3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G77680 |
| fragaria_vesca | FvH4_2g26510 |
| malus_domestica | MD08G1168200.v1.1 MD15G1353600.v1.1 |
| prunus_persica | Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 Prupe.1G501900_v2.0.a1 |
| pyrus_communis | pycom08g14370 pycom15g31690 pycom15g31710 |
| rosa_chinensis | RchiOBHm_Chr6g0295321 |
| rosa_laevigata | RLG00000011813 |
| rosa_multiflora | Rmu_sc0012530.1_g000002 Rmu_sc0016917.1_g000001 |
| rosa_roxburghii | Rroxscaffold_171G00441480 Rroxscaffold_7G00172580 |
| rosa_rugosa | Rorug06G0250000 |
| rosa_samantha | Rh6AG363200 Rh6BG371300 Rh6CG377800 Rh6DG363900 |
| rosa_wichuraiana | Rw6G031690 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 777 |
| AatII | GACGTC | 1 cut(s) | 501 |
| Acc16I | TGCGCA | 1 cut(s) | 2276 |
| Acc36I | ACCTGC | 1 cut(s) | 962 |
| AccB1I | GGYRCC | 2 cut(s) | 1507, 2256 |
| AccBSI | CCGCTC | 1 cut(s) | 509 |
| AccI | GTMKAC | 2 cut(s) | 2516, 2812 |
| AccII | CGCG | 3 cut(s) | 11, 76, 546 |
| AciI | CCGC | 7 cut(s) | 9, 11, 111, 507, 1867, 2207, 2251 |
| AclWI | GGATC | 6 cut(s) | 734, 747, 1220, 1545, 1562, 2120 |
| AcoI | YGGCCR | 3 cut(s) | 1864, 1984, 2805 |
| AcsI | RAATTY | 9 cut(s) | 347, 958, 1261, 1441, 1460, 1612, 2164, 2648, 2709 |
| AcuI | CTGAAG | 3 cut(s) | 1425, 2232, 2879 |
| AcyI | GRCGYC | 1 cut(s) | 498 |
| AfaI | GTAC | 6 cut(s) | 944, 1410, 1980, 3219, 3246, 3299 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1500, 1501, 1504 |
| AflIII | ACRYGT | 2 cut(s) | 2815, 3028 |
| AjnI | CCWGG | 4 cut(s) | 689, 1789, 1821, 3061 |
| AleI | CACNNNNGTG | 1 cut(s) | 3014 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2490, 2522 |
| Alw21I | GWGCWC | 3 cut(s) | 1908, 2077, 2245 |
| Alw26I | GTCTC | 2 cut(s) | 1795, 2637 |
| Alw44I | GTGCAC | 1 cut(s) | 2241 |
| AlwI | GGATC | 6 cut(s) | 734, 747, 1220, 1545, 1562, 2120 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 2210, 2359 |
| Ama87I | CYCGRG | 1 cut(s) | 1512 |
| AoxI | GGCC | 4 cut(s) | 6, 1864, 1984, 2805 |
| ApaLI | GTGCAC | 1 cut(s) | 2241 |
| ApeKI | GCWGC | 8 cut(s) | 389, 1328, 2437, 2576, 2693, 2729, 2732, 2780 |
| ApoI | RAATTY | 9 cut(s) | 347, 958, 1261, 1441, 1460, 1612, 2164, 2648, 2709 |
| AseI | ATTAAT | 3 cut(s) | 669, 1449, 2958 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 3145 |
| AspLEI | GCGC | 5 cut(s) | 713, 1737, 2277, 3042, 3079 |
| AspS9I | GGNCC | 6 cut(s) | 486, 687, 1249, 2850, 2906, 3272 |
| AsuC2I | CCSGG | 1 cut(s) | 408 |
| AsuHPI | GGTGA | 7 cut(s) | 59, 152, 396, 533, 1310, 1361, 1552 |
| AsuII | TTCGAA | 1 cut(s) | 1543 |
| AsuNHI | GCTAGC | 1 cut(s) | 2440 |
| AvaI | CYCGRG | 1 cut(s) | 1512 |
| AvaII | GGWCC | 6 cut(s) | 486, 687, 1249, 2850, 2906, 3272 |
| AxyI | CCTNAGG | 1 cut(s) | 1974 |
| BaeGI | GKGCMC | 1 cut(s) | 2245 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 1733, 1766, 2528, 2561 |
| BalI | TGGCCA | 2 cut(s) | 1986, 2807 |
| BamHI | GGATCC | 1 cut(s) | 739 |
| BanI | GGYRCC | 2 cut(s) | 1507, 2256 |
| BanII | GRGCYC | 1 cut(s) | 3198 |
| BauI | CACGAG | 1 cut(s) | 1332 |
| BbsI | GAAGAC | 2 cut(s) | 1478, 3215 |
| Bbv12I | GWGCWC | 3 cut(s) | 1908, 2077, 2245 |
| BbvCI | CCTCAGC | 1 cut(s) | 2592 |
| BbvI | GCAGC | 8 cut(s) | 376, 1315, 2449, 2563, 2680, 2716, 2744, 2767 |
| BccI | CCATC | 9 cut(s) | 38, 347, 1034, 1851, 1919, 1989, 2063, 2358, 2977 |
| BceAI | ACGGC | 2 cut(s) | 8, 1981 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 102, 136, 638, 672, 1533, 1567 |
| BciT130I | CCWGG | 4 cut(s) | 691, 1791, 1823, 3063 |
| BciVI | GTATCC | 2 cut(s) | 2320, 2556 |
| BclI | TGATCA | 1 cut(s) | 925 |
| BcnI | CCSGG | 1 cut(s) | 408 |
| BcoDI | GTCTC | 2 cut(s) | 1795, 2637 |
| BfaI | CTAG | 8 cut(s) | 512, 1707, 2223, 2441, 2573, 2703, 2910, 2996 |
| BfmI | CTRYAG | 3 cut(s) | 597, 2730, 3240 |
| BfoI | RGCGCY | 3 cut(s) | 714, 3043, 3080 |
| BfuAI | ACCTGC | 1 cut(s) | 962 |
| BfuI | GTATCC | 2 cut(s) | 2320, 2556 |
| BglII | AGATCT | 4 cut(s) | 1585, 2012, 2034, 2060 |
| BmcAI | AGTACT | 1 cut(s) | 3246 |
| Bme1390I | CCNGG | 5 cut(s) | 408, 691, 1791, 1823, 3063 |
| Bme18I | GGWCC | 6 cut(s) | 486, 687, 1249, 2850, 2906, 3272 |
| BmeT110I | CYCGRG | 1 cut(s) | 1512 |
| BmgT120I | GGNCC | 6 cut(s) | 486, 687, 1249, 2850, 2906, 3272 |
| BmiI | GGNNCC | 6 cut(s) | 430, 741, 1509, 2258, 2852, 2907 |
| BmrFI | CCNGG | 5 cut(s) | 408, 691, 1791, 1823, 3063 |
| BmtI | GCTAGC | 1 cut(s) | 2444 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1968 |
| BpiI | GAAGAC | 2 cut(s) | 1478, 3215 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 3146, 3178 |
| BpmI | CTGGAG | 6 cut(s) | 990, 1615, 1860, 2063, 2381, 2825 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1911, 2592 |
| Bpu14I | TTCGAA | 1 cut(s) | 1543 |
| BpuEI | CTTGAG | 1 cut(s) | 2366 |
| BpuMI | CCSGG | 1 cut(s) | 408 |
| Bsa29I | ATCGAT | 1 cut(s) | 1855 |
| BsaAI | YACGTR | 1 cut(s) | 3029 |
| BsaHI | GRCGYC | 1 cut(s) | 498 |
| BsaJI | CCNNGG | 7 cut(s) | 9, 489, 795, 1499, 1511, 1686, 3198 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1500, 1501, 1504 |
| Bse118I | RCCGGY | 1 cut(s) | 2541 |
| Bse21I | CCTNAGG | 1 cut(s) | 1974 |
| Bse3DI | GCAATG | 2 cut(s) | 1647, 2797 |
| BseBI | CCWGG | 4 cut(s) | 691, 1791, 1823, 3063 |
| BseCI | ATCGAT | 1 cut(s) | 1855 |
| BseDI | CCNNGG | 7 cut(s) | 9, 489, 795, 1499, 1511, 1686, 3198 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1500, 1501, 1504 |
| BseMI | GCAATG | 2 cut(s) | 1647, 2797 |
| BseMII | CTCAG | 8 cut(s) | 144, 473, 837, 1617, 1925, 1988, 2583, 2895 |
| BseRI | GAGGAG | 5 cut(s) | 91, 94, 2071, 2192, 2609 |
| BseSI | GKGCMC | 1 cut(s) | 2245 |
| BseXI | GCAGC | 8 cut(s) | 376, 1315, 2449, 2563, 2680, 2716, 2744, 2767 |
| BseYI | CCCAGC | 1 cut(s) | 2753 |
| BsgI | GTGCAG | 2 cut(s) | 711, 1314 |
| Bsh1236I | CGCG | 3 cut(s) | 11, 76, 546 |
| BshFI | GGCC | 4 cut(s) | 8, 1866, 1986, 2807 |
| BshNI | GGYRCC | 2 cut(s) | 1507, 2256 |
| BshVI | ATCGAT | 1 cut(s) | 1855 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1908, 2077, 2245 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1512 |
| BsiSI | CCGG | 2 cut(s) | 408, 2542 |
| BslFI | GGGAC | 5 cut(s) | 184, 210, 303, 2836, 2919 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1500, 1501, 1504 |
| BsmAI | GTCTC | 2 cut(s) | 1795, 2637 |
| BsmFI | GGGAC | 5 cut(s) | 184, 210, 303, 2836, 2919 |
| BsnI | GGCC | 4 cut(s) | 8, 1866, 1986, 2807 |
| BsoBI | CYCGRG | 1 cut(s) | 1512 |
| Bsp119I | TTCGAA | 1 cut(s) | 1543 |
| Bsp1286I | GDGCHC | 4 cut(s) | 1908, 2077, 2245, 3198 |
| Bsp1407I | TGTACA | 1 cut(s) | 3297 |
| Bsp19I | CCATGG | 3 cut(s) | 489, 1499, 3198 |
| BspACI | CCGC | 7 cut(s) | 9, 11, 111, 507, 1867, 2207, 2251 |
| BspANI | GGCC | 4 cut(s) | 8, 1866, 1986, 2807 |
| BspCNI | CTCAG | 8 cut(s) | 145, 472, 838, 1616, 1924, 1987, 2584, 2894 |
| BspDI | ATCGAT | 1 cut(s) | 1855 |
| BspFNI | CGCG | 3 cut(s) | 11, 76, 546 |
| BspHI | TCATGA | 2 cut(s) | 862, 2412 |
| BspLI | GGNNCC | 6 cut(s) | 430, 741, 1509, 2258, 2852, 2907 |
| BspMAI | CTGCAG | 1 cut(s) | 2734 |
| BspMI | ACCTGC | 1 cut(s) | 962 |
| BspOI | GCTAGC | 1 cut(s) | 2444 |
| BspPI | GGATC | 6 cut(s) | 734, 747, 1220, 1545, 1562, 2120 |
| BspT104I | TTCGAA | 1 cut(s) | 1543 |
| BspT107I | GGYRCC | 2 cut(s) | 1507, 2256 |
| BsrBI | CCGCTC | 1 cut(s) | 509 |
| BsrDI | GCAATG | 2 cut(s) | 1647, 2797 |
| BsrFI | RCCGGY | 1 cut(s) | 2541 |
| BsrGI | TGTACA | 1 cut(s) | 3297 |
| BssAI | RCCGGY | 1 cut(s) | 2541 |
| BssECI | CCNNGG | 7 cut(s) | 9, 489, 795, 1499, 1511, 1686, 3198 |
| BssNAI | GTATAC | 1 cut(s) | 2517 |
| BssNI | GRCGYC | 1 cut(s) | 498 |
| BssSI | CACGAG | 1 cut(s) | 1332 |
| BssT1I | CCWWGG | 5 cut(s) | 489, 795, 1499, 1686, 3198 |
| Bst1107I | GTATAC | 1 cut(s) | 2517 |
| Bst2BI | CACGAG | 1 cut(s) | 1332 |
| Bst2UI | CCWGG | 4 cut(s) | 691, 1791, 1823, 3063 |
| Bst4CI | ACNGT | 6 cut(s) | 452, 769, 1625, 1873, 2240, 3217 |
| Bst6I | CTCTTC | 1 cut(s) | 441 |
| BstACI | GRCGYC | 1 cut(s) | 498 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 244 |
| BstAUI | TGTACA | 1 cut(s) | 3297 |
| BstBAI | YACGTR | 1 cut(s) | 3029 |
| BstBI | TTCGAA | 1 cut(s) | 1543 |
| BstC8I | GCNNGC | 9 cut(s) | 74, 709, 1892, 1908, 2279, 2442, 2769, 2805, 2940 |
| BstDSI | CCRYGG | 4 cut(s) | 9, 489, 1499, 3198 |
| BstEII | GGTNACC | 1 cut(s) | 402 |
| BstFNI | CGCG | 3 cut(s) | 11, 76, 546 |
| BstH2I | RGCGCY | 3 cut(s) | 714, 3043, 3080 |
| BstHHI | GCGC | 5 cut(s) | 713, 1737, 2277, 3042, 3079 |
| BstMAI | GTCTC | 2 cut(s) | 1795, 2637 |
| BstNI | CCWGG | 4 cut(s) | 691, 1791, 1823, 3063 |
| BstNSI | RCATGY | 3 cut(s) | 877, 1388, 2819 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1968 |
| BstPI | GGTNACC | 1 cut(s) | 402 |
| BstSCI | CCNGG | 5 cut(s) | 406, 689, 1789, 1821, 3061 |
| BstSFI | CTRYAG | 3 cut(s) | 597, 2730, 3240 |
| BstSLI | GKGCMC | 1 cut(s) | 2245 |
| BstUI | CGCG | 3 cut(s) | 11, 76, 546 |
| BstV1I | GCAGC | 8 cut(s) | 376, 1315, 2449, 2563, 2680, 2716, 2744, 2767 |
| BstV2I | GAAGAC | 2 cut(s) | 1478, 3215 |
| BstX2I | RGATCY | 7 cut(s) | 739, 1537, 1585, 2012, 2034, 2060, 2125 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1588 |
| BstYI | RGATCY | 7 cut(s) | 739, 1537, 1585, 2012, 2034, 2060, 2125 |
| BstZ17I | GTATAC | 1 cut(s) | 2517 |
| Bsu15I | ATCGAT | 1 cut(s) | 1855 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1974 |
| BsuI | GTATCC | 2 cut(s) | 2320, 2556 |
| BsuRI | GGCC | 4 cut(s) | 8, 1866, 1986, 2807 |
| BsuTUI | ATCGAT | 1 cut(s) | 1855 |
| BtgI | CCRYGG | 4 cut(s) | 9, 489, 1499, 3198 |
| BtgZI | GCGATG | 2 cut(s) | 2549, 2750 |
| BtsI | GCAGTG | 1 cut(s) | 1575 |
| BtsIMutI | CAGTG | 7 cut(s) | 481, 738, 1575, 1869, 2245, 2357, 2667 |
| BveI | ACCTGC | 1 cut(s) | 962 |
| Cac8I | GCNNGC | 9 cut(s) | 74, 709, 1892, 1908, 2279, 2442, 2769, 2805, 2940 |
| CaiI | CAGNNNCTG | 2 cut(s) | 2210, 2359 |
| CciI | TCATGA | 2 cut(s) | 862, 2412 |
| CfoI | GCGC | 5 cut(s) | 713, 1737, 2277, 3042, 3079 |
| Cfr10I | RCCGGY | 1 cut(s) | 2541 |
| Cfr13I | GGNCC | 6 cut(s) | 486, 687, 1249, 2850, 2906, 3272 |
| Cfr42I | CCGCGG | 1 cut(s) | 12 |
| ClaI | ATCGAT | 1 cut(s) | 1855 |
| CseI | GACGC | 3 cut(s) | 56, 135, 535 |
| Csp6I | GTAC | 6 cut(s) | 943, 1409, 1979, 3218, 3245, 3298 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1227, 1262 |
| CviQI | GTAC | 6 cut(s) | 943, 1409, 1979, 3218, 3245, 3298 |
| EaeI | YGGCCR | 3 cut(s) | 1864, 1984, 2805 |
| Eam1104I | CTCTTC | 1 cut(s) | 441 |
| EarI | CTCTTC | 1 cut(s) | 441 |
| Eco130I | CCWWGG | 5 cut(s) | 489, 795, 1499, 1686, 3198 |
| Eco24I | GRGCYC | 1 cut(s) | 3198 |
| Eco47I | GGWCC | 6 cut(s) | 486, 687, 1249, 2850, 2906, 3272 |
| Eco57I | CTGAAG | 3 cut(s) | 1425, 2232, 2879 |
| Eco81I | CCTNAGG | 1 cut(s) | 1974 |
| Eco88I | CYCGRG | 1 cut(s) | 1512 |
| Eco91I | GGTNACC | 1 cut(s) | 402 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2906 |
| EcoO65I | GGTNACC | 1 cut(s) | 402 |
| EcoRII | CCWGG | 4 cut(s) | 689, 1789, 1821, 3061 |
| EcoT14I | CCWWGG | 5 cut(s) | 489, 795, 1499, 1686, 3198 |
| EcoT38I | GRGCYC | 1 cut(s) | 3198 |
| ErhI | CCWWGG | 5 cut(s) | 489, 795, 1499, 1686, 3198 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1344, 1376 |
| FaqI | GGGAC | 5 cut(s) | 184, 210, 303, 2836, 2919 |
| FauI | CCCGC | 1 cut(s) | 118 |
| FauNDI | CATATG | 1 cut(s) | 2131 |
| FbaI | TGATCA | 1 cut(s) | 925 |
| FblI | GTMKAC | 2 cut(s) | 2516, 2812 |
| FriOI | GRGCYC | 1 cut(s) | 3198 |
| FspBI | CTAG | 8 cut(s) | 512, 1707, 2223, 2441, 2573, 2703, 2910, 2996 |
| FspI | TGCGCA | 1 cut(s) | 2276 |
| GlaI | GCGC | 5 cut(s) | 712, 1736, 2276, 3041, 3078 |
| GsaI | CCCAGC | 1 cut(s) | 2757 |
| GsuI | CTGGAG | 6 cut(s) | 990, 1615, 1860, 2063, 2381, 2825 |
| HaeII | RGCGCY | 3 cut(s) | 714, 3043, 3080 |
| HaeIII | GGCC | 4 cut(s) | 8, 1866, 1986, 2807 |
| HapII | CCGG | 2 cut(s) | 408, 2542 |
| HgaI | GACGC | 3 cut(s) | 56, 135, 535 |
| HhaI | GCGC | 5 cut(s) | 713, 1737, 2277, 3042, 3079 |
| Hin1I | GRCGYC | 1 cut(s) | 498 |
| Hin6I | GCGC | 5 cut(s) | 711, 1735, 2275, 3040, 3077 |
| HinP1I | GCGC | 5 cut(s) | 711, 1735, 2275, 3040, 3077 |
| HincII | GTYRAC | 1 cut(s) | 31 |
| HindII | GTYRAC | 1 cut(s) | 31 |
| HindIII | AAGCTT | 3 cut(s) | 1892, 2279, 2936 |
| HpaII | CCGG | 2 cut(s) | 408, 2542 |
| HphI | GGTGA | 7 cut(s) | 59, 152, 396, 533, 1310, 1361, 1552 |
| Hpy166II | GTNNAC | 5 cut(s) | 31, 1645, 2243, 2517, 2813 |
| Hpy8I | GTNNAC | 5 cut(s) | 31, 1645, 2243, 2517, 2813 |
| Hpy99I | CGWCG | 5 cut(s) | 29, 72, 151, 1098, 2955 |
| HpyCH4III | ACNGT | 6 cut(s) | 452, 769, 1625, 1873, 2240, 3217 |
| HpyCH4IV | ACGT | 5 cut(s) | 498, 1051, 2950, 3028, 3220 |
| HpySE526I | ACGT | 5 cut(s) | 498, 1051, 2950, 3028, 3220 |
| Hsp92I | GRCGYC | 1 cut(s) | 498 |
| HspAI | GCGC | 5 cut(s) | 711, 1735, 2275, 3040, 3077 |
| Ksp22I | TGATCA | 1 cut(s) | 925 |
| KspI | CCGCGG | 1 cut(s) | 12 |
| LmnI | GCTCC | 8 cut(s) | 342, 625, 1318, 1369, 1400, 2362, 2844, 3167 |
| Lsp1109I | GCAGC | 8 cut(s) | 376, 1315, 2449, 2563, 2680, 2716, 2744, 2767 |
| MaeI | CTAG | 8 cut(s) | 512, 1707, 2223, 2441, 2573, 2703, 2910, 2996 |
| MaeII | ACGT | 5 cut(s) | 498, 1051, 2950, 3028, 3220 |
| MaeIII | GTNAC | 6 cut(s) | 187, 402, 884, 1671, 2355, 2489 |
| MbiI | CCGCTC | 1 cut(s) | 509 |
| MfeI | CAATTG | 2 cut(s) | 947, 1308 |
| MflI | RGATCY | 7 cut(s) | 739, 1537, 1585, 2012, 2034, 2060, 2125 |
| MhlI | GDGCHC | 4 cut(s) | 1908, 2077, 2245, 3198 |
| MlsI | TGGCCA | 2 cut(s) | 1986, 2807 |
| MluNI | TGGCCA | 2 cut(s) | 1986, 2807 |
| MlyI | GAGTC | 2 cut(s) | 232, 881 |
| MmeI | TCCRAC | 6 cut(s) | 889, 1079, 1644, 1980, 2304, 3118 |
| Mox20I | TGGCCA | 2 cut(s) | 1986, 2807 |
| MroXI | GAANNNNTTC | 1 cut(s) | 3145 |
| MscI | TGGCCA | 2 cut(s) | 1986, 2807 |
| MslI | CAYNNNNRTG | 1 cut(s) | 3014 |
| Msp20I | TGGCCA | 2 cut(s) | 1986, 2807 |
| MspA1I | CMGCKG | 4 cut(s) | 11, 389, 2207, 2729 |
| MspI | CCGG | 2 cut(s) | 408, 2542 |
| MspR9I | CCNGG | 5 cut(s) | 408, 691, 1791, 1823, 3063 |
| MunI | CAATTG | 2 cut(s) | 947, 1308 |
| MvaI | CCWGG | 4 cut(s) | 691, 1791, 1823, 3063 |
| MvnI | CGCG | 3 cut(s) | 11, 76, 546 |
| NciI | CCSGG | 1 cut(s) | 408 |
| NcoI | CCATGG | 3 cut(s) | 489, 1499, 3198 |
| NdeI | CATATG | 1 cut(s) | 2131 |
| NheI | GCTAGC | 1 cut(s) | 2440 |
| NlaIV | GGNNCC | 6 cut(s) | 430, 741, 1509, 2258, 2852, 2907 |
| NmuCI | GTSAC | 5 cut(s) | 187, 402, 884, 2355, 2489 |
| NsbI | TGCGCA | 1 cut(s) | 2276 |
| NspI | RCATGY | 3 cut(s) | 877, 1388, 2819 |
| NspV | TTCGAA | 1 cut(s) | 1543 |
| OliI | CACNNNNGTG | 1 cut(s) | 3014 |
| PagI | TCATGA | 2 cut(s) | 862, 2412 |
| PciI | ACATGT | 1 cut(s) | 2815 |
| PdmI | GAANNNNTTC | 1 cut(s) | 3145 |
| PfoI | TCCNGGA | 2 cut(s) | 1789, 1821 |
| PleI | GAGTC | 2 cut(s) | 231, 881 |
| PpsI | GAGTC | 2 cut(s) | 231, 881 |
| Ppu21I | YACGTR | 1 cut(s) | 3029 |
| PpuMI | RGGWCCY | 1 cut(s) | 2906 |
| PscI | ACATGT | 1 cut(s) | 2815 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1968 |
| PshBI | ATTAAT | 3 cut(s) | 669, 1449, 2958 |
| PsiI | TTATAA | 1 cut(s) | 777 |
| Psp5II | RGGWCCY | 1 cut(s) | 2906 |
| Psp6I | CCWGG | 4 cut(s) | 689, 1789, 1821, 3061 |
| PspEI | GGTNACC | 1 cut(s) | 402 |
| PspFI | CCCAGC | 1 cut(s) | 2753 |
| PspGI | CCWGG | 4 cut(s) | 689, 1789, 1821, 3061 |
| PspN4I | GGNNCC | 6 cut(s) | 430, 741, 1509, 2258, 2852, 2907 |
| PspPI | GGNCC | 6 cut(s) | 486, 687, 1249, 2850, 2906, 3272 |
| PspPPI | RGGWCCY | 1 cut(s) | 2906 |
| PstI | CTGCAG | 1 cut(s) | 2734 |
| PstNI | CAGNNNCTG | 2 cut(s) | 2210, 2359 |
| PsuI | RGATCY | 7 cut(s) | 739, 1537, 1585, 2012, 2034, 2060, 2125 |
| PvuII | CAGCTG | 2 cut(s) | 389, 2729 |
| RsaI | GTAC | 6 cut(s) | 944, 1410, 1980, 3219, 3246, 3299 |
| RsaNI | GTAC | 6 cut(s) | 943, 1409, 1979, 3218, 3245, 3298 |
| RseI | CAYNNNNRTG | 1 cut(s) | 3014 |
| SacII | CCGCGG | 1 cut(s) | 12 |
| Sau96I | GGNCC | 6 cut(s) | 486, 687, 1249, 2850, 2906, 3272 |
| ScaI | AGTACT | 1 cut(s) | 3246 |
| SchI | GAGTC | 2 cut(s) | 232, 881 |
| ScrFI | CCNGG | 5 cut(s) | 408, 691, 1791, 1823, 3063 |
| SduI | GDGCHC | 4 cut(s) | 1908, 2077, 2245, 3198 |
| SfcI | CTRYAG | 3 cut(s) | 597, 2730, 3240 |
| Sfr303I | CCGCGG | 1 cut(s) | 12 |
| SfuI | TTCGAA | 1 cut(s) | 1543 |
| SgrBI | CCGCGG | 1 cut(s) | 12 |
| SinI | GGWCC | 6 cut(s) | 486, 687, 1249, 2850, 2906, 3272 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 3014 |
| SmlI | CTYRAG | 1 cut(s) | 2381 |
| SmoI | CTYRAG | 1 cut(s) | 2381 |
| SsiI | CCGC | 7 cut(s) | 9, 11, 111, 507, 1867, 2207, 2251 |
| SspI | AATATT | 3 cut(s) | 1380, 1774, 1818 |
| SspMI | CTAG | 8 cut(s) | 512, 1707, 2223, 2441, 2573, 2703, 2910, 2996 |
| StyD4I | CCNGG | 5 cut(s) | 406, 689, 1789, 1821, 3061 |
| StyI | CCWWGG | 5 cut(s) | 489, 795, 1499, 1686, 3198 |
| TaaI | ACNGT | 6 cut(s) | 452, 769, 1625, 1873, 2240, 3217 |
| TaiI | ACGT | 5 cut(s) | 501, 1054, 2953, 3031, 3223 |
| TaqI | TCGA | 7 cut(s) | 89, 1011, 1543, 1855, 2344, 2953, 3117 |
| TatI | WGTACW | 3 cut(s) | 1408, 3244, 3297 |
| TauI | GCSGC | 4 cut(s) | 11, 509, 1869, 2210 |
| TscAI | CASTG | 7 cut(s) | 488, 738, 1582, 1876, 2245, 2364, 2674 |
| TseFI | GTSAC | 5 cut(s) | 187, 402, 884, 2355, 2489 |
| TseI | GCWGC | 8 cut(s) | 389, 1328, 2437, 2576, 2693, 2729, 2732, 2780 |
| Tsp45I | GTSAC | 5 cut(s) | 187, 402, 884, 2355, 2489 |
| TspGWI | ACGGA | 2 cut(s) | 638, 3285 |
| TspRI | CASTG | 7 cut(s) | 488, 738, 1582, 1876, 2245, 2364, 2674 |
| VneI | GTGCAC | 1 cut(s) | 2241 |
| VpaK11BI | GGWCC | 6 cut(s) | 486, 687, 1249, 2850, 2906, 3272 |
| VspI | ATTAAT | 3 cut(s) | 669, 1449, 2958 |
| XapI | RAATTY | 9 cut(s) | 347, 958, 1261, 1441, 1460, 1612, 2164, 2648, 2709 |
| XbaI | TCTAGA | 3 cut(s) | 511, 1706, 2222 |
| XceI | RCATGY | 3 cut(s) | 877, 1388, 2819 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 681, 687 |
| XmiI | GTMKAC | 2 cut(s) | 2516, 2812 |
| XmnI | GAANNNNTTC | 1 cut(s) | 3145 |
| XspI | CTAG | 8 cut(s) | 512, 1707, 2223, 2441, 2573, 2703, 2910, 2996 |
| ZraI | GACGTC | 1 cut(s) | 499 |
| ZrmI | AGTACT | 1 cut(s) | 3246 |