pycom03g23080

Exopolygalacturonase clone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0016557
Physical Location & Seq
Forward (+)
23037624 .. 23039700
2077 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g23080.4

Sequence Viewer

Length: 1698 bp
ATGTCTATGGGATTGCGGTCACCAAATATGGGGGGCGTTGCAAGGAGAACATCAGCCATGGCTGTGTTTCTTCTCGGCTTCGCTTTGCTCTCTTGCGTGGCCCAAGCTGCATCCCGTGGACATCTTTTCAGGGCTGGTAACCGTCGTAGCGACACTACCAAGAGGAAAGTGTTTGATGTCACAGCCTTCGGTGCTAAGACCGAAGACCAGATGAATAGAGGAAGTAAGAAGCCTAATGTTGTCGGCAAGTTGAGTACCGGGGAATTGCCCGGTGGAGGTGTACCCGGGGAACTGCCCGGTGGAGGTATACCTGGCCTCCCTGGTGGTACTGAGGAGGAGAGTTATGATGGCAATAATATTGACGATCCTAATGGATTGGTGGGATTACAGAAATCGTTAACACAATTTCATTTCAATGCATTCATCCGGACATGGGTAGCCGCATGCAGAGGAAACTACAAGGGTCCAACTAAGGTTGTGATCCCCAAGGGAACATTCTTGACAGGACCTGTGGTGTTCCAAGGGCCATGCAACAGCTCAACTGAGCCCATCGTCGTTGAAGTTCAAGGATATGTCAAAGCCAGCACCGATATCAGTCAATACTCCTCTCCCGAGTGGTTTTCATTTGAGTTGGTTGACGGCTTGATCGTCACGGGTGACGGAACTTTTGACGGGCAAGGTACATGGACAACCAGTGGCAGCAAGGACTCCGACAAAGCTGCTCAAGCCCCATCCGTAAGCTCTCTAGCTGTTGCACGAATGCAGTCACTTAAATTCAACAAAGTGAACAACACCCTGATCGAAAGGATCACGTCCTTAAATAGCAAATTCTTCCACACACACATGTTCGGCTGCAACAACGTGACAATGAGGAATGTCCACCTCACTGCTCCTGGAGATAGCCCTAACACAGATGGCGTGTACATCAGCGCTTCGGACCACATCAAAGTGCTTAACAGCGTTATCACAACCGGGGGTGACTGCGTTTCCATCGGGCAAGGCGCTCACGATGTCACCGTTAAAAACATCACTTGTGGTCGAGGATATGGCATCAGTATCGGGAGCCTCGGAAAGTACCCTGACGAACTGAGCGTCAGTGAGATTCATGTCAGCAATTGCACATTGAGAAACACCACCTATGGTGCCAGAATCAAGACTTCGGCTGGCAAAAGTGCAGGAGAAGTGACCGGAATCGTCTACGAAGACATTATCATGGACCAAGTTCAAAACCCTATTATTATAGATCAGTACTACGGTAACAAGAAGGTGGTAAAAAAAATCAACGATGGAAATAAAAAGATGGTAGTCCCAGGAAATAAAAAGAACGTAGGAGCTGGATTAAAAAAGAAGGTAGGAGCTGGATTAAAAAAGAAGGTAGGAGCTGGATCAAAAAAGAAGATCGGAGCAGGAAAACAGATGGTACGCATGGATGCACACGATGGAGCTGGAGTACCTTCTAGGCCAGTATCAAAGTGGAAGATCAGTGACGTTCACTTCAGAAACATTAGGGGCACCTCAGCCGGAAAGGTTGCCGTTTCGTTAGCTTGCAGCTCTGTAAACCCATGCGAGGGTGTTAAGTTCACCGACATTAACCTTTCCTACAAGGGCACCAGTAAGAAAAAAACGGCGCTTAAATCTAAGTGTTTCAACGCCCAGATTACACCAAAGGGCGTACAGAACCCACCACTCTGCCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0016787 GO:0016798 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

566

Amino Acids

59.88

Weight (kDa)

9.67

Isoelectric Point (pI)

27.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016557)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g26010
malus_domestica MD03G1291400.v1.1 MD11G1306900.v1.1
prunus_persica Prupe.8G264500_v2.0.a1
pyrus_communis pycom03g23080 pycom11g26850
rosa_chinensis RchiOBHm_Chr6g0294381
rosa_laevigata RLG00000011876
rosa_multiflora Rmu_sc0005369.1_g000014
rosa_roxburghii Rroxscaffold_7G00173430
rosa_rugosa Rorug06G0243900
rosa_samantha Rh6AG356500 Rh6CG370500
rosa_wichuraiana Rw6G031070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 1142, 1511, 1607
AccI GTMKAC 2 cut(s) 307, 1197
AccIII TCCGGA 1 cut(s) 426
AciI CCGC 2 cut(s) 16, 441
AclWI GGATC 4 cut(s) 359, 475, 815, 1393
AcsI RAATTY 2 cut(s) 773, 827
AcuI CTGAAG 1 cut(s) 1480
AfeI AGCGCT 1 cut(s) 931
AfiI CCNNNNNNNGG 6 cut(s) 29, 275, 302, 433, 1567, 1568
AflIII ACRYGT 1 cut(s) 843
AgsI TTSAA 6 cut(s) 415, 560, 566, 778, 1226, 1648
AjiI CACGTC 1 cut(s) 813
AjnI CCWGG 4 cut(s) 310, 319, 892, 1309
AjuI GAANNNNNNNTTGG 2 cut(s) 479, 511
AlwI GGATC 4 cut(s) 359, 475, 815, 1393
AlwNI CAGNNNCTG 1 cut(s) 509
Ama87I CYCGRG 2 cut(s) 284, 611
Aor13HI TCCGGA 1 cut(s) 426
Aor51HI AGCGCT 1 cut(s) 931
AoxI GGCC 4 cut(s) 99, 313, 524, 1460
ApeKI GCWGC 5 cut(s) 107, 699, 719, 852, 1548
ApoI RAATTY 2 cut(s) 773, 827
ArsI GACNNNNNNTTYG 4 cut(s) 650, 682, 1077, 1109
AspLEI GCGC 3 cut(s) 932, 1004, 1630
AspS9I GGNCC 6 cut(s) 100, 464, 506, 524, 937, 1216
AsuC2I CCSGG 6 cut(s) 259, 270, 285, 286, 297, 973
AsuHPI GGTGA 5 cut(s) 12, 668, 989, 1006, 1573
AvaI CYCGRG 2 cut(s) 284, 611
AvaII GGWCC 4 cut(s) 464, 506, 937, 1216
BaeGI GKGCMC 2 cut(s) 1514, 1610
BanI GGYRCC 3 cut(s) 1142, 1511, 1607
BanII GRGCYC 1 cut(s) 549
BbsI GAAGAC 2 cut(s) 210, 1209
BbvCI CCTCAGC 1 cut(s) 1516
BbvI GCAGC 5 cut(s) 94, 706, 711, 839, 1560
BccI CCATC 9 cut(s) 341, 557, 739, 908, 998, 1280, 1294, 1411, 1433
BceAI ACGGC 4 cut(s) 655, 1517, 1641, 1677
BcgI CGANNNNNNTGC 4 cut(s) 701, 735, 1039, 1073
BciT130I CCWGG 4 cut(s) 312, 321, 894, 1311
BcnI CCSGG 6 cut(s) 259, 270, 285, 286, 297, 973
BfaI CTAG 2 cut(s) 746, 1458
BfoI RGCGCY 3 cut(s) 933, 1005, 1631
BisI GCNGC 6 cut(s) 108, 441, 700, 720, 853, 1549
BlsI GCNGC 6 cut(s) 109, 442, 701, 721, 854, 1550
BmcAI AGTACT 1 cut(s) 1250
Bme18I GGWCC 4 cut(s) 464, 506, 937, 1216
BmeT110I CYCGRG 2 cut(s) 284, 611
BmgBI CACGTC 1 cut(s) 813
BmgT120I GGNCC 6 cut(s) 100, 464, 506, 524, 937, 1216
BmiI GGNNCC 5 cut(s) 465, 1064, 1144, 1513, 1609
BmsI GCATC 3 cut(s) 119, 1059, 1420
BpiI GAAGAC 2 cut(s) 210, 1209
BpmI CTGGAG 2 cut(s) 915, 1467
Bpu10I CCTNAGC 1 cut(s) 1516
BpuEI CTTGAG 1 cut(s) 708
BpuMI CCSGG 6 cut(s) 259, 270, 285, 286, 297, 973
BsaWI WCCGGW 2 cut(s) 426, 1187
BsaXI ACNNNNNCTCC 4 cut(s) 300, 330, 1434, 1464
Bsc4I CCNNNNNNNGG 6 cut(s) 29, 275, 302, 433, 1567, 1568
Bse1I ACTGG 3 cut(s) 693, 1463, 1611
BseAI TCCGGA 1 cut(s) 426
BseBI CCWGG 4 cut(s) 312, 321, 894, 1311
BseGI GGATG 4 cut(s) 110, 423, 731, 1435
BseLI CCNNNNNNNGG 6 cut(s) 29, 275, 302, 433, 1567, 1568
BseMII CTCAG 4 cut(s) 321, 534, 1079, 1530
BseNI ACTGG 3 cut(s) 693, 1463, 1611
BseRI GAGGAG 3 cut(s) 347, 350, 595
BseSI GKGCMC 2 cut(s) 1514, 1610
BseXI GCAGC 5 cut(s) 94, 706, 711, 839, 1560
BsgI GTGCAG 1 cut(s) 1194
BshFI GGCC 4 cut(s) 101, 315, 526, 1462
BshNI GGYRCC 3 cut(s) 1142, 1511, 1607
BsiHKCI CYCGRG 2 cut(s) 284, 611
BsiSI CCGG 8 cut(s) 258, 270, 285, 297, 427, 972, 1188, 1521
BslFI GGGAC 1 cut(s) 1292
BslI CCNNNNNNNGG 6 cut(s) 29, 275, 302, 433, 1567, 1568
BsmFI GGGAC 1 cut(s) 1292
BsmI GAATGC 2 cut(s) 419, 765
BsnI GGCC 4 cut(s) 101, 315, 526, 1462
BsoBI CYCGRG 2 cut(s) 284, 611
Bsp1286I GDGCHC 3 cut(s) 549, 1514, 1610
Bsp13I TCCGGA 1 cut(s) 426
Bsp1407I TGTACA 1 cut(s) 921
Bsp143I GATC 9 cut(s) 364, 480, 645, 798, 807, 1243, 1385, 1398, 1479
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 2 cut(s) 16, 441
BspANI GGCC 4 cut(s) 101, 315, 526, 1462
BspCNI CTCAG 4 cut(s) 322, 535, 1080, 1529
BspEI TCCGGA 1 cut(s) 426
BspLI GGNNCC 5 cut(s) 465, 1064, 1144, 1513, 1609
BspPI GGATC 4 cut(s) 359, 475, 815, 1393
BspT107I GGYRCC 3 cut(s) 1142, 1511, 1607
BsrGI TGTACA 1 cut(s) 921
BsrI ACTGG 3 cut(s) 693, 1463, 1611
BssMI GATC 9 cut(s) 364, 480, 645, 798, 807, 1243, 1385, 1398, 1479
BssNAI GTATAC 1 cut(s) 308
BssT1I CCWWGG 3 cut(s) 57, 486, 520
Bst1107I GTATAC 1 cut(s) 308
Bst2UI CCWGG 4 cut(s) 312, 321, 894, 1311
Bst4CI ACNGT 3 cut(s) 143, 1018, 1256
BstAUI TGTACA 1 cut(s) 921
BstC8I GCNNGC 4 cut(s) 445, 583, 1165, 1546
BstDEI CTNAG 7 cut(s) 195, 330, 471, 543, 1088, 1516, 1638
BstDSI CCRYGG 2 cut(s) 57, 115
BstEII GGTNACC 2 cut(s) 18, 137
BstF5I GGATG 4 cut(s) 110, 423, 731, 1435
BstH2I RGCGCY 3 cut(s) 933, 1005, 1631
BstHHI GCGC 3 cut(s) 932, 1004, 1630
BstKTI GATC 9 cut(s) 367, 483, 648, 801, 810, 1246, 1388, 1401, 1482
BstMBI GATC 9 cut(s) 364, 480, 645, 798, 807, 1243, 1385, 1398, 1479
BstMWI GCNNNNNNNGC 3 cut(s) 107, 191, 725
BstNI CCWGG 4 cut(s) 312, 321, 894, 1311
BstNSI RCATGY 2 cut(s) 447, 847
BstPI GGTNACC 2 cut(s) 18, 137
BstSLI GKGCMC 2 cut(s) 1514, 1610
BstV1I GCAGC 5 cut(s) 94, 706, 711, 839, 1560
BstV2I GAAGAC 2 cut(s) 210, 1209
BstZ17I GTATAC 1 cut(s) 308
BsuRI GGCC 4 cut(s) 101, 315, 526, 1462
BtgI CCRYGG 2 cut(s) 57, 115
BtrI CACGTC 1 cut(s) 813
BtsCI GGATG 4 cut(s) 110, 423, 731, 1435
BtsI GCAGTG 1 cut(s) 885
BtsIMutI CAGTG 4 cut(s) 700, 885, 1102, 1489
Cac8I GCNNGC 4 cut(s) 445, 583, 1165, 1546
CaiI CAGNNNCTG 1 cut(s) 509
CfoI GCGC 3 cut(s) 932, 1004, 1630
Cfr13I GGNCC 6 cut(s) 100, 464, 506, 524, 937, 1216
Cfr9I CCCGGG 1 cut(s) 284
CseI GACGC 1 cut(s) 1081
DdeI CTNAG 7 cut(s) 195, 330, 471, 543, 1088, 1516, 1638
DpnI GATC 9 cut(s) 366, 482, 647, 800, 809, 1245, 1387, 1400, 1481
DpnII GATC 9 cut(s) 364, 480, 645, 798, 807, 1243, 1385, 1398, 1479
Eco130I CCWWGG 3 cut(s) 57, 486, 520
Eco24I GRGCYC 1 cut(s) 549
Eco32I GATATC 1 cut(s) 592
Eco47I GGWCC 4 cut(s) 464, 506, 937, 1216
Eco47III AGCGCT 1 cut(s) 931
Eco57I CTGAAG 1 cut(s) 1480
Eco88I CYCGRG 2 cut(s) 284, 611
Eco91I GGTNACC 2 cut(s) 18, 137
EcoO109I RGGNCCY 1 cut(s) 506
EcoO65I GGTNACC 2 cut(s) 18, 137
EcoRII CCWGG 4 cut(s) 310, 319, 892, 1309
EcoRV GATATC 1 cut(s) 592
EcoT14I CCWWGG 3 cut(s) 57, 486, 520
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 1 cut(s) 549
ErhI CCWWGG 3 cut(s) 57, 486, 520
FaqI GGGAC 1 cut(s) 1292
FblI GTMKAC 2 cut(s) 307, 1197
Fnu4HI GCNGC 6 cut(s) 108, 441, 700, 720, 853, 1549
FokI GGATG 4 cut(s) 97, 410, 718, 1442
FriOI GRGCYC 1 cut(s) 549
Fsp4HI GCNGC 6 cut(s) 108, 441, 700, 720, 853, 1549
FspBI CTAG 2 cut(s) 746, 1458
GlaI GCGC 3 cut(s) 931, 1003, 1629
GluI GCNGC 6 cut(s) 108, 441, 700, 720, 853, 1549
GsuI CTGGAG 2 cut(s) 915, 1467
HaeII RGCGCY 3 cut(s) 933, 1005, 1631
HaeIII GGCC 4 cut(s) 101, 315, 526, 1462
HapII CCGG 8 cut(s) 258, 270, 285, 297, 427, 972, 1188, 1521
HgaI GACGC 1 cut(s) 1081
HhaI GCGC 3 cut(s) 932, 1004, 1630
Hin6I GCGC 3 cut(s) 930, 1002, 1628
HinP1I GCGC 3 cut(s) 930, 1002, 1628
HincII GTYRAC 2 cut(s) 399, 637
HindII GTYRAC 2 cut(s) 399, 637
HinfI GANTC 4 cut(s) 707, 1102, 1149, 1191
HpaI GTTAAC 1 cut(s) 399
HpaII CCGG 8 cut(s) 258, 270, 285, 297, 427, 972, 1188, 1521
HphI GGTGA 5 cut(s) 12, 668, 989, 1006, 1573
Hpy188I TCNGA 5 cut(s) 712, 937, 1070, 1403, 1499
Hpy188III TCNNGA 6 cut(s) 427, 499, 611, 1007, 1060, 1153
Hpy99I CGWCG 2 cut(s) 147, 557
HpyAV CCTTC 5 cut(s) 196, 1258, 1342, 1366, 1464
HpyCH4III ACNGT 3 cut(s) 143, 1018, 1256
HpyCH4IV ACGT 4 cut(s) 812, 861, 1326, 1488
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 191, 725
HpyF3I CTNAG 7 cut(s) 195, 330, 471, 543, 1088, 1516, 1638
HpySE526I ACGT 4 cut(s) 812, 861, 1326, 1488
HspAI GCGC 3 cut(s) 930, 1002, 1628
Kpn2I TCCGGA 1 cut(s) 426
KspAI GTTAAC 1 cut(s) 399
Kzo9I GATC 9 cut(s) 364, 480, 645, 798, 807, 1243, 1385, 1398, 1479
LmnI GCTCC 7 cut(s) 895, 1062, 1331, 1355, 1379, 1403, 1442
Lsp1109I GCAGC 5 cut(s) 94, 706, 711, 839, 1560
LweI GCATC 3 cut(s) 119, 1059, 1420
MaeI CTAG 2 cut(s) 746, 1458
MaeII ACGT 4 cut(s) 812, 861, 1326, 1488
MalI GATC 9 cut(s) 366, 482, 647, 800, 809, 1245, 1387, 1400, 1481
MboI GATC 9 cut(s) 364, 480, 645, 798, 807, 1243, 1385, 1398, 1479
MboII GAAGA 6 cut(s) 62, 215, 823, 1214, 1408, 1489
MfeI CAATTG 1 cut(s) 1114
MhlI GDGCHC 3 cut(s) 549, 1514, 1610
MluCI AATT 5 cut(s) 263, 404, 773, 827, 1114
MlyI GAGTC 1 cut(s) 701
MmeI TCCRAC 2 cut(s) 491, 735
Mph1103I ATGCAT 1 cut(s) 421
MroI TCCGGA 1 cut(s) 426
MslI CAYNNNNRTG 5 cut(s) 62, 414, 842, 947, 1211
MspI CCGG 8 cut(s) 258, 270, 285, 297, 427, 972, 1188, 1521
MunI CAATTG 1 cut(s) 1114
Mva1269I GAATGC 2 cut(s) 419, 765
MvaI CCWGG 4 cut(s) 312, 321, 894, 1311
MwoI GCNNNNNNNGC 3 cut(s) 107, 191, 725
NciI CCSGG 6 cut(s) 259, 270, 285, 286, 297, 973
NcoI CCATGG 1 cut(s) 57
NdeII GATC 9 cut(s) 364, 480, 645, 798, 807, 1243, 1385, 1398, 1479
NlaIV GGNNCC 5 cut(s) 465, 1064, 1144, 1513, 1609
NmeAIII GCCGAG 1 cut(s) 54
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 2 cut(s) 447, 847
PaeI GCATGC 1 cut(s) 447
PciI ACATGT 1 cut(s) 843
PcsI WCGNNNNNNNCGW 3 cut(s) 645, 1014, 1089
PctI GAATGC 2 cut(s) 419, 765
PfeI GAWTC 3 cut(s) 1102, 1149, 1191
PfoI TCCNGGA 1 cut(s) 892
PkrI GCNGC 6 cut(s) 109, 442, 701, 721, 854, 1550
PleI GAGTC 1 cut(s) 701
PpsI GAGTC 1 cut(s) 701
PpuMI RGGWCCY 1 cut(s) 506
PscI ACATGT 1 cut(s) 843
Psp5II RGGWCCY 1 cut(s) 506
Psp6I CCWGG 4 cut(s) 310, 319, 892, 1309
PspEI GGTNACC 2 cut(s) 18, 137
PspGI CCWGG 4 cut(s) 310, 319, 892, 1309
PspN4I GGNNCC 5 cut(s) 465, 1064, 1144, 1513, 1609
PspPI GGNCC 6 cut(s) 100, 464, 506, 524, 937, 1216
PspPPI RGGWCCY 1 cut(s) 506
PstNI CAGNNNCTG 1 cut(s) 509
RseI CAYNNNNRTG 5 cut(s) 62, 414, 842, 947, 1211
SatI GCNGC 6 cut(s) 108, 441, 700, 720, 853, 1549
Sau3AI GATC 9 cut(s) 364, 480, 645, 798, 807, 1243, 1385, 1398, 1479
Sau96I GGNCC 6 cut(s) 100, 464, 506, 524, 937, 1216
ScaI AGTACT 1 cut(s) 1250
SchI GAGTC 1 cut(s) 701
SduI GDGCHC 3 cut(s) 549, 1514, 1610
SfaNI GCATC 3 cut(s) 119, 1059, 1420
SinI GGWCC 4 cut(s) 464, 506, 937, 1216
SmaI CCCGGG 1 cut(s) 286
SmiMI CAYNNNNRTG 5 cut(s) 62, 414, 842, 947, 1211
SmlI CTYRAG 1 cut(s) 723
SmoI CTYRAG 1 cut(s) 723
SphI GCATGC 1 cut(s) 447
Sse9I AATT 5 cut(s) 263, 404, 773, 827, 1114
SsiI CCGC 2 cut(s) 16, 441
SspI AATATT 1 cut(s) 358
SspMI CTAG 2 cut(s) 746, 1458
StyI CCWWGG 3 cut(s) 57, 486, 520
TaaI ACNGT 3 cut(s) 143, 1018, 1256
TaiI ACGT 4 cut(s) 815, 864, 1329, 1491
TaqI TCGA 2 cut(s) 801, 1039
TaqII GACCGA 1 cut(s) 215
TasI AATT 5 cut(s) 263, 404, 773, 827, 1114
TatI WGTACW 2 cut(s) 921, 1248
TauI GCSGC 1 cut(s) 443
TfiI GAWTC 3 cut(s) 1102, 1149, 1191
TscAI CASTG 4 cut(s) 700, 892, 1102, 1489
TseI GCWGC 5 cut(s) 107, 699, 719, 852, 1548
TspDTI ATGAA 5 cut(s) 227, 398, 412, 612, 1094
TspGWI ACGGA 2 cut(s) 675, 724
TspMI CCCGGG 1 cut(s) 284
TspRI CASTG 4 cut(s) 700, 892, 1102, 1489
VpaK11BI GGWCC 4 cut(s) 464, 506, 937, 1216
XapI RAATTY 2 cut(s) 773, 827
XceI RCATGY 2 cut(s) 447, 847
XcmI CCANNNNNNNNNTGG 1 cut(s) 1470
XmaI CCCGGG 1 cut(s) 284
XmiI GTMKAC 2 cut(s) 307, 1197
XspI CTAG 2 cut(s) 746, 1458
ZrmI AGTACT 1 cut(s) 1250
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.