Rroxscaffold_7G00173430

Exopolygalacturonase clone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0016557
Physical Location & Seq
Reverse (-)
13422302 .. 13424261
1960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00173430.1

Sequence Viewer

Length: 1419 bp
ATGGACACCGCAAGGAGATTGGAGGCTATTCTTGTCGTAGGCTTAGTTTTTCTCGCTTGCGCGGCCAATGGTGCATCCCGTGGACATCTTTTTGGGGTTGATGCACCTCGTCGGAGCCTCACGGATACTACGAACCGAAAAGTATTTGACGTCACAGCCTTCGGTGCTAAGCCTGAAGCCGGCGATTCAAAAGGACGCAAAATGAGAGAGCTTCCCGGCGGTGGAGATATAGACGCTCCTCTACCACAACTTCCCGTTGCTGGTGGCGGCGACTCCGGCAGCGATGATGGGAATGATGACGGTTCGAATCCCGATGCTGACAATGATACTGGAATGGCATTCATTAAGACGTGGGTGGCCGCATGCAGGGGAGAGTACAAGGGTCCAGCAAAGGTTCTTATTCCAAAGGGCACATTTTTGGCTGGACCTGTTGTGTTCCAAGGGCCATGCACCAGCTCCAAGGAGCCAATCGTTTTGGAAATTCAGGGAGAAGTGAAAGCCACCACCGATTTGAGTGATTATTCCTCTCCTGAATGGATCTCGTTCGAAAATATTGACGGTTTGATTGTCACCGGTGATGGAGTTATCAATGGCCAAGGCGAGAAAACATGGAACGGTGCCGGCTGCCATAGCGACGACTGCCCTCATTCTCCAACCAACATAAAATTCCAGAAGGTTAAGAATGCACTTATCGAGGGGATCACATCCTTGAACAGCAAGTACTTTCATTACCACATCACCGACTCCAGCAACGTTACCATGAACAATGTGCACATTACTGCCCCTGGCGATAGCCCTAACACAGACGGCTGCCACATCAGTAGTTCCGACAACGTCAAAGTGATCAACAGCATCATTGGAACCGGGGACGACTGCGTTTCCGTCGGCCAGGGCGCGCGCCAAGTTACCATCAACAACGTTACTTGTGGCCCTGGCCATGGCATCAGTGTTGGAAGTCTTGGTAAGTACAAGGACGAACAACCCGTGGTCGGTGTCACCGTGACCAATTGCACATTGTCGAACACAACCAACGGCGCCAGAATCAAAACTTGGGCCGGGCCGAACAGAGGAGAAGCAAGAGGAATTATATTCGACAACCTCGTCTTGGACCATGTGAAAGCCCCAATAGTCATCGATCAACACTACGGAGAAAAGAAAAGTGGGGGAGACAATAAAAATGCGAAGGATCCAAACTCCGGAAAGCAATCCCTATATAAGCTTAGTGATATTCACTTCAGAAACATTAAGGGCACCTCGTCGCACAACGTTGCCGTGTCACTATCATGCAGCGCTACAAACCCTTGCGATGGGGTTGAATTGAATAACATCGACATTGCCTTAGGAGGAGCCGGCACCGCCAAAAAACAGACGTTGCATCGGAATGTTACAACGCAAAGATTACCACTAAGGGCACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0016787 GO:0016798 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

472

Amino Acids

49.64

Weight (kDa)

6.23

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 129 - 457 3.7e-87 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016557)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g26010
malus_domestica MD03G1291400.v1.1 MD11G1306900.v1.1
prunus_persica Prupe.8G264500_v2.0.a1
pyrus_communis pycom03g23080 pycom11g26850
rosa_chinensis RchiOBHm_Chr6g0294381
rosa_laevigata RLG00000011876
rosa_multiflora Rmu_sc0005369.1_g000014
rosa_roxburghii Rroxscaffold_7G00173430
rosa_rugosa Rorug06G0243900
rosa_samantha Rh6AG356500 Rh6CG370500
rosa_wichuraiana Rw6G031070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1100
AatII GACGTC 1 cut(s) 153
AccB1I GGYRCC 4 cut(s) 617, 1034, 1250, 1352
AccII CGCG 3 cut(s) 62, 896, 898
AccIII TCCGGA 1 cut(s) 1196
AciI CCGC 6 cut(s) 9, 62, 219, 267, 360, 1356
AclI AACGTT 3 cut(s) 753, 918, 1266
AclWI GGATC 4 cut(s) 545, 707, 1181, 1194
AcoI YGGCCR 5 cut(s) 63, 357, 592, 886, 934
AcsI RAATTY 2 cut(s) 480, 665
AcuI CTGAAG 2 cut(s) 195, 1219
AcyI GRCGYC 2 cut(s) 150, 1035
AfaI GTAC 3 cut(s) 377, 722, 968
AfeI AGCGCT 1 cut(s) 1291
AgeI ACCGGT 1 cut(s) 572
AgsI TTSAA 4 cut(s) 189, 712, 1316, 1321
AjiI CACGTC 1 cut(s) 351
AjnI CCWGG 3 cut(s) 784, 888, 931
AjuI GAANNNNNNNTTGG 4 cut(s) 650, 682, 1033, 1065
AluBI AGCT 3 cut(s) 211, 456, 1219
AluI AGCT 3 cut(s) 211, 456, 1219
Alw21I GWGCWC 1 cut(s) 774
Alw26I GTCTC 1 cut(s) 1161
Alw44I GTGCAC 1 cut(s) 770
AlwI GGATC 4 cut(s) 545, 707, 1181, 1194
Aor13HI TCCGGA 1 cut(s) 1196
Aor51HI AGCGCT 1 cut(s) 1291
AoxI GGCC 9 cut(s) 63, 357, 443, 592, 886, 928, 934, 1053, 1058
ApaLI GTGCAC 1 cut(s) 770
ApeKI GCWGC 4 cut(s) 279, 624, 810, 1287
ApoI RAATTY 2 cut(s) 480, 665
AsiGI ACCGGT 1 cut(s) 572
AspLEI GCGC 6 cut(s) 62, 896, 898, 900, 1037, 1292
AspS9I GGNCC 7 cut(s) 383, 425, 443, 929, 1053, 1058, 1108
AsuC2I CCSGG 3 cut(s) 216, 865, 1057
AsuHPI GGTGA 4 cut(s) 562, 587, 730, 988
AsuII TTCGAA 2 cut(s) 305, 546
AvaII GGWCC 3 cut(s) 383, 425, 1108
AxyI CCTNAGG 1 cut(s) 1339
BaeGI GKGCMC 4 cut(s) 413, 774, 1253, 1414
BalI TGGCCA 2 cut(s) 594, 936
BamHI GGATCC 1 cut(s) 1186
BanI GGYRCC 4 cut(s) 617, 1034, 1250, 1352
Bbv12I GWGCWC 1 cut(s) 774
BbvI GCAGC 4 cut(s) 291, 611, 797, 1299
BccI CCATC 4 cut(s) 281, 572, 917, 1301
BceAI ACGGC 3 cut(s) 823, 1048, 1256
BciT130I CCWGG 3 cut(s) 786, 890, 933
BciVI GTATCC 1 cut(s) 118
BclI TGATCA 1 cut(s) 843
BcnI CCSGG 3 cut(s) 216, 865, 1057
BcoDI GTCTC 1 cut(s) 1161
BfoI RGCGCY 2 cut(s) 1038, 1293
BfuI GTATCC 1 cut(s) 118
BisI GCNGC 7 cut(s) 63, 268, 280, 360, 625, 811, 1288
BlpI GCTNAGC 1 cut(s) 168
BlsI GCNGC 7 cut(s) 64, 269, 281, 361, 626, 812, 1289
BmcAI AGTACT 1 cut(s) 722
Bme1390I CCNGG 6 cut(s) 216, 786, 865, 890, 933, 1057
Bme18I GGWCC 3 cut(s) 383, 425, 1108
BmgBI CACGTC 1 cut(s) 351
BmgT120I GGNCC 7 cut(s) 383, 425, 443, 929, 1053, 1058, 1108
BmrFI CCNGG 6 cut(s) 216, 786, 865, 890, 933, 1057
BmsI GCATC 6 cut(s) 83, 91, 304, 861, 951, 1384
BpmI CTGGAG 1 cut(s) 730
Bpu1102I GCTNAGC 1 cut(s) 168
Bpu14I TTCGAA 2 cut(s) 305, 546
BpuMI CCSGG 3 cut(s) 216, 865, 1057
Bsa29I ATCGAT 1 cut(s) 1134
BsaHI GRCGYC 2 cut(s) 150, 1035
BsaWI WCCGGW 2 cut(s) 572, 1196
Bse118I RCCGGY 4 cut(s) 179, 572, 620, 1349
Bse1I ACTGG 1 cut(s) 334
Bse21I CCTNAGG 1 cut(s) 1339
Bse3DI GCAATG 1 cut(s) 1332
BseAI TCCGGA 1 cut(s) 1196
BseBI CCWGG 3 cut(s) 786, 890, 933
BseCI ATCGAT 1 cut(s) 1134
BseGI GGATG 2 cut(s) 74, 704
BseMI GCAATG 1 cut(s) 1332
BseNI ACTGG 1 cut(s) 334
BsePI GCGCGC 2 cut(s) 894, 896
BseRI GAGGAG 3 cut(s) 228, 1083, 1359
BseSI GKGCMC 4 cut(s) 413, 774, 1253, 1414
BseXI GCAGC 4 cut(s) 291, 611, 797, 1299
Bsh1236I CGCG 3 cut(s) 62, 896, 898
BshFI GGCC 9 cut(s) 65, 359, 445, 594, 888, 930, 936, 1055, 1060
BshNI GGYRCC 4 cut(s) 617, 1034, 1250, 1352
BshTI ACCGGT 1 cut(s) 572
BshVI ATCGAT 1 cut(s) 1134
BsiHKAI GWGCWC 1 cut(s) 774
BsiSI CCGG 9 cut(s) 180, 216, 276, 573, 621, 864, 1056, 1197, 1350
BslFI GGGAC 1 cut(s) 881
BsmAI GTCTC 1 cut(s) 1161
BsmFI GGGAC 1 cut(s) 881
BsmI GAATGC 2 cut(s) 338, 688
BsnI GGCC 9 cut(s) 65, 359, 445, 594, 888, 930, 936, 1055, 1060
Bsp119I TTCGAA 2 cut(s) 305, 546
Bsp1286I GDGCHC 4 cut(s) 413, 774, 1253, 1414
Bsp13I TCCGGA 1 cut(s) 1196
Bsp143I GATC 5 cut(s) 537, 699, 843, 1135, 1186
Bsp1720I GCTNAGC 1 cut(s) 168
Bsp19I CCATGG 1 cut(s) 937
BspACI CCGC 6 cut(s) 9, 62, 219, 267, 360, 1356
BspANI GGCC 9 cut(s) 65, 359, 445, 594, 888, 930, 936, 1055, 1060
BspDI ATCGAT 1 cut(s) 1134
BspEI TCCGGA 1 cut(s) 1196
BspFNI CGCG 3 cut(s) 62, 896, 898
BspPI GGATC 4 cut(s) 545, 707, 1181, 1194
BspT104I TTCGAA 2 cut(s) 305, 546
BspT107I GGYRCC 4 cut(s) 617, 1034, 1250, 1352
BsrDI GCAATG 1 cut(s) 1332
BsrFI RCCGGY 4 cut(s) 179, 572, 620, 1349
BsrI ACTGG 1 cut(s) 334
BssAI RCCGGY 4 cut(s) 179, 572, 620, 1349
BssHII GCGCGC 2 cut(s) 894, 896
BssMI GATC 5 cut(s) 537, 699, 843, 1135, 1186
BssNI GRCGYC 2 cut(s) 150, 1035
BssT1I CCWWGG 4 cut(s) 439, 459, 595, 937
Bst2UI CCWGG 3 cut(s) 786, 890, 933
Bst4CI ACNGT 4 cut(s) 302, 560, 617, 1000
BstACI GRCGYC 2 cut(s) 150, 1035
BstBI TTCGAA 2 cut(s) 305, 546
BstC8I GCNNGC 7 cut(s) 58, 181, 364, 622, 896, 898, 1351
BstDEI CTNAG 5 cut(s) 43, 168, 1220, 1339, 1406
BstDSI CCRYGG 3 cut(s) 79, 937, 984
BstF5I GGATG 2 cut(s) 74, 704
BstFNI CGCG 3 cut(s) 62, 896, 898
BstH2I RGCGCY 2 cut(s) 1038, 1293
BstHHI GCGC 6 cut(s) 62, 896, 898, 900, 1037, 1292
BstKTI GATC 5 cut(s) 540, 702, 846, 1138, 1189
BstMAI GTCTC 1 cut(s) 1161
BstMBI GATC 5 cut(s) 537, 699, 843, 1135, 1186
BstMWI GCNNNNNNNGC 7 cut(s) 62, 71, 164, 276, 630, 639, 1355
BstNI CCWGG 3 cut(s) 786, 890, 933
BstNSI RCATGY 1 cut(s) 366
BstSCI CCNGG 6 cut(s) 214, 784, 863, 888, 931, 1055
BstSLI GKGCMC 4 cut(s) 413, 774, 1253, 1414
BstUI CGCG 3 cut(s) 62, 896, 898
BstV1I GCAGC 4 cut(s) 291, 611, 797, 1299
BstX2I RGATCY 2 cut(s) 537, 1186
BstYI RGATCY 2 cut(s) 537, 1186
Bsu15I ATCGAT 1 cut(s) 1134
Bsu36I CCTNAGG 1 cut(s) 1339
BsuI GTATCC 1 cut(s) 118
BsuRI GGCC 9 cut(s) 65, 359, 445, 594, 888, 930, 936, 1055, 1060
BsuTUI ATCGAT 1 cut(s) 1134
BtgI CCRYGG 3 cut(s) 79, 937, 984
BtgZI GCGATG 2 cut(s) 297, 1320
BtrI CACGTC 1 cut(s) 351
BtsCI GGATG 2 cut(s) 74, 704
BtsIMutI CAGTG 1 cut(s) 952
Cac8I GCNNGC 7 cut(s) 58, 181, 364, 622, 896, 898, 1351
CfoI GCGC 6 cut(s) 62, 896, 898, 900, 1037, 1292
Cfr10I RCCGGY 4 cut(s) 179, 572, 620, 1349
Cfr13I GGNCC 7 cut(s) 383, 425, 443, 929, 1053, 1058, 1108
ClaI ATCGAT 1 cut(s) 1134
CseI GACGC 2 cut(s) 204, 242
Csp6I GTAC 3 cut(s) 376, 721, 967
CspAI ACCGGT 1 cut(s) 572
CviAII CATG 7 cut(s) 363, 447, 609, 760, 938, 1112, 1284
CviQI GTAC 3 cut(s) 376, 721, 967
DdeI CTNAG 5 cut(s) 43, 168, 1220, 1339, 1406
DinI GGCGCC 1 cut(s) 1036
DpnI GATC 5 cut(s) 539, 701, 845, 1137, 1188
DpnII GATC 5 cut(s) 537, 699, 843, 1135, 1186
DrdI GACNNNNNNGTC 1 cut(s) 1100
DseDI GACNNNNNNGTC 1 cut(s) 1100
EaeI YGGCCR 5 cut(s) 63, 357, 592, 886, 934
Eco130I CCWWGG 4 cut(s) 439, 459, 595, 937
Eco47I GGWCC 3 cut(s) 383, 425, 1108
Eco47III AGCGCT 1 cut(s) 1291
Eco57I CTGAAG 2 cut(s) 195, 1219
Eco81I CCTNAGG 1 cut(s) 1339
EcoRII CCWGG 3 cut(s) 784, 888, 931
EcoT14I CCWWGG 4 cut(s) 439, 459, 595, 937
EgeI GGCGCC 1 cut(s) 1036
EheI GGCGCC 1 cut(s) 1036
ErhI CCWWGG 4 cut(s) 439, 459, 595, 937
FaeI CATG 7 cut(s) 366, 450, 612, 763, 941, 1115, 1287
FaqI GGGAC 1 cut(s) 881
FatI CATG 7 cut(s) 362, 446, 608, 759, 937, 1111, 1283
FbaI TGATCA 1 cut(s) 843
Fnu4HI GCNGC 7 cut(s) 63, 268, 280, 360, 625, 811, 1288
FokI GGATG 2 cut(s) 61, 691
Fsp4HI GCNGC 7 cut(s) 63, 268, 280, 360, 625, 811, 1288
GlaI GCGC 6 cut(s) 61, 895, 897, 899, 1036, 1291
GluI GCNGC 7 cut(s) 63, 268, 280, 360, 625, 811, 1288
GsuI CTGGAG 1 cut(s) 730
HaeII RGCGCY 2 cut(s) 1038, 1293
HaeIII GGCC 9 cut(s) 65, 359, 445, 594, 888, 930, 936, 1055, 1060
HapII CCGG 9 cut(s) 180, 216, 276, 573, 621, 864, 1056, 1197, 1350
HgaI GACGC 2 cut(s) 204, 242
HhaI GCGC 6 cut(s) 62, 896, 898, 900, 1037, 1292
Hin1I GRCGYC 2 cut(s) 150, 1035
Hin1II CATG 7 cut(s) 366, 450, 612, 763, 941, 1115, 1287
Hin6I GCGC 6 cut(s) 60, 894, 896, 898, 1035, 1290
HinP1I GCGC 6 cut(s) 60, 894, 896, 898, 1035, 1290
HindIII AAGCTT 1 cut(s) 1217
HinfI GANTC 5 cut(s) 185, 272, 307, 743, 1041
HpaII CCGG 9 cut(s) 180, 216, 276, 573, 621, 864, 1056, 1197, 1350
HphI GGTGA 4 cut(s) 562, 587, 730, 988
Hpy166II GTNNAC 2 cut(s) 83, 772
Hpy188I TCNGA 4 cut(s) 114, 829, 1238, 1380
Hpy188III TCNNGA 4 cut(s) 311, 530, 670, 1197
Hpy8I GTNNAC 2 cut(s) 83, 772
Hpy99I CGWCG 4 cut(s) 114, 638, 887, 1261
HpyAV CCTTC 3 cut(s) 169, 667, 1177
HpyCH4III ACNGT 4 cut(s) 302, 560, 617, 1000
HpyCH4IV ACGT 7 cut(s) 150, 350, 753, 834, 918, 1266, 1370
HpyCH4V TGCA 9 cut(s) 74, 104, 366, 450, 686, 772, 1011, 1287, 1375
HpyF10VI GCNNNNNNNGC 7 cut(s) 62, 71, 164, 276, 630, 639, 1355
HpyF3I CTNAG 5 cut(s) 43, 168, 1220, 1339, 1406
HpySE526I ACGT 7 cut(s) 150, 350, 753, 834, 918, 1266, 1370
Hsp92I GRCGYC 2 cut(s) 150, 1035
Hsp92II CATG 7 cut(s) 366, 450, 612, 763, 941, 1115, 1287
HspAI GCGC 6 cut(s) 60, 894, 896, 898, 1035, 1290
KasI GGCGCC 1 cut(s) 1034
Kpn2I TCCGGA 1 cut(s) 1196
KroI GCCGGC 3 cut(s) 179, 620, 1349
KroNI GCCGGC 3 cut(s) 181, 622, 1351
Ksp22I TGATCA 1 cut(s) 843
Kzo9I GATC 5 cut(s) 537, 699, 843, 1135, 1186
LmnI GCTCC 5 cut(s) 114, 241, 461, 463, 1346
Lsp1109I GCAGC 4 cut(s) 291, 611, 797, 1299
LweI GCATC 6 cut(s) 83, 91, 304, 861, 951, 1384
MaeII ACGT 7 cut(s) 150, 350, 753, 834, 918, 1266, 1370
MaeIII GTNAC 9 cut(s) 151, 568, 754, 904, 919, 994, 1000, 1275, 1384
MalI GATC 5 cut(s) 539, 701, 845, 1137, 1188
MboI GATC 5 cut(s) 537, 699, 843, 1135, 1186
MfeI CAATTG 1 cut(s) 1006
MflI RGATCY 2 cut(s) 537, 1186
MhlI GDGCHC 4 cut(s) 413, 774, 1253, 1414
MlsI TGGCCA 2 cut(s) 594, 936
MluCI AATT 5 cut(s) 480, 665, 1006, 1083, 1316
MluNI TGGCCA 2 cut(s) 594, 936
Mly113I GGCGCC 1 cut(s) 1035
MlyI GAGTC 2 cut(s) 266, 737
MmeI TCCRAC 4 cut(s) 92, 677, 852, 931
Mox20I TGGCCA 2 cut(s) 594, 936
MroI TCCGGA 1 cut(s) 1196
MroNI GCCGGC 3 cut(s) 179, 620, 1349
MscI TGGCCA 2 cut(s) 594, 936
MseI TTAA 3 cut(s) 345, 678, 1245
MslI CAYNNNNRTG 2 cut(s) 1282, 1380
Msp20I TGGCCA 2 cut(s) 594, 936
MspI CCGG 9 cut(s) 180, 216, 276, 573, 621, 864, 1056, 1197, 1350
MspR9I CCNGG 6 cut(s) 216, 786, 865, 890, 933, 1057
MunI CAATTG 1 cut(s) 1006
Mva1269I GAATGC 2 cut(s) 338, 688
MvaI CCWGG 3 cut(s) 786, 890, 933
MvnI CGCG 3 cut(s) 62, 896, 898
MwoI GCNNNNNNNGC 7 cut(s) 62, 71, 164, 276, 630, 639, 1355
NaeI GCCGGC 3 cut(s) 181, 622, 1351
NarI GGCGCC 1 cut(s) 1035
NciI CCSGG 3 cut(s) 216, 865, 1057
NcoI CCATGG 1 cut(s) 937
NdeII GATC 5 cut(s) 537, 699, 843, 1135, 1186
NgoMIV GCCGGC 3 cut(s) 179, 620, 1349
NlaIII CATG 7 cut(s) 366, 450, 612, 763, 941, 1115, 1287
NmuCI GTSAC 5 cut(s) 151, 568, 994, 1000, 1275
NspI RCATGY 1 cut(s) 366
NspV TTCGAA 2 cut(s) 305, 546
PaeI GCATGC 1 cut(s) 366
PauI GCGCGC 2 cut(s) 894, 896
PcsI WCGNNNNNNNCGW 4 cut(s) 128, 981, 996, 1098
PctI GAATGC 2 cut(s) 338, 688
PdiI GCCGGC 3 cut(s) 181, 622, 1351
PfeI GAWTC 3 cut(s) 185, 307, 1041
PflFI GACNNNGTC 1 cut(s) 833
PinAI ACCGGT 1 cut(s) 572
PkrI GCNGC 7 cut(s) 64, 269, 281, 361, 626, 812, 1289
PleI GAGTC 2 cut(s) 266, 737
PluTI GGCGCC 1 cut(s) 1038
PpsI GAGTC 2 cut(s) 266, 737
Psp1406I AACGTT 3 cut(s) 753, 918, 1266
Psp6I CCWGG 3 cut(s) 784, 888, 931
PspGI CCWGG 3 cut(s) 784, 888, 931
PspPI GGNCC 7 cut(s) 383, 425, 443, 929, 1053, 1058, 1108
PsrI GAACNNNNNNTAC 2 cut(s) 704, 736
PsuI RGATCY 2 cut(s) 537, 1186
PsyI GACNNNGTC 1 cut(s) 833
PteI GCGCGC 2 cut(s) 894, 896
RsaI GTAC 3 cut(s) 377, 722, 968
RsaNI GTAC 3 cut(s) 376, 721, 967
RseI CAYNNNNRTG 2 cut(s) 1282, 1380
SaqAI TTAA 3 cut(s) 345, 678, 1245
SatI GCNGC 7 cut(s) 63, 268, 280, 360, 625, 811, 1288
Sau3AI GATC 5 cut(s) 537, 699, 843, 1135, 1186
Sau96I GGNCC 7 cut(s) 383, 425, 443, 929, 1053, 1058, 1108
ScaI AGTACT 1 cut(s) 722
SchI GAGTC 2 cut(s) 266, 737
ScrFI CCNGG 6 cut(s) 216, 786, 865, 890, 933, 1057
SduI GDGCHC 4 cut(s) 413, 774, 1253, 1414
SfaNI GCATC 6 cut(s) 83, 91, 304, 861, 951, 1384
SfoI GGCGCC 1 cut(s) 1036
SfuI TTCGAA 2 cut(s) 305, 546
SgrAI CRCCGGYG 1 cut(s) 572
SinI GGWCC 3 cut(s) 383, 425, 1108
SmiMI CAYNNNNRTG 2 cut(s) 1282, 1380
SphI GCATGC 1 cut(s) 366
Sse9I AATT 5 cut(s) 480, 665, 1006, 1083, 1316
SsiI CCGC 6 cut(s) 9, 62, 219, 267, 360, 1356
SspDI GGCGCC 1 cut(s) 1034
SspI AATATT 1 cut(s) 553
StyD4I CCNGG 6 cut(s) 214, 784, 863, 888, 931, 1055
StyI CCWWGG 4 cut(s) 439, 459, 595, 937
TaaI ACNGT 4 cut(s) 302, 560, 617, 1000
TaiI ACGT 7 cut(s) 153, 353, 756, 837, 921, 1269, 1373
TaqI TCGA 7 cut(s) 305, 546, 693, 1019, 1092, 1134, 1329
TasI AATT 5 cut(s) 480, 665, 1006, 1083, 1316
TatI WGTACW 3 cut(s) 375, 720, 966
TauI GCSGC 3 cut(s) 65, 270, 362
TfiI GAWTC 3 cut(s) 185, 307, 1041
Tru1I TTAA 3 cut(s) 345, 678, 1245
Tru9I TTAA 3 cut(s) 345, 678, 1245
TscAI CASTG 1 cut(s) 952
TseFI GTSAC 5 cut(s) 151, 568, 994, 1000, 1275
TseI GCWGC 4 cut(s) 279, 624, 810, 1287
Tsp45I GTSAC 5 cut(s) 151, 568, 994, 1000, 1275
TspDTI ATGAA 3 cut(s) 331, 716, 776
TspGWI ACGGA 3 cut(s) 137, 871, 1161
TspRI CASTG 1 cut(s) 952
Tth111I GACNNNGTC 1 cut(s) 833
VneI GTGCAC 1 cut(s) 770
VpaK11BI GGWCC 3 cut(s) 383, 425, 1108
XapI RAATTY 2 cut(s) 480, 665
XceI RCATGY 1 cut(s) 366
ZraI GACGTC 1 cut(s) 151
ZrmI AGTACT 1 cut(s) 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.