FvH4_2g26010

Exopolygalacturonase clone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016557
Physical Location & Seq
Forward (+)
20961536 .. 20964058
2523 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26010.t1

Sequence Viewer

Length: 1476 bp
ATGTTTTCCTACTTAGCCATTCCAGAAAAAATAAAAAAATATATAAAAGATAAAATTAAAAATCTAGTACATAGGAAGATGTCTATGGGACTGAGATCGCCGAACACGGTCATCGCAAGGAGATTAGATGCTATTATTCTCGTAGGCTTAGTTTTTCTTTCTTGCATGTCCGATGGTGCATCCCGTGGACATCTTTTTGGGGCTGGTGCACTACGTCGGAGCCTCACTGATACTACGAACCGAAAAGTATTTGACGTCACAGCCTTCGGTGCTAAGCCTGAATCCGGAAAAGGTCGACAAATGAAAGAGCTTCCTGGCGGTGGAGATCCAACTAAACCACAACCTGATGATGGCGGCGACGGGGATGACAATGATACTGGAATGGCATTCATTAAGACGTGGGTGGCCGCATGCAGAGGAGAGTACAAGGGTCCAGCAAAGGTTGTTGTTCCAAAGGGCACATTTTTGATTGGACCTGTGGTGTTCCAAGGCCCTTGCACCAGCTCCAAGGAGCCAATCATGGTGGAAATTCAGGGAGAGGTGAAAGCCACCACCGATTTGAGTGATTACACCTCTCCTGAATGGATCACGTTCGAAAATGTTGACGGTCTGATCGTCACGGGCGACGGAGTTATGAATGGTCAAGGCGAGAAAACATGGAACGGAAATGGCTGCGATAAGGACGACTGCCCCCATGCCCCATCCAACATGAAATTCATCAAGGTTAAGAATGCACTTATAGAGAAGATCACATCCTTGAACAGCAAGTACTTCCATTACCACATCACCGTCTCCAGCAGAGTTACCATAGACAGTGTGCACATCACTGCTCCCGGCGAAAGCCCTAACACCGACGGCTGCCACATCAGTGATTCCGACAATGTTAAAGTGATCAACAGCATCATTGGAACCGGAGACGACTGCGTTTCCATCGGCAAGGGTGCCCGCCAAATCACCGTCAACAACGTTACTTGCGGCCCTGGCCATGGCATCAGTGTTGGAAGTCTTGGAAAATACAAGAACGAACAACCCGTGGTTGGTGTTACTGTGACCAACTGCAAACTGGTGAACACAACCAACGGCGCCAGAATCAAAACTTGGGCCAACAACTACGGAGGAGACGCAAGCGGAATTACATTCGATAACCTCGTCATGGAGCATGTGAAAGCCCCAATCGTCATCGATCAAAGCTATGGAACCAAGAAAGATCCGAAAAACCCTAACGCCGGAAAGCAATCCCTGTATAAGCTCCATGATATTCACTTCAGAAACATTAAGGGCACCTCGTCGCAAAACGTTGCCATGTCACTGGCATGCAGCGCTACAAAACCTTGTGAGGGGGTTGAGTTGAGCGAGATCGACATTTCCTACGGAGGCGCCAGCAACTCCAAAAAGACGGACATTGCATCGGAATGTTACAACGCAAAGGTTACAACTAAGGGCAAAATAAATCCAGCAGCACCAGCTTGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0016787 GO:0016798 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

492

Amino Acids

52.5

Weight (kDa)

8.77

Isoelectric Point (pI)

34.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 145 - 470 8.4e-89 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016557)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g26010
malus_domestica MD03G1291400.v1.1 MD11G1306900.v1.1
prunus_persica Prupe.8G264500_v2.0.a1
pyrus_communis pycom03g23080 pycom11g26850
rosa_chinensis RchiOBHm_Chr6g0294381
rosa_laevigata RLG00000011876
rosa_multiflora Rmu_sc0005369.1_g000014
rosa_roxburghii Rroxscaffold_7G00173430
rosa_rugosa Rorug06G0243900
rosa_samantha Rh6AG356500 Rh6CG370500
rosa_wichuraiana Rw6G031070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 258
AccB1I GGYRCC 4 cut(s) 941, 1082, 1280, 1376
AccI GTMKAC 1 cut(s) 295
AccIII TCCGGA 1 cut(s) 284
AciI CCGC 6 cut(s) 318, 354, 408, 946, 975, 1128
AclI AACGTT 2 cut(s) 966, 1296
AclWI GGATC 3 cut(s) 320, 593, 1202
AcoI YGGCCR 2 cut(s) 405, 982
AcsI RAATTY 2 cut(s) 528, 713
AcuI CTGAAG 1 cut(s) 1249
AcyI GRCGYC 3 cut(s) 255, 1083, 1377
AfaI GTAC 3 cut(s) 69, 425, 770
AfeI AGCGCT 1 cut(s) 1321
AfiI CCNNNNNNNGG 8 cut(s) 284, 320, 350, 986, 1037, 1153, 1226, 1337
AgsI TTSAA 1 cut(s) 760
AjiI CACGTC 1 cut(s) 399
AjnI CCWGG 2 cut(s) 313, 979
AjuI GAANNNNNNNTTGG 4 cut(s) 698, 730, 1081, 1113
AleI CACNNNNGTG 1 cut(s) 867
AluBI AGCT 5 cut(s) 310, 504, 1191, 1249, 1466
AluI AGCT 5 cut(s) 310, 504, 1191, 1249, 1466
Alw21I GWGCWC 2 cut(s) 211, 822
Alw26I GTCTC 3 cut(s) 796, 909, 1113
Alw44I GTGCAC 2 cut(s) 207, 818
AlwI GGATC 3 cut(s) 320, 593, 1202
Aor13HI TCCGGA 1 cut(s) 284
Aor51HI AGCGCT 1 cut(s) 1321
AoxI GGCC 5 cut(s) 405, 490, 976, 982, 1101
ApaLI GTGCAC 2 cut(s) 207, 818
ApeKI GCWGC 4 cut(s) 672, 858, 1317, 1457
ApoI RAATTY 2 cut(s) 528, 713
AspLEI GCGC 3 cut(s) 1085, 1322, 1379
AspS9I GGNCC 5 cut(s) 431, 473, 491, 977, 1101
AsuC2I CCSGG 1 cut(s) 834
AsuHPI GGTGA 4 cut(s) 553, 778, 946, 1078
AsuII TTCGAA 1 cut(s) 594
AvaII GGWCC 2 cut(s) 431, 473
BaeGI GKGCMC 5 cut(s) 211, 461, 822, 946, 1283
BalI TGGCCA 1 cut(s) 984
BanI GGYRCC 4 cut(s) 941, 1082, 1280, 1376
Bbv12I GWGCWC 2 cut(s) 211, 822
BbvI GCAGC 4 cut(s) 659, 845, 1329, 1469
BccI CCATC 4 cut(s) 167, 344, 709, 938
BceAI ACGGC 2 cut(s) 871, 1096
BciT130I CCWGG 2 cut(s) 315, 981
BclI TGATCA 1 cut(s) 891
BcnI CCSGG 1 cut(s) 834
BcoDI GTCTC 3 cut(s) 796, 909, 1113
BfaI CTAG 1 cut(s) 65
BfoI RGCGCY 3 cut(s) 1086, 1323, 1380
BisI GCNGC 7 cut(s) 355, 408, 673, 859, 976, 1318, 1458
BlpI GCTNAGC 1 cut(s) 273
BlsI GCNGC 7 cut(s) 356, 409, 674, 860, 977, 1319, 1459
BmcAI AGTACT 1 cut(s) 770
Bme1390I CCNGG 3 cut(s) 315, 834, 981
Bme18I GGWCC 2 cut(s) 431, 473
BmgBI CACGTC 1 cut(s) 399
BmgT120I GGNCC 5 cut(s) 431, 473, 491, 977, 1101
BmiI GGNNCC 9 cut(s) 221, 432, 513, 910, 943, 1084, 1198, 1282, 1378
BmrFI CCNGG 3 cut(s) 315, 834, 981
BmsI GCATC 5 cut(s) 118, 188, 909, 999, 1415
BpmI CTGGAG 1 cut(s) 778
Bpu1102I GCTNAGC 1 cut(s) 273
Bpu14I TTCGAA 1 cut(s) 594
BpuMI CCSGG 1 cut(s) 834
Bsa29I ATCGAT 1 cut(s) 1182
BsaHI GRCGYC 3 cut(s) 255, 1083, 1377
BsaJI CCNNGG 6 cut(s) 184, 487, 507, 979, 985, 1032
BsaWI WCCGGW 2 cut(s) 284, 911
Bsc4I CCNNNNNNNGG 8 cut(s) 284, 320, 350, 986, 1037, 1153, 1226, 1337
Bse1I ACTGG 3 cut(s) 382, 1068, 1314
Bse3DI GCAATG 1 cut(s) 1401
BseAI TCCGGA 1 cut(s) 284
BseBI CCWGG 2 cut(s) 315, 981
BseCI ATCGAT 1 cut(s) 1182
BseDI CCNNGG 6 cut(s) 184, 487, 507, 979, 985, 1032
BseGI GGATG 4 cut(s) 179, 370, 701, 752
BseLI CCNNNNNNNGG 8 cut(s) 284, 320, 350, 986, 1037, 1153, 1226, 1337
BseMI GCAATG 1 cut(s) 1401
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 3 cut(s) 382, 1068, 1314
BseRI GAGGAG 2 cut(s) 432, 1131
BseSI GKGCMC 5 cut(s) 211, 461, 822, 946, 1283
BseXI GCAGC 4 cut(s) 659, 845, 1329, 1469
BshFI GGCC 5 cut(s) 407, 492, 978, 984, 1103
BshNI GGYRCC 4 cut(s) 941, 1082, 1280, 1376
BshVI ATCGAT 1 cut(s) 1182
BsiHKAI GWGCWC 2 cut(s) 211, 822
BsiSI CCGG 4 cut(s) 285, 834, 912, 1227
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 8 cut(s) 284, 320, 350, 986, 1037, 1153, 1226, 1337
BsmAI GTCTC 3 cut(s) 796, 909, 1113
BsmBI CGTCTC 3 cut(s) 796, 909, 1113
BsmFI GGGAC 1 cut(s) 102
BsmI GAATGC 2 cut(s) 386, 736
BsnI GGCC 5 cut(s) 407, 492, 978, 984, 1103
Bsp119I TTCGAA 1 cut(s) 594
Bsp1286I GDGCHC 5 cut(s) 211, 461, 822, 946, 1283
Bsp13I TCCGGA 1 cut(s) 284
Bsp143I GATC 9 cut(s) 95, 325, 585, 612, 747, 891, 1183, 1207, 1356
Bsp1720I GCTNAGC 1 cut(s) 273
Bsp19I CCATGG 1 cut(s) 985
BspACI CCGC 6 cut(s) 318, 354, 408, 946, 975, 1128
BspANI GGCC 5 cut(s) 407, 492, 978, 984, 1103
BspCNI CTCAG 1 cut(s) 84
BspDI ATCGAT 1 cut(s) 1182
BspEI TCCGGA 1 cut(s) 284
BspLI GGNNCC 9 cut(s) 221, 432, 513, 910, 943, 1084, 1198, 1282, 1378
BspPI GGATC 3 cut(s) 320, 593, 1202
BspT104I TTCGAA 1 cut(s) 594
BspT107I GGYRCC 4 cut(s) 941, 1082, 1280, 1376
BsrDI GCAATG 1 cut(s) 1401
BsrI ACTGG 3 cut(s) 382, 1068, 1314
BssECI CCNNGG 6 cut(s) 184, 487, 507, 979, 985, 1032
BssMI GATC 9 cut(s) 95, 325, 585, 612, 747, 891, 1183, 1207, 1356
BssNI GRCGYC 3 cut(s) 255, 1083, 1377
BssT1I CCWWGG 3 cut(s) 487, 507, 985
Bst2UI CCWGG 2 cut(s) 315, 981
Bst4CI ACNGT 6 cut(s) 109, 608, 790, 815, 958, 1048
BstACI GRCGYC 3 cut(s) 255, 1083, 1377
BstBI TTCGAA 1 cut(s) 594
BstC8I GCNNGC 6 cut(s) 412, 946, 1126, 1315, 1381, 1468
BstDEI CTNAG 5 cut(s) 13, 92, 148, 273, 1437
BstDSI CCRYGG 3 cut(s) 184, 985, 1032
BstF5I GGATG 4 cut(s) 179, 370, 701, 752
BstH2I RGCGCY 3 cut(s) 1086, 1323, 1380
BstHHI GCGC 3 cut(s) 1085, 1322, 1379
BstKTI GATC 9 cut(s) 98, 328, 588, 615, 750, 894, 1186, 1210, 1359
BstMAI GTCTC 3 cut(s) 796, 909, 1113
BstMBI GATC 9 cut(s) 95, 325, 585, 612, 747, 891, 1183, 1207, 1356
BstMWI GCNNNNNNNGC 4 cut(s) 269, 981, 1319, 1463
BstNI CCWGG 2 cut(s) 315, 981
BstNSI RCATGY 4 cut(s) 169, 414, 1163, 1317
BstSCI CCNGG 3 cut(s) 313, 832, 979
BstSLI GKGCMC 5 cut(s) 211, 461, 822, 946, 1283
BstV1I GCAGC 4 cut(s) 659, 845, 1329, 1469
BstX2I RGATCY 2 cut(s) 325, 1207
BstXI CCANNNNNNTGG 1 cut(s) 1309
BstYI RGATCY 2 cut(s) 325, 1207
Bsu15I ATCGAT 1 cut(s) 1182
BsuRI GGCC 5 cut(s) 407, 492, 978, 984, 1103
BsuTUI ATCGAT 1 cut(s) 1182
BtgI CCRYGG 3 cut(s) 184, 985, 1032
BtgZI GCGATG 1 cut(s) 97
BtrI CACGTC 1 cut(s) 399
BtsCI GGATG 4 cut(s) 179, 370, 701, 752
BtsI GCAGTG 1 cut(s) 825
BtsIMutI CAGTG 6 cut(s) 225, 820, 825, 874, 1000, 1307
Cac8I GCNNGC 6 cut(s) 412, 946, 1126, 1315, 1381, 1468
CfoI GCGC 3 cut(s) 1085, 1322, 1379
Cfr13I GGNCC 5 cut(s) 431, 473, 491, 977, 1101
ClaI ATCGAT 1 cut(s) 1182
CseI GACGC 1 cut(s) 1130
Csp6I GTAC 3 cut(s) 68, 424, 769
CspCI CAANNNNNGTGG 2 cut(s) 504, 539
CviQI GTAC 3 cut(s) 68, 424, 769
DdeI CTNAG 5 cut(s) 13, 92, 148, 273, 1437
DinI GGCGCC 2 cut(s) 1084, 1378
DpnI GATC 9 cut(s) 97, 327, 587, 614, 749, 893, 1185, 1209, 1358
DpnII GATC 9 cut(s) 95, 325, 585, 612, 747, 891, 1183, 1207, 1356
EaeI YGGCCR 2 cut(s) 405, 982
Eco130I CCWWGG 3 cut(s) 487, 507, 985
Eco47I GGWCC 2 cut(s) 431, 473
Eco47III AGCGCT 1 cut(s) 1321
Eco57I CTGAAG 1 cut(s) 1249
EcoO109I RGGNCCY 1 cut(s) 491
EcoRII CCWGG 2 cut(s) 313, 979
EcoT14I CCWWGG 3 cut(s) 487, 507, 985
EgeI GGCGCC 2 cut(s) 1084, 1378
EheI GGCGCC 2 cut(s) 1084, 1378
ErhI CCWWGG 3 cut(s) 487, 507, 985
Esp3I CGTCTC 3 cut(s) 796, 909, 1113
FaqI GGGAC 1 cut(s) 102
FauI CCCGC 1 cut(s) 953
FbaI TGATCA 1 cut(s) 891
FblI GTMKAC 1 cut(s) 295
Fnu4HI GCNGC 7 cut(s) 355, 408, 673, 859, 976, 1318, 1458
FokI GGATG 4 cut(s) 166, 377, 688, 739
Fsp4HI GCNGC 7 cut(s) 355, 408, 673, 859, 976, 1318, 1458
FspBI CTAG 1 cut(s) 65
GlaI GCGC 3 cut(s) 1084, 1321, 1378
GluI GCNGC 7 cut(s) 355, 408, 673, 859, 976, 1318, 1458
GsuI CTGGAG 1 cut(s) 778
HaeII RGCGCY 3 cut(s) 1086, 1323, 1380
HaeIII GGCC 5 cut(s) 407, 492, 978, 984, 1103
HapII CCGG 4 cut(s) 285, 834, 912, 1227
HgaI GACGC 1 cut(s) 1130
HhaI GCGC 3 cut(s) 1085, 1322, 1379
Hin1I GRCGYC 3 cut(s) 255, 1083, 1377
Hin6I GCGC 3 cut(s) 1083, 1320, 1377
HinP1I GCGC 3 cut(s) 1083, 1320, 1377
HincII GTYRAC 3 cut(s) 296, 604, 961
HindII GTYRAC 3 cut(s) 296, 604, 961
HinfI GANTC 3 cut(s) 281, 872, 1089
HpaII CCGG 4 cut(s) 285, 834, 912, 1227
HphI GGTGA 4 cut(s) 553, 778, 946, 1078
Hpy166II GTNNAC 7 cut(s) 188, 209, 296, 604, 820, 961, 1069
Hpy188I TCNGA 7 cut(s) 172, 219, 612, 877, 1212, 1268, 1411
Hpy188III TCNNGA 3 cut(s) 23, 285, 578
Hpy8I GTNNAC 7 cut(s) 188, 209, 296, 604, 820, 961, 1069
Hpy99I CGWCG 5 cut(s) 219, 362, 629, 857, 1291
HpyAV CCTTC 1 cut(s) 274
HpyCH4III ACNGT 6 cut(s) 109, 608, 790, 815, 958, 1048
HpyCH4IV ACGT 6 cut(s) 214, 255, 398, 590, 966, 1296
HpyF10VI GCNNNNNNNGC 4 cut(s) 269, 981, 1319, 1463
HpyF3I CTNAG 5 cut(s) 13, 92, 148, 273, 1437
HpySE526I ACGT 6 cut(s) 214, 255, 398, 590, 966, 1296
Hsp92I GRCGYC 3 cut(s) 255, 1083, 1377
HspAI GCGC 3 cut(s) 1083, 1320, 1377
KasI GGCGCC 2 cut(s) 1082, 1376
Kpn2I TCCGGA 1 cut(s) 284
Ksp22I TGATCA 1 cut(s) 891
Kzo9I GATC 9 cut(s) 95, 325, 585, 612, 747, 891, 1183, 1207, 1356
LmnI GCTCC 6 cut(s) 219, 509, 511, 835, 1156, 1254
Lsp1109I GCAGC 4 cut(s) 659, 845, 1329, 1469
LweI GCATC 5 cut(s) 118, 188, 909, 999, 1415
MaeI CTAG 1 cut(s) 65
MaeII ACGT 6 cut(s) 214, 255, 398, 590, 966, 1296
MaeIII GTNAC 9 cut(s) 256, 616, 802, 967, 1042, 1048, 1305, 1415, 1429
MalI GATC 9 cut(s) 97, 327, 587, 614, 749, 893, 1185, 1209, 1358
MboI GATC 9 cut(s) 95, 325, 585, 612, 747, 891, 1183, 1207, 1356
MboII GAAGA 2 cut(s) 88, 757
MflI RGATCY 2 cut(s) 325, 1207
MhlI GDGCHC 5 cut(s) 211, 461, 822, 946, 1283
MlsI TGGCCA 1 cut(s) 984
MluCI AATT 4 cut(s) 54, 528, 713, 1131
MluNI TGGCCA 1 cut(s) 984
Mly113I GGCGCC 2 cut(s) 1083, 1377
MmeI TCCRAC 5 cut(s) 197, 353, 729, 900, 979
MnlI CCTC 9 cut(s) 233, 410, 532, 583, 1109, 1157, 1294, 1330, 1367
Mox20I TGGCCA 1 cut(s) 984
MroI TCCGGA 1 cut(s) 284
MscI TGGCCA 1 cut(s) 984
MseI TTAA 5 cut(s) 57, 393, 726, 885, 1275
MslI CAYNNNNRTG 3 cut(s) 867, 1312, 1411
Msp20I TGGCCA 1 cut(s) 984
MspI CCGG 4 cut(s) 285, 834, 912, 1227
MspR9I CCNGG 3 cut(s) 315, 834, 981
Mva1269I GAATGC 2 cut(s) 386, 736
MvaI CCWGG 2 cut(s) 315, 981
MwoI GCNNNNNNNGC 4 cut(s) 269, 981, 1319, 1463
NarI GGCGCC 2 cut(s) 1083, 1377
NciI CCSGG 1 cut(s) 834
NcoI CCATGG 1 cut(s) 985
NdeII GATC 9 cut(s) 95, 325, 585, 612, 747, 891, 1183, 1207, 1356
NlaIV GGNNCC 9 cut(s) 221, 432, 513, 910, 943, 1084, 1198, 1282, 1378
NmuCI GTSAC 4 cut(s) 256, 616, 1048, 1305
NspI RCATGY 4 cut(s) 169, 414, 1163, 1317
NspV TTCGAA 1 cut(s) 594
OliI CACNNNNGTG 1 cut(s) 867
PaeI GCATGC 2 cut(s) 414, 1317
PcsI WCGNNNNNNNCGW 4 cut(s) 612, 621, 1029, 1146
PctI GAATGC 2 cut(s) 386, 736
PfeI GAWTC 3 cut(s) 281, 872, 1089
PkrI GCNGC 7 cut(s) 356, 409, 674, 860, 977, 1319, 1459
PluTI GGCGCC 2 cut(s) 1086, 1380
Psp1406I AACGTT 2 cut(s) 966, 1296
Psp6I CCWGG 2 cut(s) 313, 979
PspGI CCWGG 2 cut(s) 313, 979
PspN4I GGNNCC 9 cut(s) 221, 432, 513, 910, 943, 1084, 1198, 1282, 1378
PspPI GGNCC 5 cut(s) 431, 473, 491, 977, 1101
PsrI GAACNNNNNNTAC 2 cut(s) 752, 784
PsuI RGATCY 2 cut(s) 325, 1207
RsaI GTAC 3 cut(s) 69, 425, 770
RsaNI GTAC 3 cut(s) 68, 424, 769
RseI CAYNNNNRTG 3 cut(s) 867, 1312, 1411
SalI GTCGAC 1 cut(s) 294
SaqAI TTAA 5 cut(s) 57, 393, 726, 885, 1275
SatI GCNGC 7 cut(s) 355, 408, 673, 859, 976, 1318, 1458
Sau3AI GATC 9 cut(s) 95, 325, 585, 612, 747, 891, 1183, 1207, 1356
Sau96I GGNCC 5 cut(s) 431, 473, 491, 977, 1101
ScaI AGTACT 1 cut(s) 770
ScrFI CCNGG 3 cut(s) 315, 834, 981
SduI GDGCHC 5 cut(s) 211, 461, 822, 946, 1283
SfaNI GCATC 5 cut(s) 118, 188, 909, 999, 1415
SfoI GGCGCC 2 cut(s) 1084, 1378
SfuI TTCGAA 1 cut(s) 594
SinI GGWCC 2 cut(s) 431, 473
SmiMI CAYNNNNRTG 3 cut(s) 867, 1312, 1411
SphI GCATGC 2 cut(s) 414, 1317
Sse9I AATT 4 cut(s) 54, 528, 713, 1131
SsiI CCGC 6 cut(s) 318, 354, 408, 946, 975, 1128
SspDI GGCGCC 2 cut(s) 1082, 1376
SspMI CTAG 1 cut(s) 65
StyD4I CCNGG 3 cut(s) 313, 832, 979
StyI CCWWGG 3 cut(s) 487, 507, 985
TaaI ACNGT 6 cut(s) 109, 608, 790, 815, 958, 1048
TaiI ACGT 6 cut(s) 217, 258, 401, 593, 969, 1299
TaqI TCGA 5 cut(s) 295, 594, 1140, 1182, 1359
TasI AATT 4 cut(s) 54, 528, 713, 1131
TatI WGTACW 3 cut(s) 67, 423, 768
TauI GCSGC 3 cut(s) 357, 410, 978
TfiI GAWTC 3 cut(s) 281, 872, 1089
Tru1I TTAA 5 cut(s) 57, 393, 726, 885, 1275
Tru9I TTAA 5 cut(s) 57, 393, 726, 885, 1275
TscAI CASTG 6 cut(s) 232, 820, 832, 874, 1000, 1314
TseFI GTSAC 4 cut(s) 256, 616, 1048, 1305
TseI GCWGC 4 cut(s) 672, 858, 1317, 1457
Tsp45I GTSAC 4 cut(s) 256, 616, 1048, 1305
TspDTI ATGAA 5 cut(s) 317, 379, 650, 706, 725
TspGWI ACGGA 5 cut(s) 642, 678, 1128, 1386, 1412
TspRI CASTG 6 cut(s) 232, 820, 832, 874, 1000, 1314
VneI GTGCAC 2 cut(s) 207, 818
VpaK11BI GGWCC 2 cut(s) 431, 473
XapI RAATTY 2 cut(s) 528, 713
XceI RCATGY 4 cut(s) 169, 414, 1163, 1317
XcmI CCANNNNNNNNNTGG 1 cut(s) 1060
XmiI GTMKAC 1 cut(s) 295
XspI CTAG 1 cut(s) 65
ZraI GACGTC 1 cut(s) 256
ZrmI AGTACT 1 cut(s) 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.