 386
SgrAI	CRCCGGYG	1 cut(s)	878
SinI	GGWCC	4 cut(s)	70, 131, 450, 830
SmiMI	CAYNNNNRTG	1 cut(s)	942
SmlI	CTYRAG	3 cut(s)	188, 194, 318
SmoI	CTYRAG	3 cut(s)	188, 194, 318
Sse9I	AATT	2 cut(s)	559, 972
SsiI	CCGC	3 cut(s)	208, 362, 376
SspMI	CTAG	2 cut(s)	393, 776
StyD4I	CCNGG	3 cut(s)	127, 239, 399
StyI	CCWWGG	1 cut(s)	95
TaaI	ACNGT	3 cut(s)	486, 523, 757
TaiI	ACGT	1 cut(s)	693
TaqI	TCGA	3 cut(s)	47, 275, 1020
TasI	AATT	2 cut(s)	559, 972
TatI	WGTACW	1 cut(s)	351
Tru1I	TTAA	8 cut(s)	189, 195, 339, 414, 516, 558, 638, 786
Tru9I	TTAA	8 cut(s)	189, 195, 339, 414, 516, 558, 638, 786
TscAI	CASTG	3 cut(s)	187, 526, 901
TseFI	GTSAC	3 cut(s)	695, 703, 757
TseI	GCWGC	2 cut(s)	142, 749
Tsp45I	GTSAC	3 cut(s)	695, 703, 757
TspDTI	ATGAA	5 cut(s)	525, 869, 954, 996, 1014
TspGWI	ACGGA	1 cut(s)	735
TspRI	CASTG	3 cut(s)	187, 526, 901
Tth111I	GACNNNGTC	1 cut(s)	701
Vha464I	CTTAAG	2 cut(s)	188, 194
VneI	GTGCAC	1 cut(s)	874
VpaK11BI	GGWCC	4 cut(s)	70, 131, 450, 830
XspI	CTAG	2 cut(s)	393, 776
ZrmI	AGTACT	1 cut(s)	353

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.