Rorug06G0243900

Exopolygalacturonase clone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0016557
Physical Location & Seq
Forward (+)
38827830 .. 38849463
21634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0243900.1

Sequence Viewer

Length: 903 bp
ATGGGCTTGCGTGCTGTGGCTGGATTTGTTAGATTGCTTTCAAAATCAGGTCCATGGTGGTTCCTGCATAGGGACACTGAGACATCTCAGGGATTGACCAAGCGCTTAAAGAAAGTTAGCAAGACGATCGATCAACTCCTGGAGAAGATAATAGACGAGCATGAACAAGTTGCCAACACTAGGAGTGGGAAACAAGGCCATGAGGACTTTGTAGACGTGTTGCTTTCGTTAACGAACCAACAATTGAACCCGAATGATGAGCAAGTACACTTTCTTGATCGAACAAATGTGAAAGCCATCTTACTAGACATGATCACCGCGGCTTTTGATACTTCGGCCACATCAATTGTTTGGAGCCTTGCCGAGCTCTTGAGGCATCCAAGGATCATGAAGAATCTCCAAGAAGAGCTCCAAAGTGTGGTTGGTATGGACCGAATGGTGGAAGAATATGATTTGCCCAAGCTGGATTACTTGAGTAAGGTGGTGAAGGAGAGCTTCAGACTACACCCAGTTGCACCGTTACTAGTCCCACATGAATCCATCGAGGACATTACAATCGATGGATATGACATTCCCAAGAAGTCGCGGATCATAGTAAACATCTGGAGCATTGGAAGAGATCCTAATGTTTGGTCCGAAAATGTTGAGGAACTTTATCCTAAAAGGTTCATGAACAACAACATAGACCTCCGGGGACATGACTTTCAGCTCATCCCATTTGGGTCTGGGCGCAGAGGGTGTCCGGGTATGCAATTAGGACTAACCACAGTTCAGCTAGTTCTGGCACAGCTGGTGCACTGCTTTAACTGGGAGCTCGATCCCAAATGGCTTGGTACCTCAAGACTTGGACATGTCCGAGGATTTCGGCTTATCACTGTCAAAAGACAAACACTTGCTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0016787 GO:0016798 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

300

Amino Acids

34.37

Weight (kDa)

7.16

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 10 - 275 2.5e-69 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016557)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g26010
malus_domestica MD03G1291400.v1.1 MD11G1306900.v1.1
prunus_persica Prupe.8G264500_v2.0.a1
pyrus_communis pycom03g23080 pycom11g26850
rosa_chinensis RchiOBHm_Chr6g0294381
rosa_laevigata RLG00000011876
rosa_multiflora Rmu_sc0005369.1_g000014
rosa_roxburghii Rroxscaffold_7G00173430
rosa_rugosa Rorug06G0243900
rosa_samantha Rh6AG356500 Rh6CG370500
rosa_wichuraiana Rw6G031070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 833
AccB1I GGYRCC 1 cut(s) 833
AccB7I CCANNNNNTGG 1 cut(s) 418
AccI GTMKAC 1 cut(s) 213
AccII CGCG 2 cut(s) 320, 586
AciI CCGC 3 cut(s) 318, 320, 586
AclWI GGATC 4 cut(s) 392, 596, 614, 812
AcoI YGGCCR 1 cut(s) 336
AcuI CTGAAG 1 cut(s) 481
AfaI GTAC 2 cut(s) 267, 835
AfeI AGCGCT 1 cut(s) 104
AfiI CCNNNNNNNGG 4 cut(s) 70, 180, 418, 439
AflIII ACRYGT 2 cut(s) 216, 850
AgsI TTSAA 2 cut(s) 42, 247
AhlI ACTAGT 1 cut(s) 523
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 8 cut(s) 367, 409, 463, 495, 709, 775, 790, 814
AluI AGCT 8 cut(s) 367, 409, 463, 495, 709, 775, 790, 814
Alw21I GWGCWC 4 cut(s) 369, 411, 798, 816
Alw26I GTCTC 1 cut(s) 74
Alw44I GTGCAC 1 cut(s) 794
AlwI GGATC 4 cut(s) 392, 596, 614, 812
Aor51HI AGCGCT 1 cut(s) 104
AoxI GGCC 2 cut(s) 196, 336
ApaLI GTGCAC 1 cut(s) 794
Asp718I GGTACC 1 cut(s) 833
AspLEI GCGC 2 cut(s) 105, 732
AspS9I GGNCC 3 cut(s) 50, 430, 633
AsuC2I CCSGG 2 cut(s) 692, 744
AsuHPI GGTGA 2 cut(s) 307, 496
AvaII GGWCC 3 cut(s) 50, 430, 633
BaeGI GKGCMC 1 cut(s) 798
BanI GGYRCC 1 cut(s) 833
BanII GRGCYC 3 cut(s) 369, 411, 816
Bbv12I GWGCWC 4 cut(s) 369, 411, 798, 816
BccI CCATC 3 cut(s) 305, 548, 554
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BciT130I CCWGG 1 cut(s) 140
BclI TGATCA 1 cut(s) 312
BcnI CCSGG 2 cut(s) 692, 744
BcoDI GTCTC 1 cut(s) 74
BcuI ACTAGT 1 cut(s) 523
BfaI CTAG 4 cut(s) 180, 305, 524, 776
BfoI RGCGCY 1 cut(s) 106
BisI GCNGC 1 cut(s) 321
BlsI GCNGC 1 cut(s) 322
Bme1390I CCNGG 3 cut(s) 140, 692, 744
Bme18I GGWCC 3 cut(s) 50, 430, 633
BmgBI CACGTC 1 cut(s) 217
BmgT120I GGNCC 3 cut(s) 50, 430, 633
BmiI GGNNCC 3 cut(s) 62, 356, 835
BmrFI CCNGG 3 cut(s) 140, 692, 744
BmrI ACTGGG 2 cut(s) 503, 817
BmsI GCATC 1 cut(s) 385
BmuI ACTGGG 2 cut(s) 503, 817
BpmI CTGGAG 2 cut(s) 161, 625
BpuEI CTTGAG 3 cut(s) 391, 493, 823
BpuMI CCSGG 2 cut(s) 692, 744
Bsa29I ATCGAT 2 cut(s) 129, 558
BsaJI CCNNGG 5 cut(s) 53, 318, 380, 691, 856
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 180, 418, 439
Bse1I ACTGG 2 cut(s) 509, 812
BseBI CCWGG 1 cut(s) 140
BseCI ATCGAT 2 cut(s) 129, 558
BseDI CCNNGG 5 cut(s) 53, 318, 380, 691, 856
BseGI GGATG 2 cut(s) 376, 711
BseLI CCNNNNNNNGG 4 cut(s) 70, 180, 418, 439
BseMII CTCAG 2 cut(s) 69, 101
BseNI ACTGG 2 cut(s) 509, 812
BseSI GKGCMC 1 cut(s) 798
Bsh1236I CGCG 2 cut(s) 320, 586
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 2 cut(s) 198, 338
BshNI GGYRCC 1 cut(s) 833
BshVI ATCGAT 2 cut(s) 129, 558
BsiEI CGRYCG 1 cut(s) 129
BsiHKAI GWGCWC 4 cut(s) 369, 411, 798, 816
BsiSI CCGG 2 cut(s) 691, 743
BslFI GGGAC 3 cut(s) 86, 512, 708
BslI CCNNNNNNNGG 4 cut(s) 70, 180, 418, 439
BsmAI GTCTC 1 cut(s) 74
BsmFI GGGAC 3 cut(s) 86, 512, 708
BsnI GGCC 2 cut(s) 198, 338
Bsp1286I GDGCHC 4 cut(s) 369, 411, 798, 816
Bsp143I GATC 8 cut(s) 126, 130, 277, 312, 384, 588, 619, 817
Bsp19I CCATGG 1 cut(s) 53
BspACI CCGC 3 cut(s) 318, 320, 586
BspANI GGCC 2 cut(s) 198, 338
BspCNI CTCAG 2 cut(s) 70, 100
BspDI ATCGAT 2 cut(s) 129, 558
BspFNI CGCG 2 cut(s) 320, 586
BspHI TCATGA 2 cut(s) 387, 669
BspLI GGNNCC 3 cut(s) 62, 356, 835
BspPI GGATC 4 cut(s) 392, 596, 614, 812
BspQI GCTCTTC 1 cut(s) 399
BspT107I GGYRCC 1 cut(s) 833
BsrI ACTGG 2 cut(s) 509, 812
BssECI CCNNGG 5 cut(s) 53, 318, 380, 691, 856
BssMI GATC 8 cut(s) 126, 130, 277, 312, 384, 588, 619, 817
BssT1I CCWWGG 2 cut(s) 53, 380
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 3 cut(s) 519, 769, 877
Bst6I CTCTTC 2 cut(s) 399, 610
BstC8I GCNNGC 3 cut(s) 8, 12, 898
BstDEI CTNAG 2 cut(s) 78, 87
BstDSI CCRYGG 2 cut(s) 53, 318
BstF5I GGATG 2 cut(s) 376, 711
BstFNI CGCG 2 cut(s) 320, 586
BstH2I RGCGCY 1 cut(s) 106
BstHHI GCGC 2 cut(s) 105, 732
BstKTI GATC 8 cut(s) 129, 133, 280, 315, 387, 591, 622, 820
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 8 cut(s) 126, 130, 277, 312, 384, 588, 619, 817
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 1 cut(s) 373
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 854
BstSCI CCNGG 3 cut(s) 138, 690, 742
BstSLI GKGCMC 1 cut(s) 798
BstUI CGCG 2 cut(s) 320, 586
BstX2I RGATCY 1 cut(s) 619
BstYI RGATCY 1 cut(s) 619
Bsu15I ATCGAT 2 cut(s) 129, 558
BsuRI GGCC 2 cut(s) 198, 338
BsuTUI ATCGAT 2 cut(s) 129, 558
BtgI CCRYGG 2 cut(s) 53, 318
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 2 cut(s) 376, 711
BtsI GCAGTG 1 cut(s) 796
BtsIMutI CAGTG 3 cut(s) 75, 796, 873
Cac8I GCNNGC 3 cut(s) 8, 12, 898
CciI TCATGA 2 cut(s) 387, 669
CfoI GCGC 2 cut(s) 105, 732
Cfr13I GGNCC 3 cut(s) 50, 430, 633
Cfr42I CCGCGG 1 cut(s) 321
ClaI ATCGAT 2 cut(s) 129, 558
Csp6I GTAC 2 cut(s) 266, 834
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 9 cut(s) 54, 161, 200, 310, 388, 533, 670, 698, 851
CviQI GTAC 2 cut(s) 266, 834
DdeI CTNAG 2 cut(s) 78, 87
DpnI GATC 8 cut(s) 128, 132, 279, 314, 386, 590, 621, 819
DpnII GATC 8 cut(s) 126, 130, 277, 312, 384, 588, 619, 817
EaeI YGGCCR 1 cut(s) 336
Eam1104I CTCTTC 2 cut(s) 399, 610
EarI CTCTTC 2 cut(s) 399, 610
Ecl136II GAGCTC 3 cut(s) 367, 409, 814
Eco130I CCWWGG 2 cut(s) 53, 380
Eco24I GRGCYC 3 cut(s) 369, 411, 816
Eco47I GGWCC 3 cut(s) 50, 430, 633
Eco47III AGCGCT 1 cut(s) 104
Eco53kI GAGCTC 3 cut(s) 367, 409, 814
Eco57I CTGAAG 1 cut(s) 481
EcoICRI GAGCTC 3 cut(s) 367, 409, 814
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 2 cut(s) 53, 380
EcoT38I GRGCYC 3 cut(s) 369, 411, 816
ErhI CCWWGG 2 cut(s) 53, 380
FaeI CATG 9 cut(s) 57, 164, 203, 313, 391, 536, 673, 701, 854
FalI AAGNNNNNCTT 2 cut(s) 479, 511
FaqI GGGAC 3 cut(s) 86, 512, 708
FatI CATG 9 cut(s) 53, 160, 199, 309, 387, 532, 669, 697, 850
FbaI TGATCA 1 cut(s) 312
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 1 cut(s) 321
FokI GGATG 2 cut(s) 363, 698
FriOI GRGCYC 3 cut(s) 369, 411, 816
Fsp4HI GCNGC 1 cut(s) 321
FspBI CTAG 4 cut(s) 180, 305, 524, 776
GlaI GCGC 2 cut(s) 104, 731
GluI GCNGC 1 cut(s) 321
GsuI CTGGAG 2 cut(s) 161, 625
HaeII RGCGCY 1 cut(s) 106
HaeIII GGCC 2 cut(s) 198, 338
HapII CCGG 2 cut(s) 691, 743
HhaI GCGC 2 cut(s) 105, 732
Hin1II CATG 9 cut(s) 57, 164, 203, 313, 391, 536, 673, 701, 854
Hin6I GCGC 2 cut(s) 103, 730
HinP1I GCGC 2 cut(s) 103, 730
HincII GTYRAC 1 cut(s) 231
HindII GTYRAC 1 cut(s) 231
HinfI GANTC 2 cut(s) 394, 536
HpaI GTTAAC 1 cut(s) 231
HpaII CCGG 2 cut(s) 691, 743
HphI GGTGA 2 cut(s) 307, 496
Hpy166II GTNNAC 5 cut(s) 214, 231, 268, 598, 796
Hpy188I TCNGA 3 cut(s) 500, 637, 857
Hpy188III TCNNGA 6 cut(s) 275, 370, 388, 604, 670, 840
Hpy8I GTNNAC 5 cut(s) 214, 231, 268, 598, 796
HpyAV CCTTC 1 cut(s) 481
HpyCH4III ACNGT 3 cut(s) 519, 769, 877
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 4 cut(s) 67, 515, 751, 796
HpyF10VI GCNNNNNNNGC 1 cut(s) 373
HpyF3I CTNAG 2 cut(s) 78, 87
HpySE526I ACGT 1 cut(s) 216
Hsp92II CATG 9 cut(s) 57, 164, 203, 313, 391, 536, 673, 701, 854
HspAI GCGC 2 cut(s) 103, 730
KpnI GGTACC 1 cut(s) 837
Ksp22I TGATCA 1 cut(s) 312
KspAI GTTAAC 1 cut(s) 231
KspI CCGCGG 1 cut(s) 321
Kzo9I GATC 8 cut(s) 126, 130, 277, 312, 384, 588, 619, 817
LguI GCTCTTC 1 cut(s) 399
LmnI GCTCC 4 cut(s) 354, 414, 606, 811
LweI GCATC 1 cut(s) 385
MaeI CTAG 4 cut(s) 180, 305, 524, 776
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 1 cut(s) 519
MalI GATC 8 cut(s) 128, 132, 279, 314, 386, 590, 621, 819
MboI GATC 8 cut(s) 126, 130, 277, 312, 384, 588, 619, 817
MboII GAAGA 5 cut(s) 157, 403, 416, 455, 627
MfeI CAATTG 2 cut(s) 242, 345
MflI RGATCY 1 cut(s) 619
MhlI GDGCHC 4 cut(s) 369, 411, 798, 816
MluCI AATT 3 cut(s) 242, 345, 752
MnlI CCTC 8 cut(s) 196, 366, 538, 640, 698, 728, 847, 851
MseI TTAA 3 cut(s) 107, 230, 804
MspA1I CMGCKG 2 cut(s) 320, 790
MspI CCGG 2 cut(s) 691, 743
MspR9I CCNGG 3 cut(s) 140, 692, 744
MunI CAATTG 2 cut(s) 242, 345
MvaI CCWGG 1 cut(s) 140
MvnI CGCG 2 cut(s) 320, 586
MwoI GCNNNNNNNGC 1 cut(s) 373
NciI CCSGG 2 cut(s) 692, 744
NcoI CCATGG 1 cut(s) 53
NdeII GATC 8 cut(s) 126, 130, 277, 312, 384, 588, 619, 817
NlaIII CATG 9 cut(s) 57, 164, 203, 313, 391, 536, 673, 701, 854
NlaIV GGNNCC 3 cut(s) 62, 356, 835
NmeAIII GCCGAG 1 cut(s) 388
NspI RCATGY 1 cut(s) 854
PagI TCATGA 2 cut(s) 387, 669
PciI ACATGT 1 cut(s) 850
PciSI GCTCTTC 1 cut(s) 399
PfeI GAWTC 2 cut(s) 394, 536
PflMI CCANNNNNTGG 1 cut(s) 418
PfoI TCCNGGA 1 cut(s) 138
PkrI GCNGC 1 cut(s) 322
Ple19I CGATCG 1 cut(s) 129
PscI ACATGT 1 cut(s) 850
Psp124BI GAGCTC 3 cut(s) 369, 411, 816
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 3 cut(s) 62, 356, 835
PspPI GGNCC 3 cut(s) 50, 430, 633
PsuI RGATCY 1 cut(s) 619
PvuI CGATCG 1 cut(s) 129
PvuII CAGCTG 1 cut(s) 790
RsaI GTAC 2 cut(s) 267, 835
RsaNI GTAC 2 cut(s) 266, 834
SacI GAGCTC 3 cut(s) 369, 411, 816
SacII CCGCGG 1 cut(s) 321
SapI GCTCTTC 1 cut(s) 399
SaqAI TTAA 3 cut(s) 107, 230, 804
SatI GCNGC 1 cut(s) 321
Sau3AI GATC 8 cut(s) 126, 130, 277, 312, 384, 588, 619, 817
Sau96I GGNCC 3 cut(s) 50, 430, 633
ScrFI CCNGG 3 cut(s) 140, 692, 744
SduI GDGCHC 4 cut(s) 369, 411, 798, 816
SfaNI GCATC 1 cut(s) 385
Sfr303I CCGCGG 1 cut(s) 321
SgrBI CCGCGG 1 cut(s) 321
SinI GGWCC 3 cut(s) 50, 430, 633
SmlI CTYRAG 3 cut(s) 370, 472, 838
SmoI CTYRAG 3 cut(s) 370, 472, 838
SpeI ACTAGT 1 cut(s) 523
Sse9I AATT 3 cut(s) 242, 345, 752
SsiI CCGC 3 cut(s) 318, 320, 586
SspMI CTAG 4 cut(s) 180, 305, 524, 776
SstI GAGCTC 3 cut(s) 369, 411, 816
StyD4I CCNGG 3 cut(s) 138, 690, 742
StyI CCWWGG 2 cut(s) 53, 380
TaaI ACNGT 3 cut(s) 519, 769, 877
TaiI ACGT 1 cut(s) 219
TaqI TCGA 5 cut(s) 129, 280, 543, 558, 816
TaqII GACCGA 1 cut(s) 447
TasI AATT 3 cut(s) 242, 345, 752
TatI WGTACW 1 cut(s) 265
TauI GCSGC 1 cut(s) 323
TfiI GAWTC 2 cut(s) 394, 536
Tru1I TTAA 3 cut(s) 107, 230, 804
Tru9I TTAA 3 cut(s) 107, 230, 804
TscAI CASTG 3 cut(s) 82, 803, 880
TspDTI ATGAA 5 cut(s) 177, 404, 549, 658, 686
TspRI CASTG 3 cut(s) 82, 803, 880
Van91I CCANNNNNTGG 1 cut(s) 418
VneI GTGCAC 1 cut(s) 794
VpaK11BI GGWCC 3 cut(s) 50, 430, 633
XceI RCATGY 1 cut(s) 854
XcmI CCANNNNNNNNNTGG 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 4 cut(s) 180, 305, 524, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.