MD08G1168200.v1.1

3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0007261
Physical Location & Seq
Reverse (-)
19973851 .. 19979689
5839 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1168200.v1.1.491

Sequence Viewer

Length: 3318 bp
ATGAAAGACTCGGTTGTTCAAGCGGTGGTTAAAAGGGTTGAAGACGGCGATAAGGAGAAGAAGAAGAAGCGCCGACCCAATCGCCGATCCAGACAAAATAATTCCACTTCAGCTAGTCCGAGAACTGAAGTATCCGAGTGCCTGGCAAATGGAAGAATTTCTAATCATGTAACCACATCCTTGAAGCAACCTCAGTTAGACATGCATCCTCCATATGAGCACGGAATGATAAAAGCATCTAATTTGGCCTTCAATTCATTGCCAACGATGCACATCAATGAACAAGTCAATCACGAGGATGTGCAAATTTTGGTAAATCAACATTCTCTCCCATGTGATCCTGCTGGAAGATTTATTTCAAATTCTTGCCCTGAACCTGTGGCTTGTGGAGGATCGCCTGGAATGTTCAAGGATTTCCCTCCTCATCATGCTGAGAGTTGTGCTCGAAGAAAATGTTTTACTTCACACTGGCCCATGGAGGCTGTTAATGACGCTCTAGAGAAAGGTGATGCTTTTAAAGCTTTGTTTCGAGTCAATGCACACAACCGATTGGAGGCCTATTGCAAAATTGATGGTGTACCAACAGACATTCTCATTGATGGACTTGCTGAGCAGAATAGAGCTATGGAAGGAGACATTGTGGCGATTAAGGTTGATCCTTTGGCGTTATGGATGAGGATGAAAGGATCAGCTGGGACTTGTACCAGTTCTGCACTGGTGGAGGATTTTAACTTACCACAACAAGCTAATGAAATTTCTGGTCTCAACTGCAAAGGTAAGGCTAAGGTAGACGAGGTGGATTTGTATGCTAATGGTAGAAGAAGCCTGCTTCCTGAGAGGAGGTCATGTCCTCAGGAAAGTACTTATCCTGGTGAATCTATTACTTCAAAGCCAATTGGACAAACAAGTTATGATCATGTTGCTGGGAGGTACCCCTCGGCTTCAGATTTTTTACAAGAAGGTTCTCATGGTGAGCAGAATGAAGTTGCAGTTGCAATAGAGAGGATATGTGCCATGATCAGTTCATTCCCTTCAAAACGACCAACTGGCAGGGTCGTTGCTATCATTGAAAGGTCTCCACGTCGAGACTCTGTTGTTGGTTTCCTTCATGTTAAGAAGTGGATTGCTTACAGGGAAGTTTGCAGAAACGATATGGGAAAGAACAAAAATGCATCATTTTCTAGTGATGAGTACATTCAGATGATCCCAACTGACCCAAGATTTCCGAAAATGGTGGTCCTTGTGAGAACCCTGCCTGATTCAATCAAGAAGAGATTGGAGAATGGTGATGAGTCAATTGAAATGGAGTTGTTTGCTGCCCGAATAGATGAATGGGATGAGGAAAGTTCTGCTCCCCAAGCTGTCATCTTGAATGCTTTTGGCCGGGCAAGCGAAGTACAGCCACAAATTGAAGCAATTTTATTCCAAAATTCAATTAATTCATCAGATTTTTCTCATGAATCACTTTCCTGTCTTCCTCATCTCACGTGGGAGGTGCCTCAGGAGGAAATCAAAAGTAGAAAGGATCTTCGAAACCTGTGCATATTAACCATTGACCCTTCAACTGCTACTGATCTGGATGATGCACTCTCAGTTGAGAAATTATCCAACGGAATTTTCAGAGTTGGTATCCACATTGCTGATGTGTCGTATTTTGTTTTGCCCGATACACCGTTAGACGAAGAAGCTCAATCTAGATCAACAAGTGTGTATATGTCACAGCGGAAATTACCAATGTTGCCTCCTTTGCTCTCAGAGAATATTGGTTCGCTTAACCCAGGAGTAGAGAGACTAGCATTTTCAATTTTCTTGGACATCAACTATGTTGGGGATGTTGTTGACCGATGGATTGGCCGCACTGTGATAAGATCGTGTTGCAAACTTTCTTATGAACATGCTCAGGAGATTATCAATGGGAAACTTAATTTGGAAAGTTCTAACATTTTAGGAAAGGGTTTTCCTCAGTTGCATGGCCATTTTGAATGGTCTGATGTAATTAGATCTGTTAAAGATCTTCTTGAAATTTCCAGAGTTTTGAAGGAGAGGAGGTTTAGTGATGGGGCTCTTCAGCTTGAGAGCTCTAAAATCGTTATTCTGTTTGACGAATATGGAGTTCCATATGATAGCATGTATTCTGAAAGGAAGGACTCAAATTTTCTTGTCGAGGAGTTTATGCTTTTGGCAAATACAACAGCAGCTGAAGTCATATCTAGAGCTTTTCCTGAGAGTGCATTATTGCGGAGGCACCCAGAACCTAATATGCGCAAACTCAGGGAATTTGAAGCATTTTGTTCTAAGCATGGTTTGGAATTGGATACGTCGTCTTCTGGGCTGTTCCAGCAATCATTGCTGAGAATCAGGGAAGAGCTCAAGGATGATTCTGTTCTATTCAATATTCTTATGAGTTATGCTACAAAACCAATGCAGTTGGCTTCTTATTTTTGCAGCGGCGAATTAAAAGACAGAGAAAATGATTGGGGTCACTATGGACTTGCTGTTCCTTTGTACACTCATTTTACGTCTCCACTGCGTCGGTATCCAGATATTGTGGTGCATCGCACGCTGGCTGCAGCCGTAGAGGCAGAGGAGTTATTTTTGAAACATCGGAGAATGTTGAATAATTTTAGTAGGGGGGATGAAGTTAAAATGAGATGTTTTACTGGTATTTATTTTGATAAGAATGTTGCTGAATCCTGTGAAATCAAGGAAGCACTATCGGCTGCAGCAATAAAGCATAGAGTTCCCTGCTCTGAAATGCTTGCTGATGTTGCTGCTTATTGCAATGCAAGAAAGCTGGCTAGTAGACATGTCAAGGATGCTTGTGATAAGCTCTACATGTGGGCCCTGCTGAAGAAAAAGGAGATTGTATTATCAGAAGCAAGAGTATTGGGTGTGGGGCCTAGATTCATGTCCATTTATATTCACAAGCTTGCTGTTGAACGTCGAATATATTATGATGAAGTTGAAGGCTTGATGGTAGAATGGCTTGATGCTACATCCACATTGGTGCTGAATTTATGTTCTAACAGACGCTCGCACAGGAGAGGTAGTCCTGGTAAGTGGAGGGCGCTTGAAGATGTTGCCTTGGTTGTAAGACCTTATGACCTCAAAGCTGAACTAGGTGGAACTGGTAATAGTTCTAAAGAGGGTGCCGTGGTCCAAGATGTTGGCGTAGCCACCCATAGCGAAATTGATGCAGTCGTTTTCCCCCTTACACTGCGTGTTCTTTCAACATTTCCTGTAGTGCTTCATGCAATCGGGGGGGATTATGGACCCGTTGATATTGGGGCAAGGCTGTACGTGAGCTCATATCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000175 GO:0000178 GO:0000278 GO:0000280 GO:0000287 GO:0000291 GO:0000819 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005844 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0008285 GO:0008408 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010586 GO:0010587 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019222 GO:0019439 GO:0019827 GO:0022402 GO:0022613 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035770 GO:0036464 GO:0042127 GO:0042254 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0046872 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051276 GO:0051304 GO:0051306 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0097159 GO:0098727 GO:0098813 GO:0140014 GO:0140098 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1902494 GO:1903047 GO:1905354 GO:1990074 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNB

Protein Analysis

1106

Amino Acids

123.98

Weight (kDa)

6.27

Isoelectric Point (pI)

54.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Dis3 PF17849 398 - 477 2.1e-15 Dis3-like cold-shock domain 2 (CSD2)
RNB PF00773 507 - 859 9.6e-100 RNB domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2264
Acc65I GGTACC 1 cut(s) 930
AccB1I GGYRCC 4 cut(s) 930, 1495, 2244, 3149
AccI GTMKAC 2 cut(s) 789, 2803
AciI CCGC 5 cut(s) 23, 1723, 1855, 2239, 2448
AclWI GGATC 7 cut(s) 81, 332, 400, 650, 694, 1198, 1533
AcoI YGGCCR 3 cut(s) 1381, 1852, 1972
AcuI CTGAAG 6 cut(s) 93, 147, 927, 2051, 2220, 2870
AcvI CACGTG 1 cut(s) 1488
AdeI CACNNNGTG 1 cut(s) 3221
AfaI GTAC 8 cut(s) 579, 703, 862, 932, 1193, 1398, 2507, 3299
AfiI CCNNNNNNNGG 1 cut(s) 553
AflIII ACRYGT 2 cut(s) 2806, 2835
AjiI CACGTC 1 cut(s) 1082
AjnI CCWGG 5 cut(s) 141, 397, 868, 1777, 3052
AjuI GAANNNNNNNTTGG 4 cut(s) 1601, 1633, 1910, 1942
AleI CACNNNNGTG 1 cut(s) 3005
AloI GAACNNNNNNTCC 2 cut(s) 2481, 2513
Alw21I GWGCWC 5 cut(s) 222, 445, 2081, 2370, 3308
Alw26I GTCTC 6 cut(s) 627, 767, 1080, 1080, 1783, 2526
AlwI GGATC 7 cut(s) 81, 332, 400, 650, 694, 1198, 1533
AlwNI CAGNNNCTG 1 cut(s) 2198
AoxI GGCC 8 cut(s) 246, 470, 555, 1381, 1852, 1972, 2841, 2897
ApaI GGGCCC 1 cut(s) 2845
ApeKI GCWGC 8 cut(s) 1316, 2195, 2445, 2567, 2570, 2720, 2723, 2771
AseI ATTAAT 1 cut(s) 1437
Asp700I GAANNNNTTC 1 cut(s) 157
Asp718I GGTACC 1 cut(s) 930
AspLEI GCGC 3 cut(s) 72, 2265, 3070
AspS9I GGNCC 7 cut(s) 471, 1237, 2841, 2842, 2897, 3157, 3272
AsuC2I CCSGG 1 cut(s) 1385
AsuHPI GGTGA 4 cut(s) 518, 884, 983, 1298
AsuII TTCGAA 1 cut(s) 1531
AvaII GGWCC 3 cut(s) 1237, 3157, 3272
AxyI CCTNAGG 2 cut(s) 852, 1500
BaeGI GKGCMC 1 cut(s) 2845
BaeI ACNNNNGTAYC 2 cut(s) 114, 147
BalI TGGCCA 1 cut(s) 1974
BanI GGYRCC 4 cut(s) 930, 1495, 2244, 3149
BanII GRGCYC 5 cut(s) 2065, 2081, 2370, 2845, 3308
BauI CACGAG 1 cut(s) 293
BbrPI CACGTG 1 cut(s) 1488
BbsI GAAGAC 3 cut(s) 48, 1466, 2316
Bbv12I GWGCWC 5 cut(s) 222, 445, 2081, 2370, 3308
BbvI GCAGC 8 cut(s) 1303, 2207, 2457, 2554, 2582, 2707, 2735, 2758
BccI CCATC 5 cut(s) 566, 593, 1839, 2051, 2968
BceAI ACGGC 3 cut(s) 61, 2558, 3137
BcgI CGANNNNNNTGC 4 cut(s) 1521, 1555, 3176, 3210
BciT130I CCWGG 5 cut(s) 143, 399, 870, 1779, 3054
BciVI GTATCC 4 cut(s) 142, 1640, 2308, 2547
BclI TGATCA 2 cut(s) 913, 1017
BcnI CCSGG 1 cut(s) 1385
BcoDI GTCTC 6 cut(s) 627, 767, 1080, 1080, 1783, 2526
BfaI CTAG 9 cut(s) 114, 497, 1182, 1695, 1793, 2211, 2799, 2901, 3119
BfmI CTRYAG 3 cut(s) 2568, 2721, 3240
BfoI RGCGCY 2 cut(s) 73, 3071
BfuI GTATCC 4 cut(s) 142, 1640, 2308, 2547
BglI GCCNNNNNGGC 1 cut(s) 2579
BglII AGATCT 2 cut(s) 2000, 2011
BlpI GCTNAGC 1 cut(s) 609
BmcAI AGTACT 1 cut(s) 862
Bme1390I CCNGG 6 cut(s) 143, 399, 870, 1385, 1779, 3054
Bme18I GGWCC 3 cut(s) 1237, 3157, 3272
BmgBI CACGTC 1 cut(s) 1082
BmgT120I GGNCC 7 cut(s) 471, 1237, 2841, 2842, 2897, 3157, 3272
BmiI GGNNCC 7 cut(s) 932, 1497, 2246, 2843, 2898, 3151, 3274
BmrFI CCNGG 6 cut(s) 143, 399, 870, 1385, 1779, 3054
BpiI GAAGAC 3 cut(s) 48, 1466, 2316
BplI GAGNNNNNCTC 2 cut(s) 427, 459
Bpu10I CCTNAGC 2 cut(s) 783, 1899
Bpu1102I GCTNAGC 1 cut(s) 609
Bpu14I TTCGAA 1 cut(s) 1531
BpuEI CTTGAG 2 cut(s) 2093, 2354
BpuMI CCSGG 1 cut(s) 1385
BsaAI YACGTR 2 cut(s) 1488, 3301
BsaBI GATNNNNATC 1 cut(s) 272
BsaI GGTCTC 2 cut(s) 767, 1080
BsaJI CCNNGG 5 cut(s) 474, 936, 1777, 3084, 3153
Bsc4I CCNNNNNNNGG 1 cut(s) 553
Bse1I ACTGG 6 cut(s) 473, 705, 720, 1051, 2665, 3133
Bse21I CCTNAGG 2 cut(s) 852, 1500
Bse3DI GCAATG 4 cut(s) 257, 1635, 2345, 2788
Bse8I GATNNNNATC 1 cut(s) 272
BseBI CCWGG 5 cut(s) 143, 399, 870, 1779, 3054
BseDI CCNNGG 5 cut(s) 474, 936, 1777, 3084, 3153
BseJI GATNNNNATC 1 cut(s) 272
BseLI CCNNNNNNNGG 1 cut(s) 553
BseMI GCAATG 4 cut(s) 257, 1635, 2345, 2788
BseNI ACTGG 6 cut(s) 473, 705, 720, 1051, 2665, 3133
BseRI GAGGAG 5 cut(s) 411, 853, 2059, 2180, 2600
BseSI GKGCMC 1 cut(s) 2845
BseXI GCAGC 8 cut(s) 1303, 2207, 2457, 2554, 2582, 2707, 2735, 2758
BseYI CCCAGC 2 cut(s) 692, 923
BsgI GTGCAG 1 cut(s) 696
BshFI GGCC 8 cut(s) 248, 472, 557, 1383, 1854, 1974, 2843, 2899
BshNI GGYRCC 4 cut(s) 930, 1495, 2244, 3149
BsiHKAI GWGCWC 5 cut(s) 222, 445, 2081, 2370, 3308
BsiSI CCGG 1 cut(s) 1384
BslFI GGGAC 1 cut(s) 709
BslI CCNNNNNNNGG 1 cut(s) 553
BsmAI GTCTC 6 cut(s) 627, 767, 1080, 1080, 1783, 2526
BsmBI CGTCTC 1 cut(s) 2526
BsmFI GGGAC 1 cut(s) 709
BsmI GAATGC 1 cut(s) 1378
BsnI GGCC 8 cut(s) 248, 472, 557, 1383, 1854, 1974, 2843, 2899
Bso31I GGTCTC 2 cut(s) 767, 1080
Bsp119I TTCGAA 1 cut(s) 1531
Bsp120I GGGCCC 1 cut(s) 2841
Bsp1286I GDGCHC 7 cut(s) 222, 445, 2065, 2081, 2370, 2845, 3308
Bsp1407I TGTACA 1 cut(s) 2505
Bsp1720I GCTNAGC 1 cut(s) 609
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 5 cut(s) 23, 1723, 1855, 2239, 2448
BspANI GGCC 8 cut(s) 248, 472, 557, 1383, 1854, 1974, 2843, 2899
BspHI TCATGA 1 cut(s) 1456
BspLI GGNNCC 7 cut(s) 932, 1497, 2246, 2843, 2898, 3151, 3274
BspMAI CTGCAG 2 cut(s) 2572, 2725
BspPI GGATC 7 cut(s) 81, 332, 400, 650, 694, 1198, 1533
BspQI GCTCTTC 2 cut(s) 2070, 2358
BspT104I TTCGAA 1 cut(s) 1531
BspT107I GGYRCC 4 cut(s) 930, 1495, 2244, 3149
BspTNI GGTCTC 2 cut(s) 767, 1080
BsrDI GCAATG 4 cut(s) 257, 1635, 2345, 2788
BsrGI TGTACA 1 cut(s) 2505
BsrI ACTGG 6 cut(s) 473, 705, 720, 1051, 2665, 3133
BssECI CCNNGG 5 cut(s) 474, 936, 1777, 3084, 3153
BssSI CACGAG 1 cut(s) 293
BssT1I CCWWGG 2 cut(s) 474, 3084
Bst2BI CACGAG 1 cut(s) 293
Bst2UI CCWGG 5 cut(s) 143, 399, 870, 1779, 3054
Bst4CI ACNGT 2 cut(s) 1674, 1861
Bst6I CTCTTC 3 cut(s) 1265, 2070, 2358
BstAPI GCANNNNNTGC 1 cut(s) 2347
BstAUI TGTACA 1 cut(s) 2505
BstBAI YACGTR 2 cut(s) 1488, 3301
BstBI TTCGAA 1 cut(s) 1531
BstC8I GCNNGC 8 cut(s) 827, 1390, 2561, 2565, 2760, 2796, 2931, 3035
BstDSI CCRYGG 2 cut(s) 474, 3153
BstH2I RGCGCY 2 cut(s) 73, 3071
BstHHI GCGC 3 cut(s) 72, 2265, 3070
BstMAI GTCTC 6 cut(s) 627, 767, 1080, 1080, 1783, 2526
BstNI CCWGG 5 cut(s) 143, 399, 870, 1779, 3054
BstNSI RCATGY 5 cut(s) 205, 1898, 2131, 2810, 2839
BstSCI CCNGG 6 cut(s) 141, 397, 868, 1383, 1777, 3052
BstSFI CTRYAG 3 cut(s) 2568, 2721, 3240
BstSLI GKGCMC 1 cut(s) 2845
BstV1I GCAGC 8 cut(s) 1303, 2207, 2457, 2554, 2582, 2707, 2735, 2758
BstV2I GAAGAC 3 cut(s) 48, 1466, 2316
BstX2I RGATCY 3 cut(s) 1525, 2000, 2011
BstXI CCANNNNNNTGG 1 cut(s) 3167
BstYI RGATCY 3 cut(s) 1525, 2000, 2011
Bsu36I CCTNAGG 2 cut(s) 852, 1500
BsuI GTATCC 4 cut(s) 142, 1640, 2308, 2547
BsuRI GGCC 8 cut(s) 248, 472, 557, 1383, 1854, 1974, 2843, 2899
BtgI CCRYGG 2 cut(s) 474, 3153
BtgZI GCGATG 1 cut(s) 2540
BtrI CACGTC 1 cut(s) 1082
BtsI GCAGTG 2 cut(s) 2525, 3215
BtsIMutI CAGTG 5 cut(s) 466, 713, 1857, 2525, 3215
Cac8I GCNNGC 8 cut(s) 827, 1390, 2561, 2565, 2760, 2796, 2931, 3035
CaiI CAGNNNCTG 1 cut(s) 2198
CciI TCATGA 1 cut(s) 1456
CfoI GCGC 3 cut(s) 72, 2265, 3070
Cfr13I GGNCC 7 cut(s) 471, 1237, 2841, 2842, 2897, 3157, 3272
CseI GACGC 3 cut(s) 500, 2519, 3039
Csp6I GTAC 8 cut(s) 578, 702, 861, 931, 1192, 1397, 2506, 3298
CviQI GTAC 8 cut(s) 578, 702, 861, 931, 1192, 1397, 2506, 3298
DraI TTTAAA 1 cut(s) 517
DraIII CACNNNGTG 1 cut(s) 3221
EaeI YGGCCR 3 cut(s) 1381, 1852, 1972
Eam1104I CTCTTC 3 cut(s) 1265, 2070, 2358
EarI CTCTTC 3 cut(s) 1265, 2070, 2358
Ecl136II GAGCTC 3 cut(s) 2079, 2368, 3306
Eco130I CCWWGG 2 cut(s) 474, 3084
Eco147I AGGCCT 1 cut(s) 557
Eco24I GRGCYC 5 cut(s) 2065, 2081, 2370, 2845, 3308
Eco31I GGTCTC 2 cut(s) 767, 1080
Eco47I GGWCC 3 cut(s) 1237, 3157, 3272
Eco53kI GAGCTC 3 cut(s) 2079, 2368, 3306
Eco57I CTGAAG 6 cut(s) 93, 147, 927, 2051, 2220, 2870
Eco72I CACGTG 1 cut(s) 1488
Eco81I CCTNAGG 2 cut(s) 852, 1500
EcoICRI GAGCTC 3 cut(s) 2079, 2368, 3306
EcoO109I RGGNCCY 2 cut(s) 2842, 2897
EcoRII CCWGG 5 cut(s) 141, 397, 868, 1777, 3052
EcoT14I CCWWGG 2 cut(s) 474, 3084
EcoT22I ATGCAT 2 cut(s) 207, 1174
EcoT38I GRGCYC 5 cut(s) 2065, 2081, 2370, 2845, 3308
ErhI CCWWGG 2 cut(s) 474, 3084
Esp3I CGTCTC 1 cut(s) 2526
FalI AAGNNNNNCTT 2 cut(s) 2001, 2033
FaqI GGGAC 1 cut(s) 709
FauNDI CATATG 2 cut(s) 214, 2119
FbaI TGATCA 2 cut(s) 913, 1017
FblI GTMKAC 2 cut(s) 789, 2803
FriOI GRGCYC 5 cut(s) 2065, 2081, 2370, 2845, 3308
FspBI CTAG 9 cut(s) 114, 497, 1182, 1695, 1793, 2211, 2799, 2901, 3119
FspI TGCGCA 1 cut(s) 2264
GlaI GCGC 3 cut(s) 71, 2264, 3069
GsaI CCCAGC 2 cut(s) 696, 927
HaeII RGCGCY 2 cut(s) 73, 3071
HaeIII GGCC 8 cut(s) 248, 472, 557, 1383, 1854, 1974, 2843, 2899
HapII CCGG 1 cut(s) 1384
HgaI GACGC 3 cut(s) 500, 2519, 3039
HhaI GCGC 3 cut(s) 72, 2265, 3070
Hin6I GCGC 3 cut(s) 70, 2263, 3068
HinP1I GCGC 3 cut(s) 70, 2263, 3068
HincII GTYRAC 1 cut(s) 1840
HindII GTYRAC 1 cut(s) 1840
HindIII AAGCTT 2 cut(s) 519, 2927
HpaII CCGG 1 cut(s) 1384
HphI GGTGA 4 cut(s) 518, 884, 983, 1298
Hpy166II GTNNAC 5 cut(s) 578, 790, 1840, 2508, 2804
Hpy8I GTNNAC 5 cut(s) 578, 790, 1840, 2508, 2804
Hpy99I CGWCG 4 cut(s) 1086, 2323, 2535, 2946
HpyAV CCTTC 9 cut(s) 259, 623, 953, 1041, 1115, 1569, 2032, 2137, 2960
HpyCH4III ACNGT 2 cut(s) 1674, 1861
HpyCH4IV ACGT 6 cut(s) 1081, 1487, 2318, 2519, 2941, 3300
HpySE526I ACGT 6 cut(s) 1081, 1487, 2318, 2519, 2941, 3300
HspAI GCGC 3 cut(s) 70, 2263, 3068
KpnI GGTACC 1 cut(s) 934
Ksp22I TGATCA 2 cut(s) 913, 1017
LguI GCTCTTC 2 cut(s) 2070, 2358
LmnI GCTCC 1 cut(s) 1357
Lsp1109I GCAGC 8 cut(s) 1303, 2207, 2457, 2554, 2582, 2707, 2735, 2758
MaeI CTAG 9 cut(s) 114, 497, 1182, 1695, 1793, 2211, 2799, 2901, 3119
MaeII ACGT 6 cut(s) 1081, 1487, 2318, 2519, 2941, 3300
MaeIII GTNAC 3 cut(s) 169, 1716, 2480
MfeI CAATTG 2 cut(s) 894, 1296
MflI RGATCY 3 cut(s) 1525, 2000, 2011
MhlI GDGCHC 7 cut(s) 222, 445, 2065, 2081, 2370, 2845, 3308
MlsI TGGCCA 1 cut(s) 1974
MluNI TGGCCA 1 cut(s) 1974
MlyI GAGTC 5 cut(s) 2, 540, 1082, 1301, 2141
MmeI TCCRAC 1 cut(s) 1632
Mox20I TGGCCA 1 cut(s) 1974
Mph1103I ATGCAT 2 cut(s) 207, 1174
MroXI GAANNNNTTC 1 cut(s) 157
MscI TGGCCA 1 cut(s) 1974
MslI CAYNNNNRTG 4 cut(s) 276, 297, 1199, 3005
Msp20I TGGCCA 1 cut(s) 1974
MspA1I CMGCKG 4 cut(s) 692, 1723, 2198, 2448
MspI CCGG 1 cut(s) 1384
MspR9I CCNGG 6 cut(s) 143, 399, 870, 1385, 1779, 3054
MunI CAATTG 2 cut(s) 894, 1296
Mva1269I GAATGC 1 cut(s) 1378
MvaI CCWGG 5 cut(s) 143, 399, 870, 1779, 3054
NciI CCSGG 1 cut(s) 1385
NcoI CCATGG 1 cut(s) 474
NdeI CATATG 2 cut(s) 214, 2119
NlaIV GGNNCC 7 cut(s) 932, 1497, 2246, 2843, 2898, 3151, 3274
NmeAIII GCCGAG 1 cut(s) 917
NmuCI GTSAC 2 cut(s) 1716, 2480
NsbI TGCGCA 1 cut(s) 2264
NsiI ATGCAT 2 cut(s) 207, 1174
NspI RCATGY 5 cut(s) 205, 1898, 2131, 2810, 2839
NspV TTCGAA 1 cut(s) 1531
OliI CACNNNNGTG 1 cut(s) 3005
PagI TCATGA 1 cut(s) 1456
PceI AGGCCT 1 cut(s) 557
PciI ACATGT 2 cut(s) 2806, 2835
PciSI GCTCTTC 2 cut(s) 2070, 2358
PctI GAATGC 1 cut(s) 1378
PdmI GAANNNNTTC 1 cut(s) 157
PfeI GAWTC 7 cut(s) 875, 1259, 1460, 2355, 2378, 2690, 2904
PleI GAGTC 5 cut(s) 2, 539, 1082, 1300, 2141
PmaCI CACGTG 1 cut(s) 1488
PmlI CACGTG 1 cut(s) 1488
PpsI GAGTC 5 cut(s) 2, 539, 1082, 1300, 2141
Ppu21I YACGTR 2 cut(s) 1488, 3301
PscI ACATGT 2 cut(s) 2806, 2835
PshBI ATTAAT 1 cut(s) 1437
Psp124BI GAGCTC 3 cut(s) 2081, 2370, 3308
Psp6I CCWGG 5 cut(s) 141, 397, 868, 1777, 3052
PspCI CACGTG 1 cut(s) 1488
PspFI CCCAGC 2 cut(s) 692, 923
PspGI CCWGG 5 cut(s) 141, 397, 868, 1777, 3052
PspN4I GGNNCC 7 cut(s) 932, 1497, 2246, 2843, 2898, 3151, 3274
PspOMI GGGCCC 1 cut(s) 2841
PspPI GGNCC 7 cut(s) 471, 1237, 2841, 2842, 2897, 3157, 3272
PstI CTGCAG 2 cut(s) 2572, 2725
PstNI CAGNNNCTG 1 cut(s) 2198
PsuI RGATCY 3 cut(s) 1525, 2000, 2011
PvuII CAGCTG 2 cut(s) 692, 2198
RsaI GTAC 8 cut(s) 579, 703, 862, 932, 1193, 1398, 2507, 3299
RsaNI GTAC 8 cut(s) 578, 702, 861, 931, 1192, 1397, 2506, 3298
RseI CAYNNNNRTG 4 cut(s) 276, 297, 1199, 3005
SacI GAGCTC 3 cut(s) 2081, 2370, 3308
SapI GCTCTTC 2 cut(s) 2070, 2358
Sau96I GGNCC 7 cut(s) 471, 1237, 2841, 2842, 2897, 3157, 3272
ScaI AGTACT 1 cut(s) 862
SchI GAGTC 5 cut(s) 2, 540, 1082, 1301, 2141
ScrFI CCNGG 6 cut(s) 143, 399, 870, 1385, 1779, 3054
SduI GDGCHC 7 cut(s) 222, 445, 2065, 2081, 2370, 2845, 3308
SfcI CTRYAG 3 cut(s) 2568, 2721, 3240
SfuI TTCGAA 1 cut(s) 1531
SinI GGWCC 3 cut(s) 1237, 3157, 3272
SmiMI CAYNNNNRTG 4 cut(s) 276, 297, 1199, 3005
SmlI CTYRAG 2 cut(s) 2072, 2369
SmoI CTYRAG 2 cut(s) 2072, 2369
SseBI AGGCCT 1 cut(s) 557
SsiI CCGC 5 cut(s) 23, 1723, 1855, 2239, 2448
SspI AATATT 2 cut(s) 1762, 2395
SspMI CTAG 9 cut(s) 114, 497, 1182, 1695, 1793, 2211, 2799, 2901, 3119
SstI GAGCTC 3 cut(s) 2081, 2370, 3308
StuI AGGCCT 1 cut(s) 557
StyD4I CCNGG 6 cut(s) 141, 397, 868, 1383, 1777, 3052
StyI CCWWGG 2 cut(s) 474, 3084
TaaI ACNGT 2 cut(s) 1674, 1861
TaiI ACGT 6 cut(s) 1084, 1490, 2321, 2522, 2944, 3303
TaqI TCGA 6 cut(s) 445, 529, 1084, 1531, 2163, 2944
TaqII GACCGA 1 cut(s) 1857
TatI WGTACW 4 cut(s) 860, 1191, 1396, 2505
TauI GCSGC 2 cut(s) 1857, 2451
TfiI GAWTC 7 cut(s) 875, 1259, 1460, 2355, 2378, 2690, 2904
TscAI CASTG 5 cut(s) 473, 720, 1864, 2532, 3222
TseFI GTSAC 2 cut(s) 1716, 2480
TseI GCWGC 8 cut(s) 1316, 2195, 2445, 2567, 2570, 2720, 2723, 2771
Tsp45I GTSAC 2 cut(s) 1716, 2480
TspGWI ACGGA 2 cut(s) 237, 1626
TspRI CASTG 5 cut(s) 473, 720, 1864, 2532, 3222
VpaK11BI GGWCC 3 cut(s) 1237, 3157, 3272
VspI ATTAAT 1 cut(s) 1437
XbaI TCTAGA 3 cut(s) 496, 1694, 2210
XceI RCATGY 5 cut(s) 205, 1898, 2131, 2810, 2839
XcmI CCANNNNNNNNNTGG 1 cut(s) 712
XmiI GTMKAC 2 cut(s) 789, 2803
XmnI GAANNNNTTC 1 cut(s) 157
XspI CTAG 9 cut(s) 114, 497, 1182, 1695, 1793, 2211, 2799, 2901, 3119
ZrmI AGTACT 1 cut(s) 862
Zsp2I ATGCAT 2 cut(s) 207, 1174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.