MD11G1306900.v1.1

Exopolygalacturonase clone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0016557
Physical Location & Seq
Reverse (-)
42110360 .. 42112670
2311 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1306900.v1.1.491

Sequence Viewer

Length: 1641 bp
ATGTCTATGGGATTGAGGTTGCCAAATATGCGGGGCATTGCAAGGAGAGCATCAGCCAAGGCTGTGTTTCTTCTCGGCTTCGCTTTGCTTTCTTGCGTGGCCCAATCTACATCCCGTGGACATCTTTATAGGGCTGGTGCCCGCAGGAGCGTTCTCGACGCCACCAATAGGAAAGTGTTTGATGTCACGGCCTTCGGTGCTAAGACCGAAGACCAGATGAAAGGAGGAGGTAACAAGGCTAATGTTGTTGGCAAGTTGAGTGTACAGGAACTGCCCGGTGGAGGTATACCAGGCCTCCCCGGTGGTTCTGCGGAGGAGAGTTTTGCTGATGAGAAGATTGATGAATCTGGACCGGATGAAACCGGAGCGGGTGAAAGCCCTGAAGAGGAAAATGGATCGGCATTCATCCGGACGTGGGTAGCCGCATGCAGAGGAAACTACAGTGGTCCAACTAAGGTTGTGATCCCCAAAGGAACATTCTTGATAGGACCTGTGGTGTTCCAAGGGCCATGCAACAGCTCAACTGAGCCCATCGTCGTTGAAGTTCAAGGATATGTCAAAGCCAGCACTGATCTCAGTCAATACTCCTCTCCCGAGTGGTTTACATTTGAGTTGGTTGACGGCTTGATCATCACCGGTGACGGAACTTTTGACGGGCAAGGCCAGAACGTATGGAAAACCAGAGATAACAAGGAATCCAGCAGCGCTTCTAATGCCCCATCGTCACTTAAATTCAACAAAGTAAACAACACCCTGATCGAAGGGATCACATCCTTAAACAGCAAATTCTTCCACACACACATGTTCGGCTGCAAAAATGTGACAATGAGCAATGTCCACCTCATTGCTCCTGGAGATAGCCCTAACACAGATGGCCTGCATATCAGCACTTCGAGCCAGATCAAAGTGCTTAACAGCGTTATGGCAACTGGCGATGACTGCGTTTCCATCGGCCAAGGCTCTCATGATATCACCGTTAAGAACGTCACCTGTGGTCCAGGACATGGCATCAGCATCGGGAGCCTCGGAAAGTACGCGGACGAACTGAGCGTCAGTCAGATTTACGTGAGCAACTGCACATTCAGAAACACCACCAACGGTGCCAGAATCAAGACTTGGGCTGGCGAAAGTGCAGGAGAAGCGACCGGAATCATCTACGAAGACATTATCATGGACCAAGTTCAAAACCCTATTATCATTGATCAGAACTATGGTGCCAAGAAGAAGGTAAAGAAAAACATCTTTGGAAATAAAAAGATGGTAGTGTCAGGAAATAAAAAGAGGGTAAGGGCAGGATTAGGAAAGAAGGTAGGAGCAGGATTAAAAAAGAAGGTAGGAGCAGGAAAAAAGAAGGCACTGCAAGGTGGGAAGAAAAAGAGCGAAGCAGCTGGACCACCTTCTGGACCAGCATCAAAGTGGAAGATCAGTGACGTTCACTTCAGAAACATTAGGGGCACCTCAGCCAGAAACGTTGCCGTTTCGTTAGCTTGCAGCTCTGCAAAACCATGCGAGGGCGTTGAGTTGACCGATATTAACTTTTCCTACCAGGGCACCAGTACGAAAAGCACGGCGCTTACATCTGAGTGTTTCAACGCCAAGATTACATCAAAGGGCGTACAGAACCCACCACTCTGCCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0016787 GO:0016798 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

547

Amino Acids

57.66

Weight (kDa)

9.36

Isoelectric Point (pI)

31.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 150 - 413 8.9e-76 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 466 - 523 2.9e-09 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016557)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g26010
malus_domestica MD03G1291400.v1.1 MD11G1306900.v1.1
prunus_persica Prupe.8G264500_v2.0.a1
pyrus_communis pycom03g23080 pycom11g26850
rosa_chinensis RchiOBHm_Chr6g0294381
rosa_laevigata RLG00000011876
rosa_multiflora Rmu_sc0005369.1_g000014
rosa_roxburghii Rroxscaffold_7G00173430
rosa_rugosa Rorug06G0243900
rosa_samantha Rh6AG356500 Rh6CG370500
rosa_wichuraiana Rw6G031070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 5 cut(s) 137, 1100, 1214, 1454, 1550
AccB7I CCANNNNNTGG 2 cut(s) 1004, 1400
AccBSI CCGCTC 1 cut(s) 368
AccI GTMKAC 1 cut(s) 286
AccII CGCG 1 cut(s) 1037
AccIII TCCGGA 1 cut(s) 408
AciI CCGC 6 cut(s) 31, 142, 311, 368, 423, 1037
AclI AACGTT 1 cut(s) 1470
AclWI GGATC 3 cut(s) 403, 457, 773
AcoI YGGCCR 1 cut(s) 952
AcsI RAATTY 2 cut(s) 731, 785
AcuI CTGAAG 2 cut(s) 402, 1423
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 4 cut(s) 264, 1034, 1558, 1617
AfeI AGCGCT 1 cut(s) 706
AfiI CCNNNNNNNGG 7 cut(s) 168, 281, 385, 415, 1004, 1400, 1511
AflIII ACRYGT 1 cut(s) 801
AgeI ACCGGT 1 cut(s) 635
AgsI TTSAA 5 cut(s) 542, 548, 736, 1184, 1591
AjiI CACGTC 1 cut(s) 414
AjnI CCWGG 4 cut(s) 289, 850, 997, 1545
AjuI GAANNNNNNNTTGG 4 cut(s) 461, 493, 1099, 1131
AluBI AGCT 4 cut(s) 519, 1388, 1487, 1494
AluI AGCT 4 cut(s) 519, 1388, 1487, 1494
AlwI GGATC 3 cut(s) 403, 457, 773
AlwNI CAGNNNCTG 1 cut(s) 271
Ama87I CYCGRG 1 cut(s) 593
Aor13HI TCCGGA 1 cut(s) 408
Aor51HI AGCGCT 1 cut(s) 706
AoxI GGCC 7 cut(s) 99, 189, 292, 506, 661, 874, 952
ApeKI GCWGC 4 cut(s) 702, 810, 1385, 1491
ApoI RAATTY 2 cut(s) 731, 785
ArsI GACNNNNNNTTYG 4 cut(s) 632, 664, 1035, 1067
AsiGI ACCGGT 1 cut(s) 635
AspLEI GCGC 2 cut(s) 707, 1573
AspS9I GGNCC 9 cut(s) 100, 350, 446, 488, 506, 995, 1174, 1391, 1403
AsuC2I CCSGG 2 cut(s) 276, 300
AsuHPI GGTGA 5 cut(s) 383, 625, 650, 964, 979
AvaI CYCGRG 1 cut(s) 593
AvaII GGWCC 7 cut(s) 350, 446, 488, 995, 1174, 1391, 1403
BaeGI GKGCMC 3 cut(s) 142, 1457, 1553
BanI GGYRCC 5 cut(s) 137, 1100, 1214, 1454, 1550
BanII GRGCYC 1 cut(s) 531
BbsI GAAGAC 2 cut(s) 216, 1167
BbvCI CCTCAGC 1 cut(s) 1459
BbvI GCAGC 4 cut(s) 714, 797, 1397, 1503
BccI CCATC 5 cut(s) 539, 727, 866, 956, 1252
BceAI ACGGC 5 cut(s) 204, 637, 1460, 1584, 1620
BcgI CGANNNNNNTGC 2 cut(s) 997, 1031
BciT130I CCWGG 4 cut(s) 291, 852, 999, 1547
BclI TGATCA 2 cut(s) 627, 1201
BcnI CCSGG 2 cut(s) 276, 300
BfmI CTRYAG 1 cut(s) 439
BfoI RGCGCY 2 cut(s) 708, 1574
BisI GCNGC 5 cut(s) 423, 703, 811, 1386, 1492
BlsI GCNGC 5 cut(s) 424, 704, 812, 1387, 1493
Bme1390I CCNGG 6 cut(s) 276, 291, 300, 852, 999, 1547
Bme18I GGWCC 7 cut(s) 350, 446, 488, 995, 1174, 1391, 1403
BmeT110I CYCGRG 1 cut(s) 593
BmgBI CACGTC 1 cut(s) 414
BmgT120I GGNCC 9 cut(s) 100, 350, 446, 488, 506, 995, 1174, 1391, 1403
BmiI GGNNCC 6 cut(s) 139, 1022, 1102, 1216, 1456, 1552
BmrFI CCNGG 6 cut(s) 276, 291, 300, 852, 999, 1547
BmsI GCATC 4 cut(s) 59, 1017, 1023, 1418
BpiI GAAGAC 2 cut(s) 216, 1167
BpmI CTGGAG 1 cut(s) 873
Bpu10I CCTNAGC 1 cut(s) 1459
BpuMI CCSGG 2 cut(s) 276, 300
BsaAI YACGTR 1 cut(s) 1066
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 7 cut(s) 57, 115, 298, 502, 955, 1024, 1546
BsaWI WCCGGW 5 cut(s) 352, 362, 408, 635, 1145
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 7 cut(s) 168, 281, 385, 415, 1004, 1400, 1511
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 2 cut(s) 934, 1554
Bse3DI GCAATG 3 cut(s) 36, 838, 843
BseAI TCCGGA 1 cut(s) 408
BseBI CCWGG 4 cut(s) 291, 852, 999, 1547
BseDI CCNNGG 7 cut(s) 57, 115, 298, 502, 955, 1024, 1546
BseGI GGATG 4 cut(s) 110, 361, 405, 770
BseLI CCNNNNNNNGG 7 cut(s) 168, 281, 385, 415, 1004, 1400, 1511
BseMI GCAATG 3 cut(s) 36, 838, 843
BseMII CTCAG 5 cut(s) 516, 589, 1037, 1473, 1572
BseNI ACTGG 2 cut(s) 934, 1554
BseRI GAGGAG 3 cut(s) 240, 329, 577
BseSI GKGCMC 3 cut(s) 142, 1457, 1553
BseXI GCAGC 4 cut(s) 714, 797, 1397, 1503
BsgI GTGCAG 2 cut(s) 1060, 1152
Bsh1236I CGCG 1 cut(s) 1037
Bsh1285I CGRYCG 1 cut(s) 1146
BshFI GGCC 7 cut(s) 101, 191, 294, 508, 663, 876, 954
BshNI GGYRCC 5 cut(s) 137, 1100, 1214, 1454, 1550
BshTI ACCGGT 1 cut(s) 635
BsiEI CGRYCG 1 cut(s) 1146
BsiHKCI CYCGRG 1 cut(s) 593
BsiSI CCGG 7 cut(s) 276, 300, 353, 363, 409, 636, 1146
BslI CCNNNNNNNGG 7 cut(s) 168, 281, 385, 415, 1004, 1400, 1511
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 7 cut(s) 101, 191, 294, 508, 663, 876, 954
BsoBI CYCGRG 1 cut(s) 593
Bsp1286I GDGCHC 4 cut(s) 142, 531, 1457, 1553
Bsp13I TCCGGA 1 cut(s) 408
Bsp1407I TGTACA 1 cut(s) 262
Bsp143I GATC 9 cut(s) 395, 462, 571, 627, 756, 765, 900, 1201, 1422
BspACI CCGC 6 cut(s) 31, 142, 311, 368, 423, 1037
BspANI GGCC 7 cut(s) 101, 191, 294, 508, 663, 876, 954
BspCNI CTCAG 5 cut(s) 517, 588, 1038, 1472, 1573
BspEI TCCGGA 1 cut(s) 408
BspFNI CGCG 1 cut(s) 1037
BspHI TCATGA 1 cut(s) 964
BspLI GGNNCC 6 cut(s) 139, 1022, 1102, 1216, 1456, 1552
BspPI GGATC 3 cut(s) 403, 457, 773
BspT107I GGYRCC 5 cut(s) 137, 1100, 1214, 1454, 1550
BsrBI CCGCTC 1 cut(s) 368
BsrDI GCAATG 3 cut(s) 36, 838, 843
BsrFI RCCGGY 1 cut(s) 635
BsrGI TGTACA 1 cut(s) 262
BsrI ACTGG 2 cut(s) 934, 1554
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 7 cut(s) 57, 115, 298, 502, 955, 1024, 1546
BssMI GATC 9 cut(s) 395, 462, 571, 627, 756, 765, 900, 1201, 1422
BssNAI GTATAC 1 cut(s) 287
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 3 cut(s) 57, 502, 955
Bst1107I GTATAC 1 cut(s) 287
Bst2UI CCWGG 4 cut(s) 291, 852, 999, 1547
Bst4CI ACNGT 3 cut(s) 443, 976, 1100
Bst6I CTCTTC 1 cut(s) 378
BstACI GRCGYC 1 cut(s) 159
BstAUI TGTACA 1 cut(s) 262
BstBAI YACGTR 1 cut(s) 1066
BstC8I GCNNGC 6 cut(s) 142, 427, 565, 878, 1123, 1489
BstDEI CTNAG 7 cut(s) 201, 453, 525, 575, 1046, 1459, 1581
BstDSI CCRYGG 1 cut(s) 115
BstF5I GGATG 4 cut(s) 110, 361, 405, 770
BstFNI CGCG 1 cut(s) 1037
BstH2I RGCGCY 2 cut(s) 708, 1574
BstHHI GCGC 2 cut(s) 707, 1573
BstKTI GATC 9 cut(s) 398, 465, 574, 630, 759, 768, 903, 1204, 1425
BstMBI GATC 9 cut(s) 395, 462, 571, 627, 756, 765, 900, 1201, 1422
BstMCI CGRYCG 1 cut(s) 1146
BstMWI GCNNNNNNNGC 8 cut(s) 28, 47, 197, 713, 894, 939, 1020, 1139
BstNI CCWGG 4 cut(s) 291, 852, 999, 1547
BstNSI RCATGY 2 cut(s) 429, 805
BstSCI CCNGG 6 cut(s) 274, 289, 298, 850, 997, 1545
BstSFI CTRYAG 1 cut(s) 439
BstSLI GKGCMC 3 cut(s) 142, 1457, 1553
BstUI CGCG 1 cut(s) 1037
BstV1I GCAGC 4 cut(s) 714, 797, 1397, 1503
BstV2I GAAGAC 2 cut(s) 216, 1167
BstZ17I GTATAC 1 cut(s) 287
BsuRI GGCC 7 cut(s) 101, 191, 294, 508, 663, 876, 954
BtgI CCRYGG 1 cut(s) 115
BtgZI GCGATG 1 cut(s) 948
BtrI CACGTC 1 cut(s) 414
BtsCI GGATG 4 cut(s) 110, 361, 405, 770
BtsI GCAGTG 1 cut(s) 1355
BtsIMutI CAGTG 4 cut(s) 448, 567, 1355, 1432
Cac8I GCNNGC 6 cut(s) 142, 427, 565, 878, 1123, 1489
CaiI CAGNNNCTG 1 cut(s) 271
CciI TCATGA 1 cut(s) 964
CfoI GCGC 2 cut(s) 707, 1573
Cfr10I RCCGGY 1 cut(s) 635
Cfr13I GGNCC 9 cut(s) 100, 350, 446, 488, 506, 995, 1174, 1391, 1403
CseI GACGC 2 cut(s) 167, 1039
Csp6I GTAC 4 cut(s) 263, 1033, 1557, 1616
CspAI ACCGGT 1 cut(s) 635
CviAII CATG 7 cut(s) 426, 510, 802, 965, 1004, 1171, 1506
CviQI GTAC 4 cut(s) 263, 1033, 1557, 1616
DdeI CTNAG 7 cut(s) 201, 453, 525, 575, 1046, 1459, 1581
DpnI GATC 9 cut(s) 397, 464, 573, 629, 758, 767, 902, 1203, 1424
DpnII GATC 9 cut(s) 395, 462, 571, 627, 756, 765, 900, 1201, 1422
EaeI YGGCCR 1 cut(s) 952
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Eco130I CCWWGG 3 cut(s) 57, 502, 955
Eco147I AGGCCT 1 cut(s) 294
Eco24I GRGCYC 1 cut(s) 531
Eco32I GATATC 1 cut(s) 970
Eco47I GGWCC 7 cut(s) 350, 446, 488, 995, 1174, 1391, 1403
Eco47III AGCGCT 1 cut(s) 706
Eco57I CTGAAG 2 cut(s) 402, 1423
Eco88I CYCGRG 1 cut(s) 593
EcoO109I RGGNCCY 1 cut(s) 488
EcoRII CCWGG 4 cut(s) 289, 850, 997, 1545
EcoRV GATATC 1 cut(s) 970
EcoT14I CCWWGG 3 cut(s) 57, 502, 955
EcoT38I GRGCYC 1 cut(s) 531
ErhI CCWWGG 3 cut(s) 57, 502, 955
FaeI CATG 7 cut(s) 429, 513, 805, 968, 1007, 1174, 1509
FatI CATG 7 cut(s) 425, 509, 801, 964, 1003, 1170, 1505
FauI CCCGC 3 cut(s) 24, 149, 361
FbaI TGATCA 2 cut(s) 627, 1201
FblI GTMKAC 1 cut(s) 286
Fnu4HI GCNGC 5 cut(s) 423, 703, 811, 1386, 1492
FokI GGATG 4 cut(s) 97, 368, 392, 757
FriOI GRGCYC 1 cut(s) 531
Fsp4HI GCNGC 5 cut(s) 423, 703, 811, 1386, 1492
GlaI GCGC 2 cut(s) 706, 1572
GluI GCNGC 5 cut(s) 423, 703, 811, 1386, 1492
GsuI CTGGAG 1 cut(s) 873
HaeII RGCGCY 2 cut(s) 708, 1574
HaeIII GGCC 7 cut(s) 101, 191, 294, 508, 663, 876, 954
HapII CCGG 7 cut(s) 276, 300, 353, 363, 409, 636, 1146
HgaI GACGC 2 cut(s) 167, 1039
HhaI GCGC 2 cut(s) 707, 1573
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 7 cut(s) 429, 513, 805, 968, 1007, 1174, 1509
Hin6I GCGC 2 cut(s) 705, 1571
HinP1I GCGC 2 cut(s) 705, 1571
HincII GTYRAC 2 cut(s) 619, 1524
HindII GTYRAC 2 cut(s) 619, 1524
HinfI GANTC 4 cut(s) 344, 695, 1107, 1149
HpaII CCGG 7 cut(s) 276, 300, 353, 363, 409, 636, 1146
HphI GGTGA 5 cut(s) 383, 625, 650, 964, 979
Hpy166II GTNNAC 9 cut(s) 119, 263, 287, 603, 619, 745, 838, 1435, 1524
Hpy188I TCNGA 6 cut(s) 1028, 1059, 1085, 1206, 1442, 1582
Hpy8I GTNNAC 9 cut(s) 119, 263, 287, 603, 619, 745, 838, 1435, 1524
Hpy99I CGWCG 2 cut(s) 161, 539
HpyAV CCTTC 7 cut(s) 202, 755, 1219, 1300, 1324, 1345, 1407
HpyCH4III ACNGT 3 cut(s) 443, 976, 1100
HpyCH4IV ACGT 6 cut(s) 413, 669, 984, 1065, 1431, 1470
HpyF10VI GCNNNNNNNGC 8 cut(s) 28, 47, 197, 713, 894, 939, 1020, 1139
HpyF3I CTNAG 7 cut(s) 201, 453, 525, 575, 1046, 1459, 1581
HpySE526I ACGT 6 cut(s) 413, 669, 984, 1065, 1431, 1470
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 7 cut(s) 429, 513, 805, 968, 1007, 1174, 1509
HspAI GCGC 2 cut(s) 705, 1571
Kpn2I TCCGGA 1 cut(s) 408
Ksp22I TGATCA 2 cut(s) 627, 1201
Kzo9I GATC 9 cut(s) 395, 462, 571, 627, 756, 765, 900, 1201, 1422
LmnI GCTCC 6 cut(s) 147, 365, 853, 1020, 1313, 1337
Lsp1109I GCAGC 4 cut(s) 714, 797, 1397, 1503
LweI GCATC 4 cut(s) 59, 1017, 1023, 1418
MaeII ACGT 6 cut(s) 413, 669, 984, 1065, 1431, 1470
MaeIII GTNAC 7 cut(s) 184, 230, 638, 723, 820, 985, 1427
MalI GATC 9 cut(s) 397, 464, 573, 629, 758, 767, 902, 1203, 1424
MbiI CCGCTC 1 cut(s) 368
MboI GATC 9 cut(s) 395, 462, 571, 627, 756, 765, 900, 1201, 1422
MboII GAAGA 9 cut(s) 62, 221, 346, 395, 781, 1172, 1234, 1381, 1432
MhlI GDGCHC 4 cut(s) 142, 531, 1457, 1553
MluCI AATT 2 cut(s) 731, 785
MmeI TCCRAC 1 cut(s) 473
MroI TCCGGA 1 cut(s) 408
MseI TTAA 7 cut(s) 729, 776, 912, 978, 1322, 1533, 1639
MslI CAYNNNNRTG 4 cut(s) 800, 1169, 1414, 1582
MspA1I CMGCKG 1 cut(s) 1388
MspI CCGG 7 cut(s) 276, 300, 353, 363, 409, 636, 1146
MspR9I CCNGG 6 cut(s) 276, 291, 300, 852, 999, 1547
Mva1269I GAATGC 1 cut(s) 401
MvaI CCWGG 4 cut(s) 291, 852, 999, 1547
MvnI CGCG 1 cut(s) 1037
MwoI GCNNNNNNNGC 8 cut(s) 28, 47, 197, 713, 894, 939, 1020, 1139
NciI CCSGG 2 cut(s) 276, 300
NdeII GATC 9 cut(s) 395, 462, 571, 627, 756, 765, 900, 1201, 1422
NlaIII CATG 7 cut(s) 429, 513, 805, 968, 1007, 1174, 1509
NlaIV GGNNCC 6 cut(s) 139, 1022, 1102, 1216, 1456, 1552
NmeAIII GCCGAG 1 cut(s) 54
NmuCI GTSAC 6 cut(s) 184, 638, 723, 820, 985, 1427
NspI RCATGY 2 cut(s) 429, 805
PaeI GCATGC 1 cut(s) 429
PagI TCATGA 1 cut(s) 964
PceI AGGCCT 1 cut(s) 294
PciI ACATGT 1 cut(s) 801
PcsI WCGNNNNNNNCGW 1 cut(s) 1047
PctI GAATGC 1 cut(s) 401
PfeI GAWTC 4 cut(s) 344, 695, 1107, 1149
PflMI CCANNNNNTGG 2 cut(s) 1004, 1400
PfoI TCCNGGA 2 cut(s) 850, 997
PinAI ACCGGT 1 cut(s) 635
PkrI GCNGC 5 cut(s) 424, 704, 812, 1387, 1493
Ppu21I YACGTR 1 cut(s) 1066
PpuMI RGGWCCY 1 cut(s) 488
PscI ACATGT 1 cut(s) 801
Psp1406I AACGTT 1 cut(s) 1470
Psp5II RGGWCCY 1 cut(s) 488
Psp6I CCWGG 4 cut(s) 289, 850, 997, 1545
PspGI CCWGG 4 cut(s) 289, 850, 997, 1545
PspN4I GGNNCC 6 cut(s) 139, 1022, 1102, 1216, 1456, 1552
PspPI GGNCC 9 cut(s) 100, 350, 446, 488, 506, 995, 1174, 1391, 1403
PspPPI RGGWCCY 1 cut(s) 488
PstNI CAGNNNCTG 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 1388
RsaI GTAC 4 cut(s) 264, 1034, 1558, 1617
RsaNI GTAC 4 cut(s) 263, 1033, 1557, 1616
RseI CAYNNNNRTG 4 cut(s) 800, 1169, 1414, 1582
SaqAI TTAA 7 cut(s) 729, 776, 912, 978, 1322, 1533, 1639
SatI GCNGC 5 cut(s) 423, 703, 811, 1386, 1492
Sau3AI GATC 9 cut(s) 395, 462, 571, 627, 756, 765, 900, 1201, 1422
Sau96I GGNCC 9 cut(s) 100, 350, 446, 488, 506, 995, 1174, 1391, 1403
ScrFI CCNGG 6 cut(s) 276, 291, 300, 852, 999, 1547
SduI GDGCHC 4 cut(s) 142, 531, 1457, 1553
SfaNI GCATC 4 cut(s) 59, 1017, 1023, 1418
SfcI CTRYAG 1 cut(s) 439
SgrAI CRCCGGYG 1 cut(s) 635
SinI GGWCC 7 cut(s) 350, 446, 488, 995, 1174, 1391, 1403
SmiMI CAYNNNNRTG 4 cut(s) 800, 1169, 1414, 1582
SphI GCATGC 1 cut(s) 429
Sse9I AATT 2 cut(s) 731, 785
SseBI AGGCCT 1 cut(s) 294
SsiI CCGC 6 cut(s) 31, 142, 311, 368, 423, 1037
StuI AGGCCT 1 cut(s) 294
StyD4I CCNGG 6 cut(s) 274, 289, 298, 850, 997, 1545
StyI CCWWGG 3 cut(s) 57, 502, 955
TaaI ACNGT 3 cut(s) 443, 976, 1100
TaiI ACGT 6 cut(s) 416, 672, 987, 1068, 1434, 1473
TaqI TCGA 3 cut(s) 156, 759, 893
TaqII GACCGA 2 cut(s) 221, 1541
TasI AATT 2 cut(s) 731, 785
TatI WGTACW 1 cut(s) 262
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 4 cut(s) 344, 695, 1107, 1149
Tru1I TTAA 7 cut(s) 729, 776, 912, 978, 1322, 1533, 1639
Tru9I TTAA 7 cut(s) 729, 776, 912, 978, 1322, 1533, 1639
TscAI CASTG 4 cut(s) 448, 574, 1362, 1432
TseFI GTSAC 6 cut(s) 184, 638, 723, 820, 985, 1427
TseI GCWGC 4 cut(s) 702, 810, 1385, 1491
Tsp45I GTSAC 6 cut(s) 184, 638, 723, 820, 985, 1427
TspDTI ATGAA 4 cut(s) 233, 357, 372, 394
TspGWI ACGGA 1 cut(s) 657
TspRI CASTG 4 cut(s) 448, 574, 1362, 1432
Van91I CCANNNNNTGG 2 cut(s) 1004, 1400
VpaK11BI GGWCC 7 cut(s) 350, 446, 488, 995, 1174, 1391, 1403
XapI RAATTY 2 cut(s) 731, 785
XceI RCATGY 2 cut(s) 429, 805
XcmI CCANNNNNNNNNTGG 1 cut(s) 1413
XmiI GTMKAC 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.