MD15G1353600.v1.1

3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007261
Physical Location & Seq
Reverse (-)
42349002 .. 42354663
5662 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1353600.v1.1.491

Sequence Viewer

Length: 3339 bp
ATGAAAGGCGCGGTTGTTCAATCGGTTGTTGAAAGGGTTGAAGACGGCGATAAGGAGAAGAAGAAGAGGCGCAGATCCAATCGCCGATCCAAGCAAAACAATTCCACTTCAGCTAGTCCGATAAATGAAATACGAGGTGAAGTATTTGAGTGCCTGGCAAATGGAAGTATGCCTCATCATGTAAACACATCCTTGAAGCAACTTCAGTTAGACATGCATTCTCCATATGAGCACGGAATGATCAAAGCATCTAATTTTGCCTTCAATTCATTGCCAACAATGCACATCAATGAACAAGTCAACCACGAGGATGTGCAAATTTTGGTATATCAGCACTCTCTCCAATGTGATCCTTCTGAAAGAGTTATTGCAAATTCTTGTCCTAAACCCTTGGCTTGTAGAGGATCACCTGCAATGTTCAAGGATTTCCCTATTCATAACATTGAGAGTCATGCTCGGAGAAAATGTTTTACTTCACACTGGCCCATAGAGGCTGTTAATGATGCTCTGGAGAAAGGTGATGCTTTCAGAGCTGTGTTTCGAGTCAATGCACACAACCGACTTGAGGCCTATTGCAATATTGATGGTGTACCAACGGATGTTCTCGTTGGAGGACATGCTGAGCAGAATAGAGCTGTGGAAGGAGACATTGTGGCGATTAAGGTTGATCCCTTAGTGTTATGGACTAGGATGAAAGGATCAGCTGGGACTTGTACCAGCTCTGCACTGGTGGAGGATTTTAACTTACCACAAGAAGCTAATGAAATTTCTGGTCTTAACTGCAAAGGTAAAGATAAGGTAGACGAAGTGAATTTGTATGATAATGATAGAAGAAGCCTGTTTCCTGAGAGGGGCTCACATCCTGAAGAAAGTAACACCCTTGGTGAATCTGTTAATTCAGAGCGAATTGGACAAACAAGTTATGATCATATGACTGGAAGGTACCCCTCGGCTTCAGATTCTTTACGAGCAGGTTCTCATGGTGAGCAGAATGAAGTTGCAGTTGCGGTAGAGTGGATGTGTGCCACGATCAGTTCATTCCCTTCAAAACGACCAACTGGCAGGGTTGTTGCTATCATTGAAAGGTCTCCTCGTCGAGACGCTGTTGTTGGTTTTCTTCATGTTAAGAAGTGGATTGCTTACAGGGAGGTTTGCAGAAACGACACGAGAAAGAACAAAAATGGATCATTTTCTAACAACGAGTACATCCAGATGACCCCAACTGACCCCAGATTTCCAAAAATGGTGGTCCTTGTGAGAACCCTGCCTGATTCGATCAAGAAAAGATTGGAGAATGGTGATGAGTCAATCGAAATGGAATTGTTTGCTGCCCGAATAGATGAATGGGATGAACAAAGTTCTGCTCCTCAAGCTGTCATCTTGAATGCTTTTGGGCGGGCAGGTGAAGTACAGCCACAAGTTGAAGCAATTTTATTCCAAAATTCAATTAATTGGTCTGATTTTTGTCATGAATCACTTTCCTGTCTTCCTCCTCTCCCATGGGAGGTGCCTCAGGAGGAAATCAAAAGTAGAAAGGATCTTCGAAACCTGTTCATATTTACCATCGACCCTTCCACTGCTACTGATCTGGATGATGCTCTCTCAATTGAGAAATTACCCAATGGAATTTTCAGAGTTGGTATCCACATTGCTGATGTGTCGTATTTTGTTTTGCCTGACACACCCTTAGACAAAGAAGCTCAATCTAGATCAACAAGTGTGTATATGTCACAGAGGAAATTACCAATGTTGCCTCCTTTGCTCTCAGAGGATATTGGTTCGCTTAACCCAGGAGTAGAGAGACTTGCATTTTCAATTTTCTTTGACATCAACCATGTTGGGGATGTTGTTGACCGATGGATTGGCCGCACTGTAATAAGGTCTTGTTGCAAGCTATCTTATGAACATGCTCAGGAGATTATCAATGGGAAACTTAATTTGGAAAGTTCTAACATTTTAGGACATGGTCGTCCTCAGTTGCATGGCCATTTTGAATGGTCTGATGTAATTAGATCTGTCAATGACCTTCATGAAATTTCCAGAGTTTTGAAGGAGAGGAGGTTTAGTGATGGGGCTCTTCAGCTTGAAAGCTCCAAAGTTGTTATTCTGTTTGACGAAAATGGAGTTCCATATGATAGCATGTATTCTGAATGGAAGGACTCCAATTTTCTTGTCGAGGAGTTTATGCTTTTAGCAAATAGAGCAGCGGCTGAAGTCATATCTAGAGCTTTTCCTGAGAATGCATTACTGCGGAGGCACCCAGAACCTACTATGCGCAAACTCAGGGAATTTGAAGCATTTTGTTCTAAGCATGGTTTGGAATTGGATACATCTTCTTCTGGGCAGTTCCAGCAATCATTGCAGAGAATCAGGGAAGAGCTCAAGGATGACTCTGTTCTATTCAATATTCTTATGAATTATGCTACAAAACCAATGCAGTTGGCTTCTTATTTTTGTAGTGGCCAATTAAAAGATAGAGAAAATGATTGGGGTCACTATGGACTTGCTGTTCCTTTGTACACTCATTTTACGTCACCACTGCGTCGGTATCCAGATATTGTGGTGCATCGCACATTGGCTGCAGTTATAGAGGCAGAGGAGTTATTTTTGAAATGTCGGAGAATATTGAATAATTTAAGCAGGGGAGATGAAGTTAAAATGAGATGTTTCACTGGTATTTATTTTGATAAGGATGTTGCTGAATCCTGTGAAATCAAGGAAGCATTATTGGCAGCAGCAATAAAGCAAAGAGTTCCCTGCTCTGAAATGCTTGCTGATGTTGCCGCTTATTGCAATGAAAGAAAGCTGGCTAGTAGACATGTCAAAGATGCTTGTGATAAGCTATACATGTGGGCCCTGCTGAAGAAAAAGGAGTTGCAGATTGTCCTGTCAGAAGCAAGAGTATTGGGTGTAGGACCTAGGTTCATGTCCATTTATATTCACAAGCTTGCTGTTGAACGTCGAATATATTATGATGAAGTTGAAGGCTTGATGGCAGAATGGCTTGATGCGACGTCCACAGTGGTGCTGAATTTATGTTCTAACAGACGCTCGCTTAGGAGAGGTAGTCCTGGTAAATGGAGGGCACTTGAAGATGTTGCCTTGGTTGTAAGACCTTATGGCCTCAAAGCTGAACTAGGCGGAACTGGAAATAGTTCTAATGAGGGTGCTGGGACCCAAGATGTTGGCGGAGCCACCCATAGTGAAATCGATCCTTTGGTTTTCCCCCTTACACTGCGTGTTCTTTCAACCATTCCTGTAGTGCTTCATGCAGTTGGAGGGGATGATGGACCCGTCGATATTGGGGCAAGGCTGTACATGAGCTCATATCTCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000175 GO:0000178 GO:0000278 GO:0000280 GO:0000287 GO:0000291 GO:0000819 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005844 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0008285 GO:0008408 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010586 GO:0010587 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019222 GO:0019439 GO:0019827 GO:0022402 GO:0022613 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035770 GO:0036464 GO:0042127 GO:0042254 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0046872 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051276 GO:0051304 GO:0051306 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0097159 GO:0098727 GO:0098813 GO:0140014 GO:0140098 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1902494 GO:1903047 GO:1905354 GO:1990074 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNB

Protein Analysis

1113

Amino Acids

125.15

Weight (kDa)

6.08

Isoelectric Point (pI)

54.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Dis3 PF17849 402 - 481 4.5e-16 Dis3-like cold-shock domain 2 (CSD2)
RNB PF00773 511 - 864 1.8e-102 RNB domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 418, 1391
AasI GACNNNNNNGTC 1 cut(s) 1968
AatII GACGTC 1 cut(s) 3017
Acc16I TGCGCA 1 cut(s) 2276
Acc36I ACCTGC 3 cut(s) 418, 962, 1391
Acc65I GGTACC 1 cut(s) 942
AccB1I GGYRCC 3 cut(s) 942, 1507, 2256
AccI GTMKAC 2 cut(s) 801, 2815
AccII CGCG 1 cut(s) 11
AciI CCGC 9 cut(s) 11, 1007, 1396, 1867, 2207, 2251, 2784, 3141, 3189
AclWI GGATC 9 cut(s) 69, 81, 344, 412, 662, 706, 1192, 1545, 3206
AcoI YGGCCR 3 cut(s) 1864, 1984, 2461
AcsI RAATTY 9 cut(s) 318, 373, 765, 811, 1441, 1626, 2034, 2288, 3031
AcuI CTGAAG 7 cut(s) 93, 188, 885, 939, 2063, 2232, 2882
AcyI GRCGYC 1 cut(s) 3014
AdeI CACNNNGTG 1 cut(s) 3239
AfaI GTAC 7 cut(s) 591, 715, 944, 1205, 1410, 2519, 3317
AfiI CCNNNNNNNGG 4 cut(s) 565, 851, 1504, 1840
AflIII ACRYGT 2 cut(s) 2818, 2847
AjnI CCWGG 3 cut(s) 153, 1789, 3070
AjuI GAANNNNNNNTTGG 4 cut(s) 1613, 1645, 1922, 1954
AleI CACNNNNGTG 1 cut(s) 3023
AloI GAACNNNNNNTCC 2 cut(s) 2493, 2525
Alw21I GWGCWC 3 cut(s) 234, 2382, 3326
Alw26I GTCTC 4 cut(s) 639, 1092, 1092, 1795
AlwI GGATC 9 cut(s) 69, 81, 344, 412, 662, 706, 1192, 1545, 3206
AlwNI CAGNNNCTG 1 cut(s) 2210
AoxI GGCC 7 cut(s) 482, 567, 1864, 1984, 2461, 2853, 3121
ApaI GGGCCC 1 cut(s) 2857
ApeKI GCWGC 5 cut(s) 1328, 2204, 2579, 2732, 2735
ApoI RAATTY 9 cut(s) 318, 373, 765, 811, 1441, 1626, 2034, 2288, 3031
AseI ATTAAT 1 cut(s) 1449
Asp700I GAANNNNTTC 1 cut(s) 3154
Asp718I GGTACC 1 cut(s) 942
AspA2I CCTAGG 1 cut(s) 2918
AspLEI GCGC 3 cut(s) 11, 72, 2277
AspS9I GGNCC 7 cut(s) 483, 1249, 2853, 2854, 2915, 3174, 3290
AsuHPI GGTGA 8 cut(s) 149, 399, 530, 896, 995, 1310, 1415, 2526
AsuII TTCGAA 1 cut(s) 1543
AvaII GGWCC 4 cut(s) 1249, 2915, 3174, 3290
AvrII CCTAGG 1 cut(s) 2918
AxyI CCTNAGG 1 cut(s) 1512
BaeGI GKGCMC 2 cut(s) 2857, 3088
BalI TGGCCA 2 cut(s) 1986, 2463
BanI GGYRCC 3 cut(s) 942, 1507, 2256
BanII GRGCYC 5 cut(s) 857, 2077, 2382, 2857, 3326
BauI CACGAG 2 cut(s) 305, 1165
BbsI GAAGAC 2 cut(s) 48, 1478
Bbv12I GWGCWC 3 cut(s) 234, 2382, 3326
BbvI GCAGC 5 cut(s) 1315, 2216, 2566, 2744, 2747
BccI CCATC 6 cut(s) 578, 1571, 1851, 2063, 2986, 3281
BceAI ACGGC 1 cut(s) 61
BciT130I CCWGG 3 cut(s) 155, 1791, 3072
BciVI GTATCC 3 cut(s) 1652, 2320, 2559
BclI TGATCA 2 cut(s) 240, 925
BcoDI GTCTC 4 cut(s) 639, 1092, 1092, 1795
BfaI CTAG 8 cut(s) 114, 687, 1707, 2223, 2811, 2919, 3137, 3337
BfmI CTRYAG 2 cut(s) 2580, 3258
BfuAI ACCTGC 3 cut(s) 418, 962, 1391
BfuI GTATCC 3 cut(s) 1652, 2320, 2559
BglII AGATCT 1 cut(s) 2012
BisI GCNGC 8 cut(s) 1329, 1867, 2205, 2208, 2580, 2733, 2736, 2784
BlnI CCTAGG 1 cut(s) 2918
BlpI GCTNAGC 1 cut(s) 621
BlsI GCNGC 8 cut(s) 1330, 1868, 2206, 2209, 2581, 2734, 2737, 2785
Bme1390I CCNGG 3 cut(s) 155, 1791, 3072
Bme18I GGWCC 4 cut(s) 1249, 2915, 3174, 3290
BmgT120I GGNCC 7 cut(s) 483, 1249, 2853, 2854, 2915, 3174, 3290
BmiI GGNNCC 8 cut(s) 944, 1509, 2258, 2855, 3175, 3176, 3193, 3292
BmrFI CCNGG 3 cut(s) 155, 1791, 3072
BmsI GCATC 7 cut(s) 257, 493, 511, 1585, 2575, 2818, 2998
BpiI GAAGAC 2 cut(s) 48, 1478
BplI GAGNNNNNCTC 4 cut(s) 439, 471, 839, 871
BpmI CTGGAG 1 cut(s) 530
Bpu10I CCTNAGC 2 cut(s) 1911, 3056
Bpu1102I GCTNAGC 1 cut(s) 621
Bpu14I TTCGAA 1 cut(s) 1543
BpuEI CTTGAG 3 cut(s) 584, 1353, 2366
Bsa29I ATCGAT 1 cut(s) 3210
BsaHI GRCGYC 1 cut(s) 3014
BsaI GGTCTC 1 cut(s) 1092
BsaJI CCNNGG 7 cut(s) 390, 880, 948, 1499, 1789, 2918, 3102
Bsc4I CCNNNNNNNGG 4 cut(s) 565, 851, 1504, 1840
Bse1I ACTGG 6 cut(s) 485, 732, 940, 1063, 2677, 3151
Bse21I CCTNAGG 1 cut(s) 1512
Bse3DI GCAATG 5 cut(s) 269, 420, 1647, 2357, 2800
BseBI CCWGG 3 cut(s) 155, 1791, 3072
BseCI ATCGAT 1 cut(s) 3210
BseDI CCNNGG 7 cut(s) 390, 880, 948, 1499, 1789, 2918, 3102
BseLI CCNNNNNNNGG 4 cut(s) 565, 851, 1504, 1840
BseMI GCAATG 5 cut(s) 269, 420, 1647, 2357, 2800
BseMII CTCAG 8 cut(s) 612, 837, 1526, 1779, 1925, 1988, 2226, 2296
BseNI ACTGG 6 cut(s) 485, 732, 940, 1063, 2677, 3151
BseRI GAGGAG 6 cut(s) 1080, 1356, 1482, 2071, 2192, 2612
BseSI GKGCMC 2 cut(s) 2857, 3088
BseXI GCAGC 5 cut(s) 1315, 2216, 2566, 2744, 2747
BseYI CCCAGC 2 cut(s) 704, 3170
BsgI GTGCAG 1 cut(s) 708
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 7 cut(s) 484, 569, 1866, 1986, 2463, 2855, 3123
BshNI GGYRCC 3 cut(s) 942, 1507, 2256
BshVI ATCGAT 1 cut(s) 3210
BsiHKAI GWGCWC 3 cut(s) 234, 2382, 3326
BslFI GGGAC 2 cut(s) 721, 3187
BslI CCNNNNNNNGG 4 cut(s) 565, 851, 1504, 1840
BsmAI GTCTC 4 cut(s) 639, 1092, 1092, 1795
BsmBI CGTCTC 1 cut(s) 1092
BsmFI GGGAC 2 cut(s) 721, 3187
BsmI GAATGC 3 cut(s) 217, 1390, 2245
BsnI GGCC 7 cut(s) 484, 569, 1866, 1986, 2463, 2855, 3123
Bso31I GGTCTC 1 cut(s) 1092
Bsp119I TTCGAA 1 cut(s) 1543
Bsp120I GGGCCC 1 cut(s) 2853
Bsp1286I GDGCHC 7 cut(s) 234, 857, 2077, 2382, 2857, 3088, 3326
Bsp1407I TGTACA 2 cut(s) 2517, 3315
Bsp1720I GCTNAGC 1 cut(s) 621
Bsp19I CCATGG 1 cut(s) 1499
BspACI CCGC 9 cut(s) 11, 1007, 1396, 1867, 2207, 2251, 2784, 3141, 3189
BspANI GGCC 7 cut(s) 484, 569, 1866, 1986, 2463, 2855, 3123
BspCNI CTCAG 8 cut(s) 613, 838, 1525, 1778, 1924, 1987, 2227, 2295
BspDI ATCGAT 1 cut(s) 3210
BspFNI CGCG 1 cut(s) 11
BspHI TCATGA 2 cut(s) 1468, 2029
BspLI GGNNCC 8 cut(s) 944, 1509, 2258, 2855, 3175, 3176, 3193, 3292
BspMAI CTGCAG 1 cut(s) 2584
BspMI ACCTGC 3 cut(s) 418, 962, 1391
BspPI GGATC 9 cut(s) 69, 81, 344, 412, 662, 706, 1192, 1545, 3206
BspQI GCTCTTC 2 cut(s) 2082, 2370
BspT104I TTCGAA 1 cut(s) 1543
BspT107I GGYRCC 3 cut(s) 942, 1507, 2256
BspTNI GGTCTC 1 cut(s) 1092
BsrDI GCAATG 5 cut(s) 269, 420, 1647, 2357, 2800
BsrGI TGTACA 2 cut(s) 2517, 3315
BsrI ACTGG 6 cut(s) 485, 732, 940, 1063, 2677, 3151
BssECI CCNNGG 7 cut(s) 390, 880, 948, 1499, 1789, 2918, 3102
BssNI GRCGYC 1 cut(s) 3014
BssSI CACGAG 2 cut(s) 305, 1165
BssT1I CCWWGG 5 cut(s) 390, 880, 1499, 2918, 3102
Bst2BI CACGAG 2 cut(s) 305, 1165
Bst2UI CCWGG 3 cut(s) 155, 1791, 3072
Bst4CI ACNGT 2 cut(s) 1873, 3022
Bst6I CTCTTC 3 cut(s) 59, 2082, 2370
BstACI GRCGYC 1 cut(s) 3014
BstAPI GCANNNNNTGC 1 cut(s) 2359
BstAUI TGTACA 2 cut(s) 2517, 3315
BstBI TTCGAA 1 cut(s) 1543
BstC8I GCNNGC 6 cut(s) 1398, 1892, 2772, 2808, 2949, 3053
BstDSI CCRYGG 1 cut(s) 1499
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 3 cut(s) 11, 72, 2277
BstMAI GTCTC 4 cut(s) 639, 1092, 1092, 1795
BstMWI GCNNNNNNNGC 9 cut(s) 280, 530, 1370, 1759, 2201, 2350, 2359, 2729, 2780
BstNI CCWGG 3 cut(s) 155, 1791, 3072
BstNSI RCATGY 6 cut(s) 217, 620, 1910, 2143, 2822, 2851
BstSCI CCNGG 3 cut(s) 153, 1789, 3070
BstSFI CTRYAG 2 cut(s) 2580, 3258
BstSLI GKGCMC 2 cut(s) 2857, 3088
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 5 cut(s) 1315, 2216, 2566, 2744, 2747
BstV2I GAAGAC 2 cut(s) 48, 1478
BstX2I RGATCY 3 cut(s) 74, 1537, 2012
BstXI CCANNNNNNTGG 1 cut(s) 3185
BstYI RGATCY 3 cut(s) 74, 1537, 2012
Bsu15I ATCGAT 1 cut(s) 3210
Bsu36I CCTNAGG 1 cut(s) 1512
BsuI GTATCC 3 cut(s) 1652, 2320, 2559
BsuRI GGCC 7 cut(s) 484, 569, 1866, 1986, 2463, 2855, 3123
BsuTUI ATCGAT 1 cut(s) 3210
BtgI CCRYGG 1 cut(s) 1499
BtgZI GCGATG 1 cut(s) 2552
BtsI GCAGTG 3 cut(s) 1575, 2537, 3233
BtsIMutI CAGTG 8 cut(s) 478, 725, 1575, 1869, 2537, 2670, 3027, 3233
BveI ACCTGC 3 cut(s) 418, 962, 1391
Cac8I GCNNGC 6 cut(s) 1398, 1892, 2772, 2808, 2949, 3053
CaiI CAGNNNCTG 1 cut(s) 2210
CciI TCATGA 2 cut(s) 1468, 2029
CfoI GCGC 3 cut(s) 11, 72, 2277
Cfr13I GGNCC 7 cut(s) 483, 1249, 2853, 2854, 2915, 3174, 3290
ClaI ATCGAT 1 cut(s) 3210
CseI GACGC 3 cut(s) 1109, 2531, 3057
Csp6I GTAC 7 cut(s) 590, 714, 943, 1204, 1409, 2518, 3316
CspCI CAANNNNNGTGG 2 cut(s) 1227, 1262
CviQI GTAC 7 cut(s) 590, 714, 943, 1204, 1409, 2518, 3316
DraIII CACNNNGTG 1 cut(s) 3239
DrdI GACNNNNNNGTC 1 cut(s) 1968
DseDI GACNNNNNNGTC 1 cut(s) 1968
EaeI YGGCCR 3 cut(s) 1864, 1984, 2461
Eam1104I CTCTTC 3 cut(s) 59, 2082, 2370
EarI CTCTTC 3 cut(s) 59, 2082, 2370
EciI GGCGGA 2 cut(s) 3156, 3204
Ecl136II GAGCTC 2 cut(s) 2380, 3324
Eco130I CCWWGG 5 cut(s) 390, 880, 1499, 2918, 3102
Eco147I AGGCCT 1 cut(s) 569
Eco24I GRGCYC 5 cut(s) 857, 2077, 2382, 2857, 3326
Eco31I GGTCTC 1 cut(s) 1092
Eco47I GGWCC 4 cut(s) 1249, 2915, 3174, 3290
Eco53kI GAGCTC 2 cut(s) 2380, 3324
Eco57I CTGAAG 7 cut(s) 93, 188, 885, 939, 2063, 2232, 2882
Eco81I CCTNAGG 1 cut(s) 1512
EcoICRI GAGCTC 2 cut(s) 2380, 3324
EcoO109I RGGNCCY 3 cut(s) 2854, 2915, 3174
EcoRII CCWGG 3 cut(s) 153, 1789, 3070
EcoT14I CCWWGG 5 cut(s) 390, 880, 1499, 2918, 3102
EcoT22I ATGCAT 2 cut(s) 219, 2245
EcoT38I GRGCYC 5 cut(s) 857, 2077, 2382, 2857, 3326
ErhI CCWWGG 5 cut(s) 390, 880, 1499, 2918, 3102
Esp3I CGTCTC 1 cut(s) 1092
FaqI GGGAC 2 cut(s) 721, 3187
FauI CCCGC 1 cut(s) 1389
FauNDI CATATG 3 cut(s) 226, 930, 2131
FbaI TGATCA 2 cut(s) 240, 925
FblI GTMKAC 2 cut(s) 801, 2815
Fnu4HI GCNGC 8 cut(s) 1329, 1867, 2205, 2208, 2580, 2733, 2736, 2784
FriOI GRGCYC 5 cut(s) 857, 2077, 2382, 2857, 3326
Fsp4HI GCNGC 8 cut(s) 1329, 1867, 2205, 2208, 2580, 2733, 2736, 2784
FspBI CTAG 8 cut(s) 114, 687, 1707, 2223, 2811, 2919, 3137, 3337
FspI TGCGCA 1 cut(s) 2276
GlaI GCGC 3 cut(s) 10, 71, 2276
GluI GCNGC 8 cut(s) 1329, 1867, 2205, 2208, 2580, 2733, 2736, 2784
GsaI CCCAGC 2 cut(s) 708, 3174
GsuI CTGGAG 1 cut(s) 530
HaeIII GGCC 7 cut(s) 484, 569, 1866, 1986, 2463, 2855, 3123
HgaI GACGC 3 cut(s) 1109, 2531, 3057
HhaI GCGC 3 cut(s) 11, 72, 2277
Hin1I GRCGYC 1 cut(s) 3014
Hin6I GCGC 3 cut(s) 9, 70, 2275
HinP1I GCGC 3 cut(s) 9, 70, 2275
HincII GTYRAC 2 cut(s) 301, 1852
HindII GTYRAC 2 cut(s) 301, 1852
HindIII AAGCTT 1 cut(s) 2945
HphI GGTGA 8 cut(s) 149, 399, 530, 896, 995, 1310, 1415, 2526
Hpy166II GTNNAC 8 cut(s) 184, 301, 590, 802, 1852, 2520, 2816, 3018
Hpy8I GTNNAC 8 cut(s) 184, 301, 590, 802, 1852, 2520, 2816, 3018
Hpy99I CGWCG 5 cut(s) 1098, 2547, 2964, 3016, 3299
HpyCH4III ACNGT 2 cut(s) 1873, 3022
HpyCH4IV ACGT 3 cut(s) 2531, 2959, 3014
HpyF10VI GCNNNNNNNGC 9 cut(s) 280, 530, 1370, 1759, 2201, 2350, 2359, 2729, 2780
HpySE526I ACGT 3 cut(s) 2531, 2959, 3014
Hsp92I GRCGYC 1 cut(s) 3014
HspAI GCGC 3 cut(s) 9, 70, 2275
KflI GGGWCCC 1 cut(s) 3174
KpnI GGTACC 1 cut(s) 946
Ksp22I TGATCA 2 cut(s) 240, 925
LguI GCTCTTC 2 cut(s) 2082, 2370
LmnI GCTCC 3 cut(s) 1369, 2096, 3191
Lsp1109I GCAGC 5 cut(s) 1315, 2216, 2566, 2744, 2747
LweI GCATC 7 cut(s) 257, 493, 511, 1585, 2575, 2818, 2998
MaeI CTAG 8 cut(s) 114, 687, 1707, 2223, 2811, 2919, 3137, 3337
MaeII ACGT 3 cut(s) 2531, 2959, 3014
MaeIII GTNAC 4 cut(s) 872, 1728, 2492, 2532
MfeI CAATTG 1 cut(s) 1605
MflI RGATCY 3 cut(s) 74, 1537, 2012
MhlI GDGCHC 7 cut(s) 234, 857, 2077, 2382, 2857, 3088, 3326
MlsI TGGCCA 2 cut(s) 1986, 2463
MluNI TGGCCA 2 cut(s) 1986, 2463
MlyI GAGTC 5 cut(s) 457, 552, 1313, 2153, 2384
MmeI TCCRAC 3 cut(s) 589, 2597, 3256
Mox20I TGGCCA 2 cut(s) 1986, 2463
Mph1103I ATGCAT 2 cut(s) 219, 2245
MroXI GAANNNNTTC 1 cut(s) 3154
MscI TGGCCA 2 cut(s) 1986, 2463
MslI CAYNNNNRTG 4 cut(s) 288, 309, 1211, 3023
Msp20I TGGCCA 2 cut(s) 1986, 2463
MspA1I CMGCKG 2 cut(s) 704, 2207
MspR9I CCNGG 3 cut(s) 155, 1791, 3072
MunI CAATTG 1 cut(s) 1605
Mva1269I GAATGC 3 cut(s) 217, 1390, 2245
MvaI CCWGG 3 cut(s) 155, 1791, 3072
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 9 cut(s) 280, 530, 1370, 1759, 2201, 2350, 2359, 2729, 2780
NcoI CCATGG 1 cut(s) 1499
NdeI CATATG 3 cut(s) 226, 930, 2131
NlaIV GGNNCC 8 cut(s) 944, 1509, 2258, 2855, 3175, 3176, 3193, 3292
NmeAIII GCCGAG 1 cut(s) 929
NmuCI GTSAC 3 cut(s) 1728, 2492, 2532
NsbI TGCGCA 1 cut(s) 2276
NsiI ATGCAT 2 cut(s) 219, 2245
NspI RCATGY 6 cut(s) 217, 620, 1910, 2143, 2822, 2851
NspV TTCGAA 1 cut(s) 1543
OliI CACNNNNGTG 1 cut(s) 3023
PagI TCATGA 2 cut(s) 1468, 2029
PaqCI CACCTGC 2 cut(s) 418, 1391
PceI AGGCCT 1 cut(s) 569
PciI ACATGT 2 cut(s) 2818, 2847
PciSI GCTCTTC 2 cut(s) 2082, 2370
PctI GAATGC 3 cut(s) 217, 1390, 2245
PdmI GAANNNNTTC 1 cut(s) 3154
PfeI GAWTC 6 cut(s) 887, 959, 1271, 1472, 2367, 2702
PflFI GACNNNGTC 1 cut(s) 1965
PkrI GCNGC 8 cut(s) 1330, 1868, 2206, 2209, 2581, 2734, 2737, 2785
PleI GAGTC 5 cut(s) 456, 551, 1312, 2153, 2384
PpsI GAGTC 5 cut(s) 456, 551, 1312, 2153, 2384
PpuMI RGGWCCY 2 cut(s) 2915, 3174
PscI ACATGT 2 cut(s) 2818, 2847
PshBI ATTAAT 1 cut(s) 1449
Psp124BI GAGCTC 2 cut(s) 2382, 3326
Psp5II RGGWCCY 2 cut(s) 2915, 3174
Psp6I CCWGG 3 cut(s) 153, 1789, 3070
PspFI CCCAGC 2 cut(s) 704, 3170
PspGI CCWGG 3 cut(s) 153, 1789, 3070
PspN4I GGNNCC 8 cut(s) 944, 1509, 2258, 2855, 3175, 3176, 3193, 3292
PspOMI GGGCCC 1 cut(s) 2853
PspPI GGNCC 7 cut(s) 483, 1249, 2853, 2854, 2915, 3174, 3290
PspPPI RGGWCCY 2 cut(s) 2915, 3174
PstI CTGCAG 1 cut(s) 2584
PstNI CAGNNNCTG 1 cut(s) 2210
PsuI RGATCY 3 cut(s) 74, 1537, 2012
PsyI GACNNNGTC 1 cut(s) 1965
PvuII CAGCTG 1 cut(s) 704
RsaI GTAC 7 cut(s) 591, 715, 944, 1205, 1410, 2519, 3317
RsaNI GTAC 7 cut(s) 590, 714, 943, 1204, 1409, 2518, 3316
RseI CAYNNNNRTG 4 cut(s) 288, 309, 1211, 3023
SacI GAGCTC 2 cut(s) 2382, 3326
SapI GCTCTTC 2 cut(s) 2082, 2370
SatI GCNGC 8 cut(s) 1329, 1867, 2205, 2208, 2580, 2733, 2736, 2784
Sau96I GGNCC 7 cut(s) 483, 1249, 2853, 2854, 2915, 3174, 3290
SchI GAGTC 5 cut(s) 457, 552, 1313, 2153, 2384
ScrFI CCNGG 3 cut(s) 155, 1791, 3072
SduI GDGCHC 7 cut(s) 234, 857, 2077, 2382, 2857, 3088, 3326
SfaNI GCATC 7 cut(s) 257, 493, 511, 1585, 2575, 2818, 2998
SfcI CTRYAG 2 cut(s) 2580, 3258
SfuI TTCGAA 1 cut(s) 1543
SinI GGWCC 4 cut(s) 1249, 2915, 3174, 3290
SmiMI CAYNNNNRTG 4 cut(s) 288, 309, 1211, 3023
SmlI CTYRAG 3 cut(s) 563, 1368, 2381
SmoI CTYRAG 3 cut(s) 563, 1368, 2381
SseBI AGGCCT 1 cut(s) 569
SsiI CCGC 9 cut(s) 11, 1007, 1396, 1867, 2207, 2251, 2784, 3141, 3189
SspI AATATT 3 cut(s) 580, 2407, 2625
SspMI CTAG 8 cut(s) 114, 687, 1707, 2223, 2811, 2919, 3137, 3337
SstI GAGCTC 2 cut(s) 2382, 3326
StuI AGGCCT 1 cut(s) 569
StyD4I CCNGG 3 cut(s) 153, 1789, 3070
StyI CCWWGG 5 cut(s) 390, 880, 1499, 2918, 3102
TaaI ACNGT 2 cut(s) 1873, 3022
TaiI ACGT 3 cut(s) 2534, 2962, 3017
TaqII GACCGA 1 cut(s) 1869
TatI WGTACW 4 cut(s) 1203, 1408, 2517, 3315
TauI GCSGC 3 cut(s) 1869, 2210, 2786
TfiI GAWTC 6 cut(s) 887, 959, 1271, 1472, 2367, 2702
TscAI CASTG 8 cut(s) 485, 732, 1582, 1876, 2544, 2677, 3027, 3240
TseFI GTSAC 3 cut(s) 1728, 2492, 2532
TseI GCWGC 5 cut(s) 1328, 2204, 2579, 2732, 2735
Tsp45I GTSAC 3 cut(s) 1728, 2492, 2532
TspGWI ACGGA 2 cut(s) 249, 611
TspRI CASTG 8 cut(s) 485, 732, 1582, 1876, 2544, 2677, 3027, 3240
Tth111I GACNNNGTC 1 cut(s) 1965
VpaK11BI GGWCC 4 cut(s) 1249, 2915, 3174, 3290
VspI ATTAAT 1 cut(s) 1449
XapI RAATTY 9 cut(s) 318, 373, 765, 811, 1441, 1626, 2034, 2288, 3031
XbaI TCTAGA 2 cut(s) 1706, 2222
XceI RCATGY 6 cut(s) 217, 620, 1910, 2143, 2822, 2851
XcmI CCANNNNNNNNNTGG 1 cut(s) 724
XmaJI CCTAGG 1 cut(s) 2918
XmiI GTMKAC 2 cut(s) 801, 2815
XmnI GAANNNNTTC 1 cut(s) 3154
XspI CTAG 8 cut(s) 114, 687, 1707, 2223, 2811, 2919, 3137, 3337
ZraI GACGTC 1 cut(s) 3015
Zsp2I ATGCAT 2 cut(s) 219, 2245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.