MD03G1291400.v1.1

Exopolygalacturonase clone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016557
Physical Location & Seq
Forward (+)
36873989 .. 36876173
2185 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1291400.v1.1.491

Sequence Viewer

Length: 1620 bp
ATGGGATTGAGGTCACCAAATATGGGGGGCGTTGCAAGAAGAACATCAGCCATGGCTGTGTTTCTTCTCGGCTTCGCTTTGCTCTCCTGCGTGGCCCAAGCTGCATCCCGTGGACATCTTTTCAGGGCTGGTGCCCGTCGTAGCGACACTACCAAGAGGAAAGTGTTTGATGTCACAGCCTTCGGTGCTAAGACCGAAGACCAGATGAATAGAGGAAGTAAGAAGCCTAATGTTGTTGGCAAGTTGAGTACTGGGGAACTGCCCGGTGGAGGTATACCTGGCCTCCCTGGTGGTACTGTGGAGGAGAGTTATGATGATGGCGAGAAAATTGATGATCCTAATGGATTGGCATTCATCCGGACATGGGTAGCCGCATGCAGAGGAAACTACAAGGGTCCAACTAAGGTTGTGATCCCCAAAGGAACATTCTTGACAGGACCTGTGGTGTTCCAAGGGCCATGCAACAGCTCAACTGAGCCCATCGTCGTTGAAGTTCAAGGATATGTCAAAGCCAGCACCGATCTCAGTCAATACTCCTCTCCCGAGTGGTTTTCATTTGAGTTGGTTGACGGCTTGATCGTCACGGGTGACGGCACTTTTGACGGGCAAGGTACATGGACAACCAGTGGCAGCAAGGACTCCGACAAAGCTGCTCAAGCCCCATCCTCACTTAAATTCAACAAAGTGAACAACAGCCTGATCGAAAGGATCACATCCTTAAATAGCAAATTCTTCCACACACACATGTTCGGCTGCAACAACGTGACAATGAGGAATGTCCACCTCACTGCTCCTGGAGATAGCCCTAACACAGATGGCGTGTACATCAGCGCTTCGGACCACATCAAAGTGCTTAACAGTGTTATCGCAACTGGGGGTGACTGCGTTTCCATCGGGCCAGGCGCTTACGATGTTACCGTTAAAAACATCACGTGTGGTCCAGGGCATGGCATCAGTATCGGGAGCCTCGGAAAGGACCCTGACGAACTGAGCGTCAGTGAGATTCACGTCAGCAATTGCACATTGAGAAACACCACCTATGGTGCCAGAATCAAGACTTCGGCTGGCAAAAGTGCAGGAGAAGTGACCGGAATCGTCTACGAAGACATTATCATGGACCAAGTTCAAAACCCTATTATTATAGATCAGTACTACGGTAACACGAAGGTGGTAAAAAAAATCATCGATGGAAATAAAAAGATGGTAGTCCCAGGAAATAAAAAGAGCGTAGGAGCTGGATTAAAAAAGAAGGTAGGAGCTGGATTAAAAAAGAAGGTAGGAGCTGGATCAAAAAAGAAGGTTGGAGTAGGAAAACAGATGGAACTGCAAGGTGGAAAGAAAAAGAGCGTAAGTAGGAACGATGGAGCTGGGGTACCTTCTAGGCCAGTATCGAAGTGGAAGATCAGTGACGTTCACTTCAGAAACATTAGGGGCACCTCAGCCGGAAAGGTTGCCGTTTCGTTAGCTTGCAGCTCTGTAAACCCATGCGAGGGTGTTAAGTTCACCGACATTAACCTTTCCTACAAGGGCACCAGTAATAAAAAAACGGCGCTTAAATCTGAATGTTTCAACGCCCAGATTACACCAAAGGGCGTACAAAACCCACCACTCTGCCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0016787 GO:0016798 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

540

Amino Acids

56.89

Weight (kDa)

9.56

Isoelectric Point (pI)

26.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 133 - 392 8.7e-70 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 461 - 514 3.8e-09 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016557)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g26010
malus_domestica MD03G1291400.v1.1 MD11G1306900.v1.1
prunus_persica Prupe.8G264500_v2.0.a1
pyrus_communis pycom03g23080 pycom11g26850
rosa_chinensis RchiOBHm_Chr6g0294381
rosa_laevigata RLG00000011876
rosa_multiflora Rmu_sc0005369.1_g000014
rosa_roxburghii Rroxscaffold_7G00173430
rosa_rugosa Rorug06G0243900
rosa_samantha Rh6AG356500 Rh6CG370500
rosa_wichuraiana Rw6G031070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1372
AccB1I GGYRCC 5 cut(s) 131, 1043, 1372, 1433, 1529
AccB7I CCANNNNNTGG 1 cut(s) 947
AccI GTMKAC 2 cut(s) 274, 1098
AccIII TCCGGA 1 cut(s) 357
AciI CCGC 1 cut(s) 372
AclWI GGATC 4 cut(s) 329, 406, 716, 1294
AcsI RAATTY 2 cut(s) 674, 728
AcuI CTGAAG 1 cut(s) 1402
AcvI CACGTG 1 cut(s) 933
AfaI GTAC 7 cut(s) 250, 295, 613, 824, 1151, 1374, 1596
AfeI AGCGCT 1 cut(s) 832
AfiI CCNNNNNNNGG 7 cut(s) 23, 269, 364, 947, 973, 1489, 1490
AflIII ACRYGT 2 cut(s) 744, 932
AgsI TTSAA 5 cut(s) 491, 497, 679, 1127, 1570
AjiI CACGTC 1 cut(s) 1009
AjnI CCWGG 6 cut(s) 277, 286, 793, 898, 940, 1210
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AleI CACNNNNGTG 1 cut(s) 1166
AluBI AGCT 9 cut(s) 101, 468, 650, 1235, 1259, 1283, 1367, 1466, 1473
AluI AGCT 9 cut(s) 101, 468, 650, 1235, 1259, 1283, 1367, 1466, 1473
AlwI GGATC 4 cut(s) 329, 406, 716, 1294
AlwNI CAGNNNCTG 1 cut(s) 440
Ama87I CYCGRG 1 cut(s) 542
Aor13HI TCCGGA 1 cut(s) 357
Aor51HI AGCGCT 1 cut(s) 832
AoxI GGCC 5 cut(s) 93, 280, 455, 896, 1382
ApeKI GCWGC 5 cut(s) 101, 630, 650, 753, 1470
ApoI RAATTY 2 cut(s) 674, 728
ArsI GACNNNNNNTTYG 4 cut(s) 581, 613, 978, 1010
Asp718I GGTACC 1 cut(s) 1372
AspLEI GCGC 3 cut(s) 833, 905, 1552
AspS9I GGNCC 9 cut(s) 94, 395, 437, 455, 838, 896, 938, 976, 1117
AsuC2I CCSGG 1 cut(s) 264
AsuHPI GGTGA 4 cut(s) 6, 599, 890, 1495
AvaI CYCGRG 1 cut(s) 542
AvaII GGWCC 6 cut(s) 395, 437, 838, 938, 976, 1117
BaeGI GKGCMC 3 cut(s) 136, 1436, 1532
BanI GGYRCC 5 cut(s) 131, 1043, 1372, 1433, 1529
BanII GRGCYC 1 cut(s) 480
BbrPI CACGTG 1 cut(s) 933
BbsI GAAGAC 2 cut(s) 204, 1110
BbvCI CCTCAGC 1 cut(s) 1438
BbvI GCAGC 5 cut(s) 88, 637, 642, 740, 1482
BccI CCATC 9 cut(s) 311, 488, 670, 809, 899, 1181, 1195, 1312, 1355
BceAI ACGGC 5 cut(s) 586, 607, 1439, 1563, 1599
BcgI CGANNNNNNTGC 4 cut(s) 632, 666, 940, 974
BciT130I CCWGG 6 cut(s) 279, 288, 795, 900, 942, 1212
BcnI CCSGG 1 cut(s) 264
BfaI CTAG 1 cut(s) 1380
BfoI RGCGCY 3 cut(s) 834, 906, 1553
BisI GCNGC 6 cut(s) 102, 372, 631, 651, 754, 1471
BlsI GCNGC 6 cut(s) 103, 373, 632, 652, 755, 1472
BmcAI AGTACT 2 cut(s) 250, 1151
Bme1390I CCNGG 7 cut(s) 264, 279, 288, 795, 900, 942, 1212
Bme18I GGWCC 6 cut(s) 395, 437, 838, 938, 976, 1117
BmeT110I CYCGRG 1 cut(s) 542
BmgBI CACGTC 1 cut(s) 1009
BmgT120I GGNCC 9 cut(s) 94, 395, 437, 455, 838, 896, 938, 976, 1117
BmiI GGNNCC 8 cut(s) 133, 396, 965, 978, 1045, 1374, 1435, 1531
BmrFI CCNGG 7 cut(s) 264, 279, 288, 795, 900, 942, 1212
BmrI ACTGGG 2 cut(s) 261, 882
BmsI GCATC 2 cut(s) 113, 960
BmuI ACTGGG 2 cut(s) 261, 882
BpiI GAAGAC 2 cut(s) 204, 1110
BpmI CTGGAG 1 cut(s) 816
Bpu10I CCTNAGC 1 cut(s) 1438
BpuEI CTTGAG 1 cut(s) 639
BpuMI CCSGG 1 cut(s) 264
Bsa29I ATCGAT 1 cut(s) 1185
BsaAI YACGTR 1 cut(s) 933
BsaJI CCNNGG 7 cut(s) 51, 109, 286, 451, 941, 967, 1210
BsaWI WCCGGW 2 cut(s) 357, 1088
BsaXI ACNNNNNCTCC 4 cut(s) 267, 297, 1356, 1386
Bsc4I CCNNNNNNNGG 7 cut(s) 23, 269, 364, 947, 973, 1489, 1490
Bse1I ACTGG 5 cut(s) 256, 624, 877, 1385, 1533
BseAI TCCGGA 1 cut(s) 357
BseBI CCWGG 6 cut(s) 279, 288, 795, 900, 942, 1212
BseCI ATCGAT 1 cut(s) 1185
BseDI CCNNGG 7 cut(s) 51, 109, 286, 451, 941, 967, 1210
BseGI GGATG 4 cut(s) 104, 354, 662, 713
BseLI CCNNNNNNNGG 7 cut(s) 23, 269, 364, 947, 973, 1489, 1490
BseMII CTCAG 4 cut(s) 465, 538, 980, 1452
BseNI ACTGG 5 cut(s) 256, 624, 877, 1385, 1533
BseRI GAGGAG 2 cut(s) 317, 526
BseSI GKGCMC 3 cut(s) 136, 1436, 1532
BseXI GCAGC 5 cut(s) 88, 637, 642, 740, 1482
BseYI CCCAGC 1 cut(s) 1367
BsgI GTGCAG 1 cut(s) 1095
BshFI GGCC 5 cut(s) 95, 282, 457, 898, 1384
BshNI GGYRCC 5 cut(s) 131, 1043, 1372, 1433, 1529
BshVI ATCGAT 1 cut(s) 1185
BsiHKCI CYCGRG 1 cut(s) 542
BsiSI CCGG 4 cut(s) 264, 358, 1089, 1443
BslFI GGGAC 1 cut(s) 1193
BslI CCNNNNNNNGG 7 cut(s) 23, 269, 364, 947, 973, 1489, 1490
BsmFI GGGAC 1 cut(s) 1193
BsmI GAATGC 1 cut(s) 350
BsnI GGCC 5 cut(s) 95, 282, 457, 898, 1384
BsoBI CYCGRG 1 cut(s) 542
Bsp1286I GDGCHC 4 cut(s) 136, 480, 1436, 1532
Bsp13I TCCGGA 1 cut(s) 357
Bsp1407I TGTACA 1 cut(s) 822
Bsp143I GATC 9 cut(s) 334, 411, 520, 576, 699, 708, 1144, 1286, 1401
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 1 cut(s) 372
BspANI GGCC 5 cut(s) 95, 282, 457, 898, 1384
BspCNI CTCAG 4 cut(s) 466, 537, 981, 1451
BspDI ATCGAT 1 cut(s) 1185
BspEI TCCGGA 1 cut(s) 357
BspLI GGNNCC 8 cut(s) 133, 396, 965, 978, 1045, 1374, 1435, 1531
BspPI GGATC 4 cut(s) 329, 406, 716, 1294
BspT107I GGYRCC 5 cut(s) 131, 1043, 1372, 1433, 1529
BsrGI TGTACA 1 cut(s) 822
BsrI ACTGG 5 cut(s) 256, 624, 877, 1385, 1533
BssECI CCNNGG 7 cut(s) 51, 109, 286, 451, 941, 967, 1210
BssMI GATC 9 cut(s) 334, 411, 520, 576, 699, 708, 1144, 1286, 1401
BssNAI GTATAC 1 cut(s) 275
BssT1I CCWWGG 2 cut(s) 51, 451
Bst1107I GTATAC 1 cut(s) 275
Bst2UI CCWGG 6 cut(s) 279, 288, 795, 900, 942, 1212
Bst4CI ACNGT 4 cut(s) 298, 860, 919, 1157
BstAUI TGTACA 1 cut(s) 822
BstBAI YACGTR 1 cut(s) 933
BstC8I GCNNGC 4 cut(s) 376, 514, 1066, 1468
BstDEI CTNAG 6 cut(s) 189, 402, 474, 524, 989, 1438
BstDSI CCRYGG 2 cut(s) 51, 109
BstEII GGTNACC 1 cut(s) 12
BstENI CCTNNNNNAGG 1 cut(s) 971
BstF5I GGATG 4 cut(s) 104, 354, 662, 713
BstH2I RGCGCY 3 cut(s) 834, 906, 1553
BstHHI GCGC 3 cut(s) 833, 905, 1552
BstKTI GATC 9 cut(s) 337, 414, 523, 579, 702, 711, 1147, 1289, 1404
BstMBI GATC 9 cut(s) 334, 411, 520, 576, 699, 708, 1144, 1286, 1401
BstMWI GCNNNNNNNGC 3 cut(s) 101, 185, 656
BstNI CCWGG 6 cut(s) 279, 288, 795, 900, 942, 1212
BstNSI RCATGY 2 cut(s) 378, 748
BstPI GGTNACC 1 cut(s) 12
BstSCI CCNGG 7 cut(s) 262, 277, 286, 793, 898, 940, 1210
BstSLI GKGCMC 3 cut(s) 136, 1436, 1532
BstV1I GCAGC 5 cut(s) 88, 637, 642, 740, 1482
BstV2I GAAGAC 2 cut(s) 204, 1110
BstZ17I GTATAC 1 cut(s) 275
Bsu15I ATCGAT 1 cut(s) 1185
BsuRI GGCC 5 cut(s) 95, 282, 457, 898, 1384
BsuTUI ATCGAT 1 cut(s) 1185
BtgI CCRYGG 2 cut(s) 51, 109
BtrI CACGTC 1 cut(s) 1009
BtsCI GGATG 4 cut(s) 104, 354, 662, 713
BtsI GCAGTG 1 cut(s) 786
BtsIMutI CAGTG 5 cut(s) 631, 786, 865, 1003, 1411
Cac8I GCNNGC 4 cut(s) 376, 514, 1066, 1468
CaiI CAGNNNCTG 1 cut(s) 440
CfoI GCGC 3 cut(s) 833, 905, 1552
Cfr13I GGNCC 9 cut(s) 94, 395, 437, 455, 838, 896, 938, 976, 1117
ClaI ATCGAT 1 cut(s) 1185
CseI GACGC 1 cut(s) 982
Csp6I GTAC 7 cut(s) 249, 294, 612, 823, 1150, 1373, 1595
CviAII CATG 9 cut(s) 52, 363, 375, 459, 615, 745, 947, 1114, 1485
CviQI GTAC 7 cut(s) 249, 294, 612, 823, 1150, 1373, 1595
DdeI CTNAG 6 cut(s) 189, 402, 474, 524, 989, 1438
DpnI GATC 9 cut(s) 336, 413, 522, 578, 701, 710, 1146, 1288, 1403
DpnII GATC 9 cut(s) 334, 411, 520, 576, 699, 708, 1144, 1286, 1401
Eco130I CCWWGG 2 cut(s) 51, 451
Eco24I GRGCYC 1 cut(s) 480
Eco47I GGWCC 6 cut(s) 395, 437, 838, 938, 976, 1117
Eco47III AGCGCT 1 cut(s) 832
Eco57I CTGAAG 1 cut(s) 1402
Eco72I CACGTG 1 cut(s) 933
Eco88I CYCGRG 1 cut(s) 542
Eco91I GGTNACC 1 cut(s) 12
EcoNI CCTNNNNNAGG 1 cut(s) 971
EcoO109I RGGNCCY 2 cut(s) 437, 976
EcoO65I GGTNACC 1 cut(s) 12
EcoRII CCWGG 6 cut(s) 277, 286, 793, 898, 940, 1210
EcoT14I CCWWGG 2 cut(s) 51, 451
EcoT38I GRGCYC 1 cut(s) 480
ErhI CCWWGG 2 cut(s) 51, 451
FaeI CATG 9 cut(s) 55, 366, 378, 462, 618, 748, 950, 1117, 1488
FaqI GGGAC 1 cut(s) 1193
FatI CATG 9 cut(s) 51, 362, 374, 458, 614, 744, 946, 1113, 1484
FblI GTMKAC 2 cut(s) 274, 1098
Fnu4HI GCNGC 6 cut(s) 102, 372, 631, 651, 754, 1471
FokI GGATG 4 cut(s) 91, 341, 649, 700
FriOI GRGCYC 1 cut(s) 480
Fsp4HI GCNGC 6 cut(s) 102, 372, 631, 651, 754, 1471
FspBI CTAG 1 cut(s) 1380
GlaI GCGC 3 cut(s) 832, 904, 1551
GluI GCNGC 6 cut(s) 102, 372, 631, 651, 754, 1471
GsaI CCCAGC 1 cut(s) 1371
GsuI CTGGAG 1 cut(s) 816
HaeII RGCGCY 3 cut(s) 834, 906, 1553
HaeIII GGCC 5 cut(s) 95, 282, 457, 898, 1384
HapII CCGG 4 cut(s) 264, 358, 1089, 1443
HgaI GACGC 1 cut(s) 982
HhaI GCGC 3 cut(s) 833, 905, 1552
Hin1II CATG 9 cut(s) 55, 366, 378, 462, 618, 748, 950, 1117, 1488
Hin6I GCGC 3 cut(s) 831, 903, 1550
HinP1I GCGC 3 cut(s) 831, 903, 1550
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HinfI GANTC 4 cut(s) 638, 1003, 1050, 1092
HpaII CCGG 4 cut(s) 264, 358, 1089, 1443
HphI GGTGA 4 cut(s) 6, 599, 890, 1495
Hpy188I TCNGA 5 cut(s) 643, 838, 971, 1421, 1561
Hpy188III TCNNGA 5 cut(s) 358, 430, 542, 961, 1054
Hpy99I CGWCG 2 cut(s) 141, 488
HpyAV CCTTC 6 cut(s) 190, 1159, 1243, 1267, 1291, 1386
HpyCH4III ACNGT 4 cut(s) 298, 860, 919, 1157
HpyCH4IV ACGT 4 cut(s) 762, 932, 1008, 1410
HpyCH4V TGCA 9 cut(s) 35, 104, 378, 462, 756, 1020, 1076, 1327, 1470
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 185, 656
HpyF3I CTNAG 6 cut(s) 189, 402, 474, 524, 989, 1438
HpySE526I ACGT 4 cut(s) 762, 932, 1008, 1410
Hsp92II CATG 9 cut(s) 55, 366, 378, 462, 618, 748, 950, 1117, 1488
HspAI GCGC 3 cut(s) 831, 903, 1550
Kpn2I TCCGGA 1 cut(s) 357
KpnI GGTACC 1 cut(s) 1376
Kzo9I GATC 9 cut(s) 334, 411, 520, 576, 699, 708, 1144, 1286, 1401
LmnI GCTCC 6 cut(s) 796, 963, 1232, 1256, 1280, 1364
Lsp1109I GCAGC 5 cut(s) 88, 637, 642, 740, 1482
LweI GCATC 2 cut(s) 113, 960
MaeI CTAG 1 cut(s) 1380
MaeII ACGT 4 cut(s) 762, 932, 1008, 1410
MalI GATC 9 cut(s) 336, 413, 522, 578, 701, 710, 1146, 1288, 1403
MboI GATC 9 cut(s) 334, 411, 520, 576, 699, 708, 1144, 1286, 1401
MboII GAAGA 6 cut(s) 51, 56, 209, 724, 1115, 1411
MfeI CAATTG 1 cut(s) 1015
MhlI GDGCHC 4 cut(s) 136, 480, 1436, 1532
MluCI AATT 4 cut(s) 327, 674, 728, 1015
MlyI GAGTC 1 cut(s) 632
MmeI TCCRAC 3 cut(s) 422, 666, 1282
MroI TCCGGA 1 cut(s) 357
MslI CAYNNNNRTG 5 cut(s) 56, 743, 848, 1112, 1166
MspI CCGG 4 cut(s) 264, 358, 1089, 1443
MspR9I CCNGG 7 cut(s) 264, 279, 288, 795, 900, 942, 1212
MunI CAATTG 1 cut(s) 1015
Mva1269I GAATGC 1 cut(s) 350
MvaI CCWGG 6 cut(s) 279, 288, 795, 900, 942, 1212
MwoI GCNNNNNNNGC 3 cut(s) 101, 185, 656
NciI CCSGG 1 cut(s) 264
NcoI CCATGG 1 cut(s) 51
NdeII GATC 9 cut(s) 334, 411, 520, 576, 699, 708, 1144, 1286, 1401
NlaIII CATG 9 cut(s) 55, 366, 378, 462, 618, 748, 950, 1117, 1488
NlaIV GGNNCC 8 cut(s) 133, 396, 965, 978, 1045, 1374, 1435, 1531
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 8 cut(s) 12, 172, 580, 587, 763, 878, 1084, 1406
NspI RCATGY 2 cut(s) 378, 748
OliI CACNNNNGTG 1 cut(s) 1166
PaeI GCATGC 1 cut(s) 378
PciI ACATGT 1 cut(s) 744
PcsI WCGNNNNNNNCGW 3 cut(s) 576, 915, 990
PctI GAATGC 1 cut(s) 350
PfeI GAWTC 3 cut(s) 1003, 1050, 1092
PflMI CCANNNNNTGG 1 cut(s) 947
PfoI TCCNGGA 1 cut(s) 793
PkrI GCNGC 6 cut(s) 103, 373, 632, 652, 755, 1472
PleI GAGTC 1 cut(s) 632
PmaCI CACGTG 1 cut(s) 933
PmlI CACGTG 1 cut(s) 933
PpsI GAGTC 1 cut(s) 632
Ppu21I YACGTR 1 cut(s) 933
PpuMI RGGWCCY 2 cut(s) 437, 976
PscI ACATGT 1 cut(s) 744
Psp5II RGGWCCY 2 cut(s) 437, 976
Psp6I CCWGG 6 cut(s) 277, 286, 793, 898, 940, 1210
PspCI CACGTG 1 cut(s) 933
PspEI GGTNACC 1 cut(s) 12
PspFI CCCAGC 1 cut(s) 1367
PspGI CCWGG 6 cut(s) 277, 286, 793, 898, 940, 1210
PspN4I GGNNCC 8 cut(s) 133, 396, 965, 978, 1045, 1374, 1435, 1531
PspPI GGNCC 9 cut(s) 94, 395, 437, 455, 838, 896, 938, 976, 1117
PspPPI RGGWCCY 2 cut(s) 437, 976
PstNI CAGNNNCTG 1 cut(s) 440
RsaI GTAC 7 cut(s) 250, 295, 613, 824, 1151, 1374, 1596
RsaNI GTAC 7 cut(s) 249, 294, 612, 823, 1150, 1373, 1595
RseI CAYNNNNRTG 5 cut(s) 56, 743, 848, 1112, 1166
SatI GCNGC 6 cut(s) 102, 372, 631, 651, 754, 1471
Sau3AI GATC 9 cut(s) 334, 411, 520, 576, 699, 708, 1144, 1286, 1401
Sau96I GGNCC 9 cut(s) 94, 395, 437, 455, 838, 896, 938, 976, 1117
ScaI AGTACT 2 cut(s) 250, 1151
SchI GAGTC 1 cut(s) 632
ScrFI CCNGG 7 cut(s) 264, 279, 288, 795, 900, 942, 1212
SduI GDGCHC 4 cut(s) 136, 480, 1436, 1532
SfaNI GCATC 2 cut(s) 113, 960
SinI GGWCC 6 cut(s) 395, 437, 838, 938, 976, 1117
SmiMI CAYNNNNRTG 5 cut(s) 56, 743, 848, 1112, 1166
SmlI CTYRAG 1 cut(s) 654
SmoI CTYRAG 1 cut(s) 654
SphI GCATGC 1 cut(s) 378
Sse9I AATT 4 cut(s) 327, 674, 728, 1015
SsiI CCGC 1 cut(s) 372
SspMI CTAG 1 cut(s) 1380
StyD4I CCNGG 7 cut(s) 262, 277, 286, 793, 898, 940, 1210
StyI CCWWGG 2 cut(s) 51, 451
TaaI ACNGT 4 cut(s) 298, 860, 919, 1157
TaiI ACGT 4 cut(s) 765, 935, 1011, 1413
TaqI TCGA 3 cut(s) 702, 1185, 1391
TaqII GACCGA 1 cut(s) 209
TasI AATT 4 cut(s) 327, 674, 728, 1015
TatI WGTACW 3 cut(s) 248, 822, 1149
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 3 cut(s) 1003, 1050, 1092
TscAI CASTG 5 cut(s) 631, 793, 865, 1003, 1411
TseFI GTSAC 8 cut(s) 12, 172, 580, 587, 763, 878, 1084, 1406
TseI GCWGC 5 cut(s) 101, 630, 650, 753, 1470
Tsp45I GTSAC 8 cut(s) 12, 172, 580, 587, 763, 878, 1084, 1406
TspDTI ATGAA 3 cut(s) 221, 343, 543
TspRI CASTG 5 cut(s) 631, 793, 865, 1003, 1411
Van91I CCANNNNNTGG 1 cut(s) 947
VpaK11BI GGWCC 6 cut(s) 395, 437, 838, 938, 976, 1117
XagI CCTNNNNNAGG 1 cut(s) 971
XapI RAATTY 2 cut(s) 674, 728
XceI RCATGY 2 cut(s) 378, 748
XcmI CCANNNNNNNNNTGG 1 cut(s) 1392
XmiI GTMKAC 2 cut(s) 274, 1098
XspI CTAG 1 cut(s) 1380
ZrmI AGTACT 2 cut(s) 250, 1151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.