pycom11g26850

Exopolygalacturonase clone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0016557
Physical Location & Seq
Reverse (-)
29304879 .. 29306009
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g26850.2

Sequence Viewer

Length: 888 bp
ATGCAGTCACTTAAATTGAACAAAGTAAACAACACCCTGATCGAAGGGATCACATCCTTAAACAGCAAATTCTTCCACATACACATGTTCGGCTGCAAAAATGTGACAATGAGCAATCTCCACCTCATTGCTCCTGGAGATAGCCCTAACACAGATGGCCTGCACATCAGCACTTCGAGCCAAATCAAAGTGCTTAACAGCGTTATGGCAACTGGCGATGACTGCGTTTCCATCGGCCAAGGCTCTCATGATATCACCGTTAAGAACGTCACCTGTGGTCCAGGACATGGCATCAGCATCGGGAGCCTCGGAAAGTACGCCGACGAACTGAGCGTCAGTCAGATTCTCGTGAGCAACTGCACATTGAGAAACACCACCAACGGTGCCAGAATCAAGACTTGGGCTGGCGAAAGTGCAGGAGAAGCGTCCGGAATCATCTACGAAGACATTATCATGGACCAAGTTCAAAACCCTGTTGTCATAGATCAGAACTACGGTGACAAGAAGAAGGTAAAGAAAAACATCTTTGGAAATAAAAAGATGGTAGTGTCAGGAAATAAAAAGAGGGTAAGTGCAGGATTAGAAAAGAAGGTAGGAGCAGGATTAAAAAAGCAGGTACGCATGCATGAAAGAGCTGGACCACCTTCTGGACCAGCATCAAAGTGGAAGATCAGTGACGTTCACTTCAGAAACATTAGGGGCACCTCAGCCAGAAACGTAGCCGTTTCGTTAGCTTGCAGCTCTGCAAACCCATGCGAGGGTGTTGAGTTGACCGACATTAACTTTTCCTACCAGGGCACCAGTATGAAAAGCACGGCGCTTACATCTGAGTGTTTCAACGCCAAGATTACATCAAAGGGCGTACAGAACCCACCACTCTGTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0016787 GO:0016798 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

296

Amino Acids

31.55

Weight (kDa)

9.42

Isoelectric Point (pI)

31.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016557)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g26010
malus_domestica MD03G1291400.v1.1 MD11G1306900.v1.1
prunus_persica Prupe.8G264500_v2.0.a1
pyrus_communis pycom03g23080 pycom11g26850
rosa_chinensis RchiOBHm_Chr6g0294381
rosa_laevigata RLG00000011876
rosa_multiflora Rmu_sc0005369.1_g000014
rosa_roxburghii Rroxscaffold_7G00173430
rosa_rugosa Rorug06G0243900
rosa_samantha Rh6AG356500 Rh6CG370500
rosa_wichuraiana Rw6G031070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 604
AccB1I GGYRCC 3 cut(s) 383, 701, 797
AccB7I CCANNNNNTGG 2 cut(s) 287, 647
AccIII TCCGGA 1 cut(s) 428
AclWI GGATC 1 cut(s) 56
AcoI YGGCCR 1 cut(s) 235
AcsI RAATTY 1 cut(s) 68
AcuI CTGAAG 1 cut(s) 670
AfaI GTAC 3 cut(s) 317, 618, 864
AfiI CCNNNNNNNGG 4 cut(s) 287, 647, 757, 758
AflIII ACRYGT 1 cut(s) 84
AgsI TTSAA 3 cut(s) 19, 467, 838
AjnI CCWGG 3 cut(s) 133, 280, 792
AjuI GAANNNNNNNTTGG 2 cut(s) 382, 414
AluBI AGCT 3 cut(s) 635, 734, 741
AluI AGCT 3 cut(s) 635, 734, 741
AlwI GGATC 1 cut(s) 56
Aor13HI TCCGGA 1 cut(s) 428
AoxI GGCC 2 cut(s) 157, 235
ApeKI GCWGC 2 cut(s) 93, 738
ApoI RAATTY 1 cut(s) 68
ArsI GACNNNNNNTTYG 2 cut(s) 318, 350
AspLEI GCGC 1 cut(s) 820
AspS9I GGNCC 4 cut(s) 278, 457, 638, 650
AsuHPI GGTGA 3 cut(s) 247, 262, 509
AvaII GGWCC 4 cut(s) 278, 457, 638, 650
BaeGI GKGCMC 2 cut(s) 704, 800
BanI GGYRCC 3 cut(s) 383, 701, 797
BauI CACGAG 1 cut(s) 347
BbsI GAAGAC 1 cut(s) 450
BbvCI CCTCAGC 1 cut(s) 706
BbvI GCAGC 2 cut(s) 80, 750
BccI CCATC 3 cut(s) 149, 239, 535
BceAI ACGGC 2 cut(s) 707, 831
BcgI CGANNNNNNTGC 2 cut(s) 280, 314
BciT130I CCWGG 3 cut(s) 135, 282, 794
BfoI RGCGCY 1 cut(s) 821
BfuAI ACCTGC 1 cut(s) 604
BisI GCNGC 2 cut(s) 94, 739
BlsI GCNGC 2 cut(s) 95, 740
Bme1390I CCNGG 3 cut(s) 135, 282, 794
Bme18I GGWCC 4 cut(s) 278, 457, 638, 650
BmgT120I GGNCC 4 cut(s) 278, 457, 638, 650
BmiI GGNNCC 4 cut(s) 305, 385, 703, 799
BmrFI CCNGG 3 cut(s) 135, 282, 794
BmsI GCATC 3 cut(s) 300, 306, 665
BpiI GAAGAC 1 cut(s) 450
BpmI CTGGAG 1 cut(s) 156
Bpu10I CCTNAGC 1 cut(s) 706
BsaJI CCNNGG 3 cut(s) 238, 307, 793
BsaWI WCCGGW 1 cut(s) 428
Bsc4I CCNNNNNNNGG 4 cut(s) 287, 647, 757, 758
Bse1I ACTGG 2 cut(s) 217, 801
Bse3DI GCAATG 1 cut(s) 126
BseAI TCCGGA 1 cut(s) 428
BseBI CCWGG 3 cut(s) 135, 282, 794
BseDI CCNNGG 3 cut(s) 238, 307, 793
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 4 cut(s) 287, 647, 757, 758
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 3 cut(s) 320, 720, 819
BseNI ACTGG 2 cut(s) 217, 801
BseSI GKGCMC 2 cut(s) 704, 800
BseXI GCAGC 2 cut(s) 80, 750
BsgI GTGCAG 4 cut(s) 146, 343, 435, 594
BshFI GGCC 2 cut(s) 159, 237
BshNI GGYRCC 3 cut(s) 383, 701, 797
BsiSI CCGG 1 cut(s) 429
BslI CCNNNNNNNGG 4 cut(s) 287, 647, 757, 758
BsnI GGCC 2 cut(s) 159, 237
Bsp1286I GDGCHC 2 cut(s) 704, 800
Bsp13I TCCGGA 1 cut(s) 428
Bsp143I GATC 4 cut(s) 39, 48, 484, 669
BspANI GGCC 2 cut(s) 159, 237
BspCNI CTCAG 3 cut(s) 321, 719, 820
BspEI TCCGGA 1 cut(s) 428
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 4 cut(s) 305, 385, 703, 799
BspMI ACCTGC 1 cut(s) 604
BspPI GGATC 1 cut(s) 56
BspT107I GGYRCC 3 cut(s) 383, 701, 797
BsrDI GCAATG 1 cut(s) 126
BsrI ACTGG 2 cut(s) 217, 801
BssECI CCNNGG 3 cut(s) 238, 307, 793
BssMI GATC 4 cut(s) 39, 48, 484, 669
BssSI CACGAG 1 cut(s) 347
BssT1I CCWWGG 1 cut(s) 238
Bst2BI CACGAG 1 cut(s) 347
Bst2UI CCWGG 3 cut(s) 135, 282, 794
Bst4CI ACNGT 3 cut(s) 259, 383, 497
BstC8I GCNNGC 4 cut(s) 161, 406, 623, 736
BstDEI CTNAG 3 cut(s) 329, 706, 828
BstF5I GGATG 1 cut(s) 53
BstH2I RGCGCY 1 cut(s) 821
BstHHI GCGC 1 cut(s) 820
BstKTI GATC 4 cut(s) 42, 51, 487, 672
BstMBI GATC 4 cut(s) 39, 48, 484, 669
BstMWI GCNNNNNNNGC 4 cut(s) 177, 222, 303, 422
BstNI CCWGG 3 cut(s) 135, 282, 794
BstNSI RCATGY 2 cut(s) 88, 625
BstSCI CCNGG 3 cut(s) 133, 280, 792
BstSLI GKGCMC 2 cut(s) 704, 800
BstV1I GCAGC 2 cut(s) 80, 750
BstV2I GAAGAC 1 cut(s) 450
BsuRI GGCC 2 cut(s) 159, 237
BtgZI GCGATG 1 cut(s) 231
BtsCI GGATG 1 cut(s) 53
BtsIMutI CAGTG 1 cut(s) 679
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 4 cut(s) 161, 406, 623, 736
CciI TCATGA 1 cut(s) 247
CfoI GCGC 1 cut(s) 820
Cfr13I GGNCC 4 cut(s) 278, 457, 638, 650
CseI GACGC 2 cut(s) 322, 414
Csp6I GTAC 3 cut(s) 316, 617, 863
CviAII CATG 7 cut(s) 85, 248, 287, 454, 622, 626, 753
CviQI GTAC 3 cut(s) 316, 617, 863
DdeI CTNAG 3 cut(s) 329, 706, 828
DpnI GATC 4 cut(s) 41, 50, 486, 671
DpnII GATC 4 cut(s) 39, 48, 484, 669
EaeI YGGCCR 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 238
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 4 cut(s) 278, 457, 638, 650
Eco57I CTGAAG 1 cut(s) 670
EcoRII CCWGG 3 cut(s) 133, 280, 792
EcoRV GATATC 1 cut(s) 253
EcoT14I CCWWGG 1 cut(s) 238
EcoT22I ATGCAT 1 cut(s) 627
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 7 cut(s) 88, 251, 290, 457, 625, 629, 756
FatI CATG 7 cut(s) 84, 247, 286, 453, 621, 625, 752
Fnu4HI GCNGC 2 cut(s) 94, 739
FokI GGATG 1 cut(s) 40
Fsp4HI GCNGC 2 cut(s) 94, 739
GlaI GCGC 1 cut(s) 819
GluI GCNGC 2 cut(s) 94, 739
GsuI CTGGAG 1 cut(s) 156
HaeII RGCGCY 1 cut(s) 821
HaeIII GGCC 2 cut(s) 159, 237
HapII CCGG 1 cut(s) 429
HgaI GACGC 2 cut(s) 322, 414
HhaI GCGC 1 cut(s) 820
Hin1II CATG 7 cut(s) 88, 251, 290, 457, 625, 629, 756
Hin6I GCGC 1 cut(s) 818
HinP1I GCGC 1 cut(s) 818
HincII GTYRAC 1 cut(s) 771
HindII GTYRAC 1 cut(s) 771
HinfI GANTC 3 cut(s) 343, 390, 432
HpaII CCGG 1 cut(s) 429
HphI GGTGA 3 cut(s) 247, 262, 509
Hpy166II GTNNAC 3 cut(s) 28, 682, 771
Hpy188I TCNGA 5 cut(s) 311, 342, 489, 689, 829
Hpy188III TCNNGA 7 cut(s) 248, 301, 349, 394, 429, 552, 648
Hpy8I GTNNAC 3 cut(s) 28, 682, 771
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 4 cut(s) 38, 502, 583, 654
HpyCH4III ACNGT 3 cut(s) 259, 383, 497
HpyCH4IV ACGT 3 cut(s) 267, 678, 717
HpyCH4V TGCA 9 cut(s) 4, 96, 163, 360, 416, 575, 625, 738, 746
HpyF10VI GCNNNNNNNGC 4 cut(s) 177, 222, 303, 422
HpyF3I CTNAG 3 cut(s) 329, 706, 828
HpySE526I ACGT 3 cut(s) 267, 678, 717
Hsp92II CATG 7 cut(s) 88, 251, 290, 457, 625, 629, 756
HspAI GCGC 1 cut(s) 818
Kpn2I TCCGGA 1 cut(s) 428
Kzo9I GATC 4 cut(s) 39, 48, 484, 669
LmnI GCTCC 3 cut(s) 136, 303, 596
Lsp1109I GCAGC 2 cut(s) 80, 750
LweI GCATC 3 cut(s) 300, 306, 665
MaeII ACGT 3 cut(s) 267, 678, 717
MaeIII GTNAC 5 cut(s) 6, 103, 268, 497, 674
MalI GATC 4 cut(s) 41, 50, 486, 671
MboI GATC 4 cut(s) 39, 48, 484, 669
MboII GAAGA 4 cut(s) 64, 455, 517, 679
MhlI GDGCHC 2 cut(s) 704, 800
MluCI AATT 2 cut(s) 14, 68
MnlI CCTC 5 cut(s) 134, 317, 558, 715, 751
Mph1103I ATGCAT 1 cut(s) 627
MroI TCCGGA 1 cut(s) 428
MseI TTAA 7 cut(s) 12, 59, 195, 261, 605, 780, 886
MslI CAYNNNNRTG 5 cut(s) 83, 452, 661, 803, 829
MspI CCGG 1 cut(s) 429
MspR9I CCNGG 3 cut(s) 135, 282, 794
MvaI CCWGG 3 cut(s) 135, 282, 794
MwoI GCNNNNNNNGC 4 cut(s) 177, 222, 303, 422
NdeII GATC 4 cut(s) 39, 48, 484, 669
NlaIII CATG 7 cut(s) 88, 251, 290, 457, 625, 629, 756
NlaIV GGNNCC 4 cut(s) 305, 385, 703, 799
NmuCI GTSAC 5 cut(s) 6, 103, 268, 497, 674
NsiI ATGCAT 1 cut(s) 627
NspI RCATGY 2 cut(s) 88, 625
PaeI GCATGC 1 cut(s) 625
PagI TCATGA 1 cut(s) 247
PciI ACATGT 1 cut(s) 84
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PfeI GAWTC 3 cut(s) 343, 390, 432
PflMI CCANNNNNTGG 2 cut(s) 287, 647
PfoI TCCNGGA 2 cut(s) 133, 280
PkrI GCNGC 2 cut(s) 95, 740
PscI ACATGT 1 cut(s) 84
Psp6I CCWGG 3 cut(s) 133, 280, 792
PspGI CCWGG 3 cut(s) 133, 280, 792
PspN4I GGNNCC 4 cut(s) 305, 385, 703, 799
PspPI GGNCC 4 cut(s) 278, 457, 638, 650
RsaI GTAC 3 cut(s) 317, 618, 864
RsaNI GTAC 3 cut(s) 316, 617, 863
RseI CAYNNNNRTG 5 cut(s) 83, 452, 661, 803, 829
SaqAI TTAA 7 cut(s) 12, 59, 195, 261, 605, 780, 886
SatI GCNGC 2 cut(s) 94, 739
Sau3AI GATC 4 cut(s) 39, 48, 484, 669
Sau96I GGNCC 4 cut(s) 278, 457, 638, 650
ScrFI CCNGG 3 cut(s) 135, 282, 794
SduI GDGCHC 2 cut(s) 704, 800
SfaNI GCATC 3 cut(s) 300, 306, 665
SinI GGWCC 4 cut(s) 278, 457, 638, 650
SmiMI CAYNNNNRTG 5 cut(s) 83, 452, 661, 803, 829
SphI GCATGC 1 cut(s) 625
Sse9I AATT 2 cut(s) 14, 68
StyD4I CCNGG 3 cut(s) 133, 280, 792
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 3 cut(s) 259, 383, 497
TaiI ACGT 3 cut(s) 270, 681, 720
TaqI TCGA 2 cut(s) 42, 176
TaqII GACCGA 1 cut(s) 788
TasI AATT 2 cut(s) 14, 68
TfiI GAWTC 3 cut(s) 343, 390, 432
Tru1I TTAA 7 cut(s) 12, 59, 195, 261, 605, 780, 886
Tru9I TTAA 7 cut(s) 12, 59, 195, 261, 605, 780, 886
TscAI CASTG 1 cut(s) 679
TseFI GTSAC 5 cut(s) 6, 103, 268, 497, 674
TseI GCWGC 2 cut(s) 93, 738
Tsp45I GTSAC 5 cut(s) 6, 103, 268, 497, 674
TspDTI ATGAA 2 cut(s) 642, 821
TspRI CASTG 1 cut(s) 679
Van91I CCANNNNNTGG 2 cut(s) 287, 647
VpaK11BI GGWCC 4 cut(s) 278, 457, 638, 650
XapI RAATTY 1 cut(s) 68
XceI RCATGY 2 cut(s) 88, 625
XcmI CCANNNNNNNNNTGG 1 cut(s) 660
Zsp2I ATGCAT 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.